From fc6ced683c9b544127e6f50711ba833b430baf88 Mon Sep 17 00:00:00 2001
From: Arne Stabenau <stabenau@sanger.ac.uk>
Date: Fri, 9 Jan 2004 13:50:02 +0000
Subject: [PATCH] Merged in the new-seqstore into HEAD version
---
docs/ensembl_changes_spec.txt | 958 +-
docs/schema_description/tables.html | 229 +-
docs/schema_description/tables.txt | 192 +-
docs/schema_description/tables.xml | 209 +-
docs/tutorial/ensembl_tutorial.doc | Bin 126464 -> 117248 bytes
docs/tutorial/ensembl_tutorial.pdf | Bin 126731 -> 36606 bytes
docs/tutorial/ensembl_tutorial.ps | 15813 +++++-----------
docs/tutorial/ensembl_tutorial.sxw | Bin 30204 -> 20098 bytes
docs/tutorial/ensembl_tutorial.txt | 1925 +-
docs/tutorial/tutorial.pl | 15 +-
misc-scripts/chain/chain2assembly.pl | 131 +
misc-scripts/qtl/extract_rat_map_ids.pl | 64 +
.../SeqStoreConverter/AnophelesGambiae.pm | 263 +
.../SeqStoreConverter/BasicConverter.pm | 1035 +
.../CaenorhabditisBriggsae.pm | 254 +
.../CaenorhabditisElegans.pm | 133 +
.../surgery/SeqStoreConverter/DanioRerio.pm | 335 +
.../DrosophilaMelanogaster.pm | 99 +
.../surgery/SeqStoreConverter/FuguRubripes.pm | 167 +
.../surgery/SeqStoreConverter/HomoSapiens.pm | 47 +
.../surgery/SeqStoreConverter/MusMusculus.pm | 211 +
.../SeqStoreConverter/PanTroglodytes.pm | 96 +
.../SeqStoreConverter/RattusNorvegicus.pm | 135 +
misc-scripts/surgery/convert_seqstore.pl | 124 +
misc-scripts/surgery/converter.input | 9 +
.../surgery/set_nonredundant_attribs.pl | 235 +
modules/Bio/EnsEMBL/Analysis.pm | 61 +-
.../Bio/EnsEMBL/AssemblyExceptionFeature.pm | 168 +
modules/Bio/EnsEMBL/AssemblyMapper.pm | 754 +-
modules/Bio/EnsEMBL/BaseAlignFeature.pm | 839 +-
modules/Bio/EnsEMBL/ChainedAssemblyMapper.pm | 548 +
modules/Bio/EnsEMBL/Chromosome.pm | 143 +-
modules/Bio/EnsEMBL/Clone.pm | 355 +-
modules/Bio/EnsEMBL/Container.pm | 21 +-
modules/Bio/EnsEMBL/CoordSystem.pm | 247 +
modules/Bio/EnsEMBL/DBEntry.pm | 119 +-
modules/Bio/EnsEMBL/DBSQL/AnalysisAdaptor.pm | 113 +-
.../DBSQL/AssemblyExceptionFeatureAdaptor.pm | 183 +
.../EnsEMBL/DBSQL/AssemblyMapperAdaptor.pm | 983 +-
modules/Bio/EnsEMBL/DBSQL/BaseAdaptor.pm | 13 +-
.../EnsEMBL/DBSQL/BaseAlignFeatureAdaptor.pm | 93 +-
.../Bio/EnsEMBL/DBSQL/BaseFeatureAdaptor.pm | 985 +-
.../Bio/EnsEMBL/DBSQL/ChromosomeAdaptor.pm | 151 +-
modules/Bio/EnsEMBL/DBSQL/CloneAdaptor.pm | 399 +-
.../DBSQL/CompressedSequenceAdaptor.pm | 186 +
.../Bio/EnsEMBL/DBSQL/CoordSystemAdaptor.pm | 902 +
modules/Bio/EnsEMBL/DBSQL/DBAdaptor.pm | 725 +-
modules/Bio/EnsEMBL/DBSQL/DBConnection.pm | 27 +-
modules/Bio/EnsEMBL/DBSQL/DBEntryAdaptor.pm | 239 +-
.../EnsEMBL/DBSQL/DensityFeatureAdaptor.pm | 432 +
.../EnsEMBL/DBSQL/DnaAlignFeatureAdaptor.pm | 380 +-
modules/Bio/EnsEMBL/DBSQL/ExonAdaptor.pm | 778 +-
modules/Bio/EnsEMBL/DBSQL/GeneAdaptor.pm | 1403 +-
.../Bio/EnsEMBL/DBSQL/KaryotypeBandAdaptor.pm | 233 +-
modules/Bio/EnsEMBL/DBSQL/MetaContainer.pm | 112 +-
.../Bio/EnsEMBL/DBSQL/MiscFeatureAdaptor.pm | 474 +
modules/Bio/EnsEMBL/DBSQL/MiscSetAdaptor.pm | 182 +
.../EnsEMBL/DBSQL/PredictionExonAdaptor.pm | 409 +
.../DBSQL/PredictionTranscriptAdaptor.pm | 538 +-
modules/Bio/EnsEMBL/DBSQL/ProteinAdaptor.pm | 180 +-
.../DBSQL/ProteinAlignFeatureAdaptor.pm | 414 +-
.../EnsEMBL/DBSQL/ProteinFeatureAdaptor.pm | 550 +-
modules/Bio/EnsEMBL/DBSQL/ProxyAdaptor.pm | 7 +-
modules/Bio/EnsEMBL/DBSQL/RawContigAdaptor.pm | 498 +-
.../EnsEMBL/DBSQL/RepeatConsensusAdaptor.pm | 89 +-
.../Bio/EnsEMBL/DBSQL/RepeatFeatureAdaptor.pm | 406 +-
modules/Bio/EnsEMBL/DBSQL/SequenceAdaptor.pm | 685 +-
.../Bio/EnsEMBL/DBSQL/SimpleFeatureAdaptor.pm | 240 +-
modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm | 1556 +-
.../EnsEMBL/DBSQL/SupportingFeatureAdaptor.pm | 16 +-
.../Bio/EnsEMBL/DBSQL/TranscriptAdaptor.pm | 845 +-
.../Bio/EnsEMBL/DBSQL/TranslationAdaptor.pm | 435 +-
modules/Bio/EnsEMBL/DensityFeature.pm | 191 +
modules/Bio/EnsEMBL/Exon.pm | 1267 +-
modules/Bio/EnsEMBL/External/BlastAdaptor.pm | 871 +-
.../External/ExternalFeatureAdaptor.pm | 566 +-
modules/Bio/EnsEMBL/Feature.pm | 767 +
modules/Bio/EnsEMBL/FeaturePair.pm | 772 +-
modules/Bio/EnsEMBL/Gene.pm | 800 +-
modules/Bio/EnsEMBL/IdentityXref.pm | 312 +-
modules/Bio/EnsEMBL/KaryotypeBand.pm | 192 +-
.../Lite/DensityPlot/DensityAdaptor.pm | 53 +-
modules/Bio/EnsEMBL/Lite/GeneAdaptor.pm | 5 +-
modules/Bio/EnsEMBL/Lite/SNPAdaptor.pm | 5 -
.../Bio/EnsEMBL/Map/DBSQL/MarkerAdaptor.pm | 28 +-
.../EnsEMBL/Map/DBSQL/MarkerFeatureAdaptor.pm | 260 +-
.../EnsEMBL/Map/DBSQL/QtlFeatureAdaptor.pm | 159 +-
modules/Bio/EnsEMBL/Map/MapLocation.pm | 109 +-
modules/Bio/EnsEMBL/Map/Marker.pm | 81 +-
modules/Bio/EnsEMBL/Map/MarkerFeature.pm | 102 +-
modules/Bio/EnsEMBL/Map/Qtl.pm | 85 +-
modules/Bio/EnsEMBL/Map/QtlFeature.pm | 95 +-
modules/Bio/EnsEMBL/Mapper.pm | 358 +-
modules/Bio/EnsEMBL/Mapper/Coordinate.pm | 5 +
modules/Bio/EnsEMBL/Mapper/Gap.pm | 7 +
modules/Bio/EnsEMBL/Mapper/RangeRegistry.pm | 268 +
modules/Bio/EnsEMBL/MiscFeature.pm | 263 +
modules/Bio/EnsEMBL/MiscSet.pm | 178 +
modules/Bio/EnsEMBL/PredictionExon.pm | 291 +
modules/Bio/EnsEMBL/PredictionTranscript.pm | 838 +-
modules/Bio/EnsEMBL/Protein.pm | 668 +-
modules/Bio/EnsEMBL/ProteinFeature.pm | 87 +-
modules/Bio/EnsEMBL/RawContig.pm | 742 +-
modules/Bio/EnsEMBL/RepeatConsensus.pm | 203 +-
modules/Bio/EnsEMBL/RepeatFeature.pm | 268 +-
modules/Bio/EnsEMBL/RepeatMaskedSlice.pm | 234 +
modules/Bio/EnsEMBL/Root.pm | 33 +-
modules/Bio/EnsEMBL/SNP.pm | 31 +-
modules/Bio/EnsEMBL/SeqFeature.pm | 49 +-
modules/Bio/EnsEMBL/SeqFeatureI.pm | 25 +-
modules/Bio/EnsEMBL/SimpleFeature.pm | 154 +-
modules/Bio/EnsEMBL/Slice.pm | 1741 +-
modules/Bio/EnsEMBL/StickyExon.pm | 43 +-
modules/Bio/EnsEMBL/Storable.pm | 171 +
modules/Bio/EnsEMBL/Tile.pm | 28 +-
modules/Bio/EnsEMBL/Transcript.pm | 1035 +-
modules/Bio/EnsEMBL/TranscriptFactory.pm | 16 +-
modules/Bio/EnsEMBL/Translation.pm | 324 +-
modules/Bio/EnsEMBL/Utils/Argument.pm | 162 +
modules/Bio/EnsEMBL/Utils/Converter.pm | 2 +-
modules/Bio/EnsEMBL/Utils/Exception.pm | 424 +
modules/Bio/EnsEMBL/Utils/SeqDumper.pm | 318 +-
modules/t/MultiTestDB.conf | 10 +-
modules/t/MultiTestDB.pm | 256 +-
modules/t/argument.t | 25 +
modules/t/assemblyException.t | 136 +
modules/t/assemblyExceptionFeature.t | 63 +
modules/t/assemblyMapper.t | 113 +
modules/t/canonicalDBAdaptor.t | 16 +-
modules/t/chainedAssemblyMapper.t | 146 +
modules/t/chromosome.t | 28 +-
modules/t/clone.t | 320 +-
modules/t/compressedSequenceAdaptor.t | 73 +
modules/t/coordSystem.t | 87 +
modules/t/coordSystemAdaptor.t | 155 +
modules/t/dbConnection.t | 2 -
modules/t/dbEntries.t | 58 +-
modules/t/densityFeature.t | 54 +
modules/t/densityFeatureAdaptor.t | 105 +
modules/t/dnaAlignFeatureAdaptor.t | 140 +-
modules/t/dnaDnaAlignFeature.t | 988 +-
modules/t/dnaPepAlignFeature.t | 584 +-
modules/t/exception.t | 86 +
modules/t/exon.t | 99 +-
modules/t/externalFeatureAdaptor.t | 146 +
modules/t/feature.t | 364 +
modules/t/featurePair.t | 97 +
modules/t/gene.t | 160 +-
modules/t/geneview.t | 2 +-
modules/t/karyotypeBand.t | 48 +-
modules/t/karyotypeBandAdaptor.t | 87 +
modules/t/mapLocation.t | 13 +-
modules/t/mapper.t | 90 +-
modules/t/marker.t | 4 +-
modules/t/markerFeature.t | 14 +-
modules/t/markerFeatureAdaptor.t | 15 +-
modules/t/metaContainer.t | 28 +-
modules/t/miscFeature.t | 87 +
modules/t/miscFeatureAdaptor.t | 106 +
modules/t/miscSet.t | 45 +
modules/t/miscSetAdaptor.t | 57 +
modules/t/predictionTranscript.t | 248 +-
modules/t/proteinAlignFeatureAdaptor.t | 140 +-
modules/t/proteinFeature.t | 86 +
modules/t/proteinFeatureAdaptor.t | 43 +
modules/t/qtl.t | 8 +-
modules/t/rangeRegistry.t | 115 +
modules/t/rawContig.t | 17 +-
modules/t/rawContigAdaptor.t | 175 +-
modules/t/repeatConsensus.t | 74 +-
modules/t/repeatConsensusAdaptor.t | 87 +
modules/t/repeatFeature.t | 135 +-
modules/t/repeatFeatureAdaptor.t | 126 +
modules/t/seqDumper.t | 67 +
modules/t/sequenceAdaptor.t | 70 +
modules/t/simpleFeature.t | 131 +-
modules/t/slice.t | 249 +-
modules/t/sliceAdaptor.t | 306 +-
modules/t/test_genome.zip | Bin 994753 -> 960638 bytes
modules/t/transcript.t | 81 +-
modules/t/translation.t | 66 +-
scripts/dna_compress.pl | 423 +-
sql/table.sql | 1287 +-
183 files changed, 36044 insertions(+), 30278 deletions(-)
create mode 100644 misc-scripts/chain/chain2assembly.pl
create mode 100644 misc-scripts/qtl/extract_rat_map_ids.pl
create mode 100644 misc-scripts/surgery/SeqStoreConverter/AnophelesGambiae.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/BasicConverter.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/CaenorhabditisBriggsae.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/CaenorhabditisElegans.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/DanioRerio.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/DrosophilaMelanogaster.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/FuguRubripes.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/HomoSapiens.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/MusMusculus.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/PanTroglodytes.pm
create mode 100644 misc-scripts/surgery/SeqStoreConverter/RattusNorvegicus.pm
create mode 100644 misc-scripts/surgery/convert_seqstore.pl
create mode 100644 misc-scripts/surgery/converter.input
create mode 100644 misc-scripts/surgery/set_nonredundant_attribs.pl
create mode 100644 modules/Bio/EnsEMBL/AssemblyExceptionFeature.pm
create mode 100644 modules/Bio/EnsEMBL/ChainedAssemblyMapper.pm
create mode 100644 modules/Bio/EnsEMBL/CoordSystem.pm
create mode 100644 modules/Bio/EnsEMBL/DBSQL/AssemblyExceptionFeatureAdaptor.pm
create mode 100644 modules/Bio/EnsEMBL/DBSQL/CompressedSequenceAdaptor.pm
create mode 100644 modules/Bio/EnsEMBL/DBSQL/CoordSystemAdaptor.pm
create mode 100644 modules/Bio/EnsEMBL/DBSQL/DensityFeatureAdaptor.pm
create mode 100644 modules/Bio/EnsEMBL/DBSQL/MiscFeatureAdaptor.pm
create mode 100644 modules/Bio/EnsEMBL/DBSQL/MiscSetAdaptor.pm
create mode 100644 modules/Bio/EnsEMBL/DBSQL/PredictionExonAdaptor.pm
create mode 100644 modules/Bio/EnsEMBL/DensityFeature.pm
create mode 100644 modules/Bio/EnsEMBL/Feature.pm
create mode 100644 modules/Bio/EnsEMBL/Mapper/RangeRegistry.pm
create mode 100644 modules/Bio/EnsEMBL/MiscFeature.pm
create mode 100644 modules/Bio/EnsEMBL/MiscSet.pm
create mode 100644 modules/Bio/EnsEMBL/PredictionExon.pm
create mode 100644 modules/Bio/EnsEMBL/RepeatMaskedSlice.pm
create mode 100644 modules/Bio/EnsEMBL/Storable.pm
create mode 100644 modules/Bio/EnsEMBL/Utils/Argument.pm
create mode 100644 modules/Bio/EnsEMBL/Utils/Exception.pm
create mode 100644 modules/t/argument.t
create mode 100644 modules/t/assemblyException.t
create mode 100644 modules/t/assemblyExceptionFeature.t
create mode 100644 modules/t/assemblyMapper.t
create mode 100644 modules/t/chainedAssemblyMapper.t
create mode 100644 modules/t/compressedSequenceAdaptor.t
create mode 100644 modules/t/coordSystem.t
create mode 100644 modules/t/coordSystemAdaptor.t
create mode 100644 modules/t/densityFeature.t
create mode 100644 modules/t/densityFeatureAdaptor.t
create mode 100644 modules/t/exception.t
create mode 100644 modules/t/externalFeatureAdaptor.t
create mode 100644 modules/t/feature.t
create mode 100644 modules/t/featurePair.t
create mode 100644 modules/t/karyotypeBandAdaptor.t
create mode 100644 modules/t/miscFeature.t
create mode 100644 modules/t/miscFeatureAdaptor.t
create mode 100644 modules/t/miscSet.t
create mode 100644 modules/t/miscSetAdaptor.t
create mode 100644 modules/t/proteinFeature.t
create mode 100644 modules/t/proteinFeatureAdaptor.t
create mode 100644 modules/t/rangeRegistry.t
create mode 100644 modules/t/repeatConsensusAdaptor.t
create mode 100644 modules/t/repeatFeatureAdaptor.t
create mode 100644 modules/t/seqDumper.t
create mode 100644 modules/t/sequenceAdaptor.t
diff --git a/docs/ensembl_changes_spec.txt b/docs/ensembl_changes_spec.txt
index 40f505f83c..2153b7c4c6 100644
--- a/docs/ensembl_changes_spec.txt
+++ b/docs/ensembl_changes_spec.txt
@@ -1,3 +1,955 @@
-The most up-to-date version of this document can be found on
-branch-new-seqstore. It has been moved there so it does not have to be
-maintained in two locations.
\ No newline at end of file
+ENSEMBL - API Change Specification
+==================================
+
+CONTENTS
+--------
+
+Introduction
+Goals
+Schema Modifications
+ Proposed New/Modified Tables
+ seq_region
+ coord_system
+ seq_region_annotation
+ dna
+ assembly
+ gene
+ transcript
+ translation
+ all feature tables
+ meta_coord
+ misc_feature
+ misc_set
+ misc_feature_misc_set
+ misc_attrib
+ Removed Tables
+ contig
+ clone
+ chromosome
+Meta Information
+API Changes
+ Slice
+ Tile
+ SliceAdaptor
+ RawContig
+ RawContigAdaptor
+ Clone
+ CloneAdaptor
+ Chromosome
+ ChromosomeAdaptor
+ Root
+ Storable Base Class
+ Features
+ transform
+ transfer
+ move
+ project
+ StickyExon
+ AssemblyMapper
+ FeatureAdaptors
+ CoordSystemAdaptor
+New Features
+ Assembly Exceptions
+ Haplotypes
+ Pseudo Autosomal Regions
+ Multiple Assemblies
+Other Considerations
+ Loci
+
+
+INTRODUCTION
+------------
+
+This document describes the changes that are being made to the EnsEMBL core
+schema and Perl/Java/C APIs.
+
+GOALS
+-----
+-A cleaner, more intuitive API
+-A more general schema able to better capture divergent assembly types
+-More flexibility with regards to assembly related data such as haplotypes,
+ PARs, WGS assemblies etc.
+
+SCHEMA MODIFICATIONS
+--------------------
+
+Proposed New/Modified Tables:
+-----------------------------
+
+ seq_region
+ ----------
+ The seq_region table is a generic replacement for the clone, contig,
+ and chromosome tables. Additionally supercontigs which were formerly in the
+ assembly table are also present in this table. The name column can contain
+ chromosome names, clone accessions, supercontig names or anything that is
+ appropriate for the seq_region it describes. The coord_system_id is a
+ foreign key to the new coordinate system and is used to distinguish
+ between the divergent types of sequence regions in the table.
+
+ seq_region_id int
+ name varchar
+ coord_system_id int references coord_system table
+ length int
+
+
+ coord_system
+ ------------
+ The coordinate system table lists the available coordinate systems in the
+ database. The attrib is mysql set and is used to denote the default version
+ of each named coordinate system. E.g. there may be two 'chromosome' coordiate
+ systems and the default may be version 'NCBI34'. The 'top_level' and
+ sequence level attribs denote the coordinate system from which sequence is
+ retrieved and the coordinate system which has the largest assembled pieces.
+ The top_level coordinate system will usually be 'chromosome' but for some
+ shrapnel assemblied this may be something like 'supercontig' or 'clone'.
+
+ There may be multiple toplevel coordinate systems providing that they share
+ the same name (but different version) and providing one of them is the
+ default. There may only be a single sequence level coordinate system.
+
+ Note that the version in the coordinate system can be viewed as applying
+ to every seq_region of a given coordinate system. It is analagous to
+ a CVS tag, not a CVS version. E.g. The version would 'NCBI33' apply to
+ every chromosome seq_region so it is a valid version. A clone accession of
+ '8' would not be a valid version because it only describes a particular
+ seq_region of the coordinate system - not all of them.
+
+ coord_system_id int
+ name varchar
+ version varchar
+ attrib set ('top_level', 'default_version', 'sequence_level')
+
+
+ seq_region_annotation
+ ---------------------
+ This table allows for extra arbitrary information to be attached to
+ seq_regions. For example the htg_phase was formerly part of the clone table
+ but now is stored in this table.
+
+ seq_region_id int
+ attrib_type_id smallint references attrib_type table
+ value varchar
+
+
+ dna
+ ---
+ Formerly the contig table referenced the dna table. Now the dna table
+ refrences the seq_region_table. Every seq_region which has a coordinate
+ system with the 'sequence_level' attrib should be referenced by an entry in
+ the dna table.
+
+ seq_region_id int
+ sequence varchar
+
+
+ assembly
+ --------
+ The assembly table has been made more generic. Columns that previously
+ were names chr_* and contig_* have been renamed asm_* and cmp_* (assembled
+ and component) respectively. The superctg_name column has been removed.
+ Supercontigs are now defined in the seq_region table.
+
+ The makeup of all seq_regions from smaller seq_regions can be described in
+ this table. The relationships which are explicitly defined must be listed
+ in the meta table. For example, the clone <-> contig mapping used to be
+ defined in the contig table with an embl_offset column. This information is
+ now found in this table instead.
+
+ asm_seq_region_id int
+ asm_start int
+ asm_end int
+ cmp_seq_region_id int
+ cmp_start int
+ cmp_end int
+ ori tinyint
+
+ gene
+ ----
+ For faster retrieval and retrieval independently of transcripts and
+ exons, genes have a seq_region_id, seq_region_start and seq_region_end
+ which defines the span of their transcript.
+
+ The transcript_count column has been removed as it was never used.
+
+ gene_id int
+ type varchar
+ analysis_id int
+ seq_region_id int
+ seq_region_start int
+ seq_region_end int
+ seq_region_strand tinyint
+ display_xref_id int
+
+
+ transcript
+ ----------
+ For faster retrieval and retrieval independently of genes and exons
+ transcripts also have a seq_region_id, seq_region_start and
+ seq_region_end. The translation_id has been removed; translations will point
+ to transcripts instead (and pseudogenes will have no translation).
+
+ The exon_count column has been removed as it was never used.
+
+ transcript_id int
+ gene_id int
+ seq_region_id int
+ seq_region_start int
+ seq_region_end int
+ seq_region_strand tinyint
+ display_xref_id int
+
+
+ translation
+ -----------
+ Translations now reference transcripts rather than transcripts referencing
+ a single (or no) translation. This allows for more elegant handling of
+ pseudogenes (where there is no translation) and also can be used to supply
+ multiple translations for a single transcript (e.g. polycistronic genes).
+
+ translation_id int
+ transcript_id int
+ start_exon_id int
+ end_exon_id int
+ seq_start int
+ seq_end int
+
+
+ all feature tables
+ ------------------
+ All feature tables would now have seq_region_id, seq_region_start,
+ seq_region_end, seq_region_strand instead of contig_id, contig_start,
+ contig_end. This includes the repeat_feature, simple_feature,
+ dna_align_feature, protein_align_feature, exon, marker_feature,
+ karyotype and qtl_feature tables.
+
+ meta_coord
+ ----------
+ The meta coord table defines what coordinate systems are used to store each
+ type of feature. A given type of feature may be stored in multiple
+ coordinate systems, but these will not be retrieved by the API unless there
+ is an entry in the meta_coord table.
+
+ table_name varchar
+ coord_system_id int
+
+
+ misc_feature
+ ------------
+ This is a renaming of the mapfrag table. The renaming reflects the fact that
+ this table can be used to store any type of feature.
+
+ misc_feature_id
+ seq_region_id
+ seq_region_start
+ seq_region_end
+ seq_region_strand
+
+ misc_set
+ --------
+ This table was formerly names mapset. It defines 'sets' that can be used
+ to group misc_features together.
+
+ misc_set_id smallint
+ code varchar
+ name description
+ description text
+ max_length int
+
+ misc_feature_misc_set
+ ---------------------
+ This is a link table defining the many-to-many relationship between the
+ misc_set and misc_feature tables.
+
+ misc_feature_id int
+ misc_set_id smallint
+
+
+ misc_attrib
+ -----------
+ This table was formerly named mapfrag_annotation. It contains arbitrary
+ annotations of misc_features and links to the same attrib_type table that
+ the seq_region_attrib table uses.
+
+ misc_feature_id int
+ attrib_type_id smallint
+ value varchar
+
+
+
+Removed Tables
+--------------
+
+ contig
+ ------
+ Contigs are no longer needed. They are stored as entries in the seq_region
+ table with type 'contig'. The embl_offset and clone_id will not be
+ necessary as their relationship to clones can be described by the
+ assembly table.
+
+ clone
+ -----
+ Clones are no longer needed. Clones are stored as entries in the seq_region
+ table with coord_system 'clone'. The modified timestamp will be discarded
+ as it is no longer maintained anyway. The embl_acc, version, and
+ embl_version columns are redundant and will also be discarded. Versions
+ are simply appended onto the end of the name with a delimiting '.'.
+
+ Any additional information that needs to be present (such as htg_phase) can
+ be added to the seq_region_attrib table.
+
+ chromosome
+ ----------
+ This table is no longer needed. Chromosomes can be stored in the
+ seq_region table with a 'chromosome' coord_system.
+
+
+
+META INFORMATION
+----------------
+
+Considerable more meta information is stored in the core
+databases in order for the general approach to be maintained.
+This information is stored in the new coord_system table and in the
+meta, and meta_coord tables.
+
+Meta information includes the following:
+
+ * The coordinate system that features of a given type are stored in. This
+ information is stored in the meta_coord table and is used when constructing
+ queries for a particular feature table.
+
+ * The top-level coordinate system. For human
+ this would be 'chromosome'. For briggsae this may be something like
+ 'scaffold' or 'super contig'. This information would be used to construct
+ the web display and would possibly be the default coordinate system when
+ a coordinate system is unspecified by a user. This is stored as a flag
+ in the coord_system table.
+
+ * The default version of each coordinate system. This is stored as a flag
+ in the coord_system table.
+
+ * The coord_system where sequence is stored. This will be stored as a
+ flag in the coord_system table. Initially it will only be possible
+ to have a single coord_system in which sequence is stored. This
+ may be extended in the future to allow sequence to be stored for multiple
+ coord_systems.
+
+ * The coordinate system relationships between that are explicitly defined
+ in the assembly table. The new API is capable of 2 step (implicit) mapping
+ between coordinate systems, but these relationships can be determined
+ through the direct relationship information.
+
+ For example the clone, chromosome and nt_contig coordinate systems may all
+ be constructed from the contig coordinate system:
+ contig -> clone
+ contig -> chromosome
+ contig -> nt_contig
+ Or there may be a more hierarchical approach:
+ contig -> clone
+ clone -> nt_contig
+ nt_contig -> chromosome
+ This information is stored in the meta table under the key
+ 'assembly.mapping' with the following format (versions are optional):
+ assembled_coord_system_name[:version]|component_coord_system_name[:version]
+
+ For example the meta table for human might contain the following entries:
+ mysql> select * from meta where meta_key = 'assembly.mapping';
+ +---------+------------------+--------------------------+
+ | meta_id | meta_key | meta_value |
+ +---------+------------------+--------------------------+
+ | 43 | assembly.mapping | chromosome:NCBI33|contig |
+ | 44 | assembly.mapping | clone|contig |
+ | 45 | assembly.mapping | supercontig|contig |
+ +---------+------------------+--------------------------+
+
+ * The names of the allowable coordinate systems. This would allow for
+ quick validation of API requests and provide a list that could be used
+ by the website for coordinate system selection. This information will be
+ stored in the coord_system table.
+
+ * The coordinate system(s) that each feature type is stored in. This is
+ stored in the meta_coord table.
+
+
+API CHANGES
+-----------
+
+Slice
+-----
+ Slice methods chr_start, chr_end, chr_name will be renamed start, end,
+ seq_region_name. For backwards compatibility the old methods are
+ chained to the new methods with deprecated warnings.
+
+ A new slice method 'coord_system' will be added and will return a
+ Bio::EnsEMBL::CoordSystem object.
+
+ Slices will represent a region on a seq_region as opposed to a region on a
+ chromosome. Slices will be immutable (i.e. their attributes will not be
+ changeable). A new slice will have to be created if the attributes are to
+ be changed.
+
+ The following attributes will therefore define a unique slice:
+ coord_system (e.g. object with name and version)
+ seq_region_name (e.g. 'X' or 'AL035554.1')
+ start (e.g. 1000000 or 1)
+ end (e.g. 2000001 or 800)
+ strand (e.g. 1 or -1)
+
+ The name method will return the above values joined by a ':' delimiter, and
+ will not be settable:
+ e.g. 'chromosome:NCBI33:X:1000000:2000001:1' or 'clone::AL035554.1:1:800:-1'
+ This value can be used as a hashvalue that uniquely defines a slice.
+
+ The concept of an 'empty' slice will no longer exist.
+
+ The get_tiling_path method will be deprecated in favour of a more general
+ method project(). Whereas get_tiling_path() implies a relationship between
+ an assembly and the coordinate system which makes up the assembly the
+ project method will allow conversion accross any two coordinate systems.
+ It will take a coord_system string as an argument and rather
+ than returning a list of Tile objects it will return a listref of triplets
+ containing a start int, and end int, and a 'to' slice object. The following
+ is an example of how this method would be used ($clone is a reference to a
+ slice object in the clone coordinate system):
+
+ my $clone_path = $slice->project('clone');
+
+ foreach my $segment (@$clone_path) {
+ my ($start, $end, $clone) = @$segment;
+ print $slice->seq_region_name, ':', $start, '-', $end , ' -> ',
+ $clone->seq_region_name, ':', $clone->start, '-', $clone->end,
+ $clone->strand, "\n";
+ }
+
+ An optional second argument to project() will be the coordinate
+ system version. E.g.:
+ $ncbi34_path = $slice->project('chromosome','NCBI34').
+
+
+Tile
+----
+ The tile object will no longer be necessary. However for backwards
+ compatibility it will remain in the system for some time before being phased
+ out along with the get_tiling_path method.
+
+
+SliceAdaptor
+------------
+ The Slice adaptor must provide a method to fetch a slice via its coordinate
+ system, seq_region_name, start, end, and strand.
+ The old, commonly used method fetch_by_chr_start_end has been altered to
+ simply chain to this new method (with a warning) as do most other
+ SliceAdaptor methods.
+
+ Another method which is necessary with the disapearence of the Clone,
+ RawContig and Chromosome adaptors is one which allows for all slices
+ of a certain type to be retrieved. For example it is often necessary to
+ retrieve all chromosomes, or clones for a species. This method is simply
+ named fetch_all. The old fetch_all methods on the ChromosomeAdaptor,
+ RawContigAdaptor, CloneAdaptor, etc. chain to the new method for backwards
+ compatibility.
+
+ Method Names and Signatures
+ ---------------------------
+ Slice fetch_by_region(coord_system, name)
+ Slice fetch_by_region(coord_system, name, start)
+ Slice fetch_by_region(coord_system, name, start, end)
+ Slice fetch_by_region(coord_system, name, start, end, strand)
+ Slice fetch_by_region(coord_system, name, start, end, strand, version)
+ listref of Slices fetch_all(coord_system)
+ listref of Slices fetch_all(coord_system, version)
+
+RawContig
+---------
+ The RawContig object is no longer necessary with the new system. RawContigs
+ are replaced by Slices with coord_system = 'contig'. In the interests of
+ backwards compatibility the RawContig class will still be present for
+ sometime as a minimal implmentation inheriting from the Slice class.
+
+
+RawContigAdaptor
+----------------
+ The RawContigAdaptor is no longer necessary. The RawContigAdaptor is
+ replaced by the SliceAdaptor. For backwards compatibility a minimal
+ implementation of the RawContigAdaptor will remain which inherits from the
+ SliceAdaptor.
+
+Clone
+-----
+ The Clone object is no longer necessary in the new system. Clones are
+ replaced by Slices with coord_system = 'clone'. For backwards compatibility
+ a minimal implementation will remain which inherits from the Slice object.
+
+CloneAdaptor
+------------
+ The CloneAdaptor object is no longer necessary in the new system. The
+ CloneAdaptor is replaced by the SliceAdaptor. For backwards compatibility
+ a minimal implementation will remain which inherits from the SliceAdaptor.
+
+Chromosome
+----------
+ The Chromosome object is no longer necessary in the new system. The
+ Chromosome is replaced by Slices with coord system 'chromosome' (or
+ whatever the top level seq_region type is for that species). For backwards
+ compatibility a minimal implementation will remain which inherits from the
+ Slice object.
+
+ Statistical information (e.g. known genes, genes, snps) that
+ was on chromosomes may be stored in the seq_region_attrib table or
+ in some sort of density table.
+
+ChromosomeAdaptor
+-----------------
+ The Chromosome object is no longer necessary in the new system. The
+ ChromosomeAdaptor is replaced by the SliceAdaptor. For backwards
+ compatibility a minimal implementation which inherits from the SliceAdaptor
+ will remain.
+
+
+Root
+----
+ Every class in the current EnsEMBL perl API inherits directly or indirectly
+ from Bio::EnsEMBL::Root. This inheritance is almost exclusively for the
+ following following three methods:
+ throw
+ warn
+ _rearrange
+
+ Nothing is gained by implementing this relationship as inheritance, and there
+ are several disadvantages:
+ (1) Everything must inherit from this class to use those 3 object methods.
+ This can result in patterns of multiple inheritance which are generally
+ considered to be a bad thing.
+
+ (2) It is not possible to use the throw, warn or rearrange method within
+ the constructor until the object is blessed. Blessing the object first
+ and then calling rearrange to extract named arguments is slower because
+ the blessed hash needs to be expanded as more keys are added and several
+ key access/value assignements may need to be performed.
+
+ (3) Objects become larger and object construction becomes slightly slower
+ because constructors traverse an additional level of inheritance.
+
+ A better approach, which we have used, is to make the methods static and
+ create a static utility class that exports the methods. The warn method has
+ been renamed warning so as not to conflict with the builtin perl function
+ warn and the _rearrange method has be renamed rearrange.
+
+ The following is an example of the old styl Root inheritance and the new
+ style static utility methods:
+
+ #
+ # OLD STYLE
+ #
+ package Old;
+
+ use Bio::EnsEMBL::Root;
+
+ @ISA = qw(Bio::EnsEMBL::Root);
+
+ sub new {
+ my $caller = shift;
+ my $class = ref($caller) || $caller;
+
+ $self = $class->SUPER::new(@_);
+
+ my ($start, $end) = $self->_rearrange(['START', 'END'], @_);
+
+ if(!defined($start) || !$defined($end)) {
+ $self->throw('-START and -END arguments are required');
+ }
+
+ $self->{'start'} = $start;
+ $self->{'end'} = $end;
+
+ return $self;
+ }
+
+ #
+ # NEW STYLE
+ #
+ package New;
+
+ use Bio::EnsEMBL::Utils::Exception qw(throw warning);
+ use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+ sub new {
+ my $caller = shift;
+
+ my $class = ref($caller) || $caller;
+
+ my ($start, $end) = rearrange(['START', 'END'], @_);
+
+ if(!defined($start) || !defined($end)) {
+ throw('-START and -END arguments are required');
+ }
+
+ return bless {'start' => $start, 'end' => $end}, $class;
+ }
+
+ The calls to $self->rearrange $self->warn and $self->throw have been
+ replaced by class method calls to warning() and throw() inside the core API.
+ However, for backwards compatibility the existance inheritance to
+ Bio::EnsEMBL::Root will remain in many cases (and be removed at a later date)
+
+
+Storable Base Class
+-------------------
+ Almost all business objects in the EnsEMBL system are storable in the db
+ and the ones which are always require 2 methods: dbID() and adaptor(). These
+ methods have been moved to a Storable base class which most of the
+ business objects now inherit from. This module has an additional method
+ is_stored() which takes a database argument and returns true if the object
+ appears to already have been stored in the provided database.
+
+Features
+--------
+ All features should inherit from a base class that implements common feature
+ functionality. Formerly this role was filled by the bloated SeqFeature class
+ which inherits from Bio::SeqFeature and Bio::SeqFeatureI etc.
+ This class has been replaced by a smaller, less complicated
+ implementation named Feature. To make classes more polymorphic in general,
+ the gene, and transcript objects should now also inherit from the Feature
+ class. This class implements the following core methods common to all
+ features:
+
+ start
+ end
+ strand
+ slice (formerly named contig/entire_seq/etc.)
+ transform
+ transfer
+ project
+ analysis
+
+ The feature class inherits from the Storable base class and thereby inherits
+ the following methods:
+
+ adaptor
+ dbID
+ is_stored
+
+ The signature and behaviour of the transform method has been changed. The
+ existing method works differently depending on the arguments passed as
+ described below.
+
+ OLD transform(no arguments)
+ -----------------------
+ Transforms from slice coordinates to contig coordinates. The feature
+ is changed in place and returned. If the feature already is in contig
+ coordinates an exception is thrown. The feature may be split into two
+ features in which case both features are returned (not sure if one of
+ them is transformed in place). Some features are not permitted to be
+ split in two in which case an exception is thrown? (not sure) if it is
+ to be split accross contigs.
+
+ OLD transform(slice)
+ ----------------
+ If the feature is already in slice coordinates and the slice is on the
+ same chromosome the features coordinates are simply shifted. If the
+ feature is already in slice coordinates but on a different chromosome
+ an exception is thrown.
+ It the feature is in contig coordinates and the slice is not empty then
+ it is transformed onto the new slice (or an exception is thrown if the
+ transform would cause the feature to end up on a different chromosome
+ than the slice). If the feature is in contig coordinates and the
+ slice is an empty slice the feature is transformed into chromosomal
+ coordinates and placed on a newly created slice of the entire chromosome.
+
+ The new transformation has only a single valid signature and splits its
+ responsibilities with the new transfer method. The transfer
+ method transfers a feature onto another slice, whereas the transform
+ method simply converts coordinate systems. Transform does
+ NOT transform features in place but rather returns the newly
+ transformed feature as a new object:
+
+ transform(coord_system, [version])
+ -----------------------
+ Takes a single string specifying the new coord system. If the coord
+ system is not valid an exception is thrown. If the coord system is the
+ same coord system as the feature is currently in a new feature that is
+ a copy of the old one is still be returned. This also retrieves
+ a slice which is the entire span of the region of the coordinate system
+ that this feature is being transformed to. For example transforming
+ an exon in contig coordinates to chromosomal coodinates will place a
+ copied exon on a slice of an entire chromosome. If a feature spans a
+ boundary in the coordinate system, undef is returned by the method
+ instead.
+
+ transfer(slice)
+ ----------------
+ Shifts a feature from one slice to another. If the new slice is in the
+ same coordinate system but different seq_region_name (e.g. both
+ chromosomal but different chromosomes) an exception is thrown.
+ If the new slice is in a different coordinate system then the
+ transform method is internally called first. If the feature would be
+ split across a boundary undef is returned instead. After the transform
+ there follows a potential move, if the slice does not cover the full
+ seq_region. If there is no transform call necessary, the feature is
+ copied and then moved.
+
+ move( start, end, strand )
+ --------------------------
+ In place change of the coordinates of the feature. It will stay on the
+ same slice.
+
+ project(coord_system, [version])
+ -----------------
+ This method is analagous to the project method on Bio::EnsEMBL::Slice.
+ It 'projects' a feature onto another coordinate system and returns the
+ results formatted as a listref of [$start, $end, $feature] triplets.
+ The $features returned are copies of the feature on which the method was
+ called, but with coordinates in the coordinate system that was projected
+ to. If the feature maps entirely to a gap then an empty list ref [] will
+ be returned. If the feature is mapped to multiple locations a listref
+ containing split features will be returned.
+
+StickyExon
+----------
+ The sticky exon object is not be present in the new system. It does not
+ make sense to define features in a coordinate system where they are simply
+ not present. Exons are calculated in chromosomal coordinates and they
+ will generally be retrieved in the same coordinates system. It will
+ of course be possible to still retrieve exons in contig coordinates but only
+ is they are fully defined on the contigs of interest.
+ The split coordinates can be obtained through a call to the project
+ method.
+
+
+AssemblyMapper
+--------------
+ The assembly mapper and assembly mapper adaptor classes have become more
+ general and sophisticated.Not only is it possible to map between two
+ coordinates systems whose relationship is explicitly defined in the assembly
+ table, but it is also possible to perform implicit, 2-step mapping
+ using 'coordinate system chaining'.
+
+ For example if no explicit relationship is defined between the supercontig
+ and clone coordinate systems but relationships between the clone and contig
+ and the supercontig and contig coordinate systems is present the mapper has
+ the faculty to perform the mapping between the clone and supercontig systems.
+ In this case the contig cooridinate system is used as an intermediary:
+
+ NTContig <-> Contig <-> Clone
+
+ In the above example the assembly mapper adaptor internally does the
+ following:
+
+ (1) Create a mapper object between the NTContig and Contig region
+ (2) Create a mapper object between the Contig and Clone region
+ (3) Create and return a third mapper constructed from the sets of mappings
+ generated by the intermediate mappers.
+
+
+FeatureAdaptors
+---------------
+ Most FeatureAdaptors inherit from the BaseFeature adaptor. As a
+ minimum feature adaptors provide fetch_all_by_Slice and fetch_by_dbID
+ methods. The fetch by slice method provides the same return types and
+ requires the same arguments as before, but required some internal changes.
+
+ The simplified algorithm for fetching features via a slice is:
+
+ (1) Check with coord system is requested or that slice is in.
+ (2) Check which coord system features are in
+ (3) Obtain mapper between coord systems
+ (5) Retrieve features in their native coord system.
+ (6) Remap features to the requested coord system using the mapper
+ (7) Return the features
+
+ The method fetch_all_by_RawContig is obsolete (it is equivalent to
+ fetching by a slice of a contig) but has be left in as an alias for the
+ fetch all by slice method for backwards compatibility.
+
+ When performing a non-locational fetch (e.g. by dbID) features are still
+ returned in the coordinate system that they are calculated in. This is to
+ ensure that the feature can always be retreived in this manner of fetching
+ and so that features which are not in the database can be distinguished from
+ features which are simply not in the requested coordinate sytem. When a
+ single feature which is not in the database is requested via a non-locational
+ fetch undef is returned instead. If multiple features are requested but none
+ are present in the database a reference to an empty list is returned. If the
+ features are required in a specific coordinate system the transfer, project
+ or transform method can always be used.
+
+
+CoordSystemAdaptor
+------------------
+ A CoordSystemAdaptor provides access to the information in the
+ coord_system, meta and meta_coord tables. This adaptor provides
+ Bio::EnsEMBL::CoordSystem objects.
+
+
+NEW FEATURES
+------------
+
+Assembly Exceptions (Symbolic Sequence Links)
+---------------------------------------------
+
+ It is sometimes desirable to have multiple regions refer to the same
+ sequence.
+
+ In much the same way a symlinked file acts as a pointer to a real file,
+ a symlinked region can point to another region of sequence.
+
+ This can be described in the database through the addition of a table which
+ has a structure that mirrors that of the assembly table. The assembly table
+ does not define the structure underlying this seq_region, and it does not
+ have sequence of its own. By means of the assembly_exception table this
+ seq_region points to another seq_region where the underlying sequence is
+ defined:
+
+ assembly_exception
+ ------------------
+ seq_region_id int
+ seq_region_start int
+ seq_region_end int
+ exc_type enum('HAP', 'PAR')
+ exc_seq_region_id int
+ exc_seq_region_start int
+ exc_seq_region_end int
+ ori int (may not be needed, may implicitly be 1)
+
+ When fetching features and sequence from a slice that overlaps a symlinked
+ region, the features and sequence from the symlinked region are returned.
+ This may be implemented by altering fetch by slice calls and adding a
+ SliceAdaptor method with splits a slice into non-symlinked components.
+ The following algorithm would apply to sequence and feature fetches:
+ (1) Split the slice into non-symlinked component slices
+ (2) Recursively call the method with the component slices
+ (3) Adjust the start and end of the returned features and place them
+ back on the original slice (or splice the sequence together if this
+ is a sequence fetch)
+ (4) Return the features or sequence
+
+ Consider a slice which overlaps regions (A), (B), and (C) on chromosome Y:
+
+ =============== (chrX)
+ ^^^^^^^^^^^
+ ======== symlink ========= (chrY)
+ (A) (B) (C)
+
+ Regions (A) and (C) are described by the assembly table, but region (B)
+ is described in the assembly_exception table and points to a region of
+ chromosome Y. When features or sequence are retrieved the slice is split
+ into 3 component slices which have no symlinks: region (A) and (C) are
+ slices on chromosome Y but region (B) is made into a slice on chromsome (X).
+ All of the features are fetched from the individual slices adjusted by
+ some addition and placed on back on the original Slice before being
+ returned.
+
+
+
+Haplotypes (and the MHC region)
+-------------------------------
+ There are several requirements related to haplotypes:
+ - Must be able to determine which haplotypes overlap a slice
+ - Must be able to run genebuild/raw computes over the haplotypes
+ - Must be able to retrieve a slice on a haplotype and its flanking
+ regions (i.e. the regions of the default assembly bordering the
+ haplotype).
+ - It may be desireable to interpolate features from the default sequence
+ onto the haplotype
+
+ Proposal:
+ The haplotype will be present as a full length 'chromosome' in the
+ seq_region table (or other appropriate coordinate system) but only the
+ region which differs from the the default assembly will be described in
+ the chromosome table. The regions which are identical will be described
+ by the assembly_exception table.
+
+ It is possible to retrieve a slice on a haplotype just as any other slice
+ is retrieved from the SliceAdaptor. For example:
+ $slice = $slice_adaptor->fetch_by_region('chromosome', '6_DR52');
+
+ A slice created on a haplotype will have coordinates relative to the
+ start of the chromosome NOT relative to the start of the haplotype
+ region. For all intents and purposes a haplotype slice will behave as
+ a normal slice.
+
+ For example, the assembly table could define the composition of the
+ divergent region of chromosome 6_DR52 (C), but leave the remainder of the
+ chromosomal composition undefined. The remainder of the
+ chromosome composition would be accounted for by 2 rows in the
+ assembly_exception table which described the synonymous regions in terms
+ of chromosome 6:
+
+ ============== 6 ============== 6
+ ^ ^
+ _____|________ _______|______
+ C ========== 6_DR52
+
+
+
+
+
+Pseudo Autosomal Regions (PARs)
+-------------------------------
+ There are several requirements related to PARs:
+ - The same sequence and features must be present on a region of
+ both chromosome X and chromosome Y
+ - The region and features should be returned when retreiving features
+ from either chromosome.
+ - It must still be possible to retrieve one of the features via its
+ identifier
+ - It must still be possible to transform features in the region from
+ chromosomal coordinates to contig coords and vice-versa.
+ - The genebuild should run over the region, but only once.
+
+ Proposal:
+ Use the assembly_exception table in a similar fashion as it is used
+ for the haplotypes described above. Chromosome X can be the 'default'
+ chromosome for the PAR and Chromosome Y can be described by the assembly
+ table except in the PAR. The PAR on chromosome Y can be defined by the
+ assembly_exception table and refer to the corresponding sequence on
+ chromosome X. The same algorithm as used for haplotypes can then be used
+ when retrieving sequence or features from slices which overlap this
+ exception on chromosome Y.
+
+ The following diagram illustrates how chromsome X and chromosome Y could
+ be defined:
+
+ ========================================== X
+ ^ ^
+ _|_ ____|____
+ ====== ============ ============= Y
+
+
+Multiple Assemblies
+-------------------
+
+In theory it is possible to load multiple assemblies into the same database.
+For example two coordinate systems with two versions chromosome:NCBI33 and
+chromosome:NCBI34 could be loaded into the database. Leveraging the fact that
+two step mapping is possible and that these coordinate systems share a
+coincident mapping with the contig coorinate system it is possible to pull
+across annotation from one assembly to the other. The following example
+illustrates the transfer of genes from the chromosome X on the NCBI33 assembly
+to the NCBI34 assembly:
+
+ $slice = $slice_adaptor->fetch_by_region('chromosome', 'X', undef,
+ undef,undef, 'NCBI33');
+ @genes = @{$gene_adaptor->fetch_all_by_Slice($slice)};
+
+ foreach my $gene (@genes) {
+ $gene->transform('chromosome', 'NCBI34');
+ #...
+ }
+
+
+OTHER CONSIDERATIONS
+--------------------
+
+Loci
+----
+
+Similar genes which are defined across haplotypes need
+to be somehow linked into loci. The intent is that
+a user would be able to see that a gene has a counterpart
+on an equivalent haplotypic sequence.
+
+This is work in progress. The current opinion among us is to implement
+it via a relationship table that specifies which genes on default
+haplotypes are considered equivalent to other genes on
+(overlapping) haplotypes.
+
diff --git a/docs/schema_description/tables.html b/docs/schema_description/tables.html
index d59d496b64..58c3aca912 100644
--- a/docs/schema_description/tables.html
+++ b/docs/schema_description/tables.html
@@ -48,7 +48,7 @@
</tr>
</table>
</p>
- <p>This document refers to version <strong>15</strong> of the EnsEMBL core schema. You are looking at revision <strong>1.21</strong> of this document.
+ <p>This document refers to version <strong>15</strong> of the EnsEMBL core schema. You are looking at revision <strong>$Revision$</strong> of this document.
</p>
<p><b>Diagrams:</b></p>
<ul>
@@ -62,10 +62,11 @@
<p><b>Fundamental tables</b></p>
<ul>
<li><a href="#assembly">assembly</a></li>
- <li><a href="#chromosome">chromosome</a></li>
- <li><a href="#clone">clone</a></li>
- <li><a href="#contig">contig</a></li>
+ <li><a href="#assembly_exception">assembly_exception</a></li>
+ <li><a href="#attrib_type">attrib_type</a></li>
+ <li><a href="#coord_system">coord_system</a></li>
<li><a href="#dna">dna</a></li>
+ <li><a href="#dnac">dnac</a></li>
<li><a href="#exon">exon</a></li>
<li><a href="#exon_stable_id">exon_stable_id</a></li>
<li><a href="#exon_transcript">exon_transcript</a></li>
@@ -74,6 +75,11 @@
<li><a href="#gene_stable_id">gene_stable_id</a></li>
<li><a href="#karyotype">karyotype</a></li>
<li><a href="#meta">meta</a></li>
+ <li><a href="#meta_coord">meta_coord</a></li>
+ <li><a href="#prediction_exon">prediction_exon</a></li>
+ <li><a href="#prediction_transcript">prediction_transcript</a></li>
+ <li><a href="#seq_region">seq_region</a></li>
+ <li><a href="#seq_region_attrib">seq_region_attrib</a></li>
<li><a href="#supporting_feature">supporting_feature</a></li>
<li><a href="#transcript">transcript</a></li>
<li><a href="#transcript_stable_id">transcript_stable_id</a></li>
@@ -84,10 +90,15 @@
<ul>
<li><a href="#analysis">analysis</a></li>
<li><a href="#dna_align_feature">dna_align_feature</a></li>
+ <li><a href="#map">map</a></li>
<li><a href="#marker">marker</a></li>
<li><a href="#marker_feature">marker_feature</a></li>
<li><a href="#marker_map_location">marker_map_location</a></li>
<li><a href="#marker_synonym">marker_synonym</a></li>
+ <li><a href="#misc_attrib">misc_attrib</a></li>
+ <li><a href="#misc_feature">misc_feature</a></li>
+ <li><a href="#misc_feature_misc_set">misc_feature_misc_set</a></li>
+ <li><a href="#misc_set">misc_set</a></li>
<li><a href="#prediction_transcript">prediction_transcript</a></li>
<li><a href="#protein_align_feature">protein_align_feature</a></li>
<li><a href="#protein_feature">protein_feature</a></li>
@@ -134,28 +145,63 @@
- <h3><a name="clone">clone</a></h3>
- <p>Contains information about BAC clones inside the EnsEMBL database. As this was the way that DNA came into EnsEMBL for the
- human genome, all DNA has to be specified in terms of BAC clones, even if they don't exist. Also, some of the BAC clones in
- early versions of the EnsEMBL database haven't been submitted to EMBL but were Sanger Institute internal BAC names. This is
- why clone has two different possible identifiers (and versions for each of them). Note that although there are many dates
- inside this table, they are not well maintained. The htg_phase column describes whether High Throughput Genomic sequencing
- is finished or unfinished: draft is 123, finished is 4.
+ <h3><a name="seq_region">seq_region</a></h3>
+ <p>Stores information about sequence regions. The primary key is used as a pointer into the dna table so that actual sequence
+ can be obtained, and the coord_system_id allows sequence regions of multiple types to be stored. Clones, contigs and chromosomes
+ are all now stored in the seq_region table. Contigs are stored with the co-ordinate system 'contig'. The relationship between
+ contigs and clones is stored in the assembly table. The relationships between contigs and chromosomes, and between contigs
+ and supercontigs, are stored in the assembly table.
</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#dna">dna</a> - 1:1 relationship to the dna table.
+ </li>
+ <li><a href="#coord_system">coord_system</a> - Describes which co-ordinates a particular feature is stored in.
+ </li>
+ </ul>
+
+
+
+
+ <h3><a name="coord_system">coord_system</a></h3>
+ <p>Stores information about the available co-ordinate systems for this species. Note that there must be one co-ordinate system
+ that has the attribute "top_level" and one that has the attribute "sequence_level".
+ </p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#seq_region">seq_region</a> - Has coord_system_id foreign key to allow joins with the coord_system table.
+ </li>
+ </ul>
- <h3><a name="contig">contig</a></h3>
- <p>A contig is a piece of DNA inside a clone that is contiguous i.e. not interupted by sequencing gaps. One or more contigs made
- up the BAC clone sequence. Due to this historical importance, they are currently the reference coordinate system for all features
- inside EnsEMBL, although not all species come in clones/contigs. Contigs directly link to dna table entries which contain
- the actual sequence information. Currently, fake clone and contig entries must be faked for genomes that don't have these
- things. The sequence is that of the contig, not that of the golden path, i.e. to construct the golden path from the dna entries,
- the sequence of contigs with an orientation of -1 must be reversed and bases complemented. The assembly table has the contig
- orientation (raw_ori). Note the length of the dna.sequence field is always equal to the appropriate length field in the contig
- table.
+ <h3><a name="seq_region_attrib">seq_region_attrib</a></h3>
+ <p>Allows "attributes" to be defined for certain seq_regions. Provides a way of storing extra information about particular seq_regions
+ without adding extra columns to the seq_region table. e.g.
</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#seq_region">seq_region</a> -
+ </li>
+ <li><a href="#attrib_type">attrib_type</a> - Provides codes, names and desctriptions of attribute types.
+ </li>
+ </ul>
+
+
+
+
+ <h3><a name="attrib_type">attrib_type</a></h3>
+ <p>Provides codes, names and desctriptions of attribute types.</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#seq_region_attrib">seq_region_attrib</a> - Associates seq_regions with attributes.
+ </li>
+ </ul>
@@ -165,7 +211,7 @@
<p><b>See also:</b></p>
<p>Tables:</p>
<ul>
- <li><a href="#contig">contig</a> - 1:1 relationship to the dna table.
+ <li><a href="#seq_region">seq_region</a> - Relates sequence to features.
</li>
<li><a href="#external_synonym">external_synonym</a> - Allows xrefs to have more than one name
</li>
@@ -174,6 +220,12 @@
+ <h3><a name="dnac">dnac</a></h3>
+ <p>Stores compressed DNA sequence.</p>
+
+
+
+
<h3><a name="assembly">assembly</a></h3>
<p>Describes how contig sequences make up the chromosomal sequence. The data in this table defines the "static golden path",
i.e. the best effort draft full genome sequence as determined by the UCSC or NCBI (depending which assembly you are using)
@@ -186,14 +238,11 @@
<p><b>See also:</b></p>
<p>Tables:</p>
<ul>
- <li><a href="#contig">contig</a> -
+ <li><a href="#seq_region">seq_region</a> - Stores extra information about both the assembled object and its component parts
</li>
</ul>
<p>Concepts:</p>
<ul>
- <li><a href="#co-ordinates">co-ordinates</a> - Chromosome start and end positions are stored in chromosomal co-ordinates whereas the contig start and end positions are stored
- in contig co-ordinates.
- </li>
<li><a href="#supercontigs">supercontigs</a> - The mapping between contigs and supercontigs is also stored in the assembly table.
</li>
</ul>
@@ -201,10 +250,17 @@
- <h3><a name="chromosome">chromosome</a></h3>
- <p>Describes chromosomes. Currently contains the name and length of each chromosome for the species. Currently also contains
- additional rows to allow genes to be stored even if the chromosome on which the gene appears is not positively identified.
+ <h3><a name="assembly_exception">assembly_exception</a></h3>
+ <p>Allows multiple sequence regions to point to the same sequence, analogous to a symbolic link in a filesystem pointing to the
+ actual file. This mechanism has been implemented specifically to support haplotypes and PARs, but may be useful for other
+ similar structures in the future.
</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#assembly">assembly</a> -
+ </li>
+ </ul>
@@ -216,21 +272,13 @@
<h3><a name="exon">exon</a></h3>
- <p>Stores data about exons. Associated with transcripts via exon_transcript. Allows access to contigs via the contig_* columns.
- The sticky_rank differentiates between fragments of the same exon; i.e for exons that span multiple contigs, all the fragments
- are in this table with the same id, but different sticky_rank values.
- </p>
+ <p>Stores data about exons. Associated with transcripts via exon_transcript. Allows access to contigsseq_regions.</p>
<p><b>See also:</b></p>
<p>Tables:</p>
<ul>
<li><a href="#exon_transcript">exon_transcript</a> - Used to associate exons with transcripts.
</li>
</ul>
- <p>Concepts:</p>
- <ul>
- <li><a href="#sticky_rank">sticky_rank</a> - Differentiates between exons that span multiple contigs.
- </li>
- </ul>
@@ -249,8 +297,9 @@
<h3><a name="transcript">transcript</a></h3>
- <p>TBC Note that a transcript is usually associated with a translation, but may not be, e.g. in the case of pseudogenes and RNA
- genes (those that code for RNA molecules).
+ <p>Stores information about transcripts. Has seq_region_start, seq_region_end and seq_region_strand for faster retrieval and
+ to allow storage independently of genes and exons. Note that a transcript is usually associated with a translation, but may
+ not be, e.g. in the case of pseudogenes and RNA genes (those that code for RNA molecules).
</p>
@@ -321,7 +370,7 @@
<h3><a name="translation">translation</a></h3>
<p>Describes which parts of which exons are used in translation. The seq_start and seq_end columns are 1-based offsets into the
*relative* coordinate system of start_exon_id and end_exon_id. i.e, if the translation starts at the first base of the exon,
- seq_start would be 1.
+ seq_start would be 1. Transcripts are related to translations by the transcript_id key in this table.
</p>
@@ -349,9 +398,24 @@
+ <h3><a name="prediction_transcript">prediction_transcript</a></h3>
+ <p>Stores transcripts that are predicted by ab initio gene finder programs (e.g. genscan, SNAP). Unlike EnsEMBL transcripts they
+ are not supported by any evidence.
+ </p>
+
+
+
+
+ <h3><a name="prediction_exon">prediction_exon</a></h3>
+ <p>Stores exons that are predicted by ab initio gene finder programs. Unlike EnsEMBL exons they are not supported by any evidence.</p>
+
+
+
+
<h3><a name="meta">meta</a></h3>
<p>Stores data about the data in the current schema. Taxonomy information, version information and the default value for the
type column in the assembly table are stored here. Unlike other tables, data in the meta table is stored as key/value pairs.
+ Also stores (via assembly.mapping keys) the relationships between co-ordinate systms in the assembly table.
</p>
<p><b>See also:</b></p>
<p>Tables:</p>
@@ -361,6 +425,19 @@
</ul>
+
+
+
+ <h3><a name="meta_coord">meta_coord</a></h3>
+ <p>Describes which co-ordinate systems the different feature tables use.</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#coord_system">coord_system</a> -
+ </li>
+ </ul>
+
+
<hr>
<h2>Features and analyses</h2>
<p></p>
@@ -507,12 +584,16 @@
<h3><a name="marker_map_location">marker_map_location</a></h3>
- <p>Allows storage of information about the postion of a marker.</p>
+ <p>Allows storage of information about the postion of a marker - these are positions on genetic or radiation hybrid maps (as
+ opposed to positions on the assembly, which EnsEMBL has determined and which are stored in marker_feature).
+ </p>
<p><b>See also:</b></p>
<p>Tables:</p>
<ul>
<li><a href="#marker">marker</a> - Stores marker data.
</li>
+ <li><a href="#marker_feature">marker_feature</a> - Stores marker positions on the assembly.
+ </li>
</ul>
@@ -530,10 +611,73 @@
+ <h3><a name="map">map</a></h3>
+ <p>Stores the names of different genetic or radiation hybrid maps, for which there is marker map information.</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#marker">marker</a> - Stores the original marker.
+ </li>
+ </ul>
+
+
+
+
<h3><a name="repeat_consensus">repeat_consensus</a></h3>
<p>Stores consenus sequences obtained from analysing repeat features.</p>
+
+
+ <h3><a name="misc_feature">misc_feature</a></h3>
+ <p>Alllows for storage of arbitrary features.</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#misc_attrib">misc_attrib</a> - Allows storage of arbitrary attributes for the misc_features.
+ </li>
+ </ul>
+
+
+
+
+ <h3><a name="misc_attrib">misc_attrib</a></h3>
+ <p>Stores arbitrary attributes about the features in the misc_feature table.</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#misc_feature">misc_feature</a> -
+ </li>
+ </ul>
+
+
+
+
+ <h3><a name="misc_set">misc_set</a></h3>
+ <p>Defines "sets" that the features held in the misc_feature table can be grouped into.</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#misc_feature_misc_set">misc_feature_misc_set</a> - Defines which features are in which set
+ </li>
+ </ul>
+
+
+
+
+ <h3><a name="misc_feature_misc_set">misc_feature_misc_set</a></h3>
+ <p>Defines which of the features in misc_feature are in which of the sets defined in misc_set</p>
+ <p><b>See also:</b></p>
+ <p>Tables:</p>
+ <ul>
+ <li><a href="#misc_feature">misc_feature</a> -
+ </li>
+ <li><a href="#misc_set">misc_set</a> -
+ </li>
+ </ul>
+
+
+
<hr>
<h2>ID Mapping</h2>
<p>Tables involved in mapping identifiers between releases</p>
@@ -712,7 +856,8 @@
position it is relative to. CONTIG co-ordinates, also called 'raw contig' co-ordinates or 'clone fragments' are relative to
the first base of the first contig of a clone. Note that the numbering is from 1, i.e. the very first base of the first contig
of a clone is numbered 1, not 0. In CHROMOSOMAL co-ordinates, the co-ordinates are relative to the first base of the chromosome.
- Again, numbering is from 1.
+ Again, numbering is from 1. The seq_region table can store sequence regions in any of the co-ordinate systems defined in the
+ coord_system table.
</p>
</dd>
<dt>
diff --git a/docs/schema_description/tables.txt b/docs/schema_description/tables.txt
index de4fc3db16..8272c32823 100644
--- a/docs/schema_description/tables.txt
+++ b/docs/schema_description/tables.txt
@@ -15,7 +15,7 @@ This document gives a high-level description of the tables that make up the EnsE
||4a||Analyze genes - populate protein_feature, xref tables, interpro||
||4b||ID mapping||
-This document refers to version ''15'' of the EnsEMBL core schema. You are looking at revision ''1.21''of this document.
+This document refers to version ''15'' of the EnsEMBL core schema. You are looking at revision ''$Revision$''of this document.
'''Diagrams:'''
@@ -30,10 +30,11 @@ This document refers to version ''15'' of the EnsEMBL core schema. You are looki
'''Fundamental tables'''
* assembly
- * chromosome
- * clone
- * contig
+ * assembly_exception
+ * attrib_type
+ * coord_system
* dna
+ * dnac
* exon
* exon_stable_id
* exon_transcript
@@ -42,6 +43,11 @@ This document refers to version ''15'' of the EnsEMBL core schema. You are looki
* gene_stable_id
* karyotype
* meta
+ * meta_coord
+ * prediction_exon
+ * prediction_transcript
+ * seq_region
+ * seq_region_attrib
* supporting_feature
* transcript
* transcript_stable_id
@@ -52,10 +58,15 @@ This document refers to version ''15'' of the EnsEMBL core schema. You are looki
* analysis
* dna_align_feature
+ * map
* marker
* marker_feature
* marker_map_location
* marker_synonym
+ * misc_attrib
+ * misc_feature
+ * misc_feature_misc_set
+ * misc_set
* prediction_transcript
* protein_align_feature
* protein_feature
@@ -98,14 +109,52 @@ This document refers to version ''15'' of the EnsEMBL core schema. You are looki
-===clone===
-Contains information about BAC clones inside the EnsEMBL database. As this was the way that DNA came into EnsEMBL for the human genome, all DNA has to be specified in terms of BAC clones, even if they don't exist. Also, some of the BAC clones in early versions of the EnsEMBL database haven't been submitted to EMBL but were Sanger Institute internal BAC names. This is why clone has two different possible identifiers (and versions for each of them). Note that although there are many dates inside this table, they are not well maintained. The htg_phase column describes whether High Throughput Genomic sequencing is finished or unfinished: draft is 123, finished is 4.
+===seq_region===
+Stores information about sequence regions. The primary key is used as a pointer into the dna table so that actual sequence can be obtained, and the coord_system_id allows sequence regions of multiple types to be stored. Clones, contigs and chromosomes are all now stored in the seq_region table. Contigs are stored with the co-ordinate system 'contig'. The relationship between contigs and clones is stored in the assembly table. The relationships between contigs and chromosomes, and between contigs and supercontigs, are stored in the assembly table.
+'''See also:'''
+
+
+Tables:
+
+ * dna - 1:1 relationship to the dna table.
+ * coord_system - Describes which co-ordinates a particular feature is stored in.
+
+
+
+
+===coord_system===
+Stores information about the available co-ordinate systems for this species. Note that there must be one co-ordinate system that has the attribute "top_level" and one that has the attribute "sequence_level".
+'''See also:'''
+
+
+Tables:
+ * seq_region - Has coord_system_id foreign key to allow joins with the coord_system table.
+
-===contig===
-A contig is a piece of DNA inside a clone that is contiguous i.e. not interupted by sequencing gaps. One or more contigs made up the BAC clone sequence. Due to this historical importance, they are currently the reference coordinate system for all features inside EnsEMBL, although not all species come in clones/contigs. Contigs directly link to dna table entries which contain the actual sequence information. Currently, fake clone and contig entries must be faked for genomes that don't have these things. The sequence is that of the contig, not that of the golden path, i.e. to construct the golden path from the dna entries, the sequence of contigs with an orientation of -1 must be reversed and bases complemented. The assembly table has the contig orientation (raw_ori). Note the length of the dna.sequence field is always equal to the appropriate length field in the contig table.
+===seq_region_attrib===
+Allows "attributes" to be defined for certain seq_regions. Provides a way of storing extra information about particular seq_regions without adding extra columns to the seq_region table. e.g.
+'''See also:'''
+
+
+Tables:
+
+ * seq_region -
+ * attrib_type - Provides codes, names and desctriptions of attribute types.
+
+
+
+
+===attrib_type===
+Provides codes, names and desctriptions of attribute types.
+'''See also:'''
+
+
+Tables:
+
+ * seq_region_attrib - Associates seq_regions with attributes.
@@ -117,8 +166,14 @@ Contains DNA sequence. This table has a 1:1 relationship with the contig table.
Tables:
- * contig - 1:1 relationship to the dna table.
+ * seq_region - Relates sequence to features.
* external_synonym - Allows xrefs to have more than one name
+
+
+
+
+===dnac===
+Stores compressed DNA sequence.
@@ -130,20 +185,25 @@ Describes how contig sequences make up the chromosomal sequence. The data in thi
Tables:
- * contig -
+ * seq_region - Stores extra information about both the assembled object and its component parts
Concepts:
- * co-ordinates - Chromosome start and end positions are stored in chromosomal co-ordinates whereas the contig start and end positions are stored in contig co-ordinates.
* supercontigs - The mapping between contigs and supercontigs is also stored in the assembly table.
-===chromosome===
-Describes chromosomes. Currently contains the name and length of each chromosome for the species. Currently also contains additional rows to allow genes to be stored even if the chromosome on which the gene appears is not positively identified.
+===assembly_exception===
+Allows multiple sequence regions to point to the same sequence, analogous to a symbolic link in a filesystem pointing to the actual file. This mechanism has been implemented specifically to support haplotypes and PARs, but may be useful for other similar structures in the future.
+'''See also:'''
-
+
+Tables:
+
+ * assembly -
+
+
===karyotype===
@@ -153,7 +213,7 @@ Describes bands that can be stained on the chromosome.
===exon===
-Stores data about exons. Associated with transcripts via exon_transcript. Allows access to contigs via the contig_* columns. The sticky_rank differentiates between fragments of the same exon; i.e for exons that span multiple contigs, all the fragments are in this table with the same id, but different sticky_rank values.
+Stores data about exons. Associated with transcripts via exon_transcript. Allows access to contigsseq_regions.
'''See also:'''
@@ -161,10 +221,6 @@ Tables:
* exon_transcript - Used to associate exons with transcripts.
-Concepts:
-
- * sticky_rank - Differentiates between exons that span multiple contigs.
-
@@ -182,7 +238,7 @@ Concepts:
===transcript===
-TBC Note that a transcript is usually associated with a translation, but may not be, e.g. in the case of pseudogenes and RNA genes (those that code for RNA molecules).
+Stores information about transcripts. Has seq_region_start, seq_region_end and seq_region_strand for faster retrieval and to allow storage independently of genes and exons. Note that a transcript is usually associated with a translation, but may not be, e.g. in the case of pseudogenes and RNA genes (those that code for RNA molecules).
@@ -247,7 +303,7 @@ Tables:
===translation===
-Describes which parts of which exons are used in translation. The seq_start and seq_end columns are 1-based offsets into the *relative* coordinate system of start_exon_id and end_exon_id. i.e, if the translation starts at the first base of the exon, seq_start would be 1.
+Describes which parts of which exons are used in translation. The seq_start and seq_end columns are 1-based offsets into the *relative* coordinate system of start_exon_id and end_exon_id. i.e, if the translation starts at the first base of the exon, seq_start would be 1. Transcripts are related to translations by the transcript_id key in this table.
@@ -272,8 +328,20 @@ Describes the exon prediction process by linking exons to DNA or protein alignme
+===prediction_transcript===
+Stores transcripts that are predicted by ab initio gene finder programs (e.g. genscan, SNAP). Unlike EnsEMBL transcripts they are not supported by any evidence.
+
+
+
+
+===prediction_exon===
+Stores exons that are predicted by ab initio gene finder programs. Unlike EnsEMBL exons they are not supported by any evidence.
+
+
+
+
===meta===
-Stores data about the data in the current schema. Taxonomy information, version information and the default value for the type column in the assembly table are stored here. Unlike other tables, data in the meta table is stored as key/value pairs.
+Stores data about the data in the current schema. Taxonomy information, version information and the default value for the type column in the assembly table are stored here. Unlike other tables, data in the meta table is stored as key/value pairs. Also stores (via assembly.mapping keys) the relationships between co-ordinate systms in the assembly table.
'''See also:'''
@@ -281,6 +349,19 @@ Tables:
* assembly - The default value for assembly.type is stored in the meta table.
+
+
+
+
+===meta_coord===
+Describes which co-ordinate systems the different feature tables use.
+'''See also:'''
+
+
+Tables:
+
+ * coord_system -
+
----
== Features and analyses ==
@@ -412,13 +493,14 @@ Tables:
===marker_map_location===
-Allows storage of information about the postion of a marker.
+Allows storage of information about the postion of a marker - these are positions on genetic or radiation hybrid maps (as opposed to positions on the assembly, which EnsEMBL has determined and which are stored in marker_feature).
'''See also:'''
Tables:
* marker - Stores marker data.
+ * marker_feature - Stores marker positions on the assembly.
@@ -435,9 +517,71 @@ Tables:
+===map===
+Stores the names of different genetic or radiation hybrid maps, for which there is marker map information.
+'''See also:'''
+
+
+Tables:
+
+ * marker - Stores the original marker.
+
+
+
+
===repeat_consensus===
Stores consenus sequences obtained from analysing repeat features.
+
+
+
+===misc_feature===
+Alllows for storage of arbitrary features.
+'''See also:'''
+
+
+Tables:
+
+ * misc_attrib - Allows storage of arbitrary attributes for the misc_features.
+
+
+
+
+===misc_attrib===
+Stores arbitrary attributes about the features in the misc_feature table.
+'''See also:'''
+
+
+Tables:
+
+ * misc_feature -
+
+
+
+
+===misc_set===
+Defines "sets" that the features held in the misc_feature table can be grouped into.
+'''See also:'''
+
+
+Tables:
+
+ * misc_feature_misc_set - Defines which features are in which set
+
+
+
+
+===misc_feature_misc_set===
+Defines which of the features in misc_feature are in which of the sets defined in misc_set
+'''See also:'''
+
+
+Tables:
+
+ * misc_feature -
+ * misc_set -
+
+
----
== ID Mapping ==
@@ -588,7 +732,7 @@ Links:
==Concepts==
''co-ordinates ''
-: There are several different co-ordinate systems used in the EnsEMBL database and API. For every co-ordinate system, the fundamental unit is one base. The differences between co-ordinate systems lie in where a particular numbered base lies, and the start position it is relative to. CONTIG co-ordinates, also called 'raw contig' co-ordinates or 'clone fragments' are relative to the first base of the first contig of a clone. Note that the numbering is from 1, i.e. the very first base of the first contig of a clone is numbered 1, not 0. In CHROMOSOMAL co-ordinates, the co-ordinates are relative to the first base of the chromosome. Again, numbering is from 1.
+: There are several different co-ordinate systems used in the EnsEMBL database and API. For every co-ordinate system, the fundamental unit is one base. The differences between co-ordinate systems lie in where a particular numbered base lies, and the start position it is relative to. CONTIG co-ordinates, also called 'raw contig' co-ordinates or 'clone fragments' are relative to the first base of the first contig of a clone. Note that the numbering is from 1, i.e. the very first base of the first contig of a clone is numbered 1, not 0. In CHROMOSOMAL co-ordinates, the co-ordinates are relative to the first base of the chromosome. Again, numbering is from 1. The seq_region table can store sequence regions in any of the co-ordinate systems defined in the coord_system table.
''supercontigs ''
: A supercontig is made up of a group of adjacent or overlapping contigs.
''sticky_rank ''
diff --git a/docs/schema_description/tables.xml b/docs/schema_description/tables.xml
index 0e70d4a488..7b928e1c6d 100644
--- a/docs/schema_description/tables.xml
+++ b/docs/schema_description/tables.xml
@@ -25,29 +25,67 @@
<!-- ============================================================ -->
- <table>
+ <table>
- <name>clone</name>
+ <name>seq_region</name>
- <description>Contains information about BAC clones inside the EnsEMBL database. As this was the way that DNA came into EnsEMBL for the human genome, all DNA has to be specified in terms of BAC clones, even if they don't exist. Also, some of the BAC clones in early versions of the EnsEMBL database haven't been submitted to EMBL but were Sanger Institute internal BAC names. This is why clone has two different possible identifiers (and versions for each of them). Note that although there are many dates inside this table, they are not well maintained. The htg_phase column describes whether High Throughput Genomic sequencing is finished or unfinished: draft is 123, finished is 4. </description>
+ <description>Stores information about sequence regions. The primary key is used as a pointer into the dna table so that actual sequence can be obtained, and the coord_system_id allows sequence regions of multiple types to be stored. Clones, contigs and chromosomes are all now stored in the seq_region table. Contigs are stored with the co-ordinate system 'contig'. The relationship between contigs and clones is stored in the assembly table. The relationships between contigs and chromosomes, and between contigs and supercontigs, are stored in the assembly table. </description>
<used></used>
-
- <see></see>
+
+ <see>
+ <tableref name="dna" reason="1:1 relationship to the dna table."/>
+ <tableref name="coord_system" reason="Describes which co-ordinates a particular feature is stored in."/>
+ </see>
</table>
<!-- ============================================================ -->
- <table>
+ <table>
- <name>contig</name>
+ <name>coord_system</name>
- <description>A contig is a piece of DNA inside a clone that is contiguous i.e. not interupted by sequencing gaps. One or more contigs made up the BAC clone sequence. Due to this historical importance, they are currently the reference coordinate system for all features inside EnsEMBL, although not all species come in clones/contigs. Contigs directly link to dna table entries which contain the actual sequence information. Currently, fake clone and contig entries must be faked for genomes that don't have these things. The sequence is that of the contig, not that of the golden path, i.e. to construct the golden path from the dna entries, the sequence of contigs with an orientation of -1 must be reversed and bases complemented. The assembly table has the contig orientation (raw_ori). Note the length of the dna.sequence field is always equal to the appropriate length field in the contig table.</description>
+ <description>Stores information about the available co-ordinate systems for this species. Note that there must be one co-ordinate system that has the attribute "top_level" and one that has the attribute "sequence_level".</description>
<used></used>
+
+ <see>
+ <tableref name="seq_region" reason="Has coord_system_id foreign key to allow joins with the coord_system table."/>
+ </see>
- <see></see>
+ </table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>seq_region_attrib</name>
+
+ <description>Allows "attributes" to be defined for certain seq_regions. Provides a way of storing extra information about particular seq_regions without adding extra columns to the seq_region table. e.g. </description>
+
+ <used></used>
+
+ <see>
+ <tableref name="seq_region" reason=""/>
+ <tableref name="attrib_type" reason="Provides codes, names and desctriptions of attribute types."/>
+ </see>
+
+ </table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>attrib_type</name>
+
+ <description>Provides codes, names and desctriptions of attribute types.</description>
+
+ <used></used>
+
+ <see>
+ <tableref name="seq_region_attrib" reason="Associates seq_regions with attributes."/>
+ </see>
</table>
@@ -62,11 +100,27 @@
<used></used>
<see>
- <tableref name="contig" reason="1:1 relationship to the dna table."/>
+ <tableref name="seq_region" reason="Relates sequence to features."/>
<tableref name="external_synonym" reason="Allows xrefs to have more than one name"/>
</see>
</table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>dnac</name>
+
+ <description>Stores compressed DNA sequence.</description>
+
+ <used></used>
+
+ <see>
+
+ </see>
+
+ </table>
<!-- ============================================================ -->
@@ -79,8 +133,7 @@
<used></used>
<see>
- <tableref name="contig" reason=""/>
- <conceptref name="co-ordinates" reason="Chromosome start and end positions are stored in chromosomal co-ordinates whereas the contig start and end positions are stored in contig co-ordinates."/>
+ <tableref name="seq_region" reason="Stores extra information about both the assembled object and its component parts"/>
<conceptref name="supercontigs" reason="The mapping between contigs and supercontigs is also stored in the assembly table."/>
</see>
@@ -90,19 +143,19 @@
<table>
- <name>chromosome</name>
+ <name>assembly_exception</name>
- <description>Describes chromosomes. Currently contains the name and length of each chromosome for the species. Currently also contains additional rows to allow genes to be stored even if the chromosome on which the gene appears is not positively identified.</description>
+ <description>Allows multiple sequence regions to point to the same sequence, analogous to a symbolic link in a filesystem pointing to the actual file. This mechanism has been implemented specifically to support haplotypes and PARs, but may be useful for other similar structures in the future.</description>
<used></used>
<see>
-
+ <tableref name="assembly" reason=""/>
</see>
</table>
- <!-- ============================================================ -->
+ <!-- ============================================================ -->
<table>
@@ -124,13 +177,12 @@
<name>exon</name>
- <description>Stores data about exons. Associated with transcripts via exon_transcript. Allows access to contigs via the contig_* columns. The sticky_rank differentiates between fragments of the same exon; i.e for exons that span multiple contigs, all the fragments are in this table with the same id, but different sticky_rank values.</description>
+ <description>Stores data about exons. Associated with transcripts via exon_transcript. Allows access to contigsseq_regions.</description>
<used></used>
<see>
<tableref name="exon_transcript" reason="Used to associate exons with transcripts."/>
- <conceptref name="sticky_rank" reason="Differentiates between exons that span multiple contigs."/>
</see>
</table>
@@ -158,7 +210,7 @@
<name>transcript</name>
- <description>TBC Note that a transcript is usually associated with a translation, but may not be, e.g. in the case of pseudogenes and RNA genes (those that code for RNA molecules).</description>
+ <description>Stores information about transcripts. Has seq_region_start, seq_region_end and seq_region_strand for faster retrieval and to allow storage independently of genes and exons. Note that a transcript is usually associated with a translation, but may not be, e.g. in the case of pseudogenes and RNA genes (those that code for RNA molecules).</description>
<used></used>
@@ -257,7 +309,7 @@
<name>translation</name>
- <description>Describes which parts of which exons are used in translation. The seq_start and seq_end columns are 1-based offsets into the *relative* coordinate system of start_exon_id and end_exon_id. i.e, if the translation starts at the first base of the exon, seq_start would be 1.</description>
+ <description>Describes which parts of which exons are used in translation. The seq_start and seq_end columns are 1-based offsets into the *relative* coordinate system of start_exon_id and end_exon_id. i.e, if the translation starts at the first base of the exon, seq_start would be 1. Transcripts are related to translations by the transcript_id key in this table.</description>
<used></used>
@@ -301,11 +353,41 @@
<!-- ============================================================ -->
+ <table>
+
+ <name>prediction_transcript</name>
+
+ <description>Stores transcripts that are predicted by ab initio gene finder programs (e.g. genscan, SNAP). Unlike EnsEMBL transcripts they are not supported by any evidence.</description>
+
+ <used></used>
+
+ <see>
+ </see>
+
+ </table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>prediction_exon</name>
+
+ <description>Stores exons that are predicted by ab initio gene finder programs. Unlike EnsEMBL exons they are not supported by any evidence.</description>
+
+ <used></used>
+
+ <see>
+ </see>
+
+ </table>
+
+ <!-- ============================================================ -->
+
<table>
<name>meta</name>
- <description>Stores data about the data in the current schema. Taxonomy information, version information and the default value for the type column in the assembly table are stored here. Unlike other tables, data in the meta table is stored as key/value pairs.</description>
+ <description>Stores data about the data in the current schema. Taxonomy information, version information and the default value for the type column in the assembly table are stored here. Unlike other tables, data in the meta table is stored as key/value pairs. Also stores (via assembly.mapping keys) the relationships between co-ordinate systms in the assembly table.</description>
<used></used>
@@ -315,6 +397,23 @@
</table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>meta_coord</name>
+
+ <description>Describes which co-ordinate systems the different feature tables use.</description>
+
+ <used></used>
+
+ <see>
+ <tableref name="coord_system" reason=""/>
+ </see>
+
+ </table>
+
</tablegroup>
<tablegroup name="Features and analyses" description="">
@@ -579,6 +678,72 @@
</table>
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>misc_feature</name>
+
+ <description>Alllows for storage of arbitrary features.</description>
+
+ <used></used>
+
+ <see>
+ <tableref name="misc_attrib" reason="Allows storage of arbitrary attributes for the misc_features."/>
+ </see>
+
+ </table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>misc_attrib</name>
+
+ <description>Stores arbitrary attributes about the features in the misc_feature table.</description>
+
+ <used></used>
+
+ <see>
+ <tableref name="misc_feature" reason=""/>
+ </see>
+
+ </table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>misc_set</name>
+
+ <description>Defines "sets" that the features held in the misc_feature table can be grouped into.</description>
+
+ <used></used>
+
+ <see>
+ <tableref name="misc_feature_misc_set" reason="Defines which features are in which set"/>
+ </see>
+
+ </table>
+
+ <!-- ============================================================ -->
+
+ <table>
+
+ <name>misc_feature_misc_set</name>
+
+ <description>Defines which of the features in misc_feature are in which of the sets defined in misc_set</description>
+
+ <used></used>
+
+ <see>
+ <tableref name="misc_feature" reason=""/>
+ <tableref name="misc_set" reason=""/>
+ </see>
+
+ </table>
+
+
</tablegroup> <!-- Features and analyses-->
<!-- ============================================================ -->
@@ -782,7 +947,7 @@
<concepts>
- <concept name="co-ordinates" description="There are several different co-ordinate systems used in the EnsEMBL database and API. For every co-ordinate system, the fundamental unit is one base. The differences between co-ordinate systems lie in where a particular numbered base lies, and the start position it is relative to. CONTIG co-ordinates, also called 'raw contig' co-ordinates or 'clone fragments' are relative to the first base of the first contig of a clone. Note that the numbering is from 1, i.e. the very first base of the first contig of a clone is numbered 1, not 0. In CHROMOSOMAL co-ordinates, the co-ordinates are relative to the first base of the chromosome. Again, numbering is from 1."/>
+ <concept name="co-ordinates" description="There are several different co-ordinate systems used in the EnsEMBL database and API. For every co-ordinate system, the fundamental unit is one base. The differences between co-ordinate systems lie in where a particular numbered base lies, and the start position it is relative to. CONTIG co-ordinates, also called 'raw contig' co-ordinates or 'clone fragments' are relative to the first base of the first contig of a clone. Note that the numbering is from 1, i.e. the very first base of the first contig of a clone is numbered 1, not 0. In CHROMOSOMAL co-ordinates, the co-ordinates are relative to the first base of the chromosome. Again, numbering is from 1. The seq_region table can store sequence regions in any of the co-ordinate systems defined in the coord_system table."/>
<!-- TODO a diagram for all this -->
<concept name="supercontigs" description="A supercontig is made up of a group of adjacent or overlapping contigs."/>
diff --git a/docs/tutorial/ensembl_tutorial.doc b/docs/tutorial/ensembl_tutorial.doc
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diff --git a/docs/tutorial/ensembl_tutorial.pdf b/docs/tutorial/ensembl_tutorial.pdf
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+systemdict /languagelevel known not {
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+} def
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-[{
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-[{
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-[{
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-[{
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- <</Duplex false /Tumble false>> setpagedevice
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-[{
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-[{
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-[{
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-[{
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-[{
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-[{
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-[{
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-[{
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-globaldict /ColorSmartColorMatching known {
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-[{
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- << /CMYKInkSet 0 >> setpagedevice
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-dup 44 /r put
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-dup 51 /colon put
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-109 1568 m
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-109 1768 m
-(F1H6\(%/M)[ 27.347002 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 ] xS
-109 1818 m
-(7L6\(1/+\)#0+6+!+3\(3#Q#6'\(#T\(/\(1+\)#%0\(+M)[ 27.347002 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 22.837000 9.143001 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 ] xS
-109 1868 m
-(KYF3M)[ 27.347002 29.643002 27.347002 20.500002 11.398002 ] xS
-109 1918 m
-($+1?\(1I\(+651\(3M)[ 34.194004 22.837000 13.694001 20.500002 22.837000 13.694001 25.051003 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 ] xS
-109 1968 m
-(<#W%/+\):#!56#%,-H16+/6:#/H6\(#6H#53\(13#HW#H\)0\(1#2\(13%H/3#HW#6'\(#7/37$9O#<FG#Q#O%363#+/0#O%36#U\(W\(1\(/&\(3)[ 27.347002 11.398002 11.398002 9.143001 22.837000 22.837000 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 9.143001 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 13.694001 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 9.143001 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 20.500002 ] xS
-/F1.1[ 50 0 0 -50 0 0]sf
-181 2076 m
-(44444444444444444444444444444444444444444444444444444444444444444444444)[ 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 27.850002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2177 m
-(6"*,+7\)8*-+")[ 11.398002 25.051003 13.694001 15.990001 25.051003 25.051003 25.051003 22.837000 13.694001 11.398002 25.051003 25.051003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2291 m
-(G6#%3#%,-H16+/6#6H#53#6'+6#"H5#3'H5\)0#!\(#+!\)\(#6H#+&&\(33#6'\(#0+6+#%/#7/37$9O#0+6+!+3\(3#+3#\(+3%\)"#+3#-H33%!\)\(M##XH#61"#+/0)[ 11.398002 11.398002 11.398002 9.143001 20.500002 11.398002 9.143001 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 9.143001 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 9.143001 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 11.398002 13.694001 20.500002 11.398002 22.837000 22.837000 22.837000 ] xS
-48 2341 m
-(,\(\(6#6'\(#0%WW\(1\(/6#/\(\(03#HW#2+36\)"#0%WW\(1%/T#0+6+#53\(13#8\(#'+2\(#0\(2\(\)H-\(0#+#/5,!\(1#HW#%/6\(1W+&\(3#6H#6'\(#7/37$9O#0+6+M)[ 34.194004 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 13.694001 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 ] xS
-48 2391 m
-(ZH5#+1\(#-1H!+!\)"#1\(+0%/T#6'%3#0H&5,\(/6#!\(&+53\(#6'\(#8\(!#%/6\(1W+&\(:#8'%\)\(#!\(%/T#2\(1"#-1\(66"#+/0#W+36:#%3#-51\(\)"#+#8\(!)[ 27.347002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 9.143001 20.500002 11.398002 13.694001 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 29.643002 22.837000 9.143001 9.143001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 13.694001 22.837000 9.143001 20.500002 11.398002 22.837000 11.398002 29.643002 22.837000 22.837000 ] xS
-48 2441 m
-(%/6\(1W+&\(#+/0#8H/S6#-1H2%0\(#6'\(#?%/0#HW#!5\)?#%/WH1,+6%H/#"H5#+1\(#/\(\(0%/TM##GW#"H5#+1\(#+#!%H%/WH1,+6%&%+/#8'H#%3#F\(1\)#+8+1\(:)[ 9.143001 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 22.837000 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 20.500002 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 27.347002 22.837000 13.694001 9.143001 11.398002 22.837000 29.643002 22.837000 13.694001 22.837000 11.398002 ] xS
-48 2491 m
-(6'\(#<FG#6'+6#6'%3#0H&5,\(/6#0\(3&1%!\(3#%3#TH%/T#6H#!\(#2\(1"#53\(W5\)M##YH6#H/\)"#&+/#"H5#1\(61%\(2\(#0+6+#0%1\(&6\)"#6'1H5T'#"H51)[ 11.398002 22.837000 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 20.500002 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 ] xS
-48 2541 m
-(3&1%-63:#6H#\(%6'\(1#1\(,H6\(#H1#\)H&+\)#7/37$9O#0+6+!+3\(3:#"H5#&+/#0H#V5%6\(#+#\)H6#HW#0+6+#-1H&\(33%/T#2%+#6'\(#6HH\)3#!5%\)6#%/6H#6'\()[ 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 22.837000 22.837000 13.694001 11.398002 13.694001 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 22.837000 13.694001 11.398002 9.143001 22.837000 20.500002 22.837000 9.143001 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 11.398002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 9.143001 11.398002 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 2591 m
-(<FG#0+6+#6"-\(3M##[H8\(2\(1:#+#-H%/6#6'+6#/\(\(03#6H#!\(#,+0\(#/H8#%3#6'+6#"H5#,H36#-1H!+!\)"#;AYSX#'+2\(#6H#53\(#6'%3#<FG#%W)[ 27.347002 27.347002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 29.643002 22.837000 20.500002 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 9.143001 20.500002 11.398002 29.643002 31.898003 29.643002 7.831001 25.051003 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 27.347002 27.347002 11.398002 11.398002 9.143001 11.398002 ] xS
-48 2641 m
-("H51#0+6+#/\(\(03#+1\(#1\(\)+6%2\(\)"#3%,-\)\(M##G/36\(+0:#"H5#&+/#53\(#6'\(#-51-H3\(Q!5%\)6#0+6+Q,%/%/T#6HH\)#&+\)\)\(0#7/37$9O#$+16M##N\()[ 20.500002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 9.143001 22.837000 11.398002 9.143001 20.500002 22.837000 9.143001 20.500002 11.398002 20.500002 9.143001 34.194004 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 34.194004 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 34.194004 22.837000 13.694001 11.398002 11.398002 11.398002 11.398002 38.704006 22.837000 ] xS
-48 2691 m
-(0H/S6#8+/6#6H#&'+3\(#"H5#+8+":#!56#%W#"H5#'+2\(/S6#+\)1\(+0"#\)HH?\(0#+6#$+16#.888M\(/3\(,!\)MH1T47/3$+164@#8\(#\(/6'53%+36%&+\)\)")[ 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 29.643002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 9.143001 13.694001 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 34.194004 22.837000 13.694001 11.398002 11.398002 13.694001 29.643002 29.643002 29.643002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 11.398002 27.347002 22.837000 20.500002 34.194004 22.837000 13.694001 11.398002 11.398002 13.694001 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 20.500002 11.398002 9.143001 20.500002 22.837000 9.143001 9.143001 20.500002 ] xS
-48 2741 m
-(\(/&H51+T\(#"H5#6H#0H#3H#3HH/M)[ 22.837000 22.837000 20.500002 22.837000 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 ] xS
-48 2846 m
-(A?+"M##GW#"H5#+1\(#36%\)\)#'\(1\(:#THH0M##YH8#8\(#&+/#6+\)?#+!H56#+&&\(33%/T#7/37$9O#0+6+!+3\(3:#!H6'#0%1\(&6\)"#+/0#6'1H5T'#6'\()[ 31.898003 20.500002 22.837000 20.500002 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 11.398002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 29.643002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 2896 m
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-48 212 m
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-48 312 m
-(=##>\('?#'&-5"1#*/.2#.2%#1'.'#-"#@'@'A4'"$%&A'0A5@#*/.2#B54.#'#9?CD;#0\(/%".A)[ 15.990001 11.398002 11.398002 27.347002 9.143001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 34.194004 20.500002 27.347002 31.898003 22.837000 11.398002 20.500002 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 ] xS
-48 362 m
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-48 412 m
-(3/->%&\(#4-6.*'&%GA)[ 22.837000 9.143001 22.837000 27.347002 22.837000 13.694001 9.143001 11.398002 20.500002 22.837000 11.398002 11.398002 29.643002 22.837000 13.694001 22.837000 13.694001 11.398002 ] xS
-48 462 m
-(=##!"4.'\(\(#.2%#1'.'3'4%#\(-0'\(\(?A##H-5#I'?#'4#*%\(\(#/"4.'\(\(#.2%#*%3#4/.%#\(-0'\(\(?#*2/\(%#?-5#'&%#'3-5.#/.\)#'4#/.#/4#7&%..?#%'4?#.-#$%.)[ 15.990001 11.398002 11.398002 11.398002 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 20.500002 22.837000 9.143001 9.143001 20.500002 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 9.143001 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 20.500002 9.143001 11.398002 22.837000 11.398002 9.143001 22.837000 20.500002 22.837000 9.143001 9.143001 20.500002 11.398002 29.643002 22.837000 9.143001 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 9.143001 11.398002 11.398002 11.398002 22.837000 20.500002 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 ] xS
-48 514 m
-(57#'"1#&5""/"$)[ 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 ] xS
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-321.56699 514 m
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-/F1.1[ 41 0 0 -41 0 0]sf
-48 618 m
-(J6#0-5&4%\)#?-5#I/$2.#*'".#.-#B5I7#4.&'/$2.#.-#/"4.'\(\(/"$#.2%#8"489:;#*%3#4/.%\)#-&#.2%#1%\(,%#/".-#.2%#>%&\(#E>!A#K'@%#?-5&)[ 31.898003 11.398002 11.398002 20.500002 22.837000 22.837000 13.694001 20.500002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 34.194004 9.143001 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 9.143001 22.837000 34.194004 22.837000 11.398002 20.500002 11.398002 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 20.500002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 20.500002 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 13.694001 9.143001 11.398002 27.347002 27.347002 11.398002 11.398002 11.398002 25.051003 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 ] xS
-48 668 m
-(7/0@A)[ 22.837000 9.143001 20.500002 20.500002 11.398002 ] xS
-48 768 m
-(E\(.%&"'./,%\(?\)#?-5#"%%1#"-#\(-"$%%%\(#0-I7%\(\(%1#.-#54%#>%&\(#.-#*-&@#1/&%0.\(?#*/.2#)[ 27.347002 9.143001 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 20.500002 22.837000 9.143001 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 34.194004 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 27.347002 22.837000 13.694001 9.143001 11.398002 11.398002 22.837000 11.398002 29.643002 22.837000 13.694001 20.500002 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 ] xS
-1508 768 m
-(8"489:;#1'.'3'4%4A##!6#?-5#*'".#.-)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 ] xS
-48 818 m
-(*-&@#/"#L','#?-5#42-5\(1#1%6/"/.%\(?#2',%#'#\(--@#'.#.2%#&'7/1\(?#$&-*/"$#L','#E>!#F***A%"4%I3\(A-&$MB','MGA##K2/4#/4#"-.#')[ 29.643002 22.837000 13.694001 20.500002 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 9.143001 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 9.143001 20.500002 11.398002 22.837000 13.694001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 13.694001 29.643002 29.643002 29.643002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 20.500002 22.837000 11.398002 13.694001 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 ] xS
-48 868 m
-(453B%0.#0-,%&%1#/"#.2/4#1-05I%".\)#4-#/6#?-5#'&%#/".%&%4.%1#7\(%'4%#2',%#'#\(--@#'.#.2%#L','#47%0/6/0#1-05I%".'./-"A)[ 20.500002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 11.398002 20.500002 22.837000 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 ] xS
-48 968 m
-(N5&&%".\(?\)#'#&'.2%'&1#.2/"$#.-#1-#/4#.-#54%#.2%#8"489:;#4-6.*'&%#4?4.%I#.-#$%"%&'.%#6%'.5&%4#)[ 29.643002 22.837000 13.694001 13.694001 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 29.643002 22.837000 13.694001 22.837000 11.398002 20.500002 20.500002 20.500002 11.398002 22.837000 34.194004 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 ] xS
-/F1.1[ 41 0 8.7148399 -41 0 0]sf
-1799 968 m
-(1%#"-,-)[ 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 ] xS
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-1944.9 968 m
-(#F)[ 11.398002 13.694001 ] xS
-/F1.1[ 41 0 8.7148399 -41 0 0]sf
-1969.95 968 m
-(/A%A)[ 9.143001 11.398002 22.837000 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-2024.64 968 m
-(#&5"#.2%)[ 11.398002 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 1018 m
-($%"%#35/\(1/"$#7&-0%44G#6&-I#&'*#4%O5%"0%A##K2/4#/4#0'\(\(%1#.2%#P7/7%\(/"%PA##K-#/\(\(54.&'.%#.2/4\)#.2%&%#'&%#7&%4%".\(?#"5I%&-54)[ 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 20.500002 20.500002 13.694001 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 13.694001 22.837000 29.643002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 14.555001 22.837000 9.143001 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 14.555001 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 9.143001 9.143001 9.143001 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 13.694001 22.837000 22.837000 20.500002 ] xS
-48 1068 m
-(&%I-.%#8"489:;#*%3#4/.%4#35.#-"\(?#'#0-57\(%#-6#&%I-.%#7/7%\(/"%4A##Q%#%R7%0.#.2'.#1-05I%".'./-"#'"1#5"1%&4.'"1/"$#-6)[ 13.694001 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 20.500002 9.143001 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 38.704006 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 ] xS
-48 1118 m
-(.2%#7/7%\(/"%#*/\(\(#/I7&-,%#'4#I-&%#'"1#I-&%#7%-7\(%#'..%I7.#.-#&5"#/.#&%I-.%\(?A##K2%#1-05I%".'./-"#-6#.2%#7/7%\(/"%#.2'.)[ 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 9.143001 34.194004 22.837000 13.694001 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 34.194004 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 13.694001 22.837000 34.194004 22.837000 11.398002 22.837000 9.143001 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-48 1168 m
-(1-%4#%R/4.#/4#4.-&%1#/"#-5&#NSC#&%7-4/.-&?\)#*2/02#/4#%R.%&"'\(\(?#'00%44/3\(%A##>%&2'74#.2%#%'4/%4.#*'?#.-#-3.'/"#.2%4%)[ 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 20.500002 9.143001 20.500002 11.398002 11.398002 9.143001 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 29.643002 27.347002 27.347002 11.398002 13.694001 22.837000 22.837000 22.837000 20.500002 9.143001 11.398002 22.837000 13.694001 20.500002 11.398002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 9.143001 20.500002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 13.694001 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 ] xS
-48 1218 m
-(1-05I%".4#/"#.2&-5$2#-5&#*%3TNSC#F2..7<MM0,4*%3A4'"$%&A'0A5@M0$/T3/"M0,4*%3A0$/M%"4%I3\(T1-0MGA##H-5#'&%#5"\(/@%\(?#.-)[ 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 29.643002 22.837000 22.837000 13.694001 29.643002 27.347002 27.347002 11.398002 13.694001 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 11.398002 20.500002 20.500002 20.500002 29.643002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 9.143001 13.694001 22.837000 9.143001 22.837000 11.398002 20.500002 20.500002 20.500002 29.643002 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 13.694001 22.837000 22.837000 20.500002 11.398002 13.694001 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 20.500002 22.837000 9.143001 20.500002 11.398002 11.398002 22.837000 ] xS
-48 1268 m
-($%.#.2%#7/7%\(/"%#*-&@/"$#UVVW#7&-7%&\(?#*/.2-5.#'#3/.#-6#2%\(7\)#4-#/6#?-5#'&%#2%'1/"$#/"#.2/4#1/&%0./-"#\(%.#54#@"-*#'"1#4'?)[ 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 36.490002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 13.694001 9.143001 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 20.500002 22.837000 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 29.643002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 ] xS
-48 1318 m
-(2%\(\(-A)[ 22.837000 22.837000 9.143001 9.143001 22.837000 11.398002 ] xS
-48 1423 m
-(X-&#-.2%"489:;#1-05I%".'./-"\)#2',%#'#3&-*4%#.2&-5$2#.2%#8"489:;#*%3#4/.%#F)[ 25.051003 22.837000 13.694001 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 22.837000 13.694001 22.837000 29.643002 20.500002 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 20.500002 9.143001 11.398002 22.837000 11.398002 13.694001 ] xS
-0 0 1 sc
-1577.1899 1423 m
-(***A%"4%I3\(A-&$)[ 29.643002 29.643002 29.643002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 ] xS
-0 0 0 sc
-1903.03 1423 m
-(G#'"1#6-\(\(-*#.2%)[ 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 11.398002 11.398002 22.837000 22.837000 ] xS
-0 0 1 sc
-CM
-406.47998 468.76001 m
-484.72 468.76001 l
-484.72 468.28 l
-406.47998 468.28 l
-h
-f
-0 0 0 sc
-0.23999999 0 0 -0.23999999 28 811 cm
-48 1473 m
-(PY-05I%".'./-"P#\(/"@A##K2/4#42-5\(1#\(%'1#?-5#.-#'#"5I3%&#-6#54%65\(#P3/$P#1-05I%".4\)#/"0\(51/"$#.2%#-"%#?-5#'&%#7&%4%".\(?)[ 14.555001 29.643002 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 14.555001 11.398002 9.143001 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 11.398002 14.555001 22.837000 9.143001 22.837000 14.555001 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 11.398002 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 20.500002 ] xS
-48 1523 m
-(&%'1/"$#'"1#.2%#*%3T4/.%#/"4.'\(\('./-"#/"4.&50./-"4A)[ 13.694001 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 13.694001 20.500002 9.143001 11.398002 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 ] xS
-48 1623 m
-(X-&#I-&%#1%.'/\(%1#1-05I%".4#0\(/0@#-"#.2%#PQ/@/P#\(/"@#6&-I#.2%#PY-05I%".'./-"P#7'$%A###C-I%#-6#.2%#1-05I%".4#','/\('3\(%)[ 25.051003 22.837000 13.694001 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 20.500002 9.143001 9.143001 20.500002 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 14.555001 38.704006 9.143001 20.500002 9.143001 14.555001 11.398002 9.143001 9.143001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 14.555001 29.643002 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 14.555001 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 11.398002 27.347002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 9.143001 22.837000 ] xS
-48 1673 m
-(/"#.2%#Q/@/#'&%#57#.-#1'.%\)#4-I%#FI-4.G#'&%#"-.#T#0'5./-"A##J"0%#?-5#'&%#/"#.2%#Q/@/#7'$%4#?-5#0'"#54%#.2%#+4%'&02+#35..-")[ 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 38.704006 9.143001 20.500002 9.143001 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 13.694001 34.194004 22.837000 20.500002 11.398002 13.694001 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 13.694001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 31.898003 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 38.704006 9.143001 20.500002 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 7.831001 20.500002 22.837000 22.837000 13.694001 20.500002 22.837000 7.831001 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 1723 m
-(.-#6/"1#*2'.#?-5#'&%#\(--@/"$#6-&A##H-5#0'"#'\(4-#3&-*4%#.2%#'&02/,%4#-6#.2%#%"4%I3\(T1%,#I'/\(/"$#\(/4.#6-/40544/-"4#*2/02)[ 11.398002 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 29.643002 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 20.500002 22.837000 9.143001 20.500002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 13.694001 22.837000 22.837000 20.500002 11.398002 34.194004 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 9.143001 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 ] xS
-48 1773 m
-(I'?#2',%#'\(&%'1?#'"4*%&%1#4-I%#-6#?-5&#O5%4./-"4A##!6#'6.%-/"$#.2/4#?-5#4./\(\(#0'"+.#6/"1#*2'.#?-5#"%%1\)#7\(%'4%#6%%\(#6&%%)[ 34.194004 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 13.694001 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 29.643002 22.837000 13.694001 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 11.398002 9.143001 9.143001 9.143001 11.398002 20.500002 22.837000 22.837000 7.831001 11.398002 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 11.398002 11.398002 13.694001 22.837000 22.837000 ] xS
-48 1824 m
-(.-#7-4.#'#O5%4./-"#-"#.2%#%"4%I3\(T1%,Z%3/A'0A5@#I'/\(/"$#\(/4.A##Q%#&%47-"1#O5/0@\(?#'"1#'&%#"/0%#7%-7\(%#.2'.#1-"+.#3/.%\))[ 11.398002 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 13.694001 22.837000 22.837000 20.500002 41.656006 22.837000 22.837000 9.143001 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 34.194004 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 11.398002 11.398002 38.704006 22.837000 11.398002 13.694001 22.837000 20.500002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 9.143001 11.398002 22.837000 11.398002 ] xS
-48 1874 m
-(%,%"#/6#?-5#2',%"+.#3-.2%&%1#.-#&%'1#.2%#1-05I%".'./-"#F*2%&%#/.#%R/4.4GA)[ 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 13.694001 29.643002 22.837000 22.837000 13.694001 22.837000 11.398002 9.143001 11.398002 11.398002 22.837000 20.500002 9.143001 20.500002 11.398002 20.500002 13.694001 11.398002 ] xS
-48 1974 m
-(X/"'\(\(?\)#/6#?-5#6/"1#4-I%.2/"$#/"#.2/4#1-05I%".#.2'.#/4#"-.#0\(%'&#-&#/4#/"0-&&%0.\)#*%#*/\(\(#3%#I-4.#$&'.%65\(#/6#?-5#\(%.#54#@"-*)[ 25.051003 9.143001 22.837000 22.837000 9.143001 9.143001 20.500002 11.398002 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 9.143001 22.837000 22.837000 13.694001 11.398002 22.837000 13.694001 11.398002 9.143001 20.500002 11.398002 9.143001 22.837000 20.500002 22.837000 13.694001 13.694001 22.837000 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 11.398002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 29.643002 ] xS
-48 2024 m
-(-"#.2%#8"489:;#1%,%\(-7%&4#I'/\(/"$#\(/4.#F)[ 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 22.837000 13.694001 20.500002 11.398002 34.194004 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 13.694001 ] xS
-0 0 1 sc
-795.46899 2024 m
-(%"4%I3\(T1%,Z%3/A'0A5@)[ 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 13.694001 22.837000 22.837000 20.500002 41.656006 22.837000 22.837000 9.143001 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 ] xS
-0 0 0 sc
-1235 2024 m
-(GA##!6#?-5#'&%#42?\)#?-5#0'"#'\(4-#%I'/\(#I%#1/&%0.\(?)[ 13.694001 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 34.194004 22.837000 9.143001 9.143001 11.398002 34.194004 22.837000 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 ] xS
-0 0 1 sc
-CM
-218.8 324.52002 m
-324.39999 324.52002 l
-324.39999 324.04001 l
-218.8 324.04001 l
-h
-f
-0 0 0 sc
-0.23999999 0 0 -0.23999999 28 811 cm
-48 2074 m
-(F)s
-0 0 1 sc
-61.653301 2074 m
-(1.'Z4'"$%&A'0A5@)[ 22.837000 11.398002 22.837000 41.656006 20.500002 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 ] xS
-0 0 0 sc
-395.01901 2074 m
-(G\)#!#1-"+.#3/.%#%/.2%&A)[ 13.694001 11.398002 11.398002 11.398002 11.398002 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 9.143001 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 22.837000 22.837000 13.694001 11.398002 ] xS
-0 0 1 sc
-CM
-42.880001 312.52002 m
-122.8 312.52002 l
-122.8 312.04001 l
-42.880001 312.04001 l
-h
-f
-0 0 0 sc
-0.23999999 0 0 -0.23999999 28 811 cm
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2223 m
-(!"#$%"&'\(\("\)\)&%$&*+\)*,-.&/01&,234.5)[ 29.643002 22.837000 36.490002 25.051003 13.694001 22.837000 11.398002 29.643002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 25.051003 11.398002 27.347002 25.051003 22.837000 27.347002 34.194004 29.643002 25.051003 11.398002 22.837000 11.398002 22.837000 11.398002 34.194004 22.837000 27.347002 31.898003 25.051003 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2336 m
-(8"489:;#7&-,/1%4#'"#/".%&"%.#'00%44/3\(%#2-4.#F@'@'A4'"$%&A'0A5@G#*/.2#.2%#\('.%4.#1'.'3'4%4A#K2/4#I%'"4#?-5#0'"#1-#')[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 20.500002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 13.694001 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 34.194004 22.837000 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 ] xS
-48 2386 m
-(\(-.#-6#*-&@#6&-I#'"#/".%&"%.#0-""%0.%1#2-4.#4-\(%\(?#3?#54/"$#P0\(/%".P#4-6.*'&%A)[ 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 13.694001 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 9.143001 22.837000 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 14.555001 20.500002 9.143001 9.143001 22.837000 22.837000 11.398002 14.555001 11.398002 20.500002 22.837000 11.398002 11.398002 29.643002 22.837000 13.694001 22.837000 11.398002 ] xS
-48 2486 m
-(H-5#7&-3'3\(?#2',%#'#9?CD;#0\(/%".#/"4.'\(\(%1#/6#?-5#'&%#&5""/"$#'#4.'"1'&1#)[ 27.347002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 34.194004 20.500002 27.347002 31.898003 22.837000 11.398002 20.500002 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 22.837000 11.398002 ] xS
-1385 2486 m
-(\(/"5R#1/4.&/35./-"A##K&?<)[ 9.143001 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 20.500002 11.398002 13.694001 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 25.051003 13.694001 20.500002 11.398002 ] xS
-/F4.1[ 41 0 0 -41 0 0]sf
-48 2587 m
-(!"#$%&'\(&\)*+*"!+\(#&',&-\)-\).#\)*/01.\)2.\(-)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2688 m
-(!6#9?CD;#/4#"-.#/"4.'\(\(%1\)#$-#.-#)[ 11.398002 11.398002 11.398002 34.194004 20.500002 27.347002 31.898003 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 ] xS
-0 0 1 sc
-615.43402 2688 m
-(***AI?4O\(A0-I)[ 29.643002 29.643002 29.643002 11.398002 34.194004 20.500002 20.500002 22.837000 9.143001 11.398002 20.500002 22.837000 34.194004 ] xS
-0 0 0 sc
-911 2688 m
-(#.-#1-*"\(-'1#.2%#0\(/%".A)[ 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 29.643002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 ] xS
-0 0 1 sc
-CM
-175.59999 165.16003 m
-246.64 165.16003 l
-246.64 164.67999 l
-175.59999 164.67999 l
-h
-f
-0 0 0 sc
-0.23999999 0 0 -0.23999999 28 811 cm
-48 2788 m
-(!"4/1%#.2%#9?CD;#7&-I7.#?-5#$%"%&'\(\(?#*'".#.-#4.'&.#*/.2#.2%#1'.'3'4%#P2-I-[4'7/%"4[0-&%[U\\[]]P#F'.#./I%#-6#*&/./"$)[ 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 20.500002 27.347002 31.898003 22.837000 11.398002 22.837000 13.694001 22.837000 34.194004 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 22.837000 9.143001 9.143001 20.500002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 14.555001 22.837000 22.837000 34.194004 22.837000 22.837000 20.500002 22.837000 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 14.555001 11.398002 13.694001 22.837000 11.398002 11.398002 11.398002 9.143001 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 29.643002 13.694001 9.143001 11.398002 9.143001 22.837000 22.837000 ] xS
-48 2838 m
-(.2/4#*'4#.2%#I-4.#&%0%".#35/\(1G#'"1#.2%#1'.'3'4%#P%"4%I3\([I'&.[U\\[UPA##K2%&%#'&%#'\(4-#1'.'3'4%4#6-&#-.2%/%0/%4#T)[ 11.398002 22.837000 9.143001 20.500002 11.398002 29.643002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 9.143001 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 14.555001 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 34.194004 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 14.555001 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 22.837000 20.500002 11.398002 13.694001 ] xS
-48 2888 m
-(?-5#42-5\(1#3%#'3\(%#.-#54%#.2%4%#/"#.2%#4'I%#*'?#'4#1%40&/3%1#2%&%#6-I'"A)[ 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 29.643002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 ] xS
-48 2992 m
-(K&?#.2%#)[ 25.051003 13.694001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F4.1[ 41 0 0 -41 0 0]sf
-186.976 2992 m
-(3#,+4&5\)6\)7\)#0#3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-580.64001 2992 m
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-(&*&+,$&!"#\(!-&./,$#$&01''12\(!-&+,$&/1!3$!+\(1!&10&&.4$/\($.&+"-5&6!%$7./17$5)[ 11.398002 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 9.143001 22.837000 22.837000 11.398002 20.500002 20.500002 22.837000 22.837000 34.194004 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 20.500002 20.500002 22.837000 13.694001 22.837000 11.398002 ] xS
-48 263 m
-(%"+"8".$&+94$5&6!%$7./17$&"!%&&7$'$".$&!6#8$7:&&;"3$&"&'11\)&"716!%&+1&.$$&2,"+&916&/"!&6.$:)[ 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 20.500002 20.500002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 11.398002 11.398002 29.643002 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 ] xS
-48 368 m
-(<+"7+\(!-&2\(+,&+,$&=/17$=&%"+"8".$&>,1#1?."4\($!.?/17$?@A?BB&17&+,$.+&7$/$!+&64%"+$&10&+,\(.&%"+"8".$C5&+79&.1#$&10)[ 27.347002 11.398002 22.837000 13.694001 11.398002 9.143001 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 14.555001 20.500002 22.837000 13.694001 22.837000 14.555001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 20.500002 22.837000 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 13.694001 11.398002 11.398002 11.398002 13.694001 20.500002 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 ] xS
-48 418 m
-(+,$.$&!\(/$&D6$7\($.&+1&-$+&"&+".+$&10&2,"+&\(.&.+17$%&\(!&+,$&%"+"8".$.E)[ 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 ] xS
-48 518 m
-(F,11.$&+,$&/677$!+&,6#"!&/17$&%"+"8".$E)[ 29.643002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 619 m
-(!0123"#$#%&'\(\)*+&%4#5*%./%667!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 720 m
-(G$+7\($3$&+,$&0\(7.+&+21&HHIJKLMINKO$!I"!\)&$!+7\($.&.+17$%&\(!&+,$&%"+"8".$E)[ 29.643002 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 29.643002 29.643002 27.347002 20.500002 11.398002 27.347002 34.194004 27.347002 22.837000 11.398002 31.898003 22.837000 22.837000 27.347002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 820 m
-(!12829:34,#+*%\);3<=>?34,#+*38@?@:3A7!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 921 m
-(G$+7\($3$&+,$&0\(7.+&+21&/1!0\(7#$%&-$!$.&\(!&+,$&%"+"8".$E)[ 29.643002 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 13.694001 34.194004 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1021 m
-(!12829:3B3<=>?3C*+*38@?@:3A7!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1122 m
-(G$+7\($3$&+,$&HHIJKLMINKO$!I"!\)&$!+7\($.&071#&+,$&0\(7.+&PM8&10&,6#"!&/,71#1.1#$&@:&Q,$&"..$#8'9&\(!017#"+\(1!&\(.)[ 29.643002 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 29.643002 29.643002 27.347002 20.500002 11.398002 27.347002 34.194004 27.347002 22.837000 11.398002 31.898003 22.837000 22.837000 27.347002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 34.194004 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 9.143001 20.500002 ] xS
-48 1171 m
-(.+17$%&\(!&+,$&"..$#8'9&+"8'$5&,$!/$&2$&!$$%&+1&4$7017#&"&R1\(!&"/71..&+,$&)[ 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 34.194004 22.837000 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 13.694001 34.194004 11.398002 22.837000 11.398002 9.143001 22.837000 9.143001 22.837000 11.398002 22.837000 20.500002 13.694001 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1378.96 1171 m
-(4,#+*)[ 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1501.98 1171 m
-(5&)[ 11.398002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1524.76 1171 m
-(4#+-\)C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1673 1171 m
-(5&)[ 11.398002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1695.78 1171 m
-(4"5#$#&#$*)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1941.8199 1171 m
-(&"!%&)[ 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-2033.01 1171 m
-('&&*$D,E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1225 m
-(+"8'$.)[ 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 ] xS
-/F3.1[ 47 0 0 -47 0 0]sf
-157.407 1225 m
-(!)s
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1327 m
-(&=)[ 11.398002 14.555001 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-73.945297 1327 m
-(12829:3F@1:@G9:H)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-467 1327 m
-(4,#+*I+'$*J)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1374 m
-(3<=>?333'&&*$D,EK34#+-\)CK34,#+*K34"5#$#&#$*)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1420 m
-(3LM2=2334"5#$#&#$*I+'$*3N3O.O)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1466 m
-(3PGF33334"5#$#&#$*I4"5#$#&#$*%\);3N3'&&*$D,EI4"5#$#&#$*%\);)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1512 m
-(3PGF3333'&&*$D,EI4"5%*+;3Q3RSSSSSS)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1558 m
-(3PGF3333'&&*$D,EI4#+-\)C%\);3N34#+-\)CI4#+-\)C%\);)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1604 m
-(3PGF33334,#+*I4,#+*%\);3N34#+-\)CI4,#+*%\);7!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1705 m
-(L"/,&+"8'$&,".&"&6!\(D6$5&/1!.\(.+$!+'9*!"#$%&\(%$!+\(0\($7&2,\(/,&/"!&8$&6.$%&".&017$\(-!&\)$9.&\(!&1+,$7&+"8'$.:&&Q,$&6!\(D6$)[ 27.347002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 20.500002 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 9.143001 22.837000 13.694001 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 22.837000 ] xS
-48 1754 m
-(\(%$!+\(0\($7&/1'6#!&\(.&"'2"9.&!"#$%&&)[ 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 9.143001 22.837000 13.694001 11.398002 20.500002 22.837000 9.143001 22.837000 34.194004 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 9.143001 29.643002 22.837000 20.500002 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 ] xS
-/F4.1[ 41 0 8.7148399 -41 0 0]sf
-683.78003 1754 m
-(!"#$%&"'%)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-905.216 1754 m
-(%\);3)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1003 1754 m
-("!%&/1!+"\(!.&\(!+$-$7&3"'6$.:&&S17&$T"#4'$5&+,$&6!\(D6$&\(%$!+\(0\($7&10)[ 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 9.143001 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 13.694001 11.398002 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 9.143001 22.837000 13.694001 11.398002 22.837000 11.398002 ] xS
-48 1805 m
-(+,$&)[ 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-116.387 1805 m
-(4"5#$#&#$*)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-362.427 1805 m
-(&+"8'$&\(.&+,$&/1!+"\(!$%&\(!&+,$&)[ 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-884.396 1805 m
-(4"5#$#&#$*%\);)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1204.25 1805 m
-(&/1'6#!&"!%&+,$&712&2\(+,&)[ 11.398002 20.500002 22.837000 9.143001 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 29.643002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1669.14 1805 m
-(4"5#$#&#$*%\);3N3A6)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1857 m
-(/177$.41!%.&+1&+,$&/,71#1.1#$&!"#$%&UVU:)[ 20.500002 22.837000 13.694001 13.694001 22.837000 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 7.831001 27.347002 7.831001 11.398002 ] xS
-48 1957 m
-(G$+7\($3$&+,$&$T1!.)[ 29.643002 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 20.500002 ] xS
-389 1957 m
-(&2,\(/,&'\($&2\(+,\(!&+,$&0\(7.+&WXX5XXX&8".$*4"\(7.&10&/,71#1.1#$&@E)[ 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 9.143001 9.143001 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 9.143001 13.694001 20.500002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2048 m
-(!12829:3*T#+I*T#+%\);)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2094 m
-(3<=>?333*T#+K3'&&*$D,EK34"5#$#&#$*)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2140 m
-(3LM2=2334"5#$#&#$*I+'$*3N3O.O)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2186 m
-(3PGF33334"5#$#&#$*I4"5#$#&#$*%\);3N3'&&*$D,EI4"5#$#&#$*%\);)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2232 m
-(3PGF3333'&&*$D,EI4"5%*+;3Q3RSSSSS)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2278 m
-(3PGF3333'&&*$D,EI4#+-\)C%\);3N3*T#+I4#+-\)C%\);7!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2384 m
-(>Y1+$E&<\(.+\(!-&L!.LMIN&6.$7.&#"9&!1+\(/$&+,"+&+,$&)[ 13.694001 29.643002 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 27.347002 20.500002 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 11.398002 34.194004 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-1000 2384 m
-($T1!:\(%&/1'6#!&,".&8$$!&7$4'"/$%&89&$T1!:$T1!?\(%&2,\(/,&!12)[ 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 9.143001 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 29.643002 ] xS
-48 2434 m
-(/1!+"\(!.&"!&\(!+$-$7&3"'6$:&&Q1&-$+&+,$&LY<L&!6#8$7&916&2\(''&!$$%&+1&R1\(!&+1&$T1!?.+"8'$:&&I$&"2"7$&+,"+&+,$&!$2$.+)[ 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 29.643002 27.347002 27.347002 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 9.143001 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 11.398002 22.837000 29.643002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 22.837000 20.500002 11.398002 ] xS
-48 2484 m
-(7$'$".$&10&+,$&L!.LMIN&%"+"8".$&7$47$.$!+.&D6\(+$&"&8\(-&.,\(0+&\(!&+,$&./,$#"&/1#4"7$%&+1&47$3\(16.&7$'$".$.:C)[ 13.694001 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 22.837000 9.143001 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 20.500002 22.837000 9.143001 11.398002 11.398002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 20.500002 22.837000 22.837000 34.194004 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 13.694001 ] xS
-48 2584 m
-(Z+&+,\(.&41\(!+&916&/"!&.$$&+,"+&/1!.+76/+\(!-&D6$7\($.&"-"\(!.+&+,$&L!.LMIN&/17$&%"+"8".$&\(!&/,71#1.1#"'&/117%\(!"+$.&2\('')[ 27.347002 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 ] xS
-48 2634 m
-("'2"9.&#$"!&R1\(!\(!-&+1&+,$&"..$#8'9&+"8'$:&Q,\(.&\(.&"&8\(+&4"\(!06':&Q,"!\)06''9&2$&,"3$&%$3$'14$%&"&D6$79*14+\(#\(.$%)[ 22.837000 9.143001 29.643002 22.837000 20.500002 20.500002 11.398002 34.194004 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 34.194004 22.837000 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 25.051003 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 20.500002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 20.500002 13.694001 22.837000 22.837000 11.398002 9.143001 34.194004 9.143001 20.500002 22.837000 22.837000 ] xS
-48 2684 m
-(%"+"8".$&+,"+&\(.&%$7\(3$%&071#&+,\(.&%"+"8".$&>"&%"+"#"7+&\(!&+7$!%9&/1#46+$7&.4$"\)C5&/"''$%&)[ 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 13.694001 9.143001 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 34.194004 22.837000 13.694001 11.398002 11.398002 9.143001 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 22.837000 20.500002 13.694001 11.398002 11.398002 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 ] xS
-1679 2684 m
-(L!.LMIN&M"7+:&Q,\(.&\(.)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 34.194004 22.837000 13.694001 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 ] xS
-48 2734 m
-(/,"!-\(!-&7"4\(%'95&86+&\(.&2$''&217+,&4'"9\(!-&"716!%&2\(+,E)[ 20.500002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 29.643002 22.837000 9.143001 9.143001 11.398002 29.643002 22.837000 13.694001 11.398002 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2839 m
-(!0123*+&*$D,%$'5-%./%.7)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-613.89203 2839 m
-(=)s
-48 2940 m
-(Q1&&-$!$.&071#&"&4"7+\(/6'"7&7$-\(1!E)[ 25.051003 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 20.500002 22.837000 9.143001 22.837000 13.694001 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 3041 m
-(!12829:3C*+*%&-'D,*%\);3<=>?3"#$#%&'\(\)*+&%4#5*%C*+*3LM2=23)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1451 3041 m
-(4"5%+'$*3N3O.O3PGF)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
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-/Encoding 256 array 0 1 255{1 index exch/.notdef put}for
-
-dup 33 /g put
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-dup 34 /e put
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-dup 35 /n put
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-dup 36 /underscore put
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-dup 37 /c put
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-dup 38 /h put
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-dup 39 /r put
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-dup 40 /o put
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-dup 41 /m put
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-dup 42 /d put
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-dup 43 /space put
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-dup 44 /less put
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-dup 45 /two put
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-dup 46 /zero put
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-dup 47 /semicolon put
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-dup 48 /quotedbl put
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-dup 49 /S put
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-dup 50 /E put
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-dup 51 /L put
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-dup 52 /C put
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-dup 53 /T put
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-dup 54 /s put
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-dup 55 /t put
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-dup 56 /a put
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-dup 57 /b put
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-dup 58 /l put
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-dup 59 /i put
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-dup 60 /F put
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-dup 61 /R put
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-dup 62 /O put
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-dup 63 /M put
-
-dup 64 /p put
-
-dup 65 /W put
-
-dup 66 /H put
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-dup 67 /I put
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-dup 68 /K put
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-dup 69 /quotesingle put
-
-dup 70 /q put
-
-dup 71 /five put
-
-dup 72 /period put
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-dup 73 /percent put
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-dup 74 /A put
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-dup 75 /N put
-
-dup 76 /D put
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-dup 77 /equal put
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-dup 78 /one put
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-dup 79 /B put
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-dup 80 /hyphen put
-
-dup 81 /seven put
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-dup 82 /x put
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-dup 83 /v put
-
-dup 84 /colon put
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-dup 85 /at put
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-dup 86 /slash put
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-dup 87 /y put
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-dup 88 /w put
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-dup 89 /comma put
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-dup 90 /k put
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-dup 91 /u put
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-dup 92 /V put
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-dup 93 /U put
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-dup 94 /six put
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-readonly def
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-/FontMatrix [ 0.00048828125 0 0 0.00048828125 0 0 ] def
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-/FontBBox{-334 -839 1523 1793}def
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-/UniqueID 4288209 def
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-currentdict currentfile eexec
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-48 457 m
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-48 561 m
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-48 661 m
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-48 756 m
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-48 853 m
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-/F1.1[ 41 0 0 -41 0 0]sf
-48 957 m
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-294.04001 957 m
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-48 1061 m
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-48 1111 m
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-48 1161 m
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-48 1310 m
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-48 1424 m
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-48 1474 m
-(+%03#.'53#2ODP<#-*/35#%$#+%03#*/#4'-$#'#0'B#N#/%>#\)43"3Q5#@'-,3;D##E43B#>*--#\)'H3#B%,#\)4"%,A4#\)43#\("%+355#%$#+%//3+\)*/A#\)%)[ 20.500002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 34.194004 11.398002 9.143001 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 22.837000 9.143001 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 13.694001 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 22.837000 13.694001 22.837000 7.831001 20.500002 11.398002 20.500002 22.837000 9.143001 22.837000 22.837000 13.694001 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 11.398002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 20.500002 20.500002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 ] xS
-48 1524 m
-('/#9/59<=6#0'\)'.'53#'/0#'++355*/A#\)43#0'\)'#+%/\)'*/30#\)43"3*/D##E43#*/$%"&'\)*%/#+%/\)'*/30#43"3#*5#.B#/%#&3'/5)[ 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 22.837000 20.500002 ] xS
-48 1574 m
-(3?4',5\)*@3G#.,\)#54%,-0#A*@3#B%,#3/%,A4#H/%>-30A3#\)%#.3#'.-3#\)%#+%&\(3\)3/\)-B#,53#\)43#+%"3#9/59<=6#+%03D##K$\)3")[ 22.837000 20.500002 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 27.347002 11.398002 11.398002 22.837000 13.694001 ] xS
-48 1624 m
-(-3'"/*/A#4%>#\)%#"3\)"*3@3#A3/35G#3?%/5G#53C,3/+3#'/0#\("%+355#\)4353#\)%#B%,"#%>/#3/05G#>3#4%\(3#B%,#>*--#$33-#+%/$*03/\))[ 9.143001 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 22.837000 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 ] xS
-48 1674 m
-(3/%,A4#\)%#03-@3#033\(3"#*/\)%#%\)43"#-355N\)"%003/#\('"\)5#%$#\)43#+%03#\)4'\)#&'B#*/\)3"35\)#B%,D)[ 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 20.500002 20.500002 13.694001 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 34.194004 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 ] xS
-48 1774 m
-(=3$%"3#B%,#5\)'"\)G#B%,#>*--#/330#\)%#4'@3#\)43#"3-3@'/\)#9/59<=6#'/0#)[ 27.347002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 9.143001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-1253 1774 m
-(=*%M3"-#&%0,-35#*/5\)'--30D##E4353#'"3#1)[ 27.347002 9.143001 22.837000 27.347002 22.837000 13.694001 9.143001 11.398002 34.194004 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1869 m
-(9;\(@"':P.HQ+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-343.24799 1869 m
-(##2>3#H/%>#\)4*5#*5#/%\)#\)43#-'\)35\)#@3"5*%/#N#$%"#/%>G#9/59<=6#*5#\)*30#\)%#*\)G#5%#\(-3'53#,53#*\)D;)[ 11.398002 11.398002 13.694001 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 13.694001 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 9.143001 11.398002 11.398002 11.398002 20.500002 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 9.143001 11.398002 11.398002 13.694001 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1916 m
-("#6"\)9:)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1962 m
-("#6"\)9:P"R7"'#8:)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2059 m
-(7/5\)",+\)*%/5#%/#4%>#\)%#*/5\)'--#\)4353#\(3"-#&%0,-35#'"3#+%/\)'*/30#%/#\)43#9/59<=6#>3.5*\)3#)[ 11.398002 22.837000 20.500002 11.398002 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 13.694001 9.143001 11.398002 34.194004 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 29.643002 22.837000 22.837000 20.500002 9.143001 11.398002 22.837000 11.398002 ] xS
-0 0 1 sc
-1661 2059 m
-(>>>D3/53&.-D%"A)[ 29.643002 29.643002 29.643002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 ] xS
-0 0 0 sc
-1986.84 2059 m
-(D#='5*+'--BG)[ 11.398002 11.398002 27.347002 22.837000 20.500002 9.143001 20.500002 22.837000 9.143001 9.143001 20.500002 11.398002 ] xS
-0 0 1 sc
-CM
-426.63998 316.12003 m
-504.87997 316.12003 l
-504.87997 315.64001 l
-426.63998 315.64001 l
-h
-f
-0 0 0 sc
-0.23999999 0 0 -0.23999999 28 811 cm
-48 2109 m
-(B%,#/330#\)%#0%#\)43#$%--%>*/A#5\)3\(5#2*/#.%\)4#+'535#.3-%>#>3#'"3#,5*/A#+@5#\)%#A3\)#\)43#+%03G#>4*+4#*5#&,+4#.3\)\)3"#\)4'/#$\)\(#'5)[ 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 22.837000 20.500002 11.398002 13.694001 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 29.643002 11.398002 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 20.500002 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 34.194004 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 ] xS
-48 2159 m
-(>3#'"3#A3\)\)*/A#\)43#-'\)35\)#.,A#$*?35D#J%\)*+3#\)43#N#"#$-'A#\)%#\)43#+@5#+%&&'/05D#E4353#*/0*+'\)3#\)43#."'/+4#%$#3'+4#"3\(%5*\)%"B)[ 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 11.398002 13.694001 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 20.500002 20.500002 11.398002 20.500002 22.837000 34.194004 34.194004 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 20.500002 9.143001 11.398002 22.837000 13.694001 20.500002 ] xS
-48 2209 m
-(\)%#A3\)#%,\)D##2="'/+435#'"3#5\)'.-3#@3"5*%/5#%$#\)43#+%03;D)[ 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 11.398002 13.694001 27.347002 13.694001 22.837000 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 13.694001 11.398002 ] xS
-48 2309 m
-(#####=*%M3"-#2533#+@5D.*%\(3"-D%"A#$%"#&%"3#03\)'*-5;)[ 11.398002 11.398002 11.398002 11.398002 11.398002 27.347002 9.143001 22.837000 27.347002 22.837000 13.694001 9.143001 11.398002 13.694001 20.500002 22.837000 22.837000 11.398002 20.500002 20.500002 20.500002 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 13.694001 9.143001 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 20.500002 13.694001 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2374 m
-(%S6+P*+T@6"'S"'T%S6U%S6H9;\(@"':H\('!TV&\(\)"V'"@\(6;7\('WV9;\(@"':+:\(!;#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 8.7148399 -41 0 0]sf
-48 2420 m
-(X&"#+@'\(\)@7"*Y+7&"+@866X\('*+;6+E%S6E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2488 m
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-48 312 m
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-48 407 m
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-48 453 m
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-48 499 m
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-409 499 m
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-48 602 m
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-105 602 m
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-48 759 m
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-48 984 m
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-1096.16 984 m
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-48 1034 m
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-48 1084 m
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-385.14899 1084 m
-(#*%#*+.#.>.0:23.3#2\(:4&'.'#4,*.0#2\(#*+23#*&*%02,4C##N+.\(#$%&#:+.:OP%&*#,#:%=$#%"#5\(35678#"0%<#*+.#:-3/)[ 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 9.143001 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 13.694001 9.143001 22.837000 9.143001 11.398002 11.398002 11.398002 38.704006 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 20.500002 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 20.500002 20.500002 11.398002 ] xS
-48 1134 m
-(9%*+#*+.#:%<=,\(2%\(#3:02=*#,\('#*+.#=.\('2\(A#1%0O.'#3%4&*2%\(3#1244#9.#2\(#*+.#.\(3.<94Q'%:3Q*&*%02,4#'20.:*%0$C)[ 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 13.694001 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 13.694001 9.143001 22.837000 9.143001 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 22.837000 13.694001 20.500002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1283 m
-(56%,\(-".\)\(,6.\(*"+.\(7&\)789:\(-%,%;%\).\(*"&,%'&<)[ 38.704006 25.051003 22.837000 13.694001 11.398002 25.051003 25.051003 22.837000 22.837000 11.398002 13.694001 25.051003 22.837000 11.398002 22.837000 25.051003 15.990001 22.837000 11.398002 27.347002 25.051003 22.837000 27.347002 34.194004 29.643002 25.051003 11.398002 25.051003 22.837000 13.694001 22.837000 25.051003 22.837000 22.837000 22.837000 11.398002 22.837000 25.051003 25.051003 13.694001 22.837000 11.398002 25.051003 25.051003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1397 m
-(RB4%\(.3#;12*+#.<94#,::.332%\(3?#P#9%*+#"2\(23+.'#,\('#&\("2\(23+.'C)[ 15.990001 29.643002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 13.694001 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 34.194004 22.837000 9.143001 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 20.500002 13.694001 11.398002 13.694001 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 9.143001 22.837000 9.143001 20.500002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 9.143001 20.500002 22.837000 22.837000 22.837000 11.398002 ] xS
-48 1497 m
-(R5,:+#:%\(*2A&%&3#=2.:.#%"#3.J&.\(:.#23#:,44.'#,#:%\(*2AC##M+.3.#,0.#*+.#9,32:#4.\(A*+3#%"#ILK#*+,*#1.#,\(,4$3.#,\(')[ 15.990001 27.347002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 29.643002 29.643002 27.347002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 ] xS
-48 1547 m
