# <i class="fa fa-gears"></i> Galaxy workflows for RNA-seq analysis
The following Galaxy workflows demonstrate how RNAcentral data can be used for differential expression analysis of RNA-seq datasets.
## Example Galaxy workflow
## Single-end RNA-seq workflow
Follow **Support Protocol 3** in the [RNAcentral paper](https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpbi.104) in *Current Protocols for Bioinformatics* for a step-by-step example showing how to use the [Galaxy](https://usegalaxy.org) platform and RNAcentral annotations to perform differential expression analysis of RNA-seq datasets from *Drosophila melanogaster*.
In addition, the following [Galaxy](https://usegalaxy.org) workflows demonstrate how RNAcentral data can be used for differential expression analysis of RNA-seq datasets.