We recommend that Fastq, BAM, and CRAM read files are submitted using Webin-CLI
When using this interface instead of Webin-CLI, raw sequences must be uploaded in one of the supported data formats before they can be submitted. The files can be uploaded using Webin File Uploader, FTP or Aspera
The study and the sequenced samples must be pre-registered before the raw reads are submitted. Please note that each individual study and sample should be registered only once. You will be asked to provide information about the sequencing libraries and instruments. Please quote the study accession number (ERP*) when citing data submitted to ENA.
A PacBio experiment output consists of a metadata.xml, bas.h5 and bax.h5 files. These files should be submitted using a 'manifest.all' file that lists these files for a given run. The recommended way to create the manifest file is to use the linux md5sum command. The manifest file must have two columns, the first containing file md5 checksum and the second the file name.
For example, the 'manifest.all' file may look like:
The 'manifest.all' file should be submitted to the archive on behalf of the files listed within.
Please download the spreadsheet template containing the fields you have selected by using the 'Download' button below.
If you have downloaded and filled a Read submission template spreadsheet please upload it using the Submit Completed Spreadsheet button.