-(,\(\(%*,*.C)[ 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 ] xS
-48 1647 m
-(R5,:+#:4%\(.#1244#:%\(*,2\(#%\(.#%0#<%0.#:%\(*2A3C)[ 15.990001 27.347002 22.837000 20.500002 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 ] xS
-48 1747 m
-(RS2\(23+.'#:4%\(.3##T#%\(.#:%\(*2AC)[ 15.990001 25.051003 9.143001 22.837000 9.143001 20.500002 22.837000 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 23.944002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 ] xS
-48 1847 m
-(RD\("2\(23+.'#:4%\(.3#T#,\($#\(&<9.0#%"#:%\(*2A3C)[ 15.990001 29.643002 22.837000 11.398002 9.143001 22.837000 9.143001 20.500002 22.837000 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 23.944002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 ] xS
-48 1947 m
-(R5,:+#:%\(*2A#;1+.*+.0#"2\(23+.'#%0#&\("2\(23+.'?#+,3#:.0*,2\(#".,*&0.3#,33%:2,*.'#12*+#2*#*+,*#0.=0.3.\(*#*+.#0.3&4*#,\($#%\(.#%")[ 15.990001 27.347002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 13.694001 29.643002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 9.143001 22.837000 9.143001 20.500002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 9.143001 20.500002 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 13.694001 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 9.143001 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 20.500002 22.837000 9.143001 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 ] xS
-48 1997 m
-(*+.#-,02%&3#,\(,4$323#=0%A0,<3#*+,*#+,-.#9..\(#0&\(#%\(#2*#;.CAC#U.=.,*6,3O.0/#78K@M/#A.\(3:,\(?C##M+.3.#,0.#*+.#9,32:)[ 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 13.694001 22.837000 34.194004 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 20.500002 22.837000 13.694001 11.398002 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 11.398002 11.398002 22.837000 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 13.694001 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 20.500002 ] xS
-48 2047 m
-(:%<=&*.3#&3.'#*%#9&24'#*+.#A.\(.3C)[ 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 ] xS
-48 2147 m
-(RM+.#0,1#,\(,4$323#0.3&4*3#,0.#&3.'#*%#9&24'#A.\(.3#1+2:+#,0.#,43%#3*%0.'#2\(#*+.#',*,9,3.C##5,:+#A.\(.#:%\(*,2\(3#%\(.#%0)[ 15.990001 25.051003 22.837000 22.837000 11.398002 13.694001 22.837000 29.643002 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 13.694001 22.837000 20.500002 22.837000 9.143001 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 ] xS
-48 2197 m
-(<%0.#*0,\(3:02=*3#,\('#.,:+#*0,\(3:02=*#1244#:%\(*,2\(#,#*0,\(34,*2%\(C)[ 34.194004 22.837000 13.694001 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 ] xS
-48 2297 m
-(R5,:+#A.\(.#+,3#-,02%&3#2\("%0<,*2%\(#,**,:+.'#*%#2*#'.3:0292\(A#1+.*+.0#2*#23#,#O\(%1\(#A.\(.#%0#:%00.3=%\('3#*%#,#@1233H0%*#)[ 15.990001 27.347002 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 13.694001 9.143001 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 9.143001 11.398002 11.398002 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 27.347002 29.643002 9.143001 20.500002 20.500002 27.347002 13.694001 22.837000 11.398002 11.398002 ] xS
-2207 2297 m
-(%0)[ 22.837000 13.694001 ] xS
-48 2347 m
-(*05678#=0%*.2\(C##@%<.#A.\(.3#,0.#\(%-.4#A.\(.3#1+2:+#+,-.#9..\(#9&24*#9$#2\(".0.\(:.#"0%<#32<24,02*2.3#*%#%*+.0#3.J&.\(:.3C)[ 11.398002 13.694001 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 20.500002 9.143001 34.194004 9.143001 9.143001 22.837000 13.694001 9.143001 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 ] xS
-48 2397 m
-(M+.3.#\(%-.4#A.\(.3#1%\(\)*#+,-.#,#:%00.3=%\('2\(A#@1233H0%*#%0#)[ 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 29.643002 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 20.500002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 27.347002 29.643002 9.143001 20.500002 20.500002 27.347002 13.694001 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 ] xS
-1158 2397 m
-(*05678#=0%*.2\(C)[ 11.398002 13.694001 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 ] xS
-48 2497 m
-(R5,:+#*0,\(34,*2%\(#+,3#+,'#,#-,02.*$#%"#=0%*.2\(#,\(,4$3.3#:%\('&:*.'#%\(#2*#,\('#$%&#:,\(#,::.33#2\("%0<,*2%\(#,9%&*#*+.#0.3&4*3)[ 15.990001 27.347002 22.837000 20.500002 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 20.500002 22.837000 9.143001 11.398002 20.500002 ] xS
-48 2547 m
-(%"##*+.3.C##M+23#2\(:4&'.3#2\("%0<,*2%\(#'.02-.'#"0%<#=",</#=0%32*.#,\('#=02\(*3C)[ 22.837000 11.398002 11.398002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 9.143001 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 22.837000 34.194004 11.398002 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 ] xS
-48 2647 m
-(RM+.0.#,0.#%*+.0#".,*&0.3#,::.33294.#*+0%&A+#\(%\(P:%0.#5\(35678#.>*.0\(,4#',*,9,3.3C###@&:+#',*,9,3.3#<,$#:%\(*,2\()[ 15.990001 25.051003 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 20.500002 22.837000 13.694001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 11.398002 11.398002 27.347002 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 34.194004 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 ] xS
-48 2697 m
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-48 2747 m
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-/F3.1[ 41 0 0 -41 0 0]sf
-48 2896 m
-(=.,0$)[ 27.347002 22.837000 13.694001 25.051003 25.051003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 3010 m
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-48 3060 m
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-dup 61 /B put
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-dup 62 /dollar put
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-dup 63 /d put
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-dup 64 /u put
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-dup 65 /colon put
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-dup 66 /p put
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-dup 67 /hyphen put
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-dup 68 /zero put
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-dup 69 /period put
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-dup 70 /seven put
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-dup 71 /x put
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-dup 72 /w put
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-dup 73 /D put
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-dup 74 /Q put
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-dup 75 /A put
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-dup 76 /semicolon put
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-dup 77 /y put
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-dup 78 /equal put
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-dup 79 /quotesingle put
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-dup 80 /k put
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-dup 81 /g put
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-dup 82 /underscore put
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-dup 83 /one put
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-dup 84 /six put
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-dup 85 /three put
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-dup 86 /parenleft put
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-dup 87 /greater put
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-dup 88 /comma put
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-dup 89 /parenright put
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-readonly def
-
-/FontMatrix [ 0.00048828125 0 0 0.00048828125 0 0 ] def
-
-/FontBBox{-334 -839 1523 1793}def
-
-/UniqueID 4288209 def
-
-currentdict currentfile eexec
-
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-/F1.1[ 41 0 0 -41 0 0]sf
-48 262 m
-(!"#$#%&'\(\)%*#%+$&,\(',-.#$+\)$/\)$+"'0$'0$123454678$$69$:\);$,\(#$;0'%<$=0"$$\)\($+=0"$:\);$%##/$+\)$+:>#$'%$+"#$9\)..\)?'%<)[ 25.051003 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 13.694001 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 27.347002 27.347002 29.643002 22.837000 22.837000 22.837000 11.398002 27.347002 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 20.500002 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 20.500002 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 ] xS
-48 312 m
-(@=",%<'%<$A%90A=\(\)=A*'="#.#A-\(,%="$+\)$:\);\($/'\(#=+\)\(:$=\)%+,'%'%<$+"#$>#\(.$*\)/;.#0$:\);$/\)?%.\),/#/B8$$69$:\);$,\(#)[ 13.694001 20.500002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 11.398002 20.500002 13.694001 22.837000 20.500002 11.398002 34.194004 9.143001 20.500002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 22.837000 13.694001 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 9.143001 11.398002 34.194004 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 22.837000 9.143001 22.837000 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 ] xS
-48 362 m
-(=\)%=\)*'+,%+.:$;0'%<$,$&#\(0'\)%$\)9$7'\)1#\(.$%#?#\($+",%$&#\(0'\)%$C8D$*,E#$0;\(#$+",+$+"#$\)./#\($&#\(0'\)%$,>>#,\(0$'%$:\);\($1#\(.)[ 20.500002 22.837000 22.837000 20.500002 22.837000 34.194004 9.143001 11.398002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 27.347002 9.143001 22.837000 27.347002 22.837000 13.694001 9.143001 11.398002 22.837000 22.837000 29.643002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 34.194004 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 13.694001 20.500002 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 27.347002 22.837000 13.694001 9.143001 ] xS
-48 412 m
-(.'-\(,\(:$>,+"$-#9\)\(#$+"#$%#?#\($&#\(0'\)%8)[ 9.143001 9.143001 22.837000 13.694001 22.837000 13.694001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 22.837000 13.694001 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 507 m
-(!"#"$%&'\(\)*+,'&-$.!-/01/-23/4"5"-607$/4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 603 m
-(!"#"$%&8'9:;:<=&>'\(\)*+,'-"$!"265-21?@5"!A>'\(\)*+,'-631B"05CDEFA>'\(\)*+,'-"$!"265C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 649 m
-("G#"0$75-21?@5"!A>'\(\)*+,'-"$!"265C53#"-21?@5"!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 753 m
-(6+$",0$-#=\)*#$,>>,\(#%+$\(#=#%+.:$+",+$+"#$2%02F74$=\)/#G-,0#$H;0+$?\)%I+$\(;%$\)%$\)./#\($&#\(0'\)%0$\)9$)[ 11.398002 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 22.837000 7.831001 11.398002 11.398002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 ] xS
-1810 753 m
-(1#\(.8$$J#$;0#$&#\(0'\)%)[ 27.347002 22.837000 13.694001 9.143001 11.398002 11.398002 11.398002 38.704006 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 ] xS
-48 803 m
-(58K$9\)\($/#&#.\)>*#%+$,%/$+#0+'%<8$$J#$\(#=\)**#%/$+",+$:\);$0"\);./$/\)$0\)$+\)\)8$$69$:\);$,\(#$;%0;\(#$\)9$?",+$&#\(0'\)%$'0$\(;%%'%<)[ 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 38.704006 22.837000 11.398002 13.694001 22.837000 20.500002 22.837000 34.194004 34.194004 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 13.694001 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 ] xS
-48 853 m
-(\)%$:\);\($*,="'%#8$!:>#L)[ 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 34.194004 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 25.051003 20.500002 22.837000 22.837000 11.398002 ] xS
-48 953 m
-(>#\(.$G&)[ 22.837000 22.837000 13.694001 9.143001 11.398002 13.694001 20.500002 ] xS
-48 1056 m
-(1\(#0;*'%<$+",+$+"'0$'0$,..$'%$\)\(/#\(M$:\);$,\(#$%\)?$\(#,/:$+\)$?\('+#$:\);\($9'\(0+$0=\('>+8)[ 27.347002 13.694001 22.837000 20.500002 22.837000 34.194004 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 9.143001 9.143001 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 13.694001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 13.694001 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 29.643002 13.694001 9.143001 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1205 m
-(!"##$%&'"#)[ 29.643002 25.051003 25.051003 25.051003 22.837000 22.837000 13.694001 11.398002 25.051003 25.051003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1319 m
-(N..$/,+,$;0#/$,%/$=\(#,+#/$-:$2%02F74$'0$0+\)\(#/$'%$,$F:OP4$\(#.,+'\)%,.$/,+,-,0#8$$69$:\);$?,%+$+\)$,==#00$+"'0$/,+,-,0#$+"#)[ 27.347002 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 9.143001 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 34.194004 20.500002 27.347002 31.898003 22.837000 11.398002 13.694001 22.837000 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 9.143001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 1369 m
-(9'\(0+$+"'%<$:\);$",&#$+\)$/\)$'0$+\)$=\)%%#=+$+\)$'+8$$!"'0$'0$/\)%#$-#"'%/$+"#$0=#%#0$-:$2%02F74)[ 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 11.398002 22.837000 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 11.398002 9.143001 11.398002 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 20.500002 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 ] xS
-1661 1369 m
-($;0'%<$+"#$9,*\);0$1#\(.$*\)/;.#)[ 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 34.194004 22.837000 22.837000 20.500002 11.398002 27.347002 22.837000 13.694001 9.143001 11.398002 34.194004 22.837000 22.837000 22.837000 9.143001 22.837000 ] xS
-48 1419 m
-(=,..#/$Q768$$R\);$?'..$%##/$+\)$E%\)?$+"\(##$+"'%<0$-#9\)\(#$:\);$0+,\(+$L)[ 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 29.643002 27.347002 11.398002 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 11.398002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1514 m
-(&&&41!#&)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-259 1514 m
-(#4"&41!#$72"&H4"0""&'$!',=:&?7#767!"&53%"!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1560 m
-(&&&?6$72")[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-409 1560 m
-(#4"&$72"&1."&'$!',=:&?7#767!")[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1606 m
-(&&&@!"0)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-259 1606 m
-(#4"&@!"0$72"&7//"!!"&?7#767!")[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1707 m
-(S'\(0+M$?#$%##/$+\)$/#=.,\(#$+\)$1#\(.$+"#$*\)/;.#0$?#$?,%+$+\)$;0#$0\)$'+$=,%$<\)$,%/$="#=E$+"#$0:%+,T$\)9$+"#*8$$!"'0$'0$/\)%#$-:)[ 25.051003 9.143001 13.694001 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 11.398002 27.347002 22.837000 13.694001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 29.643002 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 ] xS
-48 1757 m
-(,$;0#$0+,+#*#%+8$$!"'0$.'%#$",0$+\)$-#$'%0#\(+#/$'%+\)$,..$:\);\($2%02F74$0=\('>+0L)[ 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 20.500002 11.398002 22.837000 11.398002 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1852 m
-(@!"&=31AA'$!',=:AAI=\)J:AAI=K?7B#10L)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1953 m
-(!"#$?#$0#+$+"#$,..$'*>\)\(+,%+$&,\(',-.#0$+#..'%<$1#\(.$?"#\(#$,%/$?",+$:\);\($/,+,-,0#$'0L)[ 25.051003 22.837000 22.837000 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 9.143001 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 13.694001 9.143001 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 27.347002 22.837000 13.694001 9.143001 11.398002 29.643002 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2044 m
-(2M&>41!#&&&N&OP7P7E!7$Q"0E7/E@POL)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2090 m
-(2M&>@!"0&&&N&O7$1$M21@!OL)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2136 m
-(2M&>?6$72"&N&O4121R!7B3"$!R/10"RSTRUUOL)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2237 m
-(U\)?$?#$=,%$*,E#$,$/,+,-,0#$=\)%%#=+'\)%L)[ 29.643002 22.837000 29.643002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2332 m
-(2M&>?6&N&$"H&=31AA'$!',=:AAI=\)J:AAI=K?7B#10VC41!#&&&NW&>41!#X)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2378 m
-(&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&C@!"0&&&NW&>@!"0X)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2424 m
-(&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&&C?6$72"&NW&>?6$72"YL)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2524 m
-(J#I&#$*,/#$,$=\)%%#=+'\)%$+\)$,%$2%02F74$/,+,-,0#$,%/$>,00#/$>,\(,*#+#\(0$'%$;0'%<$+"#$)[ 38.704006 22.837000 7.831001 20.500002 22.837000 11.398002 34.194004 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 34.194004 22.837000 11.398002 22.837000 13.694001 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1669.48 2524 m
-(C7##036@#"&NW)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2575 m
-(O!12"%75@"O)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-318.64401 2575 m
-($0:%+,T$>\(#0#%+$'%$*,%:$\)9$+"#$2%02F74$\)-H#=+$=\)%0+\(;=+\)\(08$$S\)\(*,++#/$=\)\(\(#=+.:M$+"'0$0:%+,T$.#+0$:\);$0##)[ 11.398002 20.500002 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 11.398002 34.194004 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 20.500002 11.398002 22.837000 13.694001 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 13.694001 34.194004 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 ] xS
-48 2627 m
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-(;\(%#1&/1234#<=>#,--"9/#$,&'0*-,!'"&#"\)#!\(%#+,!,5,/%#+,!,#!\(."*?\(#8,.'"*/#5*/'&%//#"56%7!/:##@".#%A,$0-%B#/"$%#"\))[ 25.051003 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 20.500002 11.398002 34.194004 22.837000 22.837000 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 13.694001 11.398002 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 ] xS
-49 362 m
-(!\(%#$".%#\(%,8'-C#*/%+#"56%7!/#,.%#!\(%#D%&%B#E-"&%#,&+#1A"&#"56%7!/:##2".%#+%!,'-/#"\)#\("9#!"#%\)\)%7!'8%-C#*/%#!\(%/%)[ 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 9.143001 9.143001 20.500002 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 31.898003 22.837000 22.837000 22.837000 11.398002 11.398002 29.643002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 27.347002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 9.143001 20.500002 22.837000 9.143001 20.500002 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 ] xS
-49 412 m
-("56%7!/#9'--#5%#7"8%.%+#-,!%.:##;\(%/%#"56%7!/#,.%#.%!.'%8%+#,&+#/!".%+#'&#!\(%#+,!,5,/%#!\(."*?\(#!\(%#*/%#"\)#"56%7!#F,+,0!"./F:)[ 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 7.831001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 20.500002 7.831001 11.398002 ] xS
-49 462 m
-(;\(%#"56%7!#,+,0!"./#\(,8%#'&!%.&,-#G&"9-%+?%#"\)#!\(%#*&+%.-C'&?#+,!,5,/%#/7\(%$,#,&+#*/%#!\('/#G&"9-%+?%#!"#\)%!7\(B#/!".%)[ 25.051003 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 29.643002 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 9.143001 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 20.500002 22.837000 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 29.643002 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 ] xS
-49 512 m
-(,&+#.%$"8%#"56%7!/#H,&+#+,!,I#\)."$#!\(%#+,!,5,/%:##;\('/#9,C#C"*#7,	.'!%#7"+%#,&+#*/%#!\(%#1&/1234#<=>#9'!\("*!)[ 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 13.694001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 29.643002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 13.694001 9.143001 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 29.643002 9.143001 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-49 562 m
-(\(,8'&?#!"#G&"9#,&C!\('&?#,5"*!#!\(%#*&+%.-C'&?#+,!,5,/%/#C"*#,.%#*/'&?:##;\(%#+,!,5,/%#,+,0!".#H!\(,!#9%#"5!,'&%+#5C)[ 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 9.143001 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 13.694001 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 ] xS
-49 612 m
-(7"&&%7!'&?#!"#"*.#+,!,5,/%B#'&#!\(%#0.%8'"*/#/%7!'"&I#'/#,#/0%7',-#,+,0!".#9\('7\(#\(,/#!\(%#.%/0"&/'5'-'!C#"\)#$,'&!,'&'&?#!\(%)[ 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 13.694001 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 22.837000 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 20.500002 22.837000 22.837000 22.837000 20.500002 9.143001 22.837000 9.143001 9.143001 9.143001 11.398002 20.500002 11.398002 22.837000 11.398002 11.398002 34.194004 22.837000 9.143001 22.837000 11.398002 22.837000 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-49 662 m
-(+,!,5,/%#7"&&%7!'"&#,&+#7.%,!'&?#"!\(%.#"56%7!#,+,0!"./:)[ 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 13.694001 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 20.500002 11.398002 ] xS
-49 770 m
-(</#9'--#5%#/\("9&#-,!%.#'&#!\('/#+"7*$%&!B#!"#"5!,'&#"56%7!#,+,0!"./#\)."$#!\(%#$,'&#+,!,5,/%#,+,0!".#!\(%.%#'/#,#/*'!%#"\))[ 27.347002 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 20.500002 22.837000 9.143001 11.398002 22.837000 11.398002 22.837000 11.398002 ] xS
-49 819 m
-($%!\("+/#!\(,!#\(,8%#!\(%##&,$'&?#7"&8%&!'"&#J)[ 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 34.194004 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 14.555001 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-847.61902 819 m
-(!"#$)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-/F3.1[ 41 0 8.7148399 -41 0 0]sf
-946.03497 819 m
-(!"#$%&'\(\)*&+,-)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1290.49 819 m
-(:##;"#"5!,'&#,#K-'7%<+,0!".#".#,#E-"&%<+,0!".)[ 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 27.347002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 13.694001 11.398002 22.837000 11.398002 29.643002 9.143001 22.837000 22.837000 22.837000 27.347002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 ] xS
-49 871 m
-(H9\('7\(B#"++-C#%&"*?\(B#.%!.'%8%#K-'7%#,&+#E-"&%#"56%7!/I#+"#!\(%#\)"--"9'&?L)[ 13.694001 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 13.694001 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-49 966 m
-(%&'\(!"\)"$*+*,#-.'/'\(+012!"#$3"\)"4+*,#-.567)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-49 1012 m
-(%&'\(89-\)"$*+*,#-.'/'\(+012!"#$:9-\)"4+*,#-.567)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1113 m
-(M"&F!#9"..C#'\)#C"*#+"&F!#'$$%+',!%-C#/%%#\("9#*/%\)*-#!\('/#7"*-+#5%:##N*/!#.%$%$5%.#!\(,!#C"*#+"&F!#&%%+#!"#G&"9#,&C!\('&?)[ 29.643002 22.837000 22.837000 7.831001 11.398002 11.398002 29.643002 22.837000 13.694001 13.694001 20.500002 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 9.143001 34.194004 34.194004 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 20.500002 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 22.837000 22.837000 ] xS
-48 1163 m
-(,5"*!#\("9#!\(%#+,!,5,/%#'/#/!.*7!*.%+B#5*!#C"*#7,&#.%!.'%8%#!\(%#&%7%//,.C#+,!,#H&%,!-C#0,7G,?%+#'&#"56%7!/I#5C#,/G'&?#\)".)[ 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 20.500002 11.398002 13.694001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 20.500002 20.500002 22.837000 13.694001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 13.694001 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 ] xS
-48 1213 m
-('!#\)."$#!\(%#7"..%7!#,+,0!".:##;\(."*?\("*!#!\(%#.%/!#"\)#!\('/#+"7*$%&!#9%#,.%#?"'&?#!"#9".G#!\(."*?\(#!\(%#9,C/#!\(%#1&/1234)[ 9.143001 11.398002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 11.398002 25.051003 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 29.643002 22.837000 13.694001 20.500002 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 ] xS
-48 1262 m
-(5*/'&%//#"56%7!/#7,%#*/%+#!"#+%.'8%#!\(%#'&\)".$,!'"&#C"*#9,&!:)[ 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 ] xS
-/F4.1[ 41 0 0 -41 0 0]sf
-48 1424 m
-(!"#$%&'"#&%\(\)"&*+#+)[ 25.051003 22.837000 13.694001 9.758001 22.837000 11.398002 25.051003 22.837000 13.694001 11.398002 22.837000 25.051003 36.490002 22.837000 11.398002 25.051003 22.837000 13.694001 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1538 m
-(O&7%#C"*#\(,8%#,#+,!,5,/%#,+,0!".B#C"*#,.%#.%,+C#!"#.%!.'%8%#+,!,:##E"&7%0!*,--CB#!\(%.%#,.%#!9"#$,'	,C/#!"#?"#,5"*!)[ 31.898003 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 34.194004 22.837000 9.143001 22.837000 11.398002 29.643002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 ] xS
-48 1588 m
-(.%!.'%8'&?#,&+#$,&'0*-,!'&?#?%&"$%#+,!,:##P'/!".'7,--CB#+,!,#\(,/#5%%&#\(,&+-%+#"&#,#7-"&%Q#5CQ#7-"&%#5,/'/:##;\(%)[ 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 29.643002 9.143001 20.500002 11.398002 22.837000 13.694001 9.143001 20.500002 22.837000 9.143001 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 20.500002 13.694001 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 ] xS
-48 1638 m
-(1&/1234#<=>#'/#\)*--C#7,0,5-%#"\)#9".G'&?#9'!\(#!\(%#7-"&%Q#5,/%+#+,!,#!\(,!#7"$%/#"*!#"\)#,#/%R*%&7'&?#0."6%7!B#,&+)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 9.143001 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 34.194004 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 22.837000 ] xS
-48 1688 m
-(\)*&+,$%&!,--C#1&/1234#9".G/#9'!\(#+,!,#,!#,#7"&!'?#-%8%-:##P"9%8%.B#+*%#!"#!\(%#&%,.'&?#7"$0-%!'"&#"\)#!\(%#\)'./!#5'?)[ 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 20.500002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 29.643002 22.837000 13.694001 20.500002 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 9.143001 22.837000 20.500002 22.837000 9.143001 11.398002 11.398002 11.398002 29.643002 22.837000 29.643002 22.837000 20.500002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 9.143001 22.837000 ] xS
-48 1738 m
-(?%&"$%#/%R*%&7'&?#0."6%7!/#'!#'/#&"9#5%7"$'&?#0"//'5-%#!"#0.,7!'7,--C#9".G#9'!\(#?%&"$%/#,/#-"&?#/!.%!7\(%/#"\)#?,0Q-%//)[ 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 29.643002 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 20.500002 9.143001 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 22.837000 9.143001 9.143001 20.500002 11.398002 29.643002 22.837000 13.694001 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 20.500002 11.398002 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 13.694001 9.143001 22.837000 20.500002 20.500002 ] xS
-48 1788 m
-(/%R*%&7%:##1&/1234#,-/"#,--"9/#C"*#!"#9".G#9'!\(#/%R*%&7%#'&\)".$,!'"&#'&#!\('/#9,C:##=.%8'"*/#.%-%,/%/#"\)#!\(%#1&/1234)[ 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 29.643002 22.837000 13.694001 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 29.643002 22.837000 20.500002 11.398002 11.398002 11.398002 27.347002 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 ] xS
-48 1838 m
-(<=>#7,--%+#!\(%/%#*&Q'&!%..*0!%+#/!.%!7\(%/#"\)#/%R*%&7%#8'.!*,-#7"&!'?/:##@."$#.%-%,/%#S#"&9,.+/#!\(%/%#\(,8%#5%%,--%+)[ 27.347002 27.347002 11.398002 11.398002 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 13.694001 9.143001 22.837000 11.398002 22.837000 13.694001 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 11.398002 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 9.143001 13.694001 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 25.051003 13.694001 22.837000 34.194004 11.398002 13.694001 22.837000 9.143001 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 29.643002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 ] xS
-48 1888 m
-(K-'7%/#Q##-'!%.,--CB#/-'7%/#"\)#?%&"$'7#/%R*%&7%:##<--#C"*#&%%+#!"#+"#!"#.%!.'%8%#/*7\(#,#/%R*%&7%#'/#/!'0*-,!%#!\(%#7\(."$/"$%)[ 27.347002 9.143001 9.143001 20.500002 22.837000 20.500002 11.398002 13.694001 11.398002 11.398002 9.143001 9.143001 11.398002 22.837000 13.694001 22.837000 9.143001 9.143001 20.500002 11.398002 11.398002 20.500002 9.143001 9.143001 20.500002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 27.347002 9.143001 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 20.500002 11.398002 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 20.500002 22.837000 34.194004 22.837000 ] xS
-48 1938 m
-(&*$5%.#,&+#!\(%#/!,.!#,&+#%&+#7"".+'&,!%/#Q##9%F--#?%!#!"#+"'&?#!\('/#'*/!#,#5'!:)[ 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 13.694001 11.398002 11.398002 29.643002 22.837000 7.831001 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 ] xS
-48 2038 m
-(2"/!#*/%./#"\)#!\(%#1&/1234#<=>#9'--#0."5,5-C#%&+#*0#*/'&?#5"!\(#$%,&/#"\)#.%!.'%8'&?#,&+#$,&'0*-,!'&?#?%&"$%)[ 34.194004 22.837000 20.500002 11.398002 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 34.194004 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 ] xS
-48 2088 m
-(/%R*%&7%/:##P%&7%B#9%F--#'&!."+*7%#5"!\(#"\)#!\(%/%#\(%.%#'&#!\(%#&%A!#!9"#/%7!'"&/:)[ 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 29.643002 22.837000 7.831001 9.143001 9.143001 11.398002 9.143001 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 ] xS
-/F4.1[ 41 0 0 -41 0 0]sf
-48 2244 m
-(,-\(."%&+.*&/+0,\(.#1'%)[ 29.643002 11.398002 25.051003 25.051003 22.837000 22.837000 11.398002 22.837000 25.051003 25.051003 11.398002 29.643002 22.837000 31.898003 29.643002 25.051003 25.051003 13.694001 11.398002 25.051003 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2344 m
-(E-"&%/#,&+#T,9E"&!'?/#.%0.%/%&!#!9"#5,/'7#+,!,Q!C0%/#'&/1234:##E-"&%/#\(,8%#7"&!'?/B#,&+#)[ 29.643002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 11.398002 29.643002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-1800 2344 m
-(7"&!'?/#,.%#!\(%#%-%$%&!/)[ 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 ] xS
-48 2394 m
-("\)#7"&!'?*"*/#/%R*%&7%#'&\)".$,!'"&#!\(,!#,.%#5*'-!#*0#!"#7.%,!%#!\(%#\)*--#7-"&%#/%R*%&7%:##O&7%#,#7-"&%#/%R*%&7%#'/)[ 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 13.694001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 31.898003 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 20.500002 ] xS
-48 2444 m
-(7"$0-%!%B#!\(%#7-"&%#9'--#5%#.%0.%/%&!%+#5C#"&%#7"&!'?#/%R*%&7%:)[ 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-48 2544 m
-(>\)#C"*#G&"9#!\(%#,77%//'"&#&*$5%.#"\)#,#7-"&%#!\(,!#C"*#,.%#'&!%.%/!%+#'&B#C"*#7,&#.%!.'%8%#!\(,!#7-"&%#+'.%7!-C#\)."$#!\(%)[ 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 ] xS
-48 2594 m
-(E-"&%<+,0!".#'&#!\(%#\)"--"9'&?#9,CL)[ 29.643002 9.143001 22.837000 22.837000 22.837000 27.347002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 20.500002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2689 m
-(%&'\(89-\)"'/'\(89-\)"$*+*,#-.12;"#8<$0&$*88"==>-\)5?4:@@ABBC?67)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2790 m
-(;\('/#9'--#.%!*.&#,#E-"&%#"56%7!:##U"*#7,\(%7G#9\(%!\(%.#C"*#\(,8%#!\(%#.'?\(!#7-"&%#5C#7,--'&?#!\(%#E-"&%#"56%7!F/#'+#$%!\("+L)[ 25.051003 22.837000 9.143001 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 29.643002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 20.500002 11.398002 29.643002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 9.143001 22.837000 22.837000 11.398002 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 29.643002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 7.831001 20.500002 11.398002 9.143001 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2881 m
-(,.>\)#'D:9-\)"'>='D'E'\(89-\)"12>+'E'DF\)D7)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2982 m
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-48 3032 m
-(7"&!'?/I:)[ 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 13.694001 11.398002 ] xS
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-/F1.1[ 41 0 0 -41 0 0]sf
-48 595 m
-(5&6.2%7#!"#$%&'\(\)*#89$%&'\(\)*+:#;)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 641 m
-(##!"#$-.'*\(7#,#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-441 641 m
-(%&'\(\)*/0-.'*\(74)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 687 m
-(##!"#$\)<#####,#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-441 687 m
-(%&'\(\)*/0\)<4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 779 m
-(##=#>7.#+.?@.'%.#&5#2#%&'\(\)*#\)+#&A\(2\)'.<#.2+\)-"B)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 871 m
-(##C6\)'\(#$%&'\(\)*/0+.?#D#EF'E4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 963 m
-(#=#G.#%2'#*.\(#2#+@A+\(6\)'*#&5#\(7\)+#+.?@.'%.#\(&&B)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1055 m
-(#C6\)'\(#$%&'\(\)*/0+@A+.?8HIHJJ:#D#EF'E4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1147 m
-(##=#K5#L.#L2'\(#\(&#L6\)\(.#\(7.#+.?@.'%.#\(#5\)-.#L.#@+.#A\)&C.6-#2*2\)')[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1193 m
-(##=#8M&\(.B#\)5#L.#26.#%6.2\(\)'*#2#'.L#&AN.%\(#L.#'..<#\(&#\)'%-@<.)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1239 m
-(##=#2#@+.#O\)&BBP.?KQ#-\)'.#2\(#\(7.#+\(26\(#&5#&@6#5\)-.)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1331 m
-(##@+.#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1331 m
-(O\)&BBP.?KQ4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1423 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1423 m
-(+.?\)&#,#'.L#O\)&BBP.?KQ8/57#####,0#FRP>SQT>I)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1469 m
-(############################/5&6!2\(##,0#U52+\(2U:4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1561 m
-(##$+.?\)&/0L6\)\(.1+.?8$%&'\(\)*:4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1653 m
-(V)s
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1757 m
-(@\($8#+"\(-7*"0#,\($#4"#%4-\(/$";#+*%<#\(#1\(&2%$-/36##A$"#0"-#%+#+"\(-7*"0#-'\(-#&/..#9*%\)"#"B-*"<".8#70"+7.#/+#8%7C*"#3%/$3#-%)[ 34.194004 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 11.398002 31.898003 22.837000 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 13.694001 22.837000 34.194004 22.837000 9.143001 20.500002 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 7.831001 13.694001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 ] xS
-48 1807 m
-(;%#\($8#\($\(.80/0#%$#-'"<#\(*"#-'"#*"9"\(-#+"\(-7*"06##D'"0"#,\($#4"#70";#-%#<\(0E#-'"#0":7"$,"#*"\(;8#+%*=#0\(8=#\(#FGH>D)[ 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 34.194004 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 20.500002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 ] xS
-48 1857 m
-(0"\(*,'6##I%*#"B\(<9."?)[ 20.500002 22.837000 22.837000 13.694001 20.500002 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 13.694001 11.398002 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1952 m
-(!"#$!2+W.<+.?#,#$%&'\(\)*/0*.\(16.C.2\(!2+W.<1+.?4)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2053 m
-(J"$,"=#K$0K@FG#9*%\)/;"0#8%7#&/-'#\(#'\($;8#,\(..#-%#%4-\(/$#*"9"\(-L<\(0E";#0":7"$,"#&/-'%7-#8%7#'\(\)/$3#-%#*"*7$)[ 29.643002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 34.194004 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 13.694001 22.837000 22.837000 ] xS
-48 2103 m
-(1"9"\(-@\(0E"*6)[ 29.643002 22.837000 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 20.500002 22.837000 13.694001 11.398002 ] xS
-48 2203 m
-(A+#,%7*0"=#3/\)"$#\(#&'%."#3"$%<"#0":7"$,"#-%#9.\(8#&/-'=#8%7#\(*"#'\(*;.8#3%/$3#-%#4"#,%$-"$-#&/-'#.%%E/$3#\(-#570-#%$")[ 31.898003 11.398002 11.398002 20.500002 22.837000 22.837000 13.694001 20.500002 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 22.837000 22.837000 11.398002 22.837000 11.398002 29.643002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 ] xS
-48 2253 m
-(,.%$"6##J"$,"=#-%#;%#3"$%<"#0/M";#\($\(.80"0#8%7#&/..#9*%4\(4.8#&\($-#-%#*"-*/"\)"#\(..#-'"#,.%$"0#+*%<#-'"#;\(-\(4\(0"6##F"+%*")[ 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 20.500002 9.143001 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 9.143001 20.500002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 11.398002 22.837000 13.694001 22.837000 ] xS
-48 2303 m
-(3%/$3#+7*-'"*#/-#/0#4"0-#-%#9%/$-#%7-#-'\(-#7$."00#8%7#'\(\)"#\($#%\)"*L*/;/$3#*"\(0%$#-%#&%*E#%$#\(#,.%$"L48L,.%$"#4\(0/0=#-'/0#E/$;)[ 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 13.694001 13.694001 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 29.643002 22.837000 13.694001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 13.694001 22.837000 20.500002 13.694001 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 9.143001 22.837000 22.837000 ] xS
-48 2353 m
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-48 2403 m
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-48 2498 m
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-48 2695 m
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-48 2787 m
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-48 2879 m
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-48 2925 m
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-48 2971 m
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-14 dict begin/FontName /BFXQWL+Courier def
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-/PaintType 0 def
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-/FontType 1 def
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-/Encoding 256 array 0 1 255{1 index exch/.notdef put}for
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-dup 33 /dollar put
-
-dup 34 /d put
-
-dup 35 /b put
-
-dup 36 /f put
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-dup 37 /e put
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-dup 38 /t put
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-dup 39 /c put
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-dup 40 /h put
-
-dup 41 /underscore put
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-dup 42 /a put
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-dup 43 /l put
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-dup 44 /parenleft put
-
-dup 45 /parenright put
-
-dup 46 /g put
-
-dup 47 /C put
-
-dup 48 /o put
-
-dup 49 /n put
-
-dup 50 /i put
-
-dup 51 /s put
-
-dup 52 /space put
-
-dup 53 /m put
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-dup 54 /y put
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-dup 55 /p put
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-dup 56 /r put
-
-dup 57 /equal put
-
-dup 58 /hyphen put
-
-dup 59 /greater put
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-dup 60 /S put
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-dup 61 /A put
-
-dup 62 /semicolon put
-
-dup 63 /quotesingle put
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-dup 64 /one put
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-dup 65 /comma put
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-dup 66 /zero put
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-dup 67 /E put
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-dup 68 /N put
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-dup 69 /G put
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-dup 70 /nine put
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-dup 71 /eight put
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-dup 72 /five put
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-dup 73 /six put
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-dup 74 /three put
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-dup 75 /period put
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-dup 76 /two put
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-readonly def
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-currentdict currentfile eexec
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-48 262 m
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-48 312 m
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-48 461 m
-(!"#$%&'#'\(\))[ 27.347002 22.837000 22.837000 15.990001 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 ] xS
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-48 575 m
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-48 674 m
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-/F3.1[ 41 0 0 -41 0 0]sf
-314.57999 674 m
-(!"#)[ 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-389 674 m
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-1264.17 674 m
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-1534.8199 674 m
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-48 725 m
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-48 825 m
-(J8'K\(\)#'$,'#\(&'\)+&-\)4&'325*&-'"<'-\)0'%"3#$4,'.&-'%/"3&'<"-'#\(&'<$-,#'ALL'%/"3&,'$3'#\(&';\)#\)*\),&D)[ 22.837000 11.398002 11.398002 38.704006 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 13.694001 22.837000 29.643002 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 13.694001 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 22.837000 ] xS
-48 925 m
-('''EC$3#F')[ 11.398002 11.398002 11.398002 13.694001 29.643002 9.143001 22.837000 11.398002 11.398002 11.398002 ] xS
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-191.52 925 m
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-/F1.1[ 41 0 0 -41 0 0]sf
-586 925 m
-("3'%/"3&IM)[ 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 13.694001 11.398002 ] xS
-48 1026 m
-(N8'O-$3#'"2#'$3'<\),#\)'<"-5\)#'#\(&'-&.&\)#>5\),:&;',&62&3%&'<"-'#\(&'/\),#'AL'%/"3&,'"<'#\(&'4&3"5&8)[ 22.837000 11.398002 11.398002 27.347002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 34.194004 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 ] xS
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-48 1176 m
-(*+&%#')[ 27.347002 11.398002 11.398002 22.837000 22.837000 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1276 m
-(P<'1"2'\(\)+&'\(\);'&@.&-$&3%&'0$#\('2,$34'#\(&'Q3,QGRS'TOP'*&<"-&7'1"2'0$//'\(\)+&'%"5&'\)%-",,'#\(&'%"3%&.#'"<'+$-#2\)/'%"3#$4,8)[ 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 13.694001 9.143001 22.837000 22.837000 20.500002 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 20.500002 13.694001 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 20.500002 9.143001 13.694001 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 ] xS
-48 1326 m
-(T'+$-#2\)/'%"3#$4'0\),'\)3')[ 27.347002 11.398002 20.500002 9.143001 13.694001 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 29.643002 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 ] xS
-469 1326 m
-(Q3,QGRS'"*?&%#'#\(\)#'-&.-&,&3#&;'\)',&%#$"3'"<'\)'4&3"5&8''U\(&,&'+$-#2\)/'%"3#$4,'%"2/;'*&'%-&\)#&;)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 9.143001 13.694001 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 ] xS
-48 1376 m
-(#"'-&.-&,&3#'\)31',$34/&'.\)-#'"<'#\(&'4&3"5&7'\)3;'0&-&'%-&\)#&;',$5./1'*1'5\):$34'\)'%\)//'#"'#\(&'V;*'"*?&%#',.&%$<1$34',#\)-#)[ 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 20.500002 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 13.694001 22.837000 11.398002 20.500002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 34.194004 22.837000 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 34.194004 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 11.398002 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 ] xS
-48 1426 m
-(\)3;'&3;'%""-;$3\)#&,''<"-'\)'.\)-#$%2/\)-'%\(-"5","5&8''U\(&'3$##1'4-$##1'"<'#\):$34'#\(&'\)%#2\)/'-\)0'%"3#$4',&62&3%&,'\)3;',#$#%\($34)[ 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 20.500002 22.837000 9.143001 22.837000 13.694001 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 20.500002 11.398002 22.837000 13.694001 9.143001 11.398002 11.398002 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 9.143001 11.398002 13.694001 22.837000 29.643002 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 11.398002 9.143001 11.398002 20.500002 22.837000 9.143001 22.837000 22.837000 ] xS
-48 1477 m
-(#\(&,&'#"4&#\(&-'$3#"'\)',$34/&',&62&3%&'0\),';"3&'$3'#\(&'*\)%:4-"23;'*1'#\(&'Q3,QGRS'%";&8''U\(&'%2--&3#'$3%\)-3#$"3'"<'\))[ 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 29.643002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 20.500002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 20.500002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 ] xS
-48 1527 m
-(+$-#2\)/'%"3#$4'$,'#\(&'=/$%&'>'+$-#2\)/'%"3#$4,'\)-&'3"'/"34&-'.\)-#'"<'#\(&'Q3,QGRS'%";&8''W2,#'/$:&'+$-#2\)/'%"3#$4,'\)'=/$%&'$,'\)3)[ 20.500002 9.143001 13.694001 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 13.694001 11.398002 20.500002 9.143001 13.694001 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 20.500002 22.837000 20.500002 11.398002 11.398002 9.143001 9.143001 20.500002 22.837000 11.398002 20.500002 9.143001 13.694001 11.398002 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 ] xS
-48 1577 m
-(Q3,QGRS'"*?&%#'#\(\)#'-&.-&,&3#,'\)31'.\)-#$%2/\)-'-&4$"3'"<'\)'4&3"5&8''T'=/$%&'\)3;'$#,'\),,"%$\)#&;'5&#\(";,'.-"+$;&'\)',$5./&)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 20.500002 22.837000 9.143001 22.837000 13.694001 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 11.398002 11.398002 27.347002 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 20.500002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 9.143001 34.194004 22.837000 9.143001 22.837000 ] xS
-48 1627 m
-(0\)1'#"'-&#-$&+&'\)3;'5\)3$.2/\)#&',&62&3%&'<-"5'\)'.\)-#'"<'#\(&'4&3"5&'#\(\)#'1"2'\)-&'$3#&-&,#&;'$38''U"'4&#'\)'=/$%&'"<'4&3"5&)[ 29.643002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 ] xS
-48 1677 m
-(1"2'?2,#'3&&;'#"';"'#\(&'<"//"0$348)[ 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 ] xS
-48 1777 m
-(X$-,#7'4&#'\)'=/$%&'\);\).#"-'<-"5'#\(&';\)#\)*\),&'\);\).#"-F)[ 25.051003 9.143001 13.694001 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1872 m
-(564!3+2'%\)*"*7&08494!"#:;.%&\)<+2'%="*7&08>)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1973 m
-(K$#\('\)'=/$%&'\);\).#"-7'-&#-$&+$34'4&3"5&',&62&3%&'$,'\),'&\),1'\),',.&%$<1$34'0\($%\('-&4$"3'"<'0\($%\('%\(-"5","5&'0&'\)-&)[ 38.704006 9.143001 11.398002 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 11.398002 20.500002 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 ] xS
-48 2023 m
-(\)<#&-F)[ 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2118 m
-(564!3+2'%494!3+2'%\)*"*7&08:;$%&'\(\)#6\)'\(8\)3&*8&\)%1",?@?A@A@BBBBB->)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2219 m
-(U\(&'"-;&-'#\(\)#'#\(&'\)-425&3#,'\)-&'.\),,&;'$,'%\(-"5","5&'325*&-7',#\)-#'%""-;$3\)#&'\)3;'&3;'%""-;$3\)#&8''!"#$%&'#\(\)#'#\($,'$,)[ 25.051003 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 ] xS
-48 2269 m
-(-&</&%#&;'$3'#\(&'3\)5$34'"<'#\(&',/$%&'\);\).#"-'5&#\(";8''Y&3&-\)//1'#\($,'$,'\)'4"";'42$;&'#"'42&,,$34'0\(\)#'\)-425&3#,'1"2'3&&;)[ 13.694001 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 31.898003 22.837000 22.837000 22.837000 13.694001 22.837000 9.143001 9.143001 20.500002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 20.500002 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 ] xS
-48 2319 m
-(#"'.-"+$;&'\)'5&#\(";'%\)//'>''\)/#&-3\)#$+&/1'1"2'%\)3'\(\)+&'\)'/"":'\)#'#\(&';"%25&3#\)#$"3'\),,"%$\)#&;'0$#\('\)'.\)-#$%2/\)-'5";2/&8)[ 11.398002 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 13.694001 11.398002 11.398002 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 20.500002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 20.500002 22.837000 9.143001 22.837000 13.694001 11.398002 34.194004 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 ] xS
-48 2419 m
-(T3"#\(&-'2,&<2/'0\)1'#"'"*#\)$3'\)'=/$%&'$,'#"';"'$#'0$#\('-&,.&%#'#"'\)'4&3&'$#'%"3#\)$3,F)[ 27.347002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 11.398002 29.643002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 13.694001 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2514 m
-(564!3+2'%494!3+2'%\)*"*7&08:;$%&'\(\)#6\).%1%\)3&*#+%\)2",?CD<EBBBBBBFFGGF?A4HBBB->)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2615 m
-(U\($,'0$//'--3'\)'=/$%&'#\(\)#'%"3#\)$3,'#\(&',&62&3%&'"<'#\(&'4&3&',.&%$<$&;'*1'$#,',#\)*/&'Q3,QGRS'$;8''P#'\)/,"'--3,'ZLLL*.)[ 25.051003 22.837000 9.143001 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 9.143001 11.398002 20.500002 11.398002 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 ] xS
-48 2665 m
-("<'</\)3:$34',&62&3%&'\)#'*"#\('#\(&'Z['\)3;'N['&3;,7'0\($%\('$,'"*+$"2,/1'+&-1'2,&<2/'$<'1"2'\)-&'$3#&-&,#&;'$3'#\(&'&3+$-"3,'#\(\)#'\))[ 22.837000 11.398002 11.398002 11.398002 9.143001 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 7.831001 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 7.831001 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 20.500002 22.837000 13.694001 20.500002 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 13.694001 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 ] xS
-48 2715 m
-(4&3&'$3\(\)*$#,8''\\"2'3&&;3[#'\(\)+&'#\(&'</\)3:$34',&62&3%&'$#'1"2';"3[#'0\)3#'$#'>''$3'#\($,'%\),&',&#'#\(&'325*&-'"<'</\)3:$34'*\),&,)[ 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 22.837000 9.143001 11.398002 20.500002 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 9.143001 11.398002 11.398002 13.694001 11.398002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 11.398002 9.143001 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 20.500002 ] xS
-48 2765 m
-(#"'L'"-'"5$#'#\(&',&%"3;'\)-425&3#'&3#$-&/18)[ 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 34.194004 9.143001 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 13.694001 22.837000 9.143001 20.500002 11.398002 ] xS
-48 2868 m
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-48 2918 m
-(%"3#$4,F)[ 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 3013 m
-(564!3+2'%494!3+2'%\)*"*7&08:;$%&'\(\)#6\)'01&2.\)1*5%,?=/BBHIIJKLK@K@BJ@LL?A4@BBBB->)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
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-/Encoding 256 array 0 1 255{1 index exch/.notdef put}for
-
-dup 33 /m put
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-dup 34 /y put
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-dup 35 /space put
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-dup 36 /dollar put
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-dup 37 /s put
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-dup 38 /l put
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-dup 39 /i put
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-dup 40 /c put
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-dup 41 /e put
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-dup 42 /equal put
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-dup 43 /underscore put
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-dup 44 /a put
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-dup 45 /d put
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-dup 46 /p put
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-dup 47 /t put
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-dup 48 /o put
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-dup 49 /r put
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-dup 50 /hyphen put
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-dup 51 /greater put
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-dup 52 /f put
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-dup 53 /h put
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-dup 54 /b put
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-dup 55 /n put
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-dup 56 /parenleft put
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-dup 57 /quotesingle put
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-dup 58 /A put
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-dup 59 /C put
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-dup 60 /zero put
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-dup 61 /five put
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-dup 62 /six put
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-dup 63 /three put
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-dup 64 /comma put
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-dup 65 /one put
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-dup 66 /parenright put
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-dup 67 /semicolon put
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-dup 68 /q put
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-dup 69 /u put
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-dup 70 /quotedbl put
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-dup 71 /S put
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-dup 72 /slash put
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-dup 73 /E put
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-dup 74 /backslash put
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-dup 75 /g put
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-dup 76 /at put
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-dup 77 /braceleft put
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-dup 78 /bracketleft put
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-dup 79 /bracketright put
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-dup 80 /quotedblleft put
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-dup 81 /period put
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-dup 82 /braceright put
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-readonly def
-
-/FontMatrix [ 0.00048828125 0 0 0.00048828125 0 0 ] def
-
-/FontBBox{-334 -839 1523 1793}def
-
-/UniqueID 4288209 def
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-currentdict currentfile eexec
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-48 212 m
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-48 262 m
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-48 362 m
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-48 457 m
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-48 558 m
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-48 608 m
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-48 708 m
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-1059 708 m
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-48 758 m
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-48 853 m
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-48 954 m
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-48 1052 m
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-48 1098 m
-(!"#$\(51+%/,1/##*#$%&'\(\)23\(51+%/,1/C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1144 m
-(!"#$\(51+\)7-####*#$%&'\(\)23\(51+\)7-C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1236 m
-(.1'7/#F;510!0%0!\)#$\(51+7,!\)#G/,1/HI7-#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1007 1236 m
-(\(51+%/,1/#$\(51+\)7-#J7FC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1333 m
-(!"'%7+<'\)2%/3%/\)&%,-#.'%#$%4'\('&7#*'4%56%\("'%+$$'75-6%H/&%\("'%0/-4'*%\(#-#*0%2+\("I%/3%/\)&%0'*/7'8%%93%1'%1+*\('4%\(/%+..'$$)[ 25.051003 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 13.694001 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 34.194004 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 34.194004 22.837000 9.143001 20.500002 11.398002 13.694001 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 22.837000 34.194004 22.837000 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 ] xS
-48 1383 m
-(\("#$%+$$'75-6%#*3/&7+\(#/*%1#\("%&'0+&4%\(/%+%2+&\(#.\)-+&%,-#.'%1'%.+*%4/%\("#$%56?)[ 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 20.500002 20.500002 22.837000 34.194004 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 20.500002 22.837000 9.143001 22.837000 13.694001 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1474 m
-(!"#$/'&'7K+.,/5#*#$%&'\(\)23)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-687 1474 m
-(K\)/+/'&'7K+.,/58BC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1566 m
-(401\),\(5#!"#$/'&\)#8L$/'&'7K+.,/5B#M)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1612 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1612 m
-(/'&\)+%&'\(\)#*#$/'&\)23,%%\)!6&\)-+G\)D8B237,!\)C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1658 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1658 m
-(/'&\)+\(/K###*#$/'&\)23\(0!.07\)7/+G\)D8B237,!\)C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1750 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1750 m
-(%&'\(\)+%/,1/#*#$/'&\)23,%%\)!6&\)-+%/,1/8BC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1796 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1796 m
-(%&'\(\)+\)7-###*#$/'&\)23,%%\)!6&\)-+\)7-8BC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1842 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1842 m
-(\(/K+%/,1/###*#$/'&\)23\(0!.07\)7/+%/,1/8BC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1888 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1888 m
-(\(/K+\)7-#####*#$/'&\)23\(0!.07\)7/+\)7-8BC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1934 m
-(##!"#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 1934 m
-(\(/K+01'#####*#$/'&\)23\(0!.07\)7/+01'8BC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2026 m
-(##.1'7/#FN$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-318 2026 m
-(/'&\)+%&'\(\)O#$%&'\(\)+%/,1/#2#$%&'\(\)+\)7-#*3#P#Q)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-109 2072 m
-(##PN$/'&\)+\(/KO#$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-502 2072 m
-(\(/K+%/,1/#2#$\(/K+\)7-#8$\(/K+01'BJ7FC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2118 m
-(R)s
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2219 m
-(J+."%K\(#-'K%\("+\(%1'%+&'%&'\(\)&*'4%#$%+%E#/??J*$JLEM??!#-'%/5G'.\(%&'2&'$'*\(#*0%\("'%2/&\(#/*%/3%+%$-#.'%\("+\(%7+2$%4#&'.\(-6%/*\(/%+)[ 27.347002 22.837000 20.500002 22.837000 11.398002 7.831001 11.398002 9.143001 9.143001 22.837000 7.831001 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 27.347002 9.143001 22.837000 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 25.051003 9.143001 9.143001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 9.143001 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 34.194004 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 ] xS
-48 2269 m
-(2/&\(#/*%/3%\("'%+$$7'5-6%H/&%0/-4'*%2+\("I8)[ 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 34.194004 22.837000 22.837000 9.143001 20.500002 11.398002 13.694001 22.837000 13.694001 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 ] xS
-48 2369 m
-(N%,-#.'%#$%+%0//4%$\(+&\(#*0%2-+.'%3/&%&'\(&#'A#*0%#*3/&7+\(#/*%+5/\)\(%+%&'0#/*%/3%+%."&/7/$/7'@%#*.-\)4#*0%\("'%0'*'$%#\(%./*\(+#*$8)[ 27.347002 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 11.398002 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 ] xS
-48 2420 m
-(!"'%*'O\(%3'1%$'.\(#/*$%1#--%0/%#*\(/%7/&'%4'\(+#-%+5/\)\(%"/1%\(/%4/%\("#$8)[ 25.051003 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 29.643002 11.398002 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2572 m
-(!"#$%&'\(&'\)*+,-./#0&)[ 27.347002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 31.898003 29.643002 25.051003 25.051003 13.694001 11.398002 25.051003 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2675 m
-(E'3/&'%0/#*0%/*%\(/%\("'%*'O\(%\(/2#.$%+*%#72/&\(+*\(%2/#*\(%*''4$%\(/%5'%7+4'8%%P'$2#\('%\("'%3+.\(%\("+\(%,-#.'$%+*4%C+1D/*\(#0$%+&')[ 27.347002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 9.143001 20.500002 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 20.500002 22.837000 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 ] xS
-48 2725 m
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-/F2.1[ 41 0 0 -41 0 0]sf
-48 312 m
-(!"#$%&'\(#!\)*#+,#-%.%/&01/23%4#2%56%7'%#,-+1#&\(%#'+7&89#+,#:+6-#'\(+8'%##;<87&=#>-%/&%#/##?@8'%#62879)[ 22.837000 11.398002 11.398002 25.051003 22.837000 11.398002 20.500002 22.837000 11.398002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 34.194004 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 9.143001 20.500002 22.837000 11.398002 11.398002 13.694001 29.643002 9.143001 22.837000 11.398002 11.398002 11.398002 29.643002 13.694001 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 361 m
-(!"#$%&'\(\)$"&*+&$%,&-#.,#&"/012$%,32-#.,#32"/04)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1179.79 361 m
-(#/74#\(/A%#/#@++3#*/'3#/&#\(+B#&\(82#B/2#4+7%#B8&\()[ 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 29.643002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 29.643002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 ] xS
-48 412 m
-(C/B>+7&892#87#&\(%#.-%A8+62#2%'&8+7D")[ 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 13.694001 11.398002 ] xS
-48 512 m
-(E"##$%&'\(#/#?@8'%#+,#&\(%#9%7%#FG?HIIIII!IIEJK)[ 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 20.500002 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 27.347002 29.643002 27.347002 31.898003 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 ] xS
-48 612 m
-(L"##M-87&#&\(%#@/2&#!III#76'@%+&84%2#+,#&\(%#?8@'%#+*&/87%4#87#&\(%#.-%A8+62#56%2&8+7")[ 22.837000 11.398002 11.398002 11.398002 27.347002 13.694001 9.143001 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 ] xS
-48 712 m
-(L"##$+-#&\(%#?@8'%#:+6#-%&-8%A%4#87#&\(%#,8-28%2&8+7N#.-87&#&\(%#842#+,#&\(%#'+7&892#&\(/&#,+-1#&\(%#&8@879#./&\("##O@2+#.-87&#&\(%#2&/-&)[ 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 9.143001 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 13.694001 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 27.347002 9.143001 20.500002 22.837000 11.398002 22.837000 13.694001 9.143001 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 11.398002 ] xS
-48 762 m
-(/74#%74#.+28&8+72#+,#%/'\(#C/B>+7&89#/2#8R#62%4#87#&\(%#&8@879#./&\(")[ 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 9.143001 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 20.500002 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 924 m
-(*#+,#-%#\(.#/0,$#')[ 27.347002 22.837000 25.051003 25.051003 22.837000 25.051003 22.837000 22.837000 11.398002 25.051003 22.837000 22.837000 13.694001 25.051003 15.990001 22.837000 22.837000 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1038 m
-(?+#7+B#B%P-%#.-%&&:#\(/..:#/*+6&#-%&-8%A879#QGO#2%56%7'%#,-+1#F72F\)RS"##T\(%#1+-%#87&%-%2&879#&\(8792#/22+'8/&%4#B8&\()[ 27.347002 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 29.643002 22.837000 7.831001 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 29.643002 29.643002 27.347002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 ] xS
-48 1088 m
-(?@8'%2#/74#C/B>+7&892#/-%#&\(%#,%/&6-%2#&\(/&#'/7#*%#-%&-8%A%4#,-+1#&\(%1"##T\(%2%#87'@64%#&\(%#-%.%/&#,%/&6-%2#1%7&8+7%4#87)[ 27.347002 9.143001 9.143001 20.500002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 34.194004 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 ] xS
-48 1138 m
-(.-%A8+62@:#/74#/@2+#2818@/-8&:#,%/&6-%2#;@83%#RSO?T#-%26@&2DN#.-%48'&8+7#&-/72'-8.&2#;26'\(#/2#9%72'/7#-%26@&2D#/74#1/-3%-)[ 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 9.143001 34.194004 9.143001 9.143001 22.837000 13.694001 9.143001 11.398002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 13.694001 9.143001 9.143001 20.500002 22.837000 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 11.398002 13.694001 22.837000 20.500002 22.837000 9.143001 11.398002 20.500002 13.694001 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 13.694001 20.500002 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 13.694001 22.837000 20.500002 22.837000 9.143001 11.398002 20.500002 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 13.694001 20.500002 22.837000 13.694001 ] xS
-48 1188 m
-(,%/&6-%2"##G+&#&+#1%7&8+7#9%7%2N#+,#'+6-2%")[ 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 11.398002 29.643002 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 34.194004 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 13.694001 20.500002 22.837000 11.398002 ] xS
-48 1288 m
-(?%&2#+,#,%/&6-%2#'/7#*%#-%&-8%A%4#,-+1#/#-%98+7#&\(-+69\(#/#-%56%2&#&+#/#?@8'%#+-#C/B>+7&89#;:+6P-%#.-+*/*@:#9%&&879#62%4#&+)[ 27.347002 22.837000 11.398002 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 13.694001 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 13.694001 20.500002 22.837000 22.837000 7.831001 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 ] xS
-48 1338 m
-(&\(82#*:#7+BD"##T\(%2%#/-%#-%&6-7%4#&+#62#/2#,%/&6-%#U+*V%'&2U"##$+-#872&/7'%=)[ 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 29.643002 13.694001 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 15.990001 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 15.990001 11.398002 11.398002 11.398002 25.051003 22.837000 13.694001 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1429 m
-(5+62,"7".#-6862-\(\)$"9:;"#&.\(\(&<"7".#=".#>,"-?)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1530 m
-(W%#7+B#\(/A%#/#-%,%-%7'%#&+#/#@82&#'+7&/87879#,%/&6-%#+*V%'&2"##O7#%/2:#B/:#&+#.-87&#&\(%2%#+6R#&+#'/@@#&\(%#1%&\(+4)[ 38.704006 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 11.398002 27.347002 22.837000 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 29.643002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 13.694001 9.143001 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1579 m
-(;!!-#,\)/;)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-269.436 1579 m
-(#B\(8'\(#-%&6-72#87,+-1/&8+7#/*+6&#&\(%#,%/&6-%#/2#/#2&-879=)[ 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 20.500002 11.398002 13.694001 9.143001 22.837000 22.837000 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1679 m
-(!@,".$%65+62,"7".#61A2,"7".#-46B)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1725 m
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-810 1725 m
-(/D?)[ 24.641003 24.641003 24.641003 ] xS
-48 1771 m
-(F)s
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-48 1872 m
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-/F3.1[ 41 0 0 -41 0 0]sf
-48 1967 m
-(GGHIJKLMGMN9IJLKOIGO)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-559 1967 m
-(<"7".#P.-Q",)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1009 1967 m
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-1909 1967 m
-(9I)[ 24.641003 24.641003 ] xS
-48 2013 m
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-559 2013 m
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-1009 2013 m
-(,"7".#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
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-(GLOL)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-1459 2013 m
-(TGJM)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-1609 2013 m
-(GMJJ)[ 24.641003 24.641003 24.641003 24.641003 ] xS
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-1909 2013 m
-(9I)[ 24.641003 24.641003 ] xS
-48 2059 m
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-559 2059 m
-(<"7".#P.-Q",)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1009 2059 m
-(,"7".#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1309 2059 m
-(NMMK)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-1459 2059 m
-(NMRJ)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-1609 2059 m
-(GTN)[ 24.641003 24.641003 24.641003 ] xS
-1759 2059 m
-(9)s
-1909 2059 m
-(9I)[ 24.641003 24.641003 ] xS
-48 2105 m
-(GGHIJKLMGMN9IJLKOIGO)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-559 2105 m
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-1009 2105 m
-(,"7".#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1309 2105 m
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-1459 2105 m
-(NTNR)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-1609 2105 m
-(IOJK)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-1759 2105 m
-(9)s
-1909 2105 m
-(9I)[ 24.641003 24.641003 ] xS
-48 2151 m
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-559 2151 m
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-1009 2151 m
-(,"7".#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1309 2151 m
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-1459 2151 m
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-1609 2151 m
-(RNL)[ 24.641003 24.641003 24.641003 ] xS
-1759 2151 m
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-1909 2151 m
-(9I)[ 24.641003 24.641003 ] xS
-48 2197 m
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-559 2197 m
-(<"7".#P.-Q",)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1009 2197 m
-(,"7".#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1309 2197 m
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-1459 2197 m
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-1609 2197 m
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-1909 2197 m
-(9I)[ 24.641003 24.641003 ] xS
-48 2243 m
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-559 2243 m
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-1009 2243 m
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-559 2289 m
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-1009 2289 m
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-48 2390 m
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-48 2490 m
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-48 2540 m
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-48 2590 m
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-48 2690 m
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-/F1.1[ 41 0 0 -41 0 0]sf
-48 936 m
-(:.;*#/*\(&0\)*'#<7%4*#=9>#%'7\(+,'#/.\)#%+'&\(+3*?#\(\)*#'%;97*#/*\(&0\)*'#\(+,#.+78#$\(@*#&$*#;*&$.,'#9\)%+&*,#\(5.@*A)[ 27.347002 22.837000 34.194004 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 13.694001 9.143001 9.143001 20.500002 22.837000 11.398002 29.643002 22.837000 31.898003 11.398002 9.143001 20.500002 9.143001 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 13.694001 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 13.694001 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 9.143001 34.194004 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-48 1036 m
-(B&$*\)#/*\(&0\)*'#\('#2*77#\('#$\(@%+1#3..\),%+\(&*'#.+#\(#3.+&%1#'*C0*+3*#\(7'.#$\(@*#3..\),%+\(&*'#.+#\(#$%&#'*C0*+3*D)[ 31.898003 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 9.143001 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1131 m
-("*%+,-.\(/0\(1%+2+,$\(341%+2+,$#5)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-109 1177 m
-(2%&'$\(7C&$\(#$,%$\(7\(9\(1%+2+,$:;.#$,%$\(9\(7='7>)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-109 1223 m
-(2%&'$\(7C&$\(+'A\(\(\(7\(9\(1%+2+,$:;.+'A\(9\(7=)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1068 1223 m
-('7>)[ 24.641003 24.641003 24.641003 ] xS
-48 1269 m
-(B)s
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1373 m
-(E*9*\(&#/*\(&0\)*'#\(\)*#\(+#*F\(;97*#./#\(#/*\(&0\)*#2$%3$#\(7'.#'&.\)*'#&$*#3..\),%+\(&*'#./#\(#$%&#'*C0*+3*A##G.2*@*\)H#&$*#37\(''%3)[ 29.643002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 29.643002 22.837000 20.500002 22.837000 13.694001 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 22.837000 20.500002 20.500002 9.143001 20.500002 ] xS
-48 1423 m
-(*F\(;97*#%'#\(#/*\(&0\)*#\)*9\)*'*+&%+1#\(#IJK:L#\)*'07$*\)*#&$*#C0*\)8#'*C0*+3*#%'#'%;%7\(\)#&.#\(+.&$*\)#'*C0*+3*#<9\).&*%+H)[ 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 11.398002 13.694001 22.837000 20.500002 22.837000 9.143001 11.398002 11.398002 29.643002 22.837000 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 20.500002 11.398002 20.500002 9.143001 34.194004 9.143001 9.143001 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 ] xS
-48 1473 m
-(M:LH#3NOK?#\(+,#'.#2*#+**,#&.#)[ 27.347002 27.347002 25.051003 11.398002 11.398002 20.500002 29.643002 29.643002 27.347002 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 ] xS
-630 1473 m
-('&.\)*#2$%3$#'*C0*+3*#%&#$\('#$%&#\(+,#2$*\)*\(5.0&#%+#&$\(&#'*C0*+3*#%&#$\('#$%&A##L$*'*#&89*')[ 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 11.398002 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 ] xS
-48 1523 m
-(./#/*\(&0\)*'#$\(@*#&$*#1*+*\)%3#+\(;*#./#'%;%7\(\)%&8#/*\(&0\)*'A##-+#\)*\(7%&8#&$*'*#/*\(&0\)*'#\(\)*#;*;5*\)'#./#\(#'09*\)37\(''#3\(77*,)[ 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 9.143001 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 9.143001 34.194004 9.143001 9.143001 22.837000 13.694001 9.143001 11.398002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 9.143001 11.398002 20.500002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 34.194004 22.837000 34.194004 22.837000 22.837000 13.694001 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 13.694001 20.500002 9.143001 22.837000 20.500002 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 22.837000 22.837000 ] xS
-48 1573 m
-(K7%1+P*\(&0\)*'#<5*3\(0'*#&$*8#\(\)*#/*\(&0\)*'#2%&$#&2.#\(7%1+*,#'*C0*+3*'?H#\(+,#,*9*+,%+1#.+#2$*&$*\)#%&#%'#NOK#.\)#9\).&*%+)[ 27.347002 9.143001 9.143001 22.837000 22.837000 25.051003 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 13.694001 22.837000 22.837000 20.500002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 29.643002 29.643002 27.347002 11.398002 22.837000 13.694001 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 ] xS
-48 1623 m
-('*C0*+3*#&$\(*#\(\)*#,*\(7%+1#2%&$H#&$*#.56*3&'#\(\)*#*%&$*\)#\(#I%.DDM+'MQIJDDN+\(N+\(K7%1+P*\(&0\)*#.\)#\()[ 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 27.347002 9.143001 22.837000 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 29.643002 22.837000 22.837000 27.347002 9.143001 9.143001 22.837000 22.837000 25.051003 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 ] xS
-48 1673 m
-(I%.DDM+'MQIJDDR\).&*%+N+\(K7%1+P*\(&0\)*)[ 27.347002 9.143001 22.837000 11.398002 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 27.347002 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 29.643002 22.837000 22.837000 27.347002 9.143001 9.143001 22.837000 22.837000 25.051003 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 ] xS
-48 1773 m
-(L.#1*&#'%;%7\(\)%&8#/*\(&0\)*'#/\).;#\(#E\(2=.+&%1#.\)#\(#'7%3*#2*#0'*D)[ 25.051003 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 9.143001 34.194004 9.143001 9.143001 22.837000 13.694001 9.143001 11.398002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 11.398002 20.500002 9.143001 9.143001 20.500002 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1868 m
-(/0\(1"+,$D%+#\(E\(1-*'$&!:;)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-639 1868 m
-(!+$F,GGF?&/&G,%&$0H+,$D%+#>)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1969 m
-(L.#.5&\(%+#.+78#N+\(K7%1+P*\(&0\)*'#2*#3\(+#0'*#&$*#/.77.2%+1#;*&$.,D)[ 25.051003 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 9.143001 20.500002 11.398002 29.643002 22.837000 22.837000 27.347002 9.143001 9.143001 22.837000 22.837000 25.051003 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2064 m
-(1"+,$D%+#\(E\(1#G&-+:;!+$F,GGFI',JG&!'H+,$D%+#3KL+%$%',KM\(N9NNNNO5>)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2165 m
-(O.&*#&$\(&#&$%'#;*&$.,#&\(4*'#&2.#.9&%.+\(7#\(\)10;*+&'#2$%3$#3\(+#%;9.'*#\(+#\(,,%&%.+\(7#3.+'&\)\(%+&#.+#&$*#/*\(&0\)*'#2*#2\(+&#&.)[ 29.643002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 34.194004 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 11.398002 9.143001 22.837000 22.837000 22.837000 9.143001 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 29.643002 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 ] xS
-48 2215 m
-(\)*&\)*%@*A##L$*#/%\)'&#\(\)10;*+&#%'#\(#S7.1%3#+\(;*S##T###&$*#+\(;*#/.\)#&$*#\(+\(78'%'#2$%3$#2\('#3.+,03&*,#&.#.5&\(%+#&$*#/*\(&0\)*A)[ 13.694001 22.837000 11.398002 13.694001 22.837000 9.143001 20.500002 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 7.831001 9.143001 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 7.831001 11.398002 11.398002 13.694001 11.398002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 29.643002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 ] xS
-48 2265 m
-(P.\)#'%;%7\(\)%&8#/*\(&0\)*'#&$*#7.1%3#+\(;*#\)*7\(&*'#&.#&$*#&89*#./#57\('&#\(+\(78'%'##\(+,#&$*\)*#\(\)*#30\)\)*+&78#/.0\)#7.1%3U+\(;*'#2$%3$)[ 25.051003 22.837000 13.694001 11.398002 20.500002 9.143001 34.194004 9.143001 9.143001 22.837000 13.694001 9.143001 11.398002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 11.398002 13.694001 22.837000 9.143001 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 22.837000 9.143001 20.500002 22.837000 22.837000 22.837000 34.194004 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 ] xS
-48 2315 m
-(3.\)\)*'9.+,#&.#,+\(U\(7%1+U/*\(&0\)*'#%+#&$*#30\)\)*+.,*#<+.&*#&$\(&#&$*#7.1%3#+\(;*#%'#=K:M#:MO:-L-VM?D)[ 20.500002 22.837000 13.694001 13.694001 22.837000 20.500002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 13.694001 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 11.398002 9.143001 20.500002 11.398002 29.643002 27.347002 27.347002 27.347002 11.398002 27.347002 27.347002 29.643002 27.347002 11.398002 25.051003 11.398002 27.347002 27.347002 13.694001 11.398002 ] xS
-48 2416 m
-(V*\)&\)+\()[ 27.347002 22.837000 13.694001 11.398002 13.694001 22.837000 22.837000 ] xS
-409 2416 m
-(WX#&57\('&+#$%&'#\(1\(%+'&#&$*#MQIJT)[ 38.704006 29.643002 11.398002 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 34.194004 27.347002 22.837000 13.694001 ] xS
-1034 2416 m
-(@*\)&\)+\(#,\(&\(5\('*#<\(77#=N:S'#/\).;#&$*#MQIJ#,\(&\(5\('*?A)[ 20.500002 22.837000 13.694001 11.398002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 9.143001 9.143001 11.398002 29.643002 29.643002 27.347002 7.831001 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 13.694001 11.398002 ] xS
-48 2466 m
-(:2\(77)[ 27.347002 29.643002 22.837000 9.143001 9.143001 ] xS
-409 2466 m
-(WX#57\('&9#$%&'#\(1\(%+'&#&$*#:WKJJ#,\(&\(5\('*#<9\).&*%+#/*\(&0\)*'#.+78?A)[ 38.704006 29.643002 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 38.704006 27.347002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 9.143001 20.500002 13.694001 11.398002 ] xS
-48 2516 m
-(X+%1*+*)[ 29.643002 22.837000 9.143001 22.837000 22.837000 22.837000 22.837000 ] xS
-259 2516 m
-(WX#&57\('&+#$%&'#\(1\(%+'&#&$*#X+%>*+*#,\(&\(5\('*A)[ 38.704006 29.643002 11.398002 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 9.143001 31.898003 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-48 2566 m
-(,5M:L)[ 22.837000 22.837000 27.347002 27.347002 25.051003 ] xS
-409 2566 m
-(WX#&57\('&+#$%&'#\(1\(%+'&#&$*#,5M:L#,\(&\(5\('*A)[ 38.704006 29.643002 11.398002 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 11.398002 22.837000 9.143001 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 27.347002 27.347002 25.051003 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-48 2666 m
-(G*+3*H#%+#.0\)#3\(77#&.#\)*&\)%*@*#NOK#/*\(&0\)*'H#2*#$\(@*#\)*C0*'&*,#&$.'*#&$\(&#\(\)*#$%&'#\(1\(%+'&#&$*#MQIJ#@*\)&\)+\(#,\(&\(5\('*A)[ 29.643002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 9.143001 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 29.643002 29.643002 27.347002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 22.837000 13.694001 11.398002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-48 2716 m
-(W*#$\(@*#\(7'.#'&%907\(&*,#\(#30&T#.//#@\(70*A##L$%'#\(77.2'#0'#&.#'*&#&$*#7*@*7#./#'&\)%1*+38#./#IJK:L#$%&'#\(+,#%'#*F\(3&78#&$*)[ 38.704006 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 11.398002 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 11.398002 13.694001 11.398002 22.837000 11.398002 11.398002 11.398002 20.500002 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 20.500002 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 22.837000 9.143001 11.398002 22.837000 11.398002 11.398002 20.500002 11.398002 13.694001 9.143001 22.837000 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 11.398002 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 11.398002 22.837000 9.143001 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 ] xS
-48 2766 m
-('\(;*#\('#&$*#IJK:L#M<"?#@\(70*A)[ 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 11.398002 27.347002 13.694001 22.837000 13.694001 11.398002 20.500002 22.837000 9.143001 22.837000 22.837000 11.398002 ] xS
-48 2866 m
-(B+3*#2*#$\(@*#&$*'*#NOK#/*\(&0\)*'#2*#3\(+#%&*\)\(&*#&$\).01$#&$*;#\(+,#&\(4*#\(#7..4#\($\(&#&$*8#3.+&\(%+D)[ 31.898003 22.837000 20.500002 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 29.643002 29.643002 27.347002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2961 m
-("*%+,-.\(/0\(1"+,$\(341"+,$D%+#5\(6)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 3007 m
-(\(\(2%&'$\(7H+,$D%+\(#-*%+#\(,%+\(7\(9\(1"+,$:;#-*%+\(\(\(\(\(\(9\(7=$7\(9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 3053 m
-(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(\(1"+,$:;2+%-+'$F&A\(9\(7=$7\(9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
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-48 207 m
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-48 253 m
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-48 354 m
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-/F1.1[ 41 0 0 -41 0 0]sf
-48 445 m
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-/F2.1[ 41 0 0 -41 0 0]sf
-48 546 m
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-48 596 m
-(\(\)*0#/2$4#&0-\)#%$"&1#$C##2$\)#0-\)2#,$;#$+&2$.**:$&0$0"#'$/2$0"#$%&'#$;&?$&%$;#$6-%0$,/,$4*\)$DEF$4#&0-\)#%A)[ 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 29.643002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 29.643002 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 29.643002 29.643002 27.347002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 ] xS
-48 696 m
-(F%$&2$&.0#\)2&0/1#$&\(\(\)*&+"$;#$+&2$3*$,/\)#+0.?$0*$0"#$*C6#+0$&,&\(0*\)$4*\)$&$4#&0-\)#$0?\(#$&2,$\)#G-#%0$4#&0-\)#%$4\)*'$0"#)[ 27.347002 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 13.694001 22.837000 20.500002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 ] xS
-48 746 m
-(,&0&C&%#A$$!"#$4*..*;/23$#H&'\(.#$/..-%0\)&0#%$%*'#$*4$0"#$,/44#\)#20$&\(\(\)*&+"#%$0"&0$+&2$C#$-%#,$0*$*C0&/2$4#&0-\)#%7)[ 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 9.143001 9.143001 9.143001 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 841 m
-(EF!"45E\),$*#$%&-9$*%G%\)&:9!H!"GI'\()[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-884 841 m
-(7$&*?5E\),$<$%&-9$;G%\)&:92)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 933 m
-(J7$&!%,,!:#!&K$!L\):151$!K5&4!:1!%!M:1&57)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 979 m
-(EF!N$\):151$*K5&4!H!NO"45E\),$*#$%&-9$*%G%\)&:9'\()[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1179 979 m
-(#$&6K*%,,*IF*P%@M:1&57="6:1&57A)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1025 m
-(>L\):151$>D32)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1117 m
-(J7$&!%,,!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-270 1117 m
-(M\)Q!54,%1G4!:1!%!?,56$!@5&K!46:9$!79$%&$9!&K%1!RBB)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1163 m
-(EF!"6\)7*54,%1G4!!H!"45E\),$*#$%&-9$*%G%\)&:9'\()[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1130 1163 m
-(#$&6K*%,,*IF*?,56$*%1G*46:9$="4,56$A!RBBA)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1209 m
-(>M\)Q>D2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1301 m
-(J7$&!&K$!45E\),$!#$%&-9$!@5&K!&K$!G%&%I%4$!5G$1&5#5$9!C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1347 m
-(EF!"45E\),$*#$%&-9$!H!"45E\),$*#$%&-9$*%G%\)&:9'\()[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1179 1347 m
-(#$&6K*IF*GIST=CD2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1439 m
-(J7$&!%,,!:#!&K$!45E\),$!#$%&-9$4!:1!%!4,56$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1485 m
-(EF!N45E\),$*#$%&-9$4!H!NO"4,56$'\(7$&*%,,*?5E\),$<$%&-9$432)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1577 m
-(J7$&!%,,!:#!&K$!&PU;46%1!K5&4!:1!%!6:1&57)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1623 m
-(EF!"&91%4!H!")[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-367 1623 m
-(6:1&57'\(7$&*%,,*?5E\),$<$%&-9$4=>&PU;46%1>D2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1769 m
-(!"#$%&'&\(\)*+,-.&)[ 29.643002 25.051003 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 31.898003 25.051003 11.398002 22.837000 22.837000 13.694001 22.837000 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1886 m
-(F..$4#&0-\)#%$\)#0\)/#1#,$4\)*'$0"#$,&0&C&%#$"&1#$&2$F2&.?%/%$*C6#+0$&00&+"#,$0*$0"#'$0"&0$+*20&/2%$/24*\)'&0/*2$\)#3&\),/23$0"#)[ 27.347002 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 1936 m
-(&2&.?%/%I&.3*\)/0"'$0"&0$+\)#&0#,$0"#$4#&0-\)#A$$!"/%$/%$&$3**,$'#&2%$C?$;"/+"$0*$,#0#\)'/2#$;"#\)#$0"#$4#&0-\)#$"&%$+*'#)[ 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 9.143001 22.837000 22.837000 13.694001 9.143001 11.398002 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 13.694001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 34.194004 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 34.194004 22.837000 ] xS
-48 1985 m
-(4\)*'$*\)$;"&0$/0$&+0-&..?$/%A$$!"#$)[ 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 13.694001 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 9.143001 11.398002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 9.143001 9.143001 20.500002 11.398002 9.143001 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-615.354 1985 m
-(7##*4:-96$!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-885.99799 1985 m
-('#0"*,$*4$0"#$F2&.?%/%$*C6#+0$0#..%$-%$;"&0$%*\)0$*4$4#&0-\)#$;#$"&1#5$%-+"$&%)[ 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 9.143001 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 ] xS
-48 2036 m
-(<3#2%+&2<5<\)#\(#&0<$*\)$<+\(3<A$$B0"#\)$/24*\)'&0/*2$+&2$C#$,#\)/1#,$4\)*'$0"#$F2&.?%/%$*C6#+05$%-+"$&%$0"#$,&0&C&%#$0"&0$;&%)[ 7.831001 22.837000 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 7.831001 11.398002 7.831001 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 7.831001 11.398002 22.837000 13.694001 11.398002 7.831001 20.500002 22.837000 22.837000 7.831001 11.398002 11.398002 11.398002 31.898003 11.398002 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 9.143001 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 20.500002 ] xS
-48 2086 m
-(-%#,$/2$&$JKF=!$6*C5$0"#$\(&\)&'#0#\)%$0"&0$;#\)#$\(\)*1/,#,$0*$0"#$#H#+-0&C.#5$&2,$#1#2$0"#$\(&0"$0*$0"#$#H#+-0&C.#$0"&0$;&%)[ 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 13.694001 22.837000 34.194004 22.837000 11.398002 22.837000 13.694001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 9.143001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 20.500002 ] xS
-48 2137 m
-(-%#,7)[ 22.837000 20.500002 22.837000 22.837000 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2232 m
-(EF!"%1%,F454!H!"#$%&-9$'\(%1%,F4542)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2324 m
-(\)951&!/;1%,F454V!!!!/!.!"%1%,F454'\(7##*4:-96$!.!/01/2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2370 m
-(\)951&!/T%&%I%4$!V!!!/!.!"%1%,F454'\(GI!!.!/01/2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2416 m
-(\)951&!/89:79%E!V!!!!/!.!"%1%,F454'\(\)9:79%E!.!/01/2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2462 m
-(\)951&!/8%9%E$&$94!V!/!.!"%1%,F454'\(\)%9%E$&$94!.!/01/2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2559 m
-(L*\)$/2%0&2+#$&$4#&0-\)#$0"&0$+*'#%$*-0$*4$&$JKF=!$\)-2$;/..$"&1#$&2$&2&.?%/%$*C6#+0$0"&0$0#..%$-%$/0$;&%$\)-2$;/0"$<C.&%0H<$&2,)[ 25.051003 22.837000 13.694001 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 34.194004 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 27.347002 22.837000 27.347002 27.347002 25.051003 11.398002 13.694001 22.837000 22.837000 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 9.143001 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 9.143001 11.398002 11.398002 29.643002 22.837000 20.500002 11.398002 13.694001 22.837000 22.837000 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 7.831001 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 7.831001 11.398002 22.837000 22.837000 22.837000 ] xS
-48 2609 m
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-dup 55 /h put
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-dup 56 /y put
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-dup 57 /exclam put
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-dup 58 /c put
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-dup 59 /d put
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-dup 60 /g put
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-dup 61 /period put
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-dup 62 /backslash put
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-dup 63 /semicolon put
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-dup 64 /braceright put
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-dup 65 /B put
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-dup 66 /m put
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-dup 67 /equal put
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-dup 68 /underscore put
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-dup 69 /G put
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-dup 70 /b put
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-dup 71 /at put
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-dup 72 /colon put
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-dup 73 /k put
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-dup 74 /w put
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-dup 75 /D put
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-dup 76 /L put
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-dup 77 /S put
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-readonly def
-
-/FontMatrix [ 0.00048828125 0 0 0.00048828125 0 0 ] def
-
-/FontBBox{-334 -839 1523 1793}def
-
-/UniqueID 4288209 def
-
-currentdict currentfile eexec
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-48 307 m
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-639 307 m
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-48 353 m
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-1450 353 m
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-48 399 m
-(!!!@!&.1&!3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 445 m
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-48 491 m
-(!!!@)[ 24.641003 24.641003 24.641003 24.641003 ] xS
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-48 591 m
-(7&%#)[ 25.051003 22.837000 22.837000 11.398002 ] xS
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-130.03999 591 m
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-351.47601 591 m
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-48 738 m
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-48 854 m
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-81 854 m
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-48 904 m
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-48 1007 m
-(I@)[ 22.837000 11.398002 ] xS
-81 1007 m
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-48 1057 m
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-48 1160 m
-(O@)[ 22.837000 11.398002 ] xS
-81 1160 m
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-48 1210 m
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-48 1359 m
-(*#+#')[ 31.898003 22.837000 25.051003 22.837000 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1473 m
-(K+,<0H#\)&%#5<5%=<0%#H%0%#C+<=4<0H#5,*E%--2#H%0%-#',%#C+<=\)#*0#Y=<E%-#C%E'+-%#'#=*\)#*"#H%0%-#-5'0##8*,%#\)&'0#*0%#)[ 29.643002 22.837000 13.694001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 11.398002 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-2092 1473 m
-(E*0\)<H@)[ 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 ] xS
-48 1523 m
-(F%0E%#2#<\)#8'B%-#\)&%#8*-\)#-%0-%#\)*#-+C-%D+%0\)=/#+-%#Y=<E%-#\)*#'EE%--#\)&%8@#F*$%\(%,2#&'\(<0H#-'<4#\)&'\)#/*+#E'0#'EE%--)[ 29.643002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 9.143001 11.398002 11.398002 34.194004 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 11.398002 29.643002 22.837000 29.643002 22.837000 20.500002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 ] xS
-48 1573 m
-(H%0%-#\(<'#Z'$Q*0\)<H-#<"#/*+#$<-&@##[%\)\)<0H#'==#\)&%#*"#\)&%#H%0%-#",*8#'#Y=<E%#*,#Z'$Q*0\)<H#<-#'-#%'-/#'-6)[ 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 20.500002 9.143001 22.837000 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 9.143001 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 29.643002 9.143001 20.500002 22.837000 11.398002 11.398002 11.398002 31.898003 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 13.694001 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 20.500002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1664 m
-(B8!%<&\)&1!C!%1.":&*+<&4D/..DE&\)&1?)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1765 m
-(M-#+-+'=#$%#',%#,%\)+,0%4#'#,%"%,%0E%#\)*#'#=<-\)#*"#*C\\%E\)-#.#[%0%#*C\\%E\)-#\)&<-#\)<8%@#7&%/#E*0\)'<0#'==#\)&%#<0"*,8'\)<*0#'C*+\))[ 27.347002 20.500002 11.398002 22.837000 20.500002 22.837000 22.837000 9.143001 11.398002 29.643002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 13.694001 11.398002 31.898003 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 11.398002 9.143001 34.194004 22.837000 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 ] xS
-48 1815 m
-(\)&%#%1*0]<0\),*0#-\),+E\)+,%2#KLM#-%D+%0E%2#%\)E@)[ 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 13.694001 22.837000 22.837000 11.398002 20.500002 11.398002 13.694001 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 29.643002 29.643002 27.347002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 11.398002 ] xS
-48 1914 m
-([%0%-#&'\(%#T0-TUVW#<4%0\)<"<%,-#$&<E&#E'0#C%#'EE%--%4#+-<0H#\)&%#)[ 31.898003 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 9.143001 22.837000 13.694001 20.500002 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1274.14 1914 m
-(14/F.&D";!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1520.1801 1914 m
-(8%\)&*46)[ 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2011 m
-(#\(-&/:7!B8!%<&\)&!$G%<&\)&12!3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2057 m
-(!!0-"\)4!5E&\)&!H!5!=!%)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-564 2057 m
-(<&\)&*+14/F.&D";!=!5>\)5?)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2103 m
-(@)s
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2204 m
-(T0-TUVW#<4%0\)<"<%,-#4*0?\)#,%'==/#\)%==#+-#8+E&#'C*+\)#\)&%#H%0%#9'04#\)&%/?,%#0*\)##<0\)%04%4#\)*;#'04#-*8%#H%0%-#$<==#&'\(%#*0%)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 9.143001 22.837000 13.694001 20.500002 11.398002 22.837000 22.837000 22.837000 7.831001 11.398002 11.398002 13.694001 22.837000 22.837000 9.143001 9.143001 20.500002 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 22.837000 20.500002 11.398002 34.194004 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 7.831001 13.694001 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 29.643002 9.143001 9.143001 9.143001 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 ] xS
-48 2254 m
-(*,#8*,%#8*,%#E*88*0#0'8%-@)[ 22.837000 13.694001 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 34.194004 34.194004 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 20.500002 11.398002 ] xS
-48 2353 m
-(J%#E'0#\)%==#<88%4<'\)%=/#<"#'#H%0%#<-#'#B0*$0#H%0%#9,%"-%D#*,#)[ 38.704006 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 9.143001 34.194004 34.194004 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 9.143001 20.500002 11.398002 9.143001 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 ] xS
-1155 2353 m
-(-5\),%8C=;#C/#E'==<0H#\)&%#)[ 20.500002 22.837000 11.398002 13.694001 22.837000 34.194004 22.837000 9.143001 13.694001 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1587.96 2353 m
-("1DI\)\(J\))[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1784.79 2353 m
-(#8%\)&*4@##!"#<\)#<-#'#B0*$0)[ 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 22.837000 ] xS
-48 2405 m
-(H%0%#\)&%0#$%#E'0#E'==#\)&%#%'E&XKVW<0B#8%\)&*4#\)*#"<04#*+\)#8*,%#'C*+\)#<\)@)[ 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 22.837000 29.643002 27.347002 22.837000 9.143001 22.837000 20.500002 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 9.143001 11.398002 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2496 m
-(#\(-&/:7!B8!%<&\)&!$G%<&\)&12!3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2542 m
-(!!"#!$%<&\)&*+"1DI\)\(J\)2!3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2588 m
-(!!!!!#\(-&/:7!B8!%."\)I!$G3%<&\)&*+<&4D/..DKAL"\)I1@2!3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2634 m
-(!!!!!!!0-"\)4!5E&\)&!5!=!%<&\)&*+14/F.&D";!=!5!)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1130 2634 m
-(."\)I1!4\(!5!=)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2680 m
-(!!!!!!!!!!!!!!!%."\)I*+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-590 2680 m
-(;"10./8D";!=!5!5!=)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2726 m
-(!!!!!!!!!!!!!!!%."\)I*+;/4/F/1&!=!5>\)5?)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2772 m
-(!!!!!!!B8!G)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-318 2772 m
-(18\)1!C!G3%."\)I*+<&4D/..D18\)\(\)8B1@?)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2818 m
-(!!!!!!!0-"\)4!5M8\)\(\)8B1!#\(-!<&\)&!/-&!G18\)1>\)5?)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2864 m
-(!!!!!@)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2910 m
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-(!!@)[ 24.641003 24.641003 24.641003 ] xS
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-/F2.1[ 41 0 0 -41 0 0]sf
-48 895 m
-(:\)%"4$;-+,-."<\)*-8=>.\(%4*#$%&'\(120"\(54665?<\)*#@9->)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 941 m
-(---'%&*\(-AB\)C*!-"<\)*-A-D-."<\)*12)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-836 941 m
-(#\(4E6"5&!----D-A-A-D)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 987 m
-(-------------------------."<\)*12#\(4%\(-D-A-)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1082 987 m
-(A-D)[ 24.641003 24.641003 24.641003 ] xS
-48 1033 m
-(-------------------------."<\)*12"*!---D-A-)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1082 1033 m
-(A-D)[ 24.641003 24.641003 24.641003 ] xS
-48 1079 m
-(-------------------------."<\)*12)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-836 1079 m
-(#"F12#"F3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1125 m
-(@)s
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1225 m
-(B*"'3$&"#,"&3,88'\(1&"#$&2$;&+$"#*4&*\(&,\(&?7*\(&*->$3"&%$".%\(2&.2&,&F'*G$%8&2$;.$\(3$&*->$3"5&&E$&"#$\(&#,9$&"*&3,88&)[ 29.643002 22.837000 11.398002 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 20.500002 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 27.347002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 27.347002 9.143001 22.837000 27.347002 22.837000 13.694001 9.143001 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 11.398002 11.398002 38.704006 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-2103.76 1225 m
-(#"F)[ 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1276 m
-(,1,'\(&"*&1$"&,&2"%'\(1&%$:%$2$\(","'*\(&*\)&"#$&2$;.$\(3$5&&H$\(3$I&0$&1$"&"#$&)[ 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 11.398002 13.694001 9.143001 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1360.86 1276 m
-(#"F12#"F-)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1582.3 1276 m
-(2".""$%&J&'"2&\(*"&,&"<:*&'\(&3,2$&<*.)[ 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 13.694001 11.398002 9.143001 11.398002 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 ] xS
-48 1327 m
-(0$%$&0*\(4$%'\(15)[ 29.643002 22.837000 13.694001 22.837000 11.398002 29.643002 22.837000 22.837000 22.837000 22.837000 13.694001 9.143001 22.837000 22.837000 11.398002 ] xS
-48 1426 m
-(E$&3,\(&1$"&"#$&:%*"$'\(&2$;.$\(3$&*\)&,&6%,\(23%':"&-<&3,88'\(1&"#$&)[ 38.704006 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 25.051003 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1185.25 1426 m
-(\(%4*#64\(")[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-1406 1426 m
-(&+$"#*45&&K*&"*&1$"&,88&"#$&:%*"$'\(&"%,\(28,"'*\(2)[ 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 ] xS
-48 1478 m
-(\)%*+&,&1$\($&'\("*&,&\)'8$&0$&0*.84&4*)[ 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 11.398002 9.143001 9.143001 22.837000 11.398002 29.643002 22.837000 11.398002 29.643002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1569 m
-(+,-.#"F&\)-/-*"G-)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-441 1569 m
-(H&\)IIJ"FKL1281:\)%+4\(-/2-M:4#\(4MN)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1615 m
-(-----------------------------1:;-----/2-OPJ7QLR793)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1707 m
-(:\)%"4$;-+,-.\(%4*#$%&'\(-8=>.0"*"120"\(546657%4*#$%&'\(#@9->)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1753 m
-(--+,-.'"'\(&!"-/-.\(%4*#$%&'\(12\(%4*#64\("3)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1845 m
-(--.#"F&\)12G%&\("5#"F8.'"'\(&!"93)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1891 m
-(@)s
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1988 m
-(B*"$&"#,"&0#$\(&0%'"'\(1&"*&,&F'*G$%8&)[ 29.643002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 22.837000 11.398002 29.643002 13.694001 9.143001 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 22.837000 27.347002 22.837000 13.694001 9.143001 11.398002 ] xS
-689 1988 m
-(K$;L!&*->$3"&0$&:,22&"#$&G$:"'4$&*->$3"&,\(4&\(*"&,&2"%'\(1I&-."&0#$\(&0%'"'\(1&*."&"*&"#$)[ 27.347002 22.837000 22.837000 11.398002 31.898003 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 11.398002 13.694001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 22.837000 22.837000 11.398002 29.643002 13.694001 9.143001 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 2038 m
-(23%$$\(&0$&#,9$&"*&2"%'\(1'\)<&'"&\)'%2"5)[ 20.500002 20.500002 13.694001 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 11.398002 13.694001 9.143001 22.837000 22.837000 9.143001 11.398002 20.500002 11.398002 9.143001 11.398002 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2187 m
-(!"#$%&'#'\(\))[ 27.347002 22.837000 22.837000 15.990001 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2251 m
-(M5&&=$"&,88&"#$&1$\($2&*\(&&,&8*\(1J&'2#&@2,<&MN-D&K8'3$&,\(4&:%'\("&"#$'%&?\(2?NFO&'425)[ 22.837000 11.398002 11.398002 11.398002 31.898003 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 13.694001 11.398002 9.143001 20.500002 22.837000 11.398002 13.694001 20.500002 22.837000 20.500002 11.398002 22.837000 34.194004 22.837000 13.694001 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 9.143001 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 9.143001 13.694001 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 9.143001 22.837000 20.500002 11.398002 ] xS
-48 2351 m
-(P5&&E#'3#&*\)&"#*2$&1$\($2&,%$&/\(*0\(&1$\($2&,\(4&0#,"&'2&"#$'%&+*%$&3*++*\(&\(,+$Q&&@H'\("5&R2$&"#$&'2S/\(*0\(&+$"#*4&,\(4)[ 22.837000 11.398002 11.398002 11.398002 38.704006 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 9.143001 13.694001 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 34.194004 34.194004 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 13.694001 29.643002 9.143001 22.837000 11.398002 11.398002 11.398002 29.643002 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 9.143001 20.500002 22.837000 20.500002 22.837000 22.837000 29.643002 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 ] xS
-48 2401 m
-(.2$&"#$&1$"S,88SAFO'\(/2JT4'2:8,<S'4D)[ 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 22.837000 29.643002 27.347002 22.837000 9.143001 22.837000 20.500002 20.500002 13.694001 23.944002 22.837000 9.143001 20.500002 22.837000 9.143001 22.837000 20.500002 22.837000 9.143001 22.837000 13.694001 ] xS
-48 2501 m
-(U5&&H*0&+,\(<&1$\($2&,%$&,8"$%\(,"'9$8<&2:8'3$4&\)*%&"#$&\)'%2"&MVV&1$\($2&&@H'\("W&X*.\("&"#$&\(.+-$%&*\)&"%,\(23%':"2&.2'\(1&"#$)[ 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 29.643002 11.398002 34.194004 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 9.143001 20.500002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 9.143001 9.143001 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 29.643002 9.143001 22.837000 11.398002 11.398002 11.398002 29.643002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 2551 m
-(Y1$\($JT$,3#S6%,\(23%':"&+$"#*4D)[ 22.837000 22.837000 22.837000 22.837000 22.837000 13.694001 23.944002 22.837000 22.837000 20.500002 22.837000 22.837000 25.051003 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 13.694001 ] xS
-48 2651 m
-(Z5&&E#,"&'2&"#$&,9$%,1$&2'[$&,\(4&\(.+-$%&*\)&$7*\(2&:$%&1$\($&&@H'\("W&R2$&"#$&)[ 22.837000 11.398002 11.398002 11.398002 38.704006 22.837000 22.837000 11.398002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 20.500002 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 11.398002 11.398002 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 13.694001 29.643002 9.143001 22.837000 11.398002 11.398002 11.398002 29.643002 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-1408 2651 m
-(1$"S,88S?7*\(2&+$"#*4&,\(4&%$+$+-$%&"#,")[ 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 9.143001 22.837000 27.347002 20.500002 22.837000 22.837000 20.500002 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 13.694001 22.837000 34.194004 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-48 2701 m
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-48 2801 m
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-48 2851 m
-(*\($&"%,\(23%':"&:$%&1$\($D)[ 22.837000 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 ] xS
-48 2951 m
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-48 3001 m
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-240 3001 m
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-48 212 m
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-48 312 m
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-48 411 m
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-48 525 m
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-1891 525 m
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-48 575 m
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-48 671 m
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-48 766 m
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-48 812 m
-(\(\(\)*\(+3#%-0ᆲ\(6\(+,$-$78,$39%::9;#%-0ᆲ<)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 858 m
-(\(\(\(!"#$%&'\(\)*\(+3#%-0Dž\(./+3#%-0냵\(2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 904 m
-(\(\(\(\(\(\)*\(+$="-0\(6\(+3#%-0녂,$39%::9>="-0<)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 950 m
-(\(\(\(\(\(\(!"#$%&'\(\)*\(+$="-\(./+$="-01\(2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 996 m
-(\(\(\(\(\(\(\(\(\(\)*\(+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-367 996 m
-(0?55"#34-,9!$%3?#$0\(6\(+$="-78,$39%::90?55"#34-,9!$%3?#$0<)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1042 m
-(\(\(\(\(\(\(\(\(\(!"#$%&'\(\)*\(+!$%3?#$\(./+0?55"#34-,9!$%3?#$01\(2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1088 m
-(\(\(\(\(\(\(\(\(\(\(\(\(5#4-3\(@>A4B$-&$\(@\(C\(+!$%3?#$78,!!03#4-,\(C\(@D)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1425 1088 m
-(-@<)[ 24.641003 24.641003 24.641003 ] xS
-48 1134 m
-(\(\(\(\(\(\(\(\(\(E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1180 m
-(\(\(\(\(\(\(E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1226 m
-(\(\(\(E)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1272 m
-(E)s
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1418 m
-(0%.-'/&'$\(*1%2\(3/%'#&3)[ 27.347002 15.990001 22.837000 25.051003 11.398002 22.837000 13.694001 11.398002 25.051003 25.051003 11.398002 25.051003 15.990001 22.837000 25.051003 22.837000 22.837000 15.990001 11.398002 25.051003 13.694001 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1531 m
-($.0$DEF#0&/',0#)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 ] xS
-/F1.1[ 41 0 8.7148399 -41 0 0]sf
-364.729 1531 m
-(\(9#-.-&-/#)[ 22.837000 22.837000 11.398002 9.143001 22.837000 9.143001 11.398002 9.143001 22.837000 11.398002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-518 1531 m
-(5,.,#L',6-\)&-/.0#1'/2#5,.0\)\(."##B+,0,#L',6-\)&-/.0#\(',#\(\)\),00,0#-.#\(#0-2-*\('#1\(0+-/.#&/#./'2\(*)[ 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 22.837000 11.398002 20.500002 9.143001 34.194004 9.143001 9.143001 22.837000 13.694001 11.398002 11.398002 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 34.194004 22.837000 9.143001 ] xS
-48 1581 m
-($.0$DEF#&'\(.0\)'-L&0#M#9:&#&+'/:5+#>',6-\)&-/.B'\(.0\)'-L&#/9J,\)&0#'\(&+,'#&+\(.#B'\(.0\)'-L&#/9J,\)&0"##N0#7-&+#./'2\(*#&'\(.0\)'-L&0)[ 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 27.347002 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 25.051003 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 25.051003 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 11.398002 27.347002 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 34.194004 22.837000 9.143001 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 ] xS
-48 1631 m
-(L',6-\)&-/.#&'\(.0\)'-L&0#\(',#\)/2L/0,6#/1#\(#0,&#/1#$%/.#/9J,\)&0"##B/#/9&\(-.#L',6-\)&-/.#&'\(.0\)'-L&0#1'/2#\(#O*-\),#/'#P\(7Q/.&-5)[ 22.837000 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 22.837000 20.500002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 27.347002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 13.694001 11.398002 29.643002 22.837000 29.643002 29.643002 22.837000 22.837000 11.398002 9.143001 22.837000 ] xS
-48 1681 m
-(7,#\)\(.#6/#&+,#1/**/7-.5#8\(0#7-&+#/&+,'#$.0$DEF#1,\(&:',0##*/5-\)#.\(2,#/'#0\)/',#\)/.0&'\(-.&0#\)\(.#9,#L\(00,6#\(0#/L&-/.\(*)[ 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 13.694001 22.837000 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 9.143001 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 34.194004 22.837000 11.398002 22.837000 13.694001 11.398002 20.500002 20.500002 22.837000 13.694001 22.837000 11.398002 20.500002 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 9.143001 22.837000 11.398002 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 22.837000 9.143001 ] xS
-48 1731 m
-(\('5:2,.&0<#K)[ 22.837000 13.694001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 13.694001 11.398002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1829 m
-(\)*\(+5#$B4&3$B93#%-0ᆲ\(6\(+0:4&$78)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-933 1829 m
-(,$39%::9F#$B4&34"-;#%-0ᆲ<)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1926 m
-(R,#./7#+\(;,#\(#',1,',.\),#&/#\(#*-0&#/1#>',6-\)&-/.B'\(.0\)'-L&#/9J,\)&0"##B/#+\(;,#\(#*//C#\(&#&+,#L',6-\)&,6#)[ 38.704006 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 27.347002 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 9.143001 22.837000 22.837000 25.051003 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 ] xS
-1827 1926 m
-(,%/.0#1/'#,\(\)+)[ 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 20.500002 22.837000 ] xS
-48 1976 m
-(L',6-\)&,6#&'\(.0\)'-L,#\)\(.#:0,#&+,#1/**/7-.5K)[ 22.837000 13.694001 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2067 m
-(!"#$%&'\(\)*\(+5#$B4&3$B93#%-0Dž\(./+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-933 2067 m
-(5#$B4&3$B93#%-0냵\(2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2113 m
-(\(\(\)*\(+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-195 2113 m
-($="-0\(6\(+5#$B4&3$B93#%-0녂)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-957 2113 m
-(,$39%::9>="-0<)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2212 m
-(\(\(5#4-3\(@G$-0&%-\(5#$B4&34"-\('%0\(@\(C\()[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-933 2212 m
-(0&%:%#\(/+$="-0\(C\(H\($="-0D-@<)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2304 m
-(\(\(!"#$%&'\(\)*\(+$="-\(./+$="-01\(2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2350 m
-(\(\(\(\(5#4-3\(+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-318 2350 m
-($="-7803%#3\(\(C\(@\(7\(@\(C)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2396 m
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-48 212 m
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-48 262 m
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-48 312 m
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-48 403 m
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-48 500 m
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-48 591 m
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-393 591 m
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-48 637 m
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-393 637 m
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-48 734 m
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-48 784 m
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-48 875 m
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-48 921 m
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-48 1017 m
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-130.03999 1017 m
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-277.664 1017 m
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-/F2.1[ 41 0 0 -41 0 0]sf
-358 1017 m
-(6\(7)[ 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-431.81201 1017 m
-(%3.,$20\)%'.1.'%,2%,$.%.E2\(%#22'0*\(",.\)%\)2%,$.F%\)$2:70%\(./.'%5.%7.\)\)%,$"\(%2\(.%2'%;'.",.'%,$"\(%,$.)[ 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 13.694001 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 ] xS
-48 1069 m
-(.E2\(9\)%7.\(;,$@)[ 22.837000 20.500002 22.837000 22.837000 7.831001 20.500002 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1218 m
-(!"#$%&')[ 27.347002 15.990001 25.051003 13.694001 22.837000 11.398002 25.051003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1331 m
-(G\(12'3",*2\(%'.;"'0*\(;%"%+'2,.*\(%#"\(%5.%'.,'.*/.0%1'23%,$.%)[ 11.398002 22.837000 11.398002 22.837000 13.694001 34.194004 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 13.694001 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 22.837000 9.143001 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-1088 1331 m
-(H'2,.*\(B0"+,2'%/*"%"%&'"\(\)7",*2\(%256.#,%*0.\(,*1*.'D)[ 27.347002 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 27.347002 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 20.500002 9.143001 22.837000 11.398002 22.837000 11.398002 25.051003 13.694001 22.837000 22.837000 20.500002 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 9.143001 22.837000 13.694001 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1427 m
-(!"#$/&,%6+\(3'7'/%,&#-#$780196%3:&,%6+\(;7'/%,&2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1519 m
-(!"#$/&,%6+\(##-#$/&,%6+\(3'7'/%,&01<6%.=38"3%&'\(\)*'%+,\(3+7>$%&'\(\)*'%+,\(0178?@A2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1620 m
-(B\)%./.'F%,'"\(\)#'*+,%$"\)%2\(7F%2\(.%,'"\(\)7",*2\(%C.%#"\(%1.,#$%+'2,.*\(\)%:\)*\(;%,$.%,'"\(\)#'*+,%*0.\(,*1*.'%"\)%C.77D)[ 27.347002 20.500002 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 9.143001 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 9.143001 22.837000 11.398002 9.143001 22.837000 22.837000 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 22.837000 20.500002 20.500002 13.694001 9.143001 22.837000 11.398002 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 9.143001 22.837000 13.694001 11.398002 22.837000 20.500002 11.398002 29.643002 22.837000 9.143001 9.143001 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1715 m
-(!"#$/&,%6+\(#-#$/&,%6+\(3'7'/%,&01<6%.=38"3%&'\(\).&+/%3+7>$%&'\(\).&+/%0178?@A)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1816 m
-(I\(#.%C.%$"/.%"%H'2,.*\(%256.#,%C.%#"\(%722J%",%*,\)%1.",:'.\))[ 31.898003 22.837000 20.500002 22.837000 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 27.347002 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 9.143001 11.398002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1911 m
-(!"#$/&,%6+\(3<6'%B&6\)#-#$/&,%6+\(0196%3'**3:&,%6+\(C6'%B&6\)2)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2007 m
-(&$.%1.",:'.\)%,$",%"'.%'.,:'\(.0%"'.%H'2,.*\(K.",:'.\)@%%&2%$"/.%"%722J%",%,$.3%F2:%#"\(%:\).%,$.%)[ 25.051003 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 27.347002 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 25.051003 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 20.500002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1729.96 2007 m
-(9<<3\)%&+\(9#)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-2000.6 2007 m
-(3.,$20D)[ 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2099 m
-(<,&6'.=#!"#$/<#>D$/&,%6+\(3<6'%B&6\)A#E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2145 m
-(####/&+\(%#$/<019<<\)%&+\(9#F#GH)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-761 2145 m
-(\(G2)[ 24.641003 24.641003 24.641003 ] xS
-48 2191 m
-(I)s
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2291 m
-(%\(%"\)&*%*+,)[ 22.837000 22.837000 22.837000 15.990001 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2405 m
-(L@)[ 22.837000 11.398002 ] xS
-81 2405 m
-(%%K*\(0%"77%,$.%+1"3%023"*\(\)%#2\(,"*\(.0%*\(%,$.%1*'\),%LMM%;.\(.\)%2\(%#$'232\)23.%L@%%N$*#$%2\(.\)%"'.%32\),%#2332\(%"\(0%*\))[ 11.398002 11.398002 25.051003 9.143001 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 34.194004 11.398002 22.837000 22.837000 34.194004 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 38.704006 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 34.194004 22.837000 20.500002 11.398002 11.398002 20.500002 22.837000 34.194004 34.194004 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 9.143001 20.500002 ] xS
-48 2455 m
-(,$*\)%\):'+'*\)*\(;O)[ 11.398002 22.837000 9.143001 20.500002 11.398002 20.500002 22.837000 13.694001 22.837000 13.694001 9.143001 20.500002 9.143001 22.837000 22.837000 22.837000 ] xS
-48 2555 m
-(P@)[ 22.837000 11.398002 ] xS
-81 2555 m
-(%%Q2C%3"\(F%21%,$2\).%;.\(.\)%$"/.%\(2%+'2,.*\(%1.",:'.\)%",%"77@)[ 11.398002 11.398002 29.643002 22.837000 29.643002 11.398002 34.194004 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 ] xS
-48 2655 m
-(?@)[ 22.837000 11.398002 ] xS
-81 2655 m
-(%%Q2C%3"\(F%21%,$.%+'2,.*\(\)%#2\(,"*\(%N=RM%023"*\(\)O)[ 11.398002 11.398002 29.643002 22.837000 29.643002 11.398002 34.194004 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 38.704006 29.643002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 9.143001 22.837000 20.500002 22.837000 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2804 m
-(-\($%"'./+0.$.1.*%*+2+34%+5%'%"./+67%.)[ 27.347002 22.837000 13.694001 22.837000 15.990001 25.051003 22.837000 11.398002 11.398002 29.643002 22.837000 13.694001 22.837000 25.051003 22.837000 22.837000 22.837000 22.837000 11.398002 13.694001 11.398002 25.051003 25.051003 22.837000 11.398002 31.898003 22.837000 25.051003 22.837000 15.990001 22.837000 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 2918 m
-(&$.%#2'.%!\(\)!STU%0","5"\).%V,$.%2\(.%F2:9/.%5..\(%:\)*\(;%:+%,2%\(2CW%#2\(,"*\(\)%=AB-%;.\(.\)%"\(0%\)23.%\).X:.\(#.%1.",:'.\)@)[ 25.051003 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 7.831001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 29.643002 13.694001 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 11.398002 29.643002 29.643002 27.347002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 ] xS
-48 2968 m
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-48 3068 m
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-48 262 m
-(;<=>'"\)\)+#"#1+\)%/''?+4'3"\)'\)+@'"33&%%'#\(&'-&"#4.&%'1\)'#\(&'%&3+\)!'!"#"$"%&'"%'#\(+46\('#\(&A'@&.&'"**'1\)'#\(&'%"5&'B*"3&)[ 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 20.500002 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 29.643002 22.837000 13.694001 22.837000 11.398002 22.837000 9.143001 9.143001 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 9.143001 22.837000 20.500002 22.837000 ] xS
-48 312 m
-(&C&\)'#\(+46\('#\(&A'3+4*!'$&'%1##1\)6'+\)'"'3+5B*&#&*A'!1--&.&\)#'5"3\(1\)&/)[ 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 20.500002 9.143001 11.398002 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 22.837000 9.143001 20.500002 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 11.398002 11.398002 34.194004 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 11.398002 ] xS
-48 412 m
-(>&#D%'61C&'#\(&';70'!"#"$"%&'"%'"'-1.%#'&2"5B*&/)[ 22.837000 22.837000 11.398002 7.831001 20.500002 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 27.347002 25.051003 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 9.143001 13.694001 20.500002 11.398002 11.398002 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 11.398002 ] xS
-48 512 m
-(E1.%#'@&'\)&&!'#+'3+\)\)&3#'#+'#\(&'5"1\)';\)%;<=>'!"#"$"%&F)[ 25.051003 9.143001 13.694001 20.500002 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 34.194004 22.837000 9.143001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 607 m
-(!"#$%&'\(\(\)*"\)+%,\(\(\)-.#/*0120.0\(\(\)3435,\(\(2%6708.'/9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 699 m
-(:;$<#".=7>$?$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-367 699 m
-(%&'\(\(\)*"\)+%,\(\(\)-.#/*0120.0\(\(\)3435,\(\(2%6708.'/@A*#BC)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 745 m
-($$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$$@D'".$$$?A$<D'".E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1009 791 m
-($$$$@7>*0:#$?A$<#".*0:#E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1009 837 m
-($$$$@!"#/$$$?A$<!"#/F9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 934 m
-(G\)!'#\(&\)'#+'#\(&'\(45"\)';70'!"#"$"%&'#+'+4.'5"1\)'!$'"!"B#+.'"\)!'C13&HC&.%"F)[ 27.347002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 27.347002 27.347002 25.051003 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 34.194004 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 9.143001 20.500002 22.837000 13.694001 20.500002 22.837000 13.694001 20.500002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1032 m
-(<7>@A077=7>=0708.'/CG#".GE$<#".=7>F9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1078 m
-(<#".)[ 24.641003 24.641003 24.641003 24.641003 ] xS
-147 1078 m
-(=7>@A077=7>=0708.'/CGH'/#GE$<7>F9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1175 m
-(8+@'#\("#'@&'\("C&'!+\)&'#\(1%'@&'3"\)'.&#.1&C&'#\(&';70'"*16\)5&\)#'-&"#4.&%'-.+5'#\(1%'#\(&'!"#"$"%&'1\)'#\(&'%"5&'@"A'"%'@&)[ 29.643002 22.837000 29.643002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 13.694001 22.837000 11.398002 13.694001 9.143001 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 27.347002 25.051003 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 34.194004 22.837000 11.398002 29.643002 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 29.643002 22.837000 ] xS
-48 1225 m
-(@+4*!'+#\(&.'I8G'"*16\)'-&"#4.&%'$A'%B&31-A1\)6'#\(&'\)"5&'+-'#\(&'"\)"*A%1%'#\("#'@"%'4%&!'#+'6&\)&."#&'#\(&'"*16\)5&\)#%'H'1\)'#\(1%)[ 29.643002 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 29.643002 29.643002 27.347002 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 11.398002 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 9.143001 20.500002 20.500002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 20.500002 11.398002 22.837000 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 9.143001 9.143001 22.837000 22.837000 34.194004 22.837000 22.837000 11.398002 20.500002 11.398002 13.694001 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 ] xS
-48 1275 m
-(3"%&'D&2J&K6J-&"#D/)[ 20.500002 22.837000 20.500002 22.837000 11.398002 7.831001 22.837000 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 7.831001 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1370 m
-(:;$<#".=I#0.!/#"$?$<"1&H#@AJ#.=011=2*061&J*K#0.!/#"CG#-=#LJ=I#0.GF9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1467 m
-(8+@'#\("#'@&'\("C&'#\(&%&'-&"#4.&%'@&'3"\)'\("C&'"'*++,'"#'#\(&5F)[ 29.643002 22.837000 29.643002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 29.643002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 29.643002 22.837000 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 34.194004 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1558 m
-(I'/#0HD$:;$<#".$CM<#".=I#0.!/#"F$N)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 1604 m
-($$$$8/&*.$O$O$P$<#".@AJII"./&*J$P$OQ)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-933 1604 m
-(*O9)[ 24.641003 24.641003 24.641003 ] xS
-48 1650 m
-(R)s
-/F3.1[ 41 0 0 -41 0 0]sf
-48 1800 m
-(!"#!)[ 27.347002 29.643002 27.347002 27.347002 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1914 m
-(789'-&"#4.&%'3"\)'$&'.&"!1*A'"33&%%&!'-.+5'#\(&'&2#&.\)"*'!"#"$"%&'&\)%&5$*H*1#&F)[ 27.347002 29.643002 27.347002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 13.694001 22.837000 22.837000 22.837000 9.143001 9.143001 20.500002 11.398002 22.837000 20.500002 20.500002 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 11.398002 22.837000 13.694001 22.837000 22.837000 9.143001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 13.694001 9.143001 9.143001 11.398002 22.837000 11.398002 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 2009 m
-(!"#$%&'\(\(\)*"\)+%,\(\(,&.#\(\(2%6708.'/9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2101 m
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-859 2147 m
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-859 2193 m
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-48 2294 m
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-/F2.1[ 41 0 0 -41 0 0]sf
-48 2389 m
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-48 2435 m
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-/F1.1[ 41 0 0 -41 0 0]sf
-48 2535 m
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-/F2.1[ 41 0 0 -41 0 0]sf
-993.72302 2535 m
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-/F1.1[ 41 0 0 -41 0 0]sf
-1288.97 2535 m
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-/F2.1[ 41 0 0 -41 0 0]sf
-48 2636 m
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-1007 2636 m
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-48 2738 m
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-48 2833 m
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-48 2879 m
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-318 2879 m
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-48 2925 m
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-(@4)[ 22.837000 11.398002 ] xS
-81 904 m
-($$A,..#-'$',$'"#$-,0#$B.+BCDE$\(\)'\)*\)+#$\).\($'"#$>FG$\(\)'\)*\)+#4$$H\(\($'"#$>FG$\(\)'\)*\)+#$',$'"#$B.+BCDE$\(\)'\)*\)+#)[ 11.398002 11.398002 29.643002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 20.500002 22.837000 13.694001 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 29.643002 27.347002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 29.643002 27.347002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 22.837000 ] xS
-48 954 m
-(\).\($6&-#96#0+\)4)[ 22.837000 22.837000 22.837000 11.398002 20.500002 9.143001 20.500002 22.837000 13.694001 20.500002 22.837000 13.694001 20.500002 22.837000 11.398002 ] xS
-48 1054 m
-(I4)[ 22.837000 11.398002 ] xS
-81 1054 m
-($$A0#\)'#$\)$>%&-#$,/$@C*$,/$+#J2#.-#$/0,1$8,20$-"0,1,+,1#$,/$-",&-#$K"&.'$9$0#1#1*#0$*\)-3$',$'"#$>%&-#$#L#0-&+#+M)[ 11.398002 11.398002 29.643002 13.694001 22.837000 22.837000 11.398002 22.837000 11.398002 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 20.500002 22.837000 22.837000 13.694001 11.398002 20.500002 22.837000 13.694001 22.837000 34.194004 22.837000 20.500002 22.837000 34.194004 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 9.143001 20.500002 22.837000 11.398002 13.694001 22.837000 9.143001 22.837000 11.398002 11.398002 13.694001 11.398002 13.694001 22.837000 34.194004 22.837000 34.194004 22.837000 22.837000 13.694001 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 9.143001 20.500002 22.837000 20.500002 13.694001 ] xS
-48 1154 m
-(N4)[ 22.837000 11.398002 ] xS
-81 1154 m
-($$O,7$1\).8$>FG+$\)0#$'"#0#$&.$'"&+$0#<&,.P)[ 11.398002 11.398002 29.643002 22.837000 29.643002 11.398002 34.194004 22.837000 22.837000 20.500002 11.398002 27.347002 29.643002 27.347002 20.500002 11.398002 22.837000 13.694001 22.837000 11.398002 11.398002 22.837000 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 22.837000 22.837000 ] xS
-48 1253 m
-(Q4)[ 22.837000 11.398002 ] xS
-81 1253 m
-($$O,7$1\).8$>FG+$,6#0%\):$#L,.+P$KO&.'$9$<#'$\)%%$<#.#+$/0,1$'"#$>%&-#$\).\($2+#$)[ 11.398002 11.398002 29.643002 22.837000 29.643002 11.398002 34.194004 22.837000 22.837000 20.500002 11.398002 27.347002 29.643002 27.347002 20.500002 11.398002 22.837000 20.500002 22.837000 13.694001 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 22.837000 20.500002 22.837000 11.398002 13.694001 29.643002 9.143001 22.837000 11.398002 11.398002 13.694001 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 9.143001 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-1500.72 1253 m
-(2\(34%--4:;<=\))[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-1820.58 1253 m
-($',$<#'$'"#$)[ 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-2015 1253 m
-(#L,.+$/0,1)[ 22.837000 20.500002 22.837000 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 34.194004 ] xS
-48 1304 m
-('"#$<#.#+4$R+#$'"#$)[ 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 29.643002 20.500002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-403.56699 1304 m
-(<>\(&-%?\))[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-601 1304 m
-($1#'",\($',$-\)%-2%\)'#$'"#$:0,:,0'&,.$,/$>FG+$'"\)'$\)0#$+&'2\)'#\($&.$-,\(&.<$+#J2#.-#+4M)[ 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 9.143001 20.500002 22.837000 9.143001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 13.694001 11.398002 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 27.347002 29.643002 27.347002 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 11.398002 20.500002 9.143001 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 11.398002 20.500002 22.837000 22.837000 9.143001 22.837000 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 13.694001 ] xS
-/F1.1[ 41 0 0 -41 0 0]sf
-48 1456 m
-(0&1.2"34&5\)\(&.678#\("2\(4&28\(%&\(8&\)*%#*&81&839%#&:%#*.82*&81&\(;%&+2*+!<=&0>?&@&=.*\(*&"29&=.*\(&A%1%#%2-%*)[ 29.643002 11.398002 13.694001 11.398002 25.051003 22.837000 11.398002 11.398002 11.398002 25.051003 25.051003 13.694001 11.398002 11.398002 36.490002 25.051003 25.051003 15.990001 13.694001 22.837000 25.051003 13.694001 11.398002 11.398002 25.051003 25.051003 13.694001 22.837000 11.398002 13.694001 25.051003 11.398002 25.051003 22.837000 22.837000 15.990001 22.837000 11.398002 25.051003 13.694001 11.398002 25.051003 11.398002 25.051003 22.837000 15.990001 11.398002 22.837000 22.837000 15.990001 22.837000 11.398002 25.051003 25.051003 22.837000 11.398002 25.051003 13.694001 11.398002 13.694001 25.051003 22.837000 11.398002 27.347002 25.051003 22.837000 27.347002 34.194004 29.643002 25.051003 11.398002 29.643002 27.347002 11.398002 11.398002 13.694001 11.398002 25.051003 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 25.051003 25.051003 11.398002 25.051003 11.398002 22.837000 13.694001 11.398002 29.643002 22.837000 13.694001 22.837000 15.990001 22.837000 25.051003 22.837000 22.837000 22.837000 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-48 1556 m
-(!"#$B.+BCDE$HGS$"\)+$T2+'$0#-#.'%8$:\)++#\($'"0,2<"$\)$0\):&\($:#0&,\($,/$-"\).<#4$$H$+&<.&/&-\).'$\(&//#0#.-#$*#'7##.$,%\($\).\()[ 25.051003 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 20.500002 11.398002 11.398002 13.694001 22.837000 20.500002 22.837000 22.837000 11.398002 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 13.694001 22.837000 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 13.694001 9.143001 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 27.347002 11.398002 20.500002 9.143001 22.837000 22.837000 9.143001 11.398002 9.143001 20.500002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 11.398002 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 22.837000 22.837000 11.398002 29.643002 22.837000 22.837000 22.837000 11.398002 22.837000 9.143001 22.837000 11.398002 22.837000 22.837000 22.837000 ] xS
-48 1606 m
-(.#7$6#0+&,.+$,/$'"#$HGS$&+$'"#$.,7$7&\(#+:0#\)\($2+#$,/$%&+'$0#/#0#.-#+$K%&+'0#/+M4$$>,1#$1#'",\($-\)%%+$-\).$:,'#.'&\)%%8$0#'20.)[ 22.837000 22.837000 29.643002 11.398002 20.500002 22.837000 13.694001 20.500002 9.143001 22.837000 22.837000 20.500002 11.398002 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 29.643002 11.398002 29.643002 9.143001 22.837000 22.837000 20.500002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 13.694001 9.143001 9.143001 20.500002 11.398002 13.694001 22.837000 11.398002 20.500002 13.694001 11.398002 11.398002 11.398002 27.347002 22.837000 34.194004 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 20.500002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 9.143001 22.837000 9.143001 9.143001 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 ] xS
-48 1656 m
-(6#08$%,.<$%&+'+$\).\($&'$&+$/\)0$1,0#$#//&-&#.';$*,'"$'&1#9$\).\($1#1,0897&+#;$/,0$'"#+#$1#'",\(+$',$0#'20.$0#/#0#.-#+$',$'"#+#)[ 20.500002 22.837000 13.694001 20.500002 11.398002 9.143001 22.837000 22.837000 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 11.398002 22.837000 13.694001 11.398002 34.194004 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 9.143001 20.500002 9.143001 22.837000 22.837000 11.398002 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 11.398002 11.398002 9.143001 34.194004 22.837000 13.694001 11.398002 22.837000 22.837000 22.837000 11.398002 34.194004 22.837000 34.194004 22.837000 13.694001 20.500002 13.694001 29.643002 9.143001 20.500002 22.837000 11.398002 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 20.500002 22.837000 ] xS
-48 1706 m
-(%&+'+$&.+'#\)\(4$$H%'",2<"$.,'$\)%%$1#'",\(+$0#'20.$%\)0<#$\)00\)8+$,/$,*T#-'+;$/,0$-,.+&+'#.-8$\)%1,+'$\)%%$1#'",\(+$&.$'"#$HGS$+",2%\()[ 9.143001 9.143001 20.500002 11.398002 20.500002 11.398002 9.143001 22.837000 20.500002 11.398002 22.837000 22.837000 22.837000 11.398002 11.398002 11.398002 27.347002 9.143001 11.398002 22.837000 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 22.837000 13.694001 22.837000 22.837000 11.398002 22.837000 13.694001 13.694001 22.837000 20.500002 20.500002 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 20.500002 11.398002 11.398002 11.398002 22.837000 13.694001 11.398002 20.500002 22.837000 22.837000 20.500002 9.143001 20.500002 11.398002 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 9.143001 34.194004 22.837000 20.500002 11.398002 11.398002 22.837000 9.143001 9.143001 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 ] xS
-48 1756 m
-(.,7$0#'20.$%&+'$0#/#0#.-#+$0\)'"#0$'"\).$%&+'+U\)00\)8+4$$H$.\)1&.<$+'\).\(\)0\($"\)+$\)%+,$*##.$\(#6#%,:#\($7"&-"$+",2%\($1\)3#$&')[ 22.837000 22.837000 29.643002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 22.837000 13.694001 11.398002 11.398002 22.837000 22.837000 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 20.500002 11.398002 22.837000 13.694001 13.694001 22.837000 20.500002 20.500002 11.398002 11.398002 11.398002 27.347002 11.398002 22.837000 22.837000 34.194004 9.143001 22.837000 22.837000 11.398002 20.500002 11.398002 22.837000 22.837000 22.837000 22.837000 13.694001 22.837000 11.398002 22.837000 22.837000 20.500002 11.398002 22.837000 9.143001 20.500002 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 22.837000 20.500002 22.837000 9.143001 22.837000 22.837000 22.837000 22.837000 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 20.500002 22.837000 22.837000 22.837000 9.143001 22.837000 11.398002 34.194004 22.837000 20.500002 22.837000 11.398002 9.143001 11.398002 ] xS
-48 1806 m
-(#\)+�$/,0$HGS$2+#0+$',$+201&+#$7"&-"$1#'",\(+$0#'20.$\)$+&.<%#$6\)%2#$,0$0#'20.$\)$%&+'$0#/#0#.-#4$$D2+&.#++$,*T#-'$1#'",\(+)[ 22.837000 22.837000 20.500002 9.143001 22.837000 13.694001 11.398002 11.398002 22.837000 13.694001 11.398002 27.347002 27.347002 11.398002 11.398002 22.837000 20.500002 22.837000 13.694001 20.500002 11.398002 11.398002 22.837000 11.398002 20.500002 22.837000 13.694001 34.194004 9.143001 20.500002 22.837000 11.398002 29.643002 22.837000 9.143001 20.500002 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 20.500002 9.143001 22.837000 22.837000 9.143001 22.837000 11.398002 20.500002 22.837000 9.143001 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 11.398002 27.347002 22.837000 20.500002 9.143001 22.837000 22.837000 20.500002 20.500002 11.398002 22.837000 22.837000 9.143001 22.837000 20.500002 11.398002 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 ] xS
-48 1855 m
-(7&'"$'"#$:0#/&L$)[ 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 20.500002 11.398002 ] xS
-/F4.1[ 41 0 8.7148399 -41 0 0]sf
-312.33801 1855 m
-(!"#$%&&$)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-/F2.1[ 41 0 0 -41 0 0]sf
-509.17001 1855 m
-($0#'20.$\)$%&+'$0#/#0#.-#;$\)+$\(,$\)\(\):',0$1#'",\(+$7&'"$'"#$:0#/&L)[ 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 20.500002 11.398002 22.837000 22.837000 11.398002 22.837000 22.837000 22.837000 22.837000 11.398002 22.837000 13.694001 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 20.500002 11.398002 29.643002 9.143001 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 11.398002 9.143001 20.500002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-1614.47 1855 m
-(#)s
-/F4.1[ 41 0 8.7148399 -41 0 0]sf
-1639.0699 1855 m
-('"#\(\)$%&&)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1860 1855 m
-($)s
-/F2.1[ 41 0 0 -41 0 0]sf
-1884.6 1855 m
-($4$$S/$8,2$\)0#)[ 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 11.398002 20.500002 22.837000 22.837000 11.398002 22.837000 13.694001 22.837000 ] xS
-48 1907 m
-(0#'20.#\($\)$%&+'0#/$/0,1$\)$1#'",\($-\)%%$&'$&+$\)$+&1:%#$1\)''#0$',$\(#90#/#0#.-#$'"&+4$$!"#$/,%%,7&.<$#L\)1:%#+$&%%2+'0\)'#$'"#$2+#$,/)[ 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 22.837000 11.398002 22.837000 11.398002 9.143001 9.143001 20.500002 11.398002 13.694001 22.837000 11.398002 11.398002 11.398002 13.694001 22.837000 34.194004 11.398002 22.837000 11.398002 34.194004 22.837000 11.398002 22.837000 22.837000 22.837000 11.398002 20.500002 22.837000 9.143001 9.143001 11.398002 9.143001 11.398002 11.398002 9.143001 20.500002 11.398002 22.837000 11.398002 20.500002 9.143001 34.194004 22.837000 9.143001 22.837000 11.398002 34.194004 22.837000 11.398002 11.398002 22.837000 13.694001 11.398002 11.398002 22.837000 11.398002 22.837000 22.837000 13.694001 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 11.398002 11.398002 22.837000 9.143001 20.500002 11.398002 11.398002 11.398002 25.051003 22.837000 22.837000 11.398002 11.398002 22.837000 9.143001 9.143001 22.837000 29.643002 9.143001 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 34.194004 22.837000 9.143001 22.837000 20.500002 11.398002 9.143001 9.143001 9.143001 22.837000 20.500002 11.398002 13.694001 22.837000 11.398002 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 22.837000 20.500002 22.837000 11.398002 22.837000 11.398002 ] xS
-48 1957 m
-(%&+'$0#/#0#.-#+$&.$'"#$B.+BCDE$HGS?)[ 9.143001 9.143001 20.500002 11.398002 11.398002 13.694001 22.837000 11.398002 22.837000 13.694001 22.837000 22.837000 20.500002 22.837000 20.500002 11.398002 9.143001 22.837000 11.398002 11.398002 22.837000 22.837000 11.398002 27.347002 22.837000 20.500002 27.347002 34.194004 27.347002 22.837000 11.398002 27.347002 27.347002 11.398002 11.398002 ] xS
-/F3.1[ 41 0 0 -41 0 0]sf
-48 2052 m
-(@A\(3/B#%--#/-<=\(\)#A&<!#3B\(#/-<=\(#%C%?3<&)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2098 m
-(!"#,/-<=\(\)#*#,/-<=\(4%C%?3<&01)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-761 2098 m
-(A\(3/B4%--9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1459 2098 m
-(@#&\(37&=\)#%#-.\)3#&\(A\(&\(=/\()[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2190 m
-(@.A#"<7#!7\)3#B%>\(#%#/<?"#<A#3B\(#&\(A\(&\(=/\(C#%&&%"D#C<#3B.\)E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2236 m
-(!"#$/-<=\(\)#*#$,/-<=\(\)9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-1459 2236 m
-(@#F73#"<7#C<=G3#=\(\(C#3<#C<#3B.\))[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2328 m
-(@2\(3#3B\(#A.&\)3#/-<=\(#A&<!#3B\(#-.\)3#>.%#3B\(#&\(A\(&\(=/\(E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2374 m
-(!"#,A.&\)34/-<=\(#*#,/-<=\(\)01HIJ9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2466 m
-(@%=<3B\(&#K%"#<A#2\(33.=2#3B\(#\)%!\(#3B.=2E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2512 m
-(L,A.&\)34/-<=\(M#*#$,/-<=\(\)9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2604 m
-(@.3\(&%3\(#3B&<72B#%--#<A#3B\(#/<=3.2\)#<=#%#/-<=\()[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2650 m
-(A<&\(%/B#!"#,/<=3.2#L$+,A.&\)34/-<=\(012\(34%--4N<=3.2\)8M#+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2696 m
-(##?&.=3#,/<=3.201=%!\(#O#PQ)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-687 2696 m
-(=R9)[ 24.641003 24.641003 24.641003 ] xS
-48 2742 m
-(8)s
-48 2834 m
-(@%=<3B\(&#K%"#<A#C<.=2#3B\(#\)%!\(#3B.=2E)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2880 m
-(!"#,/<=3.2\)#*#,A.&\)34/-<=\(012\(34%--4N<=3.2\)9)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2926 m
-(A<&\(%/B#!"#,/<=3.2#L$,/<=3.2\)M#+)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-48 2972 m
-(##?&.=3#,/<=3.201=%!\(#O#PQ)[ 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 24.641003 ] xS
-687 2972 m
-(=R9)[ 24.641003 24.641003 24.641003 ] xS
-48 3018 m
-(8)s
-ep
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diff --git a/docs/tutorial/ensembl_tutorial.txt b/docs/tutorial/ensembl_tutorial.txt
index b53f75fbbb..ea39079dfb 100644
--- a/docs/tutorial/ensembl_tutorial.txt
+++ b/docs/tutorial/ensembl_tutorial.txt
@@ -1,1080 +1,845 @@
-EnsEMBL Tutorial
-by Michele Clamp (and revised/updated by Ewan Birney, Dan Andrews and Graham McVicker)
-
-Revisions: EB Oct01, MC Jan02, MC Mar02, DA Jul02, new API revision DA Oct02, GMV Oct02, DA Feb03, MC
-Sep03
-
-_______________________________________________________________________
-
-Contents
-
-Introduction.
-Remote Access to EnsEMBL via MySQL.
-Introduction to the Perl object API.
- Companion script and exercises.
- What does the core EnsEMBL database contain?
- Set-up.
- Connection.
- Business objects and object adaptors.
- Let's get some data.
- Clones and RawContigs.
- Slices.
- Slices vs RawContigs.
- Sequence features.
- Analysis objects
- Overlaps
- Genes.
- Supporting evidence.
- Prediction transcripts.
- Translation.
- Protein.
- External databases - the general idea.
- SNPs.
- MarkerFeatures.
- A final, but important, note to users of older versions of the EnsEMBL API - Lists and List References
-
-_______________________________________________________________________
-Introduction
-
-It is important to us that you should be able to access the data in EnsEMBL databases as easily as possible. To try and
-meet the different needs of vastly differing data users we have developed a number of interfaces to the EnsEMBL data.
-You are probably reading this document because the web interface, while being very pretty and fast, is purely a web
-interface and won't provide the kind of bulk information you are needing. If you are a bioinformatician who is Perl aware,
-the API that this document describes is going to be very useful. Not only can you retrieve data directly through your
-scripts, to either remote or local EnsEMBL databases, you can do quite a lot of data processing via the tools built into the
-API data types. However, a point that needs to be made now is that you most probably DON'T have to use this API if your
-data needs are relatively simple. Instead, you can use the purpose-built data-mining tool called EnsEMBL Mart. We don't
-want to chase you away, but if you haven't already looked at Mart (www.ensembl.org/EnsMart/) we enthusiastically
-encourage you to do so soon.
-
-Okay. If you are still here, good. Now we can talk about accessing EnsEMBL databases, both directly and through the
-Perl API. The following document covers two broad areas. The first section pertains to accessing the public EnsEMBL
-MySQL server (kaka.sanger.ac.uk) and running queries with your own SQL. The second and larger section will introduce
-you to the EnsEMBL core API.
-
-In general, we've noticed that the best learning route for developers starting to use EnsEMBL is as follows:
-
-* Play around with the data on kaka.sanger.ac.uk with just a MySQL client.
-* Start using the object layer API (Perl based) against kaka.sanger.ac.uk (you will need to download EnsEMBL and
-bioPerl software).
-* Install the database locally. You may as well install the web site locally while you are about it, as it is pretty easy to get up
-and running.
-
-Of course, you might want to jump straight to installing the EnsEMBL web site, or the delve into the Perl API. Take your
-pick.
-
-Alternatively, you need no longer feel compelled to use Perl to work directly with EnsEMBL databases. If you want to
-work in Java you should definitely have a look at the rapidly growing Java API (www.ensembl.org/java/). This is not a
-subject covered in this document, so if you are interested please have a look at the Java specific documentation.
-
-Currently, a rather hard thing to do is to use the EnsEMBL software system to generate features de novo (i.e. run the
-gene building process) from raw sequence. This is called the "pipeline". To illustrate this, there are presently numerous
-remote EnsEMBL web sites but only a couple of remote pipelines. We expect that documentation and understanding of
-the pipeline will improve as more and more people attempt to run it remotely. The documentation of the pipeline that
-does exist is stored in our CVS repository, which is externally accessible. Perhaps the easiest way to obtain these
-documents in through our web-CVS (http://cvsweb.sanger.ac.uk/cgi-bin/cvsweb.cgi/ensembl-doc/). You are unlikely to
-get the pipeline working 100% properly without a bit of help, so if you are heading in this direction let us know and say
-hello.
-
-For other EnsEMBL documentation, have a browse through the EnsEMBL web site (www.ensembl.org) and follow the
-"Documentation" link. This should lead you to a number of useful "big" documents, including the one you are presently
-reading and the web-site installation instructions.
-
-For more detailed documents click on the "Wiki" link from the "Documentation" page. Some of the documents available
-in the Wiki are up to date, some (most) are not - caution. Once you are in the Wiki pages you can use the 'search' button to
-find what you are looking for. You can also browse the archives of the ensembl-dev mailing list for discussions which may
-have already answered some of your questions. If after doing this you still can't find what you need, please feel free to
-post a question on the ensembl-dev@ebi.ac.uk mailing list. We respond quickly and are nice people that don't bite, even
-if you haven't bothered to read the documentation (where it exists).
-
-Finally, if you find something in this document that is not clear or is incorrect, we will be most grateful if you let us know on
-the EnsEMBL developers mailing list (ensembl-dev@ebi.ac.uk). If you are shy, you can also email me directly
-(dta@sanger.ac.uk), I don't bite either.
-
-Remote Access to EnsEMBL via MySQL.
-
-EnsEMBL provides an internet accessible host (kaka.sanger.ac.uk) with the latest databases. This means you can do a lot
-of work from an internet connected host solely by using "client" software.
-
-You probably have a MySQL client installed if you are running a standard linux distribution. Try:
-
-mysql -u anonymous -h kaka.sanger.ac.uk
-
-If MySQL is not installed, go to www.mysql.com to download the client.
-
-Inside the MySQL prompt you generally want to start with the database "homo_sapiens_core_16_33" (at time of writing
-this was the most recent build) and the database "ensembl_mart_16_1". There are also databases for other species - you
-should be able to use these in the same way as described here for human.
-
-Try the "show databases" SQL command to see all the databases that are available, followed by a "use" command
-similar to "use homo_sapiens_core_16_33" to choose the current/most recent database. Other databases are
-named like "homo_sapiens_lite_16_1" - the naming scheme following the convention of species tag, underscore,
-database type, underscore and release number. Have a look around to see what you can use.
-
-Starting with the "core" database (homo_sapiens_core_16_33 or the most recent update of this database), try some of
-these nice queries to get a taste of what is stored in the databases:
-
-Choose the current human core database:
-
-"USE homo_sapiens_core_16_33;"
-
-Retrieve the first two DDBJ/EMBL/GenBank entries stored in the database:
-
-"SELECT clone_id FROM clone LIMIT 2;"
-
-Retrieve the first two confirmed genes in the database:
-
-"SELECT * FROM gene LIMIT 2;"
-
-Retrieve the DDBJ/EMBL/GenBank entries from the first 5Mb of human chromosome 1. The assembly information is
-stored in the assembly table, hence we need to perform a join across the clone, contig, chromosome and assembly
-tables:
-
- "SELECT DISTINCT(clone.name)
- FROM assembly, contig, clone, chromosome
- WHERE chromosome.name = '1'
- AND chromosome.chromosome_id = assembly.chromosome_id
- AND assembly.chr_end < 5000000
- AND assembly.contig_id = contig.contig_id
- AND clone.clone_id = contig.clone_id;"
-
-Each table has a unique, consistently-named identifier which can be used as foreign keys in other tables. The unique
-identifier column is always named tablename_id and contains integer values. For example, the unique identifier of
-the chromosome table is the contained in the chromosome_id column and the row with chromosome_id = 23
-corresponds to the chromosome named 'X'.
-
-Retrieve the exons which lie within the first 200,000 base-pairs of chromosome 1:
-
-"SELECT exon.exon_id
- FROM exon, assembly, chromosome
- WHERE chromosome.name = '1'
- AND chromosome.chromosome_id = assembly.chromosome_id
- AND assembly.chr_end < 500000
- AND assembly.contig_id = exon.contig_id;"
-
-(Note: Existing EnsEMBL users may notice that the exon.id column has been replaced by exon.exon_id which now
-contains an integer value. To get the ENSE number you will need to join to exon_stable. Be aware that the newest
-release of the EnsEMBL database represents quite a big shift in the schema compared to previous releases.)
-
-At this point you can see that constructing queries against the EnsEMBL core database in chromosomal coordinates will
-always mean joining to the assembly table. This is a bit painful. Thankfully we have developed a query-optimised database
-that is derived from this database (a datamart in trendy computer speak), called EnsEMBL Mart. This is changing rapidly,
-but is well worth playing around with:
-
-"USE ensembl_mart_16_1;"
-
-To genes from a particular region:
-
-"SELECT gene_stable_id FROM homo_sapiens_core_gene WHERE chr_name = '1' AND
-gene_chrom_end < 200000;"
-
-Or from a particular band:
-
-"SELECT gene_stable_id FROM homo_sapiens_core_gene WHERE band LIKE 'q25.%' AND chr_name =
-'1';"
-
-(notice the use of LIKE to truncate to the major band)
-
-EnsEMBL Mart will expand over time to allow progressively richer queries. To investigate more use the commands:
-
-"SHOW tables;"
-
-and
-
-"DESCRIBE tablename ;"
-
-So, now you can play with the data, but where are things like the translations or cDNAs? Additionally, how can I find all the
-BLAST hits which overlap exons that live in my genomic region of interest? To do this you will need to write code often.
-Do yourself a favour and take advantage of our API which we use every day to wrangle this kind of data.
-
-Introduction to the Perl object API
-
-This document grew from notes prepared for an introductory half-day course that we occasionally run on the EnsEMBL
-code base (0.5M lines of code in half a day - now there's value). They will take you through the process of connecting to
-an EnsEMBL database and accessing the data contained therein. The information contained here is by no means
-exhaustive, but should give you enough knowledge to be able to competently use the core EnsEMBL code. After
-learning how to retrieve genes, exons, sequence and process these to your own ends, we hope you will feel confident
-enough to delve deeper into other less-trodden parts of the code that may interest you.
-
-Before you start, you will need to have the relevant EnsEMBL and BioPerl modules installed. These are :
-
-bioperl-0.7 (we know this is not the latest version - for now, EnsEMBL is tied to it, so please use it.)
-ensembl
-ensembl-external
-
-Instructions on how to install these perl modules are contained on the EnsEMBL website www.ensembl.org. Basically,
-you need to do the following steps (in both cases below we are using cvs to get the code, which is much better than ftp as
-we are getting the latest bug fixes. Notice the - r flag to the cvs commands. These indicate the branch of each repository
-to get out. (Branches are stable versions of the code).
-
- BioPerl (see cvs.bioperl.org for more details)
-cvs -d :pserver:cvs@cvs.bioperl.org:/home/repository/bioperl login
-when prompted, the password is 'cvs'
-cvs -d :pserver:cvs@cvs.bioperl.org:/home/repository/bioperl checkout -r branch-07
-bioperl-live
-
- EnsEMBL
-
-cvs -d :pserver:cvsuser@cvsro.sanger.ac.uk:/cvsroot/CVSmaster login
-when prompted, the password is CVSUSER
-cvs -d :pserver:cvsuser@cvsro.sanger.ac.uk:/cvsroot/CVSmaster checkout -r branch-ensembl-
-16 ensembl
-cvs -d :pserver:cvsuser@cvsro.sanger.ac.uk:/cvsroot/CVSmaster checkout -r branch-ensembl-
-16 ensembl-external
-
-If you don't have, or don't want to install, the EnsEMBL database locally (which is all you will need to complete the tutorial
-exercises) you can point your scripts at a publically available one at the Sanger Centre. Use the following fields in your
-scripts (where X_X is the latest version of the database):
-
-host kaka.sanger.ac.uk
-dbname homo_sapiens_core_X_X
-user anonymous
-
- DBI and DBI::mysql
-
-Unless you already have them installed, before you can begin you will need to install the Perl DBI and DBI::mysql modules
-from the CPAN (www.cpan.org). See the CPAN site for instructions on how to do this.
-
-
-Companion script and exercises
-Accompanying this document is a script called tutorial.pl. This script contains all the example code in this document
-and should run successfully if you have the right database and version of the code installed. In addition, soon there will
-be worked solutions to the exercises included later in this tutorial. When you check-out a copy of EnsEMBL from the
-cvs, both the companion script and the pending worked solutions will be in the ensembl/docs/tutorial directory.
-
-What does the core EnsEMBL database contain?
-
-*Clones (with embl accessions) - both finished and unfinished.
-
-*Each contiguous piece of sequence is called a contig. These are the basic lengths of DNA that we analyse and
-annotate.
-
-*Each clone will contain one or more contigs.
-
-*Finished clones = one contig.
-
-*Unfinished clones = any number of contigs.
-
-*Each contig (whether finished or unfinished) has certain features associated with it that represent the result any one of
-the various analysis programs that have been run on it (e.g. RepeatMasker, BLAST, genscan). These are the basic
-computes used to build the genes.
-
-*The raw analysis results are used to build genes which are also stored in the database. Each gene contains one or more
-transcripts and each transcript will contain a translation.
-
-*Each gene has various information attached to it describing whether it is a known gene or corresponds to a SwissProt or
-trEMBL protein. Some genes are novel genes which have been built by inference from similarities to other sequences.
-These novel genes won't have a corresponding SwissProt or trEMBL protein.
-
-*Each translation has had a variety of protein analyses conducted on it and you can access information about the results of
-these. This includes information derived from pfam, prosite and prints.
-
-*There are other features accessible through non-core EnsEMBL external databases. Such databases may contain
-information about SNPs, mouse trace hits or embl annotations. You can create your own external databases to be
-incorporated into EnsEMBL, but this is a subject for a more advanced document.
-
-Setup
-
-Before starting with the EnsEMBL modules you will need to set up your environment so Perl knows where to find them.
-As EnsEMBL is built on top of BioPerl (version 0.7 for now), this includes telling it where bioperl-0.7 lives on your system.
-
-The environment variable to do this is PERL5LIB. If you are using csh or tcsh you need to type in the following (changing
-/nfs/croc/michele/branch to your directory containing the perl modules you downloaded). If you are concomitantly using a
-version of BioPerl newer than version 0.7 make sure that the older version appears in your Perl library path before the
-newer version.
-
-setenv ENSHOME /nfs/croc/michele/branch
-
-setenv PERL5LIB $ENSHOME/ensembl/modules:$ENSHOME/bioperl-0.7:$ENSHOME/ensembl-
-external/modules:$ENSHOME/ensembl-lite/modules
-
-It has become apparent recently that the EnsEMBL code-base just won't run on older versions of Perl. We use version
-5.6 for development and testing. We recommend that you should do so too. If you are unsure of what version is running
-on your machine. Type:
-
-perl -v
-
-Presuming that this is all in order, you are now ready to write your first script.
-
-Connection
-
-All data used and created by EnsEMBL is stored in a MySQL relational database. If you want to access this database the
-first thing you have to do is to connect to it. This is done behind the scenes by EnsEMBL using the famous Perl module
-called DBI. You will need to know three things before you start :
-
- host the hostname where the EnsEMBL database lives
- dbname the name of the EnsEMBL database
- user the username to access the database
-
-First, we need to declare to Perl the modules we want to use so it can go and check the syntax of them. This is done by a
-use statement. This line has to be inserted into all your EnsEMBL scripts:
-
-use Bio::EnsEMBL::DBSQL::DBAdaptor;
-
-The we set the all important variables telling Perl where and what your database is:
-
-my $host = 'kaka.sanger.ac.uk';
-my $user = 'anonymous';
-my $dbname = 'homo_sapiens_core_16_33';
-
-Now we can make a database connection:
-
-my $db = new Bio::EnsEMBL::DBSQL::DBAdaptor(-host => $host,
- -user => $user,
- -dbname => $dbname);
-
-We've made a connection to an EnsEMBL database and passed parameters in using the -attribute =>
-'somevalue' syntax present in many of the EnsEMBL object constructors. Formatted correctly, this syntax lets you see
-exactly what arguments and values you are passing.
-
-The $db variable now contains a reference to the newly created DBAdaptor object which you can start using to extract
-data. If, heaven forbid, the connection fails an error message will be returned.
-
-Business Objects and Object Adaptors
-
-Before we launch into the ways the API can be used to retrieve and process data from the EnsEMBL databases it is best
-to mention the fundamental relationships the EnsEMBL objects have with the database.
-
-The EnsEMBL API allows manipulation of the database data through various business objects. For example, some of the
-more heavily used objects are the Gene, Clone and Exon objects. More details of how to effectively use these objects will
-be covered later. These objects are retrieved and stored in the database through the use of object 'adaptors'. The object
-adaptors have internal knowledge of the underlying database schema and use this knowledge to fetch, store and remove
-objects (and data) from the database. This way you can write code and use the EnsEMBL API without having to know
-anything about the underlying databases you are using. The database adaptor (that we obtained by connecting to our
-database, in the previous section) is a special adaptor which has the responsibility of maintaining the database connection
-and creating other object adaptors.
-
-As will be shown later in this document, to obtain object adaptors from the main database adaptor there is a suite of
-methods that have the naming convention "get_ObjectAdaptor". To obtain a SliceAdaptor or a CloneAdaptor
-(which, oddly enough, retrieve Slice and Clone objects) do the following:
-
-my $gene_adaptor = $db->get_GeneAdaptor();
-my $clone_adaptor = $db->get_CloneAdaptor();
-Don't worry if you don't immediately see how useful this could be. Just remember that you don't need to know anything
-about how the database is structured, but you can retrieve the necessary data (neatly packaged in objects) by asking for it
-from the correct adaptor. Throughout the rest of this document we are going to work through the ways the EnsEMBL
-business objects can be used to derive the information you want.
-
-Let's get some data
-
-Once you have a database adaptor, you are ready to retrieve data. Conceptually, there are two main ways to go about
-retrieving and manipulating genome data. Historically, data has been handled on a clone- by- clone basis. The EnsEMBL
-API is fully capable of working with the clone- based data that comes out of a sequencing project, and fundamentally
-EnsEMBL works with data at a contig level. However, due to the nearing completion of the first big genome sequencing
-projects it is now becoming possible to practically work with genomes as long stretches of gap-less sequence. EnsEMBL
-also allows you to work with sequence information in this way. Previous releases of the EnsEMBL API called these un-
-interrupted stretches of sequence virtual contigs. From release 9 onwards these have been called Slices - literally, slices
-of genomic sequence. All you need to do to retrieve such a sequence is stipulate the chromsome number and the start
-and end coordinates - we'll get to doing this in just a bit.
-
-Most users of the EnsEMBL API will probably end up using both means of retrieving and manipulating genome
-sequences. Hence, we'll introduce both of these here in the next two sections.
-
-
-Clones and RawContigs
-
-Clones and RawContigs represent two basic data-types in EnsEMBL. Clones have contigs, and contigs are the elements
-of contiguous sequence information that are built up to create the full clone sequence. Once a clone sequence is
-complete, the clone will be represented by one contig sequence.
-
-If you know the accession number of a clone that you are interested in, you can retrieve that clone directly from the
-CloneAdaptor in the following way:
-
-my $clone = $clone_adaptor->fetch_by_accession('AC005663');
-
-This will return a Clone object. You can check whether you have the right clone by calling the Clone object's id method:
-
-print "Clone is " . $clone->id . "\n";
-
-To get the most use out of this object we now need to ask it about its contigs (remember - clones have one or more
-contigs).
-
-my $contigs = $clone->get_all_Contigs;
-
-We now have a reference to a list of RawContig objects. RawContig objects are actually bioperl sequence objects and this
-means they can be used to retrieve all sorts of useful information,
-
-Say we want to get the sequence of each contig:
-
-foreach my $contig (@$contigs) {
- my $length = $contig->length;
- my $id = $contig->id;
-
- # The sequence of a contig is obtained easily:
-
- print $contig->seq . "\n";
-
- # We can get a substring of this sequence too:
-
- print $contig->subseq(1,100) . "\n";
-
- # If we want to write the sequence to a file we use bioperl again
- # (Note: if we are creating a new object we need to include
- # a use Bio::SeqIO line at the start of our file
-
- use Bio::SeqIO;
-
- my $seqio = new Bio::SeqIO(-fh => \*STDOUT,
- -format => 'fasta');
-
- $seqio->write_seq($contig);
-
-}
-
-Many features can be obtained from a RawContig. One set of features that will prove extremely useful if you're going to
-do any analysis on them are the repeat features. These can be used to mask the sequence ready for, say, a BLAST
-search. For example:
-
-my $maskedseq = $contig->get_repeatmasked_seq;
-
-Hence, EnsEMBL provides you with a handy call to obtain repeat-masked sequence without you having to rerun
-RepeatMasker.
-
-Of course, given a whole genome sequence to play with, you are hardly going to be content with looking at just one
-clone. Hence, to do genome sized analyses you will probably want to retrieve all the clones from the database. Before
-going further it is best to point out that unless you have an over-riding reason to work on a clone-by-clone basis, this kind
-of analysis might be more easily done using Slices, which are the subject of the next section. However, if you need
-clones, you can retrieve them in the following way.
-
-my $clones = $clone_adaptor->fetch_all;
-
-Once this list of clones has been obtained you can set about getting useful things.
-
-foreach my $clone (@$clones) {
- my $contigs = $clone->get_all_Contigs;
- foreach my $contig (@$contigs) {
- my $sequence = $contig->seq();
- print $sequence . "\n";
- }
-}
-
-Notice that the above script allows you to print the entire genome sequence, which is potentially quite slow. Unless you
-have a reason to laboriously work through clone and contig sequences, you will be much better off using Slices - which
-we will come to just around the next corner.
-
-Exercises 1
-
-1. Connect to the database. How many clones does it contain?
-
- (Hint: Make a $db object and get a clone adaptor from it. Use the fetch_all() method of the adaptor and then take the
-size of returned array).
-
-2. What is the average number of raw contigs per clone for the first 100 clones in the database?
-
- (Hint: get_all_Contigs on clone);
-
-3. Print out in fasta format the repeat-masked sequence for the last 10 clones of the genome.
-
-
-Slices
-
-If you have had experience with using the EnsEMBL API before, you will have come across the concept of virtual contigs.
-A virtual contig was an EnsEMBL object that represented a section of a genome. These virtual contigs could be created
-to represent any single part of the genome, and were created simply by making a call to the $db object specifying start and
-end coordinates for a particular chromosome. The nitty gritty of taking the actual raw contig sequences and stitching
-these together into a single sequence was done in the background by the EnsEMBL code. The current incarntion of a
-virtual contig is the Slice - virtual contigs are no longer part of the EnsEMBL code. Just like virtual contigs a Slice is an
-EnsEMBL object that represents any particular region of a genome. A Slice and its associated methods provide a simple
-way to retrieve and manipulate sequence from a part of the genome that you are interested in. To get a Slice of genome
-you just need to do the following.
-
-First, get a Slice adaptor from the database adaptor:
-
-my $slice_adaptor = $db->get_SliceAdaptor;
-
-With a Slice adaptor, retrieving genome sequence is as easy as specifying which region of which chromosome we are
-after:
-
-my $slice = $slice_adaptor->fetch_by_chr_start_end('1',1,100000);
-
-The order that the arguments are passed is chromosome number, start coordinate and end coordinate. Notice that this is
-reflected in the naming of the slice adaptor method. Generally this is a good guide to guessing what arguments you need
-to provide a method call - alternatively you can have a look at the documentation associated with a particular module.
-
-Another useful way to obtain a Slice is to do it with respect to a gene it contains:
-
-my $slice = $slice_adaptor->fetch_by_gene_stable_id('ENSG00000099889', 5000);
-
-This will return a Slice that contains the sequence of the gene specified by its stable EnsEMBL id. It also returns 5000bp
-of flanking sequence at both the 5' and 3' ends, which is obviously very useful if you are interested in the environs that a
-gene inhabits. You needn't have the flanking sequence it you don't want it - in this case set the number of flanking bases
-to 0 or omit the second argument entirely.
-
-We can also retrieve Slices of actual physical contigs - and we can pad these at the ends with sequence from adjoining
-contigs:
-
-my $slice = $slice_adaptor->fetch_by_contig_name('AC005663.2.1.103122', 10000);
-
-This returns a Slice that contains the contig that was specified by name. It also includes 10000bp of flanking sequence at
-each end of this actual contig.
-
-We can also do the same with clones:
-
-my $slice = $slice_adaptor->fetch_by_clone_accession('AC005663',1000);
-
-So, once we have our Slice we can then use it to retrieve useful information about the region of sequence that the Slice
-represents.
-
-Just like RawContigs mentioned previously, a Slice is a BioPerl sequence object and we we can get at the actual
-sequence information like thus:
-
-my $sequence = $slice->seq;
-
-We can query the Slice for information about itself:
-
-my $chr_name = $slice->chr_name;
-my $chr_start = $slice->chr_start;
-my $chr_end = $slice->chr_end;
-
-print "Chromosome $chr_name Start/End $chr_start $chr_end \n";
-
-The makeup of our Slice is determined by the assembly (or the golden tiling path) of our genome. If we wanted to access
-this assembly information with regard to a particular Slice we can do this by:
-
-my $tiling_path = $slice->get_tiling_path();
-
-foreach my $tile (@$tiling_path) {
- my $tile_slice = $tile->assembled_Seq()->name;
- my $tile_ctg = $tile->component_Seq()->name;
-
- my $slice_start = $tile->assembled_start();
- my $slice_end = $tile->assembled_end();
- my $ctg_start = $tile->component_start();
- my $ctg_end = $tile->component_end();
- my $ctg_ori = $tile->component_ori();
-
- print "[$tile_slice] $slice_start - $slice_end => " .
- "[$tile_ctg] $ctg_start - $ctg_end ($ctg_ori)\n";
-}
-
-Each 'tile' that we are returned is a Bio::EnsEMBL::Tile object representing the portion of a slice that maps directly onto a
-portion of the assmebly (or golden path).
-
-A Slice is a good starting place for retrieving information about a region of a chromosome, including the genes it contains.
-The next few sections will go into more detail about how to do this.
-
-
-Slices vs RawContigs
-
-Before going on to the next topics an important point needs to be made. Despite the fact that Slices and RawContigs are
-different objects and don't represent exactly the same thing (one is an experimentally generated stretch of sequence, the
-other is a computationally pooled sequence) they can effectively be used as such. In the rest of this document a
-distinction won't be drawn between the two - unless there is an actual difference between how the two objects behave
-(which by design should be few). We will be using Slices by default though, as we would encourage you to do too.
-Exercises 2
-1. Fetch 1Mb of repeat-masked sequence from the contig of your choice (Hint: Create a Slice using
-fetch_Slice_by_chr_start_end($chr,$start,$end) and have a look back at how this was done with
-RawContigs in the previous section).
-2. Fetch a Slice of the gene ENSG00000100259
-3. Print the last 1000 nucleotides of the Silce obtained in the previous question.
-3. For the Slice you retrieved in the first question, print the ids of the contigs that form the tiling path. Also print the start
-and end positions of each RawContig as it is used in the tiling path.
-
-Sequence Features
-
-So now we're pretty happy about retrieving DNA sequence from EnsEMBL. The more interesting things associated with
-Slices and RawContigs are the features that can be retrieved from them. These include the repeat features mentioned in
-previously and also similarity features (like BLAST results), prediction transcripts (such as genscan results) and marker
-features. Not to mention genes, of course.
-
-Sets of features can be retrieved from a region through a request to a Slice or RawContig (you're probably getting used to
-this by now). These are returned to us as feature *objects*. For instance:
-
-my $repeats = $slice->get_all_RepeatFeatures;
-
-We now have a reference to a list containing feature objects. An easy way to print these out is to call the method
-gffstring which returns information about the feature as a string:
-
-foreach my $repeat (@$repeats) {
- print $repeat->gffstring . "\n";
-}
-
-You should get a series of output lines like
-
-22:16890204-16987127 RepeatMasker repeat 2851 2959 645 + -1
-22:16890204-16987127 RepeatMasker repeat 2979 3260 2066 - -1
-22:16890204-16987127 RepeatMasker repeat 4008 4056 234 - -1
-22:16890204-16987127 RepeatMasker repeat 4080 4345 1768 - -1
-22:16890204-16987127 RepeatMasker repeat 4364 4450 549 - -1
-22:16890204-16987127 RepeatMasker repeat 4650 4736 252 - -1
-22:16890204-16987127 RepeatMasker repeat 5835 5972 413 - -1
-22:16890204-16987127 RepeatMasker repeat 12035 12084 193 - -1
-
-The columns that compose a gffstring have the following meaning:
-
-1 sequence name
-2 feature type
-3 main feature type
-4 sequence start
-5 sequence end
-6 strand
-7 score
-8 phase
-
-Sometimes there are an additional three columns if the feature type posesses them:
-
-9 hit sequence name
-10 hit start
-11 hit end
-
-
-Instead of using gffstring we can ask the feature objects directly about their properties:
-
-foreach my $repeat (@$repeats) {
- print "Name : " . $repeat->seqname . "\n";
- print "Start : " . $repeat->start . "\n";
- print "End : " . $repeat->end . "\n";
- print "Strand : " . $repeat->strand . "\n";
- print "Score : " . $repeat->score . "\n";
-
-}
-
-Some features (like CpG islands for instance) are simple features and only have the methods printed above.
-
-Other features as well as having coordinates on a contig sequence also have coordinates on a hit sequence:
-
-foreach my $repeat (@$repeats)
- print "Hit start " . $repeat->hstart . "\n";
- print "Hit end " . $repeat->hend . "\n";
-}
-
-Repeat features are an example of a feature which also stores the coordinates of a hit sequence. However, the classic
-example is a feature representing a BLAST result where the query sequence is similar to another sequence (protein,
-EST, cDNA) and so we need to store which sequence it has hit and whereabout in that sequence it has hit. These types
-of features have the generic name of similarity features. In reality these features are members of a superclass called
-AlignFeatures (because they are features with two aligned sequences), and depending on whether it is DNA or protein
-sequence that we are dealing with, the objects are either a Bio::EnsEMBL::DnaDnaAlignFeature or a
-Bio::EnsEMBL::ProteinDnaAlignFeature
-
-To get similarity features from a RawContig or a slice we use:
-
-my $features = $contig->get_all_SimilarityFeatures;
-
-To obtain only DnaAlignFeatures we can use the following method:
-
-$features = $slice->get_all_DnaAlignFeatures('Vertrna', 0.00001);
-
-Note that this method takes two optional arguments which can impose an additional constraint on the features we want to
-retreive. The first argument is a 'logic name' - the name for the analysis which was conducted to obtain the feature. For
-similarity features the logic name relates to the type of blast analysis and there are currently four logic_names which
-correspond to dna_align_features in the current code (note that the logic name is CASE SENSITIVE):
-
-Vertrna WU tblastn hits against the EMBL-vertrna database (all CDS's from the EMBL database).
-Swall WU blastp hits against the SWALL database (protein features only).
-Unigene WU tblastn hits against the UniGene database.
-dbEST WU tblastn hits against the dbEST database.
-
-Hence, in our call to retrieve DNA features, we have requested those that are hits against the EMBL vertrna database. We
-have also stipulated a cut- off value. This allows us to set the level of strigency of BLAST hits and is exactly the same as
-the BLAST E(0) value.
-
-Once we have these DNA features we can iterate through them and take a look at what they contain:
-
-foreach my $feat (@$features) {
- print "Feature scores are " . $feat->score . "\t" .
- $feat->percent_id . "\t" .
- $feat->p_value . "\n";
-}
-
-The same procedure applies to retrieving protein features, just instead use the method call:
-
-$slice->get_all_ProteinAlignFeatures('Swall', 0.0001).
-
-Choose a logic name appropriate to proteins - so stick with 'Swall' (or leave the logic name out entirely). Once these
-protein features have been returned we can look at them in the same way as we just did for DNA features.
-
-As an alternative approach we can go directly to the object adaptor for a feature type and request features from the
-database. The following example illustrates some of the different approaches that can be used to obtain features:
-
-my $simple_feature_adaptor = $db->get_SimpleFeatureAdaptor;
-
-#get all of the Eponine hits on a Contig
-my @eponine_hits = @{$simple_feature_adaptor->fetch_all_by_RawContig($contig,
-'Eponine')};
-
-#get all CpG islands on a Slice with score greater than 500
-my $cpg_islands = $simple_feature_adaptor->fetch_all_by_Slice_and_score($slice, 500,
-'CpG');
-
-#get the simple feature with the database identifier 1
-my $simple_feature = $simple_feature_adaptor->fetch_by_dbID(1);
-
-#get all of the simple features on a slice
-my @simple_features = @{$slice->get_all_SimpleFeatures};
-
-#get all of the tRNAscan hits on a contig
-my $trnas = $contig->get_all_SimpleFeatures('tRNAscan');
-
-Analysis Objects
-
-All features retrieved from the database have an Analysis object attached to them that contains information regarding the
-analysis/algorithm that created the feature. This is a good means by which to determine where the feature has come from
-or what it actually is. The gff_source method of the Analysis object tells us what sort of feature we have, such as
-'genscan','repeat' or 'cpg'. Other information can be derived from the Analysis object, such as the database that was used
-in a BLAST job, the parameters that were provided to the executable, and even the path to the executable that was used:
-
-my $analysis = $feature->analysis;
-
-print "Analysis: " . $analysis->gff_source . "\n";
-print "Database : " . $analysis->db . "\n";
-print "Program : " . $analysis->program . "\n";
-print "Parameters : " . $analysis->parameters . "\n";
-
-For instance a feature that comes out of a BLAST run will have an analysis object that tells us it was run with 'blastx' and
-was run against database 'sptr';
-
-Overlaps
-
-A useful feature of BioPerl is that it makes it easy to find whether one feature overlaps another. This comes in jolly handy if
-you want to find all genscan predictions that don't overlap exons, or all SNPs that do overlap exons, or mouse trace hits
-that don't overlap exons, etc.
-
-If we have two features - say an exon, $exon, and a snp, $snp:
-
- if ($exon->overlaps($snp)) {
- print "Whey! coding snp " . $snp->gffstring . "\n";
- } else {
- print "Boo! non coding snp " . $snp->gffstring . "\n";
- }
-
-The overlaps method returns true (1) if the two features do overlap, or false (0) if they don't.
-
-Exercises 3
-
-1. Print out all the repeat features for the first 100kb of sequence of human chromsome 1. (Hint: Use the gffstring
-method for easy printing)
-
-2. What proportion of DNA is repeat in the above sequence and is this what you expect? (Hint: tot up the length for each
-repeat feature and compare to the total sequence length)
-
-3. For clone AC005663 retrieve all the DNA features matched to EMBL vertrna. How many different sequences did this
-clone hit and were these hits significant? (Hint: Use the hseqname method and the p_value method)
-
-Genes
-
-During the pipeline gene building process, genes are built on Slices because a lot of genes span more than one contig.
-Hence , it makes the most sense to subsequently use Slices to access them. However, having said that you can access
-genes via RawContigs if you wish. Getting all the of the genes from a Slice or RawContig is as easy as:
-
-my $genes = $slice->get_all_Genes;
-
-As usual we are returned a reference to a list of objects - Gene objects this time. They contain all the information about the
-exon/intron structure, DNA sequence, etc.
-
-Genes have EnsEMBL identifiers which can be accessed using the stable_id method:
-
-foreach my $gene (@$genes) {
- print "Gene : " . $gene->stable_id . "\n";
-}
-
-EnsEMBL identifiers don't really tell us much about the gene (and they're not intended to) and some genes will have one
-or more more common names.
-
-We can tell immediately if a gene is a known gene (refseq or sptrembl) by calling the is_known method. If it is a known
-gene then we can call the each_DBLink method to find out more about it.
-
-foreach my $gene (@$genes) {
- if ($gene->is_known) {
- foreach my $link (@{$gene->get_all_DBLinks}) {
- print "Gene " . $gene->stable_id . " links to " .
- $link->display_id . " " .
- $link->database . "\n";
- my @syns = @{$link->get_all_synonyms};
- print "Synonyms for gene are @syns\n";
- }
- } else {
- print "Gene " . $gene->stable_id . " is not a known gene\n";
- }
-}
-
-Other information about a known gene is contained in its description method. This information is extracted from the
-relevant swissprot or refseq entry.
-
-my $description = $gene->description;
-
-Genes are quite complicated objects and are constructed thusly.
-
-Each Gene object has one or more Transcript objects (one for each alternatively spliced cDNA).
-
-my $transcripts = $gene->get_all_Transcripts();
-
-Each Transcript is made up of a series of Exons. We can access the Exons and retrieve their sequence and coordinates.
-
-foreach my $exon (@{$transcript->get_all_Exons}) {
- print "Found exon " . $exon->stable_id . " " .
- $exon->start . " " .
- $exon->end . " " .
- $exon->seq->seq;
-}
-
-Notice that calling the seq method on an Exon object returns us a BioPerl sequence object. We then have to call seq
-again to get a string representation of the sequence. Hence, we get the seq->seq stutter - its not a typo in case you
-were wondering.
-
-We can get the protein sequence of a Transcript by calling the translate method. So to get all the protein translations
-from a gene into a file we would do
-
-my $seqio = new Bio::SeqIO->(-format => 'fasta',
- -fh => \*STDOUT);
-
-foreach my $transcript (@{$gene->get_all_Transcripts}) {
- my $peptide = $transcript->translate;
-
- $seqio->write_seq($peptide);
-}
-
-Note that when writing to a BioPerl SeqIO object we pass the Peptide object and not a string, but when writing out to the
-screen we have to stringify it first.
-
-Exercises 4
-1. Get all the genes on a long- ish (say 1Mb) Slice and print their EnsEMBL ids.
-
-2. Which of those genes are known genes and what is their more common name? (Hint. Use the is_known method and
-use the get_all_DBLinks->display_id)
-
-3. How many genes are alternatively spliced for the first 100 genes (Hint: Count the number of transcripts using the
-$gene->each_Transcript method)
-
-4. What is the average size and number of exons per gene (Hint: Use the get_all_Exons method and remember that
-exons are like features with $ex->start $ex->end)
-
-5. Translate the first 10 genes - are there any stop codons (there shouldn't be!!) (Remember there may be more than one
-transcript per gene)
-
-6. Print out the 200 bases of sequence that flanks each exon (Use the $gene->get_all_Exons method and call $contig-
->subseq($start,$end) method to retrieve the dna)
-
-7. Extract all the introns from the first 10 genes and write them to a file (be careful about reverse strand genes).
-
-8. Print out all the 5' and 3' utrs for the first 10 genes.
-Supporting Evidence
-
-The information that was used to make a gene is also stored in the EnsEMBL database in the form of FeaturePairs. This
-information can be retrieved easily from a Gene object.
-
-The evidence is attached as an array of FeaturePairs to each Exon and can be retrieved in the following way:
-
-foreach my $gene (@$genes) {
- my $transcripts = $gene->get_all_Transcripts;
- foreach my $transcript (@$transcripts) {
- my $exons = $transcript->get_all_Exons;
- foreach my $exon (@$exons) {
- my $supporting_features = $exon->get_all_supporting_features;
- foreach my $feature (@$supporting_features) {
- print "Evidence " . $feature->gffstring . "\n";
- }
- }
- }
-}
-
-Prediction Transcripts
-
-EnsEMBL stores ab initio gene predictions from genscan. These predictions are accesses in a similar fashion to normal
-EnsEMBL transcripts , but through PredictionTranscript objects rather than Transcript objects. As with normal transcripts
-prediction transcripts are composed of a set of Exon objects. To obtain prediction transcripts from a Slice or RawContig
-we can do the following (as with other EnsEMBL features logic name or score constraints can be passed as optional
-arguments) :
-
-my $predicted_transcripts = $slice->get_all_PredictionTranscripts;
-
-We now have a reference to a list of PredictionTranscript objects. To have a look at the predicted exons for each
-predicted transcript we can use the following:
-
-foreach my $predicted_transcript (@$predicted_transcripts) {
- my $exons = $predicted_transcript->get_all_Exons;
-
- print "Genscan prediction has " . scalar @$exons . : exons\n";
-
- foreach my $exon (@$exons) {
- print $exon->start . " - " .
- $exon->end . " : " .
- $exon->strand . " " .
- $exon->phase ."\n";
- }
-}
-
-As these predictions should translate there you can get the predicted peptide sequence by using the translate
-method on a genscan prediction.
-
-print "Genscan peptide is " . $predicted_transcript->translate->seq . "\n";
-
-Translation
-
-Each Transcript, retrieved from a Gene object , can be split into a 5' untranslated region, a CDS and a 3' untranslated
-region. The points in the cDNA where the translation starts and stops are stored in a Translation object which is attached
-to each Transcript:
-
-my $translation = $transcript->translation;
-
-The Translation object has methods:
-
-$translation->start_Exon;
-$translation->end_Exon;
-
-which denote which exons the translation starts and ends in. To find the exact coordinate of the start and stop of
-translation use the methods:
-
-$translation->start;
-$translation->end;
-
-The start and end methods refer to the exon coordinates so they should never be less than one or greater than the
-exon's length.
-
-Protein
-
-Information regarding a protein can be retreived from the ProteinAdaptor via a Translation object identifier:
-
-my $protein_adaptor = $db->get_ProteinAdaptor;
-
-my $protein = $protein_adaptor->fetch_by_translation_id($translation->dbID);
-
-As every transcript has only one translation we can fetch proteins using the transcript identifier as well:
-
-my $protein = $protein_adaptor->fetch_by_transcript_id($transcript->dbID)
-
-Once we have a Protein object we can look at its features
-
-my $protein_features = $protein->get_all_ProteinFeatures;
-
-The features that are returned are ProteinFeatures. To have a look at them you can use the gff_string method:
-
-foreach my $pf (@$protein_features) {
- print $pf->gffstring . "\n";
-}
-exercises 5
-
-1. Find all the pfam domains contained in the first 100 genes on chromosome 1. Which ones are most common and is
-this surprising?
-
-2. How many of those genes have no protein features at all.
-
-3. How many of the proteins contain WD40 domains?
-
-External Databases - The General Idea
-
-The core EnsEMBL database (the one you've been using up to now) contains DNA, genes and some sequence
-features. There are extra satellite databases that contain other features that can also be accessed.
-
-The idea is that you take your main EnsEMBL database handle ($db - way back in the first section) and give it another
-database handle to look after. This external database could contain all manner of things e.g. SNPs, mouse trace hits or
-EMBL annotations. You can now access the features in the second database as though they were all in the same place
-even though they could be sitting on a completely different machine.
-
-Let's give the EST database as a first example.
-
-First we need to connect to the main EnsEMBL database:
-
-use Bio::EnsEMBL::ExternalData::ESTSQL::DBAdaptor;
-
-my $est_db = Bio::EnsEMBL::ExternalData::ESTSQL::DBAdaptor->new(
- -host => $host,
- -dbname => $estname,
- -user => $user);
-
-And then to the human EST database to our main db adaptor and vice-versa:
-
-$db->add_db_adaptor('est', $est_db);
-$est_db->add_db_adaptor('core', $db);
-
-Now that we have done this we can retrieve the EST alignment features from this the database in the same way as we
-would other DNA align features by specifying the name of the analysis that was used to generate the alignments - in this
-case 'ex_e2g_feat'.
-
-my $est_features = $slice->get_all_DnaAlignFeatures('ex_e2g_feat');
-
-Now that we have these features we can have a look at them:
-
-foreach my $est (@$est_features) {
- print " " . $est->gffstring . "\n";
-}
-
-SNPS
-
-SNP features can be readily accessed from the external database ensembl-lite:
-
-use Bio::EnsEMBL::Lite::DBAdaptor;
-
-my $lite_db = Bio::EnsEMBL::Lite::DBAdaptor->new(-host => $host,
- -user => $user,
- -dbname => $litename);
-
-Add the lite db adaptor to the main db adaptor and vice-versa like before:
-
-$db->add_db_adaptor('lite', $lite_db);
-$lite_db->add_db_adaptor('core', $db);
-
-Retrieve all the SNP features from a Slice using the get_all_SNPs method:
-
-my $snp_features = $slice->get_all_SNPs;
-
-Iterate through the SNP features printing the position at which they lie along the Slice:
-
-foreach my $snp (@$snp_features) {
- print "snp " . $snp->start . "\n";
-}
-
-
-Marker Features
-
-The lite database also contains information about markers. Given that we already have a warm ensembl-lite database
-adaptor, it is too much to resist retrieving these too.
-With one call we can retrieve all the marker features on our Slice:
-
-my @markers = @{$slice->get_all_MarkerFeatures};
-
-Exercises 6
-
-1. Connect to the core EnsEMBL database and the SNP database. Add the SNP database to the EnsEMBL database
-and vice-versa.
-
-2. Create a Slice of 1Mb of sequence from your chromosome of choice (hint - remember back to the Slice exercises)
-
-3. How many SNPs are there in this region?
-
-4. How many SNPs overlap exons? (Hint - get all genes from the Slice and use get_all_Exons to get the exons from
-the genes. Use the overlaps method to calculate the proportion of SNPs that are situated in coding sequences.)
-
-
-A final, but important, note to users of older versions of the EnsEMBL API - Lists and List
-References
-
-The EnsEMBL API has just recently passed through a rapid period of change. A significant difference between old and
-new versions of the API is the now widespread use of list references (listrefs). Some method calls can potentially return
-very long lists and it is far more efficient, both time- and memory-wise, for these methods to return references to these lists
-instead. Although not all methods return large arrays of objects, for consistency almost all methods in the API should now
-return list references rather than lists/arrays. A naming standard has also been developed which should make it easier for
-API users to surmise which methods return a single value or return a list reference. Business object methods with the
-prefix get_all_ return a list reference, as do adaptor methods with the prefix fetch_all_ . If you are returned a
-listref from a method call it is a simple matter to de-reference this. The following examples illustrate the use of list
-references in the EnsEMBL API:
-
-#fetch all clones from the clone adaptor
-my $clones = $clone_adaptor->fetch_all; # returns a list reference
-
-#if you must have a copy of the referenced array, do this:
-my @clones = @$clones; # but you don't need to do this
-
-#get the first clone from the list via the reference:
-my $first_clone = $clones->[0];
-
-#another way of getting the same thing:
-($first_clone) = @$clones;
-
-#iterate through all of the contigs on a clone
-foreach my $contig (@{$first_clone->get_all_Contigs}) {
- print $contig->name . "\n";
-}
-
-#another way of doing the same thing:
-my $contigs = $first_clone->get_all_Contigs;
-foreach my $contig (@$contigs) {
- print $contig->name . "\n";
-}
-
-#retrieve a single Clone object (not a listref)
-$clone = $clone_adaptor->fetch_by_dbID(1);
-#no dereferencing needed:
-print $clone->id . "\n";
-
-
-
+EnsEMBL Tutorial
+by Michele Clamp (and revised/updated by Ewan Birney, Dan Andrews and Graham McVicker)
+
+Revisions: EB Oct01, MC Jan02, MC Mar02, DA Jul02, new API revision DA Oct02, GMV Oct02, DA Feb03
+
+Â_______________________________________________________________________
+
+Contents
+
+Introduction.
+Remote Access to EnsEMBL via MySQL.
+Introduction to the Perl object API.
+ Companion script and exercises.
+ What does the core EnsEMBL database contain?
+ Set-up.
+ Connection.
+ Business objects and object adaptors.
+ Let's get some data.
+ Clones and RawContigs.
+ Slices.
+ Slices vs RawContigs.
+ Sequence features.
+ Analysis objects
+ Overlaps
+ Genes.
+ Supporting evidence.
+ Prediction transcripts.
+ Translation.
+ Protein.
+ External databases - the general idea.
+ SNPs.
+ MarkerFeatures.
+ A final, but important, note to users of older versions of the EnsEMBL API - Lists and List References
+
+_______________________________________________________________________
+Introduction
+
+It is important to us that you should be able to access the data in EnsEMBL databases as easily as possible. To try and meet the different needs of vastly differing data users we have developed a number of interfaces to the EnsEMBL data. You are probably reading this document because the web interface, while being very pretty and fast, is purely a web interface and won't provide the kind of bulk information you are needing. If you are a bioinformatician who is Perl aware, the API that this document describes is going to be very useful. Not only can you retrieve data directly through your scripts, to either remote or local EnsEMBL databases, you can do quite a lot of data processing via the tools built into the API data types. However, a point that needs to be made now is that you most probably DON'T have to use this API if your data needs are relatively simple. Instead, you can use the purpose-built data-mining tool called EnsEMBL Mart. We don't want to chase you away, but if you haven't already looked at Mart (www.ensembl.org/EnsMart/) we enthusiastically encourage you to do so soon.
+
+Okay. If you are still here, good. Now we can talk about accessing EnsEMBL databases, both directly and through the Perl API. The following document covers two broad areas. The first section pertains to accessing the public EnsEMBL MySQL server (kaka.sanger.ac.uk) and running queries with your own SQL. The second and larger section will introduce you to the EnsEMBL core API.
+
+In general, we've noticed that the best learning route for developers starting to use EnsEMBL is as follows:
+
+* Play around with the data on kaka.sanger.ac.uk with just a MySQL client.
+* Start using the object layer API (Perl based) against kaka.sanger.ac.uk (you will need to download EnsEMBL and bioPerl software).
+* Install the database locally. You may as well install the web site locally while you are about it, as it is pretty easy to get up and running.
+
+Of course, you might want to jump straight to installing the EnsEMBL web site, or the delve into the Perl API. Take your pick.
+
+Alternatively, you need no longer feel compelled to use Perl to work directly with EnsEMBL databases. If you want to work in Java you should definitely have a look at the rapidly growing Java API (www.ensembl.org/java/). This is not a subject covered in this document, so if you are interested please have a look at the Java specific documentation.
+
+Currently, a rather hard thing to do is to use the EnsEMBL software system to generate features de novo (i.e. run the gene building process) from raw sequence. This is called the "pipeline". To illustrate this, there are presently numerous remote EnsEMBL web sites but only a couple of remote pipelines. We expect that documentation and understanding of the pipeline will improve as more and more people attempt to run it remotely. The documentation of the pipeline that does exist is stored in our CVS repository, which is externally accessible. Perhaps the easiest way to obtain these documents in through our web-CVS (http://cvsweb.sanger.ac.uk/cgi-bin/cvsweb.cgi/ensembl-doc/). You are unlikely to get the pipeline working 100% properly without a bit of help, so if you are heading in this direction let us know and say hello.
+
+For other EnsEMBL documentation, have a browse through the EnsEMBL web site (www.ensembl.org) and follow the "Documentation" link. This should lead you to a number of useful "big" documents, including the one you are presently reading and the web-site installation instructions.
+
+For more detailed documents click on the "Wiki" link from the "Documentation" page. Some of the documents available in the Wiki are up to date, some (most) are not - caution. Once you are in the Wiki pages you can use the 'search' button to find what you are looking for. You can also browse the archives of the ensembl-dev mailing list for discussions which may have already answered some of your questions. If after doing this you still can't find what you need, please feel free to post a question on the ensembl-dev@ebi.ac.uk mailing list. We respond quickly and are nice people that don't bite, even if you haven't bothered to read the documentation (where it exists).
+
+Finally, if you find something in this document that is not clear or is incorrect, we will be most grateful if you let us know on the EnsEMBL developers mailing list (ensembl-dev@ebi.ac.uk). If you are shy, you can also email me directly (dta@sanger.ac.uk), I don't bite either.
+
+Remote Access to EnsEMBL via MySQL.
+
+EnsEMBL provides an internet accessible host (kaka.sanger.ac.uk) with the latest databases. This means you can do a lot of work from an internet connected host solely by using "client" software.
+
+You probably have a MySQL client installed if you are running a standard linux distribution. Try:
+
+mysql -u anonymous -h kaka.sanger.ac.uk
+
+If MySQL is not installed, go to www.mysql.com to download the client.
+
+Inside the MySQL prompt you generally want to start with the database "homo_sapiens_core_9_30" (at time of writing this was the most recent build) and the database "ensembl_mart_9_1". There are also databases for other species - you should be able to use these in the same way as described here for human.
+
+Try the "show databases" SQL command to see all the databases that are available, followed by a "use" command similar to "use homo_sapiens_core_9_30" to choose the current/most recent database. Other databases are named like "homo_sapiens_lite_9_1" - the naming scheme following the convention of species tag, underscore, database type, underscore and release number. Have a look around to see what you can use.
+
+Starting with the "core" database (homo_sapiens_core_9_30 or the most recent update of this database), try some of these nice queries to get a taste of what is stored in the databases:
+
+Choose the current human core database:
+
+"USE homo_sapiens_core_9_30;"
+
+Retrieve the first two DDBJ/EMBL/GenBank entries stored in the database:
+
+"SELECT clone_id FROM clone LIMIT 2;"
+
+Retrieve the first two confirmed genes in the database:
+
+"SELECT * FROM gene LIMIT 2;"
+
+Retrieve the DDBJ/EMBL/GenBank entries from the first 5Mb of human chromosome 1. The assembly information is stored in the assembly table, hence we need to perform a join across the clone, contig, chromosome and assembly tables:
+
+ "SELECT DISTINCT(clone.name)
+ FROM assembly, contig, clone, chromosome
+ WHERE chromosome.name = '1'
+ AND chromosome.chromosome_id = assembly.chromosome_id
+ AND assembly.chr_end < 5000000
+ AND assembly.contig_id = contig.contig_id
+ AND clone.clone_id = contig.clone_id;"
+
+Each table has a unique, consistently-named identifier which can be used as foreign keys in other tables. The unique identifier column is always named tablename_id and contains integer values. For example, the unique identifier of the chromosome table is the contained in the chromosome_id column and the row with chromosome_id = 23 corresponds to the chromosome named 'X'.
+
+Retrieve the exons which lie within the first 200,000 base-pairs of chromosome 1:
+
+"SELECT exon.exon_id
+ FROM exon, assembly, chromosome
+ WHERE chromosome.name = '1'
+ AND chromosome.chromosome_id = assembly.chromosome_id
+ AND assembly.chr_end < 500000
+ AND assembly.contig_id = exon.contig_id;"
+
+(Note: Existing EnsEMBL users may notice that the exon.id column has been replaced by exon.exon_id which now contains an integer value. To get the ENSE number you will need to join to exon_stable. Be aware that the newest release of the EnsEMBL database represents quite a big shift in the schema compared to previous releases.)
+
+At this point you can see that constructing queries against the EnsEMBL core database in chromosomal coordinates will always mean joining to the assembly table. This is a bit painful. Thankfully we have developed a query-optimised database that is derived from this database (a datamart in trendy computer speak), called EnsEMBL Mart. This is changing rapidly, but is well worth playing around with:
+
+"USE ensembl_mart_9_1;"
+
+To genes from a particular region:
+
+"SELECT gene_stable_id FROM homo_sapiens_core_gene WHERE chr_name = '1' AND gene_chrom_end < 200000;"
+
+Or from a particular band:
+
+"SELECT gene_stable_id FROM homo_sapiens_core_gene WHERE band LIKE 'q25.%' AND chr_name = '1';"
+
+(notice the use of LIKE to truncate to the major band)
+
+EnsEMBL Mart will expand over time to allow progressively richer queries. To investigate more use the commands:
+
+"SHOW tables;"
+
+and
+
+"DESCRIBE tablename ;"
+
+So, now you can play with the data, but where are things like the translations or cDNAs? Additionally, how can I find all the BLAST hits which overlap exons that live in my genomic region of interest? To do this you will need to write code often. Do yourself a favour and take advantage of our API which we use every day to wrangle this kind of data.
+
+Introduction to the Perl object API
+
+This document grew from notes prepared for an introductory half-day course that we occasionally run on the EnsEMBL code base (0.5M lines of code in half a day - now there's value). It will take you through the process of connecting to an EnsEMBL database and accessing the data contained therein. The information contained here is by no means exhaustive, but should give you enough knowledge to be able to competently use the core EnsEMBL code. After learning how to retrieve genes, exons, sequence and process these to your own ends, we hope you will feel confident enough to delve deeper into other less-trodden parts of the code that may interest you.
+
+Before you start, you will need to have the relevant EnsEMBL and BioPerl modules installed. These are :
+
+bioperl-0.7 (we know this is not the latest version - for now, EnsEMBL is tied to it, so please use it.)
+ensembl
+ensembl-external
+
+Instructions on how to install these perl modules are contained on the EnsEMBL website www.ensembl.org. Basically, you need to do the following steps (in both cases below we are using cvs to get the code, which is much better than ftp as we are getting the latest bug fixes. Notice the - r flag to the cvs commands. These indicate the branch of each repository to get out. (Branches are stable versions of the code).
+
+ BioPerl (see cvs.bioperl.org for more details)
+cvs -d :pserver:cvs@cvs.bioperl.org:/home/repository/bioperl login
+when prompted, the password is 'cvs'
+cvs -d :pserver:cvs@cvs.bioperl.org:/home/repository/bioperl checkout -r branch-07 bioperl-live
+
+ EnsEMBL
+
+cvs -d :pserver:cvsuser@cvsro.sanger.ac.uk:/cvsroot/CVSmaster login
+when prompted, the password is CVSUSER
+cvs -d :pserver:cvsuser@cvsro.sanger.ac.uk:/cvsroot/CVSmaster checkout -r branch-ensembl-9 ensembl
+cvs -d :pserver:cvsuser@cvsro.sanger.ac.uk:/cvsroot/CVSmaster checkout -r branch-ensembl-9 ensembl-external
+
+If you don't have, or don't want to install, the EnsEMBL database locally (which is all you will need to complete the tutorial exercises) you can point your scripts at a publically available one at the Sanger Centre. Use the following fields in your scripts (where X_X is the latest version of the database):
+
+host kaka.sanger.ac.uk
+dbname homo_sapiens_core_X_X
+user anonymous
+
+ DBI and DBI::mysql
+
+Unless you already have them installed, before you can begin you will need to install the Perl DBI and DBI::mysql modules from the CPAN (www.cpan.org). See the CPAN site for instructions on how to do this.
+
+
+Companion script and exercises
+Accompanying this document is a script called tutorial.pl. This script contains all the example code in this document and should run successfully if you have the right database and version of the code installed. In addition, soon there will be worked solutions to the exercises included later in this tutorial. When you check-out a copy of EnsEMBL from the cvs, both the companion script and the pending worked solutions will be in the ensembl/docs/tutorial directory.
+
+What does the core EnsEMBL database contain?
+
+*Clones (with embl accessions) - both finished and unfinished.
+
+*Each contiguous piece of sequence is called a contig. These are the basic lengths of DNA that we analyse and annotate.
+
+*Each clone will contain one or more contigs.
+
+*Finished clones = one contig.
+
+*Unfinished clones = any number of contigs.
+
+*Each contig (whether finished or unfinished) has certain features associated with it that represent the result any one of the various analysis programs that have been run on it (e.g. RepeatMasker, BLAST, genscan). These are the basic computes used to build the genes.
+
+*The raw analysis results are used to build genes which are also stored in the database. Each gene contains one or more transcripts and each transcript will contain a translation.
+
+*Each gene has various information attached to it describing whether it is a known gene or corresponds to a SwissProt or trEMBL protein. Some genes are novel genes which have been built by inference from similarities to other sequences. These novel genes won't have a corresponding SwissProt or trEMBL protein.
+
+*Each translation has had a variety of protein analyses conducted on it and you can access information about the results of these. This includes information derived from pfam, prosite and prints.
+
+*There are other features accessible through non-core EnsEMBL external databases. Such databases may contain information about SNPs, mouse trace hits or embl annotations. You can create your own external databases to be incorporated into EnsEMBL, but this is a subject for a more advanced document.
+
+Setup
+
+Before starting with the EnsEMBL modules you will need to set up your environment so Perl knows where to find them. As EnsEMBL is built on top of BioPerl (version 0.7 for now), this includes telling it where bioperl-0.7 lives on your system.
+
+The environment variable to do this is PERL5LIB. If you are using csh or tcsh you need to type in the following (changing /nfs/croc/michele/branch to your directory containing the perl modules you downloaded). If you are concomitantly using a version of BioPerl newer than version 0.7 make sure that the older version appears in your Perl library path before the newer version.
+
+setenv ENSHOME /nfs/croc/michele/branch
+
+setenv PERL5LIB $ENSHOME/ensembl/modules:$ENSHOME/bioperl-0.7:$ENSHOME/ensembl-external/modules:$ENSHOME/ensembl-lite/modules
+
+It has become apparent recently that the EnsEMBL code-base just won't run on older versions of Perl. We use version 5.6 for development and testing. We recommend that you should do so too. If you are unsure of what version is running on your machine. Type:
+
+perl -v
+
+Presuming that this is all in order, you are now ready to write your first script.
+
+Connection
+
+All data used and created by EnsEMBL is stored in a MySQL relational database. If you want to access this database the first thing you have to do is to connect to it. This is done behind the scenes by EnsEMBL using the famous Perl module called DBI. You will need to know three things before you start :
+
+ host the hostname where the EnsEMBL database lives
+ dbname the name of the EnsEMBL database
+ user the username to access the database
+
+First, we need to declare to Perl the modules we want to use so it can go and check the syntax of them. This is done by a use statement. This line has to be inserted into all your EnsEMBL scripts:
+
+use Bio::EnsEMBL::DBSQL::DBAdaptor;
+
+The we set the all important variables telling Perl where and what your database is:
+
+my $host = 'kaka.sanger.ac.uk';
+my $user = 'anonymous';
+my $dbname = 'homo_sapiens_core_9_30';
+
+Now we can make a database connection:
+
+my $db = new Bio::EnsEMBL::DBSQL::DBAdaptor(-host => $host,
+ -user => $user,
+ -dbname => $dbname);
+
+We've made a connection to an EnsEMBL database and passed parameters in using the -attribute => 'somevalue' syntax present in many of the EnsEMBL object constructors. Formatted correctly, this syntax lets you see exactly what arguments and values you are passing.
+
+The $db variable now contains a reference to the newly created DBAdaptor object which you can start using to extract data. If, heaven forbid, the connection fails an error message will be returned.
+
+Business Objects and Object Adaptors
+
+Before we launch into the ways the API can be used to retrieve and process data from the EnsEMBL databases it is best to mention the fundamental relationships the EnsEMBL objects have with the database.
+
+The EnsEMBL API allows manipulation of the database data through various business objects. For example, some of the more heavily used objects are the Gene, Clone and Exon objects. More details of how to effectively use these objects will be covered later. These objects are retrieved and stored in the database through the use of object 'adaptors'. The object adaptors have internal knowledge of the underlying database schema and use this knowledge to fetch, store and remove objects (and data) from the database. This way you can write code and use the EnsEMBL API without having to know anything about the underlying databases you are using. The database adaptor (that we obtained by connecting to our database, in the previous section) is a special adaptor which has the responsibility of maintaining the database connection and creating other object adaptors.
+
+As will be shown later in this document, to obtain object adaptors from the main database adaptor there is a suite of methods that have the naming convention "get_ObjectAdaptor". To obtain a SliceAdaptor or a CloneAdaptor (which, oddly enough, retrieve Slice and Clone objects) do the following:
+
+my $gene_adaptor = $db->get_GeneAdaptor();
+my $clone_adaptor = $db->get_CloneAdaptor();
+Don't worry if you don't immediately see how useful this could be. Just remember that you don't need to know anything about how the database is structured, but you can retrieve the necessary data (neatly packaged in objects) by asking for it from the correct adaptor. Throughout the rest of this document we are going to work through the ways the EnsEMBL business objects can be used to derive the information you want.
+
+Let's get some data
+
+Once you have a database adaptor, you are ready to retrieve data. Conceptually, there are two main ways to go about retrieving and manipulating genome data. Historically, data has been handled on a clone- by- clone basis. The EnsEMBL API is fully capable of working with the clone- based data that comes out of a sequencing project, and fundamentally EnsEMBL works with data at a contig level. However, due to the nearing completion of the first big genome sequencing projects it is now becoming possible to practically work with genomes as long stretches of gap-less sequence. EnsEMBL also allows you to work with sequence information in this way. Previous releases of the EnsEMBL API called these un-interrupted stretches of sequence virtual contigs. From release 9 onwards these have been called Slices - literally, slices of genomic sequence. All you need to do to retrieve such a sequence is stipulate the chromsome number and the start and end coordinates - we'll get to doing this in just a bit.
+
+Most users of the EnsEMBL API will probably end up using both means of retrieving and manipulating genome sequences. Hence, we'll introduce both of these here in the next two sections.
+
+
+Clones and RawContigs
+
+Clones and RawContigs represent two basic data-types in EnsEMBL. Clones have contigs, and contigs are the elements of contiguous sequence information that are built up to create the full clone sequence. Once a clone sequence is complete, the clone will be represented by one contig sequence.
+
+If you know the accession number of a clone that you are interested in, you can retrieve that clone directly from the CloneAdaptor in the following way:
+
+my $clone = $clone_adaptor->fetch_by_accession('AC005663');
+
+This will return a Clone object. You can check whether you have the right clone by calling the Clone object's id method:
+
+print "Clone is " . $clone->id . "\n";
+
+To get the most use out of this object we now need to ask it about its contigs (remember - clones have one or more contigs).
+
+my $contigs = $clone->get_all_Contigs;
+
+We now have a reference to a list of RawContig objects. RawContig objects are actually bioperl sequence objects and this means they can be used to retrieve all sorts of useful information,
+
+Say we want to get the sequence of each contig:
+
+foreach my $contig (@$contigs) {
+ my $length = $contig->length;
+ my $id = $contig->id;
+
+ # The sequence of a contig is obtained easily:
+
+ print $contig->seq . "\n";
+
+ # We can get a substring of this sequence too:
+
+ print $contig->subseq(1,100) . "\n";
+
+ # If we want to write the sequence to a file we use bioperl again
+ # (Note: if we are creating a new object we need to include
+ # a use Bio::SeqIO line at the start of our file
+
+ use Bio::SeqIO;
+
+ my $seqio = new Bio::SeqIO(-fh => \*STDOUT,
+ -format => 'fasta');
+
+ $seqio->write_seq($contig);
+
+}
+
+Many features can be obtained from a RawContig. One set of features that will prove extremely useful if you're going to do any analysis on them are the repeat features. These can be used to mask the sequence ready for, say, a BLAST search. For example:
+
+my $maskedseq = $contig->get_repeatmasked_seq;
+
+Hence, EnsEMBL provides you with a handy call to obtain repeat-masked sequence without you having to rerun RepeatMasker.
+
+Of course, given a whole genome sequence to play with, you are hardly going to be content with looking at just one clone. Hence, to do genome sized analyses you will probably want to retrieve all the clones from the database. Before going further it is best to point out that unless you have an over-riding reason to work on a clone-by-clone basis, this kind of analysis might be more easily done using Slices, which are the subject of the next section. However, if you need clones, you can retrieve them in the following way.
+
+my $clones = $clone_adaptor->fetch_all;
+
+Once this list of clones has been obtained you can set about getting useful things.
+
+foreach my $clone (@$clones) {
+ my $contigs = $clone->get_all_Contigs;
+ foreach my $contig (@$contigs) {
+ my $sequence = $contig->seq();
+ print $sequence . "\n";
+ }
+}
+
+Notice that the above script allows you to print the entire genome sequence, which is potentially quite slow. Unless you have a reason to laboriously work through clone and contig sequences, you will be much better off using Slices - which we will come to just around the next corner.
+
+Exercises 1
+
+1. Connect to the database. How many clones does it contain?
+
+ (Hint: Make a $db object and get a clone adaptor from it. Use the fetch_all() method of the adaptor and then take the size of returned array).
+
+2. What is the average number of raw contigs per clone for the first 100 clones in the database?
+
+ (Hint: get_all_Contigs on clone);
+
+3. Print out in fasta format the repeat-masked sequence for the last 10 clones of the genome.
+
+
+Slices
+
+If you have had experience with using the EnsEMBL API before, you will have come across the concept of virtual contigs. A virtual contig was an EnsEMBL object that represented a section of a genome. These virtual contigs could be created to represent any single part of the genome, and were created simply by making a call to the $db object specifying start and end coordinates for a particular chromosome. The nitty gritty of taking the actual raw contig sequences and stitching these together into a single sequence was done in the background by the EnsEMBL code. The current incarntion of a virtual contig is the Slice - virtual contigs are no longer part of the EnsEMBL code. Just like virtual contigs a Slice is an EnsEMBL object that represents any particular region of a genome. A Slice and its associated methods provide a simple way to retrieve and manipulate sequence from a part of the genome that you are interested in. To get a Slice of genome you just need to do the following.
+
+First, get a Slice adaptor from the database adaptor:
+
+my $slice_adaptor = $db->get_SliceAdaptor;
+
+With a Slice adaptor, retrieving genome sequence is as easy as specifying which region of which chromosome we are after:
+
+my $slice = $slice_adaptor->fetch_by_chr_start_end('1',1,100000);
+
+The order that the arguments are passed is chromosome number, start coordinate and end coordinate. Notice that this is reflected in the naming of the slice adaptor method. Generally this is a good guide to guessing what arguments you need to provide a method call - alternatively you can have a look at the documentation associated with a particular module.
+
+Another useful way to obtain a Slice is to do it with respect to a gene it contains:
+
+my $slice = $slice_adaptor->fetch_by_gene_stable_id('ENSG00000099889', 5000);
+
+This will return a Slice that contains the sequence of the gene specified by its stable EnsEMBL id. It also returns 5000bp of flanking sequence at both the 5' and 3' ends, which is obviously very useful if you are interested in the environs that a gene inhabits. You needn't have the flanking sequence it you don't want it - in this case set the number of flanking bases to 0 or omit the second argument entirely.
+
+We can also retrieve Slices of actual physical contigs - and we can pad these at the ends with sequence from adjoining contigs:
+
+my $slice = $slice_adaptor->fetch_by_contig_name('AC005663.2.1.103122', 10000);
+
+This returns a Slice that contains the contig that was specified by name. It also includes 10000bp of flanking sequence at each end of this actual contig.
+
+We can also do the same with clones:
+
+my $slice = $slice_adaptor->fetch_by_clone_accession('AC005663',1000);
+
+So, once we have our Slice we can then use it to retrieve useful information about the region of sequence that the Slice represents.
+
+Just like RawContigs mentioned previously, a Slice is a BioPerl sequence object and we we can get at the actual sequence information like thus:
+
+my $sequence = $slice->seq;
+
+We can query the Slice for information about itself:
+
+my $chr_name = $slice->chr_name;
+my $chr_start = $slice->chr_start;
+my $chr_end = $slice->chr_end;
+
+print "Chromosome $chr_name Start/End $chr_start $chr_end \n";
+
+The makeup of our Slice is determined by the assembly (or the golden tiling path) of our genome. If we wanted to access this assembly information with regard to a particular Slice we can do this by:
+
+my $tiling_path = $slice->get_tiling_path();
+
+foreach my $tile (@$tiling_path) {
+ my $tile_slice = $tile->assembled_Seq()->name;
+ my $tile_ctg = $tile->component_Seq()->name;
+
+ my $slice_start = $tile->assembled_start();
+ my $slice_end = $tile->assembled_end();
+ my $ctg_start = $tile->component_start();
+ my $ctg_end = $tile->component_end();
+ my $ctg_ori = $tile->component_ori();
+
+ print "[$tile_slice] $slice_start - $slice_end => " .
+ "[$tile_ctg] $ctg_start - $ctg_end ($ctg_ori)\n";
+}
+
+Each 'tile' that we are returned is a Bio::EnsEMBL::Tile object representing the portion of a slice that maps directly onto a portion of the assmebly (or golden path).
+
+A Slice is a good starting place for retrieving information about a region of a chromosome, including the genes it contains. The next few sections will go into more detail about how to do this.
+
+
+Slices vs RawContigs
+
+Before going on to the next topics an important point needs to be made. Despite the fact that Slices and RawContigs are different objects and don't represent exactly the same thing (one is an experimentally generated stretch of sequence, the other is a computationally pooled sequence) they can effectively be used as such. In the rest of this document a distinction won't be drawn between the two - unless there is an actual difference between how the two objects behave (which by design should be few). We will be using Slices by default though, as we would encourage you to do too.
+Exercises 2
+1. Fetch 1Mb of repeat-masked sequence from the contig of your choice (Hint: Create a Slice using fetch_Slice_by_chr_start_end($chr,$start,$end) and have a look back at how this was done with RawContigs in the previous section).
+2. Fetch a Slice of the gene ENSG00000100259
+3. Print the last 1000 nucleotides of the Silce obtained in the previous question.
+3. For the Slice you retrieved in the first question, print the ids of the contigs that form the tiling path. Also print the start and end positions of each RawContig as it is used in the tiling path.
+
+Sequence Features
+
+So now we're pretty happy about retrieving DNA sequence from EnsEMBL. The more interesting things associated with Slices and RawContigs are the features that can be retrieved from them. These include the repeat features mentioned in previously and also similarity features (like BLAST results), prediction transcripts (such as genscan results) and marker features. Not to mention genes, of course.
+
+Sets of features can be retrieved from a region through a request to a Slice or RawContig (you're probably getting used to this by now). These are returned to us as feature *objects*. For instance:
+
+my $repeats = $slice->get_all_RepeatFeatures;
+
+We now have a reference to a list containing feature objects. An easy way to print these out is to call the method gffstring which returns information about the feature as a string:
+
+foreach my $repeat (@$repeats) {
+ print $repeat->gffstring . "\n";
+}
+
+You should get a series of output lines like
+
+22:16890204-16987127 RepeatMasker repeat 2851 2959 645 + -1
+22:16890204-16987127 RepeatMasker repeat 2979 3260 2066 - -1
+22:16890204-16987127 RepeatMasker repeat 4008 4056 234 - -1
+22:16890204-16987127 RepeatMasker repeat 4080 4345 1768 - -1
+22:16890204-16987127 RepeatMasker repeat 4364 4450 549 - -1
+22:16890204-16987127 RepeatMasker repeat 4650 4736 252 - -1
+22:16890204-16987127 RepeatMasker repeat 5835 5972 413 - -1
+22:16890204-16987127 RepeatMasker repeat 12035 12084 193 - -1
+
+The columns that compose a gffstring have the following meaning:
+
+1 sequence name
+2 feature type
+3 main feature type
+4 sequence start
+5 sequence end
+6 strand
+7 score
+8 phase
+
+Sometimes there are an additional three columns if the feature type posesses them:
+
+9 hit sequence name
+10 hit start
+11 hit end
+
+
+Instead of using gffstring we can ask the feature objects directly about their properties:
+
+foreach my $repeat (@$repeats) {
+ print "Name : " . $repeat->seqname . "\n";
+ print "Start : " . $repeat->start . "\n";
+ print "End : " . $repeat->end . "\n";
+ print "Strand : " . $repeat->strand . "\n";
+ print "Score : " . $repeat->score . "\n";
+
+}
+
+Some features (like CpG islands for instance) are simple features and only have the methods printed above.
+
+Other features as well as having coordinates on a contig sequence also have coordinates on a hit sequence:
+
+foreach my $repeat (@$repeats)
+ print "Hit start " . $repeat->hstart . "\n";
+ print "Hit end " . $repeat->hend . "\n";
+}
+
+Repeat features are an example of a feature which also stores the coordinates of a hit sequence. However, the classic example is a feature representing a BLAST result where the query sequence is similar to another sequence (protein, EST, cDNA) and so we need to store which sequence it has hit and whereabout in that sequence it has hit. These types of features have the generic name of similarity features. In reality these features are members of a superclass called AlignFeatures (because they are features with two aligned sequences), and depending on whether it is DNA or protein sequence that we are dealing with, the objects are either a Bio::EnsEMBL::DnaDnaAlignFeature or a Bio::EnsEMBL::ProteinDnaAlignFeature
+
+To get similarity features from a RawContig or a slice we use:
+
+my $features = $contig->get_all_SimilarityFeatures;
+
+To obtain only DnaAlignFeatures we can use the following method:
+
+$features = $slice->get_all_DnaAlignFeatures('Vertrna', 0.00001);
+
+Note that this method takes two optional arguments which can impose an additional constraint on the features we want to retreive. The first argument is a 'logic name' - the name for the analysis which was conducted to obtain the feature. For similarity features the logic name relates to the type of blast analysis and there are currently four logic_names which correspond to dna_align_features in the current code (note that the logic name is CASE SENSITIVE):
+
+Vertrna WU tblastn hits against the EMBL-vertrna database (all CDS's from the EMBL database).
+Swall WU blastp hits against the SWALL database (protein features only).
+Unigene WU tblastn hits against the UniGene database.
+dbEST WU tblastn hits against the dbEST database.
+
+Hence, in our call to retrieve DNA features, we have requested those that are hits against the EMBL vertrna database. We have also stipulated a cut- off value. This allows us to set the level of strigency of BLAST hits and is exactly the same as the BLAST E(0) value.
+
+Once we have these DNA features we can iterate through them and take a look at what they contain:
+
+foreach my $feat (@$features) {
+ print "Feature scores are " . $feat->score . "\t" .
+ $feat->percent_id . "\t" .
+ $feat->p_value . "\n";
+}
+
+The same procedure applies to retrieving protein features, just instead use the method call:
+
+$slice->get_all_ProteinAlignFeatures('Swall', 0.0001).
+
+Choose a logic name appropriate to proteins - so stick with 'Swall' (or leave the logic name out entirely). Once these protein features have been returned we can look at them in the same way as we just did for DNA features.
+
+As an alternative approach we can go directly to the object adaptor for a feature type and request features from the database. The following example illustrates some of the different approaches that can be used to obtain features:
+
+my $simple_feature_adaptor = $db->get_SimpleFeatureAdaptor;
+
+#get all of the Eponine hits on a Contig
+my @eponine_hits = @{$simple_feature_adaptor->fetch_all_by_RawContig($contig, 'Eponine')};
+
+#get all CpG islands on a Slice with score greater than 500
+my $cpg_islands = $simple_feature_adaptor->fetch_all_by_Slice_and_score($slice, 500, 'CpG');
+
+#get the simple feature with the database identifier 1
+my $simple_feature = $simple_feature_adaptor->fetch_by_dbID(1);
+
+#get all of the simple features on a slice
+my @simple_features = @{$slice->get_all_SimpleFeatures};
+
+#get all of the tRNAscan hits on a contig
+my $trnas = $contig->get_all_SimpleFeatures('tRNAscan');
+
+Analysis Objects
+
+All features retrieved from the database have an Analysis object attached to them that contains information regarding the analysis/algorithm that created the feature. This is a good means by which to determine where the feature has come from or what it actually is. The gff_source method of the Analysis object tells us what sort of feature we have, such as 'genscan','repeat' or 'cpg'. Other information can be derived from the Analysis object, such as the database that was used in a BLAST job, the parameters that were provided to the executable, and even the path to the executable that was used:
+
+my $analysis = $feature->analysis;
+
+print "Analysis: " . $analysis->gff_source . "\n";
+print "Database : " . $analysis->db . "\n";
+print "Program : " . $analysis->program . "\n";
+print "Parameters : " . $analysis->parameters . "\n";
+
+For instance a feature that comes out of a BLAST run will have an analysis object that tells us it was run with 'blastx' and was run against database 'sptr';
+
+Overlaps
+
+A useful feature of BioPerl is that it makes it easy to find whether one feature overlaps another. This comes in jolly handy if you want to find all genscan predictions that don't overlap exons, or all SNPs that do overlap exons, or mouse trace hits that don't overlap exons, etc.
+
+If we have two features - say an exon, $exon, and a snp, $snp:
+
+ if ($exon->overlaps($snp)) {
+ print "Whey! coding snp " . $snp->gffstring . "\n";
+ } else {
+ print "Boo! non coding snp " . $snp->gffstring . "\n";
+ }
+
+The overlaps method returns true (1) if the two features do overlap, or false (0) if they don't.
+
+Exercises 3
+
+1. Print out all the repeat features for the first 100kb of sequence of human chromsome 1. (Hint: Use the gffstring method for easy printing)
+
+2. What proportion of DNA is repeat in the above sequence and is this what you expect? (Hint: tot up the length for each repeat feature and compare to the total sequence length)
+
+3. For clone AC005663 retrieve all the DNA features matched to EMBL vertrna. How many different sequences did this clone hit and were these hits significant? (Hint: Use the hseqname method and the p_value method)
+
+Genes
+
+During the pipeline gene building process, genes are built on Slices because a lot of genes span more than one contig. Hence , it makes the most sense to subsequently use Slices to access them. However, having said that you can access genes via RawContigs if you wish. Getting all the of the genes from a Slice or RawContig is as easy as:
+
+my $genes = $slice->get_all_Genes;
+
+As usual we are returned a reference to a list of objects - Gene objects this time. They contain all the information about the exon/intron structure, DNA sequence, etc.
+
+Genes have EnsEMBL identifiers which can be accessed using the stable_id method:
+
+foreach my $gene (@$genes) {
+ print "Gene : " . $gene->stable_id . "\n";
+}
+
+EnsEMBL identifiers don't really tell us much about the gene (and they're not intended to) and some genes will have one or more more common names.
+
+We can tell immediately if a gene is a known gene (refseq or sptrembl) by calling the is_known method. If it is a known gene then we can call the each_DBLink method to find out more about it.
+
+foreach my $gene (@$genes) {
+ if ($gene->is_known) {
+ foreach my $link (@{$gene->get_all_DBLinks}) {
+ print "Gene " . $gene->stable_id . " links to " .
+ $link->display_id . " " .
+ $link->database . "\n";
+ my @syns = @{$link->get_all_synonyms};
+ print "Synonyms for gene are @syns\n";
+ }
+ } else {
+ print "Gene " . $gene->stable_id . " is not a known gene\n";
+ }
+}
+
+Other information about a known gene is contained in its description method. This information is extracted from the relevant swissprot or refseq entry.
+
+my $description = $gene->description;
+
+Genes are quite complicated objects and are constructed thusly.
+
+Each Gene object has one or more Transcript objects (one for each alternatively spliced cDNA).
+
+my $transcripts = $gene->get_all_Transcripts();
+
+Each Transcript is made up of a series of Exons. We can access the Exons and retrieve their sequence and coordinates.
+
+foreach my $exon (@{$transcript->get_all_Exons}) {
+ print "Found exon " . $exon->stable_id . " " .
+ $exon->start . " " .
+ $exon->end . " " .
+ $exon->seq->seq;
+}
+
+Notice that calling the seq method on an Exon object returns us a BioPerl sequence object. We then have to call seq again to get a string representation of the sequence. Hence, we get the seq->seq stutter - its not a typo in case you were wondering.
+
+We can get the protein sequence of a Transcript by calling the translate method. So to get all the protein translations from a gene into a file we would do
+
+my $seqio = new Bio::SeqIO->(-format => 'fasta',
+ -fh => \*STDOUT);
+
+foreach my $transcript (@{$gene->get_all_Transcripts}) {
+ my $peptide = $transcript->translate;
+
+ $seqio->write_seq($peptide);
+}
+
+Note that when writing to a BioPerl SeqIO object we pass the Peptide object and not a string, but when writing out to the screen we have to stringify it first.
+
+Exercises 4
+1. Get all the genes on a long- ish (say 1Mb) Slice and print their EnsEMBL ids.
+
+2. Which of those genes are known genes and what is their more common name? (Hint. Use the is_known method and use the get_all_DBLinks->display_id)
+
+3. How many genes are alternatively spliced for the first 100 genes (Hint: Count the number of transcripts using the $gene->each_Transcript method)
+
+4. What is the average size and number of exons per gene (Hint: Use the get_all_Exons method and remember that exons are like features with $ex->start $ex->end)
+
+5. Translate the first 10 genes - are there any stop codons (there shouldn't be!!) (Remember there may be more than one transcript per gene)
+
+6. Print out the 200 bases of sequence that flanks each exon (Use the $gene->get_all_Exons method and call $contig->subseq($start,$end) method to retrieve the dna)
+
+7. Extract all the introns from the first 10 genes and write them to a file (be careful about reverse strand genes).
+
+8. Print out all the 5' and 3' utrs for the first 10 genes.
+Supporting Evidence
+
+The information that was used to make a gene is also stored in the EnsEMBL database in the form of FeaturePairs. This information can be retrieved easily from a Gene object.
+
+The evidence is attached as an array of FeaturePairs to each Exon and can be retrieved in the following way:
+
+foreach my $gene (@$genes) {
+ my $transcripts = $gene->get_all_Transcripts;
+ foreach my $transcript (@$transcripts) {
+ my $exons = $transcript->get_all_Exons;
+ foreach my $exon (@$exons) {
+ my $supporting_features = $exon->get_all_supporting_features;
+ foreach my $feature (@$supporting_features) {
+ print "Evidence " . $feature->gffstring . "\n";
+ }
+ }
+ }
+}
+
+Prediction Transcripts
+
+EnsEMBL stores ab initio gene predictions from genscan. These predictions are accesses in a similar fashion to normal EnsEMBL transcripts , but through PredictionTranscript objects rather than Transcript objects. As with normal transcripts prediction transcripts are composed of a set of Exon objects. To obtain prediction transcripts from a Slice or RawContig we can do the following (as with other EnsEMBL features logic name or score constraints can be passed as optional arguments) :
+
+my $predicted_transcripts = $slice->get_all_PredictionTranscripts;
+
+We now have a reference to a list of PredictionTranscript objects. To have a look at the predicted exons for each predicted transcript we can use the following:
+
+foreach my $predicted_transcript (@$predicted_transcripts) {
+ my $exons = $predicted_transcript->get_all_Exons;
+
+ print "Genscan prediction has " . scalar @$exons . : exons\n";
+
+ foreach my $exon (@$exons) {
+ print $exon->start . " - " .
+ $exon->end . " : " .
+ $exon->strand . " " .
+ $exon->phase ."\n";
+ }
+}
+
+As these predictions should translate there you can get the predicted peptide sequence by using the translate method on a genscan prediction.
+
+print "Genscan peptide is " . $predicted_transcript->translate->seq . "\n";
+
+Translation
+
+Each Transcript, retrieved from a Gene object , can be split into a 5' untranslated region, a CDS and a 3' untranslated region. The points in the cDNA where the translation starts and stops are stored in a Translation object which is attached to each Transcript:
+
+my $translation = $transcript->translation;
+
+The Translation object has methods:
+
+$translation->start_Exon;
+$translation->end_Exon;
+
+which denote which exons the translation starts and ends in. To find the exact coordinate of the start and stop of translation use the methods:
+
+$translation->start;
+$translation->end;
+
+The start and end methods refer to the exon coordinates so they should never be less than one or greater than the exon's length.
+
+Protein
+
+Information regarding a protein can be retreived from the ProteinAdaptor via a Translation object identifier:
+
+my $protein_adaptor = $db->get_ProteinAdaptor;
+
+my $protein = $protein_adaptor->fetch_by_translation_id($translation->dbID);
+
+As every transcript has only one translation we can fetch proteins using the transcript identifier as well:
+
+my $protein = $protein_adaptor->fetch_by_transcript_id($transcript->dbID)
+
+Once we have a Protein object we can look at its features
+
+my $protein_features = $protein->get_all_ProteinFeatures;
+
+The features that are returned are ProteinFeatures. To have a look at them you can use the gff_string method:
+
+foreach my $pf (@$protein_features) {
+ print $pf->gffstring . "\n";
+}
+exercises 5
+
+1. Find all the pfam domains contained in the first 100 genes on chromosome 1. Which ones are most common and is this surprising?
+
+2. How many of those genes have no protein features at all.
+
+3. How many of the proteins contain WD40 domains?
+
+External Databases - The General Idea
+
+The core EnsEMBL database (the one you've been using up to now) contains DNA, genes and some sequence features. There are extra satellite databases that contain other features that can also be accessed.
+
+The idea is that you take your main EnsEMBL database handle ($db - way back in the first section) and give it another database handle to look after. This external database could contain all manner of things e.g. SNPs, mouse trace hits or EMBL annotations. You can now access the features in the second database as though they were all in the same place even though they could be sitting on a completely different machine.
+
+Let's give the EST database as a first example.
+
+First we need to connect to the main EnsEMBL database:
+
+use Bio::EnsEMBL::ExternalData::ESTSQL::DBAdaptor;
+
+my $est_db = Bio::EnsEMBL::ExternalData::ESTSQL::DBAdaptor->new(
+ -host => $host,
+ -dbname => $estname,
+ -user => $user);
+
+And then to the human EST database to our main db adaptor and vice-versa:
+
+$db->add_db_adaptor('est', $est_db);
+$est_db->add_db_adaptor('core', $db);
+
+Now that we have done this we can retrieve the EST alignment features from this the database in the same way as we would other DNA align features by specifying the name of the analysis that was used to generate the alignments - in this case 'ex_e2g_feat'.
+
+my $est_features = $slice->get_all_DnaAlignFeatures('ex_e2g_feat');
+
+Now that we have these features we can have a look at them:
+
+foreach my $est (@$est_features) {
+ print " " . $est->gffstring . "\n";
+}
+
+SNPS
+
+SNP features can be readily accessed from the external database ensembl-lite:
+
+use Bio::EnsEMBL::Lite::DBAdaptor;
+
+my $lite_db = Bio::EnsEMBL::Lite::DBAdaptor->new(-host => $host,
+ -user => $user,
+ -dbname => $litename);
+
+Add the lite db adaptor to the main db adaptor and vice-versa like before:
+
+$db->add_db_adaptor('lite', $lite_db);
+$lite_db->add_db_adaptor('core', $db);
+
+Retrieve all the SNP features from a Slice using the get_all_SNPs method:
+
+my $snp_features = $slice->get_all_SNPs;
+
+Iterate through the SNP features printing the position at which they lie along the Slice:
+
+foreach my $snp (@$snp_features) {
+ print "snp " . $snp->start . "\n";
+}
+
+
+Marker Features
+
+The lite database also contains information about markers. Given that we already have a warm ensembl-lite database adaptor, it is too much to resist retrieving these too.
+With one call we can retrieve all the marker features on our Slice:
+
+my @landmark_features = @{$slice->get_all_landmark_MarkerFeatures};
+
+Exercises 6
+
+1. Connect to the core EnsEMBL database and the SNP database. Add the SNP database to the EnsEMBL database and vice-versa.
+
+2. Create a Slice of 1Mb of sequence from your chromosome of choice (hint - remember back to the Slice exercises)
+
+3. How many SNPs are there in this region?
+
+4. How many SNPs overlap exons? (Hint - get all genes from the Slice and use get_all_Exons to get the exons from the genes. Use the overlaps method to calculate the proportion of SNPs that are situated in coding sequences.)
+
+
+A final, but important, note to users of older versions of the EnsEMBL API - Lists and List References
+
+The EnsEMBL API has just recently passed through a rapid period of change. A significant difference between old and new versions of the API is the now widespread use of list references (listrefs). Some method calls can potentially return very long lists and it is far more efficient, both time- and memory-wise, for these methods to return references to these lists instead. Although not all methods return large arrays of objects, for consistency almost all methods in the API should now return list references rather than lists/arrays. A naming standard has also been developed which should make it easier for API users to surmise which methods return a single value or return a list reference. Business object methods with the prefix get_all_ return a list reference, as do adaptor methods with the prefix fetch_all_ . If you are returned a listref from a method call it is a simple matter to de-reference this. The following examples illustrate the use of list references in the EnsEMBL API:
+
+#fetch all clones from the clone adaptor
+my $clones = $clone_adaptor->fetch_all; # returns a list reference
+
+#if you must have a copy of the referenced array, do this:
+my @clones = @$clones; # but you don't need to do this
+
+#get the first clone from the list via the reference:
+my $first_clone = $clones->[0];
+
+#another way of getting the same thing:
+($first_clone) = @$clones;
+
+#iterate through all of the contigs on a clone
+foreach my $contig (@{$first_clone->get_all_Contigs}) {
+ print $contig->name . "\n";
+}
+
+#another way of doing the same thing:
+my $contigs = $first_clone->get_all_Contigs;
+foreach my $contig (@$contigs) {
+ print $contig->name . "\n";
+}
+
+#retrieve a single Clone object (not a listref)
+$clone = $clone_adaptor->fetch_by_dbID(1);
+#no dereferencing needed:
+print $clone->id . "\n";
+
+
diff --git a/docs/tutorial/tutorial.pl b/docs/tutorial/tutorial.pl
index 878351b212..f4b5d2825c 100755
--- a/docs/tutorial/tutorial.pl
+++ b/docs/tutorial/tutorial.pl
@@ -1,4 +1,4 @@
-#!/usr/local/bin/perl
+#!/usr/local/bin/perl -w
# This file is the companion script to the ensembl_tutorial and has been
# tested with the homo_sapiens_core_9_30 database on ecs1d and the main trunk
@@ -10,7 +10,6 @@
# MC Jan 2002
# Updated to main trunk code - Dan Andrews Oct 2002
# Re-updated to main trunk code - Graham McVicker Oct 2002
-# Updated to branch 16 - Michele Clamp Sept 2003
use Bio::EnsEMBL::DBSQL::DBAdaptor;
use Bio::EnsEMBL::Lite::DBAdaptor;
@@ -22,11 +21,11 @@ use strict;
### Currently there is no trunk db on kaka.
my $host = 'kaka.sanger.ac.uk';
my $user = 'anonymous';
-my $dbname = 'homo_sapiens_core_16_33';
+my $dbname = 'homo_sapiens_core_9_30';
### External dbs
-my $estname = 'homo_sapiens_est_16_33';
-my $litename = 'homo_sapiens_lite_16_33';
+my $estname = 'homo_sapiens_est_9_30';
+my $litename = 'homo_sapiens_lite_9_30';
#connect to the core database
@@ -337,10 +336,10 @@ $lite_db->add_db_adaptor('core', $db);
print "snp " . $snp->start . "\n";
}
-#print out marker features on a slice
-my @markers = @{$slice->get_all_MarkerFeatures};
+#print out landmark marker features on a slice
+my @landmark_features = @{$slice->get_all_landmark_MarkerFeatures};
-foreach my $marker (@markers) {
+foreach my $marker (@landmark_features) {
print $marker->gffstring . "\n";
}
diff --git a/misc-scripts/chain/chain2assembly.pl b/misc-scripts/chain/chain2assembly.pl
new file mode 100644
index 0000000000..87bda996bf
--- /dev/null
+++ b/misc-scripts/chain/chain2assembly.pl
@@ -0,0 +1,131 @@
+use strict;
+use warnings;
+
+use Getopt::Long;
+
+
+
+my ($seq_region_in, $chain_in);
+
+
+GetOptions('seq_region_in=s' => \$seq_region_in,
+ 'chain_in=s' => \$chain_in);
+
+$seq_region_in || usage();
+$chain_in || usage();
+
+open(SEQ_REGION_IN, $seq_region_in) or die("Could not open file $seq_region_in");
+open(CHAIN_IN, $chain_in) or die("Could not open file $chain_in");
+
+my %seq_region_map;
+
+my @row;
+while(<SEQ_REGION_IN>) {
+ chomp;
+ @row = split("\t", $_);
+ $seq_region_map{$row[1]} = $row[0];
+}
+
+close SEQ_REGION_IN;
+
+
+my $is_header_line = 1;
+my ($asm_sr_id, $cmp_sr_id, $asm_start, $cmp_start, $asm_strand, $cmp_strand,
+ $ori, $match, $insert, $delete, $asm_begin, $asm_end, $cmp_begin,
+ $cmp_end, $align_id, $asm_sr_len, $cmp_sr_len);
+
+LINE:
+while(<CHAIN_IN>) {
+ chomp;
+
+ if($is_header_line) {
+ my @header = split;
+
+ my $asm_sr_name = $header[2];
+ my $cmp_sr_name = $header[7];
+ $asm_sr_name =~ s/chr//; #take the 'chr' off of chromosome names
+ $cmp_sr_name =~ s/chr//;
+ $asm_sr_id = $seq_region_map{$asm_sr_name};
+ $cmp_sr_id = $seq_region_map{$cmp_sr_name};
+
+ if(!$asm_sr_id) {
+ warn("Unknown asm_seq_region $asm_sr_name");
+ $is_header_line = 0;
+ next LINE;
+ }
+ if(!$cmp_sr_id) {
+ warn("Unknown cmp_seq_region $cmp_sr_name");
+ $is_header_line = 0;
+ next LINE;
+ }
+
+ $asm_sr_len = $header[3];
+ $cmp_sr_len = $header[8];
+
+ $asm_strand = ($header[4] eq '+') ? 1 : -1;
+ $cmp_strand = ($header[9] eq '+') ? 1 : -1;
+ $ori = $asm_strand * $cmp_strand;
+
+ #start is (length-start) if we are on negative strand
+ #start coordinates in file start at 0 not 1
+ $asm_start = ($asm_strand == 1) ? $header[5]+1 : $asm_sr_len-$header[5];
+ $cmp_start = ($cmp_strand == 1) ? $header[10]+1 : $cmp_sr_len-$header[10];
+
+ $is_header_line = 0;
+
+ $align_id = $header[12];
+ } else {
+ if($_) {
+ next LINE if(/^\#/); #skip comments
+
+ next LINE if(!$asm_sr_id || !$cmp_sr_id);
+
+ ($match,$insert,$delete) = split;
+
+ if($asm_strand == 1) {
+ $asm_begin = $asm_start;
+ $asm_end = $asm_start + $match - 1;
+ $asm_start += $match;
+ $asm_start += $insert if($insert);
+ } else {
+ $asm_begin = $asm_start - $match + 1;
+ $asm_end = $asm_start;
+ $asm_start -= $match;
+ $asm_start -= $insert if($insert);
+ }
+ if($cmp_strand == 1) {
+ $cmp_begin = $cmp_start;
+ $cmp_end = $cmp_start + $match - 1;
+ $cmp_start += $match;
+ $cmp_start += $delete if($delete);
+ } else {
+ $cmp_begin = $cmp_start - $match + 1;
+ $cmp_end = $cmp_start;
+ $cmp_start -= $match;
+ $cmp_start -= $delete if($delete);
+ }
+
+ print join("\t",
+ $asm_sr_id, $cmp_sr_id,
+ $asm_begin, $asm_end,
+ $cmp_begin, $cmp_end,
+ $ori, "\n");
+
+ } else {
+ #empty line, next line is header line
+ $is_header_line = 1;
+ }
+ }
+}
+
+close(CHAIN_IN);
+
+sub usage {
+ print "\nusage: perl chain2assembly.pl -seq_region_in <filename> -chain_in <filename>\n";
+ print "\n seq_region_in must be a tab delimited text file dumped from an ensembl core".
+ "\n database (with revision > 19) that contains all of the seq_region names and ".
+ "\n identifiers used in the chain_in file.\n" .
+ "\n chain_in must be a chain format alignment file.\n" .
+ "\n assembly table data in tab delimited text format is printed to STDOUT\n";
+ exit 0;
+}
diff --git a/misc-scripts/qtl/extract_rat_map_ids.pl b/misc-scripts/qtl/extract_rat_map_ids.pl
new file mode 100644
index 0000000000..a9d90f8f09
--- /dev/null
+++ b/misc-scripts/qtl/extract_rat_map_ids.pl
@@ -0,0 +1,64 @@
+use strict;
+use warnings;
+use IO::File;
+use DBI;
+
+my $input = 'QTL2.txt';
+my $host = '127.0.0.1';
+my $dbname = 'rattus_norvegicus_core_13_2';
+my $port = '3308';
+my $user = 'ensro';
+my $pass = '';
+
+my $db = DBI->connect("DBI:mysql:host=$host;dbname=$dbname;port=$port;", $user, $pass, {'RaiseError' => 1});
+
+my $q = qq(
+SELECT source_primary_id, qtl_id FROM qtl WHERE source_database = 'rat genome database'
+);
+my $sth = $db->prepare($q);
+$sth->execute();
+
+my %rgd_hash = map {@$_} @{$sth->fetchall_arrayref};
+
+$sth->finish();
+$db->disconnect();
+
+my $fh = new IO::File;
+$fh->open($input);
+
+<$fh>; #throw away heading row
+my %ratmap_hash;
+while(<$fh>) {
+ chomp;
+ my @a = split(/\t/, $_);
+ my ($rgd_id, $ratmap_id) = ($a[4], $a[3]);
+
+ next if(!$ratmap_id || !$rgd_id);
+
+ if(exists($ratmap_hash{$ratmap_id})) {
+ warn "DUPLICATE identifier $ratmap_id\n";
+ next;
+ }
+
+ $ratmap_hash{$ratmap_id} = 1;
+
+ if(!exists($rgd_hash{$rgd_id})) {
+ warn "QTL RGD:$rgd_id not in database\n";
+ next;
+ }
+
+ my $ens_id = $rgd_hash{$rgd_id};
+ if($ens_id) {
+ print "INSERT INTO qtl_synonym (qtl_id, source_database, source_primary_id) VALUES ($ens_id, 'ratmap', $ratmap_id);\n";
+ } else {
+ #print "#RatMap QTL $ratmap_id not found\n";
+ }
+}
+
+$fh->close();
+
+foreach my $rgd_id (keys %rgd_hash) {
+ my $ens_id = $rgd_hash{$rgd_id};
+ print "INSERT INTO qtl_synonym (qtl_id, source_database, source_primary_id) VALUES ($ens_id, 'rat genome database', $rgd_id);\n";
+}
+
diff --git a/misc-scripts/surgery/SeqStoreConverter/AnophelesGambiae.pm b/misc-scripts/surgery/SeqStoreConverter/AnophelesGambiae.pm
new file mode 100644
index 0000000000..f2c6b63048
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/AnophelesGambiae.pm
@@ -0,0 +1,263 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::AnophelesGambiae;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("AnophelesGambiae Specific: creating scaffold, chunk and, " .
+ "chromosome coord systems");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (['chunk', undef, 'default_version,sequence_level'],
+ ['chromosome', $ass_def, 'top_level,default_version'],
+ ["scaffold" , undef, "default_version"]);
+
+ my @assembly_mappings = ("chromosome:$ass_def|chunk",
+ "chromosome:$ass_def|scaffold");
+
+ my %cs = (gene => 'chromosome',
+ transcript => 'chromosome',
+ exon => 'chromosome',
+ dna_align_feature => 'chunk',
+ protein_align_feature => 'chunk',
+ marker_feature => 'chunk',
+ simple_feature => 'chunk',
+ repeat_feature => 'chunk',
+ qtl_feature => 'chromosome',
+ misc_feature => 'chromosome',
+ prediction_transcript => 'chromosome',
+ karyotype => 'chromosome');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("AnophelesGambiae Specific: creating seq_regions");
+
+ $self->contig_to_seq_region('chunk');
+ $self->supercontig_to_seq_region('scaffold');
+ $self->chromosome_to_seq_region();
+}
+
+
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("AnophelesGambiae Specific: loading assembly table");
+
+ $self->assembly_contig_chromosome();
+ $self->assembly_supercontig_chromosome();
+
+ return;
+}
+
+
+sub transfer_prediction_transcripts {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $self->debug("AnophelesGambiae Specific: building prediction_exon table");
+
+ #
+ # In Anopheles the predicion transcripts were computed in chromosomal
+ # coords, so convert them to chromosomal coords and merge any adjacent
+ # exons
+ #
+
+ my $sql =
+ "SELECT pt.prediction_transcript_id, tcm.new_id as seq_region_id, " .
+ " IF(a.contig_ori=1,(pt.contig_start+a.chr_start-a.contig_start),".
+ " (a.chr_start+a.contig_end-pt.contig_end)) as start, " .
+ " IF(a.contig_ori=1,(pt.contig_end+a.chr_start-a.contig_start)," .
+ " (a.chr_start+a.contig_end-pt.contig_start)) as end, " .
+ " a.contig_ori * pt.contig_strand as strand, " .
+ " pt.start_phase, pt.score, pt.p_value " .
+ "FROM $source.assembly a, $target.tmp_chr_map tcm, " .
+ " $source.prediction_transcript pt " .
+ "WHERE pt.contig_id = a.contig_id " .
+ "AND a.chromosome_id = tcm.old_id " .
+ "ORDER BY pt.prediction_transcript_id, exon_rank";
+
+ my $sth = $dbh->prepare($sql);
+ $sth->execute();
+
+ my $prev_end = undef;
+ my $prev_start = undef;
+ my $prev_id = undef;
+ my $rank = 0;
+
+ my %prev_exon = ();
+
+ while(my $row = $sth->fetchrow_arrayref()) {
+ my ($pt_id, $sr_id, $sr_start, $sr_end, $sr_strand, $start_phase,
+ $score, $p_value) = @$row;
+
+ if(defined($prev_id) && ($prev_id == $pt_id)) {
+ #still in the same transcript
+
+ if($sr_strand == 1 &&
+ defined($prev_end) && $prev_end == $sr_start-1) {
+ $self->debug("merged exon $rank in prediction_transcript $pt_id\n");
+ #adjacent exons forward strand - merge them
+ $prev_exon{'seq_region_end'} = $sr_end;
+ $prev_end = $sr_end;
+ } elsif($sr_strand == -1 &&
+ defined($prev_start) && $prev_start == $sr_end+1) {
+ $self->debug("merged exon $rank in prediction_transcript $pt_id\n");
+ #adjacent exons negative strand - merge them
+ $prev_exon{'seq_region_start'} = $sr_start;
+ $prev_start = $sr_start;
+ } else {
+ #non-adjacent exons in the same transcript - no merge
+ $rank++;
+
+ #store the previous exon
+ $self->store_pexon(\%prev_exon);
+
+ #make current exon the previous exon
+ %prev_exon = ('prediction_transcript_id' => $pt_id,
+ 'seq_region_id' => $sr_id,
+ 'seq_region_start' => $sr_start,
+ 'seq_region_end' => $sr_end,
+ 'seq_region_strand' => $sr_strand,
+ 'start_phase' => $start_phase,
+ 'score' => $score,
+ 'p_value' => $p_value,
+ 'rank' => $rank);
+ }
+ } else {
+ #store previous exon
+ $self->store_pexon(\%prev_exon) if(%prev_exon);
+
+ #new ptranscript
+ $rank = 0;
+ $prev_id = $pt_id;
+ $prev_end = $sr_end;
+ $prev_start = $sr_start;
+ %prev_exon = ('prediction_transcript_id' => $pt_id,
+ 'seq_region_id' => $sr_id,
+ 'seq_region_start' => $sr_start,
+ 'seq_region_end' => $sr_end,
+ 'seq_region_strand' => $sr_strand,
+ 'start_phase' => $start_phase,
+ 'score' => $score,
+ 'p_value' => $p_value,
+ 'rank' => $rank);
+ }
+ }
+
+ #store the very last exon in the table
+ $self->store_pexon(\%prev_exon) if(%prev_exon);
+
+ $sth->finish();
+
+
+ $self->debug("AnophelesGambiae Specific: building prediction_transcript " .
+ "table");
+
+ $dbh->do
+ ("INSERT INTO $target.prediction_transcript (prediction_transcript_id, " .
+ " seq_region_id, seq_region_start, seq_region_end, " .
+ " seq_region_strand, analysis_id ) " .
+ "SELECT pt.prediction_transcript_id, tcm.new_id as seq_region_id, " .
+ " MIN(IF(a.contig_ori=1,(pt.contig_start+a.chr_start-a.contig_start),".
+ " (a.chr_start+a.contig_end-pt.contig_end))) as start, " .
+ " MAX(IF(a.contig_ori=1,(pt.contig_end+a.chr_start-a.contig_start)," .
+ " (a.chr_start+a.contig_end-pt.contig_start))) as end, " .
+ " a.contig_ori * pt.contig_strand as strand, " .
+ " pt.analysis_id " .
+ "FROM $source.assembly a, $target.tmp_chr_map tcm, " .
+ " $source.prediction_transcript pt " .
+ "WHERE pt.contig_id = a.contig_id " .
+ "AND a.chromosome_id = tcm.old_id " .
+ "GROUP BY prediction_transcript_id");
+
+ return;
+}
+
+
+#
+# helper function to store prediction exon
+#
+sub store_pexon {
+ my $self = shift;
+
+ my $pexon = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ my $store_sth = $dbh->prepare
+ ("INSERT INTO $target.prediction_exon (prediction_transcript_id, " .
+ " exon_rank, seq_region_id, seq_region_start, seq_region_end, " .
+ " seq_region_strand, start_phase, score, p_value) " .
+ "VALUES (?,?,?,?,?,?,?,?,?)");
+
+ $store_sth->execute($pexon->{'prediction_transcript_id'},
+ $pexon->{'rank'},
+ $pexon->{'seq_region_id'},
+ $pexon->{'seq_region_start'},
+ $pexon->{'seq_region_end'},
+ $pexon->{'seq_region_strand'},
+ $pexon->{'start_phase'},
+ $pexon->{'score'},
+ $pexon->{'p_value'});
+ $store_sth->finish();
+
+ return;
+}
+
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/BasicConverter.pm b/misc-scripts/surgery/SeqStoreConverter/BasicConverter.pm
new file mode 100644
index 0000000000..aec52c97b6
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/BasicConverter.pm
@@ -0,0 +1,1035 @@
+# Convert release 18-era schemas to use new non-clone/contig schema
+
+use strict;
+use warnings;
+use DBI;
+
+package SeqStoreConverter::BasicConverter;
+
+###############################################################################
+# Constructor
+###############################################################################
+
+sub new {
+ my ( $class, $user, $pass, $host, $source, $target, $schema, $force,
+ $verbose, $limit ) = @_;
+
+ my $self = bless {}, $class;
+
+ my $port;
+ ($host, $port) = split(/:/, $host);
+ $port ||= 3306;
+
+ my $dbh = DBI->connect( "DBI:mysql:host=$host:port=$port", $user, $pass,
+ {'RaiseError' => 1});
+
+
+ $self->verbose( $verbose );
+ $self->dbh( $dbh );
+ $self->force( $force );
+ $self->source( $source );
+ $self->target( $target );
+ $self->schema( $schema );
+ $self->host( $host );
+ $self->password( $pass);
+ $self->user($user);
+ $self->limit($limit);
+
+
+ #check to see if the destination and source databases exist already.
+ my %dbs = map {$_->[0] => 1} @{$dbh->selectall_arrayref('show databases')};
+ if( !$dbs{$source} ) {
+ die ("Source db $source does not exist" );
+ }
+
+ if( $dbs{$target} ) {
+ if( $force ) {
+ $dbh->do( "drop database $target" );
+ } else {
+ die("Target db $target already exists. Use -force option to overwrite.");
+ }
+ }
+
+ $dbh->do( "create database ".$self->target() );
+
+ $self->debug("Building schema for $target from $schema");
+ die "Cannot open $schema" if (! -e $schema);
+ my $cmd = "mysql -u $user -p$pass -h $host $target < $schema";
+ system ($cmd);
+
+
+ $self->debug("Creating temporary tables");
+ #create a temporary table to store the mapping of old ids to new ids
+ $dbh->do
+ ("CREATE TEMPORARY TABLE $target.tmp_cln_map (" .
+ "old_id INT, new_id INT, INDEX new_idx (new_id))");
+
+ #create a temp table which will store the mapping of old chromosome
+ #identifiers to new identifiers
+ $dbh->do("CREATE TEMPORARY TABLE $target.tmp_chr_map (" .
+ " old_id INT, new_id INT,".
+ " INDEX new_idx (new_id))");
+
+ #create a temporary table to hold old supercontig name -> new id mappings
+ $dbh->do("CREATE TEMPORARY TABLE $target.tmp_superctg_map (" .
+ "name VARCHAR(255), new_id INT, ".
+ "INDEX new_idx (new_id))");
+
+ return $self;
+}
+
+
+###############################################################################
+# Getter/Setters for converter properties
+###############################################################################
+
+sub force {
+ my $self = shift;
+ $self->{'force'} = shift if(@_);
+ return $self->{'force'};
+}
+
+
+sub dbh {
+ my $self = shift;
+ $self->{'dbh'} = shift if(@_);
+ return $self->{'dbh'};
+}
+
+sub user {
+ my $self = shift;
+ $self->{'user'} = shift if(@_);
+ return $self->{'user'};
+}
+
+sub host {
+ my $self = shift;
+ $self->{'host'} = shift if(@_);
+ return $self->{'host'};
+}
+
+sub password {
+ my $self = shift;
+ $self->{'password'} = shift if(@_);
+ return $self->{'password'};
+}
+
+
+sub verbose {
+ my $self = shift;
+ $self->{'verbose'} = shift if (@_);
+ return $self->{'verbose'};
+}
+
+sub schema {
+ my $self = shift;
+ $self->{'schema'} = shift if (@_);
+ return $self->{'schema'};
+}
+
+
+sub source {
+ my $self = shift;
+ $self->{'source'} = shift if(@_);
+ return $self->{'source'};
+}
+
+
+sub target {
+ my $self = shift;
+ $self->{'target'} = shift if(@_);
+ return $self->{'target'};
+}
+
+sub limit {
+ my $self = shift;
+ $self->{'limit'} = shift if(@_);
+ return $self->{'limit'};
+}
+
+
+###############################################################################
+# Utility methods
+###############################################################################
+
+sub debug {
+ my $self = shift;
+ my $str = shift;
+ print STDERR $str . "\n" if $self->verbose();
+ return;
+}
+
+sub copy_tables {
+ my ($self, @tables) = @_;
+
+ foreach my $table (@tables) {
+ $self->debug("Copying $table");
+
+ my $source = $self->source();
+ my $target = $self->target();
+
+ eval {
+ my $sth = $self->dbh()->prepare
+ ("INSERT INTO $target.$table SELECT * FROM $source.$table");
+ $sth->execute();
+ $sth->finish();
+ };
+
+ if($@) {
+ warn("Copy of table $table failed: $@\n");
+ }
+ }
+
+ return;
+}
+
+sub get_coord_system_id {
+ my $self = shift;
+ my $cs_name = shift;
+ my $cs_version = shift;
+
+ my $target = $self->target();
+
+ my @bind_vals = ($cs_name);
+ my $sql = "SELECT cs.coord_system_id " .
+ "FROM $target.coord_system cs " .
+ "WHERE cs.name = ?";
+
+ if($cs_version) {
+ push(@bind_vals, $cs_version);
+ $sql .= " AND cs.version = ?";
+ }
+
+ my $sth = $self->dbh()->prepare($sql);
+ $sth->execute(@bind_vals);
+
+ if($sth->rows() != 1) {
+ die("Id for non-existant or ambiguous coord system requested " .
+ "$cs_name:$cs_version");
+ }
+
+ my ($id) = $sth->fetchrow_array();
+
+ $sth->finish();
+
+ return $id;
+}
+
+sub get_default_assembly {
+ my $self = shift;
+
+ my $source = $self->source();
+
+ my $sth = $self->dbh->prepare
+ ("SELECT meta_value FROM $source.meta WHERE meta_key='assembly.default'");
+ $sth->execute();
+
+ if(!$sth->rows() == 1) {
+ die("This species has an ambiguous or non-existant assembly.default" .
+ " in the meta table");
+ }
+
+ my ($result) = $sth->fetchrow_array();
+
+ return $result;
+}
+
+
+
+sub contig_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= 'contig';
+
+ $self->debug("Transforming contigs into $target_cs_name seq_regions");
+
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ my $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT contig_id, name, $cs_id, length FROM $source.contig");
+
+ $sth->execute();
+ $sth->finish();
+
+ return;
+}
+
+
+sub clone_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ # target coord_system will have a different ID
+ $target_cs_name ||= "clone";
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ $self->debug("Transforming clones into $target_cs_name seq_regions");
+
+
+
+ my $select_sth = $dbh->prepare
+ ("SELECT cl.clone_id,
+ CONCAT(cl.embl_acc, '.', cl.embl_version),
+ MAX(ctg.embl_offset)+ctg.length-1
+ FROM $source.clone cl, $source.contig ctg
+ WHERE cl.clone_id = ctg.clone_id GROUP BY ctg.clone_id");
+ $select_sth->execute();
+
+ my ($clone_id, $embl_acc, $length);
+ $select_sth->bind_columns(\$clone_id, \$embl_acc, \$length);
+
+ my $insert_sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region (name, coord_system_id, length) " .
+ "VALUES(?,?,?)");
+
+ my $tmp_insert_sth = $dbh->prepare
+ ("INSERT INTO $target.tmp_cln_map (old_id, new_id) VALUES (?, ?)");
+
+ while ($select_sth->fetch()) {
+ $insert_sth->execute("$embl_acc", $cs_id, $length);
+
+ #store mapping of old -> new ids in temp table
+ $tmp_insert_sth->execute($clone_id, $insert_sth->{'mysql_insertid'});
+ }
+
+ $select_sth->finish();
+ $insert_sth->finish();
+ $tmp_insert_sth->finish();
+
+ return;
+}
+
+
+
+sub chromosome_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= "chromosome";
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ $self->debug("Transforming chromosomes into $target_cs_name seq_regions");
+
+
+ my $select_sth = $dbh->prepare
+ ("SELECT chromosome_id, name, length FROM $source.chromosome");
+
+ my $insert_sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region (name, coord_system_id, length) " .
+ "VALUES (?,?,?)");
+
+ my $tmp_insert_sth = $dbh->prepare
+ ("INSERT INTO $target.tmp_chr_map (old_id, new_id) VALUES (?, ?)");
+
+ $select_sth->execute();
+
+ my ($chrom_id, $name, $length);
+ $select_sth->bind_columns(\$chrom_id, \$name, \$length);
+
+ while ($select_sth->fetch()) {
+ #insert into seq_region table
+ $insert_sth->execute($name, $cs_id, $length);
+ #copy old/new mapping into temporary table
+ $tmp_insert_sth->execute($chrom_id, $insert_sth->{'mysql_insertid'});
+ }
+
+ $select_sth->finish();
+ $insert_sth->finish();
+ $tmp_insert_sth->finish();
+
+ return;
+}
+
+
+
+sub supercontig_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift || "supercontig";
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $self->debug("Transforming supercontigs into $target_cs_name seq_regions");
+
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ my $select_sth = $dbh->prepare
+ ("SELECT superctg_name, " .
+ "MAX(superctg_end)-MIN(superctg_start)+1 AS length " .
+ "FROM $source.assembly " .
+ "GROUP BY superctg_name");
+
+ my $insert_sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region (name, coord_system_id, length) " .
+ "VALUES (?,?,?)");
+
+ my $tmp_insert_sth = $dbh->prepare
+ ("INSERT INTO $target.tmp_superctg_map (name, new_id) VALUES (?, ?)");
+
+ my ($name, $length);
+ $select_sth->execute();
+ $select_sth->bind_columns(\$name, \$length);
+
+ while ($select_sth->fetch()) {
+ $insert_sth->execute($name, $cs_id, $length);
+ $tmp_insert_sth->execute($name, $insert_sth->{'mysql_insertid'});
+ }
+
+ $select_sth->finish();
+ $insert_sth->finish();
+ $tmp_insert_sth->finish();
+
+ return;
+}
+
+
+sub assembly_contig_chromosome {
+ my $self = shift;
+
+ $self->debug("Building assembly table - contig/chromosome");
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $dbh->do(
+ "INSERT INTO $target.assembly " .
+ "SELECT tcm.new_id, " . # asm_seq_region_id (old-new chromosome ID mapping)
+ "a.contig_id, " . # cmp_seq_region_id
+ "a.chr_start, " . # asm_start
+ "a.chr_end, " . # asm_end
+ "a.contig_start, " . # cmp_start
+ "a.contig_end, " . # cmp_end
+ "a.contig_ori " . # ori
+ "FROM $target.tmp_chr_map tcm, $source.assembly a, $source.contig c " .
+ "WHERE tcm.old_id = a.chromosome_id " .
+ "AND c.contig_id = a.contig_id "); # only copy assembly entries that
+ # refer to valid contigs (test db has
+ # superfluous assembly entries)
+
+}
+
+sub assembly_contig_clone {
+ my $self = shift;
+
+ $self->debug("Building assembly table - contig/clone");
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $dbh->do(
+ "INSERT INTO $target.assembly " .
+ "SELECT tcm.new_id, " . # asm_seq_region_id (old-new clone ID mapping)
+ "ctg.contig_id, ". # cmp_seq_region_id
+ "ctg.embl_offset, " . # asm_start
+ "ctg.embl_offset+ctg.length-1, " . # asm_end
+ "1, " . # cmp_start
+ "ctg.length, " . # cmp_end
+ "1 " . # ori - contig always positively oriented on the clone
+ "FROM $target.tmp_cln_map tcm, " .
+ "$source.clone cln, $source.contig ctg " .
+ "WHERE tcm.old_id = cln.clone_id " .
+ "AND cln.clone_id = ctg.clone_id");
+
+}
+
+
+sub assembly_contig_supercontig {
+ my $self = shift;
+
+ $self->debug("Building assembly table - contig/supercontig");
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $dbh->do(
+ "INSERT INTO $target.assembly " .
+ "SELECT tsm.new_id, " . # asm_seq_region_id (superctg name-sr_id mapping)
+ "a.contig_id, " . # cmp_seq_region_id
+ "a.superctg_start, " . # asm_start
+ "a.superctg_end, " . # asm_end
+ "a.contig_start, " . # cmp_start
+ "a.contig_end, " . # cmp_end
+ "a.contig_ori " . # ori
+ "FROM $target.tmp_superctg_map tsm, $source.assembly a, $source.contig c ".
+ "WHERE tsm.name = a.superctg_name " .
+ "AND c.contig_id = a.contig_id "); # only copy assembly entries that
+ # refer to valid contigs (test db might
+ # have these)
+
+}
+
+
+
+sub assembly_supercontig_chromosome {
+ my $self = shift;
+
+ $self->debug("Building assembly table - supercontig/chromosome");
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $dbh->do(
+ "INSERT INTO $target.assembly " .
+ "SELECT tcm.new_id, " . # asm_seq_region_id (chr id)
+ "tsm.new_id, " . # cmp_seq_region_id (supercontig id)
+ "min(a.chr_start), " . # asm_start
+ "max(a.chr_end), " . # asm_end
+ "min(a.superctg_start), " . # cmp_start
+ "max(a.superctg_end), " . # cmp_end
+ "a.superctg_ori " . # ori
+ "FROM $target.tmp_superctg_map tsm, $target.tmp_chr_map tcm, " .
+ " $source.assembly a ".
+ "WHERE tsm.name = a.superctg_name " .
+ "AND tcm.old_id = a.chromosome_id " .
+ "GROUP BY superctg_name");
+
+}
+
+
+
+###############################################################################
+# Base class implementations of transfer methods. Can be overridden to
+# create species specific behaviour
+###############################################################################
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["chromosome" , $ass_def, "top_level,default_version" ],
+ ["supercontig", undef , "default_version" ],
+ ["clone" , undef , "default_version" ],
+ ["contig", undef , "default_version,sequence_level"]);
+
+ my @assembly_mappings = ("chromosome:$ass_def|contig",
+ "clone|contig",
+ "supercontig|contig");
+
+ my %cs = (gene => 'chromosome',
+ transcript => 'chromosome',
+ exon => 'chromosome',
+ dna_align_feature => 'contig',
+ protein_align_feature => 'contig',
+ marker_feature => 'contig',
+ simple_feature => 'contig',
+ repeat_feature => 'contig',
+ qtl_feature => 'chromosome',
+ misc_feature => 'chromosome',
+ prediction_transcript => 'contig',
+ karyotype => 'chromosome');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $self->debug("Adding assembly.mapping entries to meta table");
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ #default behaviour is to simply copy all tables as they come
+
+ $self->contig_to_seq_region('contig');
+ $self->chromosome_to_seq_region();
+ $self->supercontig_to_seq_region();
+ $self->clone_to_seq_region();
+
+ return;
+}
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->assembly_contig_chromosome();
+ $self->assembly_contig_clone();
+ $self->assembly_contig_supercontig();
+
+ return;
+}
+
+sub transfer_dna {
+ my $self = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $self->debug("Building dna table");
+
+ $dbh->do("INSERT INTO $target.dna " .
+ "SELECT c.contig_id as seq_region_id, d.sequence as sequence " .
+ "FROM $source.dna d, $source.contig c " .
+ "WHERE c.dna_id = d.dna_id");
+ return;
+}
+
+
+sub transfer_genes {
+ my $self = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ #
+ # Transfer the gene table
+ #
+
+ $self->debug("Building gene table");
+
+ $dbh->do
+ ("INSERT INTO $target.gene " .
+ "SELECT g.gene_id, g.type, g.analysis_id, tcm.new_id, " .
+ "MIN(IF (a.contig_ori=1,(e.contig_start+a.chr_start-a.contig_start)," .
+ " (a.chr_start+a.contig_end-e.contig_end ))) as start, " .
+ "MAX(IF (a.contig_ori=1,(e.contig_end+a.chr_start-a.contig_start), " .
+ " (a.chr_start+a.contig_end-e.contig_start))) as end, " .
+ " a.contig_ori*e.contig_strand as strand, " .
+ " g.display_xref_id " .
+ "FROM $source.transcript t, $source.exon_transcript et, " .
+ " $source.exon e, $source.assembly a, $source.gene g, " .
+ " $target.tmp_chr_map tcm " .
+ "WHERE t.transcript_id = et.transcript_id " .
+ "AND et.exon_id = e.exon_id " .
+ "AND e.contig_id = a.contig_id " .
+ "AND g.gene_id = t.gene_id " .
+ "AND a.chromosome_id = tcm.old_id " .
+ "GROUP BY g.gene_id");
+
+
+ #
+ # Transfer the transcript table
+ #
+ $self->debug("Building transcript table");
+
+ $dbh->do
+ ("INSERT INTO $target.transcript " .
+ "SELECT t.transcript_id, t.gene_id, tcm.new_id, " .
+ "MIN(IF (a.contig_ori=1,(e.contig_start+a.chr_start-a.contig_start)," .
+ " (a.chr_start+a.contig_end-e.contig_end ))) as start, " .
+ "MAX(IF (a.contig_ori=1,(e.contig_end+a.chr_start-a.contig_start), " .
+ " (a.chr_start+a.contig_end-e.contig_start))) as end, " .
+ " a.contig_ori*e.contig_strand as strand, " .
+ " t.display_xref_id " .
+ "FROM $source.transcript t, $source.exon_transcript et, " .
+ " $source.exon e, $source.assembly a, $target.tmp_chr_map tcm " .
+ "WHERE t.transcript_id = et.transcript_id " .
+ "AND et.exon_id = e.exon_id " .
+ "AND e.contig_id = a.contig_id " .
+ "AND a.chromosome_id = tcm.old_id " .
+ "GROUP BY t.transcript_id");
+
+ #
+ # Transfer the exon table
+ #
+ $self->debug("Building exon table");
+
+ $dbh->do
+ ("INSERT INTO $target.exon " .
+ "SELECT e.exon_id, tcm.new_id, " .
+ "MIN(IF (a.contig_ori=1,(e.contig_start+a.chr_start-a.contig_start)," .
+ " (a.chr_start+a.contig_end-e.contig_end ))) as start, " .
+ "MAX(IF (a.contig_ori=1,(e.contig_end+a.chr_start-a.contig_start), " .
+ " (a.chr_start+a.contig_end-e.contig_start))) as end, " .
+ " a.contig_ori*e.contig_strand as strand, " .
+ " e.phase, e.end_phase " .
+ "FROM $source.transcript t, $source.exon_transcript et, " .
+ " $source.exon e, $source.assembly a, $source.gene g, " .
+ " $target.tmp_chr_map tcm " .
+ "WHERE t.transcript_id = et.transcript_id " .
+ "AND et.exon_id = e.exon_id " .
+ "AND e.contig_id = a.contig_id " .
+ "AND g.gene_id = t.gene_id " .
+ "AND a.chromosome_id = tcm.old_id " .
+ "GROUP BY e.exon_id");
+
+ #
+ # Transfer translation table
+ #
+
+ $self->debug("Building translation table");
+
+ $dbh->do
+ ("INSERT INTO $target.translation " .
+ "SELECT tl.translation_id, ts.transcript_id, tl.seq_start, " .
+ " tl.start_exon_id, tl.seq_end, tl.end_exon_id " .
+ "FROM $source.transcript ts, $source.translation tl " .
+ "WHERE ts.translation_id = tl.translation_id");
+
+ return;
+}
+
+sub transfer_prediction_transcripts {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ # prediction_transcript / prediction_exon
+
+ $self->debug( "Building prediction_exon table" );
+
+ $dbh->do
+ ("INSERT INTO $target.prediction_exon ".
+ "( prediction_transcript_id, seq_region_id, seq_region_start, " .
+ " seq_region_end, seq_region_strand, start_phase, score, p_value," .
+ " exon_rank ) " .
+ "SELECT prediction_transcript_id, contig_id, contig_start, contig_end, " .
+ " contig_strand, start_phase, score, p_value, exon_rank " .
+ "FROM $source.prediction_transcript" );
+
+ $self->debug("Building prediction_transcript table");
+
+ $dbh->do
+ ("INSERT INTO $target.prediction_transcript ".
+ "( prediction_transcript_id, seq_region_id, seq_region_start, " .
+ "seq_region_end, seq_region_strand, analysis_id ) " .
+ "SELECT prediction_transcript_id, contig_id, MIN(contig_start), " .
+ " MAX(contig_end), contig_strand, analysis_id ".
+ "FROM $source.prediction_transcript " .
+ "GROUP BY prediction_transcript_id ");
+
+ return;
+}
+
+
+
+sub transfer_features {
+ my $self = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ my $limit = '';
+ if($self->limit()) {
+ $limit = ' limit ' . $self->limit();
+ }
+
+
+ #
+ # Feature tables
+ # Note that we can just rename contig_* to set_region_* since the
+ # contig IDs were copied verbatim into seq_region
+ #
+
+ # For some reason mysql occasionally refuses to use the index on large
+ # tables following copies like the following.
+ # So: drop the indexes first and then re-add them after
+
+ # simple_feature
+ $self->debug("Building simple_feature table");
+ $dbh->do
+ ("INSERT INTO $target.simple_feature (simple_feature_id, seq_region_id, ".
+ " seq_region_start, seq_region_end, seq_region_strand, " .
+ " display_label, analysis_id, score) " .
+ "SELECT simple_feature_id, contig_id, contig_start, contig_end, " .
+ " contig_strand, display_label, analysis_id, score " .
+ "FROM $source.simple_feature $limit");
+
+ # repeat_feature
+ $self->debug("Dropping indexes on repeat_feature");
+ $dbh->do("ALTER TABLE $target.repeat_feature DROP INDEX seq_region_idx");
+ $dbh->do("ALTER TABLE $target.repeat_feature DROP INDEX repeat_idx");
+ $dbh->do("ALTER TABLE $target.repeat_feature DROP INDEX analysis_idx");
+
+ $self->debug("Building repeat_feature table");
+ $dbh->do
+ ("INSERT INTO $target.repeat_feature (repeat_feature_id, seq_region_id, " .
+ " seq_region_start, seq_region_end, seq_region_strand, analysis_id, " .
+ " repeat_start, repeat_end, repeat_consensus_id, score) " .
+ "SELECT repeat_feature_id, contig_id, contig_start, contig_end, " .
+ " contig_strand, analysis_id, repeat_start, repeat_end, " .
+ " repeat_consensus_id, score FROM $source.repeat_feature $limit");
+
+ $self->debug("Readding indexes on repeat_feature");
+ $dbh->do("ALTER TABLE $target.repeat_feature " .
+ "ADD INDEX seq_region_idx( seq_region_id, seq_region_start)");
+ $dbh->do("ALTER TABLE $target.repeat_feature " .
+ "ADD INDEX repeat_idx( repeat_consensus_id )");
+ $dbh->do("ALTER TABLE $target.repeat_feature " .
+ "ADD INDEX analysis_idx(analysis_id)");
+
+ # protein_align_feature
+ $self->debug("Dropping indexes on protein_align_feature");
+ $dbh->do("ALTER TABLE $target.protein_align_feature DROP INDEX hit_idx");
+ $dbh->do( "ALTER TABLE $target.protein_align_feature " .
+ "DROP INDEX seq_region_idx");
+
+ $self->debug("Building protein_align_feature table");
+ $dbh->do( "INSERT INTO $target.protein_align_feature " .
+ " (protein_align_feature_id, seq_region_id, seq_region_start, " .
+ " seq_region_end, seq_region_strand, analysis_id, hit_start, " .
+ " hit_end, hit_name, cigar_line, evalue, perc_ident, score) " .
+ "SELECT protein_align_feature_id, contig_id, contig_start, " .
+ " contig_end, contig_strand, analysis_id, hit_start, " .
+ " hit_end, hit_name, cigar_line, evalue, perc_ident, score ".
+ "FROM $source.protein_align_feature $limit");
+
+ $self->debug("Readding indexes on protein_align_feature");
+ $dbh->do( "ALTER TABLE $target.protein_align_feature " .
+ "ADD index seq_region_idx( seq_region_id, analysis_id, score,".
+ " seq_region_start )");
+ $dbh->do( "ALTER TABLE $target.protein_align_feature " .
+ "ADD index hit_idx(hit_name)");
+
+ # dna_align_feature
+ $self->debug("Dropping indexes on dna_align_feature");
+ $dbh->do( "ALTER TABLE $target.dna_align_feature DROP INDEX seq_region_idx");
+ $dbh->do( "ALTER TABLE $target.dna_align_feature DROP INDEX hit_idx");
+
+ $self->debug("Building dna_align_feature table");
+ $dbh->do( "INSERT INTO $target.dna_align_feature " .
+ " (dna_align_feature_id, seq_region_id, seq_region_start, ".
+ " seq_region_end, seq_region_strand, analysis_id, " .
+ " hit_start, hit_end, hit_name, hit_strand, cigar_line, " .
+ " evalue, perc_ident, score) " .
+ "SELECT dna_align_feature_id, contig_id, contig_start, " .
+ " contig_end, contig_strand, analysis_id, hit_start, " .
+ " hit_end, hit_name, hit_strand, cigar_line, evalue, " .
+ " perc_ident, score FROM $source.dna_align_feature $limit");
+
+
+ $self->debug("Readding indexes on dna_align_feature");
+ $dbh->do( "ALTER TABLE $target.dna_align_feature " .
+ "ADD INDEX seq_region_idx(seq_region_id, analysis_id, score, " .
+ " seq_region_start)");
+ $dbh->do( "ALTER TABLE $target.dna_align_feature " .
+ "ADD index hit_idx(hit_name)");
+
+ # marker_feature
+ $self->debug("Building marker_feature table");
+ $dbh->do( "INSERT INTO $target.marker_feature " .
+ " (marker_feature_id, marker_id, seq_region_id, " .
+ " seq_region_start, seq_region_end, analysis_id, " .
+ " map_weight) " .
+ "SELECT marker_feature_id, marker_id, contig_id, contig_start, ".
+ " contig_end, analysis_id, map_weight " .
+ "FROM $source.marker_feature $limit");
+
+ # qtl_feature
+ # Note this uses chromosome coords so we have to join with tmp_chr_map to
+ # get the mapping
+ $self->debug("Building qtl_feature table");
+
+ $dbh->do
+ ("INSERT INTO $target.qtl_feature( seq_region_id, seq_region_start, " .
+ " seq_region_end, qtl_id, analysis_id) " .
+ "SELECT tcm.new_id, " .
+ " q.start, q.end, q.qtl_id, q.analysis_id " .
+ "FROM $target.tmp_chr_map tcm, $source.qtl_feature q " .
+ "WHERE tcm.old_id = q.chromosome_id $limit");
+
+ # These tables now have seq_region_* instead of chromosome_*
+
+ $self->debug("Building karyotype table");
+ $dbh->do(
+ "INSERT INTO $target.karyotype " .
+ "SELECT null, tcm.new_id, " .
+ " k.chr_start, k.chr_end, k.band, k.stain " .
+ "FROM $target.tmp_chr_map tcm, $source.karyotype k " .
+ "WHERE tcm.old_id = k.chromosome_id $limit");
+
+
+ $self->debug("Building marker_map_location table");
+ $dbh->do(
+ "INSERT INTO $target.marker_map_location " .
+ "SELECT mml.marker_id, mml.map_id, " .
+ " c.name, " .
+ " mml.marker_synonym_id, mml.position, mml.lod_score " .
+ "FROM $source.chromosome c, $source.marker_map_location mml " .
+ "WHERE c.chromosome_id = mml.chromosome_id $limit");
+
+ $self->debug("Building map_density table");
+ $dbh->do(
+ "INSERT INTO $target.map_density " .
+ "SELECT tcm.new_id, ".
+ " md.chr_start, md.chr_end, md.type, md.value " .
+ "FROM $target.tmp_chr_map tcm, $source.map_density md " .
+ "WHERE tcm.old_id = md.chromosome_id $limit");
+
+
+ $self->debug( "Building misc_feature table" );
+ $dbh->do
+ ("INSERT INTO $target.misc_feature( misc_feature_id, seq_region_id, " .
+ " seq_region_start, seq_region_end, seq_region_strand ) " .
+ "SELECT m.mapfrag_id, sr.seq_region_id, m.seq_start, m.seq_end, " .
+ " m.orientation " .
+ "FROM $source.mapfrag m, $target.seq_region sr, $source.dnafrag d " .
+ "WHERE m.dnafrag_id = d.dnafrag_id " .
+ "AND d.name = sr.name $limit" );
+
+ $self->debug( "Building misc_set table" );
+ $dbh->do
+ ("INSERT INTO $target.misc_set( misc_set_id, code, name, description, " .
+ " max_length ) " .
+ "SELECT mapset_id, code, name, description, max_length " .
+ "FROM $source.mapset ms" );
+
+ $self->debug( "Building misc_attrib table" );
+ $dbh->do
+ ("INSERT INTO $target.misc_attrib( misc_feature_id, attrib_type_id, " .
+ " value ) ".
+ "SELECT mapfrag_id, mapannotationtype_id, value " .
+ "FROM $source.mapannotation" );
+
+ $self->debug( "Building misc_feature_misc_set table" );
+ $dbh->do
+ ("INSERT INTO $target.misc_feature_misc_set(misc_feature_id, misc_set_id)".
+ "SELECT mapfrag_id, mapset_id ".
+ "FROM $source.mapfrag_mapset $limit" );
+
+ return;
+}
+
+
+sub transfer_stable_ids {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $self->debug("Building stable id tables");
+
+ #
+ # Copy the stable id tables
+ # remove the unused created and modified dates from the stable ids
+ #
+ $dbh->do
+ ("INSERT INTO $target.exon_stable_id " .
+ " (exon_id, stable_id, version) " .
+ "SELECT exon_id, stable_id, version " .
+ "FROM $source.exon_stable_id" );
+
+ $dbh->do
+ ("INSERT INTO $target.gene_stable_id " .
+ " (gene_id, stable_id, version) " .
+ "SELECT gene_id, stable_id, version " .
+ "FROM $source.gene_stable_id" );
+
+ $self->copy_tables
+ ("stable_id_event","mapping_session","transcript_stable_id",
+ "translation_stable_id","gene_archive","peptide_archive");
+
+ return;
+}
+
+sub transfer_meta {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+
+ my $dbh = $self->dbh();
+
+ $dbh->do("INSERT INTO $target.meta (meta_key, meta_value) " .
+ "SELECT m.meta_key, m.meta_value FROM $source.meta m " .
+ "ORDER BY meta_id");
+
+ return;
+}
+
+
+sub copy_other_tables {
+ my $self = shift;
+
+ #xref tables
+ $self->copy_tables("xref",
+ "go_xref",
+ "identity_xref",
+ "object_xref",
+ "external_db",
+ "external_synonym",
+ #marker/qtl related tables
+ "map",
+ "marker",
+ "marker_synonym",
+ "qtl",
+ "qtl_synonym",
+ #misc other tables
+ "supporting_feature",
+ "analysis",
+ "exon_transcript",
+ "interpro",
+ "gene_description",
+ "protein_feature",
+ "repeat_consensus");
+}
+
+
+sub create_attribs {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ #copy the attrib types from the mapannotation type table
+
+ $dbh->do
+ ("INSERT INTO $target.attrib_type( attrib_type_id, code, " .
+ " name, description ) " .
+ "SELECT mapannotationtype_id, code, name, description " .
+ "FROM $source.mapannotationtype " );
+
+ return;
+}
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/CaenorhabditisBriggsae.pm b/misc-scripts/surgery/SeqStoreConverter/CaenorhabditisBriggsae.pm
new file mode 100644
index 0000000000..044f07d30a
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/CaenorhabditisBriggsae.pm
@@ -0,0 +1,254 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::CaenorhabditisBriggsae;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("CaenorhabditisBriggsae Specific: creating clone, scaffold," .
+ " and contig coordinate systems");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["scaffold" , $ass_def, "top_level,default_version" ],
+ ['clone' , undef , 'default_version' ],
+ ["contig" , undef , "default_version,sequence_level"]);
+
+ my @assembly_mappings = ("scaffold:$ass_def|contig",
+ "clone|contig");
+
+ my %cs = (gene => 'scaffold',
+ transcript => 'scaffold',
+ exon => 'scaffold',
+ dna_align_feature => 'contig',
+ protein_align_feature => 'contig',
+ marker_feature => 'contig',
+ simple_feature => 'contig',
+ repeat_feature => 'contig',
+ qtl_feature => 'scaffold',
+ misc_feature => 'scaffold',
+ prediction_transcript => 'contig',
+ karyotype => 'scaffold');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $self->debug("Adding assembly.mapping entries to meta table");
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("CaenorhabditisBriggsae Specific: creating contig, " .
+ "clone, contig and scaffold seq_regions");
+
+ $self->contig_to_seq_region();
+ $self->clone_to_seq_region();
+ $self->chromosome_to_seq_region('scaffold');
+}
+
+
+sub chromosome_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= "chromosome";
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ $self->debug("CaenorhabditisBriggsae Specific: Transforming " .
+ "chromosomes into $target_cs_name seq_regions");
+
+ #strip off the leading 'cb25.' from the chromosome name
+ my $select_sth = $dbh->prepare
+ ("SELECT chromosome_id,substring(name,6),length FROM $source.chromosome");
+
+ my $insert_sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region (name, coord_system_id, length) " .
+ "VALUES (?,?,?)");
+
+ my $tmp_insert_sth = $dbh->prepare
+ ("INSERT INTO $target.tmp_chr_map (old_id, new_id) VALUES (?, ?)");
+
+ $select_sth->execute();
+
+ my ($chrom_id, $name, $length);
+ $select_sth->bind_columns(\$chrom_id, \$name, \$length);
+
+ while ($select_sth->fetch()) {
+ #insert into seq_region table
+ $insert_sth->execute($name, $cs_id, $length);
+ #copy old/new mapping into temporary table
+ $tmp_insert_sth->execute($chrom_id, $insert_sth->{'mysql_insertid'});
+ }
+
+ $select_sth->finish();
+ $insert_sth->finish();
+ $tmp_insert_sth->finish();
+
+ return;
+}
+
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("CaenorhabditisBriggsae Specific: loading assembly data");
+
+ $self->assembly_contig_chromosome();
+ $self->assembly_contig_clone();
+}
+
+#
+# Override contig_to_seq_region and clone_to_seq_region to provide
+# briggsae specific behaviour
+#
+
+sub contig_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= 'contig';
+
+ $self->debug("Caenorhabditis Specific: Transforming contigs into " .
+ "$target_cs_name seq_regions");
+
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ #There are two types of contigs in briggsae:
+
+ #
+ # cosmids/clones
+ #
+ my $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT contig_id, name, $cs_id, length " .
+ "FROM $source.contig " .
+ "WHERE name not like 'c%'");
+
+ $sth->execute();
+ $sth->finish();
+
+ #
+ # WGS contigs
+ #
+ $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT ctg.contig_id, cln.name, $cs_id, length " .
+ "FROM $source.contig ctg, $source.clone cln " .
+ "WHERE ctg.clone_id = cln.clone_id " .
+ "AND ctg.name like 'c%'");
+
+ $sth->execute();
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub clone_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ # target coord_system will have a different ID
+ $target_cs_name ||= "clone";
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ $self->debug("Caenorhabditis Specific:Transforming clones " .
+ "into $target_cs_name seq_regions");
+
+ #
+ # We don't want to make clones out of the WGS contigs, only out of
+ # the clones with proper embl accessions
+ #
+ my $select_sth = $dbh->prepare
+ ("SELECT cl.clone_id,
+ CONCAT(cl.embl_acc, '.', cl.embl_version),
+ MAX(ctg.embl_offset)+ctg.length-1
+ FROM $source.clone cl, $source.contig ctg
+ WHERE cl.clone_id = ctg.clone_id
+ AND cl.embl_acc not like 'c%'
+ GROUP BY ctg.clone_id");
+
+ $select_sth->execute();
+
+ my ($clone_id, $embl_acc, $length);
+ $select_sth->bind_columns(\$clone_id, \$embl_acc, \$length);
+
+ my $insert_sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region (name, coord_system_id, length) " .
+ "VALUES(?,?,?)");
+
+ my $tmp_insert_sth = $dbh->prepare
+ ("INSERT INTO $target.tmp_cln_map (old_id, new_id) VALUES (?, ?)");
+
+ while ($select_sth->fetch()) {
+ $insert_sth->execute("$embl_acc", $cs_id, $length);
+
+ #store mapping of old -> new ids in temp table
+ $tmp_insert_sth->execute($clone_id, $insert_sth->{'mysql_insertid'});
+ }
+
+ $select_sth->finish();
+ $insert_sth->finish();
+ $tmp_insert_sth->finish();
+
+ return;
+}
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/CaenorhabditisElegans.pm b/misc-scripts/surgery/SeqStoreConverter/CaenorhabditisElegans.pm
new file mode 100644
index 0000000000..3a15e1241c
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/CaenorhabditisElegans.pm
@@ -0,0 +1,133 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::CaenorhabditisElegans;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("Caenorhabditis Specific: creating " .
+ "clone and chromosome coord systems");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["chromosome" , $ass_def, "top_level,default_version" ],
+ ["clone", undef , "default_version,sequence_level"]);
+
+ my @assembly_mappings = ("chromosome:$ass_def|clone");
+
+ my %cs = (gene => 'chromosome',
+ transcript => 'chromosome',
+ exon => 'chromosome',
+ dna_align_feature => 'clone',
+ protein_align_feature => 'clone',
+ marker_feature => 'clone',
+ simple_feature => 'clone',
+ repeat_feature => 'clone',
+ qtl_feature => 'chromosome',
+ misc_feature => 'chromosome',
+ prediction_transcript => 'clone',
+ karyotype => 'chromosome');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $self->debug("Adding assembly.mapping entries to meta table");
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("CaenorhabditisElegans Specific: creating clone and " .
+ "chromosome seq_regions");
+
+ $self->contig_to_seq_region('clone');
+ $self->chromosome_to_seq_region();
+}
+
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("CaenorhabditisElegans Specific: loading assembly data");
+
+ $self->assembly_contig_chromosome();
+}
+
+
+#
+# override the contig_to_seqregion method so that contigs are given clone
+# names instead
+#
+sub contig_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= 'contig';
+
+ $self->debug("CaenorhabditisElegans Specific: Transforming contigs " .
+ "into $target_cs_name seq_regions");
+
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ my $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT ctg.contig_id, CONCAT(cln.embl_acc, '.', cln.embl_version), " .
+ " $cs_id, ctg.length " .
+ "FROM $source.contig ctg, $source.clone cln " .
+ "WHERE ctg.clone_id = cln.clone_id");
+
+ $sth->execute();
+ $sth->finish();
+
+ return;
+}
+
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/DanioRerio.pm b/misc-scripts/surgery/SeqStoreConverter/DanioRerio.pm
new file mode 100644
index 0000000000..bc41c0abf5
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/DanioRerio.pm
@@ -0,0 +1,335 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::DanioRerio;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("DanioRerio Specific: creating scaffold coord system");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["scaffold" , $ass_def, "top_level,default_version,sequence_level"]);
+
+ my %cs = (gene => 'scaffold',
+ transcript => 'scaffold',
+ exon => 'scaffold',
+ dna_align_feature => 'scaffold',
+ protein_align_feature => 'scaffold',
+ marker_feature => 'scaffold',
+ simple_feature => 'scaffold',
+ repeat_feature => 'scaffold',
+ qtl_feature => 'scaffold',
+ misc_feature => 'scaffold',
+ prediction_transcript => 'scaffold',
+ karyotype => 'scaffold');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ return;
+}
+
+
+
+#
+# getter/setter needed by danio rerio to perform coord transforms
+# of features after conversion of fake contigs in scaffolds
+#
+sub contig_mappings {
+ my $self = shift;
+ $self->{'contig_mappings'} = shift if(@_);
+ return $self->{'contig_mappings'};
+}
+
+
+sub dna_mappings {
+ my $self = shift;
+ $self->{'dna_mappings'} = shift if(@_);
+ return $self->{'dna_mappings'};
+}
+
+sub create_seq_regions {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $self->debug("DanioRerio Specific: creating scaffold seq_regions");
+
+ #
+ # retrieve fake contigs and group them into scaffolds
+ #
+
+ my $sth = $dbh->prepare
+ ("SELECT c.contig_id, c.name, " .
+ "a.contig_end - a.contig_start+1, dna_id " .
+ "FROM $source.contig c, $source.assembly a " .
+ "WHERE c.contig_id = a.contig_id");
+ $sth->execute();
+
+ my %contig_lists;
+
+ my($contig_id, $name, $length, $dna_id);
+ $sth->bind_columns(\$contig_id, \$name, \$length, \$dna_id);
+
+ while($sth->fetch()) {
+ my $position;
+ ($name, $position) = split(/\./, $name);
+ $contig_lists{$name} ||= [];
+ $contig_lists{$name}->[$position-1] = [$contig_id,$length,$dna_id];
+ }
+
+ $sth->finish();
+
+ #
+ # calculate the offsets of the fake contigs, and the lengths of the
+ # scaffolds
+ #
+
+ my %old_new_mappings;
+ my %dna_mappings;
+
+ my %scaffolds;
+ foreach my $ctgname (keys %contig_lists) {
+ my $ctg_list = $contig_lists{$ctgname};
+ $length = 0;
+ my $new_id = $ctg_list->[0]->[0];
+ $dna_mappings{$new_id} = [];
+ foreach my $contig (@$ctg_list) {
+ my $old_id = $contig->[0];
+ $old_new_mappings{$old_id} = [$new_id,$length+1];
+ $length += $contig->[1];
+ push(@{$dna_mappings{$new_id}}, [$contig->[2], $contig->[1]]);
+ }
+
+ $scaffolds{$ctgname} = [$new_id, $length];
+ }
+
+
+ #
+ # load the scaffold seq regions
+ #
+ $self->debug("DanioRerio Specific: loading scaffold seq regions");
+
+ my $cs_id = $self->get_coord_system_id('scaffold');
+
+ $sth = $dbh->prepare("INSERT INTO $target.seq_region (seq_region_id, " .
+ "name, length, coord_system_id) " .
+ "VALUES (?,?,?,?)");
+
+ foreach my $scaf_name (keys %scaffolds) {
+ my ($seq_reg_id, $len) = @{$scaffolds{$scaf_name}};
+ $sth->execute($seq_reg_id, $scaf_name, $len, $cs_id);
+ }
+ $sth->finish();
+
+
+ #
+ # load temporary mapping of chromosomes to new seq_region ids. Even though
+ # there are not actually any chromosomes this info is used when transfering
+ # genes to scaffold coords.
+ #
+ my $chr_select_sth = $dbh->prepare
+ ("SELECT chromosome_id, name FROM $source.chromosome");
+ $chr_select_sth->execute();
+
+ my $tmp_insert_sth = $dbh->prepare
+ ("INSERT INTO $target.tmp_chr_map (old_id, new_id) VALUES (?,?)");
+
+ while(my $row = $chr_select_sth->fetchrow_arrayref) {
+ my ($chr_id, $chr_name) = @$row;
+ $tmp_insert_sth->execute($chr_id, $scaffolds{$chr_name}->[0]);
+ }
+
+ #keep this mapping info for later processing of features & dna
+ $self->dna_mappings(\%dna_mappings);
+ $self->contig_mappings(\%old_new_mappings);
+
+}
+
+
+
+sub transfer_dna {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $self->debug("DanioRerio Specific: building dna table");
+
+ #in danio rerio est/estgene databases there is no dna so we may want to
+ #skip this function
+
+ my $count_sth = $dbh->prepare("SELECT count(*) from $source.dna");
+ $count_sth->execute();
+ my ($count) = $count_sth->fetchrow_array();
+ return if(!$count);
+
+ my $dna_mappings = $self->dna_mappings();
+
+ my $get_seq_sth = $dbh->prepare
+ ("SELECT substring(sequence,1,?) from $source.dna " .
+ "WHERE dna_id = ?");
+
+ my $store_seq_sth = $dbh->prepare
+ ("INSERT INTO $target.dna (seq_region_id, sequence) " .
+ "VALUES (?,?)");
+
+ my $update_seq_sth = $dbh->prepare
+ ("UPDATE $target.dna " .
+ "SET sequence = concat(sequence, ?) " .
+ "WHERE seq_region_id = ?");
+
+ foreach my $new_id (keys %$dna_mappings) {
+ my $first = 1;
+ foreach my $row (@{$dna_mappings->{$new_id}}) {
+ my ($old_id, $length) = @$row;
+ $get_seq_sth->execute($length, $old_id);
+
+ if(!$get_seq_sth->rows() == 1) {
+ warn("Missing dna sequence for dna id $old_id");
+ next;
+ }
+ my $seq = $get_seq_sth->fetchrow_arrayref->[0];
+
+ if($first) {
+ #$self->debug("storing sequence of length " . length($seq) .
+ # "for seq_region $new_id");
+ $store_seq_sth->execute($new_id, $seq);
+ $first = 0;
+ } else {
+ $update_seq_sth->execute($seq, $new_id);
+ #$self->debug("concatting sequence to length " . length($seq));
+ }
+ }
+ }
+
+ $get_seq_sth->finish();
+ $store_seq_sth->finish();
+ $update_seq_sth->finish();
+ return;
+}
+
+
+#
+# override the transfer features so that features which are on fake contigs
+# that were merged into scaffolds can have their coordinates adjusted
+#
+
+sub transfer_features {
+ my $self = shift;
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $self->SUPER::transfer_features();
+
+ my $contig_mappings = $self->contig_mappings();
+
+ my @tables = qw(simple_feature
+ repeat_feature
+ protein_align_feature
+ dna_align_feature
+ marker_feature
+ prediction_transcript);
+
+ foreach my $table (@tables) {
+
+ $self->debug("DanioRerio Specific: updating $table coordinates");
+
+ my $sth = $dbh->prepare
+ ("UPDATE $target.$table " .
+ "SET seq_region_id = ?, " .
+ " seq_region_start = seq_region_start + ?, " .
+ " seq_region_end = seq_region_end + ? " .
+ "WHERE seq_region_id = ?");
+
+ foreach my $old_id (keys %$contig_mappings) {
+ my ($new_id, $offset) = @{$contig_mappings->{$old_id}};
+ if($offset > 1) {
+ $sth->execute($new_id, $offset, $offset, $old_id)
+ }
+ }
+ }
+}
+
+
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("DanioRerio Specific: no assembly data");
+
+ return;
+}
+
+
+
+#
+# override the contig_to_seqregion method so that contigs are given clone
+# names instead
+#
+sub contig_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= 'contig';
+
+ $self->debug("DanioRerio Specific: Transforming contigs " .
+ "into $target_cs_name seq_regions");
+
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ my $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT ctg.contig_id, CONCAT(cln.embl_acc, '.', cln.embl_version), " .
+ " $cs_id, ctg.length " .
+ "FROM $source.contig ctg, $source.clone cln " .
+ "WHERE ctg.clone_id = cln.clone_id");
+
+ $sth->execute();
+ $sth->finish();
+
+ return;
+}
+
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/DrosophilaMelanogaster.pm b/misc-scripts/surgery/SeqStoreConverter/DrosophilaMelanogaster.pm
new file mode 100644
index 0000000000..837774b64f
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/DrosophilaMelanogaster.pm
@@ -0,0 +1,99 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::DrosophilaMelanogaster;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("DrosophilaMelanogaster Specific: creating " .
+ "chunk and chromosome coord systems");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["chromosome" , $ass_def, "top_level,default_version" ],
+ ["chunk", undef , "default_version,sequence_level"]);
+
+ my @assembly_mappings = ("chromosome:$ass_def|chunk");
+
+ my %cs = (gene => 'chromosome',
+ transcript => 'chromosome',
+ exon => 'chromosome',
+ dna_align_feature => 'chunk',
+ protein_align_feature => 'chunk',
+ marker_feature => 'chunk',
+ simple_feature => 'chunk',
+ repeat_feature => 'chunk',
+ qtl_feature => 'chromosome',
+ misc_feature => 'chromosome',
+ prediction_transcript => 'chunk',
+ karyotype => 'chromosome');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $self->debug("Adding assembly.mapping entries to meta table");
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("DrosophilaMelanogaster Specific: creating chunk and " .
+ "chromosome seq_regions");
+
+ $self->contig_to_seq_region('chunk');
+ $self->chromosome_to_seq_region();
+}
+
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("DrosophilaMelanogaster Specific: loading assembly data");
+
+ $self->assembly_contig_chromosome();
+}
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/FuguRubripes.pm b/misc-scripts/surgery/SeqStoreConverter/FuguRubripes.pm
new file mode 100644
index 0000000000..1f80a0ebb0
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/FuguRubripes.pm
@@ -0,0 +1,167 @@
+
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::FuguRubripes;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("FuguRubripes Specific: creating scaffold coord system");
+
+ #
+ # Load the coord system table.
+ # Fugu has only one coord system : scaffold
+ #
+ my $default_assembly = $self->get_default_assembly();
+ my $target = $self->target();
+
+ my $sth = $self->dbh()->prepare
+ ("INSERT INTO $target.coord_system (name, version, attrib)" .
+ "VALUES (?,?,?)");
+ $sth->execute('scaffold', $default_assembly,
+ 'top_level,default_version,sequence_level');
+
+ my $csid = $sth->{'mysql_insertid'};
+
+ $sth->finish();
+
+ #
+ # Load the meta_coord table. Every feature is in scaffold coordinates.
+ #
+ $sth = $self->dbh()->prepare
+ ("INSERT INTO $target.meta_coord (table_name, coord_system_id) " .
+ "VALUES (?,?)");
+
+ my @tables = qw(gene
+ transcript
+ exon
+ dna_align_feature
+ protein_align_feature
+ marker_feature
+ simple_feature
+ repeat_feature
+ qtl_feature
+ misc_feature
+ prediction_transcript
+ karyotype);
+
+ foreach my $table (@tables) {
+ $sth->execute($table, $csid);
+ }
+ $sth->finish();
+}
+
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("FuguRubripes Specific: creating scaffolds");
+
+ $self->contig_to_seq_region('scaffold');
+}
+
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("FuguRubripes Specific: no assembly data needed");
+ #fugu has no assembly table
+}
+
+
+sub transfer_genes {
+ my $self = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ #
+ # This is simple in fugu since all genes are actually in contig (now
+ # renamed scaffold) coords. We don't need joins to the assembly table
+ # and we don't have to worry about stickies
+ #
+
+ $self->debug("FuguRubripes Specific: Building gene table " .
+ "(no chromosomal conversion)");
+
+ #
+ # Transfer the gene table
+ #
+
+ $dbh->do
+ ("INSERT INTO $target.gene " .
+ "SELECT g.gene_id, g.type, g.analysis_id, e.contig_id, " .
+ " MIN(e.contig_start), MAX(e.contig_end), e.contig_strand, " .
+ " g.display_xref_id " .
+ "FROM $source.transcript t, $source.exon_transcript et, " .
+ " $source.exon e, $source.gene g " .
+ "WHERE t.transcript_id = et.transcript_id " .
+ "AND et.exon_id = e.exon_id " .
+ "AND g.gene_id = t.gene_id " .
+ "GROUP BY g.gene_id");
+
+ $self->debug("FuguRubripes Specific: Building transcript table " .
+ "(no chromosomal conversion)");
+
+ #
+ # Transfer transcript table
+ #
+
+ $dbh->do
+ ("INSERT INTO $target.transcript " .
+ "SELECT t.transcript_id, t.gene_id, e.contig_id, " .
+ " MIN(e.contig_start), MAX(e.contig_end), e.contig_strand, " .
+ " t.display_xref_id " .
+ "FROM $source.transcript t, $source.exon_transcript et, " .
+ " $source.exon e " .
+ "WHERE t.transcript_id = et.transcript_id " .
+ "AND et.exon_id = e.exon_id " .
+ "GROUP BY t.transcript_id");
+
+ $self->debug("FuguRubripes Specific: Building exon table " .
+ "(no chromosomal conversion)");
+
+
+ #
+ # Transfer exon table
+ #
+
+ $self->debug("FuguRubripes Specific: Building transcript table " .
+ "(no chromosomal conversion)");
+
+ $dbh->do
+ ("INSERT INTO $target.exon " .
+ "SELECT e.exon_id, e.contig_id, " .
+ " e.contig_start, e.contig_end, e.contig_strand, " .
+ " e.phase, e.end_phase " .
+ "FROM $source.exon e");
+
+
+ #
+ # Transfer translation table
+ #
+
+ $self->debug("Building translation table");
+
+ $dbh->do
+ ("INSERT INTO $target.translation " .
+ "SELECT tl.translation_id, ts.transcript_id, tl.seq_start, " .
+ " tl.start_exon_id, tl.seq_end, tl.end_exon_id " .
+ "FROM $source.transcript ts, $source.translation tl " .
+ "WHERE ts.translation_id = tl.translation_id");
+
+ return;
+}
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/HomoSapiens.pm b/misc-scripts/surgery/SeqStoreConverter/HomoSapiens.pm
new file mode 100644
index 0000000000..664adb9559
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/HomoSapiens.pm
@@ -0,0 +1,47 @@
+
+use strict;
+use warnings;
+
+package SeqStoreConverter::HomoSapiens;
+
+use SeqStoreConverter::BasicConverter;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_attribs {
+ my $self = shift;
+
+ #
+ # Human clones need their htg phase information copied
+ #
+
+ my $source = $self->source();
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ $self->SUPER::create_attribs();
+
+ $self->debug("HomoSapiens specific: Creating HTG Phase seq_region attribs");
+
+ $dbh->do
+ ("INSERT INTO $target.attrib_type( code, name, description ) " .
+ "VALUES ('htg_phase', 'HTG Phase', 'High Throughput Genome Phase')");
+
+
+ $dbh->do
+ ("INSERT INTO $target.seq_region_attrib( seq_region_id, attrib_type_id, " .
+ "value) " .
+ "SELECT tmp_cln.new_id, attrib_type.attrib_type_id, cln.htg_phase " .
+ "FROM $target.tmp_cln_map tmp_cln, $target.attrib_type attrib_type, " .
+ " $source.clone cln " .
+ "WHERE cln.clone_id = tmp_cln.old_id " .
+ "AND attrib_type.code = 'htg_phase'");
+
+ return;
+}
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/MusMusculus.pm b/misc-scripts/surgery/SeqStoreConverter/MusMusculus.pm
new file mode 100644
index 0000000000..e21530dd4b
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/MusMusculus.pm
@@ -0,0 +1,211 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::MusMusculus;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("MusMusculus Specific: loading assembly data");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["chromosome" , $ass_def, "top_level,default_version" ],
+ ["supercontig", undef , "default_version" ],
+ ['clone' , undef , 'default_version' ],
+ ["contig" , undef , "default_version,sequence_level"]);
+
+ my @assembly_mappings = ("chromosome:$ass_def|contig",
+ "supercontig|contig",
+ "clone|contig");
+
+ my %cs = (gene => 'chromosome',
+ transcript => 'chromosome',
+ exon => 'chromosome',
+ dna_align_feature => 'contig',
+ protein_align_feature => 'contig',
+ marker_feature => 'contig',
+ simple_feature => 'contig',
+ repeat_feature => 'contig',
+ qtl_feature => 'chromosome',
+ misc_feature => 'chromosome',
+ prediction_transcript => 'contig',
+ karyotype => 'chromosome');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $self->debug("Adding assembly.mapping entries to meta table");
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("MusMusculus Specific: creating contig, " .
+ "clone, chromosome and supercontig seq_regions");
+
+ $self->contig_to_seq_region();
+ $self->clone_to_seq_region();
+ $self->chromosome_to_seq_region();
+ $self->supercontig_to_seq_region();
+}
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("MusMusculus Specific: loading assembly data");
+
+ $self->assembly_contig_chromosome();
+ $self->assembly_contig_clone();
+ $self->assembly_contig_supercontig();
+}
+
+#
+# Override contig_to_seq_region and clone_to_seq_region to provide
+# mouse specific behaviour
+#
+
+sub contig_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= 'contig';
+
+ $self->debug("MusMusculus Specific: Transforming contigs into " .
+ "$target_cs_name seq_regions");
+
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ #There are two types of contigs in mouse:
+
+ #
+ # Contigs which form BAC clones
+ #
+ my $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT contig_id, name, $cs_id, length " .
+ "FROM $source.contig " .
+ "WHERE name not like 'C%'");
+
+ $sth->execute();
+ $sth->finish();
+
+ #
+ # Contigs which were created from whole genome shotgun
+ #
+ $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT ctg.contig_id, cln.name, $cs_id, length " .
+ "FROM $source.contig ctg, $source.clone cln " .
+ "WHERE ctg.clone_id = cln.clone_id " .
+ "AND ctg.name like 'C%'");
+
+ $sth->execute();
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub clone_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ # target coord_system will have a different ID
+ $target_cs_name ||= "clone";
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ $self->debug("MusMusculus Specific:Transforming clones " .
+ "into $target_cs_name seq_regions");
+
+ #
+ # We don't want to make clones out of the WGS contigs, only out of
+ # the actual BACs with proper embl accessions
+ #
+ my $select_sth = $dbh->prepare
+ ("SELECT cl.clone_id,
+ CONCAT(cl.embl_acc, '.', cl.embl_version),
+ MAX(ctg.embl_offset)+ctg.length-1
+ FROM $source.clone cl, $source.contig ctg
+ WHERE cl.clone_id = ctg.clone_id
+ AND cl.embl_acc not like 'C%'
+ GROUP BY ctg.clone_id");
+
+ $select_sth->execute();
+
+ my ($clone_id, $embl_acc, $length);
+ $select_sth->bind_columns(\$clone_id, \$embl_acc, \$length);
+
+ my $insert_sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region (name, coord_system_id, length) " .
+ "VALUES(?,?,?)");
+
+ my $tmp_insert_sth = $dbh->prepare
+ ("INSERT INTO $target.tmp_cln_map (old_id, new_id) VALUES (?, ?)");
+
+ while ($select_sth->fetch()) {
+ $insert_sth->execute("$embl_acc", $cs_id, $length);
+
+ #store mapping of old -> new ids in temp table
+ $tmp_insert_sth->execute($clone_id, $insert_sth->{'mysql_insertid'});
+ }
+
+ $select_sth->finish();
+ $insert_sth->finish();
+ $tmp_insert_sth->finish();
+
+ return;
+}
+
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/PanTroglodytes.pm b/misc-scripts/surgery/SeqStoreConverter/PanTroglodytes.pm
new file mode 100644
index 0000000000..8ead95b2bf
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/PanTroglodytes.pm
@@ -0,0 +1,96 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::PanTroglodytes;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("PanTroglodytes Specific: loading assembly data");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["scaffold" , $ass_def, "top_level,default_version" ],
+ ["contig" , undef , "default_version,sequence_level"]);
+
+ my @assembly_mappings = ("scaffold:$ass_def|contig");
+
+ my %cs = (gene => 'scaffold',
+ transcript => 'scaffold',
+ exon => 'scaffold',
+ dna_align_feature => 'contig',
+ protein_align_feature => 'contig',
+ marker_feature => 'contig',
+ simple_feature => 'contig',
+ repeat_feature => 'contig',
+ qtl_feature => 'scaffold',
+ misc_feature => 'scaffold',
+ prediction_transcript => 'contig',
+ karyotype => 'chromosome');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $self->debug("Adding assembly.mapping entries to meta table");
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("PanTroglodytes Specific: creating contig, " .
+ "clone, chromosome and supercontig seq_regions");
+
+ $self->contig_to_seq_region();
+ $self->chromosome_to_seq_region('scaffold');
+}
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("PanTroglodytes Specific: loading assembly data");
+
+ $self->assembly_contig_chromosome();
+}
+
+1;
diff --git a/misc-scripts/surgery/SeqStoreConverter/RattusNorvegicus.pm b/misc-scripts/surgery/SeqStoreConverter/RattusNorvegicus.pm
new file mode 100644
index 0000000000..d445b674f7
--- /dev/null
+++ b/misc-scripts/surgery/SeqStoreConverter/RattusNorvegicus.pm
@@ -0,0 +1,135 @@
+use strict;
+use warnings;
+
+use SeqStoreConverter::BasicConverter;
+
+package SeqStoreConverter::RattusNorvegicus;
+
+use vars qw(@ISA);
+
+@ISA = qw(SeqStoreConverter::BasicConverter);
+
+
+sub create_coord_systems {
+ my $self = shift;
+
+ $self->debug("RattusNorvegicus Specific: loading assembly data");
+
+ my $target = $self->target();
+ my $dbh = $self->dbh();
+
+ my $ass_def = $self->get_default_assembly();
+
+ my @coords =
+ (["chromosome" , $ass_def, "top_level,default_version" ],
+ ["supercontig", undef , "default_version" ],
+ ["contig" , undef , "default_version,sequence_level"]);
+
+ my @assembly_mappings = ("chromosome:$ass_def|contig",
+ "chromosome:$ass_def|supercontig");
+
+ my %cs = (gene => 'chromosome',
+ transcript => 'chromosome',
+ exon => 'chromosome',
+ dna_align_feature => 'contig',
+ protein_align_feature => 'contig',
+ marker_feature => 'contig',
+ simple_feature => 'contig',
+ repeat_feature => 'contig',
+ qtl_feature => 'chromosome',
+ misc_feature => 'chromosome',
+ prediction_transcript => 'contig',
+ karyotype => 'chromosome');
+
+ $self->debug("Building coord_system table");
+
+ my $sth = $dbh->prepare("INSERT INTO $target.coord_system " .
+ "(name, version, attrib) VALUES (?,?,?)");
+
+ my %coord_system_ids;
+
+ foreach my $cs (@coords) {
+ $sth->execute(@$cs);
+ $coord_system_ids{$cs->[0]} = $sth->{'mysql_insertid'};
+ }
+ $sth->finish();
+
+ $self->debug("Building meta_coord table");
+ $sth = $dbh->prepare("INSERT INTO $target.meta_coord VALUES (?, ?)");
+ foreach my $val (keys %cs) {
+ $sth->execute($val, $coord_system_ids{$cs{$val}});
+ }
+ $sth->finish();
+
+ $self->debug("Adding assembly.mapping entries to meta table");
+
+ $sth = $dbh->prepare("INSERT INTO $target.meta(meta_key, meta_value) " .
+ "VALUES ('assembly.mapping', ?)");
+
+ foreach my $mapping (@assembly_mappings) {
+ $sth->execute($mapping);
+ }
+
+ $sth->finish();
+
+ return;
+}
+
+
+
+sub create_seq_regions {
+ my $self = shift;
+
+ $self->debug("RattusNorvegicus Specific: creating contig, " .
+ "chromosome and supercontig seq_regions");
+
+ $self->contig_to_seq_region();
+ $self->chromosome_to_seq_region();
+ $self->supercontig_to_seq_region();
+}
+
+sub create_assembly {
+ my $self = shift;
+
+ $self->debug("RattusNorvegicus Specific: loading assembly data");
+
+ $self->assembly_contig_chromosome();
+ $self->assembly_supercontig_chromosome();
+}
+
+
+#
+# override the contig_to_seqregion method so that contigs are given clone
+# names instead
+#
+sub contig_to_seq_region {
+ my $self = shift;
+ my $target_cs_name = shift;
+
+ my $target = $self->target();
+ my $source = $self->source();
+ my $dbh = $self->dbh();
+
+ $target_cs_name ||= 'contig';
+
+ $self->debug("RattusNorvegicus Specific: Transforming contigs " .
+ "into $target_cs_name seq_regions");
+
+ my $cs_id = $self->get_coord_system_id($target_cs_name);
+
+ my $sth = $dbh->prepare
+ ("INSERT INTO $target.seq_region " .
+ "SELECT ctg.contig_id, cln.embl_acc, " .
+ " $cs_id, ctg.length " .
+ "FROM $source.contig ctg, $source.clone cln " .
+ "WHERE ctg.clone_id = cln.clone_id");
+
+ $sth->execute();
+ $sth->finish();
+
+ return;
+}
+
+
+
+1;
diff --git a/misc-scripts/surgery/convert_seqstore.pl b/misc-scripts/surgery/convert_seqstore.pl
new file mode 100644
index 0000000000..359528192f
--- /dev/null
+++ b/misc-scripts/surgery/convert_seqstore.pl
@@ -0,0 +1,124 @@
+use strict;
+use warnings;
+
+use Getopt::Long;
+use Cwd;
+
+use vars qw(@INC);
+
+#effectively add this directory to the PERL5LIB automatically
+my $dir = cwd() . '/' . __FILE__;
+my @d = split(/\//, $dir);
+pop(@d);
+$dir = join('/', @d);
+unshift @INC, $dir;
+
+
+my ($file, $user, $password, $verbose, $force, $help, $schema, $limit);
+
+GetOptions ('file=s' => \$file,
+ 'schema=s' => \$schema,
+ 'user=s' => \$user,
+ 'password=s' => \$password,
+ 'verbose' => \$verbose,
+ 'force' => \$force,
+ 'limit=s' => \$limit,
+ 'help' => sub { &show_help(); exit 1;} );
+
+usage("-file option is required") if(!$file);
+usage("-schema option is required") if(!$schema);
+usage() if($help);
+
+open(FILE, $file) or die("Could not open input file '$file'");
+
+
+
+my @all_species_converters;
+
+while( my $line = <FILE> ) {
+ chomp($line);
+ next if $line =~ /^#/;
+ next if !$line;
+
+ my ( $species, $host, $source_db_name, $target_db_name ) =
+ split( "\t", $line );
+
+ my $converter;
+
+ eval "require SeqStoreConverter::$species";
+
+ if($@) {
+ warn("Could not require conversion module SeqStoreConverter::$species\n" .
+ "Using SeqStoreConverter::BasicConverter instead:\n$@");
+
+ require SeqStoreConverter::BasicConverter;
+ $species = "BasicConverter";
+ }
+
+ {
+ no strict 'refs';
+
+ $converter = "SeqStoreConverter::$species"->new
+ ( $user, $password, $host, $source_db_name, $target_db_name,
+ $schema, $force, $verbose, $limit );
+ }
+
+ push @all_species_converters, $converter;
+}
+
+for my $converter ( @all_species_converters ) {
+ $converter->debug( "\n\n*** converting " . $converter->source . " to " .
+ $converter->target() . " ***");
+ $converter->transfer_meta();
+ $converter->create_coord_systems();
+ $converter->create_seq_regions();
+ $converter->create_assembly();
+ $converter->create_attribs();
+
+ $converter->transfer_dna();
+ $converter->transfer_genes();
+ $converter->transfer_prediction_transcripts();
+ $converter->transfer_features();
+ $converter->transfer_stable_ids();
+ $converter->copy_other_tables();
+}
+
+
+print STDERR "*** All finished ***\n";
+
+sub usage {
+ my $msg = shift;
+
+ print STDERR "$msg\n\n" if($msg);
+
+ print STDERR <<EOF;
+usage: perl convert_seqstore <options>
+
+options: -file <input_file> input file with tab delimited 'species',
+ 'host', 'source_db', 'target_db' values
+ on each line
+
+ -schema <table_file> file containing SQL schema definition
+
+ -user <user> a mysql db user with read/write priveleges
+
+ -password <password> the mysql user's password
+
+ -verbose print out debug statements
+
+ -force replace any target dbs that already exists
+
+ -limit <num_rows> limit the number of features transfered to
+ speed up testing
+
+ -help display this message
+
+example: perl convert_seqstore.pl -file converter.input \\
+ -schema ../../sql/table.sql -user ensadmin -password secret \\
+ -force -verbose
+
+EOF
+#'
+
+ exit;
+}
diff --git a/misc-scripts/surgery/converter.input b/misc-scripts/surgery/converter.input
new file mode 100644
index 0000000000..6be8023588
--- /dev/null
+++ b/misc-scripts/surgery/converter.input
@@ -0,0 +1,9 @@
+#HomoSapiens ecs1d mcvicker_old_schema mcvicker_new_schema
+#FuguRubripes ecs2f fugu_rubripes_core_18_2 mcvicker_new_fugu
+CaenorhabditisElegans ecs2f caenorhabditis_elegans_core_18_102 mcvicker_new_elegans
+#CaenorhabditisBriggsae ecs2f caenorhabditis_briggsae_core_18_25 mcvicker_new_briggsae
+#RattusNorvegicus ecs2f rattus_norvegicus_core_18_3 mcvicker_new_rat
+#DrosophilaMelanogaster ecs2f drosophila_melanogaster_core_18_3a mcvicker_new_fly
+#MusMusculus ecs2f mus_musculus_core_18_30 mcvicker_new_mouse
+#DanioRerio ecs2f danio_rerio_core_18_2 mcvicker_new_zebrafish
+#AnophelesGambiae ecs2f anopheles_gambiae_core_18_2a mcvicker_new_mosquito
diff --git a/misc-scripts/surgery/set_nonredundant_attribs.pl b/misc-scripts/surgery/set_nonredundant_attribs.pl
new file mode 100644
index 0000000000..7ee3696f0c
--- /dev/null
+++ b/misc-scripts/surgery/set_nonredundant_attribs.pl
@@ -0,0 +1,235 @@
+# Figure out which seq_regions are non-redundant and set the appropriate
+# attribute in seq_region_attrib
+
+use strict;
+use warnings;
+
+use DBI;
+use Getopt::Long;
+
+use Bio::EnsEMBL::DBSQL::DBAdaptor;
+use Bio::EnsEMBL::DBSQL::SliceAdaptor;
+
+my ($host, $port, $user, $password, $db, $verbose, $check);
+$host = "127.0.0.1";
+$port = 3306;
+$password = "";
+$user = "ensro";
+
+GetOptions ('host=s' => \$host,
+ 'user=s' => \$user,
+ 'password=s' => \$password,
+ 'port=s' => \$port,
+ 'db=s' => \$db,
+ 'verbose' => \$verbose,
+ 'check' => \$check,
+ 'help' => sub { &show_help(); exit 1;} );
+
+die "Host must be specified" unless $host;
+die "Database must be specified" unless $db;
+
+my $dbi = DBI->connect("dbi:mysql:host=$host;port=$port;database=$db", "$user", "$password", {'RaiseError' => 1}) || die "Can't connect to target DB";
+
+# ----------------------------------------
+
+# check that there is an entry in the attrib_type table for nonredundant
+# if there is, cache the ID for later; if not, make one
+my $nr_attrib_type_id;
+my $sth = $dbi->prepare("SELECT attrib_type_id FROM attrib_type WHERE code='nonredundant'");
+$sth->execute();
+while ( my @row= $sth->fetchrow_array()) {
+ $nr_attrib_type_id = $row[0];
+}
+$sth->finish();
+
+if ($nr_attrib_type_id) {
+
+ debug("Attribute with name nonredundant already set in attrib_type with attrib_type_id " . $nr_attrib_type_id);
+
+} else {
+
+ debug("Attribute with name nonredundant not set in attrib_type; adding ...");
+
+ $sth = $dbi->prepare("INSERT INTO attrib_type (code, name) VALUES ('nonredundant', 'Non-redundant sequence region')");
+ $sth->execute();
+
+ $nr_attrib_type_id = $sth->{mysql_insertid};
+
+ debug("Added nonredundant attribute with ID " . $nr_attrib_type_id);
+
+}
+
+# ----------------------------------------
+
+my $db_adaptor = new Bio::EnsEMBL::DBSQL::DBAdaptor(-user => $user,
+ -dbname => $db,
+ -host => $host,
+ -port => $port,
+ -pass => $password,
+ -driver => 'mysql' );
+
+my $slice_adaptor = $db_adaptor->get_SliceAdaptor();
+
+# Assume all entries in the top-level co-ordinate system are non-redundant
+my @toplevel = @{$slice_adaptor->fetch_all('toplevel')};
+debug("Got " . @toplevel . " sequence regions in the top-level co-ordinate system");
+set_nr_attribute($slice_adaptor, $nr_attrib_type_id, $dbi, @toplevel);
+
+# Rest of the co-ordinate systems, in "ascending" order
+my @coord_systems = get_coord_systems_in_order($db_adaptor, $slice_adaptor);
+
+debug("Starting pair-wise co-ordinate system comparison");
+
+my @nr_slices; # will store non-redundant ones for later
+
+for (my $lower_cs_idx = 0; $lower_cs_idx < @coord_systems; $lower_cs_idx++) {
+ for (my $higher_cs_idx = $lower_cs_idx+1; $higher_cs_idx < @coord_systems; $higher_cs_idx++) {
+
+ my $higher_cs = $coord_systems[$higher_cs_idx];
+ my $lower_cs = $coord_systems[$lower_cs_idx];
+
+ debug("$lower_cs:$higher_cs");
+
+ # we are interested in the slices that do *not* project onto the "higher" coordinate system
+ my @slices = @{$slice_adaptor->fetch_all($lower_cs)};
+
+ my $projected_hit = 0;
+ my $projected_miss = 0;
+
+ foreach my $slice (@slices) {
+ my @projected = @{$slice->project($higher_cs)};
+ if (@projected > 0) {
+ $projected_hit++;
+ } else {
+ $projected_miss++;
+ push @nr_slices, $slice;
+ }
+ undef @projected;
+ }
+ debug ("Projecting " . $lower_cs . " onto " . $higher_cs . ": " .
+ $projected_hit . " hit, " . $projected_miss . " miss out of " . @slices . " total");
+
+ undef @slices;
+
+ }
+}
+
+set_nr_attribute($slice_adaptor, $nr_attrib_type_id, $dbi, @nr_slices);
+
+#----------------------------------------
+
+check_non_redundant($slice_adaptor) if $check;
+
+$sth->finish();
+$dbi->disconnect();
+
+# ----------------------------------------
+# Get all the co-ordinate systems in order
+# Order is descending average length
+# Return an array of co-ordinate system names
+
+sub get_coord_systems_in_order() {
+
+ my $db_adaptor = shift;
+ my $slice_adaptor = shift;
+
+ debug("Ordering co-ordinate systems by average length");
+
+ my $cs_adaptor = $db_adaptor->get_CoordSystemAdaptor();
+ my @coord_system_objs = @{$cs_adaptor->fetch_all()};
+
+ # Calculate average lengths
+ my %lengths;
+ foreach my $cs (@coord_system_objs) {
+ my @slices = @{$slice_adaptor->fetch_all($cs->name())};
+ my $total_len = 0;
+ foreach my $slice (@slices) {
+ $total_len += $slice->length();
+ }
+ if ($total_len > 0) {
+ $lengths{$cs->name()} = $total_len / scalar(@slices);
+ } else {
+ $lengths{$cs->name()} = 0;
+ print "Warning - total length for " . $cs->name() . " is zero!\n";
+ }
+ }
+
+ my @coord_systems = sort { $lengths{$a} <=> $lengths{$b} } keys %lengths;
+
+ foreach my $cs_name (@coord_systems) {
+ debug("Co-ord system: " . $cs_name . " Average length: " . int $lengths{$cs_name});
+ }
+
+ debug("Got co-ordinate systems in order: " . join(', ', @coord_systems));
+
+ return @coord_systems;
+
+}
+
+# ----------------------------------------------------------------------
+# Misc / utility functions
+
+sub show_help {
+
+ print "Usage: perl set_non_redundant_attribs.pl {options}\n";
+ print "Where options are:\n";
+ print " --host {hostname} The database host.\n";
+ print " --user {username} The database user. Must have write permissions\n";
+ print " --password {pass} The password for user, if required.\n";
+ print " --port {folder} The database port to use.\n";
+ print " --db {schema} The name of the database\n";
+ print " --check Read back non-redundant slices from SliceAdaptor\n";
+ print " --verbose Print extra output information\n";
+
+}
+
+# ----------------------------------------------------------------------
+
+sub debug {
+
+ my $str = shift;
+
+ print $str . "\n" if $verbose;
+
+}
+
+# ----------------------------------------------------------------------
+
+# Set the "nonredundant" attribute on a Slice or group of Slices
+# arg 1: SliceAdaptor
+# arg 2: internal ID of 'nonredundant' attrib_type
+# arg 3: DB connection
+# arg 3..n: Slices
+
+sub set_nr_attribute {
+
+ my ($slice_adaptor, $nr_attrib_type_id, $dbi, @targets) = @_;
+
+ debug("Setting nonredundant attribute on " . @targets . " sequence regions");
+
+ my $sth = $dbi->prepare("INSERT INTO seq_region_attrib (seq_region_id, attrib_type_id) VALUES (?,?)");
+
+ foreach my $slice (@targets) {
+
+ my $seq_region_id = $slice_adaptor->get_seq_region_id($slice);
+
+ $sth->execute($seq_region_id, $nr_attrib_type_id);
+
+ }
+
+ $sth->finish();
+
+}
+
+# ----------------------------------------------------------------------
+
+sub check_non_redundant {
+
+ my $slice_adaptor = shift;
+
+ my @all = @{$slice_adaptor->fetch_all_non_redundant()};
+
+ print "Got " . @all . " non-redundant seq_regions from SliceAdaptor\n";
+
+}
+
diff --git a/modules/Bio/EnsEMBL/Analysis.pm b/modules/Bio/EnsEMBL/Analysis.pm
index cdccbcbc52..512741365a 100755
--- a/modules/Bio/EnsEMBL/Analysis.pm
+++ b/modules/Bio/EnsEMBL/Analysis.pm
@@ -49,16 +49,17 @@ Internal methods are usually preceded with a _
=cut
-# Let the code begin...
-
package Bio::EnsEMBL::Analysis;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::Root;
-# Inherits from the base bioperl object
-@ISA = qw(Bio::EnsEMBL::Root);
+use Bio::EnsEMBL::Storable;
+
+use Bio::EnsEMBL::Utils::Exception qw(throw);
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+@ISA = qw(Bio::EnsEMBL::Storable);
=head2 new
@@ -95,7 +96,7 @@ sub new {
$program_file, $gff_source, $gff_feature, $module, $module_version,
$parameters, $created, $logic_name ) =
- $self->_rearrange([qw(ID
+ rearrange([qw(ID
ADAPTOR
DB
DB_VERSION
@@ -132,50 +133,6 @@ sub new {
}
-=head2 adaptor
-
- Arg [1] : Bio::EnsEMBL::DBSQL::AnalysisAdaptor $analysis_adaptor
- Example : none
- Description: get/set for thus objects Adaptor
- Returntype : Bio::EnsEMBL::DBSQL::AnalysisAdaptor
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub adaptor {
- my ($self,$arg) = @_;
-
- $self->{_adaptor} ||= undef;
-
- if (defined($arg)) {
- $self->{_adaptor} = $arg;
- }
- return $self->{_adaptor};
-}
-
-
-=head2 dbID
-
- Arg [1] : int $dbID
- Example : none
- Description: get/set for the database internal id
- Returntype : int
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub dbID {
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
- $self->{_dbid} = $arg;
- }
- return $self->{_dbid};
-}
-
-
=head2 db
Arg [1] : string $db
@@ -500,7 +457,7 @@ sub has_database{
sub compare {
my ($self, $ana ) = @_;
- $self->throw("Object is not a Bio::EnsEMBL::Analysis")
+ throw("Object is not a Bio::EnsEMBL::Analysis")
unless $ana->isa("Bio::EnsEMBL::Analysis");
my $detail = 0;
@@ -515,7 +472,7 @@ sub compare {
$detail = 1;
}
# if given anal is different from this, defined or not, then its different
- if( defined $ana->$methodName() && defined $self->$methodName &&
+ if( defined $ana->$methodName() &&
( $self->$methodName() ne $ana->$methodName() )) {
return -1;
}
diff --git a/modules/Bio/EnsEMBL/AssemblyExceptionFeature.pm b/modules/Bio/EnsEMBL/AssemblyExceptionFeature.pm
new file mode 100644
index 0000000000..f8cc0125d1
--- /dev/null
+++ b/modules/Bio/EnsEMBL/AssemblyExceptionFeature.pm
@@ -0,0 +1,168 @@
+#
+# Ensembl module for Bio::EnsEMBL::AssemblyExceptionFeature
+#
+# Cared for by Ewan Birney <birney@ebi.ac.uk>
+#
+# Copyright Ewan Birney
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::AssemblyExceptionFeature - A feature that represents an assembly exception
+
+=head1 SYNOPSIS
+
+use Bio::EnsEMBL::AssemblyExceptionFeature;
+
+$feature = Bio::EnsEMBL::AssemblyExceptionFeature->new(-start => 100,
+ -end => 220,
+ -type => 'HAP',
+ -slice => $slice,
+ -adaptor => $adaptor);
+
+=head1 DESCRIPTION
+
+Certain features, e.g. Haplotypes and PARs, are represented as "exceptions" to the
+normal assembly. This class represents such features.
+
+=head1 AUTHOR - Glenn Proctor
+
+This modules is part of the Ensembl project http://www.ensembl.org
+
+Email birney@ebi.ac.uk
+
+Describe contact details here
+
+=head1 APPENDIX
+
+The rest of the documentation details each of the object methods. Internal
+methods are usually preceded with a _
+
+=cut
+
+use strict;
+
+package Bio::EnsEMBL::AssemblyExceptionFeature;
+
+use vars qw(@ISA);
+
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+@ISA = qw(Bio::EnsEMBL::Feature);
+
+
+=head2 new
+
+ Arg [TYPE] : The type (e.g. HAP for haplotype, PAR for PAR)
+ Arg [...] : Named arguments passed to superclass
+ Example : $feature = Bio::EnsEMBL::AssemblyExceptionFeature->new
+ (-start => 1,
+ -end => 100,
+ -slice => $slice,
+ -alternate_slice => $alt_slice,
+ -adaptor => $adaptor,
+ -type => 'HAP')
+ Description: Constructs a new Bio::EnsEMBL::Feature. Generally subclasses
+ of this method are instantiated, rather than this class itself.
+ Returntype : Bio::EnsEMBL::Feature
+ Exceptions : Thrown on invalid -SLICE arguments
+ Caller : general, subclass constructors
+
+=cut
+
+sub new {
+
+ my $caller = shift;
+
+ # allow this to be called as class or object method
+ my $class = ref($caller) || $caller;
+ my $self = $class->SUPER::new(@_);
+
+ my ($type, $alternate_slice) = rearrange(['TYPE', 'ALTERNATE_SLICE'],@_);
+ $self->{'type'} = $type;
+ $self->{'alternate_slice'} = $alternate_slice;
+
+ return $self;
+}
+
+
+sub new_fast {
+ my $class = shift;
+ my $hashref = shift;
+ return bless $hashref, $class;
+}
+
+
+=head2 type
+
+ Arg [1] : (optional) string $value
+ Example : $type = $assembly_exception_feature->type();
+ Description: Getter/Setter for the type associated with this
+ feature.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub type {
+
+ my $self = shift;
+
+ $self->{'type'} = shift if(@_);
+
+ return $self->{'type'};
+}
+
+
+=head2 alternate_slice
+
+ Arg [1] : (optional) string $value
+ Example : $alt_slice = $assembly_exception_feature->alternate_slice();
+ Description: Getter/Setter for the alternate slice associated with this feature.
+ The alternate slice represents the "other side" of the AssemblyExceptionFeature.
+ Returntype : Bio::EnsEMBL::Slice
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub alternate_slice {
+
+ my $self = shift;
+
+ $self->{'alternate_slice'} = shift if(@_);
+
+ return $self->{'alternate_slice'};
+}
+
+
+
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $aef->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For assembly exception features
+ this is the name of the alternate seqregion or '' if the
+ alternate slice is not defined.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
+
+=cut
+
+sub display_id {
+ my $self = shift;
+ my $slice = $self->{'alternate_slice'};
+ return '' if(!$slice);
+ return $slice->seq_region_name();
+}
+
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/AssemblyMapper.pm b/modules/Bio/EnsEMBL/AssemblyMapper.pm
index c89cb7c3a7..683dcd3a31 100644
--- a/modules/Bio/EnsEMBL/AssemblyMapper.pm
+++ b/modules/Bio/EnsEMBL/AssemblyMapper.pm
@@ -13,31 +13,37 @@
=head1 NAME
Bio::EnsEMBL::AssemblyMapper -
-Handles mapping from raw contigs to assembly coordinates
+Handles mapping between two coordinate systems using the information stored in
+the assembly table
=head1 SYNOPSIS
+ $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+ $asma = $db->get_AssemblyMapperAdaptor();
+ $csa = $db->get_CoordSystemAdaptor();
- $map_adaptor = $dbadaptor->get_AssemblyMapperAdaptor();
- $mapper = $map_adaptor->fetch_by_type('UCSC');
+ my $chr_cs = $cs_adaptor->fetch_by_name('chromosome', 'NCBI33');
+ my $ctg_cs = $cs_adaptor->fetch_by_name('contig');
- $mapper->register_region('chr1', 1, 100000);
+ $asm_mapper = $map_adaptor->fetch_by_CoordSystems($cs1, $cs2);
- $mapper->register_region_around_contig(30001, 1000, 1000);
+ #map to contig coordinate system from chromosomal
+ @ctg_coords = $asm_mapper->map('X', 1_000_000, 2_000_000, 1, $chr_cs);
- my @chr_coordlist = $mapper->map_coordinates_to_assembly(627012, 2, 5, -1);
+ #map to chromosome coordinate system from contig
+ @chr_coords = $asm_mapper->map('AL30421.1.200.92341',100,10000,-1,$ctg_cs);
- my @raw_coordlist = $mapper->map_coordinates_to_rawcontig("1",10002,10020);
+ #list contig names for a region of chromsome
+ @ctg_ids = $asm_mapper->list_ids('13', 1_000_000, 1, $chr_cs);
- my @cid_list = $mapper->list_contig_ids("1", 10002, 10020);
+ #list chromosome names for a contig region
+ @chr_ids = $asm_mapper->list_ids('AL30421.1.200.92341',1,1000,-1,$ctg_cs);
=head1 DESCRIPTION
-The AssemblyMapper is a database aware mapper which handles the raw
-contig to assembly mapping. It allows mappings both from raw contigs
-to assembly coordinates and from assembly coordinates back to raw
-contigs.
-
-It extracts the mapping dynamically from the database
+The AssemblyMapper is a database aware mapper which faciliates conversion
+of coordinates between any two coordinate systems with an relationship
+explicitly defined in the assembly table. In the future it may be possible to
+perform multiple step (implicit) mapping between coordinate systems.
It is implemented using the Bio::EnsEMBL::Mapper object, which is a generic
mapper object between disjoint coordinate systems.
@@ -46,361 +52,474 @@ mapper object between disjoint coordinate systems.
Post general queries to B<ensembl-dev@ebi.ac.uk>
-=head1 APPENDIX
-
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-
package Bio::EnsEMBL::AssemblyMapper;
-use vars qw(@ISA);
-use strict;
-# Object preamble - inheriets from Bio::EnsEMBL::Root
+use strict;
+use warnings;
-use Bio::EnsEMBL::Root;
use Bio::EnsEMBL::Mapper;
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate);
-@ISA = qw(Bio::EnsEMBL::Root);
-
+my $ASSEMBLED = 'assembled';
+my $COMPONENT = 'component';
=head2 new
Arg [1] : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
- Arg [2] : string $type
- The type of assembly mapper
- Example : $mapper = new Bio::EnsEMBL::AssemblyMapper($adaptor,'assembly');
- Description: Creates a new AssemblyMapper object.
- Returntype : Bio::EnsEMBL::AssemblyMapper;
- Exceptions : thrown if arg count is wrong
- Caller : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Arg [2] : Bio::EnsEMBL::CoordSystem $asm_cs
+ Arg [3] : Bio::EnsEMBL::CoordSystem $cmp_cs
+ Example : Should use AssemblyMapperAdaptor->fetch_by_CoordSystems
+ Description: Creates a new AssemblyMapper
+ Returntype : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Exceptions : thrown if multiple coord_systems are provided
+ Caller : AssemblyMapperAdaptor
=cut
sub new {
- my($class,$adaptor,$type) = @_;
+ my ($caller,$adaptor,@coord_systems) = @_;
+
+ my $class = ref($caller) || $caller;
my $self = {};
- bless $self,$class;
+ bless $self, $class;
- if( !defined $type ) {
- $self->throw("Must have adaptor, type for the assembly mapper");
- }
- $self->{'_contig_register'} = {};
$self->adaptor($adaptor);
- $self->_type($type);
- my $mapper = Bio::EnsEMBL::Mapper->new('rawcontig','assembly');
- $self->_mapper($mapper);
- return $self;
-}
+ if(@coord_systems != 2) {
+ throw('Can only map between 2 coordinate systems. ' .
+ scalar(@coord_systems) . ' were provided');
+ }
+ # Set the component and assembled coordinate systems
+ $self->{'asm_cs'} = $coord_systems[0];
+ $self->{'cmp_cs'} = $coord_systems[1];
+ #we load the mapper calling the 'ASSEMBLED' the 'from' coord system
+ #and the 'COMPONENT' the 'to' coord system
+ $self->{'mapper'} = Bio::EnsEMBL::Mapper->new($ASSEMBLED, $COMPONENT);
-=head2 map_coordinates_to_assembly
+ return $self;
+}
- Arg 1 : int $contig_id
- raw contig internal ID
- Arg 2 : int $start
- start position on raw contig
- Arg 3 : int $end
- end position on raw contig
- Arg 4 : int $strand
- raw contig orientation (+/- 1)
- Example : none
- Description: takes RawContig coordinates and remaps it
- to Assembly coordinates. Areas that dont map produce
- Gap objects.
- Returntype : list of Bio::EnsEMBL::Mapper::Coordinate,
- Bio::EnsEMBL::Mapper::Gap
- Exceptions : throws if args are not numeric
+
+=head2 map
+
+ Arg [1] : string $frm_seq_region
+ The name of the sequence region to transform FROM
+ Arg [2] : int $frm_start
+ The start of the region to transform FROM
+ Arg [3] : int $frm_end
+ The end of the region to transform FROM
+ Arg [4] : int $strand
+ The strand of the region to transform FROM
+ Arg [5] : Bio::EnsEMBL::CoordSystem
+ The coordinate system to transform FROM
+ Example : @coords = $asm_mapper->map('X', 1_000_000, 2_000_000,
+ 1, $chr_cs);
+ Description: Transforms coordinates from one coordinate system
+ to another.
+ Returntype : List of Bio::EnsEMBL::Mapper::Coordinate and/or
+ Bio::EnsEMBL::Mapper:Gap objects
+ Exceptions : thrown if if the specified TO coordinat system is not one
+ of the coordinate systems associated with this assembly mapper
Caller : general
=cut
+sub map {
+ throw('Incorrect number of arguments.') if(@_ != 6);
-sub map_coordinates_to_assembly {
- my ($self, $contig_id, $start, $end, $strand) = @_;
+ my ($self, $frm_seq_region, $frm_start, $frm_end, $frm_strand, $frm_cs) = @_;
+
+ my $mapper = $self->{'mapper'};
+ my $asm_cs = $self->{'asm_cs'};
+ my $cmp_cs = $self->{'cmp_cs'};
+ my $adaptor = $self->{'adaptor'};
+ my $frm;
+
+ #speed critical section:
+ #try to do simple pointer equality comparisons of the coord system objects
+ #first since this is likely to work most of the time and is much faster
+ #than a function call
+
+ if($frm_cs == $cmp_cs || ($frm_cs != $asm_cs && $frm_cs->equals($cmp_cs))) {
- if( ! exists $self->{'_contig_register'}->{$contig_id} ) {
- $self->register_region_around_contig( $contig_id, 0, 0 );
+ if(!$self->{'cmp_register'}->{$frm_seq_region}) {
+ $adaptor->register_component($self,$frm_seq_region);
}
+ $frm = $COMPONENT;
- return $self->{'_mapper'}->map_coordinates($contig_id, $start,
- $end, $strand, 'rawcontig');
+ } elsif($frm_cs == $asm_cs || $frm_cs->equals($asm_cs)) {
+
+ # This can be probably be sped up some by only calling registered
+ # assembled if needed
+ $adaptor->register_assembled($self,$frm_seq_region,$frm_start,$frm_end);
+ $frm = $ASSEMBLED;
+
+ } else {
+
+ throw("Coordinate system " . $frm_cs->name . " " . $frm_cs->version .
+ " is neither the assembled nor the component coordinate system " .
+ " of this AssemblyMapper");
+ }
+
+ return $mapper->map_coordinates($frm_seq_region, $frm_start, $frm_end,
+ $frm_strand, $frm);
}
-=head2 fast_to_assembly
- Arg 1 : int $contig_id
- raw contig internal ID
- Arg 2 : int $start
- start position on raw contig
- Arg 3 : int $end
- end position on raw contig
- Arg 4 : int $strand
- raw contig orientation (+/- 1)
+
+=head2 flush
+
+ Args : none
Example : none
- Description: takes RawContig coordinates and remaps it
- to Assembly coordinates. This is a fast simple version
- that only maps when there are no gaps and no splits.
- It will just return id, start, end, strand
- Returntype : list of results
- Exceptions : throws if args are not numeric
- Caller : general
+ Description: remove all cached items from this AssemblyMapper
+ Returntype : none
+ Exceptions : none
+ Caller : AssemblyMapperAdaptor
=cut
-sub fast_to_assembly {
- my ($self, $contig_id, $start, $end, $strand) = @_;
- if( ! exists $self->{'_contig_register'}->{$contig_id} ) {
- $self->register_region_around_contig( $contig_id, 0, 0 );
- }
- return $self->{'_mapper'}->fastmap($contig_id, $start, $end,
- $strand, 'rawcontig');
+sub flush {
+ my $self = shift;
+
+ $self->{'mapper'}->flush();
+ $self->{'cmp_register'} = {};
+ $self->{'asm_register'} = {};
}
-=head2 map_coordinates_to_rawcontig
+sub size {
+ my $self = shift;
+ return $self->{'mapper'}->{'pair_count'};
+}
- Arg 1 : string $chr_name
- Arg 2 : int $chr_start
- Arg 3 : int $chr_end
- Arg 4 : int $chr_strand
- From p to q is 1 reverse is -1
- Example : ( "X", 10000, 20000, -1 )
- Description: takes region in Assembly coordinates and
- remaps it to RawContig coordinates.
- Returntype : list of Bio::EnsEMBL::Mapper::Gap
- and/or Bio::EnsEMBL::Mapper::Coordinate
- The id method of each Coordinate object
- is the numeric contig_id of the RawContig
- it maps to
- Exceptions : argument type is checked where appropriat
- Caller : general
-=cut
+sub fastmap {
+ throw('Incorrect number of arguments.') if(@_ != 6);
-sub map_coordinates_to_rawcontig {
- my ($self, $chr_name, $start, $end, $strand) = @_;
-
- $self->register_region($chr_name, $start, $end);
-
- return $self->_mapper->map_coordinates($chr_name, $start, $end,
- $strand, 'assembly');
-}
+ my ($self, $frm_seq_region, $frm_start, $frm_end, $frm_strand, $frm_cs) = @_;
+ my $mapper = $self->{'mapper'};
+ my $asm_cs = $self->{'asm_cs'};
+ my $cmp_cs = $self->{'cmp_cs'};
+ my $adaptor = $self->{'adaptor'};
+ my $frm;
+ #speed critical section:
+ #try to do simple pointer equality comparisons of the coord system objects
+ #first since this is likely to work most of the time and is much faster
+ #than a function call
-=head2 list_contig_ids
+ if($frm_cs == $cmp_cs || ($frm_cs != $asm_cs && $frm_cs->equals($cmp_cs))) {
- Arg 1 : string $chr_name
- Arg 2 : int $chr_start
- Arg 3 : int $chr_end
- Example : ( "X", 1, 1000000 )
- Description: Returns a list of RawContig internal IDs
- which overlap the given chromosome region
- Returntype : list of int
- Exceptions : arguments are type checked
- Caller : general, used for SQL query generation
+ if(!$self->{'cmp_register'}->{$frm_seq_region}) {
+ $adaptor->register_component($self,$frm_seq_region);
+ }
+ $frm = $COMPONENT;
-=cut
+ } elsif($frm_cs == $asm_cs || $frm_cs->equals($asm_cs)) {
+ # This can be probably be sped up some by only calling registered
+ # assembled if needed
+ $adaptor->register_assembled($self,$frm_seq_region,$frm_start,$frm_end);
+ $frm = $ASSEMBLED;
-sub list_contig_ids {
- my ($self, $chr_name, $start, $end) = @_;
-
- # may not have registered this region yet
-
- $self->register_region($chr_name, $start, $end);
-
- return map
- { $_->from->id }
- $self->_mapper->list_pairs($chr_name, $start, $end, 'assembly');
+ } else {
+
+ throw("Coordinate system " . $frm_cs->name . " " . $frm_cs->version .
+ " is neither the assembled nor the component coordinate system " .
+ " of this AssemblyMapper");
+ }
+
+ return $mapper->fastmap($frm_seq_region, $frm_start, $frm_end,
+ $frm_strand, $frm);
}
-=head2 register_region
+=head2 list_seq_regions
- Arg 1 : string $chr_name
- Arg 2 : int $chr_start
- Arg 3 : int $chr_end
- Example : ( "X", 1, 1000000 )
- Description: Declares a chromosomal region to the AssemblyMapper.
- This extracts the relevant data from the assembly
- table and stores it in a Bio::EnsEMBL::Mapper.
- It therefore must be called before any mapping is
- attempted on that region. Otherwise only gaps will
- be returned! Is done automatically before mapping.
- Returntype : none
- Exceptions : arguments are checked
- Caller : internal
+ Arg [1] : string $frm_seq_region
+ The name of the sequence region of interest
+ Arg [2] : int $frm_start
+ The start of the region of interest
+ Arg [3] : int $frm_end
+ The end of the region to transform of interest
+ Arg [5] : Bio::EnsEMBL::CoordSystem $frm_cs
+ The coordinate system to obtain overlapping ids of
+ Example : foreach $id ($asm_mapper->list_ids('X',1,1000,$ctg_cs)) {...}
+ Description: Retrieves a list of overlapping seq_region internal identifiers
+ of another coordinate system. This is the same as the
+ list_ids method but uses seq_region names rather internal ids
+ Returntype : List of strings
+ Exceptions : none
+ Caller : general
=cut
-sub register_region {
- my ($self, $chr_name, $start, $end) = @_;
-
- my $first_chunk = int( $start / $self->_chunksize() );
- my $last_chunk = int( $end / $self->_chunksize() );
-
- $self->_chunk_register_region( $chr_name, $first_chunk, $last_chunk );
-}
+sub list_seq_regions {
+ throw('Incorrect number of arguments.') if(@_ != 5);
+ my($self, $frm_seq_region, $frm_start, $frm_end, $frm_cs) = @_;
+
+ if($frm_cs->equals($self->component_CoordSystem())) {
+ if(!$self->have_registered_component($frm_seq_region)) {
+ $self->adaptor->register_component($frm_seq_region);
+ }
+ #pull out the 'from' identifiers of the mapper pairs. The
+ #we loaded the assembled side as the 'from' side in the constructor
+ return
+ map {$_->from()->id()}
+ $self->mapper()->list_pairs($frm_seq_region, $frm_start,
+ $frm_end, $COMPONENT);
-=head2 register_region_around_contig
+ } elsif($frm_cs->equals($self->assembled_CoordSystem())) {
- Arg 1 : int $contig_id
- Arg 2 : int $upstream_bases
- Arg 3 : int $downstream_bases
- Example : ( 2000, 0, 0 )
- Description: Declares a region around a given RawContig to this
- AssemblyMapper. Is done automatically before a mapping
- is attempted. The registering is cached, so registering
- multiple times is cheap.
- With 0 as up and downstream tests if given contig is mappable.
- returns 1 if its is and 0 if it isnt.
- Returntype : int 0,1
- Exceptions : all Args must be ints
- Caller : internal
+ $self->adaptor->register_assembled($self,
+ $frm_seq_region,$frm_start,$frm_end);
+
+ #pull out the 'to' identifiers of the mapper pairs
+ #we loaded the component side as the 'to' coord system in the constructor
+ return
+ map {$_->to->id()}
+ $self->mapper()->list_pairs($frm_seq_region, $frm_start,
+ $frm_end, $ASSEMBLED);
+ } else {
+ throw("Coordinate system " . $frm_cs->name . " " . $frm_cs->version .
+ " is neither the assembled nor the component coordinate system " .
+ " of this AssemblyMapper");
+ }
+}
+
+
+=head2 list_ids
+
+ Arg [1] : string $frm_seq_region
+ The name of the sequence region of interest
+ Arg [2] : int $frm_start
+ The start of the region of interest
+ Arg [3] : int $frm_end
+ The end of the region to transform of interest
+ Arg [5] : Bio::EnsEMBL::CoordSystem $frm_cs
+ The coordinate system to obtain overlapping ids of
+ Example : foreach $id ($asm_mapper->list_ids('X',1,1000,$chr_cs)) {...}
+ Description: Retrieves a list of overlapping seq_region internal identifiers
+ of another coordinate system. This is the same as the
+ list_seq_regions method but uses internal identfiers rather
+ than seq_region strings
+ Returntype : List of ints
+ Exceptions : none
+ Caller : general
=cut
+sub list_ids {
+ throw('Incorrect number of arguments.') if(@_ != 5);
+ my($self, $frm_seq_region, $frm_start, $frm_end, $frm_cs) = @_;
+
+ #retrieve the seq_region names
+ my @seq_regs =
+ $self->list_seq_regions($frm_seq_region,$frm_start,$frm_end,$frm_cs);
+
+ #The seq_regions are from the 'to' coordinate system not the
+ #from coordinate system we used to obtain them
+ my $to_cs;
+ if($frm_cs->equals($self->assembled_CoordSystem())) {
+ $to_cs = $self->component_CoordSystem();
+ } else {
+ $to_cs = $self->assembled_CoordSystem();
+ }
-sub register_region_around_contig {
- my ($self, $contig_id, $left, $right) = @_;
-
- if( $self->_have_registered_contig( $contig_id )
- && $left == 0 && $right==0 ) {
- if( $self->_mapper->list_pairs( $contig_id, -1, -1, "rawcontig" )) {
- return 1;
- } else {
- return 0;
- }
- }
-
- my ( $chr_name, $chr_start, $chr_end ) =
- $self->adaptor()->register_contig( $self, $self->_type, $contig_id );
-
- if( defined $chr_name ) {
- $self->register_region( $chr_name, $chr_start-$left, $chr_end+$right );
- return 1;
- } else {
- return 0;
- }
+ #convert them to ids
+ return @{$self->adaptor()->seq_regions_to_ids($to_cs, \@seq_regs)};
}
-=head2 Internal functions
-Internal functions
+
+=head2 have_registered_component
+
+ Arg [1] : string $cmp_seq_region
+ The name of the sequence region to check for registration
+ Example : if($asm_mapper->have_registered_component('AL240214.1')) {...}
+ Description: Returns true if a given component region has been registered
+ with this assembly mapper. This should only be called
+ by this class or the AssemblyMapperAdaptor. Anotherwards, do
+ not use this method unless you really know what you are doing.
+ Returntype : 0 or 1
+ Exceptions : throw on incorrect arguments
+ Caller : internal, AssemblyMapperAdaptor
=cut
+sub have_registered_component {
+ my $self = shift;
+ my $cmp_seq_region = shift;
+ throw('cmp_seq_region argument is required') if(!$cmp_seq_region);
-=head2 _have_registered_contig
+ if($self->{'cmp_register'}->{$cmp_seq_region}) {
+ return 1;
+ }
- Arg 1 : int $rawContig_dbID
- Example : none
- Description: checks if given raw contig was registered before
- Returntype : int 0,1
- Exceptions : none
- Caller : internal
+ return 0;
+}
-=cut
+=head2 have_registered_assembled
-sub _have_registered_contig {
- my ($self,$id) = @_;
+ Arg [1] : string $asm_seq_region
+ The name of the sequence region to check for registration
+ Arg [2] : int $chunk_id
+ The chunk number of the provided seq_region to check for
+ registration.
+ Example : if($asm_mapper->have_registered_component('X',9)) {...}
+ Description: Returns true if a given assembled region chunk has been
+ registered with this assembly mapper. This should only
+ be called by this class or the AssemblyMapperAdaptor.
+ Anotherwards do not use this method unless you really know what
+ you are doing.
+ Returntype : 0 or 1
+ Exceptions : throw on incorrect arguments
+ Caller : internal, AssemblyMapperAdaptor
+
+=cut
- if( $self->{'_contig_register'}->{$id} ) {
- return 1;
- } else {
- return 0;
- }
+sub have_registered_assembled {
+ my $self = shift;
+ my $asm_seq_region = shift;
+ my $chunk_id = shift;
+ throw('asm_seq_region argument is required') if(!$asm_seq_region);
+ throw('chunk_id is required') if(!defined($chunk_id));
+
+ if($self->{'asm_register'}->{$asm_seq_region}->{$chunk_id}) {
+ return 1;
+ }
+
+ return 0;
}
-=head2 _register_contig
+=head2 register_component
- Arg 1 : int $rawContig_dbID
- Example : none
- Description: marks given raw contig as registered
+ Arg [1] : string $cmp_seq_region
+ The name of the component sequence region to register
+ Example : $asm_mapper->register_component('AL312341.1');
+ Description: Flags a given component sequence region as registered in this
+ assembly mapper. This should only be called by this class
+ or the AssemblyMapperAdaptor.
Returntype : none
- Exceptions : none
- Caller : internal
+ Exceptions : throw on incorrect arguments
+ Caller : internal, AssemblyMapperAdaptor
=cut
+sub register_component {
+ my $self = shift;
+ my $cmp_seq_region = shift;
+ throw('cmp_seq_region argument is required') if(!$cmp_seq_region);
-sub _register_contig {
- my ($self,$id) = @_;
-
- $self->{'_contig_register'}->{$id} = 1;
-
+ $self->{'cmp_register'}->{$cmp_seq_region} = 1;
}
+=head2 register_assembled
-=head2 _type
-
- Arg [1] : string $type
- Example : none
- Description: get/set of attribute _type
- Returntype : string
- Exceptions : none
- Caller : ?
+ Arg [1] : string $asm_seq_region
+ The name of the sequence region to register
+ Arg [2] : int $chunk_id
+ The chunk number of the provided seq_region to register.
+ Example : $asm_mapper->register_assembled('X', 4);
+ Description: Flags a given assembled region as registered in this assembly
+ mapper. This should only be called by this class or the
+ AssemblyMapperAdaptor. Do not call this method unless you
+ really know what you are doing.
+ Returntype : none
+ Exceptions : throw on incorrect arguments
+ Caller : internal, AssemblyMapperAdaptor
=cut
+sub register_assembled {
+ my $self = shift;
+ my $asm_seq_region = shift;
+ my $chunk_id = shift;
-sub _type {
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'_type'} = $value;
- }
- return $self->{'_type'};
+ throw('asm_seq_region argument is required') if(!$asm_seq_region);
+ throw('chunk_id srgument is required') if(!defined($chunk_id));
+ $self->{'asm_register'}->{$asm_seq_region}->{$chunk_id} = 1;
}
-=head2 _mapper
- Arg [1] : Bio::EnsEMBL::Mapper $mapper
- The mapper object which will be used to map between
- assembly and raw contigs
- Example : none
- Description: get/set of the attribute _mapper
+=head2 mapper
+
+ Arg [1] : none
+ Example : $mapper = $asm_mapper->mapper();
+ Description: Retrieves the internal mapper used by this Assembly Mapper.
+ This is unlikely to be useful unless you _really_ know what you
+ are doing.
Returntype : Bio::EnsEMBL::Mapper
Exceptions : none
- Caller : ?
+ Caller : internal, AssemblyMapperAdaptor
=cut
+sub mapper {
+ my $self = shift;
+ return $self->{'mapper'};
+}
-sub _mapper {
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'_mapper'} = $value;
- }
- return $self->{'_mapper'};
+=head2 assembled_CoordSystem
+
+ Arg [1] : none
+ Example : $cs = $asm_mapper->assembled_CoordSystem
+ Description: Retrieves the assembled CoordSystem from this assembly mapper
+ Returntype : Bio::EnsEMBL::CoordSystem
+ Exceptions : none
+ Caller : internal, AssemblyMapperAdaptor
+
+=cut
+
+sub assembled_CoordSystem {
+ my $self = shift;
+ return $self->{'asm_cs'};
}
+=head2 component_CoordSystem
+
+ Arg [1] : none
+ Example : $cs = $asm_mapper->component_CoordSystem
+ Description: Retrieves the component CoordSystem from this assembly mapper
+ Returntype : Bio::EnsEMBL::CoordSystem
+ Exceptions : none
+ Caller : internal, AssemblyMapperAdaptor
+
+=cut
+
+sub component_CoordSystem {
+ my $self = shift;
+ return $self->{'cmp_cs'};
+}
+
=head2 adaptor
@@ -415,119 +534,102 @@ sub _mapper {
sub adaptor {
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'adaptor'} = $value;
- }
- return $self->{'adaptor'};
-
+ my $self = shift;
+ $self->{'adaptor'} = shift if(@_);
+ return $self->{'adaptor'};
}
-# this function should be customized for the assemblies that are in
-# EnsEMBL. (Could be a hash of assembly_type ->size )
+=head2 in_assembly
+
+ Description: Deprecated. Use map() or list_ids() instead
+=cut
-=head2 _chunksize
+sub in_assembly {
+ my ($self, $object) = @_;
- Args : none
- Example : none
- Description: returns the size in which this mapper registers chromosomal
- regions. It should be optimized on the size of pieces
- in assembly table, so that not too many and not too little
- rows are retrieved.
- Returntype : int
- Exceptions : none
- Caller : internal
+ deprecate('Use map() or list_ids() instead.');
-=cut
+ my $csa = $self->db->get_CoordSystemAdaptor();
+
+ my $top_level = $csa->fetch_top_level();
+
+ my $asma = $self->adaptor->fetch_by_CoordSystems($object->coord_system(),
+ $top_level);
+ my @list = $asma->list_ids($object->seq_region(), $object->start(),
+ $object->end(), $object->coord_system());
-sub _chunksize {
- return 1000000;
+ return (@list > 0);
}
+=head2 map_coordinates_to_assembly
-=head2 _chunk_register_region
+ Description: DEPRECATED use map() instead
- Arg 1 : string $chr_name
- Arg 2 : int $first_chunk
- Arg 3 : int $last_chunk
- Example : none
- Description: registers the region on given chromosome from
- $first_chunk*chunksize until ($last_chunk+1)*chunksize-1
- Returntype : none
- Exceptions : none
- Caller : register_region
+=cut
+
+sub map_coordinates_to_assembly {
+ my ($self, $contig_id, $start, $end, $strand) = @_;
+
+ deprecate('Use map() instead.');
+
+ #not sure if contig_id is seq_region_id or name...
+ return $self->map($contig_id, $start, $end, $strand,
+ $self->contig_CoordSystem());
+
+}
+
+
+=head2 fast_to_assembly
+
+ Description: DEPRECATED use map() instead
=cut
+sub fast_to_assembly {
+ my ($self, $contig_id, $start, $end, $strand) = @_;
-sub _chunk_register_region {
- my ( $self, $chr_name, $first_chunk, $last_chunk ) = @_;
+ deprecate('Use map() instead.');
- foreach my $i ( $first_chunk..$last_chunk ) {
- if( exists $self->{$chr_name}{$i} ) {
- next;
- } else {
- $self->{$chr_name}{$i} = 1;
- my $start = $i * $self->_chunksize();
- my $end = $start + $self->_chunksize() - 1;
- $self->adaptor->register_region
- ( $self, $self->_type, $chr_name, $start, $end);
-
- }
- }
+ #not sure if contig_id is seq_region_id or name...
+ return $self->map($contig_id, $start, $end, $strand,
+ $self->contig_CoordSystem());
}
-=head2 in_assembly
+=head2 map_coordinates_to_rawcontig
- Arg 1 : Bio::EnsEMBL::Clone or
- Bio::EnsEMBL::RawContig $object_in_assembly
-
- Example : none
- Description: tests if the given Clone or RawContig object is in the
- assembly
- Returntype : int 0,1
- Exceptions : argument type is checked
- Caller : general
+ Description: DEPRECATED use map() instead
=cut
+sub map_coordinates_to_rawcontig {
+ my ($self, $chr_name, $start, $end, $strand) = @_;
-sub in_assembly {
- my ($self, $object) = @_;
+ deprecate('Use map() instead.');
- my @contigs;
+ return $self->map($chr_name, $start, $end, $strand,
+ $self->assembled_CoordSystem());
- unless(ref $object) {
- $self->throw("$object is not an object reference");
- }
+}
- if($object->isa("Bio::EnsEMBL::Clone")) {
- #get contigs from this clone
- @contigs = @{$object->get_all_Contigs()};
- } elsif ($object->isa("Bio::EnsEMBL::RawContig")) {
- #we already have the contig we need
- @contigs = ($object);
- } else {
- #object is not a clone or a raw contig
- $self->throw("$object is not a RawContig or Clone object");
- }
+=head2 list_contig_ids
+ Description: DEPRECATED Use list_ids instead
- #verify at least one of these contigs is mapped to the assembly
- foreach my $contig (@contigs) {
- if($self->register_region_around_contig( $contig->dbID(),
- 0, 0)) {
- return 1;
- }
- }
+=cut
- #none of the contigs was in the assembly (golden path)
- return 0;
+sub list_contig_ids {
+ my ($self, $chr_name, $start, $end) = @_;
+
+ deprecate('Use list_ids() instead.');
+
+ return $self->list_ids($chr_name, $start, $end,
+ $self->assembled_CoordSystem());
}
+
1;
diff --git a/modules/Bio/EnsEMBL/BaseAlignFeature.pm b/modules/Bio/EnsEMBL/BaseAlignFeature.pm
index 9b10abb301..59d78d104b 100644
--- a/modules/Bio/EnsEMBL/BaseAlignFeature.pm
+++ b/modules/Bio/EnsEMBL/BaseAlignFeature.pm
@@ -1,6 +1,5 @@
# EnsEMBL module for storing dna-protein pairwise alignments
#
-# Cared for by Michele Clamp <michele@sanger.ac.uk>
#
# You may distribute this module under the same terms as perl itself
#
@@ -8,19 +7,21 @@
=head1 NAME
-Bio::EnsEMBL::DnaPepAlignFeature - Ensembl specific dna-protein
- pairwise alignment feature
+Bio::EnsEMBL::BaseAlignFeature - Baseclass providing a common abstract
+implmentation for alignment features
=head1 SYNOPSIS
- my $feat = new Bio::EnsEMBL::DnaPepAlignFeature(-seqname => 'myseq',
- -start => 100,
- -end => 120,
- -strand => 1,
- -hstart => 200,
- -hend => 220,
- -analysis => $analysis,
- -cigar_string => '');
+ my $feat = new Bio::EnsEMBL::DnaPepAlignFeature
+ (-slice => $slice,
+ -start => 100,
+ -end => 120,
+ -strand => 1,
+ -hseqname => 'SP:RF1231',
+ -hstart => 200,
+ -hend => 220,
+ -analysis => $analysis,
+ -cigar_string => '10M3D5M2I');
Alternatively if you have an array of ungapped features
@@ -35,13 +36,13 @@ Bio::EnsEMBL::DnaPepAlignFeature - Ensembl specific dna-protein
This converts the cigar string into an array of Bio::EnsEMBL::FeaturePair
$analysis is a Bio::EnsEMBL::Analysis object
-
- Bio::EnsEMBL::SeqFeature methods can be used
+
+ Bio::EnsEMBL::Feature methods can be used
Bio::EnsEMBL::FeaturePair methods can be used
The cigar_string contains the ungapped pieces that make up the gapped
alignment
-
+
It looks like: n Matches [ x Deletes or Inserts m Matches ]*
but a bit more condensed like "23M4I12M2D1M"
and evenmore condensed as you can ommit 1s "23M4I12M2DM"
@@ -77,14 +78,15 @@ Bio::EnsEMBL::DnaPepAlignFeature - Ensembl specific dna-protein
These ungapped pieces are made up into the following string (called a cigar
string) "33M3I27M3I137M" with start 267 end 479 strand -1 hstart 7 hend 75
hstrand 1 and feature type would be DnaPepAlignFeature
-
-=cut
+
+=cut
package Bio::EnsEMBL::BaseAlignFeature;
use Bio::EnsEMBL::FeaturePair;
-use Bio::EnsEMBL::SeqFeature;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
use vars qw(@ISA);
use strict;
@@ -111,24 +113,25 @@ use strict;
=cut
sub new {
- my ($class,@args) = @_;
-
- my $self = $class->SUPER::new(@args);
- #print "calling new with @args\n";
- my ($cigar_string,$features)
- = $self->_rearrange([qw(CIGAR_STRING FEATURES)], @args);
-
- if (defined($cigar_string) && defined($features)) {
- $self->throw("Can't input cigar_string and an array of features.");
- } elsif (defined($features)) {
- $self->_parse_features($features);
- } elsif (!defined($cigar_string)) {
- $self->throw("Must have a cigar string defining the alignment");
- }
-
- $self->cigar_string($cigar_string);
-
- return $self;
+ my $caller = shift;
+
+ my $class = ref($caller) || $caller;
+
+ my $self = $class->SUPER::new(@_);
+
+ my ($cigar_string,$features) = rearrange([qw(CIGAR_STRING FEATURES)], @_);
+
+ if (defined($cigar_string) && defined($features)) {
+ throw("CIGAR_STRING or FEATURES argument is required - not both.");
+ } elsif (defined($features)) {
+ $self->_parse_features($features);
+ } elsif (defined($cigar_string)) {
+ $self->{'cigar_string'} = $cigar_string;
+ } else {
+ throw("CIGAR_STRING or FEATURES argument is required");
+ }
+
+ return $self;
}
@@ -156,6 +159,7 @@ sub new_fast {
return bless $hashref, $class;
}
+
=head2 cigar_string
Arg [1] : string $cigar_string
@@ -166,62 +170,18 @@ sub new_fast {
(thats the ensembl sequence), "xD" for deletions
(inserts in the subject). an "x" that is 1 can be omitted.
Returntype : string
- Exceptions : throws on a get without previous set
+ Exceptions : none
Caller : general
=cut
sub cigar_string {
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
-
- # Do some checks to see whether this is valid
- my $tmp = $arg;
-
- $self->{_cigar_string} = $arg;
- }
-
- if (!defined($self->{_cigar_string})) {
- $self->throw("No cigar string defined - can't return one");
- }
-
- return $self->{_cigar_string};
+ my $self = shift;
+ $self->{'cigar_string'} = shift if(@_);
+ return $self->{'cigar_string'};
}
-=head2 alignment_length
-
- Arg [1] : None
- Example :
- Description: return the alignment length (including indels) based on the cigar_string
- Returntype : int
- Exceptions :
- Caller :
-
-=cut
-
-sub alignment_length {
- my $self = shift;
-
- if (! defined $self->{'_alignment_length'} && defined $self->cigar_string) {
-
- my @pieces = ( $self->cigar_string =~ /(\d*[MDI])/g );
- unless (@pieces) {
- print STDERR "Error parsing cigar_string\n";
- }
- my $alignment_length = 0;
- foreach my $piece (@pieces) {
- my ($length) = ( $piece =~ /^(\d*)/ );
- if (! defined $length || $length eq "") {
- $length = 1;
- }
- $alignment_length += $length;
- }
- $self->{'_alignment_length'} = $alignment_length;
- }
- return $self->{'_alignment_length'};
-}
=head2 ungapped_features
@@ -238,60 +198,11 @@ sub alignment_length {
sub ungapped_features {
my ($self) = @_;
- if (defined($self->cigar_string)) {
- my @features = $self->_parse_cigar;
- return @features;
- } else {
- $self->throw("No cigar_string defined. Can't return ungapped features");
- }
-
-}
-
-
-
-=head2 dbID
-
- Arg [1] : int $dbID
- Example : none
- Description: get/set for the database internal id
- Returntype : int
- Exceptions : none
- Caller : general, set from adaptor on store or retrieval
-
-=cut
-
-sub dbID{
- my ($self, $arg) = @_;
-
- if(defined $arg){
- $self->{_database_id} = $arg;
+ if (!defined($self->{'cigar_string'})) {
+ throw("No cigar_string defined. Can't return ungapped features");
}
- return $self->{_database_id};
-
-}
-
-
-
-=head2 adaptor
-
- Arg [1] : Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor $adaptor
- Example : none
- Description: get/set for this objects Adaptor
- Returntype : Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub adaptor {
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'adaptor'} = $value;
- }
- return $self->{'adaptor'};
-
+ return @{$self->_parse_cigar()};
}
@@ -314,7 +225,7 @@ sub reverse_complement {
# reverse strand in both sequences
$self->strand($self->strand * -1);
$self->hstrand($self->hstrand * -1);
-
+
# reverse cigar_string as consequence
my $cigar_string = $self->cigar_string;
$cigar_string =~ s/(D|I|M)/$1 /g;
@@ -347,36 +258,34 @@ sub _parse_cigar {
my $query_unit = $self->_query_unit();
my $hit_unit = $self->_hit_unit();
-
- my $string = $self->cigar_string;
-
- unless(defined($string)) {
- $self->throw("No cigar string defined in object. This should be caught" .
- "by the cigar_string method and never happen");
- }
+ my $string = $self->{'cigar_string'};
+
+ throw("No cigar string defined in object") if(!defined($string));
my @pieces = ( $string =~ /(\d*[MDI])/g );
#print "cigar: ",join ( ",", @pieces ),"\n";
-
+
my @features;
- my $strand1 = $self->strand() || 1;
- my $strand2 = $self->hstrand() || 1;
+ my $strand1 = $self->{'strand'} || 1;
+ my $strand2 = $self->{'hstrand'}|| 1;
my ( $start1, $start2 );
-
+
if( $strand1 == 1 ) {
- $start1 = $self->start();
+ $start1 = $self->{'start'};
} else {
- $start1 = $self->end();
+ $start1 = $self->{'end'};
}
if( $strand2 == 1 ) {
- $start2 = $self->hstart();
+ $start2 = $self->{'hstart'};
} else {
- $start2 = $self->hend();
+ $start2 = $self->{'hend'};
}
-
+ #
+ # Construct ungapped blocks as FeaturePairs objects for each MATCH
+ #
foreach my $piece (@pieces) {
my ($length) = ( $piece =~ /^(\d*)/ );
@@ -392,78 +301,80 @@ sub _parse_cigar {
} elsif ( $query_unit == 1 && $hit_unit == 1 ) {
$mapped_length = $length;
} else {
- $self->throw("Internal error $query_unit $hit_unit, currently only " .
- "allowing 1 or 3 ");
+ throw("Internal error $query_unit $hit_unit, currently only " .
+ "allowing 1 or 3 ");
}
-
+
if( int($mapped_length) != $mapped_length ) {
- $self->throw("Internal error with mismapped length of hit, query " .
- "$query_unit, hit $hit_unit, length $length");
+ throw("Internal error with mismapped length of hit, query " .
+ "$query_unit, hit $hit_unit, length $length");
}
if( $piece =~ /M$/ ) {
- my $fp = new Bio::EnsEMBL::FeaturePair;
-
- my ( $a, $b );
+ #
+ # MATCH
+ #
+ my ( $qstart, $qend);
if( $strand1 == 1 ) {
- $a = $start1;
- $b = $start1 + $length - 1;
- $start1 = $b + 1;
+ $qstart = $start1;
+ $qend = $start1 + $length - 1;
+ $start1 = $qend + 1;
} else {
- $b = $start1;
- $a = $start1 - $length + 1;
- $start1 = $a - 1;
+ $qend = $start1;
+ $qstart = $start1 - $length + 1;
+ $start1 = $qstart - 1;
}
-
- $fp->start($a);
- $fp->end ($b);
- $fp->strand($self->strand() );
- $fp->score($self->score);
- $fp->seqname($self->seqname);
- $fp->phase($self->phase);
- $fp->p_value($self->p_value);
- $fp->percent_id($self->percent_id);
+ my ($hstart, $hend);
if( $strand2 == 1 ) {
- $a = $start2;
- $b = $start2 + $mapped_length - 1;
- $start2 = $b + 1;
+ $hstart = $start2;
+ $hend = $start2 + $mapped_length - 1;
+ $start2 = $hend + 1;
} else {
- $b = $start2;
- $a = $start2 - $mapped_length + 1;
- $start2 = $a - 1;
+ $hend = $start2;
+ $hstart = $start2 - $mapped_length + 1;
+ $start2 = $hstart - 1;
}
- $fp->hstart($a);
- $fp->hend($b);
- $fp->hstrand($self->hstrand());
- $fp->hseqname($self->hseqname);
-
- $fp->contig($self->contig);
- $fp->analysis($self->analysis);
+ push @features, Bio::EnsEMBL::FeaturePair->new
+ (-SLICE => $self->{'slice'},
+ -START => $qstart,
+ -END => $qend,
+ -STRAND => $strand1,
+ -HSEQNAME => $self->{'hseqname'},
+ -HSTART => $hstart,
+ -HEND => $hend,
+ -HSTRAND => $strand2,
+ -SCORE => $self->{'score'},
+ -PERCENT_ID => $self->{'percent_id'},
+ -ANALYSIS => $self->{'analysis'},
+ -P_VALUE => $self->{'p_value'});
- push(@features,$fp);
# end M cigar bits
} elsif( $piece =~ /I$/ ) {
+ #
+ # INSERT
+ #
if( $strand1 == 1 ) {
$start1 += $length;
} else {
$start1 -= $length;
}
} elsif( $piece =~ /D$/ ) {
+ #
+ # DELETION
+ #
if( $strand2 == 1 ) {
$start2 += $mapped_length;
} else {
$start2 -= $mapped_length;
}
} else {
- $self->throw( "Illegal cigar line $string!" );
- }
-
+ throw( "Illegal cigar line $string!" );
+ }
}
- # should the features be sorted ?
- #
- return @features;
+
+ return \@features;
}
@@ -477,7 +388,7 @@ sub _parse_cigar {
entries.
Returntype : none, fills in values of self
Exceptions : argument list undergoes many sanity checks - throws under many
- invalid conditions
+ invalid conditions
Caller : new
=cut
@@ -489,13 +400,15 @@ sub _parse_features {
my $hit_unit = $self->_hit_unit();
if (ref($features) ne "ARRAY") {
- $self->throw("features must be an array reference not a [" .
- ref($features) . "]");
+ throw("features must be an array reference not a [".ref($features)."]");
}
- my $strand = $features->[0]->strand;
+ my $strand = $features->[0]->strand;
+
+ throw ('FeaturePair needs to have strand == 1 or strand == -1') if(!$strand);
+
my @f;
-
+
#
# Sort the features on their start position
# Ascending order on positive strand, descending on negative strand
@@ -506,30 +419,25 @@ sub _parse_features {
@f = sort { $b->start <=> $a->start} @$features;
}
-
my $hstrand = $f[0]->hstrand;
- my $contig = $f[0]->contig();
- my $name = $f[0]->seqname;
+ my $slice = $f[0]->slice();
+ my $name = $slice->name() if($slice);
my $hname = $f[0]->hseqname;
my $score = $f[0]->score;
my $percent = $f[0]->percent_id;
- my $pvalue = $f[0]->p_value;
my $analysis = $f[0]->analysis;
- my $phase = $f[0]->phase;
-
+ my $pvalue = $f[0]->p_value();
+
# implicit strand 1 for peptide sequences
- ( defined $strand ) || ( $strand = 1 );
- ( defined $hstrand ) || ( $hstrand = 1 );
+ $strand ||= 1;
+ $hstrand ||= 1;
my $ori = $strand * $hstrand;
-
-
- if (scalar(@f) == 0) {
- $self->throw("No features in the array to parse");
- }
+
+ throw("No features in the array to parse") if(scalar(@f) == 0);
my $prev1; # where last feature q part ended
my $prev2; # where last feature s part ended
-
+
my $string;
# Use strandedness info of query and hit to make sure both sets of
@@ -565,35 +473,35 @@ sub _parse_features {
#
if (!$f->isa("Bio::EnsEMBL::FeaturePair")) {
- $self->throw("Array element [$f] is not a Bio::EnsEMBL::FeaturePair");
+ throw("Array element [$f] is not a Bio::EnsEMBL::FeaturePair");
}
- if( defined $f->hstrand() ) {
- if ($f->hstrand != $hstrand) {
- $self->throw("Inconsistent hstrands in feature array");
- }
+ if( defined($f->hstrand()) && $f->hstrand() != $hstrand ) {
+ throw("Inconsistent hstrands in feature array");
}
- if( defined $f->strand() ) {
- if ($f->strand != $strand) {
- $self->throw("Inconsistent strands in feature array");
- }
+ if( defined($f->strand()) && ($f->strand != $strand)) {
+ throw("Inconsistent strands in feature array");
}
- if ($name ne $f->seqname) {
- $self->throw("Inconsistent names in feature array [$name - " .
- $f->seqname . "]");
+ if ( defined($name) && $name ne $f->slice->name()) {
+ throw("Inconsistent names in feature array [$name - ".
+ $f->slice->name()."]");
}
- if ($hname ne $f->hseqname) {
- $self->throw("Inconsistent names in feature array [$hname - " .
- $f->hseqname . "]");
+ if ( defined($hname) && $hname ne $f->hseqname) {
+ throw("Inconsistent hit names in feature array [$hname - ".
+ $f->hseqname . "]");
}
- if ($score ne $f->score) {
- $self->throw("Inconsisent scores in feature array [$score - " .
- $f->score . "]");
+ if ( defined($score) && $score ne $f->score) {
+ throw("Inconsisent scores in feature array [$score - " .
+ $f->score . "]");
}
if (defined($f->percent_id) && $percent ne $f->percent_id) {
- $self->throw("Inconsistent pids in feature array [$percent - " .
- $f->percent_id . "]");
+ throw("Inconsistent pids in feature array [$percent - " .
+ $f->percent_id . "]");
+ }
+ if(defined($pvalue) && $pvalue != $f->p_value()) {
+ throw("Inconsistant p_values in feature arraw [$pvalue " .
+ $f->p_value() . "]");
}
-
+
my $start1 = $f->start; #source sequence alignment start
my $start2 = $f->hstart(); #hit sequence alignment start
@@ -602,18 +510,17 @@ sub _parse_features {
#
if (defined($prev1)) {
if ( $strand == 1 ) {
- if ($f->start < $prev1) {
- $self->throw("Inconsistent coordinates feature is forward strand " .
- "hstart in current feature should be greater than " .
- "hend in previous feature " . $f->start . " < " .
- $prev1."\n");
- }
+ if ($f->start < $prev1) {
+ throw("Inconsistent coords in feature array (forward strand).\n" .
+ "Start [".$f->start()."] in current feature should be greater " .
+ "than previous feature end [$prev1].");
+ }
} else {
- if ($f->end > $prev1) {
- $self->throw("Inconsistent coordinates in feature array feature " .
- "is reverse strand hend should be less than previous " .
- "hstart " . $f->end . " > $prev1");
- }
+ if ($f->end > $prev1) {
+ throw("Inconsistent coords in feature array (reverse strand).\n" .
+ "End [".$f->end() ."] should be less than previous feature " .
+ "start [$prev1].");
+ }
}
}
@@ -622,7 +529,7 @@ sub _parse_features {
# using multiplication to avoid rounding errors, hence the
# switch from query to hit for the ratios
-
+
#
# Yet more sanity checking
#
@@ -632,19 +539,17 @@ sub _parse_features {
my $query_p_length = sprintf "%.0f", ($length/$query_unit);
my $hit_p_length = sprintf "%.0f", ($hlength * $hit_unit);
if( $query_p_length != $hit_p_length) {
- #print STDERR $length."/".$query_unit." ".$hlength."*".$hit_unit."\n";
- $self->throw( "Feature lengths not comparable Lengths:" .$length .
- " " . $hlength . " Ratios:" . $query_unit . " " .
- $hit_unit );
+ throw( "Feature lengths not comparable Lengths:" .$length .
+ " " . $hlength . " Ratios:" . $query_unit . " " .
+ $hit_unit );
}
} else{
my $query_d_length = sprintf "%.0f", ($length*$hit_unit);
my $hit_d_length = sprintf "%.0f", ($hlength * $query_unit);
if( $length * $hit_unit != $hlength * $query_unit ) {
-
- $self->throw( "Feature lengths not comparable Lengths:" . $length .
- " " . $hlength . " Ratios:" . $query_unit . " " .
- $hit_unit );
+ throw( "Feature lengths not comparable Lengths:" . $length .
+ " " . $hlength . " Ratios:" . $query_unit . " " .
+ $hit_unit );
}
}
@@ -660,13 +565,13 @@ sub _parse_features {
if( $strand == 1 ) {
if( ( defined $prev1 ) && ( $f->start > $prev1 + 1 )) {
- #there is an insertion
- $insertion_flag = 1;
- my $gap = $f->start - $prev1 - 1;
- if( $gap == 1 ) {
- $gap = ""; # no need for a number if gap length is 1
- }
- $string .= "$gap"."I";
+ #there is an insertion
+ $insertion_flag = 1;
+ my $gap = $f->start - $prev1 - 1;
+ if( $gap == 1 ) {
+ $gap = ""; # no need for a number if gap length is 1
+ }
+ $string .= "$gap"."I";
}
@@ -676,19 +581,19 @@ sub _parse_features {
if(( defined $prev1 ) && ($f->end + 1 < $prev1 )) {
- #there is an insertion
- $insertion_flag = 1;
- my $gap = $prev1 - $f->end() - 1;
- if( $gap == 1 ) {
- $gap = ""; # no need for a number if gap length is 1
- }
- $string .= "$gap"."I";
+ #there is an insertion
+ $insertion_flag = 1;
+ my $gap = $prev1 - $f->end() - 1;
+ if( $gap == 1 ) {
+ $gap = ""; # no need for a number if gap length is 1
+ }
+ $string .= "$gap"."I";
}
#shift our position in the source seq alignment
$prev1 = $f->start();
}
-
+
#
# Check to see if there is a D type (deletion) gap
# There is a deletion gap if there is a space between the end of the
@@ -697,48 +602,47 @@ sub _parse_features {
if( $hstrand == 1 ) {
if(( defined $prev2 ) && ( $f->hstart() > $prev2 + 1 )) {
- #there is a deletion
- my $gap = $f->hstart - $prev2 - 1;
- my $gap2 = int( $gap * $hlengthfactor + 0.05 );
+ #there is a deletion
+ my $gap = $f->hstart - $prev2 - 1;
+ my $gap2 = int( $gap * $hlengthfactor + 0.5 );
- if( $gap2 == 1 ) {
- $gap2 = ""; # no need for a number if gap length is 1
- }
- $string .= "$gap2"."D";
-
- #sanity check, Should not be an insertion and deletion
- if($insertion_flag) {
- $self->warn("Should not be an deletion and insertion on the " .
- "same alignment region. cigar_line=$string\n");
- }
- }
+ if( $gap2 == 1 ) {
+ $gap2 = ""; # no need for a number if gap length is 1
+ }
+ $string .= "$gap2"."D";
+
+ #sanity check, Should not be an insertion and deletion
+ if($insertion_flag) {
+ warning("Should not be an deletion and insertion on the " .
+ "same alignment region. cigar_line=$string\n");
+ }
+ }
#shift our position in the hit seq alignment
$prev2 = $f->hend();
} else {
if( ( defined $prev2 ) && ( $f->hend() + 1 < $prev2 )) {
- #there is a deletion
- my $gap = $prev2 - $f->hend - 1;
- my $gap2 = int( $gap * $hlengthfactor + 0.05 );
+ #there is a deletion
+ my $gap = $prev2 - $f->hend - 1;
+ my $gap2 = int( $gap * $hlengthfactor + 0.5 );
- if( $gap2 == 1 ) {
- $gap2 = ""; # no need for a number if gap length is 1
- }
- $string .= "$gap2"."D";
-
- #sanity check, Should not be an insertion and deletion
- if($insertion_flag) {
- $self->throw("Should not be an deletion and insertion on the " .
- "same alignment region. prev2 = $prev2; f->hend() = " .
- $f->hend() . "; cigar_line = $string;\n");
- }
+ if( $gap2 == 1 ) {
+ $gap2 = ""; # no need for a number if gap length is 1
+ }
+ $string .= "$gap2"."D";
+
+ #sanity check, Should not be an insertion and deletion
+ if($insertion_flag) {
+ throw("Should not be an deletion and insertion on the " .
+ "same alignment region. prev2 = $prev2; f->hend() = " .
+ $f->hend() . "; cigar_line = $string;\n");
+ }
}
#shift our position in the hit seq alignment
-
$prev2 = $f->hstart();
}
-
+
my $matchlength = $f->end() - $f->start() + 1;
if( $matchlength == 1 ) {
$matchlength = "";
@@ -746,117 +650,23 @@ sub _parse_features {
$string .= $matchlength."M";
}
- if(!$score){
- #$self->warn("score is not set assume its 1");
- $score = 1;
- }
- my $feature1 = new Bio::EnsEMBL::SeqFeature();
-
- $feature1->start($f1start);
- $feature1->end ($f1end);
- $feature1->strand($strand);
- $feature1->score($score);
- $feature1->percent_id($percent);
- $feature1->p_value($pvalue);
- if( $contig ) {
- $feature1->contig($contig);
- } else {
- $feature1->seqname($name);
- }
- $feature1->phase($phase);
- $feature1->analysis($analysis);
- $feature1->validate;
-
- my $feature2 = new Bio::EnsEMBL::SeqFeature();
-
- $feature2->start($f2start);
- $feature2->end ($f2end);
- $feature2->strand($hstrand);
- $feature2->score($score);
- $feature2->seqname($hname);
- $feature2->percent_id($percent);
- $feature2->p_value($pvalue);
- $feature2->phase($phase);
- $feature2->analysis($analysis);
- $feature2->validate;
-
-
- $self->feature1($feature1);
- $self->feature2($feature2);
- $self->cigar_string($string);
-
+ $self->{'start'} = $f1start;
+ $self->{'end'} = $f1end;
+ $self->{'strand'} = $strand;
+ $self->{'score'} = $score;
+ $self->{'percent_id'} = $percent;
+ $self->{'analysis'} = $analysis;
+ $self->{'slice'} = $slice;
+ $self->{'hstart'} = $f2start;
+ $self->{'hend'} = $f2end;
+ $self->{'hstrand'} = $hstrand;
+ $self->{'hseqname'} = $hname;
+ $self->{'cigar_string'} = $string;
+ $self->{'p_value'} = $pvalue;
}
-=head2 _transform_to_RawContig
-
- Arg [1] : none
- Example : none
- Description: PRIVATE method. Transforms this feature from slice coordinates
- to rawcontig coodinates.
- Returntype : list of BaseAlignFeatures
- Exceptions : none
- Caller : transform
-
-=cut
-
-sub _transform_to_RawContig{
- my ($self) = @_;
-
- my $slice = $self->contig;
-
- unless($slice){
- $self->throw("can't transform coordinates of $self "
- . " without slice attached to feature");
- }
-
- my $adaptor = $slice->adaptor;
-
- unless($adaptor) {
- $self->throw("can't transform $self without an adaptor attached " .
- "to the feature's slice");
- }
-
- my $rcAdaptor = $adaptor->db->get_RawContigAdaptor();
- my @out;
-
- #parse cigarline and split this gapped feature into list of ungapped features
- my @features = $self->_parse_cigar();
-
- my @mapped_features;
- my %rc_features;
-
- #transform each of the ungapped features into raw contig coordinates
- foreach my $f(@features){
- push @mapped_features, $self->_transform_feature_to_RawContig($f);
- }
-
- #sort the transformed ungapped features into contig buckets
- foreach my $mf(@mapped_features){
- my $contig_id = $mf->contig->dbID;
- unless($rc_features{$contig_id}){
- $rc_features{$contig_id} = [];
- }
- push(@{$rc_features{$contig_id}}, $mf);
- }
-
- #create a single gapped feature from all the ungapped features
- #in each contig bucket
- foreach my $contig_id (keys(%rc_features)){
- #create a gapped feature from a list of ungapped features
- my $outputf = $self->new( -features => $rc_features{$contig_id} );
- $outputf->analysis( $self->analysis() );
- $outputf->score( $self->score() );
- $outputf->percent_id( $self->percent_id() );
- $outputf->p_value( $self->p_value());
- my $contig = $rcAdaptor->fetch_by_dbID($contig_id);
- $outputf->contig($contig);
- push(@out, $outputf);
- }
-
- return @out;
-}
@@ -874,7 +684,7 @@ sub _transform_to_RawContig{
sub _hit_unit {
my $self = shift;
- $self->throw( "Abstract method call!" );
+ throw( "Abstract method call!" );
}
@@ -893,221 +703,10 @@ sub _hit_unit {
sub _query_unit {
my $self = shift;
- $self->throw( "Abstract method call!" );
+ throw( "Abstract method call!" );
}
-=head2 _transform_feature_to_RawContig
-
- Arg [1] : Bio::EnsEMBL::FeaturePair
- Example : none
- Description: Transforms a component ungapped feature from slice coordinates
- to RawContig coordinates.
- Returntype : list of Bio::EnsEMBL::FeaturePairs
- Exceptions : thrown if feature is not attached to a contig (slice)
- thrown if attached slice does not have an adaptor
- Caller : internal
-
-=cut
-
-sub _transform_feature_to_RawContig{
- my($self, $feature) = @_;
-
- my $slice = $feature->contig;
-
- unless($slice){
- $self->throw("can't transform coordinates of ".$self.
- " without some sort of contig defined");
- }
-
- my $adaptor = $slice->adaptor;
-
- unless($adaptor) {
- $self->throw("can't tranform coordinates of $self without " .
- "adaptor attached to feature's slice");
- }
-
- my $asma = $adaptor->db->get_AssemblyMapperAdaptor;
- my $mapper = $asma->fetch_by_type( $self->contig()->assembly_type() );
-
- my $rcAdaptor = $adaptor->db->get_RawContigAdaptor();
-
- my $slice_start = $slice->chr_start;
- my $slice_end = $slice->chr_end;
- my $slice_strand = $slice->strand;
- my ($global_start, $global_end, $global_strand);
-
- #change feature coords from slice coordinates to assembly coords
- if($slice_strand == 1) {
- $global_start = $feature->start + $slice_start - 1;
- $global_end = $feature->end + $slice_start - 1;
- $global_strand = $feature->strand;
- } else {
- $global_start = $slice_end - $feature->end + 1;
- $global_end = $slice_end - $feature->start + 1;
- $global_strand = $feature->strand * -1;
- }
-
-
- #convert assembly coords to raw contig coords
- my @mapped = $mapper->map_coordinates_to_rawcontig
- (
- $slice->chr_name(),
- $global_start,
- $global_end,
- $global_strand
- );
-
- if( ! @mapped ) {
- $self->throw( "couldn't map ".$self."\n" );
- return $self;
- }
-
- my @out;
- my ( $hit_start, $hit_end );
- my $codon_unused_bases = 0;
-
- if( scalar( @mapped ) > 1 ) {
- #The feature needs to be mapped accross multiple contigs
-
- if( $feature->hstrand == 1 ) {
- $hit_start = $feature->hstart();
- } else {
- $hit_end = $feature->hend();
- }
-
- #split the feature into a seperate feature for each raw contig
- SPLIT: for( my $i=0; $i <= $#mapped; $i++ ) {
- if($mapped[$i]->isa("Bio::EnsEMBL::Mapper::Gap")){
- $self->warn("piece of evidence lies on gap\n");
- next SPLIT;
- }
-
- #caculate query and hit length for each portion of the split feature
- #may need to round hit length to avoid 'partial' peptide
-
- # decision of Michele to not cover split codons
-
- my $query_length = ($mapped[$i]->end - $mapped[$i]->start + 1);
- my $query_start = $mapped[$i]->start();
- my $query_end = $mapped[$i]->end();
-
- my $hit_length;
-
- if($self->_query_unit == $self->_hit_unit){
-
- # DnaDna and PepPep case
- $hit_length = $query_length;
-
- } elsif( $self->_query_unit > $self->_hit_unit ){
-
- # DnaPepAlign case
- # my $tmp = ($query_length/$self->_query_unit);
- # $hit_length = sprintf "%.0f", $tmp; #round value up or down
-
- $hit_length = int(( $query_length - $codon_unused_bases ) /
- $self->_query_unit() );
-
- if( $codon_unused_bases ) {
- if( $feature->hstrand() == 1 ) {
- $hit_start++;
- } else {
- $hit_end--;
- }
- }
-
- my $used_bases = $query_length - $codon_unused_bases -
- $hit_length*$self->_query_unit();
-
- if( $mapped[$i]->strand() == -1 ) {
- $query_end -= $codon_unused_bases;
- $query_start += $used_bases;
- } else {
- $query_start += $codon_unused_bases;
- $query_end -= $used_bases;
- }
-
-
- # new rest at the end ...
- if( $used_bases ) {
- $codon_unused_bases = 3 - $used_bases;
- } else {
- $codon_unused_bases = 0;
- }
-
- } elsif($self->_hit_unit > $self->_query_unit){
-
- # PepDnaAlign case (rare)
- my $tmp = ($query_length*$self->_hit_unit);
- $hit_length = sprintf "%.0f", $tmp; #round value up or down
- }
-
- if($hit_length == 0){
- next SPLIT;
- }
-
- if( $feature->hstrand() == 1 ) {
- $hit_end = ($hit_start + $hit_length) - 1;
- } else {
- $hit_start = ( $hit_end - $hit_length + 1 );
- }
-
-
- my $rawContig = $rcAdaptor->fetch_by_dbID( $mapped[$i]->id() );
-
- #create the new feature
- my $new_feature = Bio::EnsEMBL::FeaturePair->new;
- $new_feature->start($query_start);
- $new_feature->end($query_end);
- $new_feature->strand($mapped[$i]->strand);
- $new_feature->score($feature->score);
- $new_feature->percent_id($feature->percent_id);
- $new_feature->p_value($feature->p_value);
- $new_feature->hstart($hit_start);
- $new_feature->hend($hit_end);
- $new_feature->hstrand($feature->hstrand);
- $new_feature->hseqname($feature->hseqname);
- $new_feature->analysis($feature->analysis);
- $new_feature->contig($rawContig);
-
- push(@out, $new_feature);
-
- if( $feature->hstrand() == 1 ) {
- $hit_start = ($hit_end + 1);
- } else {
- $hit_end = $hit_start -1;
- }
- }
- }else{
- #feature maps to single contig
-
- if($mapped[0]->isa("Bio::EnsEMBL::Mapper::Gap")){
- $self->warn("piece of evidence lies on gap\n");
- return;
- }
-
- #create the new feature
- my $rawContig = $rcAdaptor->fetch_by_dbID( $mapped[0]->id() );
- my $new_feature = Bio::EnsEMBL::FeaturePair->new;
- $new_feature->start($mapped[0]->start);
- $new_feature->end($mapped[0]->end);
- $new_feature->strand($mapped[0]->strand);
- $new_feature->score($feature->score);
- $new_feature->percent_id($feature->percent_id);
- $new_feature->p_value($feature->p_value);
- $new_feature->hstart($feature->hstart);
- $new_feature->hend($feature->hend);
- $new_feature->hstrand($feature->hstrand);
- $new_feature->hseqname($feature->hseqname);
- $new_feature->analysis($feature->analysis);
- $new_feature->contig($rawContig);
-
- push(@out, $new_feature);
- }
-
-
- return @out;
-}
1;
diff --git a/modules/Bio/EnsEMBL/ChainedAssemblyMapper.pm b/modules/Bio/EnsEMBL/ChainedAssemblyMapper.pm
new file mode 100644
index 0000000000..cdfd072fa8
--- /dev/null
+++ b/modules/Bio/EnsEMBL/ChainedAssemblyMapper.pm
@@ -0,0 +1,548 @@
+
+#
+# Ensembl module for Bio::EnsEMBL::ChainedAssemblyMapper
+#
+# Written by Graham McVicker
+#
+# Copyright GRL and EBI
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::ChainedAssemblyMapper -
+Handles mapping between two coordinate systems using the information stored in
+the assembly table
+
+=head1 SYNOPSIS
+ $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+ $asma = $db->get_AssemblyMapperAdaptor();
+ $csa = $db->get_CoordSystemAdaptor();
+
+ my $chr_cs = $cs_adaptor->fetch_by_name('chromosome', 'NCBI33');
+ my $cln_cs = $cs_adaptor->fetch_by_name('clone');
+
+ $asm_mapper = $map_adaptor->fetch_by_CoordSystems($cs1, $cs2);
+
+ #map to contig coordinate system from chromosomal
+ @cln_coords = $asm_mapper->map('X', 1_000_000, 2_000_000, 1, $chr_cs);
+
+ #map to chromosome coordinate system from contig
+ @chr_coords = $asm_mapper->map('AL30421.1',100,10000,-1,$cln_cs);
+
+ #list contig names for a region of chromsome
+ @cln_ids = $asm_mapper->list_ids('13', 1_000_000, 1, $chr_cs);
+
+ #list chromosome names for a contig region
+ @chr_ids = $asm_mapper->list_ids('AL30421.1',1,1000,-1,$cln_cs);
+
+=head1 DESCRIPTION
+
+The ChainedAssemblyMapper is an extension of the regular AssemblyMapper that
+allows for mappings between coordinate systems that require multi-step mapping.
+For example if explicit mappings are defined between the following
+coordinate systems,
+ chromosome <-> contig
+ contig <-> clone
+the ChainedAssemblyMapper would be able to perform implicit mapping between
+the chromosome and clone coordinate systems. This should be transparent to
+the user of this module, and users should not even realise that they are using
+a chained assembly mapper as opposed to a normal assembly mapper.
+
+=head1 CONTACT
+
+Post general queries to B<ensembl-dev@ebi.ac.uk>
+
+=head1 METHODS
+
+=cut
+
+
+my $FIRST = 'first';
+my $MIDDLE = 'middle';
+my $LAST = 'last';
+
+package Bio::EnsEMBL::ChainedAssemblyMapper;
+
+use strict;
+use warnings;
+use integer; #use proper arithmetic bitshifts
+
+use Bio::EnsEMBL::Mapper;
+use Bio::EnsEMBL::Mapper::RangeRegistry;
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate);
+
+my $CHUNKFACTOR = 20; #2^20 = approx 10^6
+my $MAX_PAIR_COUNT = 6000; # max size of the pair cache in the mappers
+
+=head2 new
+
+ Arg [1] : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Arg [2] : Bio::EnsEMBL::CoordSystem $src_cs
+ Arg [3] : Bio::EnsEMBL::CoordSystem $int_cs
+ Arg [4] : Bio::EnsEMBL::CoordSystem $dst_cs
+ Example : Should use AssemblyMapperAdaptor->fetch_by_CoordSystems
+ Description: Creates a new AssemblyMapper
+ Returntype : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Exceptions : thrown if wrong number of coord_systems are provided
+ Caller : AssemblyMapperAdaptor
+
+=cut
+
+sub new {
+ my ($caller,$adaptor,@coord_systems) = @_;
+
+ my $class = ref($caller) || $caller;
+
+ my $self = {};
+ bless $self, $class;
+
+ $self->adaptor($adaptor);
+
+ if(@coord_systems != 3) {
+ throw('ChainedMapper can only map between 3 coordinate systems. ' .
+ scalar(@coord_systems) . ' were provided');
+ }
+
+ # Set the component, intermediate and assembled coordinate systems
+ $self->{'first_cs'} = $coord_systems[0];
+ $self->{'mid_cs'} = $coord_systems[1];
+ $self->{'last_cs'} = $coord_systems[2];
+
+ #maps between first and intermediate coord systems
+ $self->{'first_mid_mapper'} = Bio::EnsEMBL::Mapper->new($FIRST, $MIDDLE);
+
+ #maps between last and intermediate
+ $self->{'last_mid_mapper'} = Bio::EnsEMBL::Mapper->new($LAST, $MIDDLE);
+
+ #mapper that is actually used and is loaded by the mappings generated
+ #by the other two mappers
+ $self->{'first_last_mapper'} = Bio::EnsEMBL::Mapper->new($FIRST, $LAST);
+
+ #need registries to keep track of what regions are registered in source
+ #and destination coordinate systems
+ $self->{'first_registry'} = Bio::EnsEMBL::Mapper::RangeRegistry->new();
+ $self->{'last_registry'} = Bio::EnsEMBL::Mapper::RangeRegistry->new();
+
+ return $self;
+}
+
+
+sub flush {
+ my $self = shift;
+ $self->{'first_registry'}->flush();
+ $self->{'last_registry'}->flush();
+
+ $self->{'first_mid_mapper'}->flush();
+ $self->{'last_mid_mapper'}->flush();
+ $self->{'first_last_mapper'}->flush();
+}
+
+sub size {
+ my $self = shift;
+ return ( $self->{'first_last_mapper'}->{'pair_count'} +
+ $self->{'last_mid_mapper'}->{'pair_count'} +
+ $self->{'first_mid_mapper'}->{'pair_count'} );
+}
+
+
+
+=head2 map
+
+ Arg [1] : string $frm_seq_region
+ The name of the sequence region to transform FROM
+ Arg [2] : int $frm_start
+ The start of the region to transform FROM
+ Arg [3] : int $frm_end
+ The end of the region to transform FROM
+ Arg [4] : int $strand
+ The strand of the region to transform FROM
+ Arg [5] : Bio::EnsEMBL::CoordSystem
+ The coordinate system to transform FROM
+ Example : @coords = $asm_mapper->map('X', 1_000_000, 2_000_000,
+ 1, $chr_cs);
+ Description: Transforms coordinates from one coordinate system
+ to another.
+ Returntype : List of Bio::EnsEMBL::Mapper::Coordinate and/or
+ Bio::EnsEMBL::Mapper:Gap objects
+ Exceptions : thrown if the specified TO coordinat system is not one
+ of the coordinate systems associated with this assembly mapper
+ Caller : general
+
+=cut
+
+sub map {
+ throw('Incorrect number of arguments.') if(@_ < 6);
+
+ my ($self, $frm_seq_region, $frm_start,
+ $frm_end, $frm_strand, $frm_cs, $fastmap) = @_;
+
+ my $mapper = $self->{'first_last_mapper'};
+ my $first_cs = $self->{'first_cs'};
+ my $last_cs = $self->{'last_cs'};
+
+ my $frm;
+ my $registry;
+
+ #speed critical section:
+ #try to do simple pointer equality comparisons of the coord system objects
+ #first since this is likely to work most of the time and is much faster
+ #than a function call
+
+ if($frm_cs == $first_cs ||
+ ($frm_cs != $last_cs && $frm_cs->equals($first_cs))) {
+ $frm = $FIRST;
+ $registry = $self->{'first_registry'};
+ } elsif($frm_cs == $last_cs || $frm_cs->equals($last_cs)) {
+ $frm = $LAST;
+ $registry = $self->{'last_registry'};
+ } else {
+ throw("Coordinate system " . $frm_cs->name . " " . $frm_cs->version .
+ " is neither the first nor the last coordinate system " .
+ " of this ChainedAssemblyMapper");
+ }
+
+ #the minimum area we want to register if registration is necessary is
+ #about 1MB. Break requested ranges into chunks of 1MB and then register
+ #this larger region if we have a registry miss.
+
+ #use bitwise shift for fast and easy integer multiplication and division
+ my ($min_start, $min_end);
+
+ $min_start = (($frm_start >> $CHUNKFACTOR) << $CHUNKFACTOR);
+ $min_end = ((($frm_end >> $CHUNKFACTOR) + 1) << $CHUNKFACTOR) - 1 ;
+
+ #get a list of ranges in the requested region that have not been registered,
+ #and register them at the same
+
+ #print STDERR "frm_start=$frm_start frm_end=$frm_end" .
+ # "min_start=$min_start min_end=$min_end\n";
+
+ my $ranges =
+ $registry->check_and_register($frm_seq_region, $frm_start, $frm_end,
+ $min_start, $min_end);
+
+ if(defined($ranges)) {
+ if( $self->size() > $MAX_PAIR_COUNT ) {
+ $self->flush();
+ $ranges =
+ $registry->check_and_register($frm_seq_region, $frm_start, $frm_end,
+ $min_start, $min_end);
+ }
+ $self->adaptor->register_chained($self,$frm,$frm_seq_region,$ranges);
+ }
+
+ if($fastmap) {
+ return $mapper->fastmap($frm_seq_region, $frm_start, $frm_end,
+ $frm_strand, $frm);
+ }
+
+ return $mapper->map_coordinates($frm_seq_region, $frm_start, $frm_end,
+ $frm_strand, $frm);
+}
+
+
+sub fastmap {
+ my $self = shift;
+ $self->map(@_,1);
+}
+
+
+
+=head2 list_seq_regions
+
+ Arg [1] : string $frm_seq_region
+ The name of the sequence region of interest
+ Arg [2] : int $frm_start
+ The start of the region of interest
+ Arg [3] : int $frm_end
+ The end of the region to transform of interest
+ Arg [5] : Bio::EnsEMBL::CoordSystem $frm_cs
+ The coordinate system to obtain overlapping ids of
+ Example : foreach $id ($asm_mapper->list_ids('X',1,1000,$ctg_cs)) {...}
+ Description: Retrieves a list of overlapping seq_region internal identifiers
+ of another coordinate system. This is the same as the
+ list_ids method but uses seq_region names rather internal ids
+ Returntype : List of strings
+ Exceptions : none
+ Caller : general
+
+=cut
+
+
+sub list_seq_regions {
+ throw('Incorrect number of arguments.') if(@_ != 5);
+ my($self, $frm_seq_region, $frm_start, $frm_end, $frm_cs) = @_;
+
+ #the minimum area we want to register if registration is necessary is
+ #about 1MB. Break requested ranges into chunks of 1MB and then register
+ #this larger region if we have a registry miss.
+
+ #use bitwise shift for fast and easy integer multiplication and division
+ my $min_start;
+ my $min_end;
+
+ $min_start = (($frm_start >> $CHUNKFACTOR) << $CHUNKFACTOR);
+ $min_end = ((($frm_end >> $CHUNKFACTOR) + 1) << $CHUNKFACTOR) - 1;
+
+ if($frm_cs->equals($self->{'first_cs'})) {
+ my $registry = $self->{'first_registry'};
+ my $ranges =
+ $registry->check_and_register($frm_seq_region, $frm_start, $frm_end,
+ $min_start, $min_end);
+
+ if(defined($ranges)) {
+ $self->adaptor->register_chained($self,$FIRST,$frm_seq_region,$ranges);
+ }
+
+ return map {$_->to()->id()}
+ $self->first_last_mapper()->list_pairs($frm_seq_region, $frm_start,
+ $frm_end, $FIRST);
+
+ } elsif($frm_cs->equals($self->{'last_cs'})) {
+ my $registry = $self->{'last_registry'};
+ my $ranges =
+ $registry->check_and_register($frm_seq_region, $frm_start, $frm_end,
+ $min_start, $min_end);
+
+ if(defined($ranges)) {
+ $self->adaptor->register_chained($self,$LAST,$frm_seq_region,$ranges);
+ }
+
+ return map {$_->from()->id()}
+ $self->first_last_mapper()->list_pairs($frm_seq_region, $frm_start,
+ $frm_end, $LAST);
+ } else {
+ throw("Coordinate system " . $frm_cs->name . " " . $frm_cs->version .
+ " is neither the first nor the last coordinate system " .
+ " of this ChainedAssemblyMapper");
+ }
+}
+
+
+=head2 list_ids
+
+ Arg [1] : string $frm_seq_region
+ The name of the sequence region of interest
+ Arg [2] : int $frm_start
+ The start of the region of interest
+ Arg [3] : int $frm_end
+ The end of the region to transform of interest
+ Arg [5] : Bio::EnsEMBL::CoordSystem $frm_cs
+ The coordinate system to obtain overlapping ids of
+ Example : foreach $id ($asm_mapper->list_ids('X',1,1000,$chr_cs)) {...}
+ Description: Retrieves a list of overlapping seq_region internal identifiers
+ of another coordinate system. This is the same as the
+ list_seq_regions method but uses internal identfiers rather
+ than seq_region strings
+ Returntype : List of ints
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub list_ids {
+ throw('Incorrect number of arguments.') if(@_ != 5);
+ my($self, $frm_seq_region, $frm_start, $frm_end, $frm_cs) = @_;
+
+ #retrieve the seq_region names
+ my @seq_regs =
+ $self->list_seq_regions($frm_seq_region,$frm_start,$frm_end,$frm_cs);
+
+ #The seq_regions are from the 'to' coordinate system not the
+ #from coordinate system we used to obtain them
+ my $to_cs;
+ if($frm_cs->equals($self->first_CoordSystem())) {
+ $to_cs = $self->last_CoordSystem();
+ } else {
+ $to_cs = $self->first_CoordSystem();
+ }
+
+ #convert them to ids
+ return @{$self->adaptor()->seq_regions_to_ids($to_cs, \@seq_regs)};
+}
+
+
+
+
+
+
+sub first_last_mapper {
+ my $self = shift;
+ return $self->{'first_last_mapper'};
+}
+
+sub first_middle_mapper {
+ my $self = shift;
+ return $self->{'first_mid_mapper'};
+}
+
+sub last_middle_mapper {
+ my $self = shift;
+ return $self->{'last_mid_mapper'};
+}
+
+
+sub first_CoordSystem {
+ my $self = shift;
+ return $self->{'first_cs'};
+}
+
+
+sub middle_CoordSystem {
+ my $self = shift;
+ return $self->{'mid_cs'};
+}
+
+sub last_CoordSystem {
+ my $self = shift;
+ return $self->{'last_cs'};
+}
+
+sub first_registry {
+ my $self = shift;
+ return $self->{'first_registry'};
+}
+
+sub last_registry {
+ my $self = shift;
+ return $self->{'last_registry'};
+}
+
+
+
+
+
+#
+# Methods supplied to maintain polymorphism with AssemblyMapper
+# there is no real assembled or component in the chained mapper, since the
+# ordering is arbitrary and both ends might actually be assembled, but these
+# methods provide convenient synonyms
+#
+sub mapper {
+ my $self = shift;
+ return $self->first_last_mapper();
+}
+sub assembled_CoordSystem {
+ my $self = shift;
+ return $self->{'first_cs'};
+}
+sub component_CoordSystem {
+ my $self = shift;
+ return $self->{'last_cs'};
+}
+
+
+=head2 adaptor
+
+ Arg [1] : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor $adaptor
+ Example : none
+ Description: get/set for this objects database adaptor
+ Returntype : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub adaptor {
+ my $self = shift;
+ $self->{'adaptor'} = shift if(@_);
+ return $self->{'adaptor'};
+}
+
+
+=head2 in_assembly
+
+ Description: Deprecated. Use map() or list_ids() instead
+
+=cut
+
+sub in_assembly {
+ my ($self, $object) = @_;
+
+ deprecate('Use map() or list_ids() instead.');
+
+ my $csa = $self->db->get_CoordSystemAdaptor();
+
+ my $top_level = $csa->fetch_top_level();
+
+ my $asma = $self->adaptor->fetch_by_CoordSystems($object->coord_system(),
+ $top_level);
+
+ my @list = $asma->list_ids($object->seq_region(), $object->start(),
+ $object->end(), $object->coord_system());
+
+ return (@list > 0);
+}
+
+
+=head2 map_coordinates_to_assembly
+
+ Description: DEPRECATED use map() instead
+
+=cut
+
+sub map_coordinates_to_assembly {
+ my ($self, $contig_id, $start, $end, $strand) = @_;
+
+ deprecate('Use map() instead.');
+
+ #not sure if contig_id is seq_region_id or name...
+ return $self->map($contig_id, $start, $end, $strand,
+ $self->contig_CoordSystem());
+
+}
+
+
+=head2 fast_to_assembly
+
+ Description: DEPRECATED use map() instead
+
+=cut
+
+sub fast_to_assembly {
+ my ($self, $contig_id, $start, $end, $strand) = @_;
+
+ deprecate('Use map() instead.');
+
+ #not sure if contig_id is seq_region_id or name...
+ return $self->map($contig_id, $start, $end, $strand,
+ $self->contig_CoordSystem());
+}
+
+
+=head2 map_coordinates_to_rawcontig
+
+ Description: DEPRECATED use map() instead
+
+=cut
+
+sub map_coordinates_to_rawcontig {
+ my ($self, $chr_name, $start, $end, $strand) = @_;
+
+ deprecate('Use map() instead.');
+
+ return $self->map($chr_name, $start, $end, $strand,
+ $self->assembled_CoordSystem());
+
+}
+
+=head2 list_contig_ids
+ Description: DEPRECATED Use list_ids instead
+
+=cut
+
+sub list_contig_ids {
+ my ($self, $chr_name, $start, $end) = @_;
+
+ deprecate('Use list_ids() instead.');
+
+ return $self->list_ids($chr_name, $start, $end,
+ $self->assembled_CoordSystem());
+}
+
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/Chromosome.pm b/modules/Bio/EnsEMBL/Chromosome.pm
index 3f81d04e96..2f7649d1ac 100755
--- a/modules/Bio/EnsEMBL/Chromosome.pm
+++ b/modules/Bio/EnsEMBL/Chromosome.pm
@@ -15,78 +15,70 @@ Bio::EnsEMBL::Chromosome
=head1 DESCRIPTION
-Contains very basic information of a chromosome and access methods
-for global features of a chromosome. It does not have the sequence or
-more detailed information - check out SliceAdaptor for that (you will
-want to make a slice of the chromosome)
-
-=head1 CONTACT
+The chromosome object is deprecated. Use Bio::EnsEMBL::Slice instead.
+
+=head1 CONTACT
Post questions to the EnsEMBL developer mailing list: <ensembl-dev@ebi.ac.uk>
=cut
-# Let the code begin...
package Bio::EnsEMBL::Chromosome;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::Root;
-@ISA = qw( Bio::EnsEMBL::Root );
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+use Bio::EnsEMBL::Slice;
+
+@ISA = qw( Bio::EnsEMBL::Slice );
-## Changes to new by James Smith - no longer takes statistics
-## on creation.. these get added later.
=head2 new
- Args [...] : List of named arguments
- Example : $chr = new Chromosome(-chr_name => $name,
- -dbID => $dbID,
- -adaptor => $adaptor,
- -length => $length);
- Description: Creates a new chromosome object
- Returntype : Bio::EnsEMBL::Chromosome
- Exceptions : thrown if the adaptor or chr_name argument is not supplied
- Caller : Bio::EnsEMBL::DBSQL::ChromosomeAdaptor
+ Description: The chromosome class is deprecated. Bio::EnsEMBL::Slice class
+ should be used instead.
=cut
sub new {
- my ($class,@args) = @_;
- my $self = {};
- bless($self, $class);
-
- my ( $chr_name, $chromosome_id, $adaptor, $length ) =
- $self->_rearrange([qw(CHR_NAME DBID ADAPTOR LENGTH)], @args);
-
- $self->throw("Badly formed chromosome")
- unless defined $chr_name && defined $adaptor;
-
- $self->adaptor( $adaptor );
- $self->chr_name( $chr_name );
- $self->dbID( $chromosome_id );
- $self->length( $length );
- $self->{'stats'} ={};
+ my $caller = shift;
+
+ deprecate("The Bio::EnsEMBL::Chromosome class is deprecated." .
+ "The Bio::EnsEMBL::Slice class should be used instead");
+
+ my ($adaptor, $chr_name) = rearrange(['ADAPTOR', 'CHR_NAME'], @_);
+
+ if($chr_name) {
+ if($adaptor) {
+ my $chr = $adaptor->fetch_by_region('toplevel',$chr_name);
+ bless $chr, 'Bio::EnsEMBL::Chromosome';
+ return $chr;
+ } else {
+ return $caller->SUPER::new(@_,
+ -ADAPTOR => $adaptor,
+ -SEQ_REGION_NAME => $chr_name);
+ }
+ }
+
+ my $self = $caller->SUPER::new(@_);
+ if($self->adaptor) {
+ $self->adaptor($self->adaptor->db->get_ChromosomeAdaptor);
+ }
+
return $self;
}
-=head2 chr_name
- Arg [1] : string $chr_name
- Example : none
- Description: get/set for attribute chromosome name
- Returntype : string
- Exceptions : none
- Caller : general
+#by name actually mean seq_region_name
+sub name {
+ my $self = shift;
+ return $self->seq_region_name(@_);
+}
-=cut
-sub chr_name{
- my( $self,$value ) = @_;
- $self->{'chr_name'} = $value if defined $value;
- return $self->{'chr_name'};
-}
=head2 stats
@@ -101,7 +93,7 @@ sub chr_name{
sub stats {
my $self = shift;
- return %{$self->{'stats'}};
+ return %{$self->{'stats'} || {}};
}
=head2 stat
@@ -122,59 +114,6 @@ sub stat {
return $self->{'stats'}{$key};
}
-=head2 adaptor
-
- Arg [1] : Bio::EnsEMBL::DBSQL::ChromosomeAdaptor $adaptor
- Example : none
- Description: get/set for this objects Adaptor
- Returntype : Bio::EnsEMBL::DBSQL::ChromsomeAdaptor
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub adaptor {
- my( $self,$value ) = @_;
- $self->{'adaptor'} = $value if defined $value;
- return $self->{'adaptor'};
-}
-
-=head2 dbID
-
- Arg [1] : int $dbID
- Example : none
- Description: get/set for the database internal id
- Returntype : int
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub dbID {
- my ($self, $value) = @_;
- $self->{'_dbID'} = $value if defined $value;
- return $self->{'_dbID'};
-}
-
-
-
-=head2 length
-
- Arg [1] : int $length
- Example : none
- Description: get/set for the attribute length, the Chromosomes length in
- basepairs
- Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub length {
- my ($self, $length) = @_;
- $self->{'length'} = $length if defined $length;
- return $self->{'length'};
-}
## Deprecated calls - these should now use "stat"
sub xref_genes { return $_[0]->stat('xref_genes'); }
diff --git a/modules/Bio/EnsEMBL/Clone.pm b/modules/Bio/EnsEMBL/Clone.pm
index 047b8b86aa..9ec8902291 100644
--- a/modules/Bio/EnsEMBL/Clone.pm
+++ b/modules/Bio/EnsEMBL/Clone.pm
@@ -11,275 +11,155 @@
=head1 NAME
-Bio::EnsEMBL::Clone - Object representing one clone
-
-=head1 SYNOPSIS
-
- @contig = $db->get_all_Contigs();
-
- $clone = $db->get_Clone();
-
- @genes = $clone->get_all_Genes();
+Bio::EnsEMBL::Clone - DEPRECATED use Bio::EnsEMBL::Slice instead
=head1 DESCRIPTION
-Represents information on one Clone
+DEPRECATED - Create Slices on clone regions instead
=head1 CONTACT
Post questions to the EnsEMBL developer list: <ensembl-dev@ebi.ac.uk>
-=head1 APPENDIX
-
-The rest of the documentation details each of the object methods. Internal
-methods are usually preceded with a _
-
=cut
-
-# Let the code begin...
-
-
package Bio::EnsEMBL::Clone;
use vars qw(@ISA);
use strict;
-# Object preamble - inheriets from Bio::EnsEMBL::Root
+use Bio::EnsEMBL::Slice;
+use Bio::EnsEMBL::RawContig;
-use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate);
-@ISA = qw( Bio::EnsEMBL::Root );
+@ISA = qw( Bio::EnsEMBL::Slice );
=head2 new
- Arg [1] : Bio::EnsEMBL::DBSQL::BaseAdaptor $adaptor (optional)
- The database adaptor for this clone object
- Arg [2] : int $internal_id (optional)
- The database identifier for this clone
- Arg [3] : string $id (optional)
- The name of this clone
- Arg [4] : string $embl_id (optional)
- The EMBL accession number of this clone
- Arg [5] : int $version (optional)
- The version of this clone
- Arg [6] : int $embl_version (optional)
- The EMBL version of this clone
- Arg [7] : int htg_phase (optional)
- the htg phase of this clone
- Arg [8] : date $created
- The date this clone was first entered into the database.
- Arg [9] : date $modified
- The date this clone was last modified in the database
- Example : $clone = new Bio::EnsEMBL::Clone();
- Description: Creates a new Clone object from a set of optional parameters.
- Returntype : Bio::EnsEMBL::Clone
- Exceptions : none
- Caller : general
+ Description: DEPRECATED. Use Bio::EnsEMBL::Slice instead
=cut
sub new {
- my ($class,$adaptor,$internal_id, $id, $embl_id, $version, $embl_version,
- $htg_phase, $created, $modified) = @_;
-
-
- return bless { 'adaptor' => $adaptor,
- 'dbID' => $internal_id,
- '_clone_id' => $id,
- 'embl_id' => $embl_id,
- 'version' => $version,
- 'embl_version' => $embl_version,
- 'htg_phase' => $htg_phase,
- 'created' => $created,
- 'modified' => $modified,
- }, $class;
-
-# $self->adaptor($adaptor);
-# $self->dbID($internal_id);
-# $self->id($id);
-# $self->embl_id($embl_id);
-# $self->version($version);
-# $self->embl_version($embl_version);
-# $self->htg_phase($htg_phase);
-# $self->created($created);
-# $self->modified($modified);
-
-# return $self;
-}
-
-
-
-=head2 get_all_Genes
-
- Args : none
- Example : none
- Description: gets all Genes that have coordinates on this Clone. They
- come in RawContig coords, but not all coords need to be on this
- Clone
- Returntype : list of Bio::EnsEMBL::Gene
- Exceptions : none
- Caller : general
-
-=cut
+ my $class = shift;
-sub get_all_Genes {
- my $self=shift;
+ deprecate("Bio::EnsEMBL::Clone is a deprecated class\n" .
+ "Use Bio::EnsEMBL::Slice instead");
- return $self->adaptor->get_all_Genes( $self->dbID );
+ return $class->SUPER::new(@_);
}
+
=head2 get_all_Contigs
- Args : none
- Example : none
- Description: get RawContig objects from this Clone (either manually added
- or from the database).
- Returntype : listref of Bio::EnsEMBL::RawContig
- Exceptions : none
- Caller : general
+ Description: DEPRECATED. Use Slice::project instead
=cut
sub get_all_Contigs {
my( $self ) = @_;
-
- my( $c_list );
-
- #If the contig list is not defined then an attempt is made to retrieve it
- #from the database
- unless ($c_list = $self->{'_contig_list'}) {
- my $ra = $self->adaptor->db->get_RawContigAdaptor;
- $c_list = $ra->fetch_all_by_Clone($self);
- $self->{'_contig_list'} = $c_list;
+
+ deprecate('Use Slice::project instead');
+
+ # Assume that we actually want to project to sequence level
+ my $projection = $self->project('seqlevel');
+
+ my @out;
+ foreach my $segment (@$projection) {
+ my $contig = $segment->[2];
+ #bless slices into RawContigs for backwards compatibility
+ bless $contig, "Bio::EnsEMBL::RawContig";
+ push @out, $contig;
}
- return $c_list;
+ return \@out;
}
=head2 add_Contig
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- Example : $clone->add_Contig($contig);
- Description: Manually adds a contig to this clone object. This is generally
- for database loading purposes only. I.e. contigs are added
- to clones and then the clones, attached contigs and dna
- sequences are loaded into the database. If contigs are added
- in this fashion then get_all_Contigs will only return contigs
- manually added, not contigs which may be residing in the
- database.
- Returntype : none
- Exceptions : none
- Caller : database loading scripts
+ Description: DEPRECATED. There is currently no replacement for this method
+ Possibly one should be added?
=cut
sub add_Contig {
my ($self, $contig) = @_;
- unless(defined $self->{'_contig_list'}) {
- $self->{'_contig_list'} = [];
- }
+ ### XXX Should there be a replacement for this?
+ deprecate('There is currently no replacement for this method');
- push @{$self->{'_contig_list'}}, $contig;
+ return 0;
}
=head2 delete_by_dbID
- Args : none
- Example : none
- Description: Deletes the clone, contig, dna and features for this,
- Genes are not deleted
- Returntype : none
- Exceptions : none
- Caller : general
+ Description: DEPRECATED. There is currently no replacement for this method
+ Possibly there should be one?
=cut
sub delete_by_dbID {
- my ($self)=shift;
- return $self->adaptor->delete_by_dbID($self->dbID);
+ my ($self)=shift;
+
+ ### XXX Should there be a replacement for this?
+ deprecate('There is currently no replacement for this method');
+
+ return 0;
}
=head2 get_RawContig_by_position
- Arg 1 : int $base_pair
- Example : none
- Description: returns the RawContig that contains that clone basepair
- Returntype : Bio:EnsEMBL::RawContig
- Exceptions : base_pair > 0, returns the last Contig if base_pair is outside
- Caller : general
+ Description: DEPRECATED. Use Slice::project instead
=cut
sub get_RawContig_by_position {
+ my ($self, $pos) = @_;
- my ($self, $pos) = @_;
+ deprecate('Use Slice::project instead');
+ throw("get_rawcontig_by_position error: Position must be > 0") if($pos < 1);
- if ($pos < 1 ){
- $self->throw("get_rawcontig_by_position error: Position must be > 0");
- }
-
- my $contigs = $self->get_all_Contigs();
- my @sorted_contigs =
- sort { $b->embl_offset <=> $a->embl_offset } @$contigs;
-
- foreach my $c ( @sorted_contigs ) {
- if ($pos > $c->embl_offset) {
- return $c;
- }
+ my $projection = $self->project('seqlevel');
+ foreach my $segment (@$projection) {
+ my($start,$end,$contig) = @$segment;
+ if($start <= $pos) {
+ return bless($contig, 'Bio::EnsEMBL::RawContig');
}
-
- return (undef);
+ }
+
+ return undef;
}
=head2 htg_phase
- Arg [1] : string $htg_phase
- 0,1,2,3 representing how finished the clone is
- Example : none
- Description: get/set for attribute htg_phase
- ( high throughput genome project phase )
- Returntype : string
- Exceptions : none
- Caller : general
+ Description: DEPRECATED - use $slice->get_attribute('htg_phase') instead
=cut
sub htg_phase {
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'htg_phase'} = $value;
- }
- return $obj->{'htg_phase'};
+ my $self = shift;
+ my ($htg_phase) = $self->get_attribute('htg_phase');
+ return $htg_phase;
}
=head2 created
- Arg [1] : string $created
- Example : none
- Description: get/set for attribute created.
- Gives the unix time value of the created
- datetime field, which indicates
- the first time this clone was put in ensembl
- Returntype : string
- Exceptions : none
- Caller : general
+ Description: DEPRECATED - Created information no longer stored
=cut
@@ -296,15 +176,7 @@ sub created {
=head2 modified
- Arg [1] : string $modified
- Example : none
- Description: get/set for attribute modified
- Gives the unix time value of the modified
- datetime field, which indicates
- the last time this clone was modified in ensembl
- Returntype : string
- Exceptions : none
- Caller : general
+ Description: DEPRECATE - Modified information no longer stored
=cut
@@ -321,139 +193,84 @@ sub modified {
=head2 version
- Arg [1] : string $version
- Example : none
- Description: get/set for attribute version
- this could contain an ensembl version for the clone.
- Usually we just use the EMBL one though. EnsEMBL version
- are currently not generated or maintained for clones.
- Returntype : string
- Exceptions : none
- Caller : general
+ Description: DEPRECATED - use Slice::seq_region_name
=cut
-sub version{
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'version'} = $value;
- }
- return $obj->{'version'};
-
-}
+sub version{ embl_version(@_) }
=head2 embl_version
- Arg [1] : string $embl_version
- Example : none
- Description: get/set for attribute embl_version
- Returntype : string
- Exceptions : none
- Caller : general
+ Description: DEPRECATED - use Slice::seq_region_name
=cut
sub embl_version {
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'embl_version'} = $value;
- }
- return $obj->{'embl_version'};
+ my $self = shift;
+ my $acc_ver = $self->seq_region_name();
+
+ #strip version off end of accession
+ my $ver;
+ (undef, $ver) = split(/\./, $acc_ver);
+ return $ver;
}
=head2 embl_id
- Arg [1] : string $embl_id
- Example : none
- Description: get/set for attribute embl_id
- Returntype : string
- Exceptions : none
- Caller : general
+ description: DEPRECATED - use Slice::seq_region_name
=cut
sub embl_id {
- my ($obj,$value) = @_;
- if( defined $value) {
- $obj->{'embl_id'} = $value;
- }
- return $obj->{'embl_id'};
+ my $self = shift;
+ my $acc = $self->seq_region_name();
+ #strip off version
+ ($acc) = split(/\./, $acc);
+ return $acc;
}
+#what is actually meant by clone->name is seq_region_name not name
+sub name {
+ my $self = shift;
+ return $self->seq_region_name();
+}
+
=head2 id
- Args : string $value (optional)
- The new name of this clone
- Example : $name = $clone->id();
- Description: The name of hte clone.
- Returntype : string
- Exceptions : none
- Caller : general
+Description: DEPRECATED - use Slice::seq_region_name
=cut
-sub id {
- my ($obj,$value) = @_;
- if( defined $value) {
- $obj->{'_clone_id'} = $value;
- }
- return $obj->{'_clone_id'};
-
-}
+sub id { embl_id(@_);}
=head2 dbID
- Arg [1] : int $dbID
- Example : none
- Description: get/set for the database internal id
- Returntype : int
+ Description: Deprecated. Use SliceAdaptor::get_seq_region_id instead
+
Exceptions : none
Caller : general, set from adaptor on store
=cut
sub dbID{
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'dbID'} = $value;
- }
- return $obj->{'dbID'};
+ my $self = shift;
+ deprecate('Use Bio::EnsEMBL::Slice instead of Bio::EnsEMBL::RawContig');
+ return $self->adaptor->get_seq_region_id($self);
}
-=head2 adaptor
- Arg [1] : Bio::EnsEMBL::DBSQL::CloneAdaptor $adaptor
- Example : none
- Description: get/set for this objects Adaptor
- Returntype : Bio::EnsEMBL::DBSQL::CloneAdaptor
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub adaptor {
- my ($obj,$value) = @_;
- if( defined $value) {
- $obj->{'adaptor'} = $value;
- }
- return $obj->{'adaptor'};
-
-}
1;
diff --git a/modules/Bio/EnsEMBL/Container.pm b/modules/Bio/EnsEMBL/Container.pm
index 1938879f41..388442849e 100644
--- a/modules/Bio/EnsEMBL/Container.pm
+++ b/modules/Bio/EnsEMBL/Container.pm
@@ -29,10 +29,9 @@ use strict;
package Bio::EnsEMBL::Container;
-use vars ('@ISA', '$AUTOLOAD');
-use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
-@ISA = qw(Bio::EnsEMBL::Root);
+use vars ('$AUTOLOAD');
=head2 new
@@ -42,7 +41,7 @@ use Bio::EnsEMBL::Root;
Example : $dba_holder = new Bio::EnsEMBL::DBSQL::DBAdaptorHolder($dba);
Description: Creates a new DBAdaptor holder object that forwards calls to
$dba and breaks circular references to $dba upon destruction.
- Returntype : Bio::EnsEMBL::DBAdaptorHolder
+ Returntype : Bio::EnsEMBL::Container
Exceptions : none
Caller : Bio::EnsEMBL::DBAdaptor
@@ -51,15 +50,11 @@ use Bio::EnsEMBL::Root;
sub new {
my ($class, $object) = @_;
- my $self = $class->SUPER::new();
-
unless($object) {
- $self->throw("object argument is required");
+ throw("object argument is required");
}
- $self->_obj($object);
-
- return $self;
+ return bless {'_obj' => $object}, $class;
}
@@ -166,7 +161,7 @@ sub AUTOLOAD {
return $self->_obj->$method(@args);
} else {
# Method does not exist
- $self->throw("method '$method' does not exist in '". ref($self->_obj) ."'");
+ throw("method '$method' does not exist in '". ref($self->_obj) ."'");
}
}
@@ -190,12 +185,12 @@ sub AUTOLOAD {
sub DESTROY {
my $self = shift;
- # print STDERR "Container::DESTROY : Breaking circular references:\n";
+ #print STDERR "Container::DESTROY : Breaking circular references:\n";
my $obj = $self->_obj;
if(!$obj) {
- warn("Bio::EnsEMBL::Container: potential memory leak, contained"
+ warning("Bio::EnsEMBL::Container: potential memory leak, contained"
. " object is not defined during garbage collection.");
} elsif($obj->can('deleteObj')) {
$obj->deleteObj();
diff --git a/modules/Bio/EnsEMBL/CoordSystem.pm b/modules/Bio/EnsEMBL/CoordSystem.pm
new file mode 100644
index 0000000000..73dfa98d76
--- /dev/null
+++ b/modules/Bio/EnsEMBL/CoordSystem.pm
@@ -0,0 +1,247 @@
+#
+# EnsEMBL module for Bio::EnsEMBL::CoordSystem
+#
+
+=head1 NAME
+
+Bio::EnsEMBL::CoordSystem
+
+=head1 SYNOPSIS
+
+ my $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+
+ my $csa = $db->get_CoordSystemAdaptor();
+
+ #
+ # Get all coord systems in the database:
+ #
+ foreach my $cs (@{$csa->fetch_all()}) {
+ my $str = join ':', $cs->name(),$cs->version(),$cs->dbID();
+ print "$str\n";
+ }
+
+=head1 DESCRIPTION
+
+This is a simple object which contains a few coordinate system attributes:
+name, internal identifier, version. A coordinate system is uniquely defined
+by its name and version. A version of a coordinate system applies to all
+sequences within a coordinate system. This should not be confused with
+individual sequence versions.
+
+Take for example the Human assembly. The version 'NCBI33' applies to
+to all chromosomes in the NCBI33 assembly (that is the entire 'chromosome'
+coordinate system). The 'clone' coordinate system in the same database would
+have no version however. Although the clone sequences have their own sequence
+versions, there is no version which applies to the entire set of clones.
+
+Coordinate system objects are immutable. Their name and version, and other
+attributes may not be altered after they are created.
+
+=head1 AUTHOR - Graham McVicker
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL development list ensembl-dev@ebi.ac.uk
+
+=head1 METHODS
+
+=cut
+
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::CoordSystem;
+
+use Bio::EnsEMBL::Storable;
+
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::Storable);
+
+
+=head2 new
+
+ Arg [..] : List of named arguments:
+ -NAME - The name of the coordinate system
+ -VERSION - (optional) The version of the coordinate system
+ -TOP_LEVEL - (optional) Sets whether this is a top-level coord
+ system. Default = 0
+ -SEQUENCE_LEVEL - (optional) Sets whether this is a sequence
+ level coordinate system. Default = 0
+ -DEFAULT - (optional)
+ Whether this is the default version of the
+ coordinate systems of this name. Default = 0
+ -DBID - (optional) The internal identifier of this
+ coordinate system
+ -ADAPTOR - (optional) The adaptor which provides database
+ interaction for this object
+ Example : $cs = Bio::EnsEMBL::CoordSystem->new(-NAME => 'chromosome',
+ -VERSION => 'NCBI33',
+ -DBID => 1,
+ -ADAPTOR => adaptor,
+ -DEFAULT => 1,
+ -SEQUENCE_LEVEL => 0,
+ -TOP_LEVEL => 1);
+ Description: Creates a new CoordSystem object representing a coordinate
+ system.
+ Returntype : Bio::EnsEMBL::CoordSystem
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub new {
+ my $caller = shift;
+ my $class = ref($caller) || $caller;
+
+ my $self = $class->SUPER::new(@_);
+
+ my ($name,$version, $top_level, $sequence_level, $default) =
+ rearrange(['NAME','VERSION','TOP_LEVEL', 'SEQUENCE_LEVEL',
+ 'DEFAULT'], @_);
+
+ throw('The NAME argument is required') if(!$name);
+
+ $version = '' if(!defined($version));
+
+ $top_level ||= 0;
+ $sequence_level ||= 0;
+ $default ||= 0;
+
+ $self->{'version'} = $version;
+ $self->{'name'} = $name;
+ $self->{'top_level'} = $top_level;
+ $self->{'sequence_level'} = $sequence_level;
+ $self->{'default'} = $default;
+
+ return $self;
+}
+
+
+=head2 name
+
+ Arg [1] : (optional) string $name
+ Example : print $coord_system->name();
+ Description: Getter for the name of this coordinate system
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub name {
+ my $self = shift;
+ return $self->{'name'};
+}
+
+
+
+=head2 version
+
+ Arg [1] : none
+ Example : print $coord->version();
+ Description: Getter for the version of this coordinate system. This
+ will return an empty string if no version is defined for this
+ coordinate system.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub version {
+ my $self = shift;
+ return $self->{'version'};
+}
+
+
+
+
+=head2 equals
+
+ Arg [1] : Bio::EnsEMBL::CoordSystem $cs
+ The coord system to compare to for equality.
+ Example : if($coord_sys->equals($other_coord_sys)) { ... }
+ Description: Compares 2 coordinate systems and returns true if they are
+ equivalent. The definition of equivalent is sharing the same
+ name and version.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub equals {
+ my $self = shift;
+ my $cs = shift;
+
+ if(!$cs || !ref($cs) || !$cs->isa('Bio::EnsEMBL::CoordSystem')) {
+ throw('Argument must be a Bio::EnsEMBL::CoordSystem');
+ }
+
+ if($self->{'version'} eq $cs->version() && $self->{'name'} eq $cs->name()) {
+ return 1;
+ }
+
+ return 0;
+}
+
+
+
+
+=head2 is_top_level
+
+ Arg [1] : none
+ Example : if($coord_sys->is_top_level()) { ... }
+ Description: Returns true if this is a top level coordinate system
+ Returntype : 0 or 1
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub is_top_level {
+ my $self = shift;
+ return $self->{'top_level'};
+}
+
+
+=head2 is_sequence_level
+
+ Arg [1] : none
+ Example : if($coord_sys->is_sequence_level()) { ... }
+ Description: Returns true if this is a sequence level coordinate system
+ Returntype : 0 or 1
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub is_sequence_level {
+ my $self = shift;
+ return $self->{'sequence_level'};
+}
+
+
+=head2 is_default
+
+ Arg [1] : none
+ Example : if($coord_sys->is_default()) { ... }
+ Description: Returns true if this coordinate system is the default
+ version of the coordinate system of this name.
+ Returntype : 0 or 1
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub is_default {
+ my $self = shift;
+ return $self->{'default'};
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/DBEntry.pm b/modules/Bio/EnsEMBL/DBEntry.pm
index 3a4266f53a..308917b4a2 100644
--- a/modules/Bio/EnsEMBL/DBEntry.pm
+++ b/modules/Bio/EnsEMBL/DBEntry.pm
@@ -33,17 +33,20 @@ methods are usually preceded with a _
=cut
-# Let the code begin...
-
package Bio::EnsEMBL::DBEntry;
+use Bio::EnsEMBL::Storable;
use Bio::Annotation::DBLink;
+
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
+
use vars qw(@ISA $AUTOLOAD);
use strict;
-@ISA = qw( Bio::EnsEMBL::Root Bio::Annotation::DBLink );
+@ISA = qw( Bio::EnsEMBL::Storable Bio::Annotation::DBLink );
=head2 new_fast
@@ -93,19 +96,13 @@ sub new {
my $self = bless {},$class;
my ( $adaptor, $dbID, $primary_id, $version,
- $dbname, $release, $display_id, $description ) = $self->_rearrange
- ( [ qw { ADAPTOR
- DBID
- PRIMARY_ID
- VERSION
- DBNAME
- RELEASE
- DISPLAY_ID
- DESCRIPTION
- }], @args );
-
- if( defined $adaptor ) { $self->adaptor( $adaptor )}
- if( defined $dbID ) { $self->dbID( $dbID ) }
+ $dbname, $release, $display_id, $description ) =
+ rearrange ( ['ADAPTOR','DBID','PRIMARY_ID','VERSION',
+ 'DBNAME','RELEASE','DISPLAY_ID','DESCRIPTION'], @args );
+
+ $self->{'adaptor'} = $adaptor;
+ $self->{'dbID'} = $dbID;
+
if( defined $primary_id ) { $self->primary_id( $primary_id ) }
if( defined $version ) { $self->version( $version ) } else
{ $self->version( "" ); }
@@ -113,7 +110,7 @@ sub new {
if( defined $release) { $self->release( $release ) }
if( defined $display_id) { $self->display_id( $display_id ) }
if( defined $description) { $self->description($description) }
- $self->{_synonyms} = [];;
+ $self->{synonyms} = [];;
return $self;
}
@@ -135,9 +132,9 @@ sub new {
sub primary_id {
my ( $self, $arg ) = @_;
if( defined $arg ) {
- $self->{_primary_id} = $arg;
+ $self->{primary_id} = $arg;
}
- return $self->{_primary_id};
+ return $self->{primary_id};
}
@@ -158,9 +155,9 @@ sub primary_id {
sub display_id{
my ( $self, $arg ) = @_;
if( defined $arg ) {
- $self->{_display_id} = $arg;
+ $self->{display_id} = $arg;
}
- return $self->{_display_id};
+ return $self->{display_id};
}
@@ -180,9 +177,9 @@ sub display_id{
sub dbname {
my ( $self, $arg ) = @_;
if( defined $arg ) {
- $self->{_dbname} = $arg;
+ $self->{dbname} = $arg;
}
- return $self->{_dbname};
+ return $self->{dbname};
}
@@ -239,59 +236,12 @@ sub optional_id {
sub release {
my ( $self, $arg ) = @_;
if( defined $arg ) {
- $self->{_release} = $arg;
- }
- return $self->{_release};
-}
-
-
-
-=head2 adaptor
-
- Arg [1] : (optional) Bio::EnsEMBL::DBSQL::DBEntryAdaptor $arg
- Example : $adaptor = $dbentry->adaptor;
- Description: Getter/Setter for the adaptor used by this object for database
- interaction. This attribute is set by the adaptor when this
- object is stored in the database or retrieved from the database.
- Returntype : Bio::EnsEMBL::DBSQL::DBEntryAdaptor
- Exceptions : none
- Caller : general
-
-=cut
-
-sub adaptor {
- my ( $self, $arg ) = @_;
- if( defined $arg ) {
- $self->{_adaptor} = $arg;
+ $self->{release} = $arg;
}
- return $self->{_adaptor};
+ return $self->{release};
}
-
-=head2 dbID
-
- Arg [1] : (optional) int
- Example : $dbID = $dbentry->dbID;
- Description: Getter/Setter for this objects unique database identifier. This
- attribute is set the adaptor when this object is store in the
- database or retrieved from the database.
- Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub dbID {
- my ( $self, $arg ) = @_;
- if( defined $arg ) {
- $self->{_dbID} = $arg;
- }
- return $self->{_dbID};
-}
-
-
-
=head2 version
Arg [1] : string $version
@@ -306,9 +256,9 @@ sub dbID {
sub version {
my ( $self, $arg ) = @_;
if( defined $arg ) {
- $self->{_version} = $arg;
+ $self->{version} = $arg;
}
- return $self->{_version};
+ return $self->{version};
}
@@ -328,9 +278,9 @@ sub version {
sub description {
my ( $self, $arg ) = @_;
if( defined $arg ) {
- $self->{_description} = $arg;
+ $self->{description} = $arg;
}
- return $self->{_description};
+ return $self->{description};
}
@@ -350,7 +300,7 @@ sub description {
sub add_synonym {
my ( $self, $arg ) = @_;
if( defined $arg ) {
- push( @{$self->{_synonyms}}, $arg );
+ push( @{$self->{synonyms}}, $arg );
}
}
@@ -368,7 +318,7 @@ sub add_synonym {
sub get_all_synonyms {
my $self = shift;
- return $self->{_synonyms};
+ return $self->{synonyms};
}
@@ -385,7 +335,7 @@ sub get_all_synonyms {
sub flush_synonyms {
my $self = shift;
- $self->{_synonyms} = [];
+ $self->{synonyms} = [];
}
@@ -404,9 +354,9 @@ sub flush_synonyms {
sub status{
my ( $self, $arg ) = @_;
if( defined $arg ) {
- $self->{_status} = $arg;
+ $self->{status} = $arg;
}
- return $self->{_status};
+ return $self->{status};
}
@@ -434,19 +384,14 @@ sub comment {
=head2 get_synonyms
- Arg [1] : none
- Example : none
Description: DEPRECATED use get_all_synonyms instead
- Returntype : none
- Exceptions : none
- Caller : none
=cut
sub get_synonyms {
my $self = shift;
- $self->warn("get_synonyms has been rename get_all_synonyms\n" . caller);
+ deprecate("get_synonyms has been renamed get_all_synonyms.");
return $self->get_all_synonyms;
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/AnalysisAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/AnalysisAdaptor.pm
index 86a503a9e1..51c8ce4621 100755
--- a/modules/Bio/EnsEMBL/DBSQL/AnalysisAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/AnalysisAdaptor.pm
@@ -16,8 +16,13 @@ Bio::EnsEMBL::DBSQL::AnalysisAdaptor
=head1 SYNOPSIS
- $analysisAdaptor = $db_adaptor->getAnalysisAdaptor;
- $analysisAdaptor = $analysisobj->getAnalysisAdaptor;
+ use Bio::EnsEMBL::DBSQL::DBAdaptor;
+
+ $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+
+ $analysis_adaptor = $db_adaptor->get_AnalysisAdaptor;
+
+ my $analysis = $analysis_adaptor->fetch_by_logic_name('genscan');
=head1 DESCRIPTION
@@ -28,19 +33,14 @@ Bio::EnsEMBL::DBSQL::AnalysisAdaptor
=head1 CONTACT
- Contact Arne Stabenau on implemetation/design detail: stabenau@ebi.ac.uk
- Contact Ewan Birney on EnsEMBL in general: birney@sanger.ac.uk
-
-=head1 APPENDIX
+ Post questions/comments to the EnsEMBL development list:
+ ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-# Let the code begin...
-
-
package Bio::EnsEMBL::DBSQL::AnalysisAdaptor;
use Bio::EnsEMBL::Analysis;
@@ -52,7 +52,6 @@ use strict;
@ISA = qw( Bio::EnsEMBL::DBSQL::BaseAdaptor);
-
=head2 new
Args : Bio::EnsEMBL::DBSQL::DBAdaptor
@@ -120,13 +119,6 @@ sub fetch_all {
return \@ana;
}
-sub deleteObj {
- my( $self ) = @_;
-
- $self->{_cache} = undef;
- $self->{_logic_name_cache} = undef;
- $self->SUPER::deleteObj;
-}
=head2 fetch_by_dbID
@@ -174,6 +166,15 @@ sub fetch_by_dbID {
}
+sub deleteObj {
+ my( $self ) = @_;
+
+ $self->{_cache} = undef;
+ $self->{_logic_name_cache} = undef;
+ $self->SUPER::deleteObj;
+}
+
+
=head2 fetch_by_logic_name
Arg [1] : string $logic_name the logic name of the analysis to retrieve
@@ -218,8 +219,8 @@ sub fetch_by_logic_name {
$analysis = $self->_objFromHashref( $rowHashRef );
#place the analysis in the caches, cross referenced by dbID and logic_name
- $self->{_cache}{$analysis->dbID()} = $analysis;
- $self->{_logic_name_cache}{lc($logic_name)} = $analysis;
+ $self->{_cache}->{$analysis->dbID()} = $analysis;
+ $self->{_logic_name_cache}->{lc($logic_name)} = $analysis;
return $analysis;
}
@@ -243,28 +244,24 @@ sub store {
my $self = shift;
my $analysis = shift;
-
+
if( !defined $analysis || !ref $analysis) {
- $self->throw("called store on AnalysisAdaptor with a [$analysis]");
+ throw("called store on AnalysisAdaptor with a [$analysis]");
}
- $analysis->dbID && $analysis->adaptor && ( $analysis->adaptor() == $self ) &&
- return $analysis->dbID;
-
-
+ #if this analysis has already been stored in this db, just update the adaptor
+ #and dbID of the object and return the dbID
my $dbID;
-
if( $dbID = $self->exists( $analysis )) {
$analysis->adaptor( $self );
$analysis->dbID( $dbID );
return $dbID;
}
-
+
if( !defined $analysis->logic_name ) {
- $self->throw("Must have a logic name on the analysis object");
+ throw("Must have a logic name on the analysis object");
}
-
if($analysis->created ) {
my $sth = $self->prepare( q{
INSERT INTO analysis
@@ -346,7 +343,7 @@ sub store {
$analysis->adaptor( $self );
$analysis->dbID( $dbID );
-
+
return $dbID;
}
@@ -367,7 +364,7 @@ sub update {
my ($self, $analysis) = @_;
if (!defined $analysis || !ref $analysis) {
- $self->throw("called update on AnalysisAdaptor with a [$analysis]");
+ throw("called update on AnalysisAdaptor with a [$analysis]");
}
$analysis->dbID && ($analysis->adaptor() == $self) or
@@ -415,7 +412,7 @@ sub remove {
my $dbID;
if (!defined $analysis || !ref $analysis) {
- $self->throw("called remove on AnalysisAdaptor with a [$analysis]");
+ throw("called remove on AnalysisAdaptor with a [$analysis]");
}
unless ($dbID = $self->exists($analysis)) {
@@ -445,22 +442,16 @@ sub remove {
sub exists {
my ($self,$anal) = @_;
- unless($anal->isa("Bio::EnsEMBL::Analysis")) {
- $self->throw("Object is not a Bio::EnsEMBL::Analysis");
- }
-
-
- # objects with already have this adaptor are store here.
- if( $anal->can("adaptor") && defined $anal->adaptor &&
- $anal->adaptor == $self ) {
- if (my $id = $anal->dbID) {
- return $id;
- }
- else {
- $self->throw ("analysis does not have an analysisId");
- }
+ if(!ref($anal) || !$anal->isa("Bio::EnsEMBL::Analysis")) {
+ throw("Object is not a Bio::EnsEMBL::Analysis");
}
-
+
+ #if this analysis is stored in this db already return its dbID
+ if($anal->is_stored($self->db())) {
+ return $anal->dbID();
+ }
+
+ #this analysis object is not stored but one exactly like it may have been
foreach my $cacheId (keys %{$self->{_cache}}) {
if ($self->{_cache}->{$cacheId}->compare($anal) >= 0) {
# $anal->dbID( $cacheId );
@@ -468,6 +459,8 @@ sub exists {
return $cacheId;
}
}
+
+ #no analysis like this one exists in the database
return undef;
}
@@ -482,7 +475,7 @@ sub exists {
Caller : Bio::EnsEMBL::DBSQL::AnalsisAdaptor::fetch_* methods
=cut
-
+
sub _objFromHashref {
my $self = shift;
my $rowHash = shift;
@@ -504,30 +497,10 @@ sub _objFromHashref {
-created => $rowHash->{created},
-logic_name => $rowHash->{logic_name}
);
-
+
return $analysis;
}
-=head2 db
-
- Arg [1] : (optional) Bio::EnsEMBL::DBSQL::DBAdaptor $db
- the database used by this adaptor.
- Example : my $db = $analysis_adaptor->db()
- Description: Getter/Setter for the database this adaptor uses internally
- to fetch and store database objects.
- Returntype : Bio::EnsEMBL::DBSQL::DBAdaptor
- Exceptions : none
- Caller : BaseAdaptor::new, general
-
-=cut
-
-sub db {
- my ( $self, $arg ) = @_;
- ( defined $arg ) &&
- ($self->{_db} = $arg);
- $self->{_db};;
-}
-
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/AssemblyExceptionFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/AssemblyExceptionFeatureAdaptor.pm
new file mode 100644
index 0000000000..9654ebaded
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DBSQL/AssemblyExceptionFeatureAdaptor.pm
@@ -0,0 +1,183 @@
+#
+# EnsEMBL module for Bio::EnsEMBL::DBSQL::AssemblyExceptionFeatureAdaptor
+#
+# Cared for by Ewan Birney <birney@ebi.ac.uk>
+#
+# Copyright EMBL/EBI
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::DBSQL::AssemblyExceptionFeatureAdaptor
+
+=head1 SYNOPSIS
+
+my $assembly_exception_feature_adaptor = $database_adaptor->get_AssemblyExceptionFeatureAdaptor();
+@assembly_exception_features = $assembly_exception_feature_adaptor->fetch_by_Slice($slice);
+
+=head1 DESCRIPTION
+
+Assembly Exception Feature Adaptor - database access for assembly exception features
+
+=head1 AUTHOR - Glenn Proctor
+
+=head1 APPENDIX
+
+The rest of the documentation details each of the object methods.
+Internal methods are usually preceded with a _
+
+=cut
+
+package Bio::EnsEMBL::DBSQL::AssemblyExceptionFeatureAdaptor;
+use vars qw(@ISA);
+use strict;
+
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
+use Bio::EnsEMBL::AssemblyExceptionFeature;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
+
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
+
+=head2 generic_fetch
+
+ Arg [1] : (optional) string $constraint
+ An SQL query constraint (i.e. part of the WHERE clause)
+ Arg [2] : (optional) Bio::EnsEMBL::AssemblyMapper $mapper
+ A mapper object used to remap features
+ as they are retrieved from the database
+ Arg [3] : (optional) Bio::EnsEMBL::Slice $slice
+ A slice that features should be remapped to
+ Arg [4] : (optional) boolean $keep_all
+ Set to 1 if all features, even ones entirely off slice,
+ should be kept
+ Example : $fts = $a->generic_fetch('contig_id in (1234, 1235)', 'Swall');
+ Description: Overrides the default generic fetch for this object. Will
+ ignore slice constraints.
+ Returntype : listref of Bio::EnsEMBL::SeqFeature in contig coordinates
+ Exceptions : none
+ Caller : BaseFeatureAdaptor
+
+=cut
+
+sub generic_fetch {
+ my ($self, $constraint, $mapper, $slice, $keep_all) = @_;
+
+ my $columns = join(', ', $self->_columns());
+
+ my $db = $self->db();
+
+ my $sql = "SELECT $columns FROM assembly_exception ae";
+
+ # if there is a slice constraint we remove the constraints ...
+ unless( $constraint =~ /seq_region_id/ || $constraint eq "" ) {
+ $sql .= "WHERE $constraint";
+ }
+
+ my $sth = $db->prepare($sql);
+
+ $sth->execute;
+
+ my $res = $self->_objs_from_sth($sth, $mapper, $slice, $keep_all);
+
+ return $res;
+}
+
+=head2 _tablename
+
+ Arg [1] : none
+ Example : none
+ Description: PROTECTED implementation of superclass abstract method
+ returns the names, aliases of the tables to use for queries
+ Returntype : list of listrefs of strings
+ Exceptions : none
+ Caller : internal
+
+=cut
+
+sub _tables {
+ my $self = shift;
+
+ return ['assembly_exception', 'ae'];
+}
+
+
+=head2 _columns
+
+ Arg [1] : none
+ Example : none
+ Description: PROTECTED implementation of superclass abstract method
+ returns a list of columns to use for queries
+ Returntype : list of strings
+ Exceptions : none
+ Caller : internal
+
+=cut
+
+sub _columns {
+ my $self = shift;
+
+ return qw( ae.seq_region_id ae.seq_region_start ae.seq_region_end ae.exc_type ae.exc_seq_region_id ae.exc_seq_region_start ae.exc_seq_region_end );
+}
+
+
+=head2 _objs_from_sth
+
+ Arg [1] : hash reference $hashref
+ Example : none
+ Description: PROTECTED implementation of superclass abstract method.
+ creates AssemblyExceptionFeatures from an executed DBI statement handle.
+ Returntype : list reference to Bio::EnsEMBL::AssemblyExceptionFeature objects
+ Exceptions : none
+ Caller : internal
+
+=cut
+
+sub _objs_from_sth {
+
+ my ($self, $sth, $mapper, $dest_slice) = @_;
+
+ my @features;
+
+ my $slice_adaptor = $self->db()->get_SliceAdaptor();
+
+ my ($seq_region_id, $seq_region_start, $seq_region_end, $type,
+ $exc_seq_region_id, $exc_seq_region_start, $exc_seq_region_end);
+
+ $sth->bind_columns(\$seq_region_id, \$seq_region_start, \$seq_region_end, \$type,
+ \$exc_seq_region_id, \$exc_seq_region_start, \$exc_seq_region_end);
+
+ while ($sth->fetch()) {
+
+ my $sr_slice = $slice_adaptor->fetch_by_seq_region_id($seq_region_id);
+ my $exc_slice = $slice_adaptor->fetch_by_seq_region_id($exc_seq_region_id);
+
+ # each row creates TWO features, each of which has alternate_slice pointing to the "other" one
+
+ push @features,
+ Bio::EnsEMBL::AssemblyExceptionFeature->new('-start' => $seq_region_start,
+ '-end' => $seq_region_end,
+ '-strand' => 1,
+ '-adaptor' => $self,
+ '-slice' => $sr_slice,
+ '-alternate_slice' => $exc_slice,
+ '-type' => $type);
+
+ push @features,
+ Bio::EnsEMBL::AssemblyExceptionFeature->new('-start' => $exc_seq_region_start,
+ '-end' => $exc_seq_region_end,
+ '-strand' => 1,
+ '-adaptor' => $self,
+ '-slice' => $exc_slice,
+ '-alternate_slice' => $sr_slice,
+ '-type' => $type);
+
+ }
+
+ return \@features;
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/AssemblyMapperAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/AssemblyMapperAdaptor.pm
index dbd96ceef7..c85eab653e 100644
--- a/modules/Bio/EnsEMBL/DBSQL/AssemblyMapperAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/AssemblyMapperAdaptor.pm
@@ -2,22 +2,38 @@
#
# Ensembl module for Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
#
-# Written by Ewan Birney <birney@ebi.ac.uk>
#
-# Copyright GRL/EBI
+# Copyright EnsEMBL
#
# You may distribute this module under the same terms as perl itself
# POD documentation - main docs before the code
-=head1 NAME
+=Head1 NAME
-Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor - DESCRIPTION of Object
+Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
=head1 SYNOPSIS
- # $dba is an Bio::EnsEMBL::DBSQL::DBAdaptor
- my $ass_adptr = $dba->get_AssemblyMapperAdaptor;
+ my $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+
+ my $asma = $dba->get_AssemblyMapperAdaptor();
+ my $csa = $dba->get_CoordSystemAdaptor();
+
+ my $chr33_cs = $csa->fetch_by_name('chromosome', 'NCBI33');
+ my $chr34_cs = $csa->fetch_by_name('chromosome', 'NCBI34');
+ my $ctg_cs = $csa->fetch_by_name('contig');
+ my $clone_cs = $csa->fetch_by_name('clone');
+
+ my $chr_ctg_mapper =
+ $asma->fetch_by_CoordSystems($chr33_cs, $ctg_cs);
+
+ my $ncbi33_ncbi34_mapper =
+ $asm_adptr->fetch_by_CoordSystems($chr33,$chr34);
+
+ my $ctg_clone_mapper =
+ $asm_adptr->fetch_by_CoordSystems($ctg_cs,$clone_cs);
+
=head1 DESCRIPTION
@@ -25,36 +41,50 @@ Adaptor for handling Assembly mappers. This is a
I<Singleton> class. ie: There is only one per
database (C<DBAdaptor>).
+This is used to retrieve mappers between any two coordinate systems whose
+makeup is described by the assembly table. Currently one step (explicit) and
+two step (implicit) pairwise mapping is supported. In one-step mapping
+an explicit relationship between the coordinate systems is defined in the
+assembly table. In two-step 'chained' mapping no explicit mapping is present
+but the coordinate systems must share a common mapping to an intermediate
+coordinate system.
+
=head1 CONTACT
Post general queries to B<ensembl-dev@ebi.ac.uk>
-=head1 APPENDIX
-
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-# Let the code begin...
-
-
package Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor;
use vars qw(@ISA);
use strict;
-# Object preamble - inherits from Bio::EnsEMBL::Root
-
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
use Bio::EnsEMBL::AssemblyMapper;
+use Bio::EnsEMBL::ChainedAssemblyMapper;
+
+use Bio::EnsEMBL::Utils::Cache; #CPAN LRU cache
+use Bio::EnsEMBL::Utils::Exception qw(deprecate throw);
+
+use integer; #do proper arithmetic bitshifts
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+
+my $CHUNKFACTOR = 20; # 2^20 = approx. 10^6
+# if the mapper is bigger than that its flushed before registering new stuff:
+my $MAX_PAIR_COUNT = 1000;
+
+#number of seq regions to remember ids fo
+my $SEQ_REGION_CACHE_SIZE = 2500;
+
=head2 new
- Arg [1] : Bio::EnsEMBL::DBAdaptor $dbadaptor the adaptor for the database
- this assembly mapper is using.
+ Arg [1] : Bio::EnsEMBL::DBAdaptor $dbadaptor the adaptor for
+ the database this assembly mapper is using.
Example : my $asma = new Bio::EnsEMBL::AssemblyMapperAdaptor($dbadaptor);
Description: Creates a new AssemblyMapperAdaptor object
Returntype : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
@@ -68,176 +98,845 @@ sub new {
my $self = $class->SUPER::new($dbadaptor);
- $self->{'_type_cache'} = {};
+ $self->{'_asm_mapper_cache'} = {};
+
+ my %cache;
+ tie(%cache, 'Bio::EnsEMBL::Utils::Cache', $SEQ_REGION_CACHE_SIZE);
+ $self->{'_sr_id_cache'} = \%cache;
return $self;
}
+=head2 fetch_by_CoordSystems
-=head2 fetch_by_type
+ Arg [1] : Bio::EnsEMBL::CoordSystem $cs1
+ One of the coordinate systems to retrieve the mapper
+ between
+ Arg [2] : Bio::EnsEMBL::CoordSystem $cs2
+ The other coordinate system to map between
+ Description: Retrieves an Assembly mapper for two coordinate
+ systems whose relationship is described in the
+ assembly table.
- Arg [1] : char $type
- Description: Fetches a Bio::EnsEMBL::AssemblyMapper object from the adaptor
- for a particular assembly (golden path) type (e.g. NCBI_xx).
- The result is cached for a particular assembly type.
- Returntype : Bio::EnsEMBL::AssemblyMapper
+ The ordering of the coodinate systems is arbitrary.
+ The following two statements are equivalent:
+ $mapper = $asma->fetch_by_coord_systems($cs1,$cs2);
+ $mapper = $asma->fetch_by_coord_systems($cs2,$cs1);
+ Returntype : Bio::EnsEMBL::AssemblyMapper
Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Caller : general
=cut
-sub fetch_by_type{
- my ($self,$type) = @_;
-
- if( !defined $self->{'_type_cache'}->{$type} ) {
- $self->{'_type_cache'}->{$type} =
- Bio::EnsEMBL::AssemblyMapper->new($self,$type);
+sub fetch_by_CoordSystems {
+ my $self = shift;
+ my $cs1 = shift;
+ my $cs2 = shift;
+
+ if(!ref($cs1) || !$cs1->isa('Bio::EnsEMBL::CoordSystem')) {
+ throw("cs1 argument must be a Bio::EnsEMBL::CoordSystem.");
}
-
- return $self->{'_type_cache'}->{$type};
+ if(!ref($cs2) || !$cs2->isa('Bio::EnsEMBL::CoordSystem')) {
+ throw("cs2 argument must be a Bio::EnsEMBL::CoordSystem.");
+ }
+
+ if($cs1->equals($cs2)) {
+ throw("Cannot create mapper between same coord systems: " .
+ $cs1->name . " " . $cs1->version);
+ }
+
+ my $csa = $self->db->get_CoordSystemAdaptor();
+
+ #retrieve the shortest possible mapping path between these systems
+ my @mapping_path = @{$csa->get_mapping_path($cs1,$cs2)};
+
+ if(!@mapping_path) {
+ throw("There is no mapping defined between these coord systems:\n" .
+ $cs1->name() . " " . $cs1->version() . " and " . $cs2->name() . " " .
+ $cs2->version());
+ }
+
+ my $key = join(':', map({$_->dbID()} @mapping_path));
+
+ my $asm_mapper = $self->{'_asm_mapper_cache'}->{$key};
+
+ return $asm_mapper if($asm_mapper);
+
+ if(@mapping_path == 1) {
+ throw("Incorrect mapping path defined in meta table. " .
+ "0 step mapping encountered between:\n" .
+ $cs1->name() . " " . $cs1->version() . " and " . $cs2->name . " " .
+ $cs2->version());
+ }
+
+ if(@mapping_path == 2) {
+ #1 step regular mapping
+# $asm_mapper = Bio::EnsEMBL::AssemblyMapper->new($self, @mapping_path);
+ $asm_mapper = Bio::EnsEMBL::ChainedAssemblyMapper->new( $self, $mapping_path[0], undef, $mapping_path[1] );
+ $self->{'_asm_mapper_cache'}->{$key} = $asm_mapper;
+ return $asm_mapper;
+ }
+
+ if(@mapping_path == 3) {
+ #two step chained mapping
+ $asm_mapper = Bio::EnsEMBL::ChainedAssemblyMapper->new($self,@mapping_path);
+ #in multi-step mapping it is possible get requests with the
+ #coordinate system ordering reversed since both mappings directions
+ #cache on both orderings just in case
+ #e.g. chr <-> contig <-> clone and clone <-> contig <-> chr
+
+ $self->{'_asm_mapper_cache'}->{$key} = $asm_mapper;
+ $key = join(':', map({$_->dbID()} reverse(@mapping_path)));
+ $self->{'_asm_mapper_cache'}->{$key} = $asm_mapper;
+ return $asm_mapper;
+ }
+
+ throw("Only 1 and 2 step coordinate system mapping is currently\n" .
+ "supported. Mapping between " .
+ $cs1->name() . " " . $cs1->version() . " and " .
+ $cs2->name() . " " . $cs2->version() .
+ " requires ". (scalar(@mapping_path)-1) . " steps.");
}
-=head2 register_region
- Arg [1] : Bio::EnsEMBL::AssemblyMapper $assmapper
+=head2 register_assembled
+
+ Arg [1] : Bio::EnsEMBL::AssemblyMapper $asm_mapper
A valid AssemblyMapper object
- Arg [2] : char $type
- golden path type (e.g. NCBI_xx)
- Arg [3] : char $chr_name
- chromosome name (e.g. '2', 'X')
- Arg [4] : int $start
- chromosome start coordinate
- Arg [5] : int $end
- chromosome end coordinate
- Description: Declares a chromosomal region to the AssemblyMapper.
+ Arg [2] : string $asm_seq_region
+ The name of the seq_region to be registered
+ Arg [3] : int $asm_start
+ The start of the region to be registered
+ Arg [4] : int $asm_end
+ The end of the region to be registered
+ Description: Declares an assembled region to the AssemblyMapper.
This extracts the relevant data from the assembly
- table and stores it in a Bio::EnsEMBL::Mapper.
+ table and stores it in Mapper internal to the $asm_mapper.
It therefore must be called before any mapping is
attempted on that region. Otherwise only gaps will
- be returned!
+ be returned. Note that the AssemblyMapper automatically
+ calls this method when the need arises.
Returntype : none
- Exceptions : thrown if $assmapper arg is not a Bio::EnsEMBL::AssemblyMapper
- Caller : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Exceptions : throw if the seq_region to be registered does not exist
+ or if it associated with multiple assembled pieces (bad data
+ in assembly table)
+ Caller : Bio::EnsEMBL::AssemblyMapper
=cut
-sub register_region{
- my ($self, $assmapper, $type, $chr_name, $start, $end) = @_;
- $self->throw("$assmapper is not a Bio::EnsEMBL::AssemblyMapper")
- unless $assmapper->isa("Bio::EnsEMBL::AssemblyMapper");
-
- my $chr = $self->db->get_ChromosomeAdaptor()->fetch_by_chr_name( $chr_name );
- my $chr_id = $chr->dbID();
- my $max_assembly_contig = $self->db()->get_MetaContainer->get_max_assembly_contig();
-
- if (! defined $max_assembly_contig) {
- $max_assembly_contig = 1_000_000_000;
- }
-
- my $select = qq{
- select
- ass.contig_start,
- ass.contig_end,
- ass.contig_id,
- ass.contig_ori,
- ass.chr_start,
- ass.chr_end
- from
- assembly ass
- where
- ass.chromosome_id = $chr_id and
- ? <= ass.chr_end and
- ? >= ass.chr_start and
- ? <= ass.chr_start and
- ass.type = '$type'
+sub register_assembled {
+ my $self = shift;
+ my $asm_mapper = shift;
+ my $asm_seq_region = shift;
+ my $asm_start = shift;
+ my $asm_end = shift;
+
+ my $asm_cs_id = $asm_mapper->assembled_CoordSystem->dbID();
+ my $cmp_cs_id = $asm_mapper->component_CoordSystem->dbID();
+
+ #split up the region to be registered into fixed chunks
+ #this allows us to keep track of regions that have already been
+ #registered and also works under the assumption that if a small region
+ #is requested it is likely that other requests will be made in the
+ #vicinity (the minimum size registered the chunksize (2^chunkfactor)
+
+ my @chunk_regions;
+ #determine span of chunks
+ #bitwise shift right is fast and easy integer division
+
+
+ my($start_chunk, $end_chunk);
+
+ $start_chunk = $asm_start >> $CHUNKFACTOR;
+ $end_chunk = $asm_end >> $CHUNKFACTOR;
+
+ #find regions of continuous unregistered chunks
+ my $i;
+ my ($begin_chunk_region,$end_chunk_region);
+ for ($i = $start_chunk; $i <= $end_chunk; $i++) {
+ if($asm_mapper->have_registered_assembled($asm_seq_region, $i)) {
+ if(defined($begin_chunk_region)) {
+ #this is the end of an unregistered region.
+ my $region = [($begin_chunk_region << $CHUNKFACTOR),
+ (($end_chunk_region+1) << $CHUNKFACTOR)-1];
+ push @chunk_regions, $region;
+ $begin_chunk_region = $end_chunk_region = undef;
+ }
+ } else {
+ $begin_chunk_region = $i if(!defined($begin_chunk_region));
+ $end_chunk_region = $i+1;
+ $asm_mapper->register_assembled($asm_seq_region,$i);
+ }
+ }
+
+ #the last part may have been an unregistered region too
+ if(defined($begin_chunk_region)) {
+ my $region = [($begin_chunk_region << $CHUNKFACTOR),
+ (($end_chunk_region+1) << $CHUNKFACTOR) -1];
+ push @chunk_regions, $region;
+ }
+
+ return if(!@chunk_regions);
+
+ # keep the Mapper to a reasonable size
+ if( $asm_mapper->size() > $MAX_PAIR_COUNT ) {
+ $asm_mapper->flush();
+ #we now have to go and register the entire requested region since we
+ #just flushed everything
+
+ @chunk_regions = ( [ ( $start_chunk << $CHUNKFACTOR)
+ , (($end_chunk+1) << $CHUNKFACTOR)-1 ] );
+
+ for( my $i = $start_chunk; $i <= $end_chunk; $i++ ) {
+ $asm_mapper->register_assembled( $asm_seq_region, $i );
+ }
+ }
+
+ my $asm_seq_region_id =
+ $self->_seq_region_name_to_id($asm_seq_region,$asm_cs_id);
+
+ # Retrieve the description of how the assembled region is made from
+ # component regions for each of the continuous blocks of unregistered,
+ # chunked regions
+
+ my $q = qq{
+ SELECT
+ asm.cmp_start,
+ asm.cmp_end,
+ asm.cmp_seq_region_id,
+ sr.name,
+ asm.ori,
+ asm.asm_start,
+ asm.asm_end
+ FROM
+ assembly asm, seq_region sr
+ WHERE
+ asm.asm_seq_region_id = ? AND
+ ? <= asm.asm_end AND
+ ? >= asm.asm_start AND
+ asm.cmp_seq_region_id = sr.seq_region_id AND
+ sr.coord_system_id = ?
};
- my $sth = $self->prepare($select);
-
- $sth->execute( $start, $end, $start-$max_assembly_contig );
-
- while( my $arrayref = $sth->fetchrow_arrayref ) {
- my ($contig_start, $contig_end, $contig_id, $contig_ori,
- $chr_start, $chr_end) = @$arrayref;
- if( $assmapper->_have_registered_contig($contig_id) == 0 ) {
- $assmapper->_register_contig($contig_id);
- $assmapper->_mapper->add_map_coordinates(
- $contig_id, $contig_start,
- $contig_end, $contig_ori,
- $chr_name, $chr_start, $chr_end
- );
+ my $sth = $self->prepare($q);
+
+ foreach my $region (@chunk_regions) {
+ my($region_start, $region_end) = @$region;
+ $sth->execute($asm_seq_region_id, $region_start, $region_end, $cmp_cs_id);
+
+ my($cmp_start, $cmp_end, $cmp_seq_region_id, $cmp_seq_region, $ori,
+ $asm_start, $asm_end);
+
+ $sth->bind_columns(\$cmp_start, \$cmp_end, \$cmp_seq_region_id,
+ \$cmp_seq_region, \$ori, \$asm_start, \$asm_end);
+
+ #
+ # Load the unregistered regions of the mapper
+ #
+ while($sth->fetch()) {
+ next if($asm_mapper->have_registered_component($cmp_seq_region));
+ $asm_mapper->register_component($cmp_seq_region);
+ $asm_mapper->mapper->add_map_coordinates(
+ $asm_seq_region, $asm_start, $asm_end,
+ $ori,
+ $cmp_seq_region, $cmp_start, $cmp_end);
+ $self->{'_sr_id_cache'}->{"$cmp_seq_region:$cmp_cs_id"} =
+ $cmp_seq_region_id;
}
- }
+ }
+
+ $sth->finish();
}
-=head2 register_contig
- Arg [1] : Bio::EnsEMBL::AssemblyMapper $assmapper
- A valid AssemblyMapper object
- Arg [2] : char $type
- golden path type (e.g. NCBI_xx)
- Arg [3] : int $contig_id
- RawContig internal ID
+sub _seq_region_name_to_id {
+ my $self = shift;
+ my $sr_name = shift;
+ my $cs_id = shift;
+
+ ($sr_name && $cs_id) || throw('seq_region_name and coord_system_id args ' .
+ 'are required');
+
+ my $sr_id = $self->{'_sr_id_cache'}->{"$sr_name:$cs_id"};
+
+ return $sr_id if($sr_id);
+
+ # Get the seq_region_id via the name. This would be quicker if we just
+ # used internal ids instead but stored but then we lose the ability
+ # the transform accross databases with different internal ids
+
+ my $sth = $self->prepare("SELECT seq_region_id " .
+ "FROM seq_region " .
+ "WHERE name = ? AND coord_system_id = ?");
+
+ $sth->execute($sr_name, $cs_id);
+
+ if(!$sth->rows() == 1) {
+ throw("Ambiguous or non-existant seq_region [$sr_name] " .
+ "in coord system $cs_id");
+ }
+
+ ($sr_id) = $sth->fetchrow_array();
+ $sth->finish();
+
+ $self->{'_sr_id_cache'}->{"$sr_name:$cs_id"} = $sr_id;
+
+ return $sr_id;
+}
- Description: Declares a chromosomal region to the AssemblyMapper.
+
+=head2 register_component
+
+ Arg [1] : Bio::EnsEMBL::AssemblyMapper $asm_mapper
+ A valid AssemblyMapper object
+ Arg [2] : string $cmp_seq_region
+ The name of the seq_region to be registered
+ Description: Declares a component region to the AssemblyMapper.
This extracts the relevant data from the assembly
- table and stores it in a Bio::EnsEMBL::Mapper.
+ table and stores it in Mapper internal to the $asm_mapper.
It therefore must be called before any mapping is
- attempted on that region. Otherwise only gaps will be returned!
- Returntype : 1 if the contig is present in assembly,
- 0 if the contig is absent
+ attempted on that region. Otherwise only gaps will
+ be returned. Note that the AssemblyMapper automatically
+ calls this method when the need arises.
+ Returntype : none
+ Exceptions : throw if the seq_region to be registered does not exist
+ or if it associated with multiple assembled pieces (bad data
+ in assembly table)
+ Caller : Bio::EnsEMBL::AssemblyMapper
+
+=cut
+
+sub register_component {
+ my $self = shift;
+ my $asm_mapper = shift;
+ my $cmp_seq_region = shift;
+
+ my $cmp_cs_id = $asm_mapper->component_CoordSystem()->dbID();
+ my $asm_cs_id = $asm_mapper->assembled_CoordSystem()->dbID();
+
+ #do nothing if this region is already registered
+ return if($asm_mapper->have_registered_component($cmp_seq_region));
+
+ my $cmp_seq_region_id =
+ $self->_seq_region_name_to_id($cmp_seq_region, $cmp_cs_id);
+
+ # Determine what part of the assembled region this component region makes up
+
+ my $q = qq{
+ SELECT
+ asm.asm_start,
+ asm.asm_end,
+ asm.asm_seq_region_id,
+ sr.name
+ FROM
+ assembly asm, seq_region sr
+ WHERE
+ asm.cmp_seq_region_id = ? AND
+ asm.asm_seq_region_id = sr.seq_region_id AND
+ sr.coord_system_id = ?
+ };
+
+ my $sth = $self->prepare($q);
+ $sth->execute($cmp_seq_region_id, $asm_cs_id);
+
+ if($sth->rows() == 0) {
+ #this component is not used in the assembled part i.e. gap
+ $asm_mapper->register_component($cmp_seq_region_id);
+ return;
+ }
+
+ #we do not currently support components mapping to multiple assembled
+ if($sth->rows() != 1) {
+ throw("Multiple assembled regions for single " .
+ "component region cmp_seq_region_id=[$cmp_seq_region_id]");
+ }
+
+ my ($asm_start, $asm_end, $asm_seq_region_id, $asm_seq_region) =
+ $sth->fetchrow_array();
+
+ $self->{'_sr_id_cache'}->{"$asm_seq_region:$asm_cs_id"} = $asm_seq_region_id;
+
+ $sth->finish();
+
+ # Register the corresponding assembled region. This allows a us to
+ # register things in assembled chunks which allows us to:
+ # (1) Keep track of what assembled regions are registered
+ # (2) Use locality of reference (if they want something in same general
+ # region it will already be registered).
+
+ $self->register_assembled($asm_mapper,$asm_seq_region,$asm_start,$asm_end);
+}
+
+
+
+=head register_chained
+
+ Arg [1] : Bio::EnsEMBL::ChainedAssemblyMapper $casm_mapper
+ The chained assembly mapper to register regions on
+ Arg [2] : string $from ('first' or 'last')
+ The direction we are registering from, and the name of the
+ internal mapper.
+ Arg [3] : string $seq_region_name
+ The name of the seqregion we are registering on
+ Arg [4] : listref $ranges
+ A list of ranges to register (in [$start,$end] tuples).
+
+ Description: Registers a set of ranges on a chained assembly mapper.
+ This function is at the heart of the chained mapping process.
+ It retrieves information from the assembly table and
+ dynamically constructs the mappings between two coordinate
+ systems which are 2 mapping steps apart. It does this by using
+ two internal mappers to load up a third mapper which is
+ actually used by the ChainedAssemblyMapper to perform the
+ mapping.
+
+ This method must be called before any mapping is
+ attempted on regions of interest, otherwise only gaps will
+ be returned. Note that the ChainedAssemblyMapper automatically
+ calls this method when the need arises.
+ Returntype : none
+ Exceptions : throw if the seq_region to be registered does not exist
+ or if it associated with multiple assembled pieces (bad data
+ in assembly table)
+
+ throw if the mapping between the coordinate systems cannot
+ be performed in two steps, which means there is an internal
+ error in the data in the meta table or in the code that creates
+ the mapping paths.
+ Caller : Bio::EnsEMBL::AssemblyMapper
+
+
+=cut
+
+sub register_chained {
+ my $self = shift;
+ my $casm_mapper = shift;
+ my $from = shift;
+ my $seq_region_name = shift;
+ my $ranges = shift;
+
+ my ($start_name, $start_mid_mapper, $start_cs, $start_registry,
+ $end_name, $end_mid_mapper, $end_cs, $end_registry);
+
+ if($from eq 'first') {
+ $start_name = 'first';
+ $start_mid_mapper = $casm_mapper->first_middle_mapper();
+ $start_cs = $casm_mapper->first_CoordSystem();
+ $start_registry = $casm_mapper->first_registry();
+ $end_mid_mapper = $casm_mapper->last_middle_mapper();
+ $end_cs = $casm_mapper->last_CoordSystem();
+ $end_registry = $casm_mapper->last_registry();
+ $end_name = 'last';
+ } elsif($from eq 'last') {
+ $start_name = 'last';
+ $start_mid_mapper = $casm_mapper->last_middle_mapper();
+ $start_cs = $casm_mapper->last_CoordSystem();
+ $start_registry = $casm_mapper->last_registry();
+ $end_mid_mapper = $casm_mapper->first_middle_mapper();
+ $end_cs = $casm_mapper->first_CoordSystem();
+ $end_registry = $casm_mapper->first_registry();
+ $end_name = 'first';
+ } else {
+ throw("Invalid from argument: [$from], must be 'first' or 'last'");
+ }
+
+ my $combined_mapper = $casm_mapper->first_last_mapper();
+ my $mid_cs = $casm_mapper->middle_CoordSystem();
+ my $mid_name = 'middle';
+ my $csa = $self->db->get_CoordSystemAdaptor();
+
+ # Check for the simple case where the ChainedMapper is short
+ if( ! defined $mid_cs ) {
+ $start_mid_mapper = $combined_mapper;
+ }
+
+ #the SQL varies depending on whether we are coming from assembled or
+ #component coordinate system
+ #print STDERR "ASM SQL:";
+ my $asm2cmp_sth = $self->prepare(
+ 'SELECT
+ asm.cmp_start,
+ asm.cmp_end,
+ asm.cmp_seq_region_id,
+ sr.name,
+ asm.ori,
+ asm.asm_start,
+ asm.asm_end
+ FROM
+ assembly asm, seq_region sr
+ WHERE
+ asm.asm_seq_region_id = ? AND
+ ? <= asm.asm_end AND
+ ? >= asm.asm_start AND
+ asm.cmp_seq_region_id = sr.seq_region_id AND
+ sr.coord_system_id = ?');
+
+ #print STDERR "CMP SQL:";
+ my $cmp2asm_sth = $self->prepare(
+ 'SELECT
+ asm.asm_start,
+ asm.asm_end,
+ asm.asm_seq_region_id,
+ sr.name,
+ asm.ori,
+ asm.cmp_start,
+ asm.cmp_end
+ FROM
+ assembly asm, seq_region sr
+ WHERE
+ asm.cmp_seq_region_id = ? AND
+ ? <= asm.cmp_end AND
+ ? >= asm.cmp_start AND
+ asm.asm_seq_region_id = sr.seq_region_id AND
+ sr.coord_system_id = ?');
+
+ ##############
+ # obtain the first half of the mappings and load them into the start mapper
+ #
+
+ #ascertain which is component and which is actually assembled coord system
+ my @path;
+
+ # check for the simple case, where the ChainedMapper is short
+ if( defined $mid_cs ) {
+ @path = @{$csa->get_mapping_path($start_cs, $mid_cs)};
+ } else {
+ @path = @{$csa->get_mapping_path( $start_cs, $end_cs )};
+ }
+
+ if(@path != 2) {
+ my $path = join(',', map({$_->name .' '. $_->version} @path));
+ my $len = scalar(@path) - 1;
+ throw("Unexpected mapping path between start and intermediate " .
+ "coord systems (". $start_cs->name . " " . $start_cs->version .
+ " and " . $mid_cs->name . " " . $mid_cs->version . ")." .
+ "\nExpected path length 1, got $len. " .
+ "(path=$path)");
+ }
+
+ my $sth;
+ my ($asm_cs,$cmp_cs) = @path;
+ $sth = ($asm_cs->equals($start_cs)) ? $asm2cmp_sth : $cmp2asm_sth;
+
+ my $seq_region_id = $self->_seq_region_name_to_id($seq_region_name,
+ $start_cs->dbID());
+ my $mid_cs_id;
+
+ # check for the simple case where the ChainedMapper is short
+ if( defined $mid_cs ) {
+ $mid_cs_id = $mid_cs->dbID();
+ } else {
+ $mid_cs_id = $end_cs->dbID();
+ }
+
+ my @mid_ranges;
+ my @start_ranges;
+
+ #need to perform the query for each unregistered range
+ foreach my $range (@$ranges) {
+ my ($start, $end) = @$range;
+ $sth->execute($seq_region_id, $start, $end, $mid_cs_id);
+
+ #load the start <-> mid mapper with the results and record the mid cs
+ #ranges we just added to the mapper
+
+ my ($mid_start, $mid_end, $mid_seq_region_id, $mid_seq_region,
+ $ori, $start_start, $start_end);
+
+ $sth->bind_columns(\$mid_start, \$mid_end, \$mid_seq_region_id,
+ \$mid_seq_region, \$ori, \$start_start,
+ \$start_end);
+
+ while($sth->fetch()) {
+ if( defined $mid_cs ) {
+ $start_mid_mapper->add_map_coordinates
+ (
+ $seq_region_name,$start_start, $start_end, $ori,
+ $mid_seq_region, $mid_start, $mid_end
+ );
+ } else {
+ if( $from eq "first" ) {
+ $combined_mapper->add_map_coordinates
+ (
+ $seq_region_name,$start_start, $start_end, $ori,
+ $mid_seq_region, $mid_start, $mid_end
+ );
+ } else {
+ $combined_mapper->add_map_coordinates
+ (
+ $mid_seq_region, $mid_start, $mid_end, $ori,
+ $seq_region_name,$start_start, $start_end
+ );
+ }
+ }
+
+ #update sr_name cache
+ $self->{'_sr_id_cache'}->{"$mid_seq_region:$mid_cs_id"} =
+ $mid_seq_region_id;
+
+ push @mid_ranges,[$mid_seq_region_id,$mid_seq_region,
+ $mid_start,$mid_end];
+ push @start_ranges, [ $start_start, $start_end ];
+
+ #the region that we actually register may actually be larger or smaller
+ #than the region that we wanted to register.
+ #register the intersection of the region so we do not end up doing
+ #extra work later
+
+ if($start_start < $start || $start_end > $end) {
+ $start_registry->check_and_register($seq_region_name,$start_start,
+ $start_end);
+ }
+ }
+ }
+
+ # in the one step case, we load the mid ranges in the
+ # last_registry and we are done
+ if( ! defined $mid_cs ) {
+ for my $range ( @mid_ranges ) {
+ $end_registry->check_and_register( $range->[1], $range->[2],
+ $range->[3] );
+ }
+
+ # and thats it for the simple case ...
+ return;
+ }
+
+
+ ###########
+ # now the second half of the mapping
+ # perform another query and load the mid <-> end mapper using the mid cs
+ # ranges
+ #
+
+ #ascertain which is component and which is actually assembled coord system
+ @path = @{$csa->get_mapping_path($mid_cs, $end_cs)};
+ if(@path != 2) {
+ my $path = join(',', map({$_->name .' '. $_->version} @path));
+ my $len = scalar(@path)-1;
+ throw("Unexpected mapping path between intermediate and last" .
+ "coord systems (". $mid_cs->name . " " . $mid_cs->version .
+ " and " . $end_cs->name . " " . $end_cs->version . ")." .
+ "\nExpected path length 1, got $len. " .
+ "(path=$path)");
+ }
+
+ ($asm_cs,$cmp_cs) = @path;
+ $sth = ($asm_cs->equals($mid_cs)) ? $asm2cmp_sth : $cmp2asm_sth;
+
+ my $end_cs_id = $end_cs->dbID();
+ foreach my $mid_range (@mid_ranges) {
+ my ($mid_seq_region_id, $mid_seq_region,$start, $end) = @$mid_range;
+ $sth->execute($mid_seq_region_id, $start, $end, $end_cs_id);
+ #print STDERR "bind vals=($mid_seq_region_id, $start, $end, $mid_cs_id)\n";
+
+ #load the end <-> mid mapper with the results and record the mid cs
+ #ranges we just added to the mapper
+
+ my ($end_start, $end_end, $end_seq_region_id, $end_seq_region,
+ $ori, $mid_start, $mid_end);
+
+ $sth->bind_columns(\$end_start, \$end_end, \$end_seq_region_id,
+ \$end_seq_region, \$ori, \$mid_start,
+ \$mid_end);
+
+ while($sth->fetch()) {
+ #print STDERR "Adding to end<->mid mapper:\n" .
+ # "$end_seq_region:$end_start-$end_end<->$mid_seq_region:" .
+ # "$mid_start-$mid_end($ori)\n";
+ $end_mid_mapper->add_map_coordinates
+ (
+ $end_seq_region, $end_start, $end_end, $ori,
+ $mid_seq_region, $mid_start, $mid_end
+ );
+
+ #update sr_name cache
+ $self->{'_sr_id_cache'}->{"$end_seq_region:$end_cs_id"} =
+ $end_seq_region_id;
+
+ #register this region on the end coord system
+ $end_registry->check_and_register($end_seq_region, $end_start, $end_end);
+ }
+ }
+
+ #########
+ # Now that both halves are loaded
+ # Do stepwise mapping using both of the loaded mappers to load
+ # the final start <-> end mapper
+ #
+
+ foreach my $range (@start_ranges) {
+ my ($start, $end) = @$range;
+
+ my $sum = 0;
+
+ my @initial_coords = $start_mid_mapper->map_coordinates($seq_region_name,
+ $start,$end,1,
+ $start_name);
+
+ foreach my $icoord (@initial_coords) {
+ #skip gaps
+ if($icoord->isa('Bio::EnsEMBL::Mapper::Gap')) {
+ $sum += $icoord->length();
+ next;
+ }
+
+ #print STDERR "icoord: id=".$icoord->id." start=".$icoord->start." end=".
+ # $icoord->end."\n";
+
+ #feed the results of the first mapping into the second mapper
+ my @final_coords =
+ $end_mid_mapper->map_coordinates($icoord->id, $icoord->start,
+ $icoord->end,
+ $icoord->strand, $mid_name);
+
+ my $istrand = $icoord->strand();
+ foreach my $fcoord (@final_coords) {
+ #load up the final mapper
+ if($fcoord->isa('Bio::EnsEMBL::Mapper::Coordinate')) {
+ my $total_start = $start + $sum;
+ my $total_end = $total_start + $fcoord->length - 1;
+ my $ori = $fcoord->strand();
+
+ if($from eq 'first') { #the ordering we add coords must be consistant
+ $combined_mapper->add_map_coordinates(
+ $seq_region_name, $total_start, $total_end, $ori,
+ $fcoord->id(), $fcoord->start(), $fcoord->end());
+ } else {
+ $combined_mapper->add_map_coordinates(
+ $fcoord->id(), $fcoord->start(), $fcoord->end(),$ori,
+ $seq_region_name, $total_start, $total_end);
+ }
+
+ #print STDERR " fcoord: id=".$fcoord->id." start=".
+ # $fcoord->start." end=".$fcoord->end."\n";
+ #print STDERR "Loading combined mapper with : " ,
+ # "$seq_region_name:$total_start-$total_end, ($ori) <-> "
+ # .$fcoord->id.":".$fcoord->start."-".$fcoord->end."\n";
+ }
+ $sum += $fcoord->length();
+ }
+ }
+ }
+ #all done!
+}
+
+
+=head2 deleteObj
+
+ Arg [1] : none
+ Example : none
+ Description: Cleans up this objects references to other objects so that
+ proper garbage collection can occur
+ Returntype : none
Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor
+ Caller : Bio::EnsEMBL::DBConnection
+
+=cut
+
+sub deleteObj {
+ my $self = shift;
+
+ delete $self->{'_asm_mapper_cache'};
+ $self->SUPER::deleteObj();
+}
+
+
+
+=head2 seq_regions_to_ids
+
+ Arg [1] : Bio::EnsEMBL::CoordSystem $coord_system
+ Arg [2] : listref of strings $seq_regions
+ Example : my @ids = @{$asma->seq_regions_to_ids($coord_sys, \@seq_regs)};
+ Description: Converts a list of seq_region names to internal identifiers
+ using the internal cache that has accumulated while registering
+ regions for AssemblyMappers. If any requested regions are
+ not found in the cache an attempt is made to retrieve them
+ from the database.
+ Returntype : listref of ints
+ Exceptions : throw if a non-existant seqregion is provided
+ Caller : general
+
+=cut
+
+sub seq_regions_to_ids {
+ my $self = shift;
+ my $coord_system = shift;
+ my $seq_regions = shift;
+
+ my $cs_id = $coord_system->dbID();
+
+ my @out;
+
+ foreach my $sr (@$seq_regions) {
+ my $id = $self->{'_sr_id_cache'}->{"$sr:$cs_id"};
+ $id = $self->_seq_region_name_to_id($sr,$cs_id) if(!$id);
+ push @out, $id;
+ }
+
+ return \@out;
+}
+
+
+
+=head2 register_region
+
+ Description: DEPRECATED use register_assembled instead
+
+=cut
+
+sub register_region{
+ my ($self, $assmapper, $type, $chr_name, $start, $end) = @_;
+
+ deprecate('Use register_assembled instead');
+
+ $self->register_assembled($assmapper, $chr_name, $start, $end);
+}
+
+
+=head2 register_contig
+
+ Description: DEPRECATED use register_component instead
=cut
sub register_contig {
my ($self, $assmapper, $type, $contig_id ) = @_;
- my $sth = $self->prepare(qq{
- select
- c.name,
- a.chr_start,
- a.chr_end
- from
- assembly a,
- chromosome c
- where
- contig_id = '$contig_id' and
- type = '$type' and
- c.chromosome_id = a.chromosome_id
- });
-
- $sth->execute();
-
- my @ctg_list;
-
- while (my $row = $sth->fetchrow_arrayref) {
- push @ctg_list, $row;
- }
-
- if (@ctg_list == 0) {
- #$self->warn("Not found contig $contig_id");
- return ();
- }
-
- if (@ctg_list > 1) {
- $self->warn("Contig $contig_id is ambiguous in assembly type $type");
- return ();
- }
-
- my $chr_name = $ctg_list[0]->[0];
- my $chr_start = $ctg_list[0]->[1];
- my $chr_end = $ctg_list[0]->[2];
-
- return ($chr_name, $chr_start, $chr_end);
+ deprecate('Use register_component instead');
+
+ #not sure if the use is passing in a seq_region_name or a
+ #seq_region_id...
+ register_component($assmapper, $contig_id);
}
+=head2 fetch_by_type
+
+ Description: DEPRECATED use fetch_by_CoordSystems instead
+
+=cut
+
+sub fetch_by_type{
+ my ($self,$type) = @_;
+
+ deprecate('Use fetch_by_coord_systems instead');
+
+ #assume that what the user wanted was a mapper between the sequence coord
+ #level and the top coord level
+
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+
+ my $cs1 = $csa->fetch_top_level($type);
+ my $cs2 = $csa->fetch_sequence_level();
+
+ return $self->fetch_by_CoordSystems($cs1,$cs2);
+}
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/BaseAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/BaseAdaptor.pm
index 948451529d..d513d9d648 100755
--- a/modules/Bio/EnsEMBL/DBSQL/BaseAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/BaseAdaptor.pm
@@ -62,7 +62,7 @@ Other fetch functions go by the convention of
@object_array = @{$adaptor->fetch_all_by_XXXX($arguments_for_XXXX)};
-sometimes it returns an array ref denoted by the 'all' in the name of the
+sometimes it returns an array ref denoted by the 'all' in the name of the
method, sometimes an individual object. For example
$gene = $gene_adaptor->fetch_by_stable_id($stable_id);
@@ -85,19 +85,18 @@ Post questions to the EnsEMBL developer mailing list: <ensembl-dev@ebi.ac.uk>
=head1 APPENDIX
-The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
+The rest of the documentation details each of the object methods.
+Internal methods are usually preceded with a _
=cut
-
-# Let the code begin...
-
-
package Bio::EnsEMBL::DBSQL::BaseAdaptor;
use vars qw(@ISA);
use strict;
use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Utils::Exception qw(throw);
+
@ISA = qw(Bio::EnsEMBL::Root);
@@ -122,7 +121,7 @@ sub new {
bless $self,$class;
if( !defined $dbobj || !ref $dbobj ) {
- $self->throw("Don't have a db [$dbobj] for new adaptor");
+ throw("Don't have a db [$dbobj] for new adaptor");
}
if($dbobj->isa('Bio::EnsEMBL::Container')) {
diff --git a/modules/Bio/EnsEMBL/DBSQL/BaseAlignFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/BaseAlignFeatureAdaptor.pm
index f4084b75c8..bd05ec5c7f 100644
--- a/modules/Bio/EnsEMBL/DBSQL/BaseAlignFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/BaseAlignFeatureAdaptor.pm
@@ -1,9 +1,7 @@
#
-# BioPerl module for Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor
+# EnsEMBL module for Bio::EnsEMBL::BaseFeatureAdaptor
#
-# Cared for by Ewan Birney <birney@ebi.ac.uk>
-#
-# Copyright Ewan Birney
+# Copyright (c) 2003 EnsEMBL
#
# You may distribute this module under the same terms as perl itself
@@ -11,8 +9,8 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor - Abstract Base class for
- AlignFeatureAdaptors
+Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor - Abstract Base class for
+AlignFeatureAdaptors
=head1 SYNOPSIS
@@ -25,11 +23,9 @@ This is a base adaptor for align adaptors. Since DnaAlignFeatureAdaptor and
PepAlignFeatureAdaptor had almost the same functionality it made sense to
streamline by creating this superclass.
-=head1 AUTHOR - Ewan Birney
-
-Email birney@ebi.ac.uk
+=head1 CONTACT
-Describe contact details here
+Post questions/comments to the ensembl developer list: ensembl-dev@ebi.ac.uk
=head1 APPENDIX
@@ -38,46 +34,39 @@ methods are usually preceded with a _
=cut
-
-# Let the code begin...
-
-
package Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor;
use vars qw(@ISA);
use strict;
-#Object preamble - inherits from Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor
-
use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
+=head2 fetch_all_by_Slice_and_pid
-=head2 fetch_all_by_RawContig_and_pid
-
- Arg [1] : Bio::EnsEMBL::RawContig
- the contig to obtain align features from
- Arg [2] : float $pid
- a lower bound for the percentage identifier of feats to obtain
+ Arg [1] : Bio::EnsEMBL::Slice $slice
+ The slice from which to obtain align features.
+ Arg [2] : (optional) float $pid
+ a lower bound for the percentage identity of feats to obtain
Arg [3] : (optional) string $logic_name
the logic name of the type of features to obtain
- Example : $fts = $adaptor->fetch_all_by_RawContig_and_pid($contig, 50.0);
+ Example : @feats = $adaptor->fetch_all_by_Slice_and_pid($slice, 50.0);
Description: Returns a listref of features created from the database which
- are on the contig defined by $contig and with a percentage id
+ are on the Slice $slice and with a percentage identity
greater than $pid. If logic name is defined, only features
- with an analysis of type $logic_name will be returned.
- Returntype : listref of Bio::EnsEMBL::BaseAlignFeature in contig coordinates
- Exceptions : thrown if $pid is not defined
+ with an analysis of type $logic_name will be returned.
+ Returntype : listref of Bio::EnsEMBL::BaseAlignFeatures in Slice coordinates
+ Exceptions : thrown if pid is not defined
Caller : general
=cut
-sub fetch_all_by_RawContig_and_pid {
- my($self, $contig, $pid, $logic_name) = @_;
-
+sub fetch_all_by_Slice_and_pid {
+ my ($self,$slice,$pid, $logic_name) = @_;
my $constraint;
+
#get the primary table alias
my @tabs = $self->_tables;
my $alias = $tabs[0]->[1];
@@ -86,37 +75,25 @@ sub fetch_all_by_RawContig_and_pid {
$constraint = "${alias}.perc_ident > $pid";
}
- return $self->fetch_all_by_RawContig_constraint($contig,
- $constraint,
- $logic_name);
-}
-
+ if(defined $pid){
+ $constraint = "perc_ident > $pid";
+ }
+ return $self->fetch_all_by_Slice_constraint($slice, $constraint,
+ $logic_name);
+}
-=head2 fetch_all_by_Slice_and_pid
+=head2 fetch_all_by_RawContig_and_pid
- Arg [1] : Bio::EnsEMBL::Slice $slice
- The slice from which to obtain align features.
- Arg [2] : (optional) float $pid
- a lower bound for the percentage identifier of feats to obtain
- Arg [3] : (optional) string $logic_name
- the logic name of the type of features to obtain
- Example : @feats = $adaptor->fetch_all_by_Slice_and_pid($slice, 50.0);
- Description: Returns a listref of features created from the database which
- are on the Slice $slice and with a percentage id
- greater than $pid. If logic name is defined, only features
- with an analysis of type $logic_name will be returned.
- Returntype : listref of Bio::EnsEMBL::BaseAlignFeatures in Slice coordinates
- Exceptions : thrown if pid is not defined
- Caller : general
+ Description: DEPRECATED use fetch_all_by_Slice_and_pid instead
=cut
-sub fetch_all_by_Slice_and_pid {
- my ($self,$slice,$pid, $logic_name) = @_;
- my $constraint;
+sub fetch_all_by_RawContig_and_pid {
+ my($self, $contig, $pid, $logic_name) = @_;
+ my $constraint;
#get the primary table alias
my @tabs = $self->_tables;
@@ -126,13 +103,11 @@ sub fetch_all_by_Slice_and_pid {
$constraint = "${alias}.perc_ident > $pid";
}
- if(defined $pid){
- $constraint = "perc_ident > $pid";
- }
+ return $self->fetch_all_by_RawContig_constraint($contig,
+ $constraint,
+ $logic_name);
+}
- return $self->fetch_all_by_Slice_constraint($slice, $constraint,
- $logic_name);
-}
diff --git a/modules/Bio/EnsEMBL/DBSQL/BaseFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/BaseFeatureAdaptor.pm
index 352f939baa..b8a49f631f 100644
--- a/modules/Bio/EnsEMBL/DBSQL/BaseFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/BaseFeatureAdaptor.pm
@@ -1,17 +1,13 @@
#
# EnsEMBL module for Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor
#
-# Cared for by Ewan Birney <birney@ebi.ac.uk>
-#
-# Copyright Ewan Birney
+# Copyright (c) 2003 EnsEMBL
#
# You may distribute this module under the same terms as perl itself
-# POD documentation - main docs before the code
-
=head1 NAME
-Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor - Abstract Base class for
+Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor - An Abstract Base class for all
FeatureAdaptors
=head1 SYNOPSIS
@@ -22,26 +18,31 @@ abstract methods must be performed by subclasses.
=head1 DESCRIPTION
This is a base adaptor for feature adaptors. This base class is simply a way
-of eliminating code duplication through the implementation of methods
+of eliminating code duplication through the implementation of methods
common to all feature adaptors.
=head1 CONTACT
Contact EnsEMBL development list for info: <ensembl-dev@ebi.ac.uk>
+
+=head1 METHODS
+
=cut
package Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
-use vars qw(@ISA $SLICE_FEATURE_CACHE_SIZE);
+use vars qw(@ISA);
use strict;
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
use Bio::EnsEMBL::Utils::Cache;
+use Bio::EnsEMBL::Utils::Exception qw(warning throw deprecate);
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
-$SLICE_FEATURE_CACHE_SIZE = 4;
-
+my $SLICE_FEATURE_CACHE_SIZE = 4;
+my $MAX_SPLIT_QUERY_SEQ_REGIONS = 3;
=head2 new
@@ -56,26 +57,33 @@ $SLICE_FEATURE_CACHE_SIZE = 4;
=cut
sub new {
- my ($class, @args) = @_;
+ my $caller = shift;
- my $self = $class->SUPER::new(@args);
+ my $class = ref($caller) || $caller;
- #initialize caching data structures
- $self->{'_slice_feature_cache'} = {};
+ my $self = $class->SUPER::new(@_);
- tie(%{$self->{'_slice_feature_cache'}},
- 'Bio::EnsEMBL::Utils::Cache',
- $SLICE_FEATURE_CACHE_SIZE);
+ #initialize an LRU cache
+ my %cache;
+ tie(%cache, 'Bio::EnsEMBL::Utils::Cache', $SLICE_FEATURE_CACHE_SIZE);
+ $self->{'_slice_feature_cache'} = \%cache;
return $self;
}
+
=head2 generic_fetch
Arg [1] : (optional) string $constraint
An SQL query constraint (i.e. part of the WHERE clause)
- Arg [2] : (optional) string $logic_name
- the logic_name of the analysis of the features to obtain
+ Arg [2] : (optional) Bio::EnsEMBL::AssemblyMapper $mapper
+ A mapper object used to remap features
+ as they are retrieved from the database
+ Arg [3] : (optional) Bio::EnsEMBL::Slice $slice
+ A slice that features should be remapped to
+ Arg [4] : (optional) boolean $keep_all
+ Set to 1 if all features, even ones entirely off slice,
+ should be kept
Example : $fts = $a->generic_fetch('contig_id in (1234, 1235)', 'Swall');
Description: Performs a database fetch and returns feature objects in
contig coordinates.
@@ -84,56 +92,37 @@ sub new {
Caller : BaseFeatureAdaptor, ProxyDnaAlignFeatureAdaptor::generic_fetch
=cut
-
+
sub generic_fetch {
- my ($self, $constraint, $logic_name, $mapper, $slice) = @_;
-
+ my ($self, $constraint, $mapper, $slice, $keep_all) = @_;
+
my @tabs = $self->_tables;
my $columns = join(', ', $self->_columns());
-
- if($logic_name) {
- #determine the analysis id via the logic_name
- my $analysis =
- $self->db->get_AnalysisAdaptor()->fetch_by_logic_name($logic_name);
- unless(defined $analysis && $analysis->dbID() ) {
- $self->warn("No analysis for logic name $logic_name exists\n");
- return [];
- }
-
- my $analysis_id = $analysis->dbID();
-
- #get the synonym for the primary table
- my $syn = $tabs[0]->[1];
- if($constraint) {
- $constraint .= " AND ${syn}.analysis_id = $analysis_id";
- } else {
- $constraint = " ${syn}.analysis_id = $analysis_id";
- }
- }
+ my $db = $self->db();
#
# Construct a left join statement if one was defined, and remove the
# left-joined table from the table list
#
- my ($tablename, $condition) = $self->_left_join;
+ my @left_join_list = $self->_left_join();
my $left_join = '';
my @tables;
- if($tablename && $condition) {
+ if(@left_join_list) {
+ my %left_join_hash = map { $_->[0] => $_->[1] } @left_join_list;
while(my $t = shift @tabs) {
- if($tablename eq $t->[0]) {
- my $syn = $t->[1];
- $left_join = "LEFT JOIN $tablename $syn $condition";
- push @tables, @tabs;
- last;
+ if( exists $left_join_hash{ $t->[0] } ) {
+ my $condition = $left_join_hash{ $t->[0] };
+ my $syn = $t->[1];
+ $left_join .= "LEFT JOIN ".$t->[0]." $syn ON $condition ";
} else {
- push @tables, $t;
+ push @tables, $t;
}
}
} else {
@tables = @tabs;
}
-
+
#construct a nice table string like 'table1 t1, table2 t2'
my $tablenames = join(', ', map({ join(' ', @$_) } @tables));
@@ -143,7 +132,7 @@ sub generic_fetch {
my $final_clause = $self->_final_clause;
#append a where clause if it was defined
- if($constraint) {
+ if($constraint) {
$sql .= " where $constraint ";
if($default_where) {
$sql .= " and $default_where ";
@@ -155,150 +144,120 @@ sub generic_fetch {
#append additional clauses which may have been defined
$sql .= " $final_clause";
- my $sth = $self->prepare($sql);
-
- $sth->execute;
+ ###print STDERR "\n\n$sql\n\n";
+
+ my $sth = $db->prepare($sql);
+
+ $sth->execute;
+
+ my $res = $self->_objs_from_sth($sth, $mapper, $slice, $keep_all);
- return $self->_objs_from_sth($sth, $mapper, $slice);
+ return $res;
}
=head2 fetch_by_dbID
Arg [1] : int $id
- the unique database identifier for the feature to be obtained
- Example : $feat = $adaptor->fetch_by_dbID(1234);
+ The unique database identifier for the feature to be obtained
+ Example : $feat = $adaptor->fetch_by_dbID(1234));
+ $feat = $feat->transform('contig');
Description: Returns the feature created from the database defined by the
- the id $id.
- Returntype : Bio::EnsEMBL::SeqFeature
- Exceptions : thrown if $id is not defined
+ the id $id. The feature will be returned in its native
+ coordinate system. That is, the coordinate system in which it
+ is stored in the database. In order to convert it to a
+ particular coordinate system use the transfer() or transform()
+ method. If the feature is not found in the database then
+ undef is returned instead
+ Returntype : Bio::EnsEMBL::Feature or undef
+ Exceptions : thrown if $id arg is not provided
+ does not exist
Caller : general
=cut
sub fetch_by_dbID{
my ($self,$id) = @_;
-
- unless(defined $id) {
- $self->throw("fetch_by_dbID must have an id");
- }
- my @tabs = $self->_tables;
+ throw("id argument is required") if(!defined $id);
+ #construct a constraint like 't1.table1_id = 123'
+ my @tabs = $self->_tables;
my ($name, $syn) = @{$tabs[0]};
-
- #construct a constraint like 't1.table1_id = 1'
my $constraint = "${syn}.${name}_id = $id";
- #return first element of _generic_fetch list
- my ($feat) = @{$self->generic_fetch($constraint)};
+ #Should only be one
+ my ($feat) = @{$self->generic_fetch($constraint)};
+
+ return undef if(!$feat);
+
return $feat;
}
-=head2 fetch_all_by_RawContig_constraint
-
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- The contig object from which features are to be obtained
- Arg [2] : (optional) string $constraint
- An SQL query constraint (i.e. part of the WHERE clause)
- Arg [3] : (optional) string $logic_name
- the logic name of the type of features to obtain
- Example : $fs = $a->fetch_all_by_Contig_constraint($ctg,'perc_ident>5.0');
- Description: Returns a listref of features created from the database which
- are on the contig defined by $cid and fulfill the SQL constraint
- defined by $constraint. If logic name is defined, only features
- with an analysis of type $logic_name will be returned.
- Returntype : listref of Bio::EnsEMBL::SeqFeature in contig coordinates
- Exceptions : thrown if $cid is not defined
+=head2 fetch_all_by_dbID_list
+
+ Arg [1] : listref of ints $id_list
+ The unique database identifiers for the features to be obtained
+ Example : @feats = @{$adaptor->fetch_by_dbID_list([1234, 2131, 982]))};
+ Description: Returns the features created from the database defined by the
+ the ids in contained in the id list $id_list. The features
+ will be returned in their native coordinate system. That is,
+ the coordinate system in which they are stored in the database.
+ In order to convert the features to a particular coordinate
+ system use the transfer() or transform() method. If none of the
+ features are found in the database a reference to an empty
+ list is returned.
+ Returntype : listref of Bio::EnsEMBL::Features
+ Exceptions : thrown if $id arg is not provided
+ does not exist
Caller : general
=cut
-sub fetch_all_by_RawContig_constraint {
- my ($self, $contig, $constraint, $logic_name) = @_;
-
- unless( defined $contig ) {
- $self->throw("fetch_by_Contig_constraint must have an contig");
- }
+sub fetch_all_by_dbID_list {
+ my ($self,$id_list) = @_;
- unless( ref $contig && $contig->isa('Bio::EnsEMBL::RawContig')) {
- $self->throw("contig argument is not a Bio::EnsEMBL::RawContig object\n");
+ if(!defined($id_list) || ref($id_list) ne 'ARRAY') {
+ throw("id_list list reference argument is required") if(!defined $id_list);
}
- my $cid = $contig->dbID();
+ return [] if(!@$id_list);
- #get the synonym of the primary_table
+ my @out;
+ #construct a constraint like 't1.table1_id = 123'
my @tabs = $self->_tables;
- my $syn = $tabs[0]->[1];
+ my ($name, $syn) = @{$tabs[0]};
- if($constraint) {
- $constraint .= " AND ${syn}.contig_id = $cid";
- } else {
- $constraint = "${syn}.contig_id = $cid";
- }
+ #mysql is faster and we ensure that we do not exceed the max query size by
+ #splitting large queries into smaller queries of 200 ids
+ my $max_size = 200;
- return $self->generic_fetch($constraint, $logic_name);
-}
+ while(@$id_list) {
+ my @ids;
+ if(@$id_list > $max_size) {
+ @ids = splice(@$id_list, 0, $max_size);
+ } else {
+ @ids = splice(@$id_list, 0);
+ }
+
+ my $id_str;
+ if(@ids > 1) {
+ $id_str = " IN (" . join(',', @ids). ")";
+ } else {
+ $id_str = " = " . $ids[0];
+ }
-=head2 fetch_all_by_RawContig
+ my $constraint = "${syn}.${name}_id $id_str";
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- the contig from which features should be obtained
- Arg [2] : (optional) string $logic_name
- the logic name of the type of features to obtain
- Example : @fts = $a->fetch_all_by_RawContig($contig, 'wall');
- Description: Returns a list of features created from the database which are
- are on the contig defined by $cid If logic name is defined,
- only features with an analysis of type $logic_name will be
- returned.
- Returntype : listref of Bio::EnsEMBL::*Feature in contig coordinates
- Exceptions : none
- Caller : general
-
-=cut
-
-sub fetch_all_by_RawContig {
- my ( $self, $contig, $logic_name ) = @_;
+ push @out, @{$self->generic_fetch($constraint)};
+ }
- return $self->fetch_all_by_RawContig_constraint($contig, '',$logic_name);
+ return \@out;
}
-=head2 fetch_all_by_RawContig_and_score
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- the contig from which features should be obtained
- Arg [2] : (optional) float $score
- the lower bound of the score of the features to obtain
- Arg [3] : (optional) string $logic_name
- the logic name of the type of features to obtain
- Example : @fts = $a->fetch_by_RawContig_and_score(1, 50.0, 'Swall');
- Description: Returns a list of features created from the database which are
- are on the contig defined by $cid and which have score greater
- than score. If logic name is defined, only features with an
- analysis of type $logic_name will be returned.
- Returntype : listref of Bio::EnsEMBL::*Feature in contig coordinates
- Exceptions : thrown if $score is not defined
- Caller : general
-
-=cut
-
-sub fetch_all_by_RawContig_and_score{
- my($self, $contig, $score, $logic_name) = @_;
-
- my $constraint;
-
- if(defined $score){
- #get the synonym of the primary_table
- my @tabs = $self->_tables;
- my $syn = $tabs[0]->[1];
- $constraint = "${syn}.score > $score";
- }
-
- return $self->fetch_all_by_RawContig_constraint($contig, $constraint,
- $logic_name);
-}
=head2 fetch_all_by_Slice
@@ -320,7 +279,7 @@ sub fetch_all_by_RawContig_and_score{
sub fetch_all_by_Slice {
my ($self, $slice, $logic_name) = @_;
-
+
#fetch by constraint with empty constraint
return $self->fetch_all_by_Slice_constraint($slice, '', $logic_name);
}
@@ -359,7 +318,7 @@ sub fetch_all_by_Slice_and_score {
return $self->fetch_all_by_Slice_constraint($slice, $constraint,
$logic_name);
-}
+}
=head2 fetch_all_by_Slice_constraint
@@ -383,91 +342,395 @@ sub fetch_all_by_Slice_and_score {
=cut
sub fetch_all_by_Slice_constraint {
- my($self, $slice, $constraint, $logic_name) = @_;
+ my($self, $orig_slice, $original_constraint, $logic_name) = @_;
+ my @result_features;
- unless(defined $slice && ref $slice && $slice->isa("Bio::EnsEMBL::Slice")) {
- $self->throw("Slice arg must be a Bio::EnsEMBL::Slice not a [$slice]\n");
+ if(!ref($orig_slice) || !$orig_slice->isa("Bio::EnsEMBL::Slice")) {
+ throw("Slice arg must be a Bio::EnsEMBL::Slice not a [$orig_slice]\n");
}
- $logic_name = '' unless $logic_name;
- $constraint = '' unless $constraint;
+ $original_constraint ||= '';
+ $original_constraint = $self->_logic_name_to_constraint($original_constraint,
+ $logic_name);
+
+ #if the logic name was invalid, undef was returned
+ return [] if(!defined($original_constraint));
#check the cache and return if we have already done this query
- my $key = uc(join($slice->name, $constraint, $logic_name));
- return $self->{'_slice_feature_cache'}{$key}
- if $self->{'_slice_feature_cache'}{$key};
+ my $key = uc(join(':', $orig_slice->name, $original_constraint));
+
+ if(exists($self->{'_slice_feature_cache'}->{$key})) {
+ return $self->{'_slice_feature_cache'}->{$key};
+ }
+
+ my $slice_adaptor = $orig_slice->adaptor();
+
+ #retrieve normalized 'non-symlinked' slices
+ #this allows us to support haplotypes and PARs
+ my @projection =
+ @{$slice_adaptor->fetch_normalized_slice_projection($orig_slice)};
+
+ if(@projection == 0) {
+ throw('Could not retrieve normalized Slices. Database contains ' .
+ 'incorrect assembly_exception information.');
+ }
+
+ #we want to retrieve all features calculated on the FULL original slice
+ #as well as any symlinked slices.
+
+ #Filter out any partial slices from the normalized projection that are on
+ #the same seq region as the original slice
+ my @new_projection =
+ grep { $_->[2]->seq_region_name() ne $orig_slice->seq_region_name() }
+ @projection;
+
+ push( @new_projection, [ 1, $orig_slice->length(), $orig_slice ] );
+
+ #fetch features for the primary slice AND all symlinked slices
+ foreach my $segment (@new_projection) {
+ my ($offset, $slice);
+ ($offset, undef, $slice) = @$segment;
+
+ my $slice_start = $slice->start();
+ my $slice_end = $slice->end();
+ my $slice_strand = $slice->strand();
+ my $slice_cs = $slice->coord_system();
+ my $slice_seq_region = $slice->seq_region_name();
+
+ #get the synonym and name of the primary_table
+ my @tabs = $self->_tables;
+ my ($tab_name, $tab_syn) = @{$tabs[0]};
+
+ #find out what coordinate systems the features are in
+ my $csa = $self->db->get_CoordSystemAdaptor();
+ my @feat_css = @{$csa->fetch_all_by_feature_table($tab_name)};
+
+ my $asma = $self->db->get_AssemblyMapperAdaptor();
+ my @features;
+
+ # fetch the features from each coordinate system they are stored in
+ COORD_SYSTEM: foreach my $feat_cs (@feat_css) {
+ my $mapper;
+ my @coords;
+ my @ids;
+
+ if($feat_cs->equals($slice_cs)) {
+ #no mapping is required if this is the same coord system
+ my $constraint = $original_constraint;
+
+ # obtain seq_region_id of this slice from db
+ my $seq_region_id =
+ $self->db->get_SliceAdaptor->get_seq_region_id($slice);
+ $constraint .= " AND " if($constraint);
+ $constraint .=
+ "${tab_syn}.seq_region_id = $seq_region_id AND " .
+ "${tab_syn}.seq_region_start <= $slice_end AND " .
+ "${tab_syn}.seq_region_end >= $slice_start";
+ my $fs = $self->generic_fetch($constraint,undef,$slice);
+
+ #features may still have to have coordinates made relative to slice
+ #start
+ $fs = $self->_remap($fs, $mapper, $slice);
+
+ push @features, @$fs;
+ } else {
+ $mapper = $asma->fetch_by_CoordSystems($slice_cs, $feat_cs);
+
+ # Get a list of coordinates and corresponding internal ids for the
+ # regions we are interested in
+ @coords = $mapper->map($slice_seq_region, $slice_start, $slice_end,
+ $slice_strand, $slice_cs);
+
+ @coords = grep {!$_->isa('Bio::EnsEMBL::Mapper::Gap')} @coords;
+
+ next COORD_SYSTEM if(!@coords);
+
+ @ids = map {$_->id()} @coords;
+ @ids = @{$asma->seq_regions_to_ids($feat_cs, \@ids)};
+
+ #if the regions are large and only partially spanned
+ #it is faster to to limit the query with start and end constraints
+ #however, it is difficult to tell if a region is large and only
+ #partially wanted. The easy approach is just to limit the queries if
+ #there are less than a certain number of regions. As well seperate
+ #queries are needed otherwise the indices will not be useful
+ if(@coords > $MAX_SPLIT_QUERY_SEQ_REGIONS) {
+ #do one query, and do not limit with start / end constraints
+ my $constraint = $original_constraint;
+ my $id_str = join(',', @ids);
+ $constraint .= " AND " if($constraint);
+ $constraint .= "${tab_syn}.seq_region_id IN ($id_str)";
+
+ my $fs =
+ $self->generic_fetch($constraint, $mapper, $slice);
+
+ $fs = $self->_remap($fs, $mapper, $slice);
+
+ push @features, @$fs;
+
+ } else {
+ #do multiple split queries using start / end constraints
+ my $len = @coords;
+ for(my $i = 0; $i < $len; $i++) {
+ my $constraint = $original_constraint;
+ $constraint .= " AND " if($constraint);
+ $constraint .=
+ "${tab_syn}.seq_region_id = " . $ids[$i] . " AND " .
+ "${tab_syn}.seq_region_start <= " . $coords[$i]->end() . " AND ".
+ "${tab_syn}.seq_region_end >= " . $coords[$i]->start();
+ my $fs = $self->generic_fetch($constraint,$mapper,$slice);
+
+ $fs = $self->_remap($fs, $mapper, $slice);
+
+ push @features, @$fs;
+ }
+ }
+ }
+ } #COORD system loop
- my $slice_start = $slice->chr_start();
- my $slice_end = $slice->chr_end();
- my $slice_strand = $slice->strand();
-
- my $mapper =
- $self->db->get_AssemblyMapperAdaptor->fetch_by_type($slice->assembly_type);
+ #if this was a symlinked slice offset the feature coordinates as needed
+ if($slice != $orig_slice) {
+ foreach my $f (@features) {
+ #function calls are slow!
+ if($offset != 1) {
+ $f->{'start'} += $offset-1;
+ $f->{'end'} += $offset-1;
+ }
+ $f->{'slice'} = $orig_slice;
+ push @result_features, $f;
+ }
+ } else {
+ push @result_features, @features;
+ }
+
+ } #slice & symmlinked slice loop
- #get the list of contigs this slice is on
- my @cids =
- $mapper->list_contig_ids( $slice->chr_name, $slice_start ,$slice_end );
-
- return [] unless scalar(@cids);
+ $self->{'_slice_feature_cache'}->{$key} = \@result_features;
- my $cid_list = join(',',@cids);
+ return \@result_features;
+}
- #get the synonym of the primary_table
- my @tabs = $self->_tables;
- my $syn = $tabs[0]->[1];
- #construct the SQL constraint for the contig ids
- if($constraint) {
- $constraint .= " AND ${syn}.contig_id IN ($cid_list)";
- } else {
- $constraint = "${syn}.contig_id IN ($cid_list)";
+#
+# Helper function containing some common feature storing functionality
+#
+# Given a Feature this will return a copy (or the same feature if no changes
+# to the feature are needed) of the feature which is relative to the start
+# of the seq_region it is on. The seq_region_id of the seq_region it is on
+# is also returned.
+#
+# This method will also ensure that the database knows which coordinate
+# systems that this feature is stored in.
+#
+
+sub _pre_store {
+ my $self = shift;
+ my $feature = shift;
+
+ if(!ref($feature) || !$feature->isa('Bio::EnsEMBL::Feature')) {
+ throw('Expected Feature argument.');
}
- #for speed the remapping to slice may be done at the time of object creation
- my $features =
- $self->generic_fetch($constraint, $logic_name, $mapper, $slice);
-
- if(@$features && (!$features->[0]->can('contig') ||
- $features->[0]->contig == $slice)) {
- #features have been converted to slice coords already, cache and return
- return $self->{'_slice_feature_cache'}{$key} = $features;
+ $self->_check_start_end_strand($feature->start(),$feature->end(),
+ $feature->strand());
+
+ my $slice_adaptor = $self->db->get_SliceAdaptor();
+ my $slice = $feature->slice();
+
+ if(!ref($slice) || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw('Feature must be attached to Slice to be stored.');
+ }
+
+ # make sure that the feature coordinates are relative to
+ # the start of the entire seq_region
+ if($slice->start != 1 || $slice->strand != 1) {
+ #move the feature onto a slice of the entire seq_region
+ $slice = $slice_adaptor->fetch_by_region($slice->coord_system->name(),
+ $slice->seq_region_name(),
+ undef, #start
+ undef, #end
+ undef, #strand
+ $slice->coord_system->version());
+
+ $feature = $feature->transfer($slice);
+
+ if(!$feature) {
+ throw('Could not transfer Feature to slice of ' .
+ 'entire seq_region prior to storing');
+ }
+ }
+
+ #
+ # Ensure that this type of feature is known to be stored in this coord
+ # system.
+ #
+ my $csa = $self->db->get_CoordSystemAdaptor();
+ my $cs = $slice->coord_system;
+
+ my ($tab) = $self->_tables();
+ my $tabname = $tab->[0];
+
+ $csa->add_feature_table($cs, $tabname);
+
+ my $seq_region_id = $slice_adaptor->get_seq_region_id($slice);
+
+ if(!$seq_region_id) {
+ throw('Feature is associated with seq_region which is not in this DB.');
+ }
+
+ return ($feature, $seq_region_id);
+}
+
+
+#
+# helper function used to validate start/end/strand and
+# hstart/hend/hstrand etc.
+#
+sub _check_start_end_strand {
+ my $self = shift;
+ my $start = shift;
+ my $end = shift;
+ my $strand = shift;
+
+ #
+ # Make sure that the start, end, strand are valid
+ #
+ if(int($start) != $start) {
+ throw("Invalid Feature start [$start]. Must be integer.");
+ }
+ if(int($end) != $end) {
+ throw("Invalid Feature end [$end]. Must be integer.");
+ }
+ if(int($strand) != $strand || $strand < -1 || $strand > 1) {
+ throw("Invalid Feature strand [$strand]. Must be -1, 0 or 1.");
+ }
+ if($end < $start) {
+ throw("Invalid Feature start/end [$start/$end]. Start must be less " .
+ "than or equal to end.");
+ }
+
+ return 1;
+}
+
+
+#
+# Given a list of features checks if they are in the correct coord system
+# by looking at the first features slice. If they are not then they are
+# converted and placed on the slice.
+#
+sub _remap {
+ my ($self, $features, $mapper, $slice) = @_;
+
+ #check if any remapping is actually needed
+ if(@$features && (!$features->[0]->isa('Bio::EnsEMBL::Feature') ||
+ $features->[0]->slice == $slice)) {
+ return $features;
}
#remapping has not been done, we have to do our own conversion from
- # raw contig coords to slice coords
+ #to slice coords
+
+ my @out;
+
+ my $slice_start = $slice->start();
+ my $slice_end = $slice->end();
+ my $slice_strand = $slice->strand();
+ my $slice_cs = $slice->coord_system();
+
+ my ($seq_region, $start, $end, $strand);
- my @out = ();
-
- my ($feat_start, $feat_end, $feat_strand);
+ my $slice_seq_region = $slice->seq_region_name();
foreach my $f (@$features) {
#since feats were obtained in contig coords, attached seq is a contig
- my $contig_id = $f->contig->dbID();
+ my $fslice = $f->slice();
+ if(!$fslice) {
+ throw("Feature does not have attached slice.\n");
+ }
+ my $fseq_region = $fslice->seq_region_name();
+ my $fcs = $fslice->coord_system();
- my ($chr_name, $start, $end, $strand) =
- $mapper->fast_to_assembly($contig_id, $f->start(),
- $f->end(),$f->strand(),"rawcontig");
+ if(!$slice_cs->equals($fcs)) {
+ #slice of feature in different coord system, mapping required
- # undefined start means gap
- next unless defined $start;
+ ($seq_region, $start, $end, $strand) =
+ $mapper->fastmap($fseq_region,$f->start(),$f->end(),$f->strand(),$fcs);
+
+ # undefined start means gap
+ next if(!defined $start);
+ } else {
+ $start = $f->start();
+ $end = $f->end();
+ $strand = $f->strand();
+ $seq_region = $f->slice->seq_region_name();
+ }
+
+ # maps to region outside desired area
+ next if ($start > $slice_end) || ($end < $slice_start) || ($slice_seq_region ne $seq_region);
- # maps to region outside desired area
- next if ($start > $slice_end) || ($end < $slice_start);
-
#shift the feature start, end and strand in one call
if($slice_strand == -1) {
$f->move( $slice_end - $end + 1, $slice_end - $start + 1, $strand * -1 );
} else {
$f->move( $start - $slice_start + 1, $end - $slice_start + 1, $strand );
}
-
- $f->contig($slice);
-
+
+ $f->slice($slice);
+
push @out,$f;
}
-
- #update the cache
- return $self->{'_slice_feature_cache'}{$key} = \@out;
+
+ return \@out;
+}
+
+
+#
+# Given a logic name and an existing constraint this will
+# add an analysis table constraint to the feature. Note that if no
+# analysis_id exists in the columns of the primary table then no
+# constraint is added at all
+#
+sub _logic_name_to_constraint {
+ my $self = shift;
+ my $constraint = shift;
+ my $logic_name = shift;
+
+ return $constraint if(!$logic_name);
+
+ #make sure that an analysis_id exists in the primary table
+ my ($prim_tab) = $self->_tables();
+ my $prim_synonym = $prim_tab->[1];
+
+ my $found_analysis=0;
+ foreach my $col ($self->_columns) {
+ my ($syn,$col_name) = split(/\./,$col);
+ next if($syn ne $prim_synonym);
+ if($col_name eq 'analysis_id') {
+ $found_analysis = 1;
+ last;
+ }
+ }
+
+ if(!$found_analysis) {
+ warning("This feature is not associated with an analysis.\n" .
+ "Ignoring logic_name argument = [$logic_name].\n");
+ return $constraint;
+ }
+
+ my $aa = $self->db->get_AnalysisAdaptor();
+ my $an = $aa->fetch_by_logic_name($logic_name);
+
+ if(!$an) {
+ warning("No analysis exists with logic_name = [$logic_name].\n" .
+ "Returning empty list.\n");
+ return undef;
+ }
+
+ my $an_id = $an->dbID();
+
+ $constraint .= ' AND' if($constraint);
+ $constraint .= " ${prim_synonym}.analysis_id = $an_id";
+ return $constraint;
}
@@ -487,7 +750,7 @@ sub fetch_all_by_Slice_constraint {
sub store{
my $self = @_;
- $self->throw("Abstract method store not defined by implementing subclass\n");
+ throw("Abstract method store not defined by implementing subclass\n");
}
@@ -503,8 +766,8 @@ sub store{
feature to be removed from the database a dbID value must
be returned.
Returntype : none
- Exceptions : thrown if $feature arg does not implement dbID(), or if
- $feature->dbID is not a true value
+ Exceptions : thrown if $feature arg does not implement dbID(), or if
+ $feature->dbID is not a true value
Caller : general
=cut
@@ -513,13 +776,12 @@ sub store{
sub remove {
my ($self, $feature) = @_;
- unless($feature->can('dbID')) {
- $self->throw("Feature [$feature] does not implement method dbID");
+ if(!$feature || !ref($feature) || !$feature->isa('Bio::EnsEMBL::Feature')) {
+ throw('Feature argument is required');
}
- unless($feature->dbID) {
- $self->warn("BaseFeatureAdaptor::remove - dbID not defined - " .
- "feature could not be removed");
+ if(!$feature->is_stored($self->db)) {
+ throw("This feature is not stored in this database");
}
my @tabs = $self->_tables;
@@ -528,218 +790,257 @@ sub remove {
my $sth = $self->prepare("DELETE FROM $table WHERE ${table}_id = ?");
$sth->execute($feature->dbID());
- #unset the feature dbID
- $feature->dbID('');
-
+ #unset the feature dbID ad adaptor
+ $feature->dbID(undef);
+ $feature->adaptor(undef);
+
return;
}
+=head2 remove_by_Slice
-=head2 remove_by_RawContig
-
- Arg [1] : Bio::Ensembl::RawContig $contig
- Example : $feature_adaptor->remove_by_RawContig($contig);
- Description: This removes features from the database which lie on a removed
- contig. The table the features are removed from is defined by
- the abstract method_tablename, and the primary key of the table
- is assumed to be contig_id.
+ Arg [1] : Bio::Ensembl::Slice $slice
+ Example : $feature_adaptor->remove_by_RawContig($slice);
+ Description: This removes features from the database which lie on a region
+ represented by the passed in slice. Only features which are
+ fully contained by the slice are deleted; features which overlap
+ the edge of the slice are not removed.
+ The table the features are removed from is defined by
+ the abstract method_tablename.
Returntype : none
- Exceptions : thrown if no contig is supplied
+ Exceptions : thrown if no slice is supplied
Caller : general
=cut
-sub remove_by_RawContig {
- my ($self, $contig) = @_;
+sub remove_by_Slice {
+ my ($self, $slice) = @_;
- unless($contig) {
- $self->throw("BaseFeatureAdaptor::remove - no contig supplied: ".
- "Deletion of features failed.");
+ if(!$slice || !ref($slice) || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw("Slice argument is required");
}
my @tabs = $self->_tables;
-
my ($table_name) = @{$tabs[0]};
- my $sth = $self->prepare("DELETE FROM $table_name
- WHERE contig_id = ?");
+ my $seq_region_id = $self->db->get_SliceAdaptor->get_seq_region_id($slice);
+ my $start = $slice->start();
+ my $end = $slice->end();
- $sth->execute($contig->dbID);
+ #
+ # Delete only features fully on the slice, not overlapping ones
+ #
+ my $sth = $self->prepare("DELETE FROM $table_name " .
+ "WHERE seq_region_id = ? " .
+ "AND seq_region_start >= ? " .
+ "AND seq_region_end <= ?");
- return;
+ $sth->execute($seq_region_id, $start, $end);
+ $sth->finish();
}
-=head2 _tables
- Args : none
- Example : $tablename = $self->_table_name()
- Description: ABSTRACT PROTECTED Subclasses are responsible for implementing
- this method. It should list of [tablename, alias] pairs.
- Additionally the primary table (with the dbID, analysis_id, and
- score) should be the first table in the list.
- e.g:
- ( ['repeat_feature', 'rf'],
- ['repeat_consensus', 'rc']);
- used to obtain features.
- Returntype : list of [tablename, alias] pairs
- Exceptions : thrown if not implemented by subclass
- Caller : BaseFeatureAdaptor::generic_fetch
-=cut
+#_tables
+#
+# Args : none
+# Example : $tablename = $self->_table_name()
+# Description: ABSTRACT PROTECTED Subclasses are responsible for implementing
+# this method. It should list of [tablename, alias] pairs.
+# Additionally the primary table (with the dbID, analysis_id, and
+# score) should be the first table in the list.
+# e.g:
+# ( ['repeat_feature', 'rf'],
+# ['repeat_consensus', 'rc']);
+# used to obtain features.
+# Returntype : list of [tablename, alias] pairs
+# Exceptions : thrown if not implemented by subclass
+# Caller : BaseFeatureAdaptor::generic_fetch
+#
sub _tables {
my $self = shift;
- $self->throw("abstract method _tables not defined by implementing" .
+ throw("abstract method _tables not defined by implementing" .
" subclass of BaseFeatureAdaptor");
return undef;
}
-=head2 _columns
-
- Args : none
- Example : $tablename = $self->_columns()
- Description: ABSTRACT PROTECTED Subclasses are responsible for implementing
- this method. It should return a list of columns to be used
- for feature creation
- Returntype : list of strings
- Exceptions : thrown if not implemented by subclass
- Caller : BaseFeatureAdaptor::generic_fetch
-
-=cut
+#_columns
+#
+# Args : none
+# Example : $tablename = $self->_columns()
+# Description: ABSTRACT PROTECTED Subclasses are responsible for implementing
+# this method. It should return a list of columns to be used
+# for feature creation
+# Returntype : list of strings
+# Exceptions : thrown if not implemented by subclass
+# Caller : BaseFeatureAdaptor::generic_fetch
+#
sub _columns {
my $self = shift;
- $self->throw("abstract method _columns not defined by implementing" .
+ throw("abstract method _columns not defined by implementing" .
" subclass of BaseFeatureAdaptor");
}
-=head2 _left_join
+# _default_where_clause
+#
+# Arg [1] : none
+# Example : none
+# Description: May be overridden to provide an additional where constraint to
+# the SQL query which is generated to fetch feature records.
+# This constraint is always appended to the end of the generated
+# where clause
+# Returntype : string
+# Exceptions : none
+# Caller : generic_fetch
+#
- Arg [1] : none
- Example :
- Description:
- Returntype :
- Exceptions :
- Caller :
+sub _default_where_clause {
+ my $self = shift;
-=cut
+ return '';
+}
+# _left_join
+# Arg [1] : none
+# Example : none
+# Description: Can be overridden by a subclass to specify any left joins
+# which should occur. The table name specigfied in the join
+# must still be present in the return values of
+# Returntype : a {'tablename' => 'join condition'} pair
+# Exceptions : none
+# Caller : general
+#
-=head2 _default_where_clause
+sub _left_join {
+ my $self = shift;
- Arg [1] : none
- Example : none
- Description: May be overridden to provide an additional where constraint to
- the SQL query which is generated to fetch feature records.
- This constraint is always appended to the end of the generated
- where clause
- Returntype : string
- Exceptions : none
- Caller : generic_fetch
+ return ();
+}
-=cut
-sub _default_where_clause {
+
+#_final_clause
+
+# Arg [1] : none
+# Example : none
+# Description: May be overriden to provide an additional clause to the end
+# of the SQL query used to fetch feature records.
+# This is useful to add a required ORDER BY clause to the
+# query for example.
+# Returntype : string
+# Exceptions : none
+# Caller : generic_fetch
+
+sub _final_clause {
my $self = shift;
return '';
}
+#_objs_from_sth
-=head2 _left_join
+# Arg [1] : DBI::row_hashref $hashref containing key-value pairs
+# for each of the columns specified by the _columns method
+# Example : my @feats = $self->_obj_from_hashref
+# Description: ABSTRACT PROTECTED The subclass is responsible for implementing
+# this method. It should take in a DBI row hash reference and
+# return a list of created features in contig coordinates.
+# Returntype : list of Bio::EnsEMBL::*Features in contig coordinates
+# Exceptions : thrown if not implemented by subclass
+# Caller : BaseFeatureAdaptor::generic_fetch
- Arg [1] : none
- Example : none
- Description: Can be overridden by a subclass to specify any left joins
- which should occur. The table name specigfied in the join
- must still be present in the return values of
- Returntype : a {'tablename' => 'join condition'} pair
- Exceptions : none
- Caller : general
+sub _objs_from_sth {
+ my $self = shift;
-=cut
+ throw("abstract method _obj_from_sth not defined by implementing"
+ . " subclass of BaseFeatureAdaptor");
+}
-sub _left_join {
+# deleteObj
+#
+# Arg [1] : none
+# Example : none
+# Description: Cleans up internal caches and references to other objects so
+# that correct garbage collection may occur.
+# Returntype : none
+# Exceptions : none
+# Caller : Bio::EnsEMBL::DBConnection::deleteObj
+
+
+sub deleteObj {
my $self = shift;
- return '';
+ #flush feature cache
+ %{$self->{'_slice_feature_cache'}} = ();
}
-=head2 _final_clause
- Arg [1] : none
- Example : none
- Description: May be overriden to provide an additional clause to the end
- of the SQL query used to fetch feature records.
- This is useful to add a required ORDER BY clause to the
- query for example.
- Returntype : string
- Exceptions : none
- Caller : generic_fetch
+
+=head2 fetch_all_by_RawContig_constraint
+
+ Description: DEPRECATED use fetch_all_by_RawContig_constraint instead
=cut
-sub _final_clause {
+sub fetch_all_by_RawContig_constraint {
my $self = shift;
-
- return '';
+ deprecate('Use fetch_all_by_Slice_constraint() instead.');
+ return $self->fetch_all_by_slice_constraint(@_);
}
-=head2 _objs_from_sth
+=head2 fetch_all_by_RawContig
- Arg [1] : DBI::row_hashref $hashref containing key-value pairs
- for each of the columns specified by the _columns method
- Example : my @feats = $self->_obj_from_hashref
- Description: ABSTRACT PROTECTED The subclass is responsible for implementing
- this method. It should take in a DBI row hash reference and
- return a list of created features in contig coordinates.
- Returntype : list of Bio::EnsEMBL::*Features in contig coordinates
- Exceptions : thrown if not implemented by subclass
- Caller : BaseFeatureAdaptor::generic_fetch
+ Description: DEPRECATED use fetch_all_by_Slice instead
=cut
-sub _objs_from_sth {
+sub fetch_all_by_RawContig {
my $self = shift;
+ deprecate('Use fetch_all_by_Slice() instead.');
+ return $self->fetch_all_by_Slice(@_);
+}
- $self->throw("abstract method _obj_from_sth not defined by implementing"
- . " subclass of BaseFeatureAdaptor");
-}
+=head2 fetch_all_by_RawContig_and_score
+ Description: DEPRECATED use fetch_all_by_Slice_and_score instead
-=head2 deleteObj
+=cut
- Arg [1] : none
- Example : none
- Description: Cleans up internal caches and references to other objects so
- that correct garbage collection may occur.
- Returntype : none
- Exceptions : none
- Caller : Bio::EnsEMBL::DBConnection::deleteObj
+sub fetch_all_by_RawContig_and_score{
+ my $self = shift;
+ deprecate('Use fetch_all_by_Slice_and_score() instead.');
+ return $self->fetch_all_by_Slice_and_score(@_);
+}
+
+=head2 remove_by_RawContig
+
+ Description: DEPRECATED use remove_by_Slice instead
=cut
-sub deleteObj {
+sub remove_by_RawContig {
my $self = shift;
-
- #flush feature cache
- %{$self->{'_slice_feature_cache'}} = ();
+ deprecate("Use remove_by_Slice instead");
+ return $self->remove_by_Slice(@_);
}
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/ChromosomeAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/ChromosomeAdaptor.pm
index e8bb7c2463..35b19351f5 100644
--- a/modules/Bio/EnsEMBL/DBSQL/ChromosomeAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/ChromosomeAdaptor.pm
@@ -11,16 +11,12 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::ChromosomeAdaptor - DB Connectivity for Chromosome Object
-
-=head1 SYNOPSIS
-
-$chromosome_adaptor = $db_adaptor->get_ChromosomeAdaptor();
-$chromosome = $chromosome_adaptor->fetch_by_chr_name('12');
+Bio::EnsEMBL::DBSQL::ChromosomeAdaptor - DEPRECATED
+use Bio::EnsEMBL::SliceAdator instead
=head1 DESCRIPTION
-This is a database adaptor used to retrieve chromosome objects from a database.
+This class is deprecated. SliceAdaptor should be used instead.
=head1 AUTHOR - Ewan Birney
@@ -34,148 +30,91 @@ package Bio::EnsEMBL::DBSQL::ChromosomeAdaptor;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
+use Bio::EnsEMBL::DBSQL::SliceAdaptor;
use Bio::EnsEMBL::Chromosome;
-@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+@ISA = qw(Bio::EnsEMBL::DBSQL::SliceAdaptor);
+
=head2 fetch_by_dbID
- Arg [1] : int $id
- unique database identifier for chromosome to retrieve
- Example : my $chromosome = $chromosome_adaptor->fetch_by_dbID(1);
- Description: Retrieves a Chromosome object from the database using its
- unique identifier. Note the the identifier is the dbID and
- does NOT correspond to the chromosome name. dbID 1 does NOT
- necessarily correspond to chromosome '1'
- Returntype : Bio::EnsEMBL::Chromosome
- Exceptions : thrown if $id not defined
- Caller : general
+ Description: DEPRECATED Use SliceAdaptor::fetch_by_seq_region_id instead
=cut
sub fetch_by_dbID {
my ($self,$id) = @_;
- $self->throw("Chromosome dbID argument required\n") unless defined $id;
-
- unless(defined $self->{'_chr_cache'} ) {
- $self->{'_chr_cache'} = {};
- $self->{'_chr_name_cache'} = {};
- }
-
- # If there is not already a cached version of this chromosome pull it
- # from the database and add it to the cache.
- unless( $self->{'_chr_cache'}->{$id} ) {
- my $sth;
+ deprecate('Use SliceAdaptor::fetch_by_seq_region_id instead.');
- $sth = $self->prepare( "SELECT chromosome_id, name, length
- FROM chromosome
- WHERE chromosome_id = ?" );
- $sth->execute( $id );
+ my $chr = $self->SUPER::fetch_by_seq_region_id($id);
- my $a = $sth->fetchrow_arrayref();
- $self->throw("Could determine chromosome name from dbID $id\n") unless $a;
- $self->_create_object_from_arrayref( $a );
- }
-
- return $self->{'_chr_cache'}->{$id};
+ #change blessing to chromosome and reset adaptor
+ bless $chr, 'Bio::EnsEMBL::Chromosome';
+ $chr->adaptor($self);
+ return $chr;
}
=head2 fetch_by_chr_name
- Arg [1] : string $chr_name
- the name of the chromosome to retrieve
- Example : $chromosome = $chromosome_adaptor->fetch_by_chr_name('X');
- Description: Retrieves a chromosome object from the database using its name.
- Returntype : Bio::EnsEMBL::Chromosome
- Exceptions : none
- Caller : general
+ Description: DEPRECATED Use SliceAdaptor::fetch_by_region instead
=cut
sub fetch_by_chr_name{
my ($self,$chr_name) = @_;
- unless(defined $self->{'_chr_cache'} ) {
- $self->{'_chr_cache'} = {};
- $self->{'_chr_name_cache'} = {};
- }
-
- unless($self->{'_chr_name_cache'}->{lc($chr_name)}) {
- my $sth;
-
- $sth = $self->prepare( "SELECT chromosome_id, name, length
- FROM chromosome WHERE name = ?" );
- $sth->execute( "$chr_name" );
+ deprecate('Use SliceAdaptor::fetch_by_region instead.');
- my $a = $sth->fetchrow_arrayref();
+ my $top_level = $self->db->get_CoordSystemAdaptor->fetch_top_level();
- $self->throw("Do not recognise chromosome $chr_name\n") unless $a;
-
- $self->_create_object_from_arrayref( $a );
- }
- return $self->{'_chr_name_cache'}->{lc($chr_name)};
+ my $chr = $self->SUPER::fetch_by_region($top_level->name(),
+ $chr_name,
+ undef,
+ undef,
+ undef,
+ $top_level->version);
+ #change blessing and adaptor
+ bless $chr, 'Bio::EnsEMBL::Chromosome';
+ $chr->adaptor($self);
+ return $chr;
}
=head2 fetch_all
- Args : none
- Example : @chromosomes = $chromosome_adaptor->fetch_all();
- Description: Retrieves every chromosome object from the database.
- Returntype : listref of Bio::EnsEMBL::Chromosome
- Exceptions : none
- Caller : general
+ Description: DEPRECATED Use SliceAdaptor::fetch_all instead
=cut
sub fetch_all {
my($self) = @_;
- my @chrs = ();
-
- my $sth = $self->prepare( "SELECT chromosome_id, name, length
- FROM chromosome");
- $sth->execute();
- while( my $a = $sth->fetchrow_arrayref() ) {
- push @chrs, $self->_create_object_from_arrayref( $a );
- }
- return \@chrs;
-}
-=head2 _create_object_from_arrayref
+ deprecate('Use SliceAdaptor::fetch_all instead.');
- Args : array ref containing a row of the chromosome table
- Example : $self->_create_object_from_arrayref
- Description: Creates object from array reference
- Returntype : Bio::Chromosome object
- Exceptions : none
- Caller : general
+ my $top_level = $self->db->get_CoordSystemAdaptor->fetch_top_level();
+ my $chrs = $self->SUPER::fetch_all($top_level->name, $top_level->version);
-=cut
+ foreach my $chr (@$chrs) {
+ bless $chr, 'Bio::EnsEMBL::Chromosome';
+ $chr->adaptor($self);
+ }
-sub _create_object_from_arrayref {
- my( $self,$a ) =@_;
- my $chr = new Bio::EnsEMBL::Chromosome(
- -adaptor => $self, -dbID => $a->[0],
- -chr_name => $a->[1], -length => $a->[2]
- );
- return $self->{'_chr_cache' }->{$a->[0]} =
- $self->{'_chr_name_cache'}->{lc($a->[1])} = $chr ;
+ return $chrs;
}
+
+=head2 store
+
+ Description: DEPRECATED no replacement exists
+
+=cut
+
sub store{
- my ($self, $chromosome) = @_;
-
- $self->throw("can't store a chromosome without a name")
- unless my $chr_name = $chromosome->chr_name;
- $self->throw("can't store a chromosome without a length")
- unless my $length = $chromosome->length;
- my $sth = $self->db->prepare("insert into chromosome set name=?, length=?");
- $sth->execute("$chr_name", $length);
- $chromosome->dbID($sth->{'mysql_insertid'});
- $chromosome->adaptor($self);
+ ### XXX Should there be a replacement for this?
+ deprecate('No replacement has been implemented.');
}
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/CloneAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/CloneAdaptor.pm
index 31d8b314f3..baaf68829e 100644
--- a/modules/Bio/EnsEMBL/DBSQL/CloneAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/CloneAdaptor.pm
@@ -7,22 +7,15 @@
#
# You may distribute this module under the same terms as perl itself
-# POD documentation - main docs before the code
-
=head1 NAME
-Bio::EnsEMBL::DBSQL::CloneAdaptor
-
-=head1 SYNOPSIS
-
- $clone_adaptor = $database_adaptor->get_CloneAdaptor();
- $clone = $clone_adaptor->fetch_by_dbID(1234);
- @contig = $clone->get_all_Contigs();
- @genes = $clone->get_all_Genes();
+Bio::EnsEMBL::DBSQL::CloneAdaptor - DEPRECATED use Bio::EnsEMBL::SliceAdaptor
+instead
=head1 DESCRIPTION
-Database adaptor for the creation of clone objects
+DEPRECATED - Use Bio::EnsEMBL::DBSQL::SliceAdaptor to create slices on clones
+instead
=head1 AUTHOR - Ewan Birney
@@ -30,421 +23,179 @@ This modules is part of the Ensembl project http://www.ensembl.org
Email birney@ebi.ac.uk
-=head1 APPENDIX
-
-The rest of the documentation details each of the object methods. Internal
-methods are usually preceded with a _
-
=cut
-# Let the code begin...
-
package Bio::EnsEMBL::DBSQL::CloneAdaptor;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::DBSQL::SliceAdaptor;
use Bio::EnsEMBL::Clone;
+use Bio::EnsEMBL::Utils::Exception qw( deprecate throw warning );
+@ISA = qw(Bio::EnsEMBL::DBSQL::SliceAdaptor);
-@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+=head2 new
+ Description: DEPRECATED. Use Bio::EnsEMBL::DBSQL::SliceAdaptor instead
-=head2 _generic_sql_fetch
+=cut
- Arg [1] : string $where_clause
- the WHERE clause of the SQL query to be executed
- Example : $clone = $self->_generic_sql_fetch('embl_acc = AC011082');
- Description: PRIVATE Performs an SQL query and returns an Clone object
- created from the result.
- Returntype : Bio::EnsEMBL::Clone
- Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::CloneAdaptor
+sub new {
+ my $class = shift;
-=cut
+ deprecate("Bio::EnsEMBL::DBSQL::CloneAdaptor is a deprecated class\n" .
+ "Use Bio::EnsEMBL::DBSQL::SliceAdaptor instead");
-sub _generic_sql_fetch {
- my( $self, $where_clause ) = @_;
-
- my $sql = q{
- SELECT clone_id
- , name
- , embl_acc
- , version
- , embl_version
- , htg_phase
- , UNIX_TIMESTAMP(created)
- , UNIX_TIMESTAMP(modified)
- FROM clone }
- . $where_clause .
- q{ ORDER BY embl_version DESC };
-
- #print STDERR "issue: $sql\n";
-
- my $sth = $self->prepare($sql);
- $sth->execute;
-
- if (my @fields = $sth->fetchrow) {
- return Bio::EnsEMBL::Clone->new($self, @fields);
- } else {
- return;
- }
+ return $class->SUPER::new(@_);
}
=head2 fetch_by_accession
- Arg [1] : string $acc
- the EMBL accession for the clone
- Example : $clone = $clone_adaptor->fetch_by_accession('AC011082');
- Description: fetches a clone by its EMBL/GenBank accession number
- It will fetch the highest version in the database
- Returntype : Bio::EnsEMBL::Clone
- Exceptions : thrown if $acc not defined or if no clone with accession $acc
- exists in the database
- Caller : general
+ Description: DEPRECATED. Use SliceAdaptor::fetch_by_region instead
=cut
-sub fetch_by_accession {
- my ($self,$acc) = @_;
-
- unless ($acc) {
- $self->throw("Accession not given");
- }
+sub fetch_by_accession {
+ my ($self,$acc) = @_;
- my $clone = $self->_generic_sql_fetch(
- qq{ WHERE embl_acc = '$acc' }
- );
+ deprecate('Use SliceAdaptor::fetch_by_region instead');
- if ($clone) {
- return $clone;
- } else {
- $self->throw("No clone with accession '$acc'");
+ #this unfortunately needs a version on the end to work
+ if(! ($acc =~ /\./)) {
+ my $sth = $self->prepare("SELECT sr.name " .
+ "FROM seq_region sr, coord_system cs " .
+ "WHERE cs.name = 'clone' " .
+ "AND cs.coord_system_id = sr.coord_system_id ".
+ "AND sr.name LIKE '$acc.%'");
+ $sth->execute();
+ if(!$sth->rows()) {
+ $sth->finish();
+ throw("Clone $acc not found in database");
}
+
+ ($acc) = $sth->fetchrow_array();
+
+ $sth->finish();
+ }
+
+ my $clone = $self->fetch_by_region('clone', $acc);
+
+ #rebless slice into clone so old methods still work
+ return bless($clone, 'Bio::EnsEMBL::Clone');
}
=head2 fetch_by_accession_version
- Arg [1] : string $acc
- the EMBL accession number
- Arg [2] : int $ver
- the EMBL accession version
- Example : $clone = $clone_adaptor->fetch_by_accession_version($acc, $ver);
- Description: retrieves a Clone object with accession $acc and version $ver
- from the database.
- Returntype : Bio::EnsEMBL::Clone
- Exceptions : thrown if $acc or $ver is not defined or if no clone exists with
- in the database with accession $acc and version $ver
- Caller : general
+ Description: DEPRECATED. Use SliceAdaptor::fetch_by_region instead
=cut
sub fetch_by_accession_version {
my ($self, $acc, $ver) = @_;
-
- unless ($acc and $ver) {
- $self->throw("Need both accession (got '$acc') and version (got '$ver')");
- }
- my $clone =
- $self->_generic_sql_fetch( qq{ WHERE embl_acc = '$acc'
- AND embl_version = '$ver' });
- if ($clone) {
- return $clone;
- } else {
- $self->throw("no clone with accession '$acc' and version '$ver'");
- }
+ deprecate('Use SliceAdaptor::fetch_by_region instead');
+
+ my $clone = $self->fetch_by_region('clone', "$acc.$ver");
+
+ #rebless slice into clone so old methods still work
+ return bless($clone, 'Bio::EnsEMBL::Clone');
}
=head2 fetch_by_name
- Arg [1] : string $name
- the "name" of the clone.
- Example : $clone = $clone_adaptor->fetch_by_name($name);
- Description: fetches a single clone by the "name" field. The name
- field is often the EMBL ID of a clone (which itself is often
- the same as the accession number) but is a deliberately open
- slot for another identifier for the clone, eg. the stringified
- tracking identifier in a sequencing centre
- Returntype : Bio::EnsEMBL::Clone
- Exceptions : thrown if name is a non-true value (i.e. undefined or '') or
- if there is no clone with name $name in the database
- Caller : general
+ Description: DEPRECATED. Use SliceAdaptor::fetch_by_region instead
=cut
sub fetch_by_name {
my ($self, $name) = @_;
-
- $self->throw("name not given") unless $name;
-
- my $clone = $self->_generic_sql_fetch(qq{ WHERE name = '$name' });
-
- if ($clone) {
- return $clone;
- } else {
- $self->throw("No Clone with name '$name'");
- }
+ deprecate('Use SliceAdaptor::fetch_by_region instead');
+ return $self->fetch_by_accession($name);
}
=head2 fetch_by_dbID
- Arg [1] : int $id
- the numeric internal id of the clone table
- Example : $clone = $clone_adaptor->fetch_by_dbID(7);
- Description: fetches a clone by the internal id in the database
- of the Clone. Most likely this will be used by
- other adaptors to build objects from references to Clones
- Returntype : Bio::EnsEMBL::Clone
- Exceptions : thrown if $id is not provided
- Caller : general
+ Description: DEPRECATED. Use SliceAdaptor::fetch_by_seq_region_id instead
=cut
sub fetch_by_dbID {
- my ($self,$id) = @_;
-
- if( !defined $id ) {
- $self->throw("No internal ID provided");
- }
+ my ($self,$id) = @_;
- my $clone = $self->_generic_sql_fetch("WHERE clone_id = $id");
+ my $clone = $self->fetch_by_seq_region_id($id);
- return $clone;
+ return bless $clone, 'Bio::EnsEMBL::Clone';
}
=head2 fetch_all
- Arg [1] : none
- Example : @clones = $clone_adaptor->fetch_all();
- Description: Retrieves every clone from the database.
- Returntype : listref of Bio::EnsEMBL::Clone
- Exceptions : none
- Caller : none
+ Description: DEPRECATED. Use SliceAdaptor::fetch_all instead
=cut
sub fetch_all {
my $self = shift;
- my $sth = $self->prepare("SELECT clone_id, name, embl_acc, version,
- embl_version, htg_phase,
- UNIX_TIMESTAMP(created), UNIX_TIMESTAMP(modified)
- FROM clone");
-
- $sth->execute();
+ my $clones = $self->SUPER::fetch_all('clone');
- my ($clone_id, $name, $embl_acc, $version, $embl_version, $htg_phase,
- $created, $modified);
+ map {bless $_, 'Bio::EnsEMBL::Clone'} @$clones;
- $sth->bind_columns(\$clone_id, \$name, \$embl_acc, \$version, \$embl_version,
- \$htg_phase, \$created, \$modified);
-
- my @clones;
-
- while($sth->fetch()) {
- push @clones, new Bio::EnsEMBL::Clone($self, $clone_id, $name, $embl_acc,
- $version, $embl_version, $htg_phase,
- $created, $modified);
- }
-
- return \@clones;
+ return $clones;
}
=head2 list_embl_version_by_accesssion
- Arg [1] : string $id
- the EMBL accession of the clone versions to retrieve
- Example : @vers = $clone->list_embl_version_by_accession($accession)
- Description: Returns a list of versions for a given EMBL accession
- Returntype : listref of ints
- Exceptions : thrown if $id arg is not defined or if no clone with accession
- $id exists in the database
- Caller : general
+ Description: DEPRECATED There is currently no replacement for this method
+ Perhaps there should be?
=cut
sub list_embl_version_by_accession {
- my ($self,$id) = @_;
- my (@vers);
-
- if( !defined $id) {$self->throw("Don't have $id for new adaptor");}
+ deprecate('There is currently no replacement for this method');
- my $sth = $self->prepare(qq{
- SELECT distinct embl_version
- FROM clone
- WHERE embl_acc = '$id'
- });
- my $res = $sth ->execute();
-
- while( my $rowhash = $sth->fetchrow_hashref) {
- push @vers, $rowhash->{'embl_version'};
- }
- $sth->finish;
-
- $self->throw("no clone $id") unless scalar @vers > 0;
-
- return \@vers;
+ ### XXX Perhaps there should be a replacement?
+ return ();
}
=head2 remove
- Arg [1] : Bio::Ensembl::Clone $clone
- Example : $clone_adaptor->remove($clone);
- Description: Deletes clone (itself), including contigs and features,
- but not its genes
- Returntype : none
- Exceptions : thrown if the clone deletion fails
- throw if attached to the wrong database
- Caller : ?
+ Description: DEPRECATED There is currently no replacement for this method
+ Perhaps there should be?
+
=cut
sub remove {
- my ($self, $clone) = @_;
-
- if ($clone->adaptor ne $self) {
- $self->throw("Trying to delete a clone attached to a different database.");
- }
+ deprecate('There is currently no replacement for this method');
- # Delete all contigs and related features
- my $contigs = $clone->get_all_Contigs;
- my $rca = $self->db->get_RawContigAdaptor;
-
- foreach my $contig ( @$contigs ) {
- $rca->remove($contig);
- }
-
- # Delete the row for the clone
- my $sth = $self->prepare("DELETE FROM clone WHERE clone_id = ?");
- $sth->execute($clone->dbID);
- $self->throw("Failed to delete clone for clone_id '$clone->dbID'")
- unless $sth->rows;
+ ### XXX Perhaps there should be a replacement?
+ return 0;
}
-=head2 get_all_Genes
-
- Arg [1] : string $clone_id
- the EMBL accession for the clone from which the genes are to be
- retrieved
- Example : my @genes = $clone_adaptor->get_all_Genes('AC011082');
- Description: Retrieves a list of Gene objects which are present on a clone.
- It would be better to have this on the gene adaptor but
- for now it will stay here.
- Returntype : listref of Bio::EnsEMBL::Genes
- Exceptions : thrown if $clone_id is not defined
- Caller : Clone::get_all_Genes
-
-=cut
-
-sub get_all_Genes {
- my ($self, $clone_id) = @_;
-
- $self->throw("clone_id not given") unless $clone_id;
-
- my $sth = $self->prepare(qq{
- SELECT t.gene_id
- FROM transcript t
- , exon_transcript et
- , exon e
- , contig c
- WHERE e.contig_id = c.contig_id
- AND et.exon_id = e.exon_id
- AND t.transcript_id = et.transcript_id
- AND c.clone_id = $clone_id
- });
- $sth->execute();
-
- my $geneAdaptor = $self->db->get_GeneAdaptor();
- my( %got, @genes );
- while (my ($gene_id) = $sth->fetchrow) {
- unless ($got{$gene_id}) {
- if (my $gene = $geneAdaptor->fetch_by_dbID($gene_id)) {
- push(@genes, $gene);
- }
- $got{$gene_id} = 1;
- }
- }
- return \@genes;
-}
-
=head2 store
- Arg [1] : Bio::EnsEMBL::Clone $clone
- the Clone to store in the database
- Example : $clone_adaptor->store($clone);
- Description: Stores a clone object and its associated contigs
- in the database and returns the dbID of the new db record
- Returntype : int
- Exceptions : thrown if $clone is not defined or if $clone is not a
- Bio::EnsEMBL::Clone or if the database insertion fails
- Caller : general
+ Description: DEPRECATED There is currently no replacement for this method
+ Perhaps there should be?
+
=cut
sub store{
- my ($self, $clone) = @_;
-
- unless($clone) {
- $self->throw("trying to write a clone without a clone object : $!\n");
- }
-
- my @contigs = @{$clone->get_all_Contigs};
-
- if( !$clone->isa('Bio::EnsEMBL::Clone') ) {
- $self->throw("Clone '$clone' is not a 'Bio::EnsEMBL::Clone'");
- }
-
- my $sql = "insert into clone(name,
- embl_acc,
- version,
- embl_version,
- htg_phase,
- created,
- modified)
- values( '".$clone->id."' , '".
- $clone->embl_id."', ".
- $clone->version.",".
- $clone->embl_version.", ".
- $clone->htg_phase.",
- FROM_UNIXTIME('".$clone->created."'),
- FROM_UNIXTIME('".$clone->modified."'))";
-
- my $sth = $self->prepare($sql);
- my $rv = $sth->execute();
- $self->throw("Failed to insert clone $clone->id") unless $rv;
-
- $sth->finish();
- $sth = $self->prepare("select last_insert_id()");
- $sth->execute;
-
- my ($id) = $sth->fetchrow();
- $self->throw("Failed to get auto-incremented ID") unless $id;
-
- $sth->finish();
-
- #update this clones database identifier
- $clone->dbID($id);
- $clone->adaptor($self);
-
- #store the contigs which were on this clone
- my $rca = $self->db->get_RawContigAdaptor();
- foreach my $contig(@contigs){
- $rca->store($contig, $clone);
- }
-
+ deprecate('There is currently no replacement for this method');
- return $id;
+ ### XXX Perhaps there should be a replacement?
+ return 0;
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/CompressedSequenceAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/CompressedSequenceAdaptor.pm
new file mode 100644
index 0000000000..6fb9690ecc
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DBSQL/CompressedSequenceAdaptor.pm
@@ -0,0 +1,186 @@
+#
+# EnsEMBL module for Bio::EnsEMBL::DBSQL::SequenceAdaptor
+#
+#
+# Copyright EMBL/EBI
+#
+# You may distribute this module under the same terms as perl itself
+
+=head1 NAME
+
+Bio::EnsEMBL::DBSQL::CompressedSequenceAdaptor - Facilitates DB storage and retrieval of compressed sequence
+
+=head1 SYNOPSIS
+
+$seq_adptr = $database_adaptor->get_SequenceAdaptor();
+$dna = ${$seq_adptr->fetch_by_Slice_start_end_strand($slice, 1, 1000, -1);}
+
+=head1 DESCRIPTION
+
+An adaptor for the retrieval of compressed DNA sequence from the EnsEMBL
+database
+
+=head1 CONTACT
+
+Post questions/comments to the EnsEMBL development list:
+ensembl-dev@ebi.ac.uk
+
+=head1 METHODS
+
+=cut
+
+package Bio::EnsEMBL::DBSQL::CompressedSequenceAdaptor;
+
+use vars qw(@ISA);
+use strict;
+
+use Bio::EnsEMBL::DBSQL::SequenceAdaptor;
+
+@ISA = qw(Bio::EnsEMBL::DBSQL::SequenceAdaptor);
+
+
+sub _fetch_seq {
+ my $self = shift;
+ my $seq_region_id = shift;
+ my $start = shift;
+ my $len = shift;
+
+ #calculate the offset and start in the compressed sequence
+ my $comp_start = ($start-1 >> 2) + 1;
+ my $comp_len = ($len >> 2) + 2;
+
+ my ($bvector, $nline);
+
+ my $sth = $self->prepare(
+ "SELECT SUBSTRING( d.sequence, ?, ?), n_line
+ FROM dnac d
+ WHERE d.seq_region_id = ?");
+ $sth->execute($comp_start, $comp_len, $seq_region_id);
+ $sth->bind_columns(\$bvector, \$nline);
+ $sth->fetch();
+ $sth->finish();
+
+ #convert sequence from binary string to 0123 string
+ my $bitlen = length($bvector) << 2;
+ my $str = '';
+ for(my $i=0; $i < $bitlen; $i++) {
+ $str .= vec($bvector, $i, 2);
+ }
+
+ #convert from 0123 to ACTG
+ $str =~ tr/0123/ACTG/;
+
+ $str = substr($str, ($start-1)%4, $len);
+
+ #expand the nlines and place them back in the sequence
+ my @nlines = split(/:/, $nline);
+ foreach my $nl (@nlines) {
+ my ($offset,$char,$nlen) = $nl =~ /(\d+)(\D)(\d+)/;
+
+ #skip nlines entirely out of range
+ next if(($offset+$nlen-1) < $start || $offset > ($start+$len-1));
+
+ #obtain relative offset into requested region
+ $offset = $offset - $start + 1;
+
+ #nlines that partially overlap requested region have to be shrunk
+ if($offset < 1) {
+ $nlen = $nlen - (1-$offset);
+ $offset = 1;
+ }
+ if($offset + $nlen > $start+$len) {
+ $nlen = $len - $offset + 1;
+ }
+
+ substr($str,$offset-1,$nlen) = $char x $nlen;
+ }
+
+ return \$str;
+}
+
+
+=head2 store
+
+ Arg [1] : string $seq_region_id the id of the sequence region this dna
+ will be associated with.
+ Arg [2] : string reference $sequence the dna sequence to be stored in
+ the database
+ Example : $dbID = $seq_adaptor->store(12,\'ACTGGGTACCAAACAAACACAACA');
+ Description: stores a dna sequence in the databases dna table and returns the
+ database identifier for the new record.
+ Returntype : int
+ Exceptions : none
+ Caller : Bio::EnsEMBL::DBSQL::RawContigAdaptor::store
+
+=cut
+
+sub store {
+ my ($self, $seq_region_id, $sequence) = @_;
+
+ if(!$seq_region_id) {
+ throw('seq_region_id is required');
+ }
+
+ $sequence = uc($sequence);
+
+ my $bvector;
+
+ #convert sequence to 0s,1s,2s and 3s
+ $sequence =~ tr/ACTG/0123/;
+
+ #nlines cover sequence which is not ACTG such as N
+ #nline format is a set of colon delimited int, char, int triplets:
+ #<offset><code><length>
+ my($nline_char,$nline_len,$nline_off);
+ my @nlines;
+
+ my $len = length($sequence);
+ for(my $i=0; $i < $len; $i++) {
+ #pack the
+ my $char = substr($sequence,$i,1);
+
+ if(($char+0) eq $char) { #fast way to check if an int
+ vec($bvector, $i,2) = $char;
+ if($nline_char) {
+ #end of an nline
+ push @nlines, "$nline_off$nline_char$nline_len";
+ $nline_char = undef;
+ $nline_len = 0;
+ $nline_off = 0;
+ }
+ } else {
+ #this was not an ACTG
+ if($nline_char) {
+ if($nline_char eq $char) {
+ #continuation of an nline
+ $nline_len++;
+ } else {
+ #end of a previous nline and start of a new one
+ push @nlines, "$nline_off$nline_char$nline_len";
+ $nline_char = $char;
+ $nline_len = 1;
+ $nline_off = $i+1;
+ }
+ } else {
+ #start of a new nline
+ $nline_char = $char;
+ $nline_len = 1;
+ $nline_off = $i+1;
+ }
+ }
+
+ vec($bvector, $i,2) = $char;
+ }
+
+ my $nline = join(':', @nlines);
+ my $statement = $self->prepare(
+ "INSERT INTO dnac(seq_region_id, sequence, n_line) VALUES(?,?,?)");
+
+ $statement->execute($seq_region_id, $bvector, $nline);
+
+ $statement->finish();
+ return;
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/CoordSystemAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/CoordSystemAdaptor.pm
new file mode 100644
index 0000000000..fb109de1bc
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DBSQL/CoordSystemAdaptor.pm
@@ -0,0 +1,902 @@
+#
+# EnsEMBL module for Bio::EnsEMBL::DBSQL::CoordSystemAdaptor
+#
+#
+
+=head1 NAME
+
+Bio::EnsEMBL::DBSQL::CoordSystemAdaptor
+
+=head1 SYNOPSIS
+
+ my $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+
+ my $csa = $db->get_CoordSystemAdaptor();
+
+ #
+ # Get all coord systems in the database:
+ #
+ foreach my $cs (@{$csa->fetch_all()}) {
+ print $cs->name, ' ', $cs->version, "\n";
+ }
+
+ #
+ # Fetching by name:
+ #
+
+ #use the default version of coord_system 'chromosome' (e.g. NCBI33):
+ $cs = $csa->fetch_by_name('chromosome');
+
+ #get an explicit version of coord_system 'chromosome':
+ $cs = $csa->fetch_by_name('chromsome', 'NCBI34');
+
+ #get all coord_systems of name 'chromosome':
+ foreach $cs (@{$csa->fetch_all_by_name('chromosome')}) {
+ print $cs->name, ' ', $cs->version, "\n";
+ }
+
+ #
+ # Fetching by top level:
+ #
+
+ #Get the default top_level coord system:
+ $cs = $csa->fetch_top_level();
+
+ #Get a particular version of a top_level coord system:
+ $cs = $csa->fetch_top_level('NCBI34');
+
+ #Get all top level coord systems:
+ foreach $cs (@{$csa->fetch_all_top_level()}) {
+ print $cs->name(), ' ', $cs->version, "\n";
+ }
+
+ #can also use an alias in fetch_by_name:
+ $cs = $csa->fetch_by_name('toplevel');
+
+ #
+ # Fetching by sequence level:
+ #
+
+ #Get the coord system which is used to store sequence:
+ $cs = $csa->fetch_sequence_level();
+
+ #can also use an alias in fetch_by_name:
+ $cs = $csa->fetch_by_name('seqlevel');
+
+ #
+ # Fetching by id
+ #
+ $cs = $csa->fetch_by_dbID(1);
+
+
+=head1 DESCRIPTION
+
+This adaptor allows the querying of information from the coordinate system
+adaptor.
+
+Note that many coordinate systems do not have a concept of a version
+for the entire coordinate system (though they may have a per-sequence version).
+The 'chromosome' coordinate system usually has a version (i.e. the
+assembly version) but the clonal coordinate system does not (despite having
+individual sequence versions). In the case where a coordinate system does
+not have a version an empty string ('') is used instead.
+
+=head1 AUTHOR - Graham McVicker
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL development list ensembl-dev@ebi.ac.uk
+
+=head1 APPENDIX
+
+The rest of the documentation details each of the object
+methods. Internal methods are usually preceded with a _
+
+=cut
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::DBSQL::CoordSystemAdaptor;
+
+use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
+use Bio::EnsEMBL::CoordSystem;
+use DBI;
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+
+
+=head2 new
+
+ Arg [1] : See BaseAdaptor for arguments (none specific to this
+ subclass)
+ Example : $cs = $db->get_CoordSystemAdaptor(); #better than new()
+ Description: Creates a new CoordSystem adaptor and caches the contents
+ of the coord_system table in memory.
+ Returntype : Bio::EnsEMBL::DBSQL::CoordSystemAdaptor
+ Exceptions : none
+ Caller :
+
+=cut
+
+sub new {
+ my $caller = shift;
+
+ my $class = ref($caller) || $caller;
+
+ my $self = $class->SUPER::new(@_);
+
+ #
+ # Cache the entire contents of the coord_system table cross-referenced
+ # by dbID and name
+ #
+
+ #keyed on name, list of coord_system value
+ $self->{'_name_cache'} = {};
+
+ #keyed on id, coord_system value
+ $self->{'_dbID_cache'} = {};
+
+ #keyed on id, 1/undef values
+ $self->{'_is_sequence_level'} = {};
+ $self->{'_is_top_level'} = {};
+ $self->{'_is_default_version'} = {};
+
+ my $sth = $self->prepare('SELECT coord_system_id, name, version, attrib ' .
+ 'FROM coord_system');
+
+ $sth->execute();
+
+ my ($dbID, $name, $version, $attrib);
+ $sth->bind_columns(\$dbID, \$name, \$version, \$attrib);
+
+ while($sth->fetch()) {
+ my $seq_lvl = 0;
+ my $top_lvl = 0;
+ my $default = 0;
+ if($attrib) {
+ foreach my $attrib (split(',', $attrib)) {
+ $self->{"_is_$attrib"}->{$dbID} = 1;
+ if($attrib eq 'sequence_level') {
+ $seq_lvl = 1;
+ } elsif($attrib eq 'top_level') {
+ $top_lvl = 1;
+ } elsif($attrib eq 'default_version') {
+ $default = 1;
+ }
+ }
+ }
+
+ my $cs = Bio::EnsEMBL::CoordSystem->new
+ (-DBID => $dbID,
+ -ADAPTOR => $self,
+ -NAME => $name,
+ -VERSION => $version,
+ -TOP_LEVEL => $top_lvl,
+ -SEQUENCE_LEVEL => $seq_lvl,
+ -DEFAULT => $default);
+
+ $self->{'_dbID_cache'}->{$dbID} = $cs;
+
+ $self->{'_name_cache'}->{lc($name)} ||= [];
+ push @{$self->{'_name_cache'}->{lc($name)}}, $cs;
+ }
+ $sth->finish();
+
+ #
+ # Retrieve the list of the coordinate systems that features are stored in
+ # and cache them
+ #
+ $sth = $self->prepare('SELECT table_name, coord_system_id FROM meta_coord');
+ $sth->execute();
+ while(my ($table_name, $dbID) = $sth->fetchrow_array()) {
+ $self->{'_feature_cache'}->{lc($table_name)} ||= [];
+ my $cs = $self->{'_dbID_cache'}->{$dbID};
+ if(!$cs) {
+ throw("meta_coord table refers to non-existant coord_system id=[$dbID]");
+ }
+ push @{$self->{'_feature_cache'}->{lc($table_name)}}, $cs;
+ }
+ $sth->finish();
+
+ #
+ # Retrieve a list of available mappings from the meta table.
+ # this may eventually be moved a table of its own if this proves too
+ # cumbersome
+ #
+
+ my %mappings;
+ my $mc = $self->db()->get_MetaContainer();
+ foreach my $map_pair (@{$mc->list_value_by_key('assembly.mapping')}) {
+ my ($asm,$cmp) = split(/\|/, $map_pair);
+ if(!$cmp || !$cmp) {
+ throw('incorrectly formatted assembly.mapping values in meta table');
+ }
+ my($asm_name, $asm_version) = split(/:/, $asm);
+ my($cmp_name, $cmp_version) = split(/:/, $cmp);
+
+ my $cmp_cs = $self->fetch_by_name($cmp_name,$cmp_version);
+ my $asm_cs = $self->fetch_by_name($asm_name,$asm_version);
+
+ $mappings{$asm_cs->dbID} ||= {};
+ $mappings{$asm_cs->dbID}->{$cmp_cs->dbID} = 1;
+ }
+
+ $self->{'_mapping_paths'} = \%mappings;
+
+ return $self;
+}
+
+
+
+=head2 fetch_all
+
+ Arg [1] : none
+ Example : foreach my $cs (@{$csa->fetch_all()}) {
+ print $cs->name(), ' ', $cs->version(), "\n";
+ }
+ Description: Retrieves every coordinate system defined in the DB
+ Returntype : listref of Bio::EnsEMBL::CoordSystems
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub fetch_all {
+ my $self = shift;
+
+ my @coord_systems = values %{$self->{'_dbID_cache'}};
+
+ return \@coord_systems;
+}
+
+
+
+=head2 fetch_by_name
+
+ Arg [1] : string $name
+ The name of the coordinate system to retrieve. Alternatively
+ this may be an alias for a real coordinate system. Valid
+ aliases are 'toplevel' and 'seqlevel'.
+ Arg [2] : string $version (optional)
+ The version of the coordinate system to retrieve. If not
+ specified the default version will be used.
+ Example : $coord_sys = $csa->fetch_by_name('clone');
+ $coord_sys = $csa->fetch_by_name('chromosome', 'NCBI33');
+ #toplevel is an alias for the highest coordinate system
+ #such as the chromosome coordinate system
+ $coord_sys = $csa->fetch_by_name('toplevel');
+ $coord_sys = $csa->fetch_by_name('toplevel', 'NCBI31');
+ #seqlevel is an alias for the sequence level coordinate system
+ #such as the clone or contig coordinate system
+ $coord_sys = $csa->fetch_by_name('seqlevel');
+ Description: Retrieves a coordinate system by its name
+ Returntype : Bio::EnsEMBL::CoordSystem
+ Exceptions : throw if the requested coordinate system does not exist
+ throw if no name argument provided
+ warning if no version provided and default does not exist
+ Caller : general
+
+=cut
+
+sub fetch_by_name {
+ my $self = shift;
+ my $name = lc(shift); #case insensitve matching
+ my $version = shift;
+
+ throw('Name argument is required.') if(!$name);
+
+ $version = lc($version) if($version);
+
+
+ if($name eq 'seqlevel') {
+ return $self->fetch_sequence_level();
+ } elsif($name eq 'toplevel') {
+ return $self->fetch_top_level($version);
+ }
+
+ if(!exists($self->{'_name_cache'}->{$name})) {
+ my $guess = '';
+ if($name =~ /top/) {
+ $guess = "\nDid you mean 'toplevel' instead of '$name'?";
+ } elsif($name =~ /seq/) {
+ $guess = "\nDid you mean 'seqlevel' instead of '$name'?";
+ }
+ throw("Coord_system with name [$name] does not exist.$guess");
+ }
+
+ my @coord_systems = @{$self->{'_name_cache'}->{$name}};
+
+ throw("Coord_system with name [$name] does not exist.") if(!@coord_systems);
+
+ foreach my $cs (@coord_systems) {
+ if($version) {
+ return $cs if(lc($cs->version()) eq $version);
+ } elsif($self->{'_is_default_version'}->{$cs->dbID()}) {
+ return $cs;
+ }
+ }
+
+ if($version) {
+ throw("Coord_system [$name] does not exist with version [$version]");
+ }
+
+ #didn't find a default, just take first one
+ my $cs = shift @coord_systems;
+ my $v = $cs->version();
+ warning("No default version for coord_system [$name] exists. " .
+ "Using version [$v] arbitrarily");
+
+ return $cs;
+}
+
+
+=head2 fetch_all_by_name
+
+ Arg [1] : string $name
+ The name of the coordinate system to retrieve. This can be
+ the name of an actual coordinate system or an alias for a
+ coordinate system. Valid aliases are 'toplevel' and 'seqlevel'.
+ Example : foreach my $cs (@{$csa->fetch_all_by_name('chromosome')}){
+ print $cs->name(), ' ', $cs->version();
+ }
+ Description: Retrieves all coordinate systems of a particular name
+ Returntype : listref of Bio::EnsEMBL::CoordSystem objects
+ Exceptions : throw if no name argument provided
+ Caller : general
+
+=cut
+
+sub fetch_all_by_name {
+ my $self = shift;
+ my $name = lc(shift); #case insensitive matching
+
+ throw('Name argument is required') if(!$name);
+
+ if($name eq 'seqlevel') {
+ return [$self->fetch_sequence_level()];
+ } elsif($name eq 'toplevel') {
+ return $self->fetch_all_top_level();
+ }
+
+ return $self->{'_name_cache'}->{$name} || [];
+}
+
+
+
+
+=head2 fetch_all_by_feature_table
+
+ Arg [1] : string $table - the name of the table to retrieve coord systems
+ for
+ Example : my @coord_systems = $csa->fetch_by_feature_table('gene')
+ Description: This retrieves the list of coordinate systems that features
+ in a particular table are stored. It is used internally by
+ the API to perform queries to these tables and to ensure that
+ features are only stored in appropriate coordinate systems.
+ Returntype : listref of Bio::EnsEMBL::CoordSystem objects
+ Exceptions : none
+ Caller : BaseFeatureAdaptor
+
+=cut
+
+sub fetch_all_by_feature_table {
+ my $self = shift;
+ my $table = lc(shift); #case insensitive matching
+
+ throw('Name argument is required') unless $table;
+
+ my $result = $self->{'_feature_cache'}->{$table};
+
+ if(!$result) {
+ throw("Feature table [$table] does not have a defined coordinate system" .
+ " in the meta_coord table");
+ }
+
+ return $result;
+}
+
+
+=head2 add_feature_table
+
+ Arg [1] : Bio::EnsEMBL::CoordSystem $cs
+ The coordinate system to associate with a feature table
+ Arg [2] : string $table - the name of the table in which features of
+ a given coordinate system will be stored in
+ Example : $csa->add_feature_table($chr_coord_system, 'gene');
+ Description: This function tells the coordinate system adaptor that
+ features from a specified table will be stored in a certain
+ coordinate system. If this information is not already stored
+ in the database it will be added.
+ Returntype : none
+ Exceptions : none
+ Caller : BaseFeatureAdaptor
+
+=cut
+
+
+sub add_feature_table {
+ my $self = shift;
+ my $cs = shift;
+ my $table = lc(shift);
+
+ if(!ref($cs) || !$cs->isa('Bio::EnsEMBL::CoordSystem')) {
+ throw('CoordSystem argument is required.');
+ }
+
+ if(!$table) {
+ throw('Table argument is required.');
+ }
+
+ my $coord_systems = $self->{'_feature_cache'}->{$table};
+
+ my ($exists) = grep {$_->equals($cs)} @$coord_systems;
+
+ #do not do anything if this feature table is already associated with the
+ #coord system
+ return if($exists);
+
+ #make sure to use a coord system stored in this database so that we
+ #do not use the wrong coord_system_id
+ if(!$cs->is_stored($self->db())) {
+ $cs = $self->fetch_by_name($cs->name(), $cs->version);
+ throw("CoordSystem not found in database.");
+ }
+
+ #store the new tablename -> coord system relationship in the db
+ #ignore failures b/c during the pipeline multiple processes may try
+ #to update this table and only the first will be successful
+ my $sth = $self->prepare('INSERT IGNORE INTO meta_coord ' .
+ 'SET coord_system_id = ?, ' .
+ 'table_name = ?');
+
+ $sth->execute($cs->dbID, $table);
+
+ #update the internal cache
+ $self->{'_feature_cache'}->{$table} ||= [];
+ push @{$self->{'_feature_cache'}->{$table}}, $cs;
+
+ return;
+}
+
+
+
+=head2 fetch_by_dbID
+
+ Arg [1] : int dbID
+ Example : $cs = $csa->fetch_by_dbID(4);
+ Description: Retrieves a coord_system via its internal
+ identifier.
+ Returntype : Bio::EnsEMBL::CoordSystem
+ Exceptions : thrown if no coord_system exists for specified dbID
+ Caller : general
+
+=cut
+
+sub fetch_by_dbID {
+ my $self = shift;
+ my $dbID = shift;
+
+ throw('dbID argument is required') if(!$dbID);
+
+ my $cs = $self->{'_dbID_cache'}->{$dbID};
+
+ throw("Coord_system with dbID [$dbID] does not exist") if(!$cs);
+
+ return $cs;
+}
+
+
+
+=head2 fetch_top_level
+
+ Arg [1] : string $version (optional)
+ The version of the top-level to obtain. If not provided
+ the default version top-level will be returned.
+ Example : $cs = $csa->fetch_top_level();
+ $cs = $csa->fetch_top_level('NCBI34');
+ Description: Retrieves the top level coordinate system. It is possible
+ to have multiple top-level coordinate systems if there is
+ more than one version of the top-level system. For
+ example the top level of a homo_sapiens database could
+ be 'chromosome' but this might have 'NCBI33', 'NCBI31',
+ and 'NCBI34' versions. One of these will be defined as
+ the default and will be returned if no specific version
+ is requested. If a specific version is requested then it
+ will be returned.
+ Returntype : a Bio::EnsEMBL::CoordSystem object
+ Exceptions : throw if no top-level coord_system exists for specified
+ version
+ throw if no top-level coord_system exists at all
+ warning if no version specified and no default version
+ exists
+ Caller : general
+
+=cut
+
+sub fetch_top_level {
+ my $self = shift;
+
+ return $self->_fetch_by_attrib('top_level', @_);
+}
+
+
+=head2 fetch_all_top_level
+
+ Arg [1] : none
+ Example : foreach my $cs (@{$csa->fetch_all_top_level()}) {
+ my ($id, $name, $version) = @$cs;
+ }
+ Description: Retrieves all top level coordinate systems defined in
+ the DB. It is possible to have multiple top-level
+ coordinate systems if there is more than one version
+ of the top-level system. For example the top level of a
+ homo_sapiens database could be 'chromosome' but this
+ might have 'NCBI33', 'NCBI31', and 'NCBI34' versions.
+ Returntype : listref of Bio::EnsEMBL::CoordSystem objects
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub fetch_all_top_level {
+ my $self = shift;
+
+ return $self->_fetch_all_by_attrib('top_level', @_);
+}
+
+
+
+=head2 fetch_sequence_level
+
+ Arg [1] : none
+ Example : ($id, $name, $version) = $csa->fetch_sequence_level();
+ Description: Retrieves the coordinate system at which sequence
+ is stored at.
+ Returntype : Bio::EnsEMBL::CoordSystem
+ Exceptions : throw if no sequence_level coord system exists at all
+ throw if multiple sequence_level coord systems exists
+ Caller : general
+
+=cut
+
+sub fetch_sequence_level {
+ my $self = shift;
+
+ my @dbIDs = keys %{$self->{'_is_sequence_level'}};
+
+ throw('No sequence_level coord_system is defined') if(!@dbIDs);
+
+ if(@dbIDs > 1) {
+ throw('Multiple sequence_level coord_systems are defined.' .
+ 'Only one is currently supported');
+ }
+
+ return $self->{'_dbID_cache'}->{$dbIDs[0]};
+}
+
+
+
+
+=head2 get_mapping_path
+
+ Arg [1] : Bio::EnsEMBL::CoordSystem $cs1
+ Arg [2] : Bio::EnsEMBL::CoordSystem $cs2
+ Example : foreach my $cs @{$csa->get_mapping_path($cs1,$cs2);
+ Description: Given two coordinate systems this will return a mapping path
+ between them. The path is formatted as a list of coordinate
+ systems starting with the assembled coord systems and
+ descending through component systems. For example, if the
+ following mappings were defined in the meta table:
+ chromosome -> clone
+ clone -> contig
+
+ And the contig and chromosome coordinate systems where
+ provided as arguments like so:
+ $csa->get_mapping_path($chr_cs,$ctg_cs);
+
+ The return values would be:
+ [$chr_cs, $clone_cs, $contig_cs]
+
+ The return value would be the same even if the order of
+ arguments was reversed.
+
+ This becomes a bit more problematic when the relationship is
+ something like:
+ chromosome -> contig
+ clone -> contig
+
+ In this case the contig coordinate system is the component
+ coordinate system for both mappings and for the following
+ request:
+ $csa->get_mappging_path($chr_cs, $cln_cs);
+
+ Either of the following mapping paths would be valid:
+ [$chr_cs, $contig_cs, $clone_cs]
+ or
+ [$clone_cs, $contig_cs, $chr_cs]
+
+ Also note that the ordering of the above is not
+ assembled to component but rather
+ assembled -> component -> assembled.
+
+ If no mapping path exists, an reference to an empty list is
+ returned.
+
+ Returntype : listref of coord_sytem ids ordered from assembled to smaller
+ component coord_systems
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub get_mapping_path {
+ my $self = shift;
+ my $cs1 = shift;
+ my $cs2 = shift;
+ my $seen = shift || {};
+
+ $self->{'_shortest_path'} ||= {};
+
+ my $cs1_id = $cs1->dbID();
+ my $cs2_id = $cs2->dbID();
+
+ # if this method has already been called with the same arguments
+ # return the cached result
+ if($self->{'_shortest_path'}->{"$cs1_id:$cs2_id"}) {
+ return $self->{'_shortest_path'}->{"$cs1_id:$cs2_id"};
+ }
+
+ #if we have already seen this pair then there is some circular logic
+ #encoded in the mappings. This is not good.
+ if($seen->{"$cs1_id:$cs2_id"}) {
+ throw("Circular logic detected in defined assembly mappings");
+ }
+
+ #if there is a direct mapping between this coord system and other one
+ #then path between cannot be shorter, just return the one step path
+ if($self->{'_mapping_paths'}->{$cs1_id}->{$cs2_id}) {
+ $self->{'_shortest_path'}->{"$cs1_id:$cs2_id"} = [$cs1,$cs2];
+ $self->{'_shortest_path'}->{"$cs2_id:$cs1_id"} = [$cs1,$cs2];
+ return [$cs1,$cs2];
+ }
+ if($self->{'_mapping_paths'}->{$cs2_id}->{$cs1_id}) {
+ $self->{'_shortest_path'}->{"$cs1_id:$cs2_id"} = [$cs2,$cs1];
+ $self->{'_shortest_path'}->{"$cs2_id:$cs1_id"} = [$cs2,$cs1];
+ return [$cs2,$cs1];
+ }
+
+ $seen->{"$cs1_id:$cs2_id"} = 1;
+ $seen->{"$cs2_id:$cs1_id"} = 1;
+
+ # There is no direct mapping available, but there may be an indirect
+ # path. Call this method recursively on the components of both paths.
+ my $shortest;
+
+ #need to try both as assembled since we do not know which is the assembled
+ #coord_system and which is the component
+ foreach my $pair ([$cs1,$cs2], [$cs2,$cs1]) {
+ my ($asm_cs, $target_cs) = @$pair;
+ my $asm_cs_id = $asm_cs->dbID();
+
+ foreach my $cmp_cs_id (keys %{$self->{'_mapping_paths'}->{$asm_cs_id}}) {
+ my $cmp_cs = $self->fetch_by_dbID($cmp_cs_id);
+ my $path = $self->get_mapping_path($cmp_cs, $target_cs, $seen);
+ my $len = @$path;
+ my $shortest;
+
+ next if($len == 0);
+
+ #Check whether the component was used as an assembled
+ #or component in the next part of the path:
+ if($cmp_cs_id == $path->[0]->dbID) {
+ $path = [$asm_cs, @$path];
+ } else {
+ $path = [@$path, $asm_cs];
+ }
+
+ #shortest possible indirect, add to path so far and return
+ if($len == 2) {
+ $self->{'_shortest_path'}->{"$cs1_id:$cs2_id"} = $path;
+ $self->{'_shortest_path'}->{"$cs2_id:$cs1_id"} = $path;
+ return $path;
+ } elsif(!defined($shortest) || $len+1 < @$shortest) {
+ #keep track of the shortest path found so far,
+ #there may yet be shorter..
+ $shortest = $path;
+ }
+ }
+ #use the shortest path found so far,
+ #if no path was found continue, using the the other id as assembled
+ if(defined($shortest)) {
+ $self->{'_shortest_path'}->{"$cs1_id:$cs2_id"} = $shortest;
+ $self->{'_shortest_path'}->{"$cs2_id:$cs1_id"} = $shortest;
+ return $shortest;
+ }
+ }
+
+ #did not find any possible path
+ $self->{'_shortest_path'}->{"$cs1_id:$cs2_id"} = [];
+ $self->{'_shortest_path'}->{"$cs2_id:$cs1_id"} = [];
+ return [];
+}
+
+
+
+sub _fetch_by_attrib {
+ my $self = shift;
+ my $attrib = shift;
+ my $version = shift;
+
+ $version = lc($version) if($version);
+
+ my @dbIDs = keys %{$self->{"_is_$attrib"}};
+
+ throw("No $attrib coordinate system defined") if(!@dbIDs);
+
+ foreach my $dbID (@dbIDs) {
+ my $cs = $self->{'_dbID_cache'}->{$dbID};
+ if($version) {
+ return $cs if(lc($version) eq $cs->version());
+ } elsif($self->{'_is_default_version'}->{$dbID}) {
+ return $cs;
+ }
+ }
+
+ #specifically requested attrib system was not found
+ if($version) {
+ throw("$attrib coord_system with version [$version] does not exist");
+ }
+
+ #coordsystem with attrib exists but no default is defined:
+ my $dbID = shift @dbIDs;
+ my $cs = $self->{'_dbID_cache'}->{$dbID};
+ my $v = $cs->version();
+ warning("No default version for $attrib coord_system exists. " .
+ "Using version [$v] arbitrarily");
+
+ return $cs;
+}
+
+
+sub _fetch_all_by_attrib {
+ my $self = shift;
+ my $attrib = shift;
+
+ my @coord_systems = ();
+ foreach my $dbID (keys %{$self->{"_is_$attrib"}}) {
+ push @coord_systems, $self->{"_dbID_cache"}->{$dbID};
+ }
+
+ return \@coord_systems;
+}
+
+
+#
+# Called during db destruction to clean up internal cache structures etc.
+#
+sub deleteObj {
+ my $self = shift;
+
+ #break circular adaptor <-> db references
+ $self->SUPER::deleteObj();
+
+ #breack circular object <-> adaptor references
+ delete $self->{'_feature_cache'};
+ delete $self->{'_name_cache'};
+ delete $self->{'_dbID_cache'};
+ delete $self->{'_mapping_paths'};
+ delete $self->{'_shortest_paths'};
+}
+
+
+=head2 store
+
+ Arg [1] : Bio::EnsEMBL::CoordSystem
+ Example : $csa->store($coord_system);
+ Description: Stores a CoordSystem object in the database.
+ Returntype : none
+ Exceptions : Warning if CoordSystem is already stored in this database.
+ Caller : none
+
+=cut
+
+sub store {
+ my $self = shift;
+ my $cs = shift;
+
+ if(!$cs || !ref($cs) || !$cs->isa('Bio::EnsEMBL::CoordSystem')) {
+ throw('CoordSystem argument expected.');
+ }
+
+ my $db = $self->db();
+ my $name = $cs->name();
+ my $version = $cs->version();
+ my $toplevel = $cs->is_top_level();
+ my $seqlevel = $cs->is_sequence_level();
+ my $default = $cs->is_default();
+
+ #
+ # Do lots of sanity checking to prevent bad data from being entered
+ #
+
+ if($cs->is_stored($db)) {
+ warning("CoordSystem $name $version is already in db.\n");
+ return;
+ }
+
+ if($name eq 'toplevel' || $name eq 'seqlevel' || !$name) {
+ throw("[$name] is not a valid name for a CoordSystem.");
+ }
+
+ if($seqlevel && keys(%{$self->{'_is_sequence_level'}})) {
+ throw("There can only be one sequence level CoordSystem.");
+ }
+
+ if(exists $self->{'_name_cache'}->{lc($name)}) {
+ my @coord_systems = @{$self->{'_name_cache'}->{lc($name)}};
+ foreach my $c (@coord_systems) {
+ if(lc($c->version()) eq lc($version)) {
+ warning("CoordSystem $name $version is already in db.\n");
+ return;
+ }
+ if($default && $self->{'_is_default_version'}->{$c->dbID()}) {
+ throw("There can only be one default version of CoordSystem $name");
+ }
+ }
+ }
+
+ my @attrib;
+
+ push @attrib, 'top_level' if($toplevel);
+ push @attrib, 'default_version' if($default);
+ push @attrib, 'sequence_level' if($seqlevel);
+
+ my $attrib_str = (@attrib) ? join(',', @attrib) : undef;
+
+ #
+ # store the coordinate system in the database
+ #
+
+ my $sth = $db->prepare('INSERT INTO coord_system ' .
+ 'SET name = ?, ' .
+ 'version = ?, ' .
+ 'attrib = ?');
+
+ $sth->execute($name, $version, $attrib_str);
+ my $dbID = $sth->{'mysql_insertid'};
+ $sth->finish();
+
+ if(!$dbID) {
+ throw("Did not get dbID from store of CoordSystem.");
+ }
+
+ $cs->dbID($dbID);
+ $cs->adaptor($self);
+
+ #
+ # update the internal caches that are used for fetching
+ #
+ $self->{'_is_default_version'}->{$dbID} = 1 if($default);
+ $self->{'_is_sequence_level'}->{$dbID} = 1 if($seqlevel);
+ $self->{'_is_top_level'}->{$dbID} = 1 if($toplevel);
+
+ $self->{'_name_cache'}->{lc($name)} ||= [];
+ push @{$self->{'_name_cache'}->{lc($name)}}, $cs;
+
+ $self->{'_dbID_cache'}->{$dbID} = $cs;
+
+ return $cs;
+}
+
+
+
+
+1;
+
+
+
+
diff --git a/modules/Bio/EnsEMBL/DBSQL/DBAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/DBAdaptor.pm
index 357341a58e..e97da6b59c 100755
--- a/modules/Bio/EnsEMBL/DBSQL/DBAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/DBAdaptor.pm
@@ -16,27 +16,28 @@
$slice = $db->get_SliceAdaptor()->fetch_by_chr_start_end('X', 1, 10000);
-
=head1 DESCRIPTION
-This object represents a database that is implemented somehow (you shouldn\'t
-care much as long as you can get the object). Once created you can retrieve
-database adaptors specific to various database objects that allow the
-retrieval and creation of objects from the database,
+This is the primary interface to an EnsEMBL database. It maintains an active
+connection to the database and allows for the retrieval of ObjectAdaptors,
+via a set of get_XxxxAdaptor methods (where Xxxx is the type of adaptor).
+
+ObjectAdaptors can then be used to obtain objects and actual information
+from the database.
+
=head1 CONTACT
Post questions to the EnsEMBL development list <ensembl-dev@ebi.ac.uk>
-=head1 APPENDIX
+=head1 METHODS
-The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
+The rest of the documentation details each of the object methods. Internal
+methods are usually preceded with a _
=cut
-# Let the code begin...
-
package Bio::EnsEMBL::DBSQL::DBAdaptor;
use vars qw(@ISA);
@@ -45,6 +46,9 @@ use strict;
use Bio::EnsEMBL::DBSQL::DBConnection;
use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning deprecate);
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
@ISA = qw(Bio::EnsEMBL::DBSQL::DBConnection);
@@ -56,9 +60,8 @@ use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
Arg [-DNADB]: (optional) Bio::EnsEMBL::DBSQL::DBAdaptor DNADB
All sequence, assembly, contig information etc, will be
- retrieved from this database instead.
- Arg [-TYPE]: (optional) An assembly_type
- Arg [..] : Other args are passed to superclass
+ retrieved from this database instead.
+ Arg [..] : Other args are passed to superclass
Bio::EnsEMBL::DBSQL::DBConnection
Example : $db = new Bio::EnsEMBL::DBSQL::DBAdaptor(
-user => 'root',
@@ -74,57 +77,70 @@ use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
sub new {
my($class, @args) = @_;
-
+
#call superclass constructor
my $self = $class->SUPER::new(@args);
-
- my ( $dnadb, $assembly_type ) = $self->_rearrange([qw(DNADB TYPE)],@args);
+
+ my ( $dnadb ) = rearrange([qw(DNADB)],@args);
if(defined $dnadb) {
$self->dnadb($dnadb);
}
- if ($assembly_type) {
- $self->assembly_type($assembly_type);
- }
# $self here is actually a Container object
# so need to call _obj to get the DBAdaptor
- $self->_obj->{'default_module'} = {
- 'ArchiveStableId' => 'Bio::EnsEMBL::DBSQL::ArchiveStableIdAdaptor',
- 'QtlFeature' => 'Bio::EnsEMBL::Map::DBSQL::QtlFeatureAdaptor',
- 'Qtl' => 'Bio::EnsEMBL::Map::DBSQL::QtlAdaptor',
- 'ProteinFeature' => 'Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor',
- 'Protein' => 'Bio::EnsEMBL::DBSQL::ProteinAdaptor',
- 'MapFrag' => 'Bio::EnsEMBL::DBSQL::MapFragAdaptor',
- 'DnaAlignFeature' => 'Bio::EnsEMBL::DBSQL::DnaAlignFeatureAdaptor',
- 'Clone' => 'Bio::EnsEMBL::DBSQL::CloneAdaptor',
- 'PredictionTranscript' => 'Bio::EnsEMBL::DBSQL::PredictionTranscriptAdaptor',
- 'Sequence' => 'Bio::EnsEMBL::DBSQL::SequenceAdaptor',
- 'Gene' => 'Bio::EnsEMBL::DBSQL::GeneAdaptor',
- 'Exon' => 'Bio::EnsEMBL::DBSQL::ExonAdaptor',
- 'Transcript' => 'Bio::EnsEMBL::DBSQL::TranscriptAdaptor',
- 'Translation' => 'Bio::EnsEMBL::DBSQL::TranslationAdaptor',
- 'RawContig' => 'Bio::EnsEMBL::DBSQL::RawContigAdaptor',
- 'Slice' => 'Bio::EnsEMBL::DBSQL::SliceAdaptor',
- 'Analysis' => 'Bio::EnsEMBL::DBSQL::AnalysisAdaptor',
- 'SimpleFeature' => 'Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor',
- 'RepeatConsensus' => 'Bio::EnsEMBL::DBSQL::RepeatConsensusAdaptor',
- 'ProteinAlignFeature' => 'Bio::EnsEMBL::DBSQL::ProteinAlignFeatureAdaptor',
- 'AssemblyMapper' => 'Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor',
- 'DBEntry' => 'Bio::EnsEMBL::DBSQL::DBEntryAdaptor',
- 'KaryotypeBand' => 'Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor',
- 'Chromosome' => 'Bio::EnsEMBL::DBSQL::ChromosomeAdaptor',
- 'SupportingFeature' => 'Bio::EnsEMBL::DBSQL::SupportingFeatureAdaptor',
- 'MarkerFeature' => 'Bio::EnsEMBL::Map::DBSQL::MarkerFeatureAdaptor',
- 'Marker' => 'Bio::EnsEMBL::Map::DBSQL::MarkerAdaptor',
- 'RepeatFeature' => 'Bio::EnsEMBL::DBSQL::RepeatFeatureAdaptor',
- 'ProxySNP' => 'Bio::EnsEMBL::DBSQL::ProxySNPAdaptor',
- 'ProxyGene' => 'Bio::EnsEMBL::DBSQL::ProxyGeneAdaptor',
- 'ProxyRepeatFeature' => 'Bio::EnsEMBL::DBSQL::ProxyRepeatFeatureAdaptor',
- 'Blast' => 'Bio::EnsEMBL::External::BlastAdaptor' };
+ $self->_obj->{'default_module'} =
+ { 'Analysis' => 'Bio::EnsEMBL::DBSQL::AnalysisAdaptor',
+ 'ArchiveStableId' => 'Bio::EnsEMBL::DBSQL::ArchiveStableIdAdaptor',
+ 'AssemblyExceptionFeature' => 'Bio::EnsEMBL::DBSQL::AssemblyExceptionFeatureAdaptor',
+ 'AssemblyMapper' => 'Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor',
+ 'Blast' => 'Bio::EnsEMBL::External::BlastAdaptor',
+ 'Chromosome' => 'Bio::EnsEMBL::DBSQL::ChromosomeAdaptor',
+ 'Clone' => 'Bio::EnsEMBL::DBSQL::CloneAdaptor',
+ 'CoordSystem' => 'Bio::EnsEMBL::DBSQL::CoordSystemAdaptor',
+ 'CompressedSequence' => 'Bio::EnsEMBL::DBSQL::CompressedSequenceAdaptor',
+ 'DBEntry' => 'Bio::EnsEMBL::DBSQL::DBEntryAdaptor',
+ 'DnaAlignFeature' => 'Bio::EnsEMBL::DBSQL::DnaAlignFeatureAdaptor',
+ 'DensityFeature' => 'Bio::EnsEMBL::DBSQL::DensityFeatureAdaptor',
+ 'Exon' => 'Bio::EnsEMBL::DBSQL::ExonAdaptor',
+ 'Gene' => 'Bio::EnsEMBL::DBSQL::GeneAdaptor',
+ 'KaryotypeBand' => 'Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor',
+ 'MapFrag' => 'Bio::EnsEMBL::DBSQL::MapFragAdaptor',
+ 'Marker' => 'Bio::EnsEMBL::Map::DBSQL::MarkerAdaptor',
+ 'MarkerFeature' =>
+ 'Bio::EnsEMBL::Map::DBSQL::MarkerFeatureAdaptor',
+ 'MetaContainer' => 'Bio::EnsEMBL::DBSQL::MetaContainer',
+ 'MiscSet' => 'Bio::EnsEMBL::DBSQL::MiscSetAdaptor',
+ 'MiscFeature' => 'Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor',
+ 'PredictionTranscript' =>
+ 'Bio::EnsEMBL::DBSQL::PredictionTranscriptAdaptor',
+ 'PredictionExon' => 'Bio::EnsEMBL::DBSQL::PredictionExonAdaptor',
+ 'ProteinFeature' => 'Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor',
+ 'Protein' => 'Bio::EnsEMBL::DBSQL::ProteinAdaptor',
+ 'ProteinAlignFeature' =>
+ 'Bio::EnsEMBL::DBSQL::ProteinAlignFeatureAdaptor',
+ 'ProxySNP' => 'Bio::EnsEMBL::DBSQL::ProxySNPAdaptor',
+ 'ProxyGene' => 'Bio::EnsEMBL::DBSQL::ProxyGeneAdaptor',
+ 'ProxyRepeatFeature' =>
+ 'Bio::EnsEMBL::DBSQL::ProxyRepeatFeatureAdaptor',
+ 'QtlFeature' => 'Bio::EnsEMBL::Map::DBSQL::QtlFeatureAdaptor',
+ 'Qtl' => 'Bio::EnsEMBL::Map::DBSQL::QtlAdaptor',
+ 'RawContig' => 'Bio::EnsEMBL::DBSQL::RawContigAdaptor',
+ 'RepeatConsensus' => 'Bio::EnsEMBL::DBSQL::RepeatConsensusAdaptor',
+ 'RepeatFeature' => 'Bio::EnsEMBL::DBSQL::RepeatFeatureAdaptor',
+ 'Sequence' => 'Bio::EnsEMBL::DBSQL::SequenceAdaptor',
+ 'SimpleFeature' => 'Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor',
+ 'Slice' => 'Bio::EnsEMBL::DBSQL::SliceAdaptor',
+ 'SupportingFeature' =>
+ 'Bio::EnsEMBL::DBSQL::SupportingFeatureAdaptor',
+ 'Transcript' => 'Bio::EnsEMBL::DBSQL::TranscriptAdaptor',
+ 'Translation' => 'Bio::EnsEMBL::DBSQL::TranslationAdaptor',
+
+ };
+
# initialise storage for hash of names of current modules
- %{$self->_obj->{'current_module'}} = %{$self->_obj->{'default_module'}};
+ %{$self->_obj->{'current_module'}} = %{$self->_obj->{'default_module'}};
# keep a hash of objects representing objects of each adaptor type
# instantiated as required in get adaptor
@@ -133,6 +149,8 @@ sub new {
# initialise generic feature adaptor storage
$self->_obj->{'generic_feature_adaptors'} = {};
+
+
return $self;
}
@@ -143,7 +161,7 @@ sub new {
Args : none
Example : none
Description: ...
- Returntype : Bio::EnsEMBL::DBSQL::ArchiveStableIdAdaptor
+ Returntype : Bio::EnsEMBL::DBSQL::ArchiveStableIdAdaptor
Exceptions : none
Caller : general
@@ -151,7 +169,7 @@ sub new {
sub get_ArchiveStableIdAdaptor {
my( $self ) = @_;
-
+
return
$self->get_adaptor("ArchiveStableId");
}
@@ -162,17 +180,15 @@ sub get_ArchiveStableIdAdaptor {
Args : none
Example : none
Description: ...
- Returntype : Bio::EnsEMBL::Map::DBSQL::QtlFeatureAdaptor
+ Returntype : Bio::EnsEMBL::Map::DBSQL::QtlFeatureAdaptor
Exceptions : none
Caller : general
=cut
sub get_QtlFeatureAdaptor {
- my( $self ) = @_;
-
- return
- $self->get_adaptor("QtlFeature");
+ my $self = shift;
+ return $self->get_adaptor("QtlFeature");
}
=head2 get_QtlAdaptor
@@ -180,17 +196,15 @@ sub get_QtlFeatureAdaptor {
Args : none
Example : none
Description: ...
- Returntype : Bio::EnsEMBL::Map::DBSQL::QtlAdaptor
+ Returntype : Bio::EnsEMBL::Map::DBSQL::QtlAdaptor
Exceptions : none
Caller : general
=cut
sub get_QtlAdaptor {
- my( $self ) = @_;
-
- return
- $self->get_adaptor("Qtl");
+ my $self = shift;
+ return $self->get_adaptor("Qtl");
}
=head2 get_MetaContainer
@@ -200,24 +214,16 @@ sub get_QtlAdaptor {
Description: Gets a MetaContainer object for this database
Returntype : Bio::EnsEMBL::DBSQL::MetaContainer
Exceptions : none
- Caller : ?
+ Caller : general
=cut
sub get_MetaContainer {
- my( $self ) = @_;
-
- my( $mc );
- unless ($mc = $self->{'_meta_container'}) {
- require Bio::EnsEMBL::DBSQL::MetaContainer;
- $mc = Bio::EnsEMBL::DBSQL::MetaContainer->new($self);
- $self->{'_meta_container'} = $mc;
- }
- return $mc;
+ my $self = shift;
+ return $self->get_adaptor('MetaContainer');
}
-
=head2 get_ProteinFeatureAdaptor
Args : none
@@ -231,29 +237,8 @@ sub get_MetaContainer {
=cut
sub get_ProteinFeatureAdaptor {
- my( $self ) = @_;
-
- return
- $self->get_adaptor("ProteinFeature");
-}
-
-
-=head2 get_ProteinAdaptor
-
- Args : none
- Example : $pa = $database_adaptor->get_ProteinAdaptor();
- Description: Gets a ProteinAdaptor for this database.
- Formerly named get_Protein_Adaptor()
- Returntype : Bio::EnsEMBL::DBSQL::ProteinAdaptor
- Exceptions : none
- Caller : general
-
-=cut
-
-sub get_ProteinAdaptor {
- my( $self ) = @_;
-
- return $self->get_adaptor("Protein");
+ my $self = shift;
+ return $self->get_adaptor("ProteinFeature");
}
@@ -278,19 +263,19 @@ sub get_SNPAdaptor {
$primary_adaptor = $lite->get_SNPAdaptor();
} else {
my $snp = $self->get_db_adaptor('SNP');
-
+
unless($snp) {
- warn("No lite or SNP database, cannot get snp adaptor\n");
+ warning("No lite or SNP database, cannot get snp adaptor\n");
return undef;
}
$primary_adaptor = $snp->get_SNPAdaptor();
$primary_adaptor->ensembl_db( $self );
}
-
+
#return a proxy adaptor which can use the lite or the core database
return $self->get_adaptor("ProxySNP",
- $primary_adaptor);
+ $primary_adaptor);
}
@@ -310,105 +295,102 @@ sub get_BlastAdaptor {
my $db_apt = $self->get_db_adaptor('blast');
- return $self->get_adaptor("Blast", $db_apt);
+ return $self->get_adaptor("Blast",
+ $db_apt);
}
-=head2 get_GlovarAdaptor
+=head2 get_PredictionTranscriptAdaptor
Args : none
- Example : $gv_adaptor = $db_adaptor->get_GlovarAdaptor();
- Description: Gets a GlovarAdaptor for retrieving variations
- Returntype : Bio::EnsEMBL::External::Glovar::GlovarAdaptor
+ Example : $pta = $db_adaptor->get_PredictionTranscriptAdaptor();
+ Description: Gets a PredictionTranscriptAdaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::PredictionTranscriptAdaptor
Exceptions : none
Caller : general
=cut
-sub get_GlovarAdaptor {
- my ($self) = @_;
- print "get_db_adaptor in DBAdaptor...\n";
- my $gv_apt = $self->get_db_adaptor('glovar');
- print "get_adaptor in DBAdaptor...\n";
- return $self->get_adaptor("Glovar", $gv_apt);
+sub get_PredictionTranscriptAdaptor {
+ my ($self) = @_;
+ return $self->get_adaptor("PredictionTranscript");
}
-=head2 get_MapFragAdaptor
+=head2 get_PredictionExonAdaptor
- Args : none
- Example : $map_frag_adaptor = $db_adaptor->get_MapFragAdaptor();
- Description: Gets a MapFragAdaptor for this database
- Returntype : Bio::EnsEMBL::DBSQL::MapFragAdaptor
+ Args : none
+ Example : $pea = $db_adaptor->get_PredictionExonAdaptor();
+ Description: Gets a PredictionExonAdaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::PredictionExonAdaptor
Exceptions : none
Caller : general
=cut
-sub get_MapFragAdaptor {
- my $self = shift;
+sub get_PredictionExonAdaptor {
+ my ($self) = @_;
- return $self->get_adaptor( "MapFrag" );
+ return $self->get_adaptor("PredictionExon");
}
-=head2 get_CloneAdaptor
+=head2 get_SequenceAdaptor
Args : none
- Example : $clone_adaptor = $db_adaptor->get_CloneAdaptor();
- Description: Gets a CloneAdaptor for this database
- Returntype : Bio::EnsEMBL::DBSQL::CloneAdaptor
+ Example : $sequence_adaptor = $db_adaptor->get_SequenceAdaptor();
+ Description: Gets a SequenceAdaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::SequenceAdaptor
Exceptions : none
Caller : general
=cut
-sub get_CloneAdaptor {
- my( $self ) = @_;
-
- return $self->dnadb->get_adaptor("Clone");
-}
-
-
-=head2 get_PredictionTranscriptAdaptor
-
- Args : none
- Example : $pta = $db_adaptor->get_PredictionTranscriptAdaptor();
- Description: Gets a PredictionTranscriptAdaptor for this database
- Returntype : Bio::EnsEMBL::DBSQL::PredictionTranscriptAdaptor
- Exceptions : none
- Caller : general
+sub get_SequenceAdaptor {
+ my $self = shift;
-=cut
+ my $mc = $self->dnadb->get_MetaContainer();
-sub get_PredictionTranscriptAdaptor {
- my ($self) = @_;
+ my ($use_compressed) = @{$mc->list_value_by_key('sequence.compression')};
- return
- $self->get_adaptor("PredictionTranscript");
- }
+ if($use_compressed) {
+ return $self->dnadb->get_adaptor("CompressedSequence");
+ } else {
+ #return the sequence adaptor for the dnadb (which may be this db)
+ return $self->dnadb->get_adaptor("Sequence");
+ }
+}
-=head2 get_SequenceAdaptor
+=head2 get_CompressedSequenceAdaptor
Args : none
- Example : $sequence_adaptor = $db_adaptor->get_SequenceAdaptor();
- Description: Gets a SequenceAdaptor for this database
+ Example : $seq_adaptor = $db_adaptor->get_CompressedSequenceAdaptor();
+ Description: Gets a CompressedSequenceAdaptor for this database.
+ A compressed sequence adaptor behaves like a normal
+ SequenceAdaptor except that it stores and retrieves compressed
+ sequence from the dnac table (rather than the dna table).
+ Normally if you want to use compressed sequence you should
+ convert your database to a compressed database with a script
+ and then set the 'sequence.compression' meta_key to a value
+ of '1' in the meta table. This way the compressed sequence
+ adaptor will be used instead of the normal sequence adaptor.
Returntype : Bio::EnsEMBL::DBSQL::SequenceAdaptor
Exceptions : none
Caller : general
=cut
-sub get_SequenceAdaptor {
+sub get_CompressedSequenceAdaptor {
my $self = shift;
#return the sequence adaptor for the dnadb (which may be this db)
- return $self->dnadb->get_adaptor("Sequence");
+ return $self->dnadb->get_adaptor("CompressedSequence");
}
+
=head2 get_GeneAdaptor
Args : none
@@ -486,23 +468,6 @@ sub get_TranslationAdaptor {
}
-=head2 get_RawContigAdaptor
-
- Args : none
- Example : $rca = $db_adaptor->get_RawContigAdaptor();
- Description: Gets a RawContigAdaptor for this database
- Returntype : Bio::EnsEMBL::DBSQL::RawContigAdaptor
- Exceptions : none
- Caller : general
-
-=cut
-
-sub get_RawContigAdaptor {
- my( $self ) = @_;
-
- return $self->dnadb->get_adaptor("RawContig");
-}
-
=head2 get_SliceAdaptor
@@ -518,7 +483,7 @@ sub get_RawContigAdaptor {
sub get_SliceAdaptor {
my( $self ) = @_;
- return $self->get_adaptor("Slice");
+ return $self->dnadb()->get_adaptor("Slice");
}
@@ -631,6 +596,7 @@ sub get_ProteinAlignFeatureAdaptor {
sub get_DnaAlignFeatureAdaptor {
my $self = shift;
+
return $self->get_adaptor("DnaAlignFeature");
}
@@ -656,7 +622,7 @@ sub get_AssemblyMapperAdaptor {
=head2 get_DBEntryAdaptor
- Args : none
+ Args : none
Example : $dbentry_adaptor = $db_adaptor->get_DBEntryAdaptor();
Description: Gets a DBEntryAdaptor for this database
Returntype : Bio::EnsEMBL::DBSQL::DBEntryAdaptor
@@ -673,41 +639,41 @@ sub get_DBEntryAdaptor {
-=head2 get_KaryotypeBandAdaptor
- Args : none
- Example : $kba = $db_adaptor->get_KaryotypeBandAdaptor();
- Description: Gets a KaryotypeBandAdaptor for this database
- Returntype : Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor
+=head2 get_DensityFeatureAdaptor
+
+ Arg [1] : none
+ Example : $density_feature_adaptor = $db_adaptor->get_DBEntryAdaptor();
+ Description: Gets a DensityFeatureAdaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::DensityFeatureAdaptor
Exceptions : none
Caller : general
=cut
-sub get_KaryotypeBandAdaptor {
- my( $self ) = @_;
-
- return
- $self->dnadb->get_adaptor("KaryotypeBand");
+sub get_DensityFeatureAdaptor {
+ my $self = shift;
+ return $self->get_adaptor('DensityFeature');
}
-=head2 get_ChromosomeAdaptor
+
+=head2 get_KaryotypeBandAdaptor
Args : none
- Example : $ca = $db_adaptor->get_ChromosomeAdaptor();
- Description: Gets a ChromosomeAdaptor for this database
- Returntype : Bio::EnsEMBL::DBSQL::ChromosomeAdaptor
+ Example : $kba = $db_adaptor->get_KaryotypeBandAdaptor();
+ Description: Gets a KaryotypeBandAdaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor
Exceptions : none
Caller : general
=cut
-sub get_ChromosomeAdaptor {
+sub get_KaryotypeBandAdaptor {
my( $self ) = @_;
return
- $self->dnadb->get_adaptor("Chromosome");
+ $self->dnadb->get_adaptor("KaryotypeBand");
}
@@ -767,151 +733,82 @@ sub get_MarkerAdaptor {
}
-=head2 list_supported_assemblies
- Args : none
- Example : my @supported = $database_adaptor->list_supported_assemblies);
- Description: Returns a list of assemblies supported by this database.
- Returntype : list of strings
- Exceptions : thrown if SQL query fails
- Caller : ?
+=head2 get_CoordSystemAdaptor
+
+ Arg [1] : none
+ Example : $csa = $db_adaptor->get_CoordSystemAdaptor();
+ Description: Gets a CoordSystemAdaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::CoordSystemAdaptor
+ Exceptions : none
+ Caller : general
=cut
-sub list_supported_assemblies {
- my($self) = @_;
- my @out;
+sub get_CoordSystemAdaptor {
+ my $self = shift;
- my $query = q{
- SELECT distinct type
- FROM assembly
- };
+ return $self->dnadb()->get_adaptor('CoordSystem');
+}
- my $sth = $self->prepare($query) ||
- $self->throw("Error in list_supported_assemblies");
- my $res = $sth->execute ||
- $self->throw("Error in list_supported_assemblies");
- while (my($type) = $sth->fetchrow_array) {
- push(@out, $type);
- }
- return @out;
-}
-=head2 assembly_type
+=head2 get_MiscSetAdaptor
- Arg [1] : (optional) string $value
- the new assembly type value
- Example : $db_adaptor->assembly_type($newval);
- Description: Getter / Setter for the type of assembly used by
- this database. If the value is not set then the
- default value is obtained from the MetaContainer
- Returntype : string
- Exceptions : thrown if there is no defined assembly type, and the default
- assembly type cannot be obtained from the MetaContainer
- Caller : ?
+ Arg [1] : none
+ Example : $msa = $db_adaptor->get_MiscSetAdaptor();
+ Description: Gets a MiscSet adaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::MiscSetAdaptor
+ Exceptions : none
+ Caller : general
=cut
-sub assembly_type{
- my ($obj,$value) = @_;
- if($value) {
- $obj->{'assembly'} = $value;
- }
- if (! defined $obj->{'assembly'}) {
- my $ass;
- eval {
- $ass = $obj->dnadb->get_MetaContainer()->get_default_assembly();
- };
- if ( $@ ) {
- $obj->throw("*** get_MetaContainer->get_default_assembly failed:\n$@\n"
- ."assembly type must be set with assembly_type() first");
- } elsif (! $ass) {
- $obj->throw("No default assembly defined in ".$obj->dnadb->dbname. " - must set with assembly_type() first");
- }
- $obj->{'assembly'} = $ass;
- }
- return $obj->{'assembly'};
+sub get_MiscSetAdaptor {
+ my $self = shift;
+ return $self->get_adaptor('MiscSet');
}
-=head2 assembly_name
- Arg [1] : (optional) string $value
- the new assembly name value
- Example : $db_adaptor->assembly_name($newval);
- Description: Getter / Setter for the name of assembly used by
- this database. If the value is not set then the
- default value is obtained from the MetaContainer
- Returntype : string
- Exceptions : warn if there is no defined assembly name, and the default
- assembly name cannot be obtained from the MetaContainer
- Caller : ?
+=head2 get_MiscFeatureAdaptor
+
+ Arg [1] : none
+ Example : $mfa = $db_adaptor->get_MiscFeatureAdaptor();
+ Description: Gets a MiscFeature adaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor
+ Exceptions : none
+ Caller : general
=cut
-sub assembly_name {
- my ($obj,$value) = @_;
- if($value) {
- $obj->{'assembly_name'} = $value;
- }
- if (! defined $obj->{'assembly_name'}) {
- my $ass;
- eval {
- $ass = $obj->dnadb->get_MetaContainer()->get_default_assembly_name();
- };
- if ( $@ ) {
- warn("*** get_MetaContainer->get_default_assembly_name failed ***\n$@\n");
- } elsif (! $ass) {
- warn("No default assembly name defined in ".$obj->dnadb->dbname. "!");
- }
-
- $obj->{'assembly_name'} = $ass;
- }
- return $obj->{'assembly_name'};
+sub get_MiscFeatureAdaptor {
+ my $self = shift;
+ return $self->get_adaptor('MiscFeature');
}
-=head2 assembly_version
+=head2 get_AssemblyExceptionFeatureAdaptor
- Arg [1] : (optional) string $value
- the new assembly version value
- Example : $db_adaptor->assembly_version($newval);
- Description: Getter / Setter for the version of assembly used by
- this database. If the value is not set then the
- default value is obtained from the MetaContainer
- Returntype : string
- Exceptions : warn if there is no defined assembly version, and the default
- assembly version cannot be obtained from the MetaContainer
- Caller : ?
+ Arg [1] : none
+ Example : $aefa = $db_adaptor->get_AssebmyExceptionFeatureAdaptor();
+ Description: Gets a AssemblyExceptionFeature adaptor for this database
+ Returntype : Bio::EnsEMBL::DBSQL::AssemblyExceptionFeatureAdaptor
+ Exceptions : none
+ Caller : general
=cut
-sub assembly_version {
- my ($obj,$value) = @_;
- if($value) {
- $obj->{'assembly_version'} = $value;
- }
- if (! defined $obj->{'assembly_version'}) {
- my $ass;
- eval {
- $ass = $obj->dnadb->get_MetaContainer()->get_default_assembly_version();
- };
- if ( $@ ) {
- warn("*** get_MetaContainer->get_default_assembly_version failed ***\n$@\n");
- } elsif (! $ass) {
- warn("No default assembly version defined in ".$obj->dnadb->dbname. "!");
- }
-
- $obj->{'assembly_version'} = $ass;
- }
- return $obj->{'assembly_version'};
+sub get_AssemblyExceptionFeatureAdaptor {
+ my $self = shift;
+ return $self->get_adaptor('AssemblyExceptionFeature');
}
+
=head2 dnadb
Title : dnadb
@@ -957,11 +854,6 @@ sub dnadb {
sub deleteObj {
my $self = shift;
-
- if ($self->isa('Bio::EnsEMBL::Container')) {
- $self = $self->_obj;
- }
-
#print "called deleteObj on DBAdaptor\n";
#clean up external feature adaptor references
@@ -975,14 +867,28 @@ sub deleteObj {
foreach my $adaptor_name (keys %{$self->{'current_objects'}}) {
my $adaptor = $self->{'current_objects'}->{$adaptor_name};
- if($adaptor && $adaptor->can('deleteObj')) {
+ if(ref($adaptor) && $adaptor->can('deleteObj')) {
$adaptor->deleteObj();
}
- #print STDERR "DELETING '$adaptor_name'\n";
+
delete $self->{'current_objects'}->{$adaptor_name};
}
}
+ if(exists $self->{'generic_feature_adaptors'}) {
+ foreach my $name (keys %{$self->{'generic_feature_adaptors'}}) {
+ my $adaptor = $self->{'generic_feature_adaptors'}->{$name};
+ if(ref($adaptor) && $adaptor->can('deleteObj')) {
+ $adaptor->deleteObj();
+ }
+
+ delete $self->{'generic_feature_adaptors'}->{$name};
+ }
+
+ delete $self->{'generic_feature_adaptors'};
+ }
+
+
delete $self->{'_meta_container'};
delete $self->{'dnadb'};
@@ -1081,15 +987,15 @@ sub add_ExternalFeatureAdaptor {
unless($adaptor && ref $adaptor &&
$adaptor->isa('Bio::EnsEMBL::External::ExternalFeatureAdaptor')) {
- $self->throw("[$adaptor] is not a " .
- "Bio::EnsEMBL::External::ExternalFeatureAdaptor");
+ throw("[$adaptor] is not a " .
+ "Bio::EnsEMBL::External::ExternalFeatureAdaptor");
}
unless(exists $self->{'_xf_adaptors'}) {
$self->{'_xf_adaptors'} = {};
}
- my $track_name = $adaptor->track_name();
+ my $track_name = $adaptor->{'_track_name'};
#use a generic track name if one hasn't been defined
unless(defined $track_name) {
@@ -1105,6 +1011,7 @@ sub add_ExternalFeatureAdaptor {
$self->{'_xf_adaptors'}->{"$track_name"} = $adaptor;
}
+ $adaptor->db($self);
}
@@ -1171,22 +1078,23 @@ sub add_ExternalFeatureFactory{
=cut
sub get_adaptor() {
-
my ($self, $canonical_name, @other_args) = @_;
- if ($self->isa('Bio::EnsEMBL::Container')) {
- $self = $self->_obj;
- }
+ if ($self->isa('Bio::EnsEMBL::Container')) {
+ $self = $self->_obj;
+ }
# throw if module for $canonical_name does not exist
- $self->throw("No such data type $canonical_name") if (!exists($self->{'current_module'}->{$canonical_name}));
+ throw("No such data type $canonical_name")
+ if (!exists($self->{'current_module'}->{$canonical_name}));
# get module name for $canonical_name
my $module_name = $self->{'default_module'}->{$canonical_name};
# create and store a new one if necessary
if (!exists($self->{'current_objects'}->{$canonical_name})) {
- $self->{'current_objects'}->{$canonical_name} = $self->_get_adaptor($module_name, @other_args);
+ $self->{'current_objects'}->{$canonical_name} =
+ $self->_get_adaptor($module_name, @other_args);
}
return $self->{'current_objects'}->{$canonical_name};
@@ -1200,34 +1108,35 @@ sub get_adaptor() {
Arg [2] : Object defining the adaptor for arg1.
Example : $pa = Bio::EnsEMBL::DBSQL::ProteinAdaptor->new($db_adaptor);
: $db_adaptor->set_adaptor("Protein", $pa)
- Description: Stores the object which represents the adaptor for the arg1 data type.
+ Description: Stores the object which represents the adaptor for the
+ arg1 data type.
Returntype : none
- Exceptions : If arg2 is not a subclass of the default module for this data type.
+ Exceptions : If arg2 is not a subclass of the default module for this
+ data type.
Caller : external
=cut
sub set_adaptor() {
+ my ($self, $canonical_name, $new_object) = @_;
- my ($self, $canonical_name, $new_object) = @_;
-
- if ($self->isa('Bio::EnsEMBL::Container')) {
- $self = $self->_obj;
- }
+ if ($self->isa('Bio::EnsEMBL::Container')) {
+ $self = $self->_obj;
+ }
# throw if an unrecognised canonical_name is used
- $self->throw("No such data type $canonical_name") if (!exists($self->{'default_module'}->{$canonical_name}));
+ throw("No such data type $canonical_name")
+ if(!exists($self->{'default_module'}->{$canonical_name}));
- my $default_module = $self->{'default_module'}->{$canonical_name};
-
- # Check that $new_module is a subclass of $default_module
- if (!$new_object->isa($default_module)) { # polymorphism should work
- $self->throw("ref($new_object) is not a subclass of $default_module");
- }
+ my $default_module = $self->{'default_module'}->{$canonical_name};
- # set the value in current_module
- $self->{'current_objects'}->{$canonical_name} = $new_object;
+ # Check that $new_module is a subclass of $default_module
+ if (!$new_object->isa($default_module)) { # polymorphism should work
+ throw("ref($new_object) is not a subclass of $default_module");
+ }
+ # set the value in current_module
+ $self->{'current_objects'}->{$canonical_name} = $new_object;
}
#
@@ -1236,10 +1145,12 @@ sub set_adaptor() {
=head2 get_GenericFeatureAdaptors
- Arg [1] : List of names of feature adaptors to get. If no adaptor names are given, all the defined adaptors are returned.
+ Arg [1] : List of names of feature adaptors to get. If no
+ adaptor names are given, all the defined adaptors are returned.
Example : $db->get_GenericFeature("SomeFeature", "SomeOtherFeature")
- Description: Returns a hash containing the named feature adaptors (or all feature adaptors).
- Returntype : reference to a Hash containing the named
+ Description: Returns a hash containing the named feature adaptors (or
+ all feature adaptors).
+ Returntype : reference to a Hash containing the named
feature adaptors (or all feature adaptors).
Exceptions : If any of the the named generic feature adaptors do not exist.
Caller : external
@@ -1257,22 +1168,24 @@ sub get_GenericFeatureAdaptors() {
} else {
foreach my $name (@names) {
if (!exists($self->{'generic_feature_adaptors'}->{$name})) {
- $self->throw("No generic feature adaptor has been defined for $name" );
+ throw("No generic feature adaptor has been defined for $name" );
}
$adaptors{$name} = $self->{'generic_feature_adaptors'}->{$name};
}
}
return \%adaptors;
-
}
+
=head2 add_GenericFeatureAdaptor
Arg [1] : The name of the feature.
Arg [2] : Adaptor object for a generic feature.
- Example : $db->add_GenericFeatureAdaptor("SomeFeature", "Bio::EnsEMBL::DBSQL::SomeFeatureAdaptor")
- Description: Stores the object which represents the adaptor for the named feature type.
+ Example : $db->add_GenericFeatureAdaptor("SomeFeature",
+ "Bio::EnsEMBL::DBSQL::SomeFeatureAdaptor")
+ Description: Stores the object which represents the adaptor for the
+ named feature type.
Returntype : none
Exceptions :
Caller : external
@@ -1280,29 +1193,137 @@ sub get_GenericFeatureAdaptors() {
=cut
sub add_GenericFeatureAdaptor() {
-
- my ($self, $name, $adaptor_obj) = @_;
+ my ($self, $name, $adaptor_obj) = @_;
- # check that $adaptor is an object that subclasses BaseFeatureAdaptor
- if (!$adaptor_obj->isa("Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor")) {
- $self->throw("$name is a " . ref($adaptor_obj) . " which is not a subclass of Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor" );
- }
-
- $self->{'generic_feature_adaptors'}->{$name} = $adaptor_obj;
+ # check that $adaptor is an object that subclasses BaseFeatureAdaptor
+ if (!$adaptor_obj->isa("Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor")) {
+ throw("$name is a " . ref($adaptor_obj) . "which is not a " .
+ "subclass of Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor" );
+ }
+ $self->{'generic_feature_adaptors'}->{$name} = $adaptor_obj;
}
-#
+
+
+
+#########################
# sub DEPRECATED METHODS
-#
-#
+#########################
+
+
+
+=head2 get_MapFragAdaptor
+
+ Description: MapFragAdaptor is deprecated. Use MiscFeatureAdaptor instead.
+
+=cut
+
+sub get_MapFragAdaptor {
+ my $self = shift;
+
+ return $self->get_adaptor( "MapFrag" );
+}
+
+=head2 get_CloneAdaptor
+
+ Description: CloneAdaptor is deprecated. Use SliceAdaptor instead.
+
+=cut
+
+sub get_CloneAdaptor {
+ my( $self ) = @_;
+
+ return $self->dnadb->get_adaptor("Clone");
+}
+
+=head2 get_ChromosomeAdaptor
+
+ Description: ChromosomeAdaptor is deprecated. Use SliceAdaptor instead.
+
+=cut
+
+sub get_ChromosomeAdaptor {
+ my( $self ) = @_;
+
+ return
+ $self->dnadb->get_adaptor("Chromosome");
+}
+
+=head2 get_RawContigAdaptor
+
+ Description: RawContigAdaptor is deprecated. Use SliceAdaptor instead.
+
+=cut
+
+sub get_RawContigAdaptor {
+ my( $self ) = @_;
+
+ return $self->dnadb->get_adaptor("RawContig");
+}
+
+
+=head2 get_ProteinAdaptor
+
+ Description: ProteinAdaptor is deprecated. Use TranslationAdaptor instead
+
+=cut
+
+sub get_ProteinAdaptor {
+ my $self = shift;
+ deprecate("The ProteinAdaptor is deprecated. Use the TranslationAdaptor " .
+ "instead of the ProteinAdaptor and Translation instead of " .
+ "Protein.");
+ return $self->get_adaptor("Protein");
+}
+
sub source {
+ deprecate('Do not use - this method does nothing');
+}
+
+
+=head2 assembly_type
+
+ Description: DEPRECATED - Use CoordSystemAdaptor to obtain default coordinate
+ system instead.
+
+=cut
+
+sub assembly_type{
my $self = shift;
- $self->warn("DBAdaptor::source method is deprecated - do not use" .
- $self->stack_trace_dump);
-
+ deprecate('Use CoordSystemAdaptor::fetch_top_level instead');
+
+ my $csa = $self->get_CoordSystemAdaptor();
+
+ #get the default top-level coord system
+ my ($dbID,$name,$version) = $csa->fetch_top_level();
+
+ return $version;
}
+
+
+=head2 list_supported_assemblies
+
+ Description: DEPRECATED - Use CoordSystemAdaptor to obtain list of top-level
+ coordinate systems instead
+
+=cut
+
+sub list_supported_assemblies {
+ my($self) = @_;
+ deprecate('Use CoordSystemAdaptor::fetch_all_top_level instead');
+
+ my $csa = $self->get_CoordSystemAdaptor();
+ my @versions;
+ foreach my $cs (@{$csa->fetch_all_top_level()}) {
+ my ($dbID, $name, $version) = @$csa;
+ push @versions, $version;
+ }
+ return @versions;
+}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/DBConnection.pm b/modules/Bio/EnsEMBL/DBSQL/DBConnection.pm
index 87a2bdb483..611e6a07fa 100644
--- a/modules/Bio/EnsEMBL/DBSQL/DBConnection.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/DBConnection.pm
@@ -1,5 +1,4 @@
-
-=head1 NAME - Bio::EnsEMBL::DBSQL::DBConnection
+#=Head1 NAME - Bio::EnsEMBL::DBSQL::DBConnection
=head1 SYNOPSIS
@@ -45,6 +44,7 @@ use Bio::EnsEMBL::Container;
use Bio::EnsEMBL::Root;
use DBI;
+use Bio::EnsEMBL::Utils::Exception qw(throw info warning);
@ISA = qw(Bio::EnsEMBL::Root);
@@ -102,8 +102,8 @@ sub new {
)],@_);
- $db || $self->throw("Database object must have a database name");
- $user || $self->throw("Database object must have a user");
+ $db || throw("Database object must have a database name");
+ $user || throw("Database object must have a user");
if( ! $driver ) {
$driver = 'mysql';
@@ -122,7 +122,7 @@ sub new {
$dbh = DBI->connect("$dsn","$user",$password, {RaiseError => 1});
};
- $dbh || $self->throw("Could not connect to database $db user " .
+ $dbh || throw("Could not connect to database $db user " .
"$user using [$dsn] as a locator\n" . $DBI::errstr);
$self->db_handle($dbh);
@@ -331,9 +331,9 @@ sub locator {
sub _get_adaptor {
my( $self, $module, @args) = @_;
- if ($self->isa('Bio::EnsEMBL::Container')) {
- $self = $self->_obj;
- }
+ if ($self->isa('Bio::EnsEMBL::Container')) {
+ $self = $self->_obj;
+ }
my( $adaptor, $internal_name );
@@ -348,7 +348,7 @@ sub _get_adaptor {
eval "require $module";
if($@) {
- $self->warn("$module cannot be found.\nException $@\n");
+ warning("$module cannot be found.\nException $@\n");
return undef;
}
@@ -402,13 +402,13 @@ sub prepare {
my ($self,$string) = @_;
if( ! $string ) {
- $self->throw("Attempting to prepare an empty SQL query.");
+ throw("Attempting to prepare an empty SQL query.");
}
if( !defined $self->{_db_handle} ) {
- $self->throw("Database object has lost its database handle.");
+ throw("Database object has lost its database handle.");
}
- #print STDERR "\n\nSQL(".$self->dbname."):$string\n\n";
+ info("SQL(".$self->dbname."):$string");
return $self->{_db_handle}->prepare($string);
}
@@ -433,7 +433,7 @@ sub add_db_adaptor {
my ($self, $name, $adaptor) = @_;
unless($name && $adaptor && ref $adaptor) {
- $self->throw('adaptor and name arguments are required');
+ throw('adaptor and name arguments are required');
}
#avoid circular references and memory leaks
@@ -576,7 +576,6 @@ sub DESTROY {
#don't disconnect if the InactiveDestroy flag has been set
#this can really screw up forked processes
if(!$dbh->{'InactiveDestroy'}) {
- #print STDERR "Disconnecting db\n";
$dbh->disconnect;
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/DBEntryAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/DBEntryAdaptor.pm
index 8eaf2e60f8..c39e855183 100644
--- a/modules/Bio/EnsEMBL/DBSQL/DBEntryAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/DBEntryAdaptor.pm
@@ -15,22 +15,31 @@ MySQL Database queries to load and store external object references.
=head1 SYNOPSIS
$db_entry_adaptor = $db_adaptor->get_DBEntryAdaptor();
-$dbEntry = $db_entry_adaptor->fetch_by_dbID($id);
+$db_entry = $db_entry_adaptor->fetch_by_dbID($id);
+
+my $gene = $db_adaptor->get_GeneAdaptor->fetch_by_stable_id('ENSG00000101367');
+@db_entries = @{$db_entry_adaptor->fetch_all_by_Gene($gene)};
+@gene_ids = $db_entry_adaptor->list_gene_ids_by_extids('BAB15482');
+
=head1 CONTACT
Post questions to the EnsEMBL developer list <ensembl-dev@ebi.ac.uk>
+=head1 METHODS
+
=cut
package Bio::EnsEMBL::DBSQL::DBEntryAdaptor;
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
-use Bio::EnsEMBL::DBSQL::DBAdaptor;
+
use Bio::EnsEMBL::DBEntry;
use Bio::EnsEMBL::IdentityXref;
use Bio::EnsEMBL::GoXref;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate throw);
+
use vars qw(@ISA);
use strict;
@@ -95,14 +104,21 @@ sub fetch_by_dbID {
=head2 store
- Arg [1] : ?? $exObj
- Arg [2] : ?? $ensObject
- Arg [3] : ?? $ensType
- Example :
- Description:
- Returntype :
- Exceptions :
- Caller :
+ Arg [1] : Bio::EnsEMBL::DBEntry $exObj
+ The DBEntry (xref) to be stored
+ Arg [2] : Bio::EnsEMBL::Transcript, Bio::EnsEMBL::Gene or
+ Bio::EnsEMBL::Translation $ensObject
+ An EnsEMBL object to associate with this external database entry
+ Arg [3] : string $ensType ('Transcript', 'Translation', 'Gene');
+ The type of EnsEMBL object that this external database entry is
+ being associated with.
+ Example : $dbea->store($db_entry, $transcript, 'Transcript');
+ Description: Stores a reference to an external database (if it is not stored
+ already) and associates an EnsEMBL object of a specified type
+ with the external identifier.
+ Returntype : int - the identifier of the newly created external refernce
+ Exceptions : none
+ Caller : scripts which load Xrefs and ObjectXrefs, etc. into EnsEMBL.
=cut
@@ -125,7 +141,7 @@ sub store {
my ($dbRef) = $sth->fetchrow_array();
if(!$dbRef) {
- $self->throw("external_db [" . $exObj->dbname . "] release [" .
+ throw("external_db [" . $exObj->dbname . "] release [" .
$exObj->release . "] does not exist");
}
@@ -216,34 +232,12 @@ sub store {
# If its GoXref add the linkage type to go_xref table
#
if ($exObj->isa('Bio::EnsEMBL::IdentityXref')) {
-
- my $analysis_id;
- if( $exObj->analysis() ) {
- $analysis_id = $self->db()->get_AnalysisAdaptor()->
- store( $exObj->analysis() );
- } else {
- $analysis_id = undef;
- }
-
$sth = $self->prepare( "
INSERT ignore INTO identity_xref
SET object_xref_id = ?,
query_identity = ?,
- target_identity = ?,
- query_start = ?,
- query_end = ?,
- translation_start = ?,
- translation_end = ?,
- cigar_line = ?,
- score = ?,
- evalue = ?,
- analysis_id = ?" );
-
- $sth->execute($Xidt, $exObj->query_identity, $exObj->target_identity,
- $exObj->query_start(), $exObj->query_end(),
- $exObj->translation_start(), $exObj->translation_end(),
- $exObj->cigar_line(), $exObj->score(), $exObj->evalue(),
- $analysis_id);
+ target_identity = ?" );
+ $sth->execute($Xidt, $exObj->query_identity, $exObj->target_identity);
} elsif( $exObj->isa( 'Bio::EnsEMBL::GoXref' )) {
$sth = $self->prepare( "
INSERT ignore INTO go_xref
@@ -275,7 +269,7 @@ sub exists {
my ($self, $dbe) = @_ ;
unless($dbe && ref $dbe && $dbe->isa('Bio::EnsEMBL::DBEntry')) {
- $self->throw("arg must be a Bio::EnsEMBL::DBEntry not [$dbe]");
+ throw("arg must be a Bio::EnsEMBL::DBEntry not [$dbe]");
}
my $sth = $self->prepare('SELECT x.xref_id
@@ -402,10 +396,10 @@ sub _fetch_by_object_type {
my @out;
if (!defined($ensObj)) {
- $self->throw("Can't _fetch_by_object_type without an object ID");
+ throw("Can't fetch_by_EnsObject_type without an object");
}
if (!defined($ensType)) {
- $self->throw("Can't _fetch_by_object_type without a type");
+ throw("Can't fetch_by_EnsObject_type without a type");
}
my $sth = $self->prepare("
SELECT xref.xref_id, xref.dbprimary_acc, xref.display_label, xref.version,
@@ -413,9 +407,7 @@ sub _fetch_by_object_type {
exDB.db_name, exDB.release, exDB.status,
oxr.object_xref_id,
es.synonym,
- idt.query_identity, idt.target_identity, idt.query_start, idt.query_end,
- idt.translation_start, idt.translation_end, idt.cigar_line,
- idt.score, idt.evalue, idt.analysis_id,
+ idt.query_identity, idt.target_identity,
gx.linkage_type
FROM xref xref, external_db exDB, object_xref oxr
LEFT JOIN external_synonym es on es.xref_id = xref.xref_id
@@ -434,18 +426,16 @@ sub _fetch_by_object_type {
while ( my $arrRef = $sth->fetchrow_arrayref() ) {
my ( $refID, $dbprimaryId, $displayid, $version,
$desc, $dbname, $release, $exDB_status, $objid,
- $synonym, $queryid, $targetid, $query_start, $query_end,
- $translation_start, $translation_end, $cigar_line,
- $score, $evalue, $analysis_id, $linkage_type ) = @$arrRef;
+ $synonym, $queryid, $targetid, $linkage_type ) = @$arrRef;
my %obj_hash = (
- _adaptor => $self,
- _dbID => $refID,
- _primary_id => $dbprimaryId,
- _display_id => $displayid,
- _version => $version,
- _release => $release,
- _dbname => $dbname);
+ 'adaptor' => $self,
+ 'dbID' => $refID,
+ 'primary_id' => $dbprimaryId,
+ 'display_id' => $displayid,
+ 'version' => $version,
+ 'release' => $release,
+ 'dbname' => $dbname );
# using an outer join on the synonyms as well as on identity_xref, we
@@ -459,20 +449,6 @@ sub _fetch_by_object_type {
$exDB = Bio::EnsEMBL::IdentityXref->new_fast(\%obj_hash);
$exDB->query_identity($queryid);
$exDB->target_identity($targetid);
- if( $analysis_id ) {
- my $analysis = $self->db()->get_AnalysisAdaptor()->
- fetch_by_dbID( $analysis_id );
- if( $analysis ) {
- $exDB->analysis( $analysis );
- }
- }
- $exDB->cigar_line( $cigar_line );
- $exDB->query_start( $query_start );
- $exDB->query_end( $query_end );
- $exDB->translation_start( $translation_start );
- $exDB->translation_end( $translation_end );
- $exDB->score( $score );
- $exDB->evalue( $evalue );
} elsif( defined $linkage_type ) {
$exDB = Bio::EnsEMBL::GoXref->new_fast( \%obj_hash );
$exDB->add_linkage_type( $linkage_type );
@@ -509,10 +485,11 @@ sub _fetch_by_object_type {
=head2 list_gene_ids_by_extids
Arg [1] : string $external_id
- Example : none
- Description: Retrieve a list of geneid by an external identifier that is linked to
- any of the genes transcripts, translations or the gene itself
- Returntype : listref of strings
+ Example : @gene_ids = $dbea->list_gene_ids_by_extids('ARSE');
+ Description: Retrieve a list of geneid by an external identifier that is
+ linked to any of the genes transcripts, translations or the
+ gene itself
+ Returntype : list of ints
Exceptions : none
Caller : unknown
@@ -528,32 +505,16 @@ sub list_gene_ids_by_extids{
return keys %T;
}
-=head2 geneids_by_extids
-
- Arg [1] : string $external_id
- Example : none
- Description: Retrieve a list of geneid by an external identifier that is linked to
- any of the genes transcripts, translations or the gene itself
- (please not that this call is deprecated)
- Returntype : listref of strings
- Exceptions : none
- Caller : unknown
-
-=cut
-sub geneids_by_extids{
- my ($self,$name) = @_;
- warn ("This method is deprecated please use 'list_gene_ids_by_extids");
- return $self->list_gene_ids_by_extids( $name );
-}
=head2 list_transcript_ids_by_extids
Arg [1] : string $external_id
- Example : none
- Description: Retrieve a list transcriptid by an external identifier that is linked to
- any of the genes transcripts, translations or the gene itself
- Returntype : listref of strings
+ Example : @tr_ids = $dbea->list_gene_ids_by_extids('BCRA2');
+ Description: Retrieve a list transcript ids by an external identifier that
+ is linked to any of the genes transcripts, translations or the
+ gene itself
+ Returntype : list of ints
Exceptions : none
Caller : unknown
@@ -570,48 +531,10 @@ sub list_transcript_ids_by_extids{
}
-=head2 transcriptids_by_extids
-
- Arg [1] : string $external_id
- Example : none
- Description: Retrieve a list transcriptid by an external identifier that is linked to
- any of the genes transcripts, translations or the gene itself
- Returntype : listref of strings
- Exceptions : none
- Caller : unknown
-
-=cut
-
-sub transcriptids_by_extids{
- my ($self,$name) = @_;
- warn ("This method is deprecated please use 'list_transcript_ids_by_extids");
- return $self->list_transcript_ids_by_extids( $name );
-}
-
-
-=head2 translationids_by_extids
-
- Arg [1] : string $name
- Example : none
- Description: Gets a list of translation IDs by external display IDs
- (please note that this call is deprecated)
- Returntype : list of Ints
- Exceptions : none
- Caller : unknown
-
-=cut
-
-sub translationids_by_extids{
- my ($self,$name) = @_;
- warn ("This method is deprecated please use 'list_translation_ids_by_extids");
- return $self->list_translation_ids_by_extids( $name );
-}
-
-
=head2 list_translation_ids_by_extids
Arg [1] : string $name
- Example : none
+ Example : @tr_ids = $dbea->list_gene_ids_by_extids('GO:0004835');
Description: Gets a list of translation IDs by external display IDs
Returntype : list of Ints
Exceptions : none
@@ -649,9 +572,20 @@ sub _type_by_external_id{
my $where_sql = '';
my $ID_sql = "oxr.ensembl_id";
if(defined $extraType) {
- $ID_sql = "t.${extraType}_id";
- $from_sql = 'transcript as t, ';
- $where_sql = 't.'.lc($ensType).'_id = oxr.ensembl_id and ';
+ if(lc($extraType) eq 'translation') {
+ $ID_sql = "tl.translation_id";
+ } else {
+ $ID_sql = "t.${extraType}_id";
+ }
+
+ if(lc($ensType) eq 'translation') {
+ $from_sql = 'transcript as t, translation as tl, ';
+ $where_sql = 't.transcript_id = tl.transcript_id and ' .
+ 'tl.translation_id = oxr.ensembl_id and ';
+ } else {
+ $from_sql = 'transcript as t, ';
+ $where_sql = 't.'.lc($ensType).'_id = oxr.ensembl_id and ';
+ }
}
my @queries = (
"select $ID_sql
@@ -684,4 +618,45 @@ sub _type_by_external_id{
}
+
+
+=head2 geneids_by_extids
+
+ Description: DEPRECATED use list_gene_ids_by_extids instead
+
+=cut
+
+sub geneids_by_extids{
+ my ($self,$name) = @_;
+ deprecate(" use 'list_gene_ids_by_extids instead");
+ return $self->list_gene_ids_by_extids( $name );
+}
+
+
+=head2 translationids_by_extids
+
+ Description: DEPRECATED use list_translation_ids_by_extids instead
+
+=cut
+
+sub translationids_by_extids{
+ my ($self,$name) = @_;
+ deprecate("Use list_translation_ids_by_extids instead");
+ return $self->list_translation_ids_by_extids( $name );
+}
+
+
+=head2 transcriptids_by_extids
+
+ Description: DEPRECATED use transcriptids_by_extids instead
+
+=cut
+
+sub transcriptids_by_extids{
+ my ($self,$name) = @_;
+ deprecate("Use list_transcript_ids_by_extids instead.");
+ return $self->list_transcript_ids_by_extids( $name );
+}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/DensityFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/DensityFeatureAdaptor.pm
new file mode 100644
index 0000000000..46358ee1b1
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DBSQL/DensityFeatureAdaptor.pm
@@ -0,0 +1,432 @@
+#
+# Ensembl module for Bio::EnsEMBL::DBSQL::DensityFeatureAdaptor
+#
+# Copyright EMBL/EBI
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::DBSQL::DensityFeatureAdaptor
+
+=head1 SYNOPSIS
+
+my $dfa = $database_adaptor->get_DensityFeatureAdaptor();
+
+my $interpolate = 1;
+my $blocks_wanted = 50;
+
+@dense_feats = @{$dfa->fetch_all_by_Slice($slice,'SNPDensity',
+ $blocks_wanted, $interpolate);}
+
+=head1 DESCRIPTION
+
+Density Feature Adaptor - An adaptor responsible for the creation of density
+features from the database.
+
+=head1 CONTACT
+
+Post questions to the Ensembl developer list.
+
+=head1 METHODS
+
+=cut
+
+package Bio::EnsEMBL::DBSQL::DensityFeatureAdaptor;
+use vars qw(@ISA);
+use strict;
+
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
+use Bio::EnsEMBL::DensityFeature;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
+
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
+
+
+
+=head2 fetch_all_by_Slice
+
+ Arg [1] : Bio::EnsEMBL::Slice $slice - The slice representing the region
+ to retrieve density features from.
+ Arg [2] : string $logic_name - The logic name of the density features to
+ retrieve.
+ Arg [3] : int $num_blocks (optional; default = 50) - The number of
+ features that are desired. The ratio between the size of these
+ features and the size of the features in the database will be
+ used to determine which database features will be used.
+ Arg [4] : boolean $interpolate (optional; default = 0) - A flag indicating
+ whether the features in the database should be interpolated to
+ fit them to the requested number of features. If true the
+ features will be interpolated to provide $num_blocks features.
+ This will not guarantee that exactly $num_blocks features are
+ returned due to rounding etc. but it will be close.
+ Arg [5] : float $max_ratio - The maximum ratio between the size of the
+ requested features (as determined by $num_blocks) and the actual
+ size of the features in the database. If this value is exceeded
+ then an empty list will be returned. This can be used to
+ prevent excessive interpolation of the database values.
+ Example : #interpolate:
+ $feats = $dfa->fetch_all_by_Slice($slice,'SNPDensity', 10, 1);
+ #do not interpoloate, get what is in the database:
+ $feats = $dfa->fetch_all_by_Slice($slice,'SNPDensity', 50);
+ #interpolate, but not too much
+ $feats = $dfa->fetch_all_by_Slice($slice,'SNPDensity',50,1,5.0);
+ Description: Retrieves a set of density features which overlap the region
+ of this slice. Density features are a discrete representation
+ of a continuous value along a sequence, such as a density or
+ percent coverage. Density Features may be stored in chunks of
+ different sizes in the database, and interpolated to fit the
+ desired size for the requested region. For example the database
+ may store a single density value for each 1KB and also for each
+ 1MB. When fetching for an entire chromosome the 1MB density
+ chunks will be used if the requested number of blocks is not
+ very high.
+ Returntype : Bio::EnsEMBL::DensityFeature
+ Exceptions : warning on invalid $num_blocks argument
+ warning if no features with logic_name $logic_name exist
+ warning if density_type table has invalid block_size value
+ Caller : general
+
+=cut
+
+sub fetch_all_by_Slice {
+ my ($self, $slice, $logic_name, $num_blocks, $interpolate, $max_ratio) = @_;
+
+ if(defined($num_blocks) && $num_blocks < 1) {
+ warning("Invalid number of density blocks [$num_blocks] requested.\n" .
+ "Returning empty list.");
+ return [];
+ }
+
+ $num_blocks ||= 50;
+ my $length = $slice->length();
+ my $wanted_block_size = int($length/$num_blocks);
+
+ my $analysis_adaptor = $self->db()->get_AnalysisAdaptor();
+ my $analysis = $analysis_adaptor->fetch_by_logic_name($logic_name);
+
+ if(!$analysis) {
+ warning("No Analysis with logic_name=[$logic_name] found.\n" .
+ 'Returning empty list.');
+ return [];
+ }
+
+ my $sth = $self->prepare
+ ("SELECT density_type_id, block_size, analysis_id, value_type ".
+ "FROM density_type " .
+ "WHERE analysis_id = ?");
+ $sth->execute($analysis->dbID());
+
+ my $best_ratio = undef;
+ my ($density_type_id, $density_value_type, $row);
+
+ while($row = $sth->fetchrow_arrayref) {
+ my $block_size = $row->[1];
+
+ if($block_size < 1) {
+ warning("density_type table contains invalid block_size=$block_size.");
+ next;
+ }
+
+ my $ratio;
+ if($block_size < $wanted_block_size) {
+ $ratio = $wanted_block_size/$block_size;
+ } else {
+ $ratio = $block_size/$wanted_block_size;
+ }
+
+ if(!defined($best_ratio) || $ratio < $best_ratio) {
+ $best_ratio = $ratio;
+ $density_type_id = $row->[0];
+ $density_value_type = $row->[3];
+ }
+ }
+
+ #the ratio was not good enough, or this logic name was not in the
+ #density type table, return an empty list
+ if(!defined($best_ratio) ||
+ (defined($max_ratio) && $best_ratio > $max_ratio)) {
+ return [];
+ }
+
+ my $constraint = "dt.density_type_id = $density_type_id";
+
+ my @features =
+ @{$self->fetch_all_by_Slice_constraint($slice,$constraint)};
+
+ #we don't want to interpolate if the ratio was very close
+ $interpolate = 0 if($best_ratio < 1.05);
+
+ return \@features if(!$interpolate);
+
+ #interpolate the features into new features of a different size
+ my @out;
+ #sort the features on start position
+ @features = sort({$a->start() <=> $b->end()} @features);
+
+ #resize the features that were returned
+ my $start = 1;
+ my $end = $start+$wanted_block_size-1;
+
+ while($start < $length) {
+ $end = $length if($end > $length);
+
+ my $density_value = 0.0;
+ my ($f, $fstart, $fend, $portion);
+ my @dvalues;
+
+ #construct a new feature using all of the old density features that
+ #we overlapped
+ while(($f = shift(@features)) && $end > $f->{'start'}) {
+
+ #what portion of this feature are we using to construct our new block?
+ $fstart = ($f->{'start'} < $start) ? $start : $f->{'start'};
+ $fend = ($f->{'end'} > $end ) ? $end : $f->{'end'};
+ $fend = $length if($fend > $length);
+
+ if($density_value_type eq 'sum') {
+
+ $portion = ($fend-$fstart+1)/$f->length();
+
+ #take a percentage of density value, depending on how much of the
+ #feature we overlapped
+ $density_value += $portion * $f->{'density_value'};
+
+ } elsif($density_value_type eq 'ratio') {
+
+ #maintain a running total of the length used from each feature
+ #and its value
+ push(@dvalues,[$fend-$fstart+1,$f->{'density_value'}]);
+
+ } else {
+ throw("Unknown density value type [$density_value_type].");
+ }
+
+ #do not want to look at next feature if we only used part of this one:
+ last if($fend < $f->{'end'});
+ }
+
+ #if we did not completely overlap the last feature, put it back on so
+ #it can be partially used by the next block
+ if($fend < $f->{'end'}) {
+ unshift(@features, $f);
+ }
+
+ if($density_value_type eq 'ratio') {
+ #take a weighted average of the all the density values of the features
+ #used to construct this one
+ my $total_len = $end - $start + 1;
+ if($total_len > 0) {
+ foreach my $pair (@dvalues) {
+ my ($dlen, $dval) = @$pair;
+ $density_value += $dval * ($dlen/$total_len);
+ }
+ }
+ }
+
+ push @out, Bio::EnsEMBL::DensityFeature->new_fast
+ ({'start' => $start,
+ 'end' => $end,
+ 'slice' => $slice,
+ 'analysis' => $analysis,
+ 'adaptor' => $self,
+ 'density_value' => $density_value,
+ 'density_value_type' => $density_value_type});
+
+ $start = $end + 1;
+ $end += $wanted_block_size;
+ }
+
+ return \@out;
+}
+
+
+sub _tables {
+ my $self = shift;
+
+ return (['density_feature', 'df'], ['density_type', 'dt']);
+}
+
+
+sub _columns {
+ my $self = shift;
+
+ return qw( df.density_feature_id
+ df.seq_region_id df.seq_region_start df.seq_region_end
+ df.density_value dt.analysis_id dt.value_type);
+}
+
+
+sub _default_where_clause {
+ my $self = shift;
+
+ return 'df.density_type_id = dt.density_type_id';
+}
+
+
+sub _objs_from_sth {
+ my ($self, $sth, $mapper, $dest_slice) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches,
+ # and a fair bit of gymnastics have been used.
+ #
+
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $aa = $self->db()->get_AnalysisAdaptor();
+
+ my @features;
+ my %analysis_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my($density_feature_id, $seq_region_id, $seq_region_start, $seq_region_end,
+ $density_value, $analysis_id, $density_value_type );
+
+ $sth->bind_columns(\$density_feature_id, \$seq_region_id, \$seq_region_start,
+ \$seq_region_end, \$density_value, \$analysis_id,
+ \$density_value_type);
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
+
+ FEATURE: while($sth->fetch()) {
+ #get the analysis object
+ my $analysis = $analysis_hash{$analysis_id} ||=
+ $aa->fetch_by_dbID($analysis_id);
+
+ #get the slice object
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ my $len = $seq_region_end - $seq_region_start + 1;
+
+ my @coords =
+ $mapper->map($sr_name, $seq_region_start, $seq_region_end,1, $sr_cs);
+
+ #filter out gaps
+ @coords = grep {!$_->isa('Bio::EnsEMBL::Mapper::Gap')} @coords;
+
+ #throw out density features mapped to gaps, or split
+ next FEATURE if(@coords != 1);
+
+ $seq_region_start = $coords[0]->{'start'};
+ $seq_region_end = $coords[0]->{'end'};
+ $sr_name = $coords[0]->{'id'};
+
+ if($density_value_type eq 'sum') {
+ #adjust density value so it reflects length of feature actually used
+ my $newlen = $seq_region_end - $seq_region_start +1;
+ $density_value *= $newlen/$len if($newlen != $len);
+ }
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ }
+
+ #throw away features entirely off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+ $slice = $dest_slice;
+ }
+
+ push @features, Bio::EnsEMBL::DensityFeature->new_fast(
+ {'start' => $seq_region_start,
+ 'end' => $seq_region_end,
+ 'slice' => $slice,
+ 'analysis' => $analysis,
+ 'adaptor' => $self,
+ 'dbID' => $density_feature_id,
+ 'density_value' => $density_value,
+ 'density_value_type' => $density_value_type});
+ }
+
+ return \@features;
+}
+
+
+=head2 list_dbIDs
+
+ Arg [1] : none
+ Example : @feature_ids = @{$density_feature_adaptor->list_dbIDs()};
+ Description: Gets an array of internal ids for all density features in the
+ current db
+ Returntype : list of ints
+ Exceptions : none
+ Caller : ?
+
+=cut
+
+sub list_dbIDs {
+ my ($self) = @_;
+
+ return $self->_list_dbIDs("density_feature");
+}
+
+1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/DnaAlignFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/DnaAlignFeatureAdaptor.pm
index af5677da75..7e9f1a97ff 100644
--- a/modules/Bio/EnsEMBL/DBSQL/DnaAlignFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/DnaAlignFeatureAdaptor.pm
@@ -1,14 +1,10 @@
#
-# BioPerl module for Bio::EnsEMBL::DBSQL::DnaAlignFeatureAdaptor
+# EnsEMBL module for Bio::EnsEMBL::DnaAlignFeatureAdaptor
#
-# Cared for by Ewan Birney <birney@ebi.ac.uk>
-#
-# Copyright Ewan Birney
+# Copyright (c) 2003 EnsEMBL
#
# You may distribute this module under the same terms as perl itself
-# POD documentation - main docs before the code
-
=head1 NAME
Bio::EnsEMBL::DBSQL::DnaAlignFeatureAdaptor - Adaptor for DnaAlignFeatures
@@ -17,22 +13,18 @@ Bio::EnsEMBL::DBSQL::DnaAlignFeatureAdaptor - Adaptor for DnaAlignFeatures
$dafa = $dbadaptor->get_DnaAlignFeatureAdaptor();
- @features = @{$dafa->fetch_by_RawContig($contig)};
+ @features = @{$dafa->fetch_by_Slice($slice)};
$dafa->store(@features);
=head1 DESCRIPTION
+This is an adaptor responsible for the retrieval and storage of
+DnaDnaAlignFeatures from the database. This adaptor inherits most of its
+functionality from the BaseAlignFeatureAdaptor and BaseFeatureAdaptor
+superclasses.
-This is an adaptor for DNA features on DNA sequence. Like other
-feature getting adaptors it has a number of fetch_ functions and a
-store function. This adaptor inherits most of its functionality from
-its BaseAlignFeatureAdaptor superclass.
-
-
-=head1 AUTHOR - Ewan Birney
-
-Email birney@ebi.ac.uk
+=head1 CONTACT
Post questions to the EnsEMBL development list <ensembl-dev@ebi.ac.uk>
@@ -45,6 +37,7 @@ use strict;
use Bio::EnsEMBL::DnaDnaAlignFeature;
use Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(throw);
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor);
@@ -84,79 +77,112 @@ sub _columns {
my $self = shift;
#warning, implementation of _objs_from_sth method depends on order of list
- return qw(daf.dna_align_feature_id
- daf.contig_id
- daf.analysis_id
- daf.contig_start
- daf.contig_end
- daf.contig_strand
- daf.hit_start
- daf.hit_end
- daf.hit_name
- daf.hit_strand
- daf.cigar_line
- daf.evalue
- daf.perc_ident score);
+ return qw(daf.dna_align_feature_id
+ daf.seq_region_id
+ daf.analysis_id
+ daf.seq_region_start
+ daf.seq_region_end
+ daf.seq_region_strand
+ daf.hit_start
+ daf.hit_end
+ daf.hit_name
+ daf.hit_strand
+ daf.cigar_line
+ daf.evalue
+ daf.perc_ident
+ daf.score);
}
=head2 store
- Arg [1] : int $contig_id
- the id of the contig to be stored in the database
- Arg [2] : list of Bio::EnsEMBL::DnaAlignFeatures @sf
+ Arg [1] : list of Bio::EnsEMBL::DnaAlignFeatures @feats
the features to store in the database
- Example : $dna_align_feature_adaptor->store($contig_id, @features);
+ Example : $dna_align_feature_adaptor->store(@features);
Description: Stores a list of DnaAlignFeatures in the database
Returntype : none
- Exceptions : thrown if $contig_id is not an int, or if any of the
- features in the database are not storable
- Caller : ?
+ Exceptions : throw if any of the provided features cannot be stored
+ which may occur if:
+ * The feature does not have an associate Slice
+ * The feature does not have an associated analysis
+ * The Slice the feature is associated with is on a seq_region
+ unknown to this database
+ A warning is given if:
+ * The feature has already been stored in this db
+ Caller : Pipeline
=cut
sub store {
- my ($self, @sf) = @_;
+ my ($self, @feats) = @_;
+
+ throw("Must call store with features") if( scalar(@feats) == 0 );
my @tabs = $self->_tables;
my ($tablename) = @{$tabs[0]};
-
- if( scalar(@sf) == 0 ) {
- $self->throw("Must call store with sequence features");
- }
-
- my $sth = $self->prepare("
- INSERT INTO $tablename (contig_id, contig_start, contig_end,
- contig_strand, hit_start, hit_end,
+
+ my $db = $self->db();
+ my $analysis_adaptor = $db->get_AnalysisAdaptor();
+
+ my $sth = $self->prepare(
+ "INSERT INTO $tablename (seq_region_id, seq_region_start, seq_region_end,
+ seq_region_strand, hit_start, hit_end,
hit_strand, hit_name, cigar_line,
- analysis_id, score, evalue, perc_ident)
+ analysis_id, score, evalue, perc_ident)
VALUES (?,?,?,?,?,?,?,?,?,?,?, ?, ?)");
- foreach my $sf ( @sf ) {
- if( !ref $sf || !$sf->isa("Bio::EnsEMBL::DnaDnaAlignFeature") ) {
- $self->throw("feature must be a Bio::EnsEMBL::DnaDnaAlignFeature,"
- . " not a [$sf]");
+ FEATURE: foreach my $feat ( @feats ) {
+ if( !ref $feat || !$feat->isa("Bio::EnsEMBL::DnaDnaAlignFeature") ) {
+ throw("feature must be a Bio::EnsEMBL::DnaDnaAlignFeature,"
+ . " not a [".ref($feat)."].");
+ }
+
+ if($feat->is_stored($db)) {
+ warning("DnaDnaAlignFeature [".$feat->dbID."] is already stored" .
+ " in this database.");
+ next FEATURE;
+ }
+
+ my $hstart = $feat->hstart();
+ my $hend = $feat->hend();
+ my $hstrand = $feat->hstrand();
+ $self->_check_start_end_strand($hstart,$hend, $hstrand);
+
+ my $cigar_string = $feat->cigar_string();
+ if(!$cigar_string) {
+ $cigar_string = $feat->length() . 'M';
+ warning("DnaDnaAlignFeature does not define a cigar_string.\n" .
+ "Assuming ungapped block with cigar_line=$cigar_string .");
}
-
- my $contig = $sf->entire_seq();
- unless(defined $contig && $contig->isa("Bio::EnsEMBL::RawContig")) {
- $self->throw("A contig must be attached to the features to be " .
- "stored via the attach seq method\n");
+
+ my $hseqname = $feat->hseqname();
+ if(!$hseqname) {
+ throw("DnaDnaAlignFeature must define an hseqname.");
+ }
+
+ if(!defined($feat->analysis)) {
+ throw("An analysis must be attached to the features to be stored.");
}
- if( !defined $sf->analysis ) {
- $self->throw("Cannot store sequence features without analysis");
+ #store the analysis if it has not been stored yet
+ if(!$feat->analysis->is_stored($db)) {
+ $analysis_adaptor->store($feat->analysis());
}
- # will only store if object is not already stored in this database
- $self->db()->get_AnalysisAdaptor()->store( $sf->analysis() );
+ my $original = $feat;
+ my $seq_region_id;
+ ($feat, $seq_region_id) = $self->_pre_store($feat);
+
+ $sth->execute( $seq_region_id, $feat->start, $feat->end, $feat->strand,
+ $hstart, $hend, $hstrand, $hseqname,
+ $cigar_string, $feat->analysis->dbID, $feat->score,
+ $feat->p_value, $feat->percent_id);
- $sth->execute( $contig->dbID(), $sf->start, $sf->end, $sf->strand,
- $sf->hstart, $sf->hend, $sf->hstrand, $sf->hseqname,
- $sf->cigar_string, $sf->analysis->dbID, $sf->score,
- $sf->p_value, $sf->percent_id);
- $sf->dbID($sth->{'mysql_insertid'});
+ $original->dbID($sth->{'mysql_insertid'});
+ $original->adaptor($self);
}
+
+ $sth->finish();
}
@@ -176,120 +202,143 @@ sub store {
=cut
sub _objs_from_sth {
- my ($self, $sth, $mapper, $slice) = @_;
-
- my ($dna_align_feature_id, $contig_id, $analysis_id, $contig_start,
- $contig_end, $contig_strand, $hit_start, $hit_end, $hit_name,
- $hit_strand, $cigar_line, $evalue, $perc_ident, $score);
-
- my $rca = $self->db()->get_RawContigAdaptor();
- my $aa = $self->db()->get_AnalysisAdaptor();
-
- my ($analysis, $contig);
- my @features;
+ my ($self, $sth, $mapper, $dest_slice) = @_;
- my %a_hash;
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
- my ($row, $row_cache);
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $aa = $self->db->get_AnalysisAdaptor();
- $row_cache = $sth->fetchall_arrayref();
-
- if($slice) {
- my ($chr, $start, $end, $strand);
- my $slice_start = $slice->chr_start();
- my $slice_end = $slice->chr_end();
- my $slice_strand = $slice->strand();
- my $slice_name = $slice->name();
+ my @features;
+ my %analysis_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my($dna_align_feature_id, $seq_region_id, $analysis_id, $seq_region_start,
+ $seq_region_end, $seq_region_strand, $hit_start, $hit_end, $hit_name,
+ $hit_strand, $cigar_line, $evalue, $perc_ident, $score);
+
+ $sth->bind_columns(
+ \$dna_align_feature_id, \$seq_region_id, \$analysis_id, \$seq_region_start,
+ \$seq_region_end, \$seq_region_strand, \$hit_start, \$hit_end, \$hit_name,
+ \$hit_strand, \$cigar_line, \$evalue, \$perc_ident, \$score);
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
- my ($feat_start, $feat_end, $feat_strand);
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
- while($row = shift @$row_cache) {
- ($dna_align_feature_id, $contig_id, $analysis_id, $contig_start,
- $contig_end, $contig_strand, $hit_start, $hit_end, $hit_name,
- $hit_strand, $cigar_line, $evalue, $perc_ident, $score) = @$row;
+ FEATURE: while($sth->fetch()) {
+ #get the analysis object
+ my $analysis = $analysis_hash{$analysis_id} ||=
+ $aa->fetch_by_dbID($analysis_id);
- #convert contig coordinates to assembly coordinates
- ($chr, $start, $end, $strand) =
- $mapper->fast_to_assembly($contig_id, $contig_start,
- $contig_end, $contig_strand);
-
- #if mapped to gap, skip
- next unless(defined $start);
+ #get the slice object
+ my $slice = $slice_hash{"ID:".$seq_region_id};
- #if mapped outside slice region, skip
- next if ($start > $slice_end) || ($end < $slice_start);
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
- #convert assembly coordinates to slice coordinates
- if($slice_strand == -1) {
- $feat_start = $slice_end - $end + 1;
- $feat_end = $slice_end - $start + 1;
- $feat_strand = $strand * -1;
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
} else {
- $feat_start = $start - $slice_start + 1;
- $feat_end = $end - $slice_start + 1;
- $feat_strand = $strand;
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
}
-
- $analysis = $a_hash{$analysis_id} ||= $aa->fetch_by_dbID($analysis_id);
-
- push @features, Bio::EnsEMBL::DnaDnaAlignFeature->new_fast(
- {'_gsf_tag_hash' => {},
- '_gsf_sub_array' => [],
- '_parse_h' => {},
- '_analysis' => $analysis,
- '_gsf_start' => $feat_start,
- '_gsf_end' => $feat_end,
- '_gsf_strand' => $feat_strand,
- '_gsf_score' => $score,
- '_seqname' => $slice_name,
- '_percent_id' => $perc_ident,
- '_p_value' => $evalue,
- '_hstart' => $hit_start,
- '_hend' => $hit_end,
- '_hstrand' => $hit_strand,
- '_hseqname' => $hit_name,
- '_gsf_seq' => $slice,
- '_cigar_string' => $cigar_line,
- '_id' => $hit_name,
- '_database_id' => $dna_align_feature_id});
}
- } else {
- my %c_hash;
- while($row = shift @$row_cache) {
- ($dna_align_feature_id, $contig_id, $analysis_id, $contig_start,
- $contig_end, $contig_strand, $hit_start, $hit_end, $hit_name,
- $hit_strand, $cigar_line, $evalue, $perc_ident, $score) = @$row;
-
- $analysis = $a_hash{$analysis_id} ||= $aa->fetch_by_dbID($analysis_id);
- $contig = $c_hash{$contig_id} ||= $rca->fetch_by_dbID($contig_id);
-
- #use a very fast (hack) constructor since we may be creating over 10000
- #features at a time and normal object construction is too slow.
- push @features, Bio::EnsEMBL::DnaDnaAlignFeature->new_fast(
- {'_gsf_tag_hash' => {},
- '_gsf_sub_array' => [],
- '_parse_h' => {},
- '_analysis' => $analysis,
- '_gsf_start' => $contig_start,
- '_gsf_end' => $contig_end,
- '_gsf_strand' => $contig_strand,
- '_gsf_score' => $score,
- '_seqname' => $contig->name,
- '_percent_id' => $perc_ident,
- '_p_value' => $evalue,
- '_hstart' => $hit_start,
- '_hend' => $hit_end,
- '_hstrand' => $hit_strand,
- '_hseqname' => $hit_name,
- '_gsf_seq' => $contig,
- '_cigar_string' => $cigar_line,
- '_id' => $hit_name,
- '_database_id' => $dna_align_feature_id});
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+ $slice = $dest_slice;
}
-
+
+ #finally, create the new dna align feature
+ push @features, Bio::EnsEMBL::DnaDnaAlignFeature->new_fast
+ ( { 'slice' => $slice,
+ 'start' => $seq_region_start,
+ 'end' => $seq_region_end,
+ 'strand' => $seq_region_strand,
+ 'hseqname' => $hit_name,
+ 'hstart' => $hit_start,
+ 'hend' => $hit_end,
+ 'hstrand' => $hit_strand,
+ 'score' => $score,
+ 'p_value' => $evalue,
+ 'percent_id' => $perc_ident,
+ 'cigar_string' => $cigar_line,
+ 'analysis' => $analysis,
+ 'adaptor' => $self,
+ 'dbID' => $dna_align_feature_id } );
}
-
+
return \@features;
}
@@ -297,7 +346,8 @@ sub _objs_from_sth {
Arg [1] : none
Example : @feature_ids = @{$dna_align_feature_adaptor->list_dbIDs()};
- Description: Gets an array of internal ids for all dna align features in the current db
+ Description: Gets an array of internal ids for all dna align features in
+ the current db
Returntype : list of ints
Exceptions : none
Caller : ?
diff --git a/modules/Bio/EnsEMBL/DBSQL/ExonAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/ExonAdaptor.pm
index 2f6ebc4d81..0c8f62e090 100644
--- a/modules/Bio/EnsEMBL/DBSQL/ExonAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/ExonAdaptor.pm
@@ -36,60 +36,76 @@ use vars qw( @ISA );
use strict;
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
use Bio::EnsEMBL::Exon;
-use Bio::EnsEMBL::StickyExon;
+use Bio::EnsEMBL::Utils::Exception qw( warning throw deprecate );
+
+@ISA = qw( Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor );
-@ISA = qw( Bio::EnsEMBL::DBSQL::BaseAdaptor );
-=head2 fetch_by_dbID
+#_tables
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns the names, aliases of the tables to use for queries
+# Returntype : list of listrefs of strings
+# Exceptions : none
+# Caller : internal
+
+sub _tables {
+ my $self = shift;
- Arg [1] : int $exon_id
- the unique internal id of the exon to retrieve
- Example : $exon = $exon_adaptor->fetch_by_dbID($exon_id);
- Description: Retrieves an exon from the database via its internal id
- Returntype : Bio::EnsEMBL::Exon in contig coordinates
- Exceptions : none
- Caller : general
+ ##allow the table definition to be overridden by certain methods
+ return ($self->{'tables'}) ?
+ @{$self->{'tables'}} :
+ ([ 'exon', 'e' ], [ 'exon_stable_id', 'esi' ] );
+}
-=cut
-sub fetch_by_dbID {
+#=head2 _columns
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns a list of columns to use for queries
+# Returntype : list of strings
+# Exceptions : none
+# Caller : internal
+
+sub _columns {
my $self = shift;
- my $dbID = shift;
-
- my $query = qq {
- SELECT e.exon_id
- , e.contig_id
- , e.contig_start
- , e.contig_end
- , e.contig_strand
- , e.phase
- , e.end_phase
- , e.sticky_rank
- FROM exon e
- WHERE e.exon_id = $dbID
- ORDER BY e.sticky_rank DESC };
-
- my $sth = $self->prepare($query);
+ return qw( e.exon_id e.seq_region_id e.seq_region_start e.seq_region_end
+ e.seq_region_strand e.phase e.end_phase
+ esi.stable_id esi.version );
+}
- $sth->execute();
+sub _left_join {
+ return ( [ 'exon_stable_id', "esi.exon_id = e.exon_id" ]);
+}
- my $hashRef;
- my $exon;
- if( $hashRef = $sth->fetchrow_hashref() ) {
- $exon = $self->_exon_from_sth( $sth, $hashRef );
- }
- delete $self->{rchash};
- return $exon;
+# _final_clause
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns a default end for the SQL-query (ORDER BY)
+# Returntype : string
+# Exceptions : none
+# Caller : internal
+
+sub _final_clause {
+ my $self = shift;
+ return $self->{'final_clause'} || '';
}
+
=head2 fetch_by_stable_id
Arg [1] : string $stable_id
@@ -103,202 +119,60 @@ sub fetch_by_dbID {
=cut
sub fetch_by_stable_id {
- my $self = shift;
- my $stable_id = shift;
-
- my $sth = $self->prepare( "SELECT exon_id from exon_stable_id
- WHERE stable_id = ?" );
- $sth->execute( $stable_id );
- if( my $arr = $sth->fetchrow_arrayref ) {
- my $exon = $self->fetch_by_dbID( $arr->[0] );
- return $exon;
- } else {
- $self->warn( "No Exon with this stable_id in the database!" );
- return undef;
- }
-}
-
-
-
-=head2 fetch_all_by_gene_id
-
- Arg [1] : int $id
- The identifier of the gene whose exons will be retrieved
- Example : @exons = $exon_adaptor->fetch_all_by_gene_id(1234);
- Description: Retrieves all exons from the gene specified by $geneId
- Returntype : listref of Bio::EnsEMBL::Exon in contig coordinates
- Exceptions : thrown if $geneId is not defined
- Caller : general
-
-=cut
-
-sub fetch_all_by_gene_id {
- my ( $self, $gene_id ) = @_;
- my %exons;
- my $hashRef;
- my ( $currentId, $currentTranscript );
+ my ( $self, $stable_id ) = @_;
- if( !$gene_id ) {
- $self->throw("Gene dbID not defined");
- }
- $self->{rchash} = {};
- my $query = qq {
- SELECT
- STRAIGHT_JOIN
- e.exon_id
- , e.contig_id
- , e.contig_start
- , e.contig_end
- , e.contig_strand
- , e.phase
- , e.end_phase
- , e.sticky_rank
- FROM transcript t
- , exon_transcript et
- , exon e
- WHERE t.gene_id = ?
- AND et.transcript_id = t.transcript_id
- AND e.exon_id = et.exon_id
- ORDER BY t.transcript_id,e.exon_id
- , e.sticky_rank DESC
- };
+ my $constraint = "esi.stable_id = \"$stable_id\"";
- my $sth = $self->prepare( $query );
- $sth->execute($gene_id);
-
- while( $hashRef = $sth->fetchrow_hashref() ) {
- if( ! exists $exons{ $hashRef->{exon_id} } ) {
-
- my $exon = $self->_exon_from_sth( $sth, $hashRef );
-
- $exons{$exon->dbID} = $exon;
- }
- }
- delete $self->{rchash};
-
- my @out = ();
+ # should be only one :-)
+ my $exons = $self->SUPER::generic_fetch( $constraint );
- push @out, values %exons;
+ if( ! @$exons ) { return undef }
- return \@out;
+ return $exons->[0];
}
-=head2 _exon_from_sth
-
- Arg [1] : DBI statement handle $sth
- the prepared SQL statement used to generate the hashref
- this arg is required to retrieve component exons from
- the database if the exon happens to be a sticky exon
- Arg [2] : DBI row hashref $hashRef
- a hash reference representing a single exon or a portion
- of a sticky exon
- Example : my $exon = $self->_exon_from_sth($sth, $hashref);
- Description: PROTECTED
- Creates an exon (normal or sticky) from its statement handle
- and hashreference.
- Returntype : Bio::EnsEMBL::Exon
+=head2 fetch_all_by_Transcript
+
+ Arg [1] : Bio::EnsEMBL::Transcript $transcript
+ Example : none
+ Description: Retrieves all Exons for the Transcript in 5-3 order
+ Returntype : listref Bio::EnsEMBL::Exon on Transcript slice
Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::ExonAdaptor
+ Caller : Transcript->get_all_Exons()
=cut
+sub fetch_all_by_Transcript {
+ my ( $self, $transcript ) = @_;
-# build an exon (possibly sticky exon) from given statement handle and
-# retrieved row in hashref. return it. uses $self->{rchash} to store contig
-# information. This should be cleared after leaving ExonAdaptor
-
-sub _exon_from_sth {
-
- my ( $self, $sth, $hashRef ) = @_;
- my $sticky_length = 0;
- my $exon;
- if( $hashRef->{'sticky_rank'} >1 ) {
-
- # sticky exon
- $exon = Bio::EnsEMBL::StickyExon->new();
- $exon->dbID($hashRef->{'exon_id'});
- # make first component exon
- my $component = $self->_new_Exon_from_hashRef($hashRef);
-
- $exon->add_component_Exon($component);
- $sticky_length += $component->length;
-
- $exon->phase($component->phase);
- $exon->end_phase($component->end_phase);
- $exon->adaptor($self);
-
- # continue while loop until we hit sticky_rank 1
- while( $hashRef = $sth->fetchrow_hashref() ) {
- my $component = $self->_new_Exon_from_hashRef($hashRef);
-
- $exon->add_component_Exon($component);
- $sticky_length += $component->length;
-
- if( $component->sticky_rank == 1 ) {
- $exon->contig( $component->contig );
- last;
- }
- }
+ ##override the tables definition to provide an additional join to
+ # the exon_transcript table. For efficiency we cannot afford to have
+ # this in as a left join every time.
+ my @tables = $self->_tables();
+ push @tables, ['exon_transcript', 'et'];
+ $self->{'tables'} = \@tables;
+ $self->{'final_clause'} = "ORDER BY et.transcript_id, et.rank";
- $exon->_sort_by_sticky_rank();
- # set start = 1 and end = length of sticky exon
- # build a minature sequence representing the sticky region and
- # attach
+ my $constraint = "et.transcript_id = ".$transcript->dbID() .
+ " AND e.exon_id = et.exon_id";
- $exon->start(1);
- $exon->end($sticky_length);
- $exon->strand( 1 );
+ #fetch exons, remap them to the transcripts slice, and
+ # keep even exons which fall off end of slice
+ my $keep_all = 1;
+ my $exons = $self->generic_fetch( $constraint, undef,
+ $transcript->slice(), $keep_all);
- }
- else {
- $exon = $self->_new_Exon_from_hashRef($hashRef);
- }
+ #un-override the table definition
+ $self->{'tables'} = undef;
+ $self->{'final_clause'} = undef;
- return $exon;
+ return $exons;
}
-=head2 _new_Exon_from_hashref
-
- Arg [1] : DBI row hashref $hashRef
- Example : my $exon = $hashref
- Description: PROTECTED
- creates a normal (non-sticky) exon from a row hash reference
- Returntype : Bio::EnsEMBL::Exon
- Exceptions : none
- Caller : _exon_from_sth
-
-=cut
-
-sub _new_Exon_from_hashRef {
- my $self = shift;
- my $hashRef = shift;
-
- my $exon = Bio::EnsEMBL::Exon->new();
- $exon->start( $hashRef->{'contig_start'} );
- $exon->end( $hashRef->{'contig_end'} );
- $exon->strand( $hashRef->{'contig_strand'} );
- $exon->phase( $hashRef->{phase} );
- $exon->end_phase( $hashRef->{end_phase} );
- $exon->dbID($hashRef->{'exon_id'});
- $exon->sticky_rank($hashRef->{'sticky_rank'});
- $exon->adaptor($self);
-
- my $rc =
- $self->db->get_RawContigAdaptor->fetch_by_dbID($hashRef->{'contig_id'});
-
- $exon->contig( $rc );
-
- return $exon;
-}
-
-
-
-
-
=head2 store
Arg [1] : Bio::EnsEMBL::Exon $exon
@@ -317,199 +191,100 @@ sub store {
my ( $self, $exon ) = @_;
if( ! $exon->isa('Bio::EnsEMBL::Exon') ) {
- $self->throw("$exon is not a EnsEMBL exon - not dumping!");
+ throw("$exon is not a EnsEMBL exon - not storing.");
}
- if( $exon->dbID && $exon->adaptor && $exon->adaptor == $self ) {
+ my $db = $self->db();
+
+ if($exon->is_stored($db)) {
return $exon->dbID();
}
if( ! $exon->start || ! $exon->end ||
! $exon->strand || ! defined $exon->phase ) {
- $self->throw("Exon does not have all attributes to store");
+ throw("Exon does not have all attributes to store");
}
- # trap contig_id separately as it is likely to be a common mistake
-
my $exon_sql = q{
- INSERT into exon ( exon_id, contig_id, contig_start,
- contig_end, contig_strand, phase,
- end_phase, sticky_rank )
- VALUES ( ?, ?, ?, ?, ?, ?, ?,? )
+ INSERT into exon ( seq_region_id, seq_region_start,
+ seq_region_end, seq_region_strand, phase,
+ end_phase )
+ VALUES ( ?, ?, ?, ?, ?, ? )
};
+
my $exonst = $self->prepare($exon_sql);
my $exonId = undef;
- if( $exon->isa( 'Bio::EnsEMBL::StickyExon' )) {
- # sticky storing. Sticky exons contain normal exons ...
-
- my $componentExons = $exon->get_all_component_Exons;
- for my $componentExon ( @$componentExons ) {
- my $contig = $componentExon->contig();
-
- unless($contig && ref $contig && $contig->dbID()) {
- $self->throw("Component Exon does not have an attached contig " .
- "with a valid set database id. " .
- "Needs to have one set");
- }
-
- $exonst->execute( $exonId, $contig->dbID,
- $componentExon->start(),
- $componentExon->end(),
- $componentExon->strand(),
- $componentExon->phase(),
- $componentExon->end_phase(),
- $componentExon->sticky_rank() );
- if( ! $exonId ) {
- $exonId = $exonst->{'mysql_insertid'};
- }
- }
- } else {
- # normal storing
-
- my $contig = $exon->contig();
-
- unless( $contig && ref $contig && $contig->dbID() ) {
- $self->throw("Exon does not have an attached contig with a valid " .
- "database id. Needs to have one set");
- }
-
- $exonst->execute( undef,$contig->dbID,
- $exon->start(),
- $exon->end(),
- $exon->strand(),
- $exon->phase(),
- $exon->end_phase(),
- $exon->sticky_rank() );
- $exonId = $exonst->{'mysql_insertid'};
+ my $original = $exon;
+ my $seq_region_id;
+ ($exon, $seq_region_id) = $self->_pre_store($exon);
+
+ #store the exon
+ $exonst->execute( $seq_region_id,
+ $exon->start(),
+ $exon->end(),
+ $exon->strand(),
+ $exon->phase(),
+ $exon->end_phase());
+ $exonId = $exonst->{'mysql_insertid'};
+
+ #store any stable_id information
+ if ($exon->stable_id && $exon->version()) {
+ my $sth = $self->prepare(
+ "INSERT INTO exon_stable_id " .
+ "SET version = ?, " .
+ "stable_id = ?, " .
+ "exon_id = ?");
+
+ $sth->execute( $exon->version, $exon->stable_id, $exonId );
}
- if ($exon->stable_id) {
- if (!$exon->created ||
- !$exon->modified ||
- !$exon->version) {
- $self->throw("Trying to store incomplete stable id information for exon");
- }
-
- my $statement = "INSERT INTO exon_stable_id(exon_id," .
- "version, stable_id, created, modified)".
- " VALUES(" . $exonId . "," .
- $exon->version . "," .
- "'" . $exon->stable_id . "'," .
- "FROM_UNIXTIME(".$exon->created."),".
- "FROM_UNIXTIME(". $exon->modified . "))";
- #print $statement . "\n";
-
- my $sth = $self->prepare($statement);
- $sth->execute();
- }
-
# Now the supporting evidence
# should be stored from featureAdaptor
my $sql = "insert into supporting_feature (exon_id, feature_id, feature_type)
- values(?, ?, ?)";
-
- my $sf_sth = $self->db->prepare($sql);
+ values(?, ?, ?)";
+
+ my $sf_sth = $self->prepare($sql);
my $anaAdaptor = $self->db->get_AnalysisAdaptor();
my $dna_adaptor = $self->db->get_DnaAlignFeatureAdaptor();
my $pep_adaptor = $self->db->get_ProteinAlignFeatureAdaptor();
my $type;
- my @exons = ();
- if($exon->isa('Bio::EnsEMBL::StickyExon')) {
- @exons = @{$exon->get_all_component_Exons};
- } else {
- @exons = ($exon);
- }
-
- foreach my $e (@exons) {
- foreach my $sf (@{$e->get_all_supporting_features}) {
- unless($sf->isa("Bio::EnsEMBL::BaseAlignFeature")){
- $self->throw("$sf must be an align feature otherwise" .
- "it can't be stored");
- }
-
- #sanity check
- eval { $sf->validate(); };
- if ($@) {
- $self->warn("Supporting feature invalid. Skipping feature\n");
- next;
- }
-
- $sf->contig($e->contig);
-
- if($sf->isa("Bio::EnsEMBL::DnaDnaAlignFeature")){
- $dna_adaptor->store($sf);
- $type = 'dna_align_feature';
- }elsif($sf->isa("Bio::EnsEMBL::DnaPepAlignFeature")){
- $pep_adaptor->store($sf);
- $type = 'protein_align_feature';
- } else {
- $self->warn("Supporting feature of unknown type. Skipping : [$sf]\n");
- next;
- }
+ foreach my $sf (@{$exon->get_all_supporting_features}) {
+ if(!$sf->isa("Bio::EnsEMBL::BaseAlignFeature")){
+ throw("$sf must be an align feature otherwise" .
+ "it can't be stored");
+ }
- $sf_sth->execute($exonId, $sf->dbID, $type);
+ if($sf->isa("Bio::EnsEMBL::DnaDnaAlignFeature")){
+ $dna_adaptor->store($sf);
+ $type = 'dna_align_feature';
+ }elsif($sf->isa("Bio::EnsEMBL::DnaPepAlignFeature")){
+ $pep_adaptor->store($sf);
+ $type = 'protein_align_feature';
+ } else {
+ warning("Supporting feature of unknown type. Skipping : [$sf]\n");
+ next;
}
+
+ $sf_sth->execute($exonId, $sf->dbID, $type);
}
#
# Finally, update the dbID and adaptor of the exon (and any component exons)
# to point to the new database
#
- foreach my $e (@exons) {
- $e->dbID($exonId);
- $e->adaptor($self);
- }
-
- $exon->adaptor($self);
- $exon->dbID($exonId);
-}
-
-
-=head2 get_stable_entry_info
- Arg [1] : Bio::EnsEMBL::Exon $exon
- Example : $exon_adaptor->get_stable_entry_info($exon);
- Description: gets stable info for an exon. this is not usually done at
- creation time for speed purposes, and can be lazy-loaded later
- if it is needed..
- Returntype : none
- Exceptions : none
- Caller : Bio::EnsEMBL::Exon
-
-=cut
-
-sub get_stable_entry_info {
- my ($self,$exon) = @_;
+ $original->adaptor($self);
+ $original->dbID($exonId);
- if( !$exon || !ref $exon || !$exon->isa('Bio::EnsEMBL::Exon') ) {
- $self->throw("Needs a exon object, not a $exon");
- }
- if(!$exon->dbID){
- #$self->throw("can't fetch stable info with no dbID");
- return;
- }
- my $sth = $self->prepare("SELECT stable_id, UNIX_TIMESTAMP(created),
- UNIX_TIMESTAMP(modified), version
- FROM exon_stable_id
- WHERE exon_id = " . $exon->dbID);
+ return $exonId;
+}
- $sth->execute();
- # my @array = $sth->fetchrow_array();
- if( my $aref = $sth->fetchrow_arrayref() ) {
- $exon->{'_stable_id'} = $aref->[0];
- $exon->{'_created'} = $aref->[1];
- $exon->{'_modified'} = $aref->[2];
- $exon->{'_version'} = $aref->[3];
- }
-
- return 1;
-}
=head2 remove
@@ -565,8 +340,8 @@ sub remove {
$sth = $self->prepare( "delete from supporting_feature where exon_id = ?" );
$sth->execute( $exon->dbID );
- # uhh, didnt know another way of resetting to undef ...
- $exon->{dbID} = undef;
+ $exon->dbID(undef);
+ $exon->adaptor(undef);
}
=head2 list_dbIDs
@@ -603,4 +378,271 @@ sub list_stable_ids {
return $self->_list_dbIDs("exon_stable_id", "stable_id");
}
+#_objs_from_sth
+#
+# Arg [1] : StatementHandle $sth
+# Example : none
+# Description: PROTECTED implementation of abstract superclass method.
+# responsible for the creation of Exons
+# Returntype : listref of Bio::EnsEMBL::Exons in target coordinate system
+# Exceptions : none
+# Caller : internal
+
+sub _objs_from_sth {
+ my ($self, $sth, $mapper, $dest_slice, $keep_all) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
+
+ my $sa = $self->db()->get_SliceAdaptor();
+
+ my @exons;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my ( $exon_id, $seq_region_id, $seq_region_start,
+ $seq_region_end, $seq_region_strand, $phase,
+ $end_phase, $stable_id, $version );
+
+ $sth->bind_columns( \$exon_id, \$seq_region_id,
+ \$seq_region_start,
+ \$seq_region_end, \$seq_region_strand, \$phase,
+ \$end_phase, \$stable_id, \$version );
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ my $dest_slice_cs;
+ my $asma;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ $dest_slice_cs = $dest_slice->coord_system();
+ $asma = $self->db->get_AssemblyMapperAdaptor();
+ }
+
+ FEATURE: while($sth->fetch()) {
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+ my $dest_mapper = $mapper;
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
+
+ #obtain a mapper if none was defined, but a dest_seq_region was
+ if(!$dest_mapper && $dest_slice &&
+ !$dest_slice_cs->equals($slice->coord_system)) {
+ $dest_mapper = $asma->fetch_by_CoordSystems($dest_slice_cs,
+ $slice->coord_system);
+ $asm_cs = $dest_mapper->assembled_CoordSystem();
+ $cmp_cs = $dest_mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($dest_mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $dest_mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE if(!$keep_all);
+ }
+ }
+
+ $slice = $dest_slice;
+ }
+
+ #finally, create the new repeat feature
+ push @exons, Bio::EnsEMBL::Exon->new
+ ( '-start' => $seq_region_start,
+ '-end' => $seq_region_end,
+ '-strand' => $seq_region_strand,
+ '-adaptor' => $self,
+ '-slice' => $slice,
+ '-dbID' => $exon_id,
+ '-stable_id' => $stable_id,
+ '-version' => $version,
+ '-phase' => $phase,
+ '-end_phase' => $end_phase )
+
+ }
+
+ return \@exons;
+}
+
+
+
+
+
+=head2 get_stable_entry_info
+
+ Arg [1] : Bio::EnsEMBL::Exon $exon
+ Example : $exon_adaptor->get_stable_entry_info($exon);
+ Description: gets stable info for an exon. this is not usually done at
+ creation time for speed purposes, and can be lazy-loaded later
+ if it is needed..
+ Returntype : none
+ Exceptions : none
+ Caller : Bio::EnsEMBL::Exon
+
+=cut
+
+sub get_stable_entry_info {
+ my ($self,$exon) = @_;
+
+ deprecated( "This method call shouldnt be necessary" );
+
+ if( !$exon || !ref $exon || !$exon->isa('Bio::EnsEMBL::Exon') ) {
+ $self->throw("Needs a exon object, not a $exon");
+ }
+ if(!$exon->dbID){
+ #$self->throw("can't fetch stable info with no dbID");
+ return;
+ }
+ my $sth = $self->prepare("SELECT stable_id, UNIX_TIMESTAMP(created),
+ UNIX_TIMESTAMP(modified), version
+ FROM exon_stable_id
+ WHERE exon_id = " . $exon->dbID);
+
+ $sth->execute();
+
+ # my @array = $sth->fetchrow_array();
+ if( my $aref = $sth->fetchrow_arrayref() ) {
+ $exon->{'_stable_id'} = $aref->[0];
+ $exon->{'_created'} = $aref->[1];
+ $exon->{'_modified'} = $aref->[2];
+ $exon->{'_version'} = $aref->[3];
+ }
+
+ return 1;
+}
+
+=head2 fetch_all_by_gene_id
+
+ Description: DEPRECATED. This method should not be needed - Exons can
+ be fetched by Transcript.
+
+=cut
+
+sub fetch_all_by_gene_id {
+ my ( $self, $gene_id ) = @_;
+ my %exons;
+ my $hashRef;
+ my ( $currentId, $currentTranscript );
+
+ deprecated( "Hopefully this method is not needed any more. Exons should be fetched by Transcript" );
+
+ if( !$gene_id ) {
+ $self->throw("Gene dbID not defined");
+ }
+ $self->{rchash} = {};
+ my $query = qq {
+ SELECT
+ STRAIGHT_JOIN
+ e.exon_id
+ , e.contig_id
+ , e.contig_start
+ , e.contig_end
+ , e.contig_strand
+ , e.phase
+ , e.end_phase
+ , e.sticky_rank
+ FROM transcript t
+ , exon_transcript et
+ , exon e
+ WHERE t.gene_id = ?
+ AND et.transcript_id = t.transcript_id
+ AND e.exon_id = et.exon_id
+ ORDER BY t.transcript_id,e.exon_id
+ , e.sticky_rank DESC
+ };
+
+ my $sth = $self->prepare( $query );
+ $sth->execute($gene_id);
+
+ while( $hashRef = $sth->fetchrow_hashref() ) {
+ if( ! exists $exons{ $hashRef->{exon_id} } ) {
+
+ my $exon = $self->_exon_from_sth( $sth, $hashRef );
+
+ $exons{$exon->dbID} = $exon;
+ }
+ }
+ delete $self->{rchash};
+
+ my @out = ();
+
+ push @out, values %exons;
+
+ return \@out;
+}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/GeneAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/GeneAdaptor.pm
index a341a55414..9666ce09ec 100644
--- a/modules/Bio/EnsEMBL/DBSQL/GeneAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/GeneAdaptor.pm
@@ -1,29 +1,43 @@
-# EnsEMBL Gene reading writing adaptor for mySQL
#
# Copyright EMBL-EBI 2001
#
# Author: Arne Stabenau
-# based on
+# based on
# Elia Stupkas Gene_Obj
-#
+#
# Date : 20.02.2001
#
=head1 NAME
-Bio::EnsEMBL::DBSQL::GeneAdaptor - MySQL Database queries to generate and store gens.
+Bio::EnsEMBL::DBSQL::GeneAdaptor - A database aware adaptor responsible for the
+retrieval and storage of Gene objects.
=head1 SYNOPSIS
-$gene_adaptor = $db_adaptor->get_GeneAdaptor();
+ use Bio::EnsEMBL::DBSQL::DBAdaptor;
+
+ $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+
+ $gene_adaptor = $db->get_GeneAdaptor();
+
+ $gene = $gene_adaptor->fetch_by_dbID(1234);
+
+ $gene = $gene_adaptor->fetch_by_stable_id('ENSG00000184129');
+
+ @genes = @{$gene_adaptor->fetch_all_by_external_name('BRCA2')};
+
+ $slice_adaptor = $db->get_SliceAdaptor;
+ $slice = $slice_adaptor->fetch_by_region('chromosome', '1', 1, 1000000);
+ @genes = @{$gene_adaptor->fetch_all_by_Slice($slice)};
+
=head1 CONTACT
- Arne Stabenau: stabenau@ebi.ac.uk
- Elia Stupka : elia@ebi.ac.uk
- Ewan Birney : birney@ebi.ac.uk
+Contact the EnsEMBL development list for questions or information:
+ensembl-dev@ebi.ac.uk
-=head1 APPENDIX
+=head1 METHODS
=cut
@@ -32,44 +46,66 @@ package Bio::EnsEMBL::DBSQL::GeneAdaptor;
use strict;
+
+use Bio::EnsEMBL::Utils::Exception qw( deprecate throw warning );
+
use Bio::EnsEMBL::DBSQL::SliceAdaptor;
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
use Bio::EnsEMBL::DBSQL::DBAdaptor;
use Bio::EnsEMBL::Gene;
-use Bio::EnsEMBL::Utils::Cache; #CPAN LRU cache
use vars '@ISA';
-@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
-my $SLICE_GENE_CACHE_SIZE = 3;
-=head2 new
- Arg [1] : list of arguments @args
- Example : $gene_adaptor = new Bio::EnsEMBL::DBSQL::GeneAdaptor($db_adptr);
- Description: Craetes a new GeneAdaptor object
- Returntype : Bio::EnsEMBL::DBSQL::GeneAdaptor
- Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::DBAdaptor
+# _tables
+# Arg [1] : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns the names, aliases of the tables to use for queries
+# Returntype : list of listrefs of strings
+# Exceptions : none
+# Caller : internal
-=cut
+sub _tables {
+ my $self = shift;
-sub new {
- my($class, @args) = @_;
+ return ([ 'gene', 'g' ],
+ [ 'gene_description', 'gd' ],
+ [ 'gene_stable_id', 'gsi' ],
+ [ 'xref', 'x' ],
+ [ 'external_db' , 'exdb' ]);
+}
- $class = ref $class || $class;
- #call superclass constructor
- my $self = $class->SUPER::new(@args);
+# _columns
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns a list of columns to use for queries
+# Returntype : list of strings
+# Exceptions : none
+# Caller : internal
+
+sub _columns {
+ my $self = shift;
+
+ return qw( g.gene_id g.seq_region_id g.seq_region_start g.seq_region_end
+ g.seq_region_strand g.analysis_id g.type g.display_xref_id
+ gd.description gsi.stable_id gsi.version x.display_label
+ exdb.db_name exdb.status );
+}
- #initialize tied hash cache
- tie (%{$self->{'_slice_gene_cache'}},
- 'Bio::EnsEMBL::Utils::Cache',
- $SLICE_GENE_CACHE_SIZE,);
- return $self;
+sub _left_join {
+ return ( [ 'gene_description', "gd.gene_id = g.gene_id" ],
+ [ 'gene_stable_id', "gsi.gene_id = g.gene_id" ],
+ [ 'xref', "x.xref_id = g.display_xref_id" ],
+ [ 'external_db', "exdb.external_db_id = x.external_db_id" ] );
}
+
+
=head2 list_dbIDs
Arg [1] : none
@@ -91,8 +127,8 @@ sub list_dbIDs {
Arg [1] : none
Example : @stable_gene_ids = @{$gene_adaptor->list_stable_dbIDs()};
- Description: Gets an array of stable ids for all genes in the current db
- Returntype : list of ints
+ Description: Gets an listref of stable ids for all genes in the current db
+ Returntype : reference to a list of strings
Exceptions : none
Caller : ?
@@ -104,264 +140,82 @@ sub list_stable_ids {
return $self->_list_dbIDs("gene_stable_id", "stable_id");
}
-=head2 list_geneIds
-
- Arg [1] : none
- Example : @gene_ids = $gene_adaptor->list_geneIds();
- Description: DEPRECATED; use list_dbIDs instead
- Returntype : list of ints
- Exceptions : none
- Caller : ?
-
-=cut
-
-sub list_geneIds {
- my ($self) = @_;
-
- $self->warn("list_geneIds is deprecated; use list_dbIDs instead");
- return $self->list_dbIDs();
-}
-
-=head2 list_stable_geneIds
-
- Arg [1] : list_stable_gene_ids
- Example : @stable_ids = $gene_adaptor->list_stable_gene_ids();
- Description: DEPRECATED; use list_stable_dbIDs() instead.
- Returntype : list of strings
- Exceptions : none
- Caller : ?
-
-=cut
-
-sub list_stable_geneIds {
- my ($self) = @_;
-
- $self->warn("list_stable_geneIds is deprecated; use list_stable_dbIDs instead");
- return $self->list_stable_ids();
-
-}
-
-=head2 fetch_by_dbID
-
- Arg [1] : int $geneId
- the unique internal database id of the Gene to be retrieved
- Arg [2] : int $chromosomal_coordinates (optional)
- if defined, try to return chromosomal coordinates.
- Example : $gene = $gene_adaptor->fetch_by_dbID
- Description: Retrieves a gene object from the database using its unique
- internal identifier.
- Returntype : Bio::EnsEMBL::Gene in contig coordinates
- Exceptions : thrown if no exons exist for the gene with dbID $geneId
- Caller : general
-
-=cut
-
-sub fetch_by_dbID {
- my ( $self, $geneId, $chr_coordinates ) = @_;
-
- my $exonAdaptor = $self->db->get_ExonAdaptor();
- my @exons = @{$exonAdaptor->fetch_all_by_gene_id( $geneId )};
- my %exonIds;
-
- #
- # We assumme that if we get no exons this is a throwing situation
- #
- if( scalar( @exons ) == 0 ) {
- $self->throw("No exons for gene $geneId, assumming no gene");
- }
-
- my $transcriptAdaptor = $self->db->get_TranscriptAdaptor();
-
- # fetching all exons by gene
- # fetching all transcripts
- # adding the exons
- # adding the transcripts
- my %transcriptExons;
- my %transcripts;
-
- my $query = qq{
- SELECT tscript.gene_id
- , tscript.transcript_id
- , e_t.exon_id, e_t.rank
- , gene.analysis_id
- , gene.type
- , tscript.translation_id
- FROM gene
- , transcript tscript
- , exon_transcript e_t
- WHERE gene.gene_id = tscript.gene_id
- AND tscript.transcript_id = e_t.transcript_id
- AND gene.gene_id = $geneId
-
- ORDER BY tscript.gene_id
- , tscript.transcript_id
- , e_t.rank
- };
-
- my $sth = $self->prepare( $query );
- $sth->execute();
- my $ana;
- my $first = 1;
- my $gene;
-
- while( my @arr = $sth->fetchrow_array() ) {
- # building a gene
- if( $first ) {
- $gene = Bio::EnsEMBL::Gene->new();
- $gene->adaptor($self);
- $gene->dbID( $geneId );
- $ana = $self->db->get_AnalysisAdaptor->fetch_by_dbID($arr[4]);
- $gene->analysis($ana);
- $gene->type($arr[5]);
- $first = 0;
- }
-
- # store an array of exon ids for each transcript
- if( !exists $transcriptExons{$arr[1]} ) {
- $transcriptExons{$arr[1]} = [];
- }
-
- push( @{$transcriptExons{$arr[1]}}, $arr[2] );
- $transcripts{$arr[1]} = $arr[6];
- }
-
- if( $first ) {
- return undef;
- }
-
- #discard duplicate exons, add analysis object to exons
- foreach my $exon ( @exons ) {
- $exon->analysis($ana);
- $exonIds{$exon->dbID} = $exon;
- }
-
- foreach my $transcriptId ( keys %transcripts ) {
- # should be fetch_by_geneId ..
- my $transcript = Bio::EnsEMBL::Transcript->new();
- $transcript->dbID( $transcriptId );
- $transcript->adaptor( $self->db->get_TranscriptAdaptor() );
-
- # Test for truth because translation_id will be 0 if not set
- # because column is NOT NULL in schema.
- if (my $translation_id = $transcripts{$transcriptId}) {
- $transcript->_translation_id($translation_id);
- }
-
- foreach my $exonId ( @{$transcriptExons{$transcriptId}} ) {
-
- $transcript->add_Exon( $exonIds{$exonId} );
- }
- # store also a type for the transcript
- $transcript->type($gene->type);
- $gene->add_Transcript( $transcript );
- }
-
- # if chromosomal coordinates are needed, transform with empty slice
-
- if( defined $chr_coordinates ) {
- my $sa = $self->db->get_SliceAdaptor();
- my $empty_slice = Bio::EnsEMBL::Slice->new
- (
- -empty => 1,
- -adaptor => $sa
- );
- $gene->transform( $empty_slice );
- }
-
- return $gene;
-}
=head2 fetch_by_stable_id
- Arg [1] : string $id
+ Arg 1 : string $id
The stable id of the gene to retrieve
- Arg [2] : (optional) boolean $chr_coords
- flag indicating genes should be returned in chromosomal
- coords instead of contig coords.
Example : $gene = $gene_adaptor->fetch_by_stable_id('ENSG00000148944');
- Description: Retrieves a gene object from the database via its stable id
- Returntype : Bio::EnsEMBL::Gene in contig coordinates by default or in
- chromosomal coords if the $chr_coords arg is set to 1.
- Exceptions : thrown if no gene of stable_id $id exists in the database
+ Description: Retrieves a gene object from the database via its stable id.
+ The gene will be retrieved in its native coordinate system (i.e.
+ in the coordinate system it is stored in the database). It may
+ be converted to a different coordinate system through a call to
+ transform() or transfer(). If the gene or exon is not found
+ undef is returned instead.
+ Returntype : Bio::EnsEMBL::Gene in given coordinate system
+ Exceptions : if we cant get the gene in given coord system
Caller : general
=cut
-sub fetch_by_stable_id{
- my ($self,$id, $chr_coords) = @_;
+sub fetch_by_stable_id {
+ my ($self,$id) = @_;
- my $sth = $self->prepare("SELECT gene_id
- FROM gene_stable_id
- WHERE stable_id = '$id'");
- $sth->execute;
+ #because of the way this query is constructed (with a left join to the
+ # gene_stable_id table), it is faster to do 2 queries, getting the gene_id
+ # in the first query
- my ($dbID) = $sth->fetchrow_array();
+ my $sth = $self->prepare("SELECT gene_id from gene_stable_id " .
+ "WHERE stable_id = ?");
+ $sth->execute($id);
- if( !defined $dbID ) {
- $self->throw("No stable id with $id, cannot fetch");
- }
+ my ($dbID) = $sth->fetchrow_array();
- my $gene = $self->fetch_by_dbID($dbID);
+ return undef if(!$dbID);
- if($chr_coords) {
- #transform gene to chromosomal coords
- my $slice_adaptor = $self->db->get_SliceAdaptor;
- my $slice = new Bio::EnsEMBL::Slice(-empty => 1,
- -adaptor => $slice_adaptor);
- $gene->transform($slice);
- }
-
- return $gene;
+ return $self->fetch_by_dbID($dbID);
}
=head2 fetch_by_exon_stable_id
Arg [1] : string $id
- The stable id of the gene to retrieve
- Arg [2] : (optional) boolean $chr_coords
- flag indicating genes should be returned in chromosomal
- coords instead of contig coords.
- Example : $gene = $gene_adaptor->fetch_by_exon_stable_id('ENSE00000148944');
- Description: Retrieves a gene object from the database via an exon stable id
- Returntype : Bio::EnsEMBL::Gene in contig coordinates by default or in
- chromosomal coords if the $chr_coords arg is set to 1.
- Exceptions : thrown if no gene of stable_id $id exists in the database
+ The stable id of an exon of the gene to retrieve
+ Example : $gene = $gene_adptr->fetch_by_exon_stable_id('ENSE00000148944');
+ Description: Retrieves a gene object from the database via an exon stable id.
+ The gene will be retrieved in its native coordinate system (i.e.
+ in the coordinate system it is stored in the database). It may
+ be converted to a different coordinate system through a call to
+ transform() or transfer(). If the gene or exon is not found
+ undef is returned instead.
+ Returntype : Bio::EnsEMBL::Gene (or undef)
+ Exceptions : none
Caller : general
=cut
sub fetch_by_exon_stable_id{
- my ($self,$id, $chr_coords) = @_;
+ my ( $self, $id ) = @_;
my $sth = $self->prepare(
"SELECT t.gene_id
FROM transcript as t,
exon_transcript as et,
exon_stable_id as esi
- WHERE t.transcript_id = et.transcript_id and et.exon_id = esi.exon_id and esi.stable_id = '$id'");
- $sth->execute;
+ WHERE t.transcript_id = et.transcript_id
+ AND et.exon_id = esi.exon_id
+ AND esi.stable_id = ?");
+ $sth->execute("$id");
my ($dbID) = $sth->fetchrow_array();
- warn( "GENE: $dbID\n" );
- if( !defined $dbID ) {
- $self->throw("No stable id with $id, cannot fetch");
- }
+ return undef if(!defined($dbID));
- my $gene = $self->fetch_by_dbID($dbID);
+ my $gene = $self->fetch_by_dbID( $dbID );
- if($chr_coords) {
- #transform gene to chromosomal coords
- my $slice_adaptor = $self->db->get_SliceAdaptor;
- my $slice = new Bio::EnsEMBL::Slice(-empty => 1,
- -adaptor => $slice_adaptor);
- $gene->transform($slice);
- }
-
return $gene;
- }
+}
@@ -370,11 +224,12 @@ sub fetch_by_exon_stable_id{
Arg [1] : string $domain
the domain to fetch genes from
- Arg [2] : (optional) boolean $empty_flag
- true if lightweight genes are desired (for speed purposes)
Example : my @genes = $gene_adaptor->fetch_all_by_domain($domain);
Description: retrieves a listref of genes whose translation contain interpro
- domain $domain.
+ domain $domain. The genes are returned in their native coord
+ system (i.e. the coord_system they are stored in). If the coord
+ system needs to be changed, then tranform
+ or transfer should be called on the individual objects returned.
Returntype : list of Bio::EnsEMBL::Genes
Exceptions : none
Caller : domainview
@@ -382,131 +237,47 @@ sub fetch_by_exon_stable_id{
=cut
sub fetch_all_by_domain {
- my ($self, $domain, $empty_flag) = @_;
+ my ($self, $domain) = @_;
unless($domain) {
- $self->throw("domain argument is required");
+ throw("domain argument is required");
}
- my $sth = $self->prepare("SELECT tr.gene_id
- FROM interpro i,
- protein_feature pf,
- transcript tr
- WHERE i.interpro_ac = ?
- AND i.id = pf.hit_id
- AND pf.translation_id = tr.translation_id
- GROUP BY tr.gene_id");
-
- $sth->execute($domain);
- my @gene_ids = ();
- my $gene_id;
+ my $sth = $self->prepare("SELECT tr.gene_id " .
+ "FROM interpro i, " .
+ " protein_feature pf, " .
+ " transcript tr, " .
+ " translation tl " .
+ "WHERE i.interpro_ac = ? " .
+ "AND i.id = pf.hit_id " .
+ "AND pf.translation_id = tl.translation_id ".
+ "AND tr.transcript_id = tl.transcript_id " .
+ "GROUP BY tr.gene_id");
- $sth->bind_columns(\$gene_id);
- while($sth->fetch()) {
- push @gene_ids, $gene_id;
- }
-
- #may want to use proxy...
- return $self->db->get_GeneAdaptor->fetch_all_by_gene_id_list(\@gene_ids,
- $empty_flag);
-}
-
-
-
-=head2 fetch_all_by_Slice
-
- Arg [1] : Bio::EnsEMBL::Slice $slice
- the slice to fetch genes from
- Arg [2]
- Example : $genes = $gene_adaptor->fetch_all_by_Slice($slice);
- Description: Retrieves all genes which are present on a slice
- Returntype : listref of Bio::EnsEMBL::Genes in slice coordinates
- Exceptions : none
- Caller : Bio::EnsEMBL::Slice
-
-=cut
-
-sub fetch_all_by_Slice {
- my ( $self, $slice, $logic_name ) = @_;
-
- my @out = ();
-
- $logic_name ||= '';
-
- my $key = uc($slice->name .":" . $logic_name);
-
- #check the cache which uses the slice name as it key
- if($self->{'_slice_gene_cache'}{$key}) {
- return $self->{'_slice_gene_cache'}{$key};
- }
-
- my $mapper = $self->db->get_AssemblyMapperAdaptor->fetch_by_type
- ( $slice->assembly_type() );
-
- my @cids = $mapper->list_contig_ids( $slice->chr_name(),
- $slice->chr_start(),
- $slice->chr_end());
-
- # no genes found so return
- if ( scalar (@cids) == 0 ) {
- return [];
- }
-
- my $str = "(".join( ",",@cids ).")";
-
- my $where = "WHERE e.contig_id in $str
- AND et.exon_id = e.exon_id
- AND et.transcript_id = t.transcript_id
- AND g.gene_id = t.gene_id";
+ $sth->execute($domain);
- if($logic_name) {
- #determine analysis id via logic_name
- my $analysis =
- $self->db->get_AnalysisAdaptor()->fetch_by_logic_name($logic_name);
- unless(defined($analysis) && $analysis->dbID()) {
- $self->warn("No analysis for logic name $logic_name exists");
- return [];
- }
-
- my $analysis_id = $analysis->dbID;
- $where .= " AND g.analysis_id = $analysis_id";
- }
-
- my $sql = "
- SELECT distinct(t.gene_id)
- FROM transcript t,exon_transcript et,exon e, gene g
- $where";
-
- my $sth = $self->db->prepare($sql);
- $sth->execute;
+ my @array = @{$sth->fetchall_arrayref()};
+ $sth->finish();
- while( my ($geneid) = $sth->fetchrow ) {
- my $gene = $self->fetch_by_dbID( $geneid );
- my $newgene = $gene->transform( $slice );
+ my @gene_ids = map {$_->[0]} @array;
- if( $newgene->start() <= $slice->length() &&
- $newgene->end() >= 1 ) {
- # only take the gene if its really overlapping the Slice
- push( @out, $newgene );
- }
- }
-
- #place the results in an LRU cache
- $self->{'_slice_gene_cache'}{$key} = \@out;
-
- return \@out;
+ return $self->fetch_all_by_dbID_list(\@gene_ids);
}
=head2 fetch_by_transcript_id
- Arg [1] : int $transid
+ Arg [1] : int $transid
unique database identifier for the transcript whose gene should
- be retrieved.
- Example : $gene = $gene_adaptor->fetch_by_transcript_id($transcript);
+ be retrieved. The gene is returned in its native coord
+ system (i.e. the coord_system it is stored in). If the coord
+ system needs to be changed, then tranform or transfer should
+ be called on the returned object. undef is returned if the
+ gene or transcript is not found in the database.
+ Example : $gene = $gene_adaptor->fetch_by_transcript_id( 1241 );
Description: Retrieves a gene from the database via the database identifier
- of one of its transcripts
+ of one of its transcripts.
Returntype : Bio::EnsEMBL::Gene
Exceptions : none
Caller : ?
@@ -514,26 +285,28 @@ sub fetch_all_by_Slice {
=cut
sub fetch_by_transcript_id {
- my $self = shift;
- my $transid = shift;
+ my ( $self, $trans_id ) = @_;
# this is a cheap SQL call
- my $sth = $self->prepare(" SELECT tr.gene_id
- FROM transcript as tr
- WHERE tr.transcript_id = $transid");
- $sth->execute;
+ my $sth = $self->prepare("SELECT tr.gene_id " .
+ "FROM transcript as tr " .
+ "WHERE tr.transcript_id = ?");
+ $sth->execute($trans_id);
my ($geneid) = $sth->fetchrow_array();
- if( !defined $geneid ) {
- return undef;
- }
+
+ $sth->finish();
+
+ return undef if( !defined $geneid );
+
my $gene = $self->fetch_by_dbID( $geneid );
return $gene;
}
+
=head2 fetch_by_transcript_stable_id
- Arg [1] : string $transid
+ Arg [1] : string $transid
unique database identifier for the transcript whose gene should
be retrieved.
Example : none
@@ -546,15 +319,15 @@ sub fetch_by_transcript_id {
=cut
sub fetch_by_transcript_stable_id {
- my $self = shift;
- my $transid = shift;
+ my ( $self, $trans_stable_id) = @_;
# this is a cheap SQL call
- my $sth = $self->prepare(" SELECT tr.gene_id
- FROM transcript as tr, transcript_stable_id tcl
- WHERE tcl.stable_id = \"$transid\"
- AND tr.transcript_id = tcl.transcript_id");
- $sth->execute;
+ my $sth = $self->prepare(
+ "SELECT tr.gene_id " .
+ "FROM transcript as tr, transcript_stable_id tcl " .
+ "WHERE tcl.stable_id = ? " .
+ "AND tr.transcript_id = tcl.transcript_id");
+ $sth->execute("$trans_stable_id");
my ($geneid) = $sth->fetchrow_array();
if( !defined $geneid ) {
@@ -566,32 +339,36 @@ sub fetch_by_transcript_stable_id {
-=head2 fetch_by_Peptide_id
- Arg [1] : string $peptideid
+
+=head2 fetch_by_translation_stable_id
+
+ Arg [1] : string $translation_stable_id
the stable id of a translation of the gene that should
be obtained
- Example : $gene = $gene_adaptor->fetch_by_Peptide_id('ENSP00000278194');
- Description: retrieves a gene via the stable id of one of its translations
- this method should be renamed, but for now it stays
- Returntype : Bio::EnsEMBL::Gene in contig coordinates
+ Example : $gene = $gene_adaptor->fetch_by_translation_stable_id
+ ( 'ENSP00000278194' );
+ Description: retrieves a gene via the stable id of one of its translations.
+ Returntype : Bio::EnsEMBL::Gene
Exceptions : none
Caller : geneview
=cut
-sub fetch_by_Peptide_id {
- my $self = shift;
- my $peptideid = shift;
+sub fetch_by_translation_stable_id {
+ my ( $self, $translation_stable_id ) = @_;
# this is a cheap SQL call
- my $sth = $self->prepare(" SELECT tr.gene_id
- FROM transcript as tr,
- translation_stable_id as trs
- WHERE trs.stable_id = '$peptideid'
- AND trs.translation_id = tr.translation_id
- ");
- $sth->execute;
+ my $sth = $self->prepare
+ ("SELECT tr.gene_id " .
+ "FROM transcript as tr, " .
+ " translation as tl, " .
+ " translation_stable_id as trs " .
+ "WHERE trs.stable_id = ? " .
+ "AND trs.translation_id = tl.translation_id " .
+ "AND tr.transcript_id = tl.transcript_id");
+
+ $sth->execute("$translation_stable_id");
my ($geneid) = $sth->fetchrow_array();
if( !defined $geneid ) {
@@ -602,106 +379,192 @@ sub fetch_by_Peptide_id {
-=head2 fetch_by_maximum_DBLink
-
- Title : fetch_by_maximum_DBLink
- Usage : $geneAdaptor->fetch_by_maximum_DBLink($ext_id)
- Function: gets one gene out of the db with
- Returns : gene object (with transcripts, exons)
- Args :
+=head2 fetch_all_by_external_name
+ Arg [1] : string $external_id
+ the external identifier for the gene to be obtained
+ Example : @genes = @{$gene_adaptor->fetch_all_by_external_name('BRCA2')}
+ Description: retrieves a list of genes with an external database
+ idenitifier $external_id. The genes returned are in their
+ native coordinate system. I.e. in the coordinate system they
+ are stored in the database in. If another coordinate system
+ is required then the Gene::transfer or Gene::transform method
+ can be used.
+ Returntype : listref of Bio::EnsEMBL::Genes
+ Exceptions : none
+ Caller : goview
=cut
-sub fetch_by_maximum_DBLink {
- my $self = shift;
- my $external_id = shift;
-
- my $genes=$self->fetch_all_by_DBEntry( $external_id );
-
- my $biggest;
- my $max=0;
- my $size=scalar(@$genes);
- if ($size > 0) {
- foreach my $gene (@$genes) {
- my $size = scalar(@{$gene->get_all_Exons});
- if ($size > $max) {
- $biggest = $gene;
- $max=$size;
- }
- }
- return $biggest;
- }
- return;
+sub fetch_all_by_external_name {
+ my ( $self, $external_id) = @_;
+
+ my $entryAdaptor = $self->db->get_DBEntryAdaptor();
+
+ my @ids = $entryAdaptor->list_gene_ids_by_extids( $external_id );
+
+ return $self->fetch_all_by_dbID_list(\@ids);
}
-=head2 get_stable_entry_info
+
+=head2 fetch_all_alt_alleles
Arg [1] : Bio::EnsEMBL::Gene $gene
- Example : $gene_adaptor->get_stable_entry_info($gene);
- Description: gets stable info for a gene. this is not usually done at
- creation time for speed purposes, and can be lazy-loaded later
- if it is needed..
- Returntype : none
- Exceptions : none
- Caller : Bio::EnsEMBL::Gene
+ Example : my @alt_genes = @{$gene_adaptor->fetch_all_alt_alleles($gene);}
+ foreach my $alt_gene (@alt_genes) {
+ print "Alternate allele: " . $alt_gene->stable_id() . "\n";
+ }
+ Description: Retrieves genes which are alternate alleles to a provided gene.
+ Alternate alleles in Ensembl are genes which are similar and are
+ on an alternative haplotype of the same region. There are not
+ currently very many of these. This method will return a
+ reference to an empty list if no alternative alleles are found.
+ Returntype : reference to a list of genes
+ Exceptions : throw if incorrect arg provided
+ warning if gene arg does not have dbID
+ Caller : Gene::get_all_alt_alleles
=cut
-sub get_stable_entry_info {
- my ($self,$gene) = @_;
+sub fetch_all_alt_alleles {
+ my $self = shift;
+ my $gene = shift;
- if( !defined $gene || !ref $gene || !$gene->isa('Bio::EnsEMBL::Gene') ) {
- $self->throw("Needs a gene object, not a $gene");
+ if(!ref($gene) || !$gene->isa('Bio::EnsEMBL::Gene')) {
+ throw('Bio::EnsEMBL::Gene argument is required');
}
- my $sth = $self->prepare("SELECT stable_id, UNIX_TIMESTAMP(created),
- UNIX_TIMESTAMP(modified), version
- FROM gene_stable_id
- WHERE gene_id = ".$gene->dbID);
- $sth->execute();
+ my $gene_id = $gene->dbID();
- my @array = $sth->fetchrow_array();
- $gene->{'_stable_id'} = $array[0];
- $gene->{'_created'} = $array[1];
- $gene->{'_modified'} = $array[2];
- $gene->{'_version'} = $array[3];
-
+ if(!$gene_id) {
+ warning('Cannot retrieve alternate alleles for gene without dbID');
+ return [];
+ }
- return 1;
+ my $sth = $self->prepare("SELECT aa1.gene_id " .
+ "FROM alt_allele aa1, alt_allele aa2 " .
+ "WHERE aa1.alt_allele_id = aa2.alt_allele_id " .
+ "AND aa2.gene_id = ? " .
+ "AND aa1.gene_id <> ?");
+
+ $sth->execute($gene_id, $gene_id);
+
+ my @alt_ids;
+ my $row;
+ while($row = $sth->fetchrow_arrayref()) {
+ push @alt_ids, $row->[0];
+ }
+ $sth->finish();
+
+ if(@alt_ids) {
+ return $self->fetch_all_by_dbID_list(\@alt_ids);
+ }
+
+ return [];
+
}
-=head2 fetch_all_by_DBEntry
- Arg [1] : in $external_id
- the external identifier for the gene to be obtained
- Example : @genes = @{$gene_adaptor->fetch_all_by_DBEntry($ext_id)}
- Description: retrieves a list of genes with an external database
- idenitifier $external_id
- Returntype : listref of Bio::EnsEMBL::DBSQL::Gene in contig coordinates
- Exceptions : none
+=head2 store_alt_alleles
+
+ Arg [1] : reference to list of Bio::EnsEMBL::Genes $genes
+ Example : $gene_adaptor->store_alt_allele([$gene1, $gene2, $gene3]);
+ Description: This method creates a group of alternative aleles (i.e. locus)
+ from a set of genes. The genes should be genes from alternate
+ haplotypes which are similar. The genes must already be stored
+ in this database. At least 2 genes must be in the list reference
+ provided.
+ Returntype : none
+ Exceptions : throw on incorrect arguments
+ throw on sql error (e.g. duplicate unique id)
Caller : ?
=cut
-sub fetch_all_by_DBEntry {
+sub store_alt_alleles {
my $self = shift;
- my $external_id = shift;
- my @genes = ();
+ my $genes = shift;
- my $entryAdaptor = $self->db->get_DBEntryAdaptor();
+ if(!ref($genes) eq 'ARRAY') {
+ throw('List reference of Bio::EnsEMBL::Gene argument expected.');
+ }
+
+ my $num_genes = scalar(@$genes);
+
+ if($num_genes < 2) {
+ throw("At least 2 genes must be provided to construct alternate alleles.");
+ }
+
+ return if(!@$genes);
+
+ #
+ #insert the first gene seperately in order to get a unique identifier for
+ #the set of alleles
+ #
+ my $gene = $genes->[0];
+
+ if(!ref($gene) || !$gene->isa('Bio::EnsEMBL::Gene')) {
+ throw('List reference of Bio::EnsEMBL::Gene argument expected.');
+ }
+
+ my $gene_id = $gene->dbID();
+
+ if(!$gene_id) {
+ throw("Genes must have dbIDs in order to construct alternate alleles.");
+ }
+ my $sth = $self->prepare("INSERT INTO alt_allele (gene_id) VALUES (?)");
+ $sth->execute($gene->dbID());
+
+ my $alt_allele_id = $sth->{'mysql_insertid'};
+ $sth->finish();
+
+ #
+ # Insert all subsequent alt alleles using the alt_allele identifier
+ # from the first insert
+ #
+
+ $sth = $self->prepare("INSERT INTO alt_allele (alt_allele_id, gene_id) " .
+ "VALUES (?,?)");
+
+ for(my $i = 1; $i < $num_genes; $i++) {
+ my $gene = $genes->[$i];
- my @ids = $entryAdaptor->list_gene_ids_by_extids($external_id);
- foreach my $gene_id ( @ids ) {
- my $gene = $self->fetch_by_dbID( $gene_id );
- if( $gene ) {
- push( @genes, $gene );
+ if(!ref($gene) || !$gene->isa('Bio::EnsEMBL::Gene')) {
+ throw("List reference of Bio::EnsEMBL::Gene argument expected");
+ }
+
+ $gene_id = $gene->dbID();
+
+ if(!$gene_id) {
+ #This is an error but we have already inserted into the database
+ #delete the already inserted entries to restore the state of the
+ #database
+ $sth->finish();
+ $sth->prepare("DELETE FROM alt_allele WHERE alt_allele_id = ?");
+ $sth->execute($alt_allele_id);
+ $sth->finish();
+ throw('Genes must have dbIDs in order to construct alternate alleles.');
+ }
+
+ eval {
+ $sth->execute($alt_allele_id, $gene_id);
+ };
+
+ if($@) {
+ #an error occured, revert the db to the previous state
+ $sth = $self->prepare("DELETE FROM alt_allele WHERE alt_allele_id = ?");
+ $sth->execute($alt_allele_id);
+ $sth->finish();
+ throw("An SQL error occured inserting alternate alleles:\n$@");
}
}
- return \@genes;
+
+ $sth->finish();
+
+ return;
}
@@ -718,120 +581,131 @@ sub fetch_all_by_DBEntry {
=cut
sub store {
- my ($self,$gene) = @_;
- my %done;
-
- my $transcriptAdaptor = $self->db->get_TranscriptAdaptor();
-
- if( !defined $gene || !ref $gene || !$gene->isa('Bio::EnsEMBL::Gene') ) {
- $self->throw("Must store a gene object, not a $gene");
- }
- if( !defined $gene->analysis ) {
- $self->throw("Genes must have an analysis object!");
- }
+ my ( $self, $gene ) = @_;
- my $aA = $self->db->get_AnalysisAdaptor();
- my $analysisId = $aA->exists( $gene->analysis() );
+ if(!ref $gene || !$gene->isa('Bio::EnsEMBL::Gene') ) {
+ throw("Must store a gene object, not a $gene");
+ }
- if( defined $analysisId ) {
- $gene->analysis()->dbID( $analysisId );
- } else {
- $analysisId = $self->db->get_AnalysisAdaptor()->store( $gene->analysis );
- }
+ my $db = $self->db();
- if ( !defined $gene || ! $gene->isa('Bio::EnsEMBL::Gene') ) {
- $self->throw("$gene is not a EnsEMBL gene - not writing!");
- }
-
- my $trans_count = scalar(@{$gene->get_all_Transcripts});
- my $type = "";
+ if($gene->is_stored($db)) {
+ return $gene->dbID();
+ }
- if (defined($gene->type)) {
- $type = $gene->type;
- }
+ #force lazy-loading of transcripts and exons, and ensure coords are correct
+ $gene->recalculate_coordinates();
- # assuming that the store is used during the Genebuil process, set
- # the display_xref_id to 0. This ought to get re-set during the protein
- # pipeline run. This probably update to the gene table has yet to be
- # implemented.
- my $xref_id = 0;
+ my $analysis = $gene->analysis();
+ throw("Genes must have an analysis object.") if(!defined($analysis));
- my $sth2 = $self->prepare("INSERT INTO gene(type, analysis_id,
- transcript_count,
- display_xref_id)
- VALUES(?,?,?,?)");
- $sth2->execute("$type", $analysisId, $trans_count, $xref_id);
+ my $analysis_id;
+ if($analysis->is_stored($db)) {
+ $analysis_id = $analysis->dbID();
+ } else {
+ $analysis_id = $db->get_AnalysisAdaptor->store($analysis);
+ }
- my $gene_dbID = $sth2->{'mysql_insertid'};
+ my $type = $gene->type || "";
+
+ my $original = $gene;
+ my $seq_region_id;
+ ($gene, $seq_region_id) = $self->_pre_store($gene);
+
+ my $store_gene_sql =
+ "INSERT INTO gene " .
+ "SET type = ?, " .
+ "analysis_id = ?, " .
+ "seq_region_id = ?, " .
+ "seq_region_start = ?, " .
+ "seq_region_end = ?, " .
+ "seq_region_strand = ? ";
+
+ my $sth = $self->prepare( $store_gene_sql );
+ $sth->execute(
+ "$type",
+ $analysis_id,
+ $seq_region_id,
+ $gene->start(),
+ $gene->end(),
+ $gene->strand()
+ );
+ $sth->finish();
+
+ my $gene_dbID = $sth->{'mysql_insertid'};
+ #
+ # store stable ids if they are available
+ #
if (defined($gene->stable_id)) {
- if (!defined($gene->created) ||
- !defined($gene->modified) ||
- !defined($gene->version)) {
- $self->throw("Trying to store incomplete stable id information for gene");
- }
- my $statement = "INSERT INTO gene_stable_id(gene_id," .
- "version, stable_id, created, modified)".
- " VALUES(" . $gene_dbID . "," .
- $gene->version . "," .
- "'" . $gene->stable_id . "'," .
- "FROM_UNIXTIME(".$gene->created."),".
- "FROM_UNIXTIME(".$gene->modified."))";
- my $sth = $self->prepare($statement);
- $sth->execute();
- }
+ my $statement = "INSERT INTO gene_stable_id
+ SET gene_id = ?,
+ stable_id = ?,
+ version = ?";
- #
- # store the gene description associated with this gene if there is one
- #
- my $desc = $gene->description();
- if(defined($desc)) {
- my $sth = $self->prepare("INSERT INTO gene_description " .
- " SET gene_id = ?, " .
- " description = ?" );
- $sth->execute($gene_dbID, $desc);
+ $sth = $self->prepare($statement);
+ $sth->execute( $gene_dbID, $gene->stable_id(), $gene->version() );
$sth->finish();
}
+
+ #
+ # store the gene description associated with this gene if there is one
+ #
+ my $desc = $gene->description();
+ if(defined($desc)) {
+ $sth = $self->prepare("INSERT INTO gene_description " .
+ " SET gene_id = ?, " .
+ " description = ?" );
+ $sth->execute($gene_dbID, $desc);
+ $sth->finish();
+ }
+
#
# store the dbentries associated with this gene
#
- my $dbEntryAdaptor = $self->db->get_DBEntryAdaptor();
+ my $dbEntryAdaptor = $db->get_DBEntryAdaptor();
foreach my $dbe ( @{$gene->get_all_DBEntries} ) {
$dbEntryAdaptor->store( $dbe, $gene_dbID, "Gene" );
}
- #
- # Update the genes display xref if it is set
- #
- my $display_xref = $gene->display_xref;
- if($display_xref) {
- if(my $dbe_id = $dbEntryAdaptor->exists($display_xref)) {
- my $dispxref_sth = $self->prepare('UPDATE gene SET display_xref_id = ?
- WHERE gene_id = ?');
- $dispxref_sth->execute($dbe_id, $gene_dbID);
+
+ # we allow transcripts not to share equal exons and instead have copies
+ # For the database we still want sharing though, to have easier time with
+ # stable ids. So we need to have a step to merge exons together before store
+ my %exons;
+
+ foreach my $trans ( @{$gene->get_all_Transcripts} ) {
+ foreach my $e ( @{$trans->get_all_Exons} ) {
+ my $key = $e->hashkey();
+
+ if( exists $exons{ $key } ) {
+ $trans->swap_exons( $e, $exons{$key} );
+ } else {
+ $exons{$key} = $e;
+ }
}
}
-
- # write exons transcripts and exon_transcript table
- my $trans = $gene->get_all_Transcripts;
- #force lazy loading of translations before new exon dbIDs are set
- map {$_->translation} @$trans;
+ my $transcript_adaptor = $db->get_TranscriptAdaptor();
- foreach my $t ( @$trans ) {
- $transcriptAdaptor->store($t,$gene_dbID );
- }
+ foreach my $t ( @{$gene->get_all_Transcripts()} ) {
+ $transcript_adaptor->store($t,$gene_dbID );
+ }
- $gene->adaptor( $self );
- $gene->dbID( $gene_dbID );
-
- return $gene->dbID;
+ #set the adaptor and dbID on the original passed in gene not the transfered
+ #copy
+ $original->adaptor( $self );
+ $original->dbID( $gene_dbID );
+
+ return $gene_dbID;
}
+
+
=head2 remove
Arg [1] : Bio::EnsEMBL::Gene $gene
@@ -856,12 +730,16 @@ sub remove {
$sth->execute( $gene->dbID );
$sth= $self->prepare( "delete from gene_stable_id where gene_id = ? " );
$sth->execute( $gene->dbID );
+ $sth = $self->prepare( "delete from gene_description where gene_id = ?" );
+ $sth->execute( $gene->dbID() );
+
my $transcriptAdaptor = $self->db->get_TranscriptAdaptor();
foreach my $trans ( @{$gene->get_all_Transcripts()} ) {
$transcriptAdaptor->remove($trans,$gene);
}
- $gene->{'_dbID'} = undef;
+ $gene->dbID(undef);
+ $gene->adaptor(undef);
}
@@ -885,14 +763,16 @@ sub get_Interpro_by_geneid {
my $sql="
SELECT i.interpro_ac,
x.description
- FROM transcript t,
+ FROM transcript t,
+ translation tl,
protein_feature pf,
interpro i,
- xref x,
+ xref x,
gene_stable_id gsi
WHERE gsi.stable_id = '$gene'
AND t.gene_id = gsi.gene_id
- AND t.translation_id = pf.translation_id
+ AND tl.transcript_id = t.transcript_id
+ AND tl.translation_id = pf.translation_id
AND i.id = pf.hit_id
AND i.interpro_ac = x.dbprimary_acc";
@@ -915,84 +795,288 @@ sub get_Interpro_by_geneid {
-=head2 get_description
- Arg [1] : int $dbID
- the database identifier of the gene whose description should be
- obtained
- Example : $description = $gene_adaptor->get_description(1234);
- Description: Retrieves a description for a gene which is created during
- the gene build and stored in the the gene_description table.
- In the future this method should check the family database
- for a description if one does not exist in the core, however,
- this has not been implemented yet.
- Returntype : string
- Exceptions : thrown if $dbId arg is not defined
- Caller : geneview
+# deleteObj
+# Arg [1] : none
+# Example : none
+# Description: Responsible for cleaning up this objects references to other
+# objects so that proper garbage collection can occur.
+# Returntype : none
+# Exceptions : none
+# Caller : DBConnection::DeleteObj
+
+sub deleteObj {
+ my $self = shift;
+
+ #print STDERR "\t\tGeneAdaptor::deleteObj\n";
+
+ #call superclass destructor
+ $self->SUPER::deleteObj();
+
+}
+
+
+
+
+
+=head2 update
+
+ Arg [1] : Bio::EnsEMBL::Gene
+ Example : $gene_adaptor->update($gene);
+ Description: Updates the type, analysis and display_xref of a gene in the
+ database.
+ Returntype : None
+ Exceptions : thrown if the $gene is not a Bio::EnsEMBL::Gene
+ Caller : general
=cut
-sub get_description {
- my ($self, $dbID) = @_;
+sub update {
+ my ($self,$gene) = @_;
+ my $update = 0;
- if( !defined $dbID ) {
- $self->throw("must call with dbID");
+ if( !defined $gene || !ref $gene || !$gene->isa('Bio::EnsEMBL::Gene') ) {
+ throw("Must update a gene object, not a $gene");
+ }
+
+ my $update_gene_sql = "
+ UPDATE gene
+ SET type = ?,
+ analysis_id = ?,
+ display_xref_id = ?
+ WHERE gene_id = ?";
+
+ my $display_xref = $gene->display_xref();
+ my $display_xref_id;
+
+ if( defined $display_xref && $display_xref->dbID() ) {
+ $display_xref_id = $display_xref->dbID();
+ } else {
+ $display_xref_id = undef;
+ }
+
+ my $sth = $self->prepare( $update_gene_sql );
+ $sth->execute($gene->type(),
+ $gene->analysis->dbID(),
+ $display_xref_id,
+ $gene->dbID()
+ );
+
+ # maybe should update stable id or gene description ???
+}
+
+
+# _objs_from_sth
+
+# Arg [1] : StatementHandle $sth
+# Example : none
+# Description: PROTECTED implementation of abstract superclass method.
+# responsible for the creation of Genes
+# Returntype : listref of Bio::EnsEMBL::Genes in target coordinate system
+# Exceptions : none
+# Caller : internal
+
+sub _objs_from_sth {
+ my ($self, $sth, $mapper, $dest_slice) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
+
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $aa = $self->db->get_AnalysisAdaptor();
+ my $dbEntryAdaptor = $self->db()->get_DBEntryAdaptor();
+
+ my @genes;
+ my %analysis_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my ( $gene_id, $seq_region_id, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $analysis_id, $type, $display_xref_id,
+ $gene_description, $stable_id, $version, $external_name, $external_db,
+ $external_status );
+
+ $sth->bind_columns( \$gene_id, \$seq_region_id, \$seq_region_start,
+ \$seq_region_end, \$seq_region_strand, \$analysis_id, \$type,
+ \$display_xref_id, \$gene_description, \$stable_id, \$version,
+ \$external_name, \$external_db, \$external_status );
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
}
- my $sth = $self->prepare("SELECT description
- FROM gene_description
- WHERE gene_id = ?");
- $sth->execute($dbID);
- my @array = $sth->fetchrow_array();
- return $array[0];
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
+
+ FEATURE: while($sth->fetch()) {
+ #get the analysis object
+ my $analysis = $analysis_hash{$analysis_id} ||=
+ $aa->fetch_by_dbID($analysis_id);
+
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+
+ $slice = $dest_slice;
+ }
+
+ my $display_xref;
+
+ if( $display_xref_id ) {
+ $display_xref = Bio::EnsEMBL::DBEntry->new_fast
+ ({ 'dbID' => $display_xref_id,
+ 'adaptor' => $dbEntryAdaptor,
+ 'display_id' => $external_name,
+ 'dbname' => $external_db
+ });
+ $display_xref->status( $external_status );
+ }
+
+ #finally, create the new gene
+ push @genes, Bio::EnsEMBL::Gene->new
+ ( '-analysis' => $analysis,
+ '-type' => $type,
+ '-start' => $seq_region_start,
+ '-end' => $seq_region_end,
+ '-strand' => $seq_region_strand,
+ '-adaptor' => $self,
+ '-slice' => $slice,
+ '-dbID' => $gene_id,
+ '-stable_id' => $stable_id,
+ '-version' => $version,
+ '-description' => $gene_description,
+ '-external_name' => $external_name,
+ '-external_db' => $external_db,
+ '-external_status' => $external_status,
+ '-display_xref' => $display_xref );
+ }
+
+ return \@genes;
}
-=head2 deleteObj
+=head2 fetch_by_maximum_DBLink
+
+ Description: DEPRECATED - use fetch_all_by_external_name instead
- Arg [1] : none
- Example : none
- Description: Responsible for cleaning up this objects references to other
- objects so that proper garbage collection can occur.
- Returntype : none
- Exceptions : none
- Caller : DBConnection::DeleteObj
=cut
-sub deleteObj {
- my $self = shift;
-
- #print STDERR "\t\tGeneAdaptor::deleteObj\n";
+sub fetch_by_maximum_DBLink {
+ my ( $self, $external_id ) = @_;
- #call superclass destructor
- $self->SUPER::deleteObj();
-
- #flush the cache
- %{$self->{'_slice_gene_cache'}} = ();
+ my $genes=$self->fetch_all_by_DBEntry( $external_id);
+
+ my $biggest;
+ my $max=0;
+ my $size=scalar(@$genes);
+ if ($size > 0) {
+ foreach my $gene (@$genes) {
+ my $size = scalar(@{$gene->get_all_Exons});
+ if ($size > $max) {
+ $biggest = $gene;
+ $max=$size;
+ }
+ }
+ return $biggest;
+ }
+ return;
}
-
=head2 get_display_xref
- Arg [1] : Bio::EnsEMBL::Gene $gene
- retrieve display_xref for this gene
- Example : none
- Description: Retrieves the display_xref for a gene.
- Returntype : Bio::EnsEMBL::DBEntry
- Exceptions : thrown if $dbId arg is not defined
- Caller : general
+ Description: DEPRECATED use $gene->display_xref
=cut
sub get_display_xref {
my ($self, $gene ) = @_;
+ deprecate( "display xref should retrieved from Gene object directly" );
+
if( !defined $gene ) {
- $self->throw("Must call with a Gene object");
+ throw("Must call with a Gene object");
}
my $sth = $self->prepare("SELECT e.db_name,
@@ -1024,54 +1108,95 @@ sub get_display_xref {
}
+=head2 get_description
+
+ Description: DEPRECATED, use gene->get_description
+
+=cut
+
+sub get_description {
+ my ($self, $dbID) = @_;
+
+ deprecate( "Gene description should be loaded on gene retrieval. Use gene->get_description()" );
+
+ if( !defined $dbID ) {
+ throw("must call with dbID");
+ }
+
+ my $sth = $self->prepare("SELECT description
+ FROM gene_description
+ WHERE gene_id = ?");
+ $sth->execute($dbID);
+ my @array = $sth->fetchrow_array();
+ return $array[0];
+}
-=head2 update
- Arg [1] : Bio::EnsEMBL::Gene
- Example : $gene_adaptor->update($gene);
- Description: Updates a gene in the database
- Returntype : None
- Exceptions : thrown if the $gene is not a Bio::EnsEMBL::Gene
- warn if trying to update the number of attached transcripts. This
- is a far more complex process and is not yet implemented.
- warn if the method is called on a gene that does not exist in the
- database.
- Caller : general
+
+
+=head2 fetch_by_Peptide_id
+
+ Description: DEPRECATED, use fetch_by_translation_stable_id()
=cut
-sub update {
- my ($self,$gene) = @_;
- my $update = 0;
+sub fetch_by_Peptide_id {
+ my ( $self, $translation_stable_id) = @_;
- if( !defined $gene || !ref $gene || !$gene->isa('Bio::EnsEMBL::Gene') ) {
- $self->throw("Must update a gene object, not a $gene");
- }
+ deprecate( "Please use better named fetch_by_translation_stable_id" );
- my $xref_id;
- if($gene->display_xref && $gene->display_xref->dbID) {
- $xref_id = $gene->display_xref->dbID;
- } else {
- $xref_id = 0;
- }
+ $self->fetch_by_translation_stable_id($translation_stable_id);
+}
+
+
+=head2 get_stable_entry_info
+
+ Description: DEPRECATED use $gene->stable_id instead
+
+=cut
- my $tcount = scalar @{$gene->get_all_Transcripts};
+sub get_stable_entry_info {
+ my ($self,$gene) = @_;
+
+ deprecated( "stable id info is loaded on default, no lazy loading necessary" );
+
+ if( !defined $gene || !ref $gene || !$gene->isa('Bio::EnsEMBL::Gene') ) {
+ throw("Needs a gene object, not a $gene");
+ }
+
+ my $sth = $self->prepare("SELECT stable_id, UNIX_TIMESTAMP(created),
+ UNIX_TIMESTAMP(modified), version
+ FROM gene_stable_id
+ WHERE gene_id = ".$gene->dbID);
+ $sth->execute();
+
+ my @array = $sth->fetchrow_array();
+ $gene->{'stable_id'} = $array[0];
+ $gene->{'created'} = $array[1];
+ $gene->{'modified'} = $array[2];
+ $gene->{'version'} = $array[3];
+
+ return 1;
+}
- my $sth = $self->prepare("UPDATE gene
- SET type = ?,
- analysis_id = ?,
- display_xref_id = ?,
- transcript_count = ?
- WHERE gene_id = ?");
- $sth->execute($gene->type,
- $gene->analysis->dbID,
- $xref_id,
- $tcount,
- $gene->dbID);
+
+=head2 fetch_all_by_DBEntry
+
+ Description: DEPRECATED - Use fetch_all_by_external_name instead
+
+=cut
+
+sub fetch_all_by_DBEntry {
+ my $self = shift;
+ deprecate('This method has been deprecated because there was another.' .
+ "Method which did exactly the same thing.\n" .
+ 'Use fetch_all_by_external_name instead.');
+ return $self->fetch_all_by_external_name(@_);
}
+
1;
__END__
diff --git a/modules/Bio/EnsEMBL/DBSQL/KaryotypeBandAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/KaryotypeBandAdaptor.pm
index bdde897e01..4b2264d683 100644
--- a/modules/Bio/EnsEMBL/DBSQL/KaryotypeBandAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/KaryotypeBandAdaptor.pm
@@ -1,7 +1,6 @@
#
# Ensembl module for Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor
#
-# Cared for by James Stalker <jws@sanger.ac.uk>
#
# Copyright James Stalker
#
@@ -16,10 +15,17 @@ Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor
=head1 SYNOPSIS
$kary_adaptor = $db_adaptor->get_KaryotypeBandAdaptor();
-foreach $band ( $kary_adaptor->fetch_by_Slice($slice) ) {
+foreach $band ( @{$kary_adaptor->fetch_all_by_Slice($slice)} ) {
#do something with band
}
+$band = $kary_adaptor->fetch_by_dbID($id);
+
+my @bands = @{$kary_adaptor->fetch_all_by_chr_name('X')};
+
+my $band = $kary_adaptor->fetch_by_chr_band('4','q23');
+
+
=head1 DESCRIPTION
Database adaptor to provide access to KaryotypeBand objects
@@ -42,81 +48,93 @@ methods are usually preceded with a _
=cut
-# Let the code begin...
+package Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor;
+use strict;
-package Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor;
use vars qw(@ISA);
+
use Bio::EnsEMBL::KaryotypeBand;
-use strict;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning deprecate);
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
-# Object preamble - inherits from Bio::EnsEMBL::BaseAdaptor
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+#_tables
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED Implementation of abstract superclass method to
+# provide the name of the tables to query
+# Returntype : string
+# Exceptions : none
+# Caller : internal
-@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+sub _tables {
+ my $self = shift;
-# inherit new from BaseAdaptor
+ return (['karyotype','k'])
+}
-=head2 fetch_all_by_Slice
+#_columns
- Arg [1] : Bio::EnsEMBL::Slice $slice
- The slice object covering the region to retrieve bands from
- Example : @bands = @{$karyotype_band_adaptor->fetch_all_by_Slice($slice)};
- Description: Fetches karyotype band object from the database for the
- region given by the slice.
- Returntype : listref of Bio::EnsEMBL::KaryotypeBand objects in
- Slice coordinates
- Exceptions : none
- Caller : Bio::EnsEMBL::Slice::get_KarytypeBands
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED Implementation of abstract superclass method to
+# provide the name of the columns to query
+# Returntype : list of strings
+# Exceptions : none
+# Caller : internal
-=cut
+sub _columns {
+ my $self = shift;
+
+ #warning _objs_from_sth implementation depends on ordering
+ return qw (
+ k.karyotype_id
+ k.seq_region_id
+ k.seq_region_start
+ k.seq_region_end
+ k.band
+ k.stain );
+}
+
+
+sub _objs_from_sth {
+ my ($self, $sth) = @_;
+ my $db = $self->db();
+ my $slice_adaptor = $db->get_SliceAdaptor();
+
+ my @features;
+ my %slice_cache;
-sub fetch_all_by_Slice {
- my ($self,$slice) = @_;
-
- my $start = $slice->chr_start();
- my $end = $slice->chr_end();
- my $chr_name = $slice->chr_name();
-
- my $chr_adaptor = $self->db()->get_ChromosomeAdaptor();
- my $chr_id = $chr_adaptor->fetch_by_chr_name($chr_name)->dbID();
-
- my $sth = $self->prepare(" SELECT chr_start,
- chr_end,
- band,
- stain
- FROM karyotype
- WHERE chromosome_id = $chr_id
- AND $start <= chr_end
- AND $end > chr_start
- order by chr_start
- ");
-
- $sth->execute;
- my @bands = ();
- my ($chr_start,$chr_end,$band,$stain) = ();
-
- while (($chr_start,$chr_end,$band,$stain) = $sth->fetchrow_array()){
- last unless defined $band;
- my $band_obj = Bio::EnsEMBL::KaryotypeBand->new();
- $band_obj->name($band);
- $band_obj->chr_name($chr_name);
-
- #convert to slice coordinates
- $band_obj->start($chr_start - $start + 1);
- $band_obj->end($chr_end - $start + 1);
-
- $band_obj->stain($stain);
- push (@bands, $band_obj);
+ my($karyotype_id,$seq_region_id,$seq_region_start,$seq_region_end,
+ $band,$stain);
+
+ $sth->bind_columns(\$karyotype_id, \$seq_region_id, \$seq_region_start,
+ \$seq_region_end, \$band, \$stain);
+
+ while($sth->fetch()) {
+ my $slice = $slice_cache{$seq_region_id} ||=
+ $slice_adaptor->fetch_by_seq_region_id($seq_region_id);
+
+ push @features, Bio::EnsEMBL::KaryotypeBand->new
+ (-START => $seq_region_start,
+ -END => $seq_region_end,
+ -SLICE => $slice,
+ -ADAPTOR => $self,
+ -DBID => $karyotype_id,
+ -NAME => $band,
+ -STAIN => $stain);
}
- return \@bands;
+ return \@features;
}
+
=head2 fetch_all_by_chr_name
Arg [1] : string $chr_name
@@ -134,36 +152,12 @@ sub fetch_all_by_Slice {
sub fetch_all_by_chr_name {
my ($self,$chr_name) = @_;
- my $chr_adaptor = $self->db()->get_ChromosomeAdaptor();
- my $chr_id = $chr_adaptor->fetch_by_chr_name($chr_name)->dbID();
-
- my $sth = $self->prepare(
- "SELECT chr_start, chr_end, stain, band
- FROM karyotype
- WHERE chromosome_id = ?
- ORDER BY chr_start"
- );
- $sth->execute( $chr_id );
-
- my ($chr_start,$chr_end,$stain, $band) = ();
- my @out = ();
-
- while(($chr_start, $chr_end, $stain, $band) = $sth->fetchrow_array()) {
- last unless defined $chr_start;
-
- my $band_obj = Bio::EnsEMBL::KaryotypeBand->new();
- $band_obj->name($band);
- $band_obj->chr_name($chr_name);
- $band_obj->start($chr_start);
- $band_obj->end($chr_end);
- $band_obj->stain($stain);
-
- push @out, $band_obj;
- }
-
- return \@out;
-}
+ throw('Chromosome name argument expected') if(!$chr_name);
+ my $slice =
+ $self->db->get_SliceAdaptor->fetch_by_region('chromosome', $chr_name);
+ return $self->fetch_all_by_Slice($slice);
+}
=head2 fetch_by_chr_band
@@ -172,46 +166,59 @@ sub fetch_all_by_chr_name {
Name of the chromosome from which to retrieve the band
Arg [2] : string $band
The name of the band to retrieve from the specified chromosome
- Example : $band = $kary_adaptor->fetch_all_by_chr_band('4', 'q23');
- Description: Fetches the karyotype band object from the database
- for the given chromosome and band name.
- Returntype : Bio::EnsEMBL::KaryotypeBand in
- chromosomal (assembly) coordinates
+ Example : @bands = @{$kary_adaptor->fetch_all_by_chr_band('4', 'q23')};
+ Description: Fetches the karyotype band object from the database
+ for the given chromosome and band name. If no such band
+ exists, undef is returned instead. This function uses fuzzy
+ matching of the band name. For example the bands 'q23.1' and
+ 'q23.4' could be matched by fetch_all_by_chr_band('20', 'q23');
+ Returntype : Bio::EnsEMBL::KaryotypeBand in chromosomal coordinates.
Exceptions : none
Caller : general
=cut
-sub fetch_by_chr_band {
- my ($self,$chr_name, $band) = @_;
-
- $self->throw("Need band name") unless defined $band;
+sub fetch_all_by_chr_band {
+ my ($self, $chr_name, $band) = @_;
- my $chr_adaptor = $self->db()->get_ChromosomeAdaptor();
-
- my $chr_id = $chr_adaptor->fetch_by_chr_name($chr_name)->dbID();
+ throw('Chromosome name argument expected') if(!$chr_name);
+ throw('Band argument expected') if(!$band);
- my $sth = $self->prepare(
- "SELECT chr_start, chr_end, stain
- FROM karyotype
- WHERE band = ? AND chromosome_id = ?");
+ my $slice = $self->db->get_SliceAdaptor->fetch_by_region('chromosome',
+ $chr_name);
- $sth->execute( $band, $chr_id );
+ my $constraint = "k.band like '$band%'";
+ return $self->fetch_all_by_Slice_constraint($slice,$constraint);
+}
- my ($chr_start,$chr_end,$stain) = $sth->fetchrow_array;
- return undef unless defined $chr_start;
- my $band_obj = Bio::EnsEMBL::KaryotypeBand->new();
- $band_obj->name($band);
- $band_obj->chr_name($chr_name);
- $band_obj->start($chr_start);
- $band_obj->end($chr_end);
- $band_obj->stain($stain);
+sub fetch_by_chr_band {
+ my $self = shift;
+ deprecate('Use fetch_all_by_chr_band instead.');
- return $band_obj;
+ my ($band) = @{$self->fetch_all_by_chr_band(@_)};
+ return $band;
}
+=head2 list_dbIDs
+
+ Arg [1] : none
+ Example : @kary_ids = @{$karyotype_band_adaptor->list_dbIDs()};
+ Description: Gets an array of internal ids for all karyotype bands in the
+ current db
+ Returntype : reference to a list of ints
+ Exceptions : none
+ Caller : ?
+
+=cut
+
+sub list_dbIDs {
+ my $self = shift;
+
+ return $self->_list_dbIDs("karyotype");
+}
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/MetaContainer.pm b/modules/Bio/EnsEMBL/DBSQL/MetaContainer.pm
index 10d268dc0a..2779787da4 100644
--- a/modules/Bio/EnsEMBL/DBSQL/MetaContainer.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/MetaContainer.pm
@@ -24,22 +24,20 @@
=head1 CONTACT
-=head1 APPENDIX
+ Post questions to the EnsEMBL development list: ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-
-# Let the code begin...
-
package Bio::EnsEMBL::DBSQL::MetaContainer;
use vars qw(@ISA);
use strict;
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
+
use Bio::Species;
@@ -48,7 +46,6 @@ use Bio::Species;
# new() is inherited from Bio::EnsEMBL::DBSQL::BaseAdaptor
-
=head2 list_value_by_key
Arg [1] : string $key
@@ -64,7 +61,7 @@ use Bio::Species;
sub list_value_by_key {
my ($self,$key) = @_;
my @result;
-
+
$self->{'cache'} ||= {};
if( exists $self->{'cache'}->{$key} ) {
return $self->{'cache'}->{$key};
@@ -112,29 +109,33 @@ sub store_key_value {
return;
}
-=head2 update_key_value
+
+=head2 delete_key
Arg [1] : string $key
- a key under which $value should be updated
- Arg [2] : string $value
- the value to update in the meta table
- Example : $meta_container->update_key_value($key, $value);
- Description: update a value in the meta container, accessable by a key
+ The key which should be removed from the database.
+ Example : $meta_container->delete_key('sequence.compression');
+ Description: Removes all rows from the meta table which have a meta_key
+ equal to $key.
Returntype : none
Exceptions : none
- Caller : ?
+ Caller : dna_compress script, general
=cut
-sub update_key_value {
- my ( $self, $key, $value ) = @_;
+sub delete_key {
+ my ($self, $key) = @_;
- my $sth = $self->prepare( "UPDATE meta SET meta_value = ? WHERE meta_key = ?" );
+ my $sth = $self->prepare("DELETE FROM meta WHERE meta_key = ?");
+ $sth->execute($key);
+ $sth->finish();
+
+ delete $self->{'cache'}->{$key};
- my $res = $sth->execute( $value, $key );
return;
}
+
# add well known meta info get-functions below
=head2 get_Species
@@ -197,83 +198,33 @@ sub get_taxonomy_id {
}
-=head2 get_default_assembly_name
-
- Arg [1] : none
- Example : $assembly = $meta_container->get_default_assembly_name();
- Description: Retrieves the default assembly name for this database from the
- meta container
- Returntype : string
- Exceptions : none
- Caller : ?
-
-=cut
-
-sub get_default_assembly_name {
- my $self = shift;
-
- my $arrRef = $self->list_value_by_key('assembly.name' );
-
- if( @$arrRef ) {
- return $arrRef->[0];
- } else {
- return undef;
- }
-}
-
-=head2 get_default_assembly_version
-
- Arg [1] : none
- Example : $assembly = $meta_container->get_default_assembly_version();
- Description: Retrieves the default assembly version for this database from the
- meta container
- Returntype : string
- Exceptions : none
- Caller : ?
-
-=cut
-
-sub get_default_assembly_version {
- my $self = shift;
-
- my $arrRef = $self->list_value_by_key('assembly.version' );
-
- if( @$arrRef ) {
- return $arrRef->[0];
- } else {
- return undef;
- }
-}
-
=head2 get_default_assembly
- Arg [1] : none
- Example : $assembly = $meta_container->get_default_assembly();
- Description: Retrieves the default assembly for this database from the
- meta container
- Returntype : string
- Exceptions : none
- Caller : ?
+Description: DEPRECATED. Use CoordSystemAdaptor::fetch_top_level instead to
+ obtain the assembly version.
=cut
sub get_default_assembly {
my $self = shift;
- my $arrRef = $self->list_value_by_key('assembly.default' );
+ deprecate('Use CoordSystemAdaptor::fetch_top_level instead');
- if( @$arrRef ) {
- return $arrRef->[0];
- } else {
- return undef;
- }
+ my $cs = $self->db->get_CoordSystemAdaptor->fetch_top_level();
+
+ return $cs->version();
}
+#
+# TBD This method should be removed/deprecated
+#
sub get_max_assembly_contig {
my $self = shift;
+ deprecate('This method should either be fixed or removed');
+
my $value_list = $self->list_value_by_key( "assembly.maxcontig" );
if( @$value_list ) {
return $value_list->[0];
@@ -283,5 +234,6 @@ sub get_max_assembly_contig {
}
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/MiscFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/MiscFeatureAdaptor.pm
new file mode 100644
index 0000000000..432df679e9
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DBSQL/MiscFeatureAdaptor.pm
@@ -0,0 +1,474 @@
+#
+# EnsEMBL module for Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor
+#
+# Copyright EMBL/EBI
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor
+
+=head1 SYNOPSIS
+
+$mfa = $database_adaptor->get_MiscFeatureAdaptor();
+
+my $misc_feat = $mfa->fetch_by_dbID(1234);
+my @misc_feats = @{$mfa->fetch_all_by_Slice($slice)};
+
+my @misc_clones = @{$mfa->fetch_all_by_Slice_and_set_code('cloneset')};
+
+
+=head1 DESCRIPTION
+
+This is an adaptor for the retrieval and storage of MiscFeatures. Misc
+Features are extremely generic features that can be added with minimal
+effort to the database. Currently misc features are used to describe
+the locations of clone sets and tiling path information, but arbitrary
+features can be stored. Misc features are grouped into sets and can be
+fetched according to their grouping using the fetch_all_by_Slice_and_set_code
+and fetch_all_by_set_code methods. MiscFeatures may belong to more
+than one set.
+
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL developer list: ensembl-dev@ebi.ac.uk
+
+=head1 APPENDIX
+
+The rest of the documentation details each of the object methods.
+Internal methods are usually preceded with a _
+
+=cut
+
+package Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor;
+
+use strict;
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
+use Bio::EnsEMBL::MiscFeature;
+use Bio::EnsEMBL::MiscSet;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
+
+
+
+=head2 fetch_all_by_Slice_and_set_code
+
+ Arg [1] : Bio::EnsEMBL::Slice $slice
+ A slice representing the region to fetch from
+ Arg [2] : string $set_code
+ The code of the set to retrieve features from
+ Example : @feats = @{$mfa->fetch_all_by_Slice_and_set_code('cloneset')};
+ Description: Retrieves a set of MiscFeatures which have a particular set code
+ and which lie in a particular region. All features with the
+ provide set code and which overlap the given slice are returned.
+ Returntype : listref of Bio::EnsEMBL::MiscFeatures
+ Exceptions : throw if set_code is not provided
+ warning if no set for provided set code exists
+ Caller : general
+
+=cut
+
+sub fetch_all_by_Slice_and_set_code {
+ my $self = shift;
+ my $slice = shift;
+ my $set_code = shift;
+
+ throw('Set code argument is required.') if(!$set_code);
+
+ my $msa = $self->db->get_MiscSetAdaptor();
+ my $set = $msa->fetch_by_code($set_code);
+
+ if(!$set) {
+ warning("No misc_set with code [$set_code] exists.\n" .
+ "Returning empty list.");
+ return [];
+ }
+
+ my $constraint = " mfms.misc_set_id = " . $set->dbID();
+ return $self->fetch_all_by_Slice_constraint($slice, $constraint);
+}
+
+
+
+=head2 fetch_all_by_attribute_type_value
+
+ Arg [1] : string $attrib_type_code
+ The code of the attribute type to fetch features for
+ Arg [2] : (optional) string $attrib_value
+ The value of the attribute to fetch features for
+ Example :
+ #get all misc features that have an embl accession
+ @feats = @{$mfa->fetch_all_by_attrib_type_value('embl_acc')};
+ #get the misc feature with synonym 'AL014121'
+ ($feat)=@{$mfa->fetch_all_by_attrib_type_value('synonym','AL014121');
+ Description: Retrieves MiscFeatures which have a particular attribute.
+ If the attribute value argument is also provided only features
+ which have the attribute AND a particular value are returned.
+ The features are returned in their native coordinate system
+ (i.e. the coordinate system that they are stored in).
+ Returntype : listref of Bio::EnsEMBL::MiscFeatures
+ Exceptions : throw if attrib_type code arg is not provided
+ Caller : general
+
+=cut
+
+sub fetch_all_by_attribute_type_value {
+ my $self = shift;
+ my $attrib_type_code = shift;
+ my $attrib_value = shift;
+
+ throw("Attrib type code argument is required.") if(!$attrib_type_code);
+
+ #
+ # Need to do 2 queries so that all of the ids come back with the features.
+ # The problem with adding attrib constraints to filter the misc_features
+ # which come back is that not all of the attributes will come back
+ #
+
+ my $sql = "SELECT DISTINCT ma.misc_feature_id " .
+ "FROM misc_attrib ma, attrib_type at " .
+ "WHERE ma.attrib_type_id = at.attrib_type_id " .
+ "AND at.code = ?";
+
+ my @bind_vals = ($attrib_type_code);
+
+ if($attrib_value) {
+ push @bind_vals, $attrib_value;
+ $sql .= " AND ma.value = ?";
+ }
+
+ my $sth = $self->prepare($sql);
+ $sth->execute(@bind_vals);
+
+ my @ids = map {$_->[0]} @{$sth->fetchall_arrayref()};
+
+ $sth->finish();
+
+ #construct constraints from the list of ids. Split ids into
+ #groups of 1000 to ensure that the query is not too big
+ my @constraints;
+ while(@ids) {
+ my @subset = splice(@ids, 0, 1000);
+ if(@subset == 1) {
+ push @constraints, "mf.misc_feature_id = $subset[0]";
+ } else {
+ my $id_str = join(',',@subset);
+ push @constraints, "mf.misc_feature_id in ($id_str)";
+ }
+ }
+
+ my @results;
+ foreach my $constraint (@constraints) {
+ push @results, @{$self->generic_fetch($constraint)};
+ }
+
+ return \@results;
+}
+
+
+#_tables
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED Implementation of abstract superclass method to
+# provide the name of the tables to query
+# Returntype : string
+# Exceptions : none
+# Caller : internal
+
+
+sub _tables {
+ my $self = shift;
+
+ return (['misc_feature', 'mf'],
+ ['misc_feature_misc_set', 'mfms'],
+ ['misc_attrib', 'ma'],
+ ['attrib_type', 'at']);
+}
+
+
+#_columns
+
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED Implementation of abstract superclass method to
+# provide the name of the columns to query
+# Returntype : list of strings
+# Exceptions : none
+# Caller : internal
+
+sub _columns {
+ my $self = shift;
+
+ #warning _objs_from_sth implementation depends on ordering
+ return qw (mf.misc_feature_id
+ mf.seq_region_id
+ mf.seq_region_start
+ mf.seq_region_end
+ mf.seq_region_strand
+ ma.value
+ at.code
+ mfms.misc_set_id);
+}
+
+
+
+# _default_where_clause
+
+# Arg [1] : none
+# Example : none
+# Description: Overrides superclass method to provide an additional
+# table joining constraint before the SQL query is performed.
+# Returntype : string
+# Exceptions : none
+# Caller : generic_fetch
+
+sub _default_where_clause {
+ my $self = shift;
+
+ return '';
+}
+
+
+sub _left_join {
+ my $self = shift;
+
+ return(
+ ['misc_feature_misc_set','mf.misc_feature_id = mfms.misc_feature_id'],
+ ['misc_attrib', 'mf.misc_feature_id = ma.misc_feature_id'],
+ ['attrib_type','ma.attrib_type_id = at.attrib_type_id']);
+}
+
+
+sub _final_clause {
+ my $self = shift;
+
+ return "order by mf.misc_feature_id";
+}
+
+
+# _objs_from_sth
+
+# Arg [1] : StatementHandle $sth
+# Example : none
+# Description: PROTECTED implementation of abstract superclass method.
+# responsible for the creation of MiscFeatures from a
+# hashref generated from an SQL query
+# Returntype : listref of Bio::EnsEMBL::MiscFeatures
+# Exceptions : none
+# Caller : internal
+
+sub _objs_from_sth {
+ my ($self, $sth, $mapper, $dest_slice) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
+
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $msa = $self->db->get_MiscSetAdaptor();
+
+ my @features;
+ my %ms_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my($misc_feature_id, $seq_region_id, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $attrib_value, $attrib_type_code, $misc_set_id);
+
+ $sth->bind_columns( \$misc_feature_id, \$seq_region_id, \$seq_region_start,
+ \$seq_region_end, \$seq_region_strand,
+ \$attrib_value, \$attrib_type_code,\$misc_set_id);
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
+
+ my $current = -1;
+ my $throw_away = -1;
+ my $feat;
+ my $feat_misc_sets;
+ my $feat_attribs;
+ my $seen_attribs;
+
+ FEATURE: while($sth->fetch()) {
+ #if this feature is not being used, skip all rows related to it
+ next if($throw_away == $misc_feature_id);
+
+ if($current == $misc_feature_id) {
+ #still working on building up attributes and sets for current feature
+
+ #if there is a misc_set, add it to the current feature
+ if($misc_set_id) {
+ my $misc_set = $ms_hash{$misc_set_id} ||=
+ $msa->fetch_by_dbID($misc_set_id);
+ #doesn't matter if it is added twice in same slot
+ $feat_misc_sets->{$misc_set->{'code'}} = $misc_set;
+ }
+
+ #if there is a new attribute add it to the current feature
+ if($attrib_value && $attrib_type_code &&
+ !$seen_attribs->{"$attrib_type_code:$attrib_value"}) {
+ $feat_attribs->{$attrib_type_code} ||= [];
+ push @{$feat_attribs->{$attrib_type_code}}, $attrib_value;
+ $seen_attribs->{"$attrib_type_code:$attrib_value"} = 1;
+ }
+
+ } else {
+ if($feat) {
+ #start working on a new feature, discard references to last one
+ $feat = {};
+ $feat_attribs = {};
+ $feat_misc_sets = {};
+ $seen_attribs = {};
+ }
+
+ $current = $misc_feature_id;
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs ||
+ ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ #flag this feature as one to throw away
+ $throw_away = $misc_feature_id;
+ next FEATURE;
+ }
+ }
+ $slice = $dest_slice;
+ }
+
+ if($misc_set_id) {
+ #get the misc_set object
+ my $misc_set = $ms_hash{$misc_set_id} ||=
+ $msa->fetch_by_dbID($misc_set_id);
+ $feat_misc_sets->{$misc_set->{'code'}} = $misc_set;
+ }
+
+ if($attrib_value && $attrib_type_code) {
+ $feat_attribs->{$attrib_type_code} = [$attrib_value];
+ $seen_attribs->{"$attrib_type_code:$attrib_value"} = 1;
+ }
+
+ $feat = Bio::EnsEMBL::MiscFeature->new_fast
+ ({'start' => $seq_region_start,
+ 'end' => $seq_region_end,
+ 'strand' => $seq_region_strand,
+ 'slice' => $slice,
+ 'adaptor' => $self,
+ 'dbID' => $misc_feature_id,
+ 'attributes' => $feat_attribs,
+ 'sets' => $feat_misc_sets});
+ push @features, $feat;
+ }
+ }
+
+ return \@features;
+}
+
+
+
+=head2 list_dbIDs
+
+ Arg [1] : none
+ Example : @feature_ids = @{$misc_feature_adaptor->list_dbIDs()};
+ Description: Gets an array of internal ids for all misc_features in the current db
+ Returntype : list of ints
+ Exceptions : none
+ Caller : ?
+
+=cut
+
+sub list_dbIDs {
+ my ($self) = @_;
+
+ return $self->_list_dbIDs("misc_feature");
+}
+
+1;
+
+
+
+
+
diff --git a/modules/Bio/EnsEMBL/DBSQL/MiscSetAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/MiscSetAdaptor.pm
new file mode 100644
index 0000000000..10c0dadec0
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DBSQL/MiscSetAdaptor.pm
@@ -0,0 +1,182 @@
+#
+# Ensembl module for Bio::EnsEMBL::DBSQL::MiscSetAdaptor
+#
+# Copyright (c) 2003 EnsEMBL
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::DBSQL::MiscSetAdaptor - Provides database interaction for
+Bio::EnsEMBL::MiscSet objects.
+
+
+=head1 SYNOPSIS
+
+ #$db is a Bio::EnsEMBL::DBSQL::DBAdaptor object:
+ $msa = $db->get_MiscSetAdaptor();
+
+ $misc_set = $msa->fetch_by_dbID(1234);
+
+ $misc_set = $msa->fetch_by_code('clone');
+
+
+=head1 DESCRIPTION
+
+This class provides database interactivity for MiscSet objects. MiscSets
+are used to classify MiscFeatures into groups.
+
+=head1 CONTACT
+
+This modules is part of the Ensembl project http://www.ensembl.org
+
+Questions can be posted to the ensembl-dev mailing list:
+ensembl-dev@ebi.ac.uk
+
+=cut
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::DBSQL::MiscSetAdaptor;
+
+use Bio::EnsEMBL::MiscSet;
+use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+
+
+=head2 new
+
+ Arg [...] : Superclass args. See Bio::EnsEMBL::DBSQL::BaseAdaptor
+ Description: Instantiates a Bio::EnsEMBL::DBSQL::MiscSetAdaptor and
+ caches the contents of the MiscSet table.
+ Returntype : Bio::EnsEMBL::MiscSet
+ Exceptions : none
+ Caller : MiscFeatureAdaptor
+
+=cut
+
+
+sub new {
+ my $class = shift;
+
+ my $self = $class->SUPER::new(@_);
+
+ #cache the entire contents of the misc set table
+ #the table is small and it removes the need to repeatedly query the
+ #table or join to the table
+
+ $self->{'_id_cache'} = {};
+ $self->{'_code_cache'} = {};
+
+ my $sth = $self->prepare
+ ('SELECT misc_set_id, code, name, description, max_length FROM misc_set');
+
+ $sth->execute();
+
+ my ($dbID, $code, $name, $desc, $max_len);
+ $sth->bind_columns(\$dbID, \$code, \$name, \$desc, \$max_len);
+
+ while($sth->fetch()) {
+ my $ms =
+ Bio::EnsEMBL::MiscSet->new($dbID, $self, $code, $name, $desc, $max_len);
+
+ $self->{'_id_cache'}->{$dbID} = $ms;
+ $self->{'_code_cache'}->{lc($code)} = $ms;
+ }
+
+ $sth->finish();
+
+ return $self;
+}
+
+
+
+
+=head2 fetch_all
+
+ Arg [1] : none
+ Example : foreach my $ms (@{$msa->fetch_all()}) {
+ print $ms->code(), ' ', $ms->name(), "\n";
+ }
+ Description: Retrieves every MapSet defined in the DB
+ Returntype : listref of Bio::EnsEMBL::MapSets
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub fetch_all {
+ my $self = shift;
+
+ my @all = values(%{$self->{'_id_cache'}});
+
+ return \@all;
+}
+
+
+
+=head2 fetch_by_dbID
+
+ Arg [1] : int $dbID
+ The internal identifier of the misc set to retrieve
+ Example : my $ms = $msa->fetch_by_dbID($dbID);
+ Description: Retrieves a misc set via its internal identifier
+ Returntype : Bio::EnsEMBL::MiscSet
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub fetch_by_dbID {
+ my $self = shift;
+ my $dbID = shift;
+
+ return $self->{'_id_cache'}->{$dbID};
+}
+
+
+
+=head2 fetch_by_code
+
+ Arg [1] : string $code
+ The unique code of the MiscSet to retrieve
+ Example : my $ms = $msa->fetch_by_code('clone');
+ Description: Retrieves a MiscSet via its code
+ Returntype : Bio::EnsEMBL::MiscSet
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub fetch_by_code {
+ my $self = shift;
+ my $code = shift;
+
+ return $self->{'_code_cache'}->{lc($code)};
+}
+
+
+
+#
+# Called during db destruction to clean up internal cache structures
+# that result in circular references
+#
+sub deleteObj {
+ my $self = shift;
+
+ #break circular db <-> adaptor references
+ $self->SUPER::deleteObj();
+
+ #break circular object <-> adaptor references
+ delete $self->{'_id_cache'};
+ delete $self->{'_code_cache'};
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/PredictionExonAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/PredictionExonAdaptor.pm
new file mode 100644
index 0000000000..4d76523f0b
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DBSQL/PredictionExonAdaptor.pm
@@ -0,0 +1,409 @@
+#EnsEMBL PredictionExon reading writing adaptor for mySQL
+#
+# Copyright EMBL-EBI 2003
+#
+# Author: Arne Stabenau
+#
+#
+
+=head1 NAME
+
+Bio::EnsEMBL::DBSQL::PredictionExonAdaptor - Performs database interaction for
+PredictionExons.
+
+=head1 SYNOPSIS
+
+$pea = $database_adaptor->get_PredictionExonAdaptor();
+$pexon = $pea->fetch_by_dbID();
+
+my $slice = $database_adaptor->get_SliceAdaptor->fetch_by_region('X',1,1e6);
+
+my @pexons = @{$pea->fetch_all_by_Slice($slice)};
+
+
+=head1 CONTACT
+
+ Post questions to the EnsEMBL development list ensembl-dev@ebi.ac.uk
+
+=head1 APPENDIX
+
+ The rest of the documentation describes object methods.
+
+=cut
+
+
+package Bio::EnsEMBL::DBSQL::PredictionExonAdaptor;
+
+use vars qw( @ISA );
+use strict;
+
+
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
+use Bio::EnsEMBL::PredictionExon;
+use Bio::EnsEMBL::Utils::Exception qw( warning throw deprecate );
+
+@ISA = qw( Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor );
+
+
+#_tables
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns the names, aliases of the tables to use for queries
+# Returntype : list of listrefs of strings
+# Exceptions : none
+# Caller : internal
+#
+
+sub _tables {
+ return ([ 'prediction_exon', 'pe' ] );
+}
+
+
+
+#_columns
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns a list of columns to use for queries
+# Returntype : list of strings
+# Exceptions : none
+# Caller : internal
+
+sub _columns {
+ my $self = shift;
+
+ return qw( pe.prediction_exon_id
+ pe.seq_region_id
+ pe.seq_region_start
+ pe.seq_region_end
+ pe.seq_region_strand
+ pe.start_phase
+ pe.score
+ pe.p_value );
+}
+
+
+# _final_clause
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns a default end for the SQL-query (ORDER BY)
+# Returntype : string
+# Exceptions : none
+# Caller : internal
+
+sub _final_clause {
+ return "ORDER BY pe.prediction_transcript_id, pe.exon_rank";
+}
+
+
+=head2 fetch_all_by_PredictionTranscript
+
+ Arg [1] : Bio::EnsEMBL::PredcitionTranscript $transcript
+ Example : none
+ Description: Retrieves all Exons for the Transcript in 5-3 order
+ Returntype : listref Bio::EnsEMBL::Exon on Transcript slice
+ Exceptions : none
+ Caller : Transcript->get_all_Exons()
+
+=cut
+
+sub fetch_all_by_PredictionTranscript {
+ my ( $self, $transcript ) = @_;
+ my $constraint = "pe.prediction_transcript_id = ".$transcript->dbID();
+
+ my $keep_all = 1; #keep transcripts that are off end of slice
+ return $self->generic_fetch( $constraint, undef,
+ $transcript->slice(), $keep_all);
+}
+
+
+
+=head2 store
+
+ Arg [1] : Bio::EnsEMBL::PredictionExon $exon
+ The exon to store in this database
+ Arg [2] : int $prediction_transcript_id
+ The internal identifier of the prediction exon that that this
+ exon is associated with.
+ Arg [3] : int $rank
+ The rank of the exon in the transcript (starting at 1)
+ Example : $pexon_adaptor->store($pexon, 1211, 2);
+ Description: Stores a PredictionExon in the database
+ Returntype : none
+ Exceptions : thrown if exon does not have a slice attached
+ or if $exon->start, $exon->end, $exon->strand, or $exon->phase
+ are not defined or if $exon is not a Bio::EnsEMBL::PredictionExon
+ Caller : general
+
+=cut
+
+sub store {
+ my ( $self, $pexon, $pt_id, $rank ) = @_;
+
+ if(!ref($pexon) || !$pexon->isa('Bio::EnsEMBL::PredictionExon') ) {
+ throw("Expected PredictionExon argument");
+ }
+
+ throw("Expected PredictionTranscript id argument.") if(!$pt_id);
+ throw("Expected rank argument.") if(!$rank);
+
+ my $db = $self->db();
+
+ if($pexon->is_stored($db)) {
+ warning('PredictionExon is already stored in this DB.');
+ return $pexon->dbID();
+ }
+
+ if( ! $pexon->start || ! $pexon->end ||
+ ! $pexon->strand || ! defined $pexon->phase ) {
+ throw("PredictionExon does not have all attributes to store.\n" .
+ "start, end, strand and phase attributes must be set.");
+ }
+
+ #maintain reference to original passed-in prediction exon
+ my $original = $pexon;
+ my $seq_region_id;
+ ($pexon, $seq_region_id) = $self->_pre_store($pexon);
+
+ my $sth = $db->prepare
+ ("INSERT into prediction_exon (prediction_transcript_id, exon_rank, " .
+ "seq_region_id, seq_region_start, seq_region_end, " .
+ "seq_region_strand, start_phase, score, p_value) " .
+ "VALUES ( ?, ?, ?, ?, ?, ?, ?, ?, ? )");
+
+ $sth->execute( $pt_id,
+ $rank,
+ $seq_region_id,
+ $pexon->start(),
+ $pexon->end(),
+ $pexon->strand(),
+ $pexon->phase(),
+ $pexon->score(),
+ $pexon->p_value());
+
+ my $dbID = $sth->{'mysql_insertid'};
+
+ #set the adaptor and dbID of the object they passed in
+ $original->dbID($dbID);
+ $original->adaptor($self);
+
+ return $dbID;
+}
+
+
+
+=head2 remove
+
+ Arg [1] : Bio::EnsEMBL::PredictionExon $exon
+ the exon to remove from the database
+ Example : $exon_adaptor->remove($exon);
+ Description: Removes an exon from the database
+ Returntype : none
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub remove {
+ my $self = shift;
+ my $pexon = shift;
+
+ my $db = $self->db();
+
+ if(!$pexon->is_stored($db)) {
+ warning('PredictionExon is not in this DB - not removing');
+ return undef;
+ }
+
+ my $sth = $self->prepare( "delete from exon where exon_id = ?" );
+ $sth->execute( $pexon->dbID );
+
+ $pexon->dbID(undef);
+ $pexon->adaptor(undef);
+}
+
+
+
+=head2 list_dbIDs
+
+ Arg [1] : none
+ Example : @exon_ids = @{$exon_adaptor->list_dbIDs()};
+ Description: Gets an array of internal ids for all exons in the current db
+ Returntype : list of ints
+ Exceptions : none
+ Caller : ?
+
+=cut
+
+sub list_dbIDs {
+ my ($self) = @_;
+
+ return $self->_list_dbIDs("prediction_exon");
+}
+
+
+
+#_objs_from_sth
+
+# Arg [1] : Hashreference $hashref
+# Example : none
+# Description: PROTECTED implementation of abstract superclass method.
+# responsible for the creation of Genes
+# Returntype : listref of Bio::EnsEMBL::Genes in target coordinate system
+# Exceptions : none
+# Caller : internal
+#
+
+sub _objs_from_sth {
+ my ($self, $sth, $mapper, $dest_slice, $keep_all) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
+ my $sa = $self->db()->get_SliceAdaptor();
+
+ my @exons;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my($prediction_exon_id,$seq_region_id,
+ $seq_region_start, $seq_region_end, $seq_region_strand,
+ $start_phase, $score, $p_value);
+
+ $sth->bind_columns(\$prediction_exon_id,\$seq_region_id,
+ \$seq_region_start, \$seq_region_end, \$seq_region_strand,
+ \$start_phase, \$score, \$p_value);
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ my $dest_slice_cs;
+ my $asma;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ $dest_slice_cs = $dest_slice->coord_system;
+ $asma = $self->db->get_AssemblyMapperAdaptor();
+ }
+
+ FEATURE: while($sth->fetch()) {
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+ my $dest_mapper = $mapper;
+
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
+
+ #obtain a mapper if none was defined, but a dest_seq_region was
+ if(!$dest_mapper && $dest_slice &&
+ !$dest_slice_cs->equals($slice->coord_system)) {
+ $dest_mapper = $asma->fetch_by_CoordSystems($dest_slice_cs,
+ $slice->coord_system);
+ $asm_cs = $dest_mapper->assembled_CoordSystem();
+ $cmp_cs = $dest_mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($dest_mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $dest_mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE if(!$keep_all);
+ }
+ }
+
+ $slice = $dest_slice;
+ }
+
+ #finally, create the new PredictionExon
+ push @exons, Bio::EnsEMBL::PredictionExon->new
+ ( '-start' => $seq_region_start,
+ '-end' => $seq_region_end,
+ '-strand' => $seq_region_strand,
+ '-adaptor' => $self,
+ '-slice' => $slice,
+ '-dbID' => $prediction_exon_id,
+ '-phase' => $start_phase,
+ '-score' => $score,
+ '-p_value' => $p_value);
+ }
+
+ return \@exons;
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/PredictionTranscriptAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/PredictionTranscriptAdaptor.pm
index 0275658ebb..e6be382e46 100644
--- a/modules/Bio/EnsEMBL/DBSQL/PredictionTranscriptAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/PredictionTranscriptAdaptor.pm
@@ -7,8 +7,8 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::PredictionTranscriptAdaptor -
-MySQL Database queries to load and store PredictionExons
+Bio::EnsEMBL::DBSQL::PredictionTranscriptAdaptor -
+Performs database interaction related to PredictionTranscripts
=head1 SYNOPSIS
@@ -17,7 +17,7 @@ $pta = $database_adaptor->get_PredictionTranscriptAdaptor();
#get a slice on a region of chromosome 1
$sa = $database_adaptor->get_SliceAdaptor();
-$slice = $sa->fetch_by_chr_start_end('1', 100000, 200000);
+$slice = $sa->fetch_by_region('x', 100000, 200000);
#get all the prediction transcripts from the slice region
$prediction_transcripts = @{$pta->fetch_all_by_Slice($slice)};
@@ -34,90 +34,63 @@ use vars qw( @ISA );
use strict;
use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
-use Bio::EnsEMBL::DBSQL::DBAdaptor;
use Bio::EnsEMBL::DBSQL::AnalysisAdaptor;
use Bio::EnsEMBL::PredictionTranscript;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate throw warning);
@ISA = qw( Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor );
-=head2 _tables
-
- Arg [1] : none
- Example : none
- Description: Implements abstract superclass method to define the table used
- to retrieve prediction transcripts from the database
- Returntype : string
- Exceptions : none
- Caller : generic_fetch
-
-=cut
+# _tables
+#
+# Arg [1] : none
+# Example : none
+# Description: Implements abstract superclass method to define the table used
+# to retrieve prediction transcripts from the database
+# Returntype : string
+# Exceptions : none
+# Caller : generic_fetch
sub _tables {
my $self = shift;
- return ['prediction_transcript', 'p'];
+ return ['prediction_transcript', 'pt'];
}
+# _columns
-=head2 _columns
-
- Arg [1] : none
- Example : none
- Description: Implements abstract superclass method to define the columns
- retrieved in database queries used to create prediction
- transcripts.
- Returntype : list of strings
- Exceptions : none
- Caller : generic_fetch
-
-=cut
+# Arg [1] : none
+# Example : none
+# Description: Implements abstract superclass method to define the columns
+# retrieved in database queries used to create prediction
+# transcripts.
+# Returntype : list of strings
+# Exceptions : none
+# Caller : generic_fetch
+#
sub _columns {
my $self = shift;
- return qw( p.prediction_transcript_id
- p.contig_id
- p.contig_start
- p.contig_end
- p.contig_strand
- p.start_phase
- p.exon_rank
- p.score
- p.p_value
- p.analysis_id
- p.exon_count);
+ return qw( pt.prediction_transcript_id
+ pt.seq_region_id
+ pt.seq_region_start
+ pt.seq_region_end
+ pt.seq_region_strand
+ pt.analysis_id);
}
-
-=head2 _final_clause
-
- Arg [1] : none
- Example : none
- Description: Overrides superclass method to provide an additional table
- joining coinstraint before the SQL query is performed.
- Returntype : string
- Exceptions : none
- Caller : generic_fetch
-
-=cut
-
-sub _final_clause {
- my $self = shift;
-
- return 'order by p.prediction_transcript_id, p.exon_rank';
-}
-
=head2 fetch_by_stable_id
Arg [1] : string $stable_id
The stable id of the transcript to retrieve
-Example : $trans = $trans_adptr->fetch_by_stable_id('3.10.190');
+Example : $trans = $trans_adptr->fetch_by_stable_id('GENSCAN00000001234');
Description: Retrieves a prediction transcript via its stable id. Note that
- the stable id is not actually stored in the database and is
- calculated upon retrieval as the contig.start.end of the
+ the stable id is not actually stored in the database and is
+ calculated upon retrieval as the logic_name of the transcripts
+ analysis concatted with the '0'-left-padded internal identifier.
prediction transcript
Returntype : Bio::EnsEMBL::PredictionTranscript
Caller : general
@@ -126,31 +99,17 @@ Caller : general
sub fetch_by_stable_id {
my $self = shift;
- my $id = shift;
+ my $stable_id = shift;
- $self->throw('ID argument is required') if(!$id);
+ throw('Stable_id argument expected') if(!$stable_id);
- #strip the start/end off the end of the id to get the contig name
- my @components = split(/\./, $id);
- pop(@components);
- pop(@components);
+ #take the last 11 digits
+ my $dbID = substr($stable_id, -11);
- my $contig_name = join('.', @components);
- my $contig = $self->db->get_RawContigAdaptor->fetch_by_name($contig_name);
+ #cast to an integer (remove lpadded 0s):
+ $dbID += 0;
- $self->throw("unknown contig [$contig_name] in identifier [$id]")
- if(!$contig);
-
- #
- # Retrieve all the prediction transcripts on the contig it is known to be on.
- # Return the one found with the matching 'id'
- #
- foreach my $pt (@{$self->fetch_all_by_RawContig($contig)}) {
- return $pt if($id eq $pt->stable_id);
- }
-
- #prediction transcript does not exist
- $self->throw("No prediction transcipt exists with stable id [$id]");
+ return $self->fetch_by_dbID($dbID);
}
@@ -178,176 +137,142 @@ sub fetch_by_stable_id {
=cut
sub _objs_from_sth {
- my ($self, $sth, $mapper, $slice) = @_;
-
- my @out = ();
-
- my ($prediction_transcript_id,
- $contig_id, $contig_start, $contig_end, $contig_strand,
- $start_phase, $exon_rank, $score, $p_value, $analysis_id,
- $exon_count );
-
- $sth->bind_columns(\$prediction_transcript_id,
- \$contig_id, \$contig_start, \$contig_end, \$contig_strand,
- \$start_phase, \$exon_rank, \$score, \$p_value,
- \$analysis_id,\$exon_count);
-
- my $rca = $self->db->get_RawContigAdaptor;
- my $aa = $self->db->get_AnalysisAdaptor;
-
- my ($analysis, $contig, $pre_trans, $ptid, $on_slice_flag, $last_end,
- $chr, $start, $end, $strand,
- $slice_start, $slice_end, $slice_strand,
- $exon, $exon_start, $exon_end, $exon_strand,
- $stable_start, $stable_end, $stable_ctg,
- $transcript_slice_start, $transcript_slice_end );
- my (%analysis_hash, %contig_hash);
-
- if($slice) {
- $slice_start = $slice->chr_start;
- $slice_end = $slice->chr_end;
- $slice_strand = $slice->strand;
+ my ($self, $sth, $mapper, $dest_slice) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
+
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $aa = $self->db()->get_AnalysisAdaptor();
+
+ my @ptranscripts;
+ my %analysis_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my ($prediction_transcript_id,
+ $seq_region_id,
+ $seq_region_start,
+ $seq_region_end,
+ $seq_region_strand,
+ $analysis_id );
+
+ $sth->bind_columns(\$prediction_transcript_id,
+ \$seq_region_id,
+ \$seq_region_start,
+ \$seq_region_end,
+ \$seq_region_strand,
+ \$analysis_id );
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
}
- $on_slice_flag = 0;
-
- my $prev_exon;
- my $already_merged;
-
- while($sth->fetch) {
- #create a new transcript for each new prediction transcript id
- unless(defined $pre_trans && $ptid == $prediction_transcript_id) {
- $pre_trans = Bio::EnsEMBL::PredictionTranscript->new;
-
- $ptid = $prediction_transcript_id;
- $pre_trans->dbID($ptid);
-
- unless($analysis = $analysis_hash{$analysis_id}) {
- $analysis = $aa->fetch_by_dbID($analysis_id);
- $analysis_hash{$analysis_id} = $analysis;
- }
-
- $pre_trans->analysis($analysis);
- $pre_trans->set_exon_count($exon_count);
-
- $prev_exon = undef;
- $already_merged = 0;
-
- if(@out) {
- #throw away last pt if no exons or introns were on the slice
- if($slice && ( $transcript_slice_end < 1 ||
- $transcript_slice_start > $slice->length() )) {
- pop @out;
- } else {
- #set the stable_id of the previous prediction
- $out[$#out]->stable_id("$stable_ctg.$stable_start.$stable_end");
- }
- }
-
- push( @out, $pre_trans );
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
- #reset values used for last predtrans
- $stable_start = -1;
- $stable_end = -1;
- $stable_ctg = '';
+ FEATURE: while($sth->fetch()) {
- $transcript_slice_end = undef;
- $transcript_slice_start = undef;
- }
+ #get the analysis object
+ my $analysis = $analysis_hash{$analysis_id} ||=
+ $aa->fetch_by_dbID($analysis_id);
- #recalculate stable id values
- if($stable_start == -1 || $contig_start < $stable_start) {
- $stable_start = $contig_start;
- }
- if($contig_end > $stable_end) {
- $stable_end = $contig_end;
- }
- unless($contig = $contig_hash{$contig_id}) {
- $contig = $rca->fetch_by_dbID($contig_id);
- $contig_hash{$contig_id} = $contig;
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
}
- $stable_ctg = $contig->name;
-
- if($slice) {
- #a slice was passed in so we want slice coords
-
- #convert contig coords to assembly coords
- ($chr, $start, $end, $strand) =
- $mapper->fast_to_assembly($contig_id, $contig_start,
- $contig_end, $contig_strand);
-
- #if mapped to gap skip
- next unless(defined $start);
-
-
- #convert to slice coordinates
- if($slice_strand == -1) {
- $exon_start = $slice_end - $end + 1;
- $exon_end = $slice_end - $start + 1;
- $exon_strand = $strand * -1;
-
- #merge adjacent exons into a single exon
- if($prev_exon && $prev_exon->start == $exon->end + 1) {
- $exon->end($prev_exon->end);
- $already_merged++;
- }
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
} else {
- $exon_start = $start - $slice_start + 1;
- $exon_end = $end - $slice_start + 1;
- $exon_strand = $strand;
-
- #merge adjacent exons into a single exon
- if($prev_exon && $exon->start == $prev_exon->end +1) {
- $exon->start($prev_exon->start);
- $already_merged++;
- }
- }
-
- if( !defined $transcript_slice_start ||
- $transcript_slice_start > $exon_start ) {
- $transcript_slice_start = $exon_start;
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
}
-
- if( ! defined $transcript_slice_end ||
- $transcript_slice_end < $exon_end ) {
- $transcript_slice_end = $exon_end;
- }
- #use slice as the contig instead of the raw contig
- $contig = $slice;
- } else {
- #we just want plain old contig coords
- $exon_start = $contig_start;
- $exon_end = $contig_end;
- $exon_strand = $contig_strand;
}
- #create an exon and add it to the prediction transcript
- $exon = Bio::EnsEMBL::Exon->new_fast($contig,
- $exon_start,
- $exon_end,
- $exon_strand);
- $exon->phase( $start_phase );
- $exon->end_phase( ($exon_end - $exon_start + 1 + $start_phase) % 3 );
- $exon->score( $score );
- $exon->p_value( $p_value );
-
- $prev_exon = $exon;
- $pre_trans->add_Exon($exon, $exon_rank - $already_merged);
- }
-
- #throw away last pred_transcript if it had no exons overlapping the slice
- if(@out) {
- if($slice && ( $transcript_slice_end < 1 ||
- $transcript_slice_start > $slice->length() )) {
- pop @out;
- } else {
- #set the stable id of the last prediction transcript
- $out[$#out]->stable_id("$stable_ctg.$stable_start.$stable_end");
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+
+ $slice = $dest_slice;
}
+
+ #finally, create the new repeat feature
+ push @ptranscripts, Bio::EnsEMBL::PredictionTranscript->new
+ ( '-start' => $seq_region_start,
+ '-end' => $seq_region_end,
+ '-strand' => $seq_region_strand,
+ '-adaptor' => $self,
+ '-slice' => $slice,
+ '-analysis' => $analysis,
+ '-dbID' => $prediction_transcript_id );
}
- return \@out;
+ return \@ptranscripts;
}
@@ -367,77 +292,61 @@ sub _objs_from_sth {
sub store {
my ( $self, @pre_transcripts ) = @_;
- my $exon_sql = q{
- INSERT INTO prediction_transcript ( prediction_transcript_id, exon_rank,
- contig_id, contig_start, contig_end,
- contig_strand, start_phase, score,
- p_value, analysis_id, exon_count )
- VALUES ( ?, ?, ?, ?, ?, ?, ?, ?, ?, ?, ? )
- };
+ my $ptstore_sth = $self->prepare
+ ("INSERT INTO prediction_transcript (seq_region_id, seq_region_start, " .
+ " seq_region_end, seq_region_strand, analysis_id) " .
+ "VALUES( ?, ?, ?, ?, ?)");
- my $exonst = $self->prepare($exon_sql);
+ my $db = $self->db();
+ my $analysis_adaptor = $db->get_AnalysisAdaptor();
+ my $pexon_adaptor = $db->get_PredictionExonAdaptor();
- foreach my $pre_trans (@pre_transcripts) {
- if( ! $pre_trans->isa('Bio::EnsEMBL::PredictionTranscript') ) {
- $self->throw("$pre_trans is not a EnsEMBL PredictionTranscript "
- . "- not dumping!");
+ FEATURE: foreach my $pt (@pre_transcripts) {
+ if(!ref($pt) || !$pt->isa('Bio::EnsEMBL::PredictionTranscript')) {
+ throw('Expected PredictionTranscript argument not [' . ref($pt).']');
}
-
- if( $pre_trans->dbID && $pre_trans->adaptor == $self ) {
- $self->warn("Already stored");
+
+ #skip prediction transcripts that have already been stored
+ if($pt->is_stored($db)) {
+ warning('Not storing already stored prediction transcript '. $pt->dbID);
+ next FEATURE;
}
-
- my $exonId = undef;
- my @pt_exons = @{$pre_trans->get_all_Exons()};
- my $dbID = undef;
- my $rank = 1;
-
- my @exons;
- foreach my $e (@pt_exons) {
- if($e && (!$e->contig || $e->contig->isa('Bio::EnsEMBL::Slice'))) {
- $self->throw('PredictionTranscript must be in contig coords to store');
- }
-
- if($e && $e->isa('Bio::EnsEMBL::StickyExon')) {
- push @exons, @{$e->get_all_component_Exons};
- } else {
- push @exons, $e;
- }
+
+ #get analysis and store it if it is not in the db
+ my $analysis = $pt->analysis();
+ if(!$analysis) {
+ throw('Prediction transcript must have analysis to be stored.');
+ }
+ if(!$analysis->is_stored($db)) {
+ $analysis_adaptor->store($analysis);
}
- for my $exon ( @exons ) {
- if( ! defined $exon ) { $rank++; next; }
-
- my $contig_id = $exon->contig->dbID();
- my $contig_start = $exon->start();
- my $contig_end = $exon->end();
- my $contig_strand = $exon->strand();
- my $start_phase = $exon->phase();
- my $end_phase = $exon->end_phase();
- my $score = $exon->score();
- my $p_value = $exon->p_value();
- my $analysis = $pre_trans->analysis->dbID;
-
- if( $rank == 1 ) {
- $exonst->execute( undef, 1, $contig_id, $contig_start,
- $contig_end, $contig_strand,
- $start_phase, $score, $p_value, $analysis,
- scalar( @exons ));
- $dbID = $exonst->{'mysql_insertid'};
- } else {
- $exonst->execute( $dbID, $rank, $contig_id, $contig_start,
- $contig_end, $contig_strand,
- $start_phase, $score, $p_value, $analysis,
- scalar( @exons ) );
- }
- $rank++;
+ #ensure that the transcript coordinates are correct, they may not be,
+ #if somebody has done some exon coordinate juggling and not recalculated
+ #the transcript coords.
+ $pt->recalculate_coordinates();
+
+ my $original = $pt;
+ my $seq_region_id;
+ ($pt, $seq_region_id) = $self->_pre_store($pt);
+
+ #store the prediction transcript
+ $ptstore_sth->execute($seq_region_id,
+ $pt->start(),
+ $pt->end(),
+ $pt->strand(),
+ $analysis->dbID());
+
+ my $pt_id = $ptstore_sth->{'mysql_insertid'};
+ $original->dbID($pt_id);
+ $original->adaptor($self);
+
+ #store the exons
+ my $rank = 1;
+ foreach my $pexon (@{$original->get_all_Exons}) {
+ $pexon_adaptor->store($pexon, $pt_id, $rank++);
}
-
- $pre_trans->dbID( $dbID );
- $pre_trans->adaptor( $self );
}
-
- $exonst->finish;
}
@@ -456,18 +365,30 @@ sub store {
sub remove {
my $self = shift;
my $pre_trans = shift;
-
- if ( ! defined $pre_trans->dbID() ) {
+
+ if(!ref($pre_trans)||!$pre_trans->isa('Bio::EnsEMBL::PredictionTranscript')){
+ throw('Expected PredictionTranscript argument.');
+ }
+
+ if(!$pre_trans->is_stored($self->db())) {
+ warning('PredictionTranscript is not stored in this DB - not removing.');
return;
}
- my $sth = $self->prepare( "DELETE FROM prediction_transcript
+ #remove all associated prediction exons
+ my $pexon_adaptor = $self->get_PredictionExonAdaptor();
+ foreach my $pexon (@{$pre_trans->get_all_Exons}) {
+ $pexon_adaptor->remove($pexon);
+ }
+
+ #remove the prediction transcript
+ my $sth = $self->prepare( "DELETE FROM prediction_transcript
WHERE prediction_transcript_id = ?" );
$sth->execute( $pre_trans->dbID );
- # uhh, didnt know another way of resetting to undef ...
- $pre_trans->{dbID} = undef;
- $pre_trans->{adaptor} = undef;
+ #unset the adaptor and internal id
+ $pre_trans->dbID(undef);
+ $pre_trans->adaptor(undef);
}
@@ -475,7 +396,8 @@ sub remove {
Arg [1] : none
Example : @feature_ids = @{$prediction_transcript_adaptor->list_dbIDs()};
- Description: Gets an array of internal ids for all prediction transcript features in the current db
+ Description: Gets an array of internal ids for all prediction transcript
+ features in the current db
Returntype : list of ints
Exceptions : none
Caller : ?
diff --git a/modules/Bio/EnsEMBL/DBSQL/ProteinAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/ProteinAdaptor.pm
index 567baa6850..c46b263b5a 100644
--- a/modules/Bio/EnsEMBL/DBSQL/ProteinAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/ProteinAdaptor.pm
@@ -12,193 +12,89 @@
=head1 NAME
-ProteinAdaptor - DESCRIPTION of Object
-
-=head1 SYNOPSIS
-
-use Bio::EnsEMBL::DBSQL::DBAdaptor;
-
-
-my $db = new Bio::EnsEMBL::DBSQL::DBAdaptor( -user => 'root',
- -db => 'pog' ,
- -host => 'caldy' ,
- -driver => 'mysql' );
-
-my $protein_adaptor = $db->get_ProteinAdaptor();
-
-my $protein = $protein_adaptor->fetch_by_transcript_id(1234);
-
-
+ProteinAdaptor - DEPRECATED Use TranslationAdaptor or TranscriptAdaptor instead
=head1 DESCRIPTION
-An adaptor which allows for the creation of Protein Objects from transcript
-or translation ids. There is no Protein table in the EnsEMBL core database
-and possibly this functionality should be moved into the translation object.
+DEPRECATED - Do not use this module. Use the TranslationAdaptor or
+TranscriptAdaptor instead. All Protein object functionality has been replaced
+on the Translation object so there is no reason to create Protein objects
+anymore.
=head1 CONTACT
-mongin@ebi.ac.uk
-
-=head1 APPENDIX
+ Post questions to the EnsEMBL development list
-The rest of the documentation details each of the object methods. Internal
-methods are usually preceded with a _
+=head1 METHODS
=cut
-
-# Let the code begin...
package Bio::EnsEMBL::DBSQL::ProteinAdaptor;
use vars qw(@ISA);
use strict;
-
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::DBSQL::TranslationAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
use Bio::EnsEMBL::Protein;
-@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+@ISA = qw(Bio::EnsEMBL::DBSQL::TranslationAdaptor);
=head2 fetch_by_transcript_id
- Arg [1] : int $transid
- The unique internal identifier of this proteins transcript
- Example : $protein = $protein_adaptor->fetch_by_transcript_id(1234);
- Description: (formerly fetch_Protein_by_transcriptId) Retrieves a protein
- object via the internal database identifier of its transcript
- Returntype : Bio::EnsEMBL::Protein
- Exceptions : none
- Caller : protview?
+ Description: DEPRECATED - use TranslationAdaptor::fetch_by_Transcript or
+ Transcript::translation instead
=cut
sub fetch_by_transcript_id{
- my ($self,$transid) = @_;
- my $query = "SELECT t.translation_id
- FROM transcript as t,
- transcript_stable_id as s
- WHERE s.stable_id = '$transid'
- AND t.transcript_id = s.transcript_id";
- my $sth = $self->prepare($query);
- $sth->execute();
- my @row = $sth->fetchrow;
-
- return $self->fetch_by_translation_id($row[0]);
- }
+ my ($self,$transid) = @_;
+ deprecate("Use Transcript::translation instead\n");
+ my $tr_adaptor = $self->db->get_TranscriptAdaptor();
+ my $tr = $tr_adaptor->fetch_by_dbID($transid);
+ my $tl = $tr->translation();
+ $tl->adaptor($self);
+ return bless $tl, 'Bio::EnsEMBL::Protein';
+}
=head2 fetch_by_translation_stable_id
- Arg [1] : int $transid
- the stable identifier of the translation of the desired protein
- Example : $prot = $pa->fetch_by_translation_stable_id('ENSP00000278194');
- Description: Creates a protein object using the translation table of the DB
- Returntype : Bio::EnsEMBL::Protein
- Exceptions : none
- Caller : protview?
+ Description: DEPRECATED - use TranslationAdaptor::fetch_by_stable_id
+ or Transcript::translation instead
=cut
sub fetch_by_translation_stable_id {
- my ($self,$transid) = @_;
- my $query = "SELECT translation_id
- FROM translation_stable_id
- WHERE stable_id = '$transid'";
- my $sth = $self->prepare($query);
- $sth->execute();
- my @row = $sth->fetchrow;
- return $self->fetch_by_translation_id($row[0]);
- }
-
+ my ($self,$transid) = @_;
+ deprecate("Use TranslationAdaptor::fetch_by_stable_id or " .
+ "Transcript::translation instead.");
+ my $tl_adaptor = $self->db->get_TranslationAdaptor();
+ my $tl = $tl_adaptor->fetch_by_stable_id($transid);
+ $tl->adaptor($self);
+ return bless $tl, 'Bio::EnsEMBL::Protein';
+}
=head2 fetch_by_translation_id
- Arg [1] : int $translation_id
- the unique DB identifier for the translation corresponding to
- the desired protein
- Example : $prot = $prot_adaptor->fetch_by_translation_id($id);
- Description: Retrieves a protein object from a database
- (formerly fetch_Protein_by_dbid)
- Returntype : Bio::EnsEMBL::Protein
- Exceptions : thrown if transcript or gene cannot be retrieved from database,
- or if the amino acid sequence cannot be obtained
- Caller : protview?
+ Description: DEPRECATED - use TranslationAdaptor::fetch_by_dbID or
=cut
sub fetch_by_translation_id {
my ($self, $translation_id) = @_;
-
- #Get the transcript id from the translation id
- my $query = "SELECT transcript_id,
- gene_id
- FROM transcript t
- WHERE translation_id = '$translation_id'";
-
- my $sth = $self->prepare($query);
- $sth ->execute();
-
- my ($transcript_id, $gene_id) = $sth->fetchrow();
-
- if (!defined $transcript_id) {
- $self->throw("$translation_id does not have a transcript_id");
- }
-
- if (!defined $gene_id) {
- $self->throw("$translation_id does not have a gene_id");
- }
-
- #Get Transcript object ( will allow us to get the aa sequence of the protein
- my $ta = $self->db()->get_TranscriptAdaptor();
- my $transcript = $ta->fetch_by_dbID($transcript_id);
-
- my $ga = $self->db()->get_GeneAdaptor();
- my $gene = $ga->fetch_by_dbID($gene_id);
+ deprecate("Use TranslationAdaptor::fetch_by_dbID or " .
+ "Transcript::translation instead.");
- #Get all of the family (at the Transcript level), not implemented yet
- #my $family = $self->fetch_Family_by_dbid($id);
-
- #Get the polypeptide sequence out of the transcript object
- my $sequence = $transcript->translate->seq;
-
- #Calculate the length of the Peptide
- my $length = length($sequence);
- if ($length == 0) {
- $self->throw("Transcript " . $transcript->stable_id() .
- " does not have an amino acid sequence");
- }
-
- #Define the moltype
- my $moltype = "protein";
- my $meta_obj = $self->db->get_MetaContainer();
- my $species = $meta_obj->get_Species();
- my $desc = "Protein predicted by Ensembl";
-
- #Create the Protein object
- my $protein = Bio::EnsEMBL::Protein->new ( -seq =>$sequence,
- -accession_number => $translation_id,
- -display_id => $translation_id,
- -primary_id => $translation_id,
- -id => $translation_id,
- -desc => $desc,
- -moltype => $moltype
- );
-
- #Set up the adaptor handler for the protein object
- $protein->adaptor($self);
-
- #Add the species object to protein object
- $protein->species($species);
-
- $protein->transcript($transcript);
- $protein->gene($gene);
-
- return $protein;
+ my $tl_adaptor = $self->db->get_TranslationAdaptor();
+ my $tl = $tl_adaptor->fetch_by_dbID($translation_id);
+ $tl->adaptor($self);
+ return bless $tl, 'Bio::EnsEMBL::Protein';
}
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/ProteinAlignFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/ProteinAlignFeatureAdaptor.pm
index a728e8ccca..d00ff2dac3 100644
--- a/modules/Bio/EnsEMBL/DBSQL/ProteinAlignFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/ProteinAlignFeatureAdaptor.pm
@@ -1,7 +1,5 @@
#
-# BioPerl module for Bio::EnsEMBL::DBSQL::ProteinAlignFeatureAdaptor
-#
-# Cared for by Ewan Birney <birney@ebi.ac.uk>
+# Ensembl module for Bio::EnsEMBL::DBSQL::ProteinAlignFeatureAdaptor
#
# Copyright Ewan Birney
#
@@ -16,108 +14,131 @@ Adaptor for ProteinAlignFeatures
=head1 SYNOPSIS
- $pfadp = $dbadaptor->get_ProteinAlignFeatureAdaptor();
+ $pafa = $dbadaptor->get_ProteinAlignFeatureAdaptor();
- my @features = $pfadp->fetch_by_Contig($contig);
+ my @features = @{$pafa->fetch_all_by_Slice($slice)};
- my @features = $pfadp->fetch_by_assembly_location($start,$end,$chr,'UCSC');
-
- $pfadp->store(@features);
+ $pafa->store(@features);
=head1 DESCRIPTION
-This is an adaptor for protein features on DNA sequence. Like other
-feature getting adaptors it has a number of fetch_ functions and a
-store function.
-
-
-=head1 AUTHOR - Ewan Birney
-
-Email birney@ebi.ac.uk
-
-Describe contact details here
+=head1 CONTACT
-=head1 APPENDIX
+Post questions to the Ensembl development list: ensembl-dev@ebia.ac.uk
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-# Let the code begin...
-
-
package Bio::EnsEMBL::DBSQL::ProteinAlignFeatureAdaptor;
use vars qw(@ISA);
use strict;
-# Object preamble - inherits from Bio::EnsEMBL::Root
-
use Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor;
use Bio::EnsEMBL::DnaPepAlignFeature;
-use Bio::EnsEMBL::SeqFeature;
+
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAlignFeatureAdaptor);
=head2 store
- Arg [1] : list of Bio::EnsEMBL::DnaPepAlignFeature @sf
- Example : $protein_align_feature_adaptor->store(@sf);
- Description: stores a list of features in the database
+ Arg [1] : list of Bio::EnsEMBL::DnaPepAlignFeature @feats
+ Example : $protein_align_feature_adaptor->store(@feats);
+ Description: stores a list of ProteinAlignFeatures in the database
Returntype : none
- Exceptions : thrown if there is no attached sequence on any of the features,
- if the features are not defined
- Caller :
+ Exceptions : throw if any of the provided features cannot be stored
+ which may occur if:
+ * The feature does not have an associated Slice
+ * The feature does not have an associated analysis
+ * The Slice the feature is associated with is on a seq_region
+ unknown to this database
+ A warning is given if:
+ * The feature has already been stored in this db
+ Caller : Pipeline
=cut
sub store{
- my ($self ,@sf) = @_;
+ my ($self, @feats) = @_;
+
+ throw("Must call store with features") if( scalar(@feats) == 0 );
+
+ my @tabs = $self->_tables;
+ my ($tablename) = @{$tabs[0]};
+
+ my $db = $self->db();
+ my $slice_adaptor = $db->get_SliceAdaptor();
+ my $analysis_adaptor = $db->get_AnalysisAdaptor();
+
+ my $sth = $self->prepare(
+ "INSERT INTO $tablename (seq_region_id, seq_region_start, seq_region_end,
+ seq_region_strand, hit_start, hit_end,
+ hit_name, cigar_line,
+ analysis_id, score, evalue, perc_ident)
+ VALUES (?,?,?,?,?,?,?,?,?,?, ?, ?)");
+
+ FEATURE: foreach my $feat ( @feats ) {
+ if( !ref $feat || !$feat->isa("Bio::EnsEMBL::DnaPepAlignFeature") ) {
+ throw("feature must be a Bio::EnsEMBL::DnaPepAlignFeature,"
+ . " not a [".ref($feat)."].");
+ }
+
+ if($feat->is_stored($db)) {
+ warning("PepDnaAlignFeature [".$feat->dbID."] is already stored" .
+ " in this database.");
+ next FEATURE;
+ }
+
+ #sanity check the hstart and hend
+ my $hstart = $feat->hstart();
+ my $hend = $feat->hend();
+ $self->_check_start_end_strand($hstart,$hend,1);
+
+ my $cigar_string = $feat->cigar_string();
+ if(!$cigar_string) {
+ $cigar_string = $feat->length() . 'M';
+ warning("DnaPepAlignFeature does not define a cigar_string.\n" .
+ "Assuming ungapped block with cigar_string=$cigar_string\n");
+ }
- if( scalar(@sf) == 0 ) {
- $self->throw("Must call store with list of sequence features");
+ my $hseqname = $feat->hseqname();
+ if(!$hseqname) {
+ throw("DnaPepAlignFeature must define an hseqname.");
}
- my $sth = $self->prepare(
- "INSERT INTO protein_align_feature(contig_id, contig_start, contig_end,
- contig_strand, hit_start, hit_end,
- hit_name, cigar_line, analysis_id,
- score, evalue, perc_ident)
- VALUES (?,?,?,?,?,?,?,?,?,?, ?, ?)");
-
- foreach my $sf ( @sf ) {
- if( !ref $sf || !$sf->isa("Bio::EnsEMBL::DnaPepAlignFeature") ) {
- $self->throw("Feature must be a Bio::EnsEMBL::DnaPepAlignFeature, " .
- "not a [$sf]");
- }
-
- if( !defined $sf->analysis ) {
- $self->throw("Cannot store sequence features without analysis");
- }
-
- # will only store if object is not already stored in this database
- $self->db()->get_AnalysisAdaptor()->store( $sf->analysis() );
-
- my $contig = $sf->entire_seq();
- #print STDERR $contig."\n";
- unless(defined $contig && $contig->isa("Bio::EnsEMBL::RawContig")) {
- $self->throw("Cannot store feature without Contig attached via " .
- "attach_seq\n");
- }
-
- $sth->execute($contig->dbID(), $sf->start, $sf->end,
- $sf->strand, $sf->hstart, $sf->hend,
- $sf->hseqname, $sf->cigar_string, $sf->analysis->dbID,
- $sf->score, $sf->p_value, $sf->percent_id);
-
- $sf->dbID($sth->{'mysql_insertid'});
+ if(!defined($feat->analysis)) {
+ throw("An analysis must be attached to the features to be stored.");
}
+
+ #store the analysis if it has not been stored yet
+ if(!$feat->analysis->is_stored($db)) {
+ $analysis_adaptor->store($feat->analysis());
+ }
+
+ my $slice = $feat->slice();
+ if(!defined($slice) || !$slice->isa("Bio::EnsEMBL::Slice")) {
+ throw("A slice must be attached to the features to be stored.");
+ }
+
+ my $original = $feat;
+ my $seq_region_id;
+ ($feat, $seq_region_id) = $self->_pre_store($feat);
+
+ $sth->execute( $seq_region_id, $feat->start, $feat->end, $feat->strand,
+ $feat->hstart, $feat->hend, $feat->hseqname,
+ $feat->cigar_string, $feat->analysis->dbID, $feat->score,
+ $feat->p_value, $feat->percent_id);
+ $original->dbID($sth->{'mysql_insertid'});
+ $original->adaptor($self);
}
+ $sth->finish();
+}
+
=head2 _objs_from_sth
@@ -135,124 +156,148 @@ sub store{
=cut
sub _objs_from_sth {
- my ($self, $sth, $mapper, $slice) = @_;
-
- my ($protein_align_feature_id, $contig_id, $contig_start, $contig_end,
- $analysis_id, $contig_strand, $hit_start, $hit_end, $hit_name,
- $cigar_line, $evalue, $perc_ident, $score);
-
- my $rca = $self->db()->get_RawContigAdaptor();
- my $aa = $self->db()->get_AnalysisAdaptor();
- my @features;
-
- my($analysis, $contig);
+ my ($self, $sth, $mapper, $dest_slice) = @_;
- my %a_hash;
- my %c_hash;
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
- my($row_cache, $row);
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $aa = $self->db->get_AnalysisAdaptor();
- $row_cache = $sth->fetchall_arrayref();
-
- if($slice) {
- my ($chr, $start, $end, $strand);
- my $slice_start = $slice->chr_start();
- my $slice_end = $slice->chr_end();
- my $slice_name = $slice->name();
- my $slice_strand = $slice->strand();
+ my @features;
+ my %analysis_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my ($protein_align_feature_id, $seq_region_id, $seq_region_start,
+ $seq_region_end, $analysis_id, $seq_region_strand, $hit_start,
+ $hit_end, $hit_name, $cigar_line, $evalue, $perc_ident, $score);
+
+ $sth->bind_columns(\$protein_align_feature_id, \$seq_region_id,
+ \$seq_region_start,\$seq_region_end, \$analysis_id,
+ \$seq_region_strand, \$hit_start,\$hit_end, \$hit_name,
+ \$cigar_line, \$evalue, \$perc_ident, \$score);
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
- my($feat_start, $feat_end, $feat_strand);
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
- while($row = shift @$row_cache) {
- ($protein_align_feature_id, $contig_id, $contig_start, $contig_end,
- $analysis_id, $contig_strand, $hit_start, $hit_end, $hit_name,
- $cigar_line, $evalue, $perc_ident, $score) = @$row;
+ FEATURE: while($sth->fetch()) {
+ #get the analysis object
+ my $analysis = $analysis_hash{$analysis_id} ||=
+ $aa->fetch_by_dbID($analysis_id);
- $analysis = $a_hash{$analysis_id} ||= $aa->fetch_by_dbID($analysis_id);
- ($chr, $start, $end, $strand) =
- $mapper->fast_to_assembly($contig_id, $contig_start,
- $contig_end, $contig_strand);
-
- #skip if feature maps to gap
- next unless(defined $start);
+ #get the slice object
+ my $slice = $slice_hash{"ID:".$seq_region_id};
- #skip if feature outside of slice area
- next if ($start > $slice_end) || ($end < $slice_start);
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
- #convert assembly coordinates to slice coordinates
- if($slice_strand == -1) {
- $feat_start = $slice_end - $end + 1;
- $feat_end = $slice_end - $start + 1;
- $feat_strand = $strand * -1;
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
} else {
- $feat_start = $start - $slice_start + 1;
- $feat_end = $end - $slice_start + 1;
- $feat_strand = $strand;
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
}
+ }
- #use a very fast (hack) constructor - normal object construction is too
- #slow for the number of features we are potentially dealing with
- push @features, Bio::EnsEMBL::DnaPepAlignFeature->new_fast(
- {'_gsf_tag_hash' => {},
- '_gsf_sub_array' => [],
- '_parse_h' => {},
- '_analysis' => $analysis,
- '_gsf_start' => $feat_start,
- '_gsf_end' => $feat_end,
- '_gsf_strand' => $feat_strand,
- '_gsf_score' => $score,
- '_seqname' => $slice_name,
- '_percent_id' => $perc_ident,
- '_p_value' => $evalue,
- '_hstart' => $hit_start,
- '_hend' => $hit_end,
- '_hstrand' => 1, #strand is always one for pep hits
- '_hseqname' => $hit_name,
- '_gsf_seq' => $slice,
- '_cigar_string' => $cigar_line,
- '_id' => $hit_name,
- '_database_id' => $protein_align_feature_id});
- }
-
- } else {
- while($row = shift @$row_cache) {
- ($protein_align_feature_id, $contig_id, $contig_start, $contig_end,
- $analysis_id, $contig_strand, $hit_start, $hit_end, $hit_name,
- $cigar_line, $evalue, $perc_ident, $score) = @$row;
-
- $analysis = $a_hash{$analysis_id} ||= $aa->fetch_by_dbID($analysis_id);
- $contig = $c_hash{$contig_id} ||= $rca->fetch_by_dbID($contig_id);
-
- #use a very fast (hack) constructor - normal object construction is too
- #slow for the number of features we are potentially dealing with
- push @features, Bio::EnsEMBL::DnaPepAlignFeature->new_fast(
- {'_gsf_tag_hash' => {},
- '_gsf_sub_array' => [],
- '_parse_h' => {},
- '_analysis' => $analysis,
- '_gsf_start' => $contig_start,
- '_gsf_end' => $contig_end,
- '_gsf_strand' => $contig_strand,
- '_gsf_score' => $score,
- '_seqname' => $contig->name,
- '_percent_id' => $perc_ident,
- '_p_value' => $evalue,
- '_hstart' => $hit_start,
- '_hend' => $hit_end,
- '_hstrand' => 1, #strand is always 1 for pep hits
- '_hseqname' => $hit_name,
- '_gsf_seq' => $contig,
- '_cigar_string' => $cigar_line,
- '_id' => $hit_name,
- '_database_id' => $protein_align_feature_id});
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+ $slice = $dest_slice;
}
- }
+ #finally, create the new dna align feature
+ push @features, Bio::EnsEMBL::DnaPepAlignFeature->new_fast
+ ( { 'slice' => $slice,
+ 'start' => $seq_region_start,
+ 'end' => $seq_region_end,
+ 'strand' => $seq_region_strand,
+ 'hseqname' => $hit_name,
+ 'hstart' => $hit_start,
+ 'hend' => $hit_end,
+ 'hstrand' => 1, #dna_pep_align features are always hstrand 1
+ 'score' => $score,
+ 'p_value' => $evalue,
+ 'percent_id' => $perc_ident,
+ 'cigar_string' => $cigar_line,
+ 'analysis' => $analysis,
+ 'adaptor' => $self,
+ 'dbID' => $protein_align_feature_id } );
+ }
return \@features;
}
+
sub _tables {
my $self = shift;
@@ -264,27 +309,28 @@ sub _columns {
my $self = shift;
#warning _objs_from_hashref method depends on ordering of this list
- return qw( paf.protein_align_feature_id
- paf.contig_id
- paf.contig_start
- paf.contig_end
- paf.analysis_id
- paf.contig_strand
- paf.hit_start
- paf.hit_end
- paf.hit_name
- paf.cigar_line
- paf.evalue
- paf.perc_ident
- paf.score );
+ return qw( paf.protein_align_feature_id
+ paf.seq_region_id
+ paf.seq_region_start
+ paf.seq_region_end
+ paf.analysis_id
+ paf.seq_region_strand
+ paf.hit_start
+ paf.hit_end
+ paf.hit_name
+ paf.cigar_line
+ paf.evalue
+ paf.perc_ident
+ paf.score );
}
=head2 list_dbIDs
Arg [1] : none
Example : @feature_ids = @{$protein_align_feature_adaptor->list_dbIDs()};
- Description: Gets an array of internal ids for all protein align features in the current db
- Returntype : list of ints
+ Description: Gets an array of internal ids for all protein align
+ features in the current db
+ Returntype : listref of ints
Exceptions : none
Caller : ?
diff --git a/modules/Bio/EnsEMBL/DBSQL/ProteinFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/ProteinFeatureAdaptor.pm
index 706a5ca7a6..b65ce4083e 100755
--- a/modules/Bio/EnsEMBL/DBSQL/ProteinFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/ProteinFeatureAdaptor.pm
@@ -1,5 +1,5 @@
#
-# EnsEMBL module for Bio::EnsEMBL::DBSQL::Feature_Obj
+# EnsEMBL module for Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor
#
# Cared for by Emmanuel Mongin <mongin@ebi.ac.uk>
#
@@ -11,58 +11,48 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::Feature_Obj - MySQL database adapter class for EnsEMBL
-Feature Objects
+Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor
=head1 SYNOPSIS
use Bio::EnsEMBL::DBSQL::DBAdaptor;
use Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor;
- $db = new Bio::EnsEMBL::DBSQL::DBAdaptor( -user => 'root', -dbname => 'pog' , -host => 'caldy' , -driver => 'mysql' );
- my $feature_obj=Bio::EnsEMBL::ProteinFeatureAdaptor->new($obj);
+ $db = new Bio::EnsEMBL::DBSQL::DBAdaptor( -user => 'root',
+ -db => 'pog' ,
+ -host => 'caldy' ,
+ -driver => 'mysql' );
+ my $pfa = $db->get_ProteinFeatureAdaptor();
-
-=head1 DESCRIPTION
+ my @prot_feats = $pfa->fetch_all_by_translation_id(1231);
-This object deals with protein feature objects. It contains methods to fetch
-prtein features from the database, write protein feature into the database,
-and delete these protein features from the databases.
+ my $prot_feat = $pfa->fetch_by_dbID(523);
-A protein feature is linked to a peptide. This linked is made through the
-translation id stored in the translation table. For example the method
-fetch_by_translationID will return all of the feature for this given peptide.
+
+=head1 DESCRIPTION
-The Obj object represents a database that is implemented somehow
-(you shouldn\'t care much as long as you can get the object).
=head1 CONTACT
-Emmanuel Mongin: mongin@ebi.ac.uk
-
-=head1 APPENDIX
+ Post questions to the EnsEMBl development mailing list :
+ ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods. Internal
-methods are usually preceded with a _
+=head1 METHODS
=cut
-# Let the code begin...
-
package Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor;
-use vars qw(@ISA);
use strict;
-# Object preamble - inheriets from Bio::EnsEMBL::DBSQL::BaseAdaptor
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
-
use Bio::EnsEMBL::ProteinFeature;
-use Bio::EnsEMBL::SeqFeature;
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate warning);
-@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
+use vars qw(@ISA);
+@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
=head2 fetch_all_by_translation_id
@@ -71,91 +61,69 @@ use Bio::EnsEMBL::SeqFeature;
the internal id of the translation corresponding to protein
whose features are desired
Example : @prot_feats = $prot_feat_adaptor->fetch_by_translation_id(1234);
- Description: Get all of the protein feature objects of a peptide
+ Description: Gets all protein features present on a peptide using the
+ translations internal identifier. This method will return
+ an unsorted list of all protein_feature types. The feature
+ types may be distinguished using the logic name attribute of
+ the attached analysis objects.
Returntype : listref of Bio::EnsEMBL::ProteinFeatures
Exceptions : none
Caller : ?
=cut
-sub fetch_by_translation_id {
- my($self,$transl) = @_;
+sub fetch_all_by_translation_id {
+ my($self,$translation_id) = @_;
+
+ if(!$translation_id) {
+ throw("translation_id argument is required\n");
+ }
my @features;
my $analysis_adaptor = $self->db()->get_AnalysisAdaptor();
-
-
- my $sth = $self->prepare(
- "SELECT p.seq_start, p.seq_end, p.analysis_id,
- p.score, p.perc_ident, p.evalue,
- p.hit_start, p.hit_end, p.hit_id,
- x.display_label, i.interpro_ac
- FROM protein_feature p,analysis a
- LEFT JOIN interpro AS i ON p.hit_id = i.id
- LEFT JOIN xref AS x ON x.dbprimary_acc = i.interpro_ac
- WHERE p.translation_id = ?
- AND p.analysis_id = a.analysis_id
- AND a.gff_feature = ?");
-
- $sth->execute("$transl", 'domain');
+
+ my $sth = $self->prepare
+ ("SELECT protein_feature_id, p.seq_start, p.seq_end, p.analysis_id, " .
+ " p.score, p.perc_ident, p.evalue, p.hit_start, p.hit_end, " .
+ " p.hit_id, x.display_label, i.interpro_ac " .
+ "FROM protein_feature p " .
+ "LEFT JOIN interpro AS i ON p.hit_id = i.id " .
+ "LEFT JOIN xref AS x ON x.dbprimary_acc = i.interpro_ac " .
+ "WHERE p.translation_id = ?");
+
+ $sth->execute($translation_id);
while( my $row = $sth->fetchrow_arrayref) {
- my ($start, $end, $analysisid, $score, $perc_id, $evalue, $hstart,
- $hend,$hid,$desc, $interpro_ac) = @$row;
-
+ my ($dbID, $start, $end, $analysisid, $score, $perc_id, $evalue, $hstart,
+ $hend,$hid,$desc, $interpro_ac) = @$row;
+
my $analysis = $analysis_adaptor->fetch_by_dbID($analysisid);
-
- my $feat = Bio::EnsEMBL::ProteinFeature->new();
- $feat->seqname($transl);
- $feat->start($start);
- $feat->end($end);
- $feat->analysis($analysis);
- $feat->percent_id($perc_id);
- $feat->p_value($evalue);
-
- $feat->hstart($hstart);
- $feat->hend($hend);
- $feat->hseqname($hid);
-
- $feat->idesc($desc);
- $feat->interpro_ac($interpro_ac);
+
+ if(!$analysis) {
+ warning("Analysis with dbID=$analysisid does not exist\n" .
+ "but is referenced by ProteinFeature $dbID");
+ }
+
+ my $feat = Bio::EnsEMBL::ProteinFeature->new
+ (-DBID => $dbID,
+ -ADADAPTOR => $self,
+ -SEQNAME => $translation_id,
+ -START => $start,
+ -END => $end,
+ -ANALYSIS => $analysis,
+ -PERCENT_ID => $perc_id,
+ -PVALUE => $evalue,
+ -HSTART => $hstart,
+ -HEND => $hend,
+ -HSEQNAME => $hid,
+ -IDESC => $desc,
+ -INTERPRO_AC => $interpro_ac);
push(@features,$feat);
}
-
- $sth = $self->prepare ("SELECT p.seq_start, p.seq_end, p.analysis_id,
- p.score, p.perc_ident, p.evalue,
- p.hit_start, p.hit_end, p.hit_id
- FROM protein_feature p, analysis a
- WHERE a.analysis_id = p.analysis_id
- AND p.translation_id = ?
- AND a.gff_feature != ?");
- $sth->execute("$transl", 'domain');
+ $sth->finish();
- while( my $row = $sth->fetchrow_arrayref) {
- my ($start,$end,$analysisid,$score,$perc_id,
- $evalue,$hstart,$hend,$hid) = @$row;
-
- my $analysis = $analysis_adaptor->fetch_by_dbID($analysisid);
-
-
- my $feat = Bio::EnsEMBL::ProteinFeature->new();
- $feat->seqname($transl);
- $feat->start($start);
- $feat->end($end);
- $feat->analysis($analysis);
- $feat->percent_id($perc_id);
- $feat->p_value($evalue);
-
- $feat->hstart($hstart);
- $feat->hend($hend);
- $feat->hseqname($hid);
-
-
- push(@features,$feat);
- }
-
return \@features;
}
@@ -173,316 +141,156 @@ sub fetch_by_translation_id {
=cut
sub fetch_by_dbID{
- my ($self,$protfeat_id) = @_;
-
- my $features;
- my $sth =
- $self->prepare("select * from protein_feature where id = '$protfeat_id'");
- my $res = $sth->execute;
-
- my $rowhash = $sth->fetchrow_hashref;
+ my ($self,$protfeat_id) = @_;
- if (!defined $rowhash->{'id'}) {
- $self->throw("This dbID: $protfeat_id, does not exist in the database");
- }
-
- my $feature = $self->_set_protein_feature($rowhash);
+ my $sth = $self->prepare(
+ "SELECT p.seq_start, p.seq_end, p.analysis_id, " .
+ " p.score, p.perc_ident, p.evalue, " .
+ " p.hit_start, p.hit_end, p.hit_id, " .
+ " x.display_label, i.interpro_ac " .
+ "FROM protein_feature p " .
+ "LEFT JOIN interpro AS i ON p.hit_id = i.id " .
+ "LEFT JOIN xref AS x ON x.dbprimary_acc = i.interpro_ac " .
+ "WHERE p.protein_feature_id = ?");
+
+ my $res = $sth->execute($protfeat_id);
- return $feature;
-}
-
-
-
-=head2 fetch_by_feature_and_dbID
-
- Arg [1] : string $feature
- analysis gff_source
- Arg [2] : int $transl
- unique translation identifier
- Example : none
- Description: Retrieves protein features of a protein using the dbID of the
- corresponding translation, and the analysis gff_source for
- the feature
- Returntype : listref of Bio::EnsEMBL::ProteinFeatures
- Exceptions : none
- Caller : ?
-
-=cut
-
-sub fetch_all_by_feature_and_dbID{
- my ($self,$feature,$transl) = @_;
- my @features;
- my $analysis_adaptor = $self->db()->get_AnalysisAdaptor();
-
- #The call has to be specific for the Interpro components because there
- #is one join to make on the interpro table and then with the xref table
- if (($feature eq "PRINTS") || ($feature eq "Pfam") ||
- ($feature eq "PROSITE") || ($feature eq "PROFILE")) {
-
- my $sth = $self->prepare("SELECT p.seq_start, p.seq_end, p.analysis_id,
- p.score, p.perc_ident, p.evalue,
- p.hit_start, p.hit_end, p.hit_id,
- x.display_label
- FROM protein_feature p,analysis a
- left join interpro as i on p.hit_id = i.id
- left join xref as x on x.dbprimary_acc = i.interpro_ac
- WHERE p.translation_id = '$transl'
- AND p.analysis_id = a.analysis_id
- AND a.gff_source = '$feature'");
-
- $sth->execute();
-
- while( my $arrayref = $sth->fetchrow_arrayref) {
- my ($start,$end,$analysisid,$score,$perc_id,$evalue,
- $hstart,$hend,$hid,$desc) = @{$arrayref};
-
- my $analysis = $analysis_adaptor->fetch_by_dbID($analysisid);
-
- my $feat1 = new Bio::EnsEMBL::SeqFeature ( -seqname => $transl,
- -start => $start,
- -end => $end,
- -score => $score,
- -analysis => $analysis,
- -percent_id => $perc_id,
- -p_value => $evalue);
-
- my $feat2 = new Bio::EnsEMBL::SeqFeature (-start => $hstart,
- -end => $hend,
- -analysis => $analysis,
- -seqname => $hid);
-
- my $feature = new Bio::EnsEMBL::ProteinFeature(-feature1 => $feat1,
- -feature2 => $feat2,);
-
- $feature->idesc($desc);
-
- if ($feature) {
- push(@features,$feature);
- }
-
- }
- }
+ if($sth->rows == 0) {
+ $sth->finish();
+ return undef;
+ }
- #Superfamily has also a description attached to it but there is
- #no join needed with the interpro table. but its also considered
- #as a domain feature
- elsif ($feature eq "superfamily") {
- my $sth = $self->prepare ("SELECT p.seq_start, p.seq_end, p.analysis_id,
- p.score, p.perc_ident, p.evalue,
- p.hit_start, p.hit_end, p.hit_id,
- x.display_label, x.dbprimary_acc
- FROM protein_feature AS p, analysis AS a,
- xref AS x
- WHERE a.gff_source = '$feature' AND
- p.translation_id = '$transl' AND
- a.analysis_id = p.analysis_id AND
- x.dbprimary_acc = p.hit_id");
- $sth->execute();
-
-
- while( my $arrayref = $sth->fetchrow_arrayref) {
-
- my ($start,$end,$analysisid,$score,$perc_id,$evalue,
- $hstart,$hend,$hid,$desc,$interproac) = @{$arrayref};
-
- my $analysis = $analysis_adaptor->fetch_by_dbID($analysisid);
-
- my $feat1 = new Bio::EnsEMBL::SeqFeature ( -seqname => $transl,
- -start => $start,
- -end => $end,
- -score => $score,
- -analysis => $analysis,
- -percent_id => $perc_id,
- -p_value => $evalue);
-
- my $feat2 = new Bio::EnsEMBL::SeqFeature (-start => $hstart,
- -end => $hend,
- -analysis => $analysis,
- -seqname => $hid);
-
- my $feature = new Bio::EnsEMBL::ProteinFeature(-feature1 => $feat1,
- -feature2 => $feat2,);
-
- $feature->idesc($desc);
- $feature->interpro_ac($interproac);
-
- if ($feature) {
- push(@features,$feature);
- }
-
- }
- }
-
- #Get all of the other features...Coils, TMHMM, ...
- else {
- my $sth = $self->prepare ("SELECT p.seq_start, p.seq_end, p.analysis_id,
- p.score, p.perc_ident, p.evalue,
- p.hit_start, p.hit_end, p.hit_id
- FROM protein_feature p,analysis a
- WHERE a.analysis_id = p.analysis_id
- AND p.translation_id = '$transl'
- AND a.gff_feature != 'domain'
- AND a.gff_source = '$feature'");
-
- $sth->execute();
- my @a = $sth->fetchrow();
-
- $sth->execute();
- while( my $arrayref = $sth->fetchrow_arrayref) {
- my ($start, $end, $analysisid, $score, $perc_id,
- $evalue, $hstart,$hend,$hid) = @{$arrayref};
-
- my $analysis = $analysis_adaptor->fetch_by_dbID($analysisid);
-
- my $feat1 = new Bio::EnsEMBL::SeqFeature ( -seqname => $transl,
- -start => $start,
- -end => $end,
- -score => $score,
- -analysis => $analysis,
- -percent_id => $perc_id,
- -p_value => $evalue);
-
- my $feat2 = new Bio::EnsEMBL::SeqFeature (-start => $hstart,
- -end => $hend,
- -analysis => $analysis,
- -seqname => $hid);
-
- my $feature = new Bio::EnsEMBL::ProteinFeature(-feature1 => $feat1,
- -feature2 => $feat2);
-
- if ($feature) {
- push(@features,$feature);
- }
- }
- }
-
- return \@features;
+ my ($start, $end, $analysis_id, $score, $perc_ident, $pvalue, $hstart,
+ $hend, $hseqname, $idesc, $interpro_ac) = $sth->fetchrow_array();
+
+ $sth->finish();
+
+ my $analysis = $self->db->get_AnalysisAdaptor->fetch_by_dbID($analysis_id);
+
+ return Bio::EnsEMBL::ProteinFeature->new
+ (-ADAPTOR => $self,
+ -DBID => $protfeat_id,
+ -START => $start,
+ -END => $end,
+ -HSTART => $hstart,
+ -HEND => $hend,
+ -HSEQNAME => $hseqname,
+ -ANALYSIS => $analysis,
+ -SCORE => $score,
+ -P_VALUE => $pvalue,
+ -PERCENT_ID => $perc_ident,
+ -IDESC => $idesc,
+ -INTERPRO_AC => $interpro_ac);
}
+
=head2 store
Arg [1] : Bio::EnsEMBL::ProteinFeature $feature
+ The feature to be stored
+ Arg [2] : int $translation_id
+
Example : $protein_feature_adaptor->store($protein_feature);
Description: Stores a protein feature in the database
- Returntype : none
- Exceptions : thrown if arg is not a Bio::EnsEMBL::SeqFeatureI
+ Returntype : int - the new internal identifier of the stored protein feature
+ Exceptions : thrown if arg is not a Bio::EnsEMBL:
Caller : none
=cut
sub store {
- my ($self,$feature) = @_;
- my $analysis;
-
-
- if( ! $feature->isa('Bio::EnsEMBL::FeaturePair') ) {
- $self->throw("A FeaturePair object required!");
- }
-
-
- eval {
- $feature->validate_prot_feature();
- };
-
- if (!defined($feature->analysis)) {
- $self->throw("Feature " . $feature->seqname .
- "doesn't have analysis. Can't write to database");
- } else {
- $analysis = $feature->analysis;
- }
-
-
- my $analysisid = $self->db->get_AnalysisAdaptor->store($analysis);
-
+ my ($self,$feature, $translation_id) = @_;
- my $homol = $feature->feature2;
-
- my $sth =
- $self->prepare("INSERT INTO protein_feature(protein_feature_id,
- translation_id, seq_start,
- seq_end, analysis_id,
- hit_start, hit_end,
- hit_id, score,
- perc_ident, evalue) ".
- "VALUES ('NULL',"
- ."'".$feature->seqname ."',"
- .$feature->start .","
- .$feature->end .","
- .$analysisid .","
- .$homol->start .","
- .$homol->end .","
- ."'".$homol->seqname ."',"
- .$feature->score .","
- .$feature->percent_id .","
- .$feature->p_value .")");
-
- $sth->execute();
+ if(!ref($feature) || !$feature->isa('Bio::EnsEMBL::ProteinFeature')) {
+ throw("ProteinFeature argument is required");
+ }
-}
+ if(!$translation_id) {
+ deprecate("Calling ProteinFeatureAdaptor without a translation_id is " .
+ "deprecated. Pass a translation_id argument rather than " .
+ "setting the ProteinFeature seqname to be the translation " .
+ "id");
+ $translation_id = $feature->seqname();
+ }
-=head2 remove
+ my $db = $self->db();
- Arg [1] : int $id
- the database id of the protein feature to remove
- Example : $protein_feature_adaptor->remove($prot_feat->dbID());
- Description: deletes a protein feature from the database
- Returntype : none
- Exceptions : none
- Caller : general
+ if($feature->is_stored($db)) {
+ warning("ProteinFeature " . $feature->dbID() . " is already stored in " .
+ "this database - not storing again");
+ }
-=cut
+ my $analysis = $feature->analysis();
+ if (!defined($analysis)) {
+ throw("Feature doesn't have analysis. Can't write to database");
+ }
-sub remove {
- my ($self,$id) = @_;
- my $sth = $self->prepare("delete from protein_feature where id = $id");
+ if(!$analysis->is_stored($db)) {
+ $db->get_AnalysisAdaptor->store($analysis);
+ }
- $sth->execute;
-}
+ my $sth =
+ $self->prepare("INSERT INTO protein_feature " .
+ " SET translation_id = ?, " .
+ " seq_start = ?, ".
+ " seq_end = ?, ".
+ " analysis_id = ?, ".
+ " hit_start = ?, ".
+ " hit_end = ?, ".
+ " hit_id = ?, ".
+ " score = ?, ".
+ " perc_ident = ?, ".
+ " evalue = ?");
+
+ $sth->execute($translation_id,
+ $feature->start(),
+ $feature->end(),
+ $analysis->dbID(),
+ $feature->hstart(),
+ $feature->hend(),
+ $feature->hseqname(),
+ $feature->score(),
+ $feature->percent_id(),
+ $feature->p_value());
+
+ my $dbID = $sth->{'mysql_insertid'};
-=head2 _set_protein_feature
+ $feature->adaptor($self);
+ $feature->dbID($dbID);
+
+ $sth->finish();
- Arg [1] : DBI row hash $rowhash
- Example : $prot_feat = $self->_set_protein_feature($row_hash);
- Description: PRIVATE creates a ProteinFeature object from a SQL hashref
- Returntype : Bio::EnsEMBL::ProteinFeature
- Exceptions : none
- Caller : internal
+ return $dbID;
+}
-=cut
-sub _set_protein_feature {
- my ($self,$rowhash) = @_;
- my $analysis =
- $self->db->get_AnalysisAdaptor->fetch_by_dbID($rowhash->{'analysis'});
-
- my $feat1 =
- new Bio::EnsEMBL::SeqFeature ( -seqname => $rowhash->{'translation'},
- -start => $rowhash->{'seq_start'},
- -end => $rowhash->{'seq_end'},
- -score => $rowhash->{'score'},
- -analysis => $analysis,
- -percent_id => $rowhash->{'perc_id'},
- -p_value => $rowhash->{'evalue'});
-
- my $feat2 = new Bio::EnsEMBL::SeqFeature (-start => $rowhash->{'hstart'},
- -end => $rowhash->{'hend'},
- -analysis => $analysis,
- -seqname => $rowhash->{'hid'});
-
- my $feature = new Bio::EnsEMBL::ProteinFeature(-feature1 => $feat1,
- -feature2 => $feat2,);
- return $feature;
+sub fetch_by_translation_id {
+ deprecate("Use fetch_all_by_translation_id instead.");
+ fetch_all_by_translation_id(@_);
}
+sub fetch_all_by_feature_and_dbID {
+ my $self = shift;
+ my $feature = shift;
+ my $translation_id = shift;
+ deprecate("Use fetch_all_by_translation_id instead.");
-1;
-
-
+ print STDERR "translation_id = $translation_id feature = $feature\n";
+ my $features = $self->fetch_all_by_translation_id($translation_id);
+ my @out;
+ foreach my $f (@$features) {
+ my $logic_name = lc($f->analysis->logic_name());
+ print STDERR "LOGIC_NAME = $logic_name | FEATURE = $feature\n";
+ push(@out, $f) if($logic_name eq lc($feature));
+ }
+ return \@out;
+}
+1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/ProxyAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/ProxyAdaptor.pm
index b1e81c0ae0..40b227736d 100644
--- a/modules/Bio/EnsEMBL/DBSQL/ProxyAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/ProxyAdaptor.pm
@@ -48,6 +48,7 @@ package Bio::EnsEMBL::DBSQL::ProxyAdaptor;
use strict;
use vars qw($AUTOLOAD @ISA);
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(throw);
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
@@ -74,7 +75,7 @@ sub new {
my $self = $class->SUPER::new($db);
unless($primary_adaptor) {
- $self->throw("The primary_adaptor argument is required\n");
+ throw("The primary_adaptor argument is required\n");
return undef;
}
@@ -148,8 +149,8 @@ sub AUTOLOAD {
}
#none of the attached adaptors could fulfill the request either
- $self->throw("The requested method $method could not be found in the "
- . $self->{'_proxy_type'} . " of the attached databases:" .
+ throw("The requested method $method could not be found in the "
+ . $self->{'_proxy_type'} . " of the attached databases:" .
@databases);
return undef;
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/RawContigAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/RawContigAdaptor.pm
index fd11a417b8..01a3ad939d 100644
--- a/modules/Bio/EnsEMBL/DBSQL/RawContigAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/RawContigAdaptor.pm
@@ -1,7 +1,6 @@
#
# EnsEMBL module for Bio::EnsEMBL::DBSQL::RawContigAdaptor
#
-# Cared for by Imre Vastrik <vastrik@ebi.ac.uk>
#
# Copyright Imre Vastrik
#
@@ -9,19 +8,8 @@
# POD documentation - main docs before the code
-=head1 NAME
-
-Bio::EnsEMBL::DBSQL::RawContigAdaptor - MySQL database adapter class
-for EnsEMBL RawContig Objects
-
-=head1 SYNOPSIS
-
-$contig_adaptor = $database_adaptor->get_RawContigAdaptor();
-$contig = $contig_adaptor->fetch_by_dbID(1234);
-
-=head1 DESCRIPTION
-
-Allows for the retrieval and storage of RawContig objects from the database.
+=head1 NAME Bio::EnsEMBL::DBSQL::RawContigAdaptor
+- DEPRECATED use Bio::EnsEMBL::DBSQL::SliceAdaptor instead
=head1 CONTACT
@@ -29,66 +17,37 @@ Post questions to the EnsEMBL developer list <ensembl-dev@ebi.ac.uk>
=cut
-package Bio::EnsEMBL::DBSQL::RawContigAdaptor;
-
-use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
-use DBI;
+package Bio::EnsEMBL::DBSQL::RawContigAdaptor;
+use vars qw(@ISA);
-use Bio::EnsEMBL::Clone;
+use Bio::EnsEMBL::DBSQL::SliceAdaptor;
use Bio::EnsEMBL::RawContig;
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate warning);
-use Bio::EnsEMBL::Utils::Cache; # CPAN LRU Cache module
-my $RAW_CONTIG_CACHE_SIZE = 20;
+@ISA = qw(Bio::EnsEMBL::DBSQL::SliceAdaptor);
-@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
-=head2 new
+=head2 fetch_by_dbID
- Arg [1] : list of arguments @args
- Example : $contig_adaptor = new Bio::EnsEMBL::RawContigAdaptor($db);
- Description: Creates a new RawContigAdaptor
- Returntype : Bio::EnsEMBL::RawContig
- Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::DBConnection
+ Description: DEPRECATED use SliceAdaptor::new instead
=cut
sub new {
- my($class, @args) = @_;
+ my $class = shift;
- #call super class constructor
- my $self = $class->SUPER::new(@args);
-
- #Initialize caching data structures
- tie(%{$self->{_raw_contig_cache}},
- 'Bio::EnsEMBL::Utils::Cache',
- $RAW_CONTIG_CACHE_SIZE);
-
- return $self;
+ deprecate("Bio::EnsEMBL::DBSQL::RawContigAdaptor is a deprecated class.\n" .
+ "Use the Bio::EnsEMBL::DBSQL::SliceAdaptor class instead");
+ return $class->SUPER::new(@_);
}
-
=head2 fetch_by_dbID
- Arg [1] : int $dbID
- the database identifier for the contig to retrieve
- Example : $contig = $raw_contig_adaptor->fetch_by_dbID(1234);
- Description: Retreives a RawContig object from the database. RawContigs
- retrieved by this method are in fact lazy loaded and not
- populated at all. Once data is actually requested from a
- RawContig object the attributes are pulled from the database.
- This is far faster for many requests which do not require
- populated Contigs. However, if a list of fully filled contigs
- is going to be required, then it may be much faster to use
- the fetch_filled_by_dbIDs method.
- Returntype : Bio::EnsEMBL::RawContig
- Exceptions : none
- Caller : general
+ Description: DEPRECATED use SliceAdaptor::fetch_by_seq_region_id instead
=cut
@@ -96,58 +55,39 @@ sub fetch_by_dbID {
my $self = shift;
my $dbID = shift;
- # retrieve the raw contig from the cache
- my $raw_contig = $self->{_raw_contig_cache}->{$dbID};
-
- unless($raw_contig) {
- # the cache did not contain a raw contig, create a new empty 'lazy loaded'
- # contig under the assumption that the contig data will not be
- # needed. If the data is needed then it can be loaded from the DB by
- # other db adaptor functions
+ deprecate('Use SliceAdaptor::fetch_by_seq_region_id instead');
- $raw_contig = new Bio::EnsEMBL::RawContig($dbID, $self);
+ my $contig = $self->fetch_by_seq_region_id($dbID);
- #store the new raw contig in the cache
- $self->{_raw_contig_cache}->{$dbID} = $raw_contig;
- }
-
- return $raw_contig;
+ #change blessing to contig so old contig functions still work
+ return bless($contig, 'Bio::EnsEMBL::RawContig');
}
=head2 fetch_all
- Arg [1] : none
- Example : @contigs = @{$raw_contig_adaptor->fetch_all()};
- Description: Retrieves all of the contig objects in the database. This will
- be a very slow request on a full-sized database
- Returntype : listref of Bio::EnsEMBL::RawContig
- Exceptions : none
- Caller : general
+ Description: DEPRECATED use SliceAdaptor::fetch_all instead
=cut
sub fetch_all {
my $self = shift;
- my @res;
- my $sth = $self->prepare( "SELECT contig_id, name, clone_id, length,
- embl_offset, dna_id
- FROM contig " );
- $sth->execute();
+ deprecate('Use SliceAdaptor::fetch_all instead');
+
+ my $result = $self->SUPER::fetch_all('seqlevel');
- return $self->_contig_from_sth( $sth );
+ #change blessings to RawContig so old contig functions still work
+ foreach my $contig (@$result) {
+ bless $contig, 'Bio::EnsEMBL::RawContig';
+ }
+
+ return $result;
}
=head2 fetch_by_name
- Arg [1] : string $name
- the name of the contig to retrieve
- Example : $contig = $rca->fetch_by_name('AC004501.1.1.39507');
- Description: Retrieves a contig from the database using the contig name
- Returntype : Bio::EnsEMBL::RawContig
- Exceptions : none
- Caller : general
+ Description: DEPRECATED use SliceAdaptor::fetch_by_region instead
=cut
@@ -155,76 +95,43 @@ sub fetch_by_name {
my $self = shift;
my $name = shift;
- my $sth = $self->prepare( "SELECT contig_id, name, clone_id, length,
- embl_offset, dna_id
- FROM contig
- WHERE name = '$name'" );
- $sth->execute();
-
- my ( $contig ) = @{$self->_contig_from_sth( $sth )};
+ deprecate('Use SliceAdaptor::fetch_by_region instead');
+
+ #assume that by 'contig' what is actually meant is seq level
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $cs = $csa->fetch_sequence_level();
- return $contig;
+ my $contig = $self->fetch_by_region($cs->name,
+ $name,
+ undef,
+ undef,
+ undef,
+ $cs->version);
+
+ #change blessing to RawContig so old contig functions still work
+ return bless $contig, 'Bio::EnsEMBL::RawContig';
}
=head2 fetch_filled_by_dbIDs
- Arg [1] : list of ints @contig_ids
- The list of contigs to retrieve
- Example : @contigs = $raw_contig_adaptor->fetch_filled_by_dbIDs(120, 121);
- Description: Returns a hashref of RawContigs retrieved from the database.
- The contigs are fully filled, and their attached clone is
- retrieved fully filled as well.
- Returntype : hasref of RawContigs, with dbIDs as keys
- Exceptions : none
- Caller : Bio::EnsEMBL::Slice::get_tiling_path
+ Description: DEPRECATED use SliceAdaptor::fetch_by_seq_region_id instead
=cut
sub fetch_filled_by_dbIDs {
my $self = shift;
my @contig_ids = @_;
- my %result = ();
- if(scalar(@contig_ids) == 0) {
- return ();
- }
+ deprecate('Use SliceAdaptor::fetch_by_seq_region_id instead');
+
+ my %result = ();
- my $sth = $self->prepare( "SELECT co.contig_id, co.name, co.clone_id,
- co.length, co.embl_offset, co.dna_id,
- cl.embl_acc, cl.embl_version,
- cl.name, cl.version, cl.htg_phase,
- UNIX_TIMESTAMP(cl.created), UNIX_TIMESTAMP(cl.modified)
- FROM contig co, clone cl
- WHERE co.contig_id IN ( " .
- join( ", ", @contig_ids ) . ")
- AND co.clone_id = cl.clone_id" );
-
-
-
- $sth->execute();
-
- while( my $aref = $sth->fetchrow_arrayref() ) {
- my($contig_id, $contig_name, $contig_clone_id, $contig_length,
- $contig_embl_offset, $contig_dna_id, $clone_embl_acc,
- $clone_embl_version, $clone_name, $clone_version, $clone_htg_phase,
- $clone_created, $clone_modified) = @$aref;
-
- my $contig = Bio::EnsEMBL::RawContig->new( $contig_id, $self );
- $self->_fill_contig_from_arrayref( $contig,$aref );
- my $clone = Bio::EnsEMBL::Clone->new
- (
- $self->db->get_CloneAdaptor(),
- $contig_clone_id, $clone_name,
- $clone_embl_acc, $clone_version, ,
- $clone_embl_version, $clone_htg_phase,
- $clone_created, $clone_modified
- );
- $contig->clone( $clone );
-
- $result{ $contig->dbID() } = $contig;
-
- $self->{_raw_contig_cache}->{$contig->dbID()} = $contig;
+ foreach my $contig_id (@contig_ids) {
+ my $contig = $self->fetch_by_seq_region_id($contig_id);
+ #change blessing to Bio::EnsEMBL::RawContig so old functions still work
+ bless $contig, 'Bio::EnsEMBL::RawContig';
+ $result{$contig_id} = $contig;
}
return \%result;
@@ -233,14 +140,7 @@ sub fetch_filled_by_dbIDs {
=head2 fetch_all_by_Clone
- Arg [1] : Bio::EnsEMBL::Clone $clone
- The clone object that the contigs are desired from
- Example : $contigs = raw_contig_adaptor->fetch_all_by_Clone($clone);
- Description: Returns a list reference of contig objects on a particular
- clone.
- Returntype : list reference of Bio::EnsEMBL::RawContig objects
- Exceptions : none
- Caller : general
+ Description: DEPRECATED use Slice::project instead
=cut
@@ -248,294 +148,64 @@ sub fetch_all_by_Clone {
my $self = shift;
my $clone = shift;
- my $clone_id = $clone->dbID;
-
- my $sth = $self->prepare( "SELECT contig_id, name, clone_id, length,
- embl_offset, dna_id
- FROM contig
- WHERE clone_id = $clone_id" );
+ deprecate('Use Slice::project instead');
- return $self->_contig_from_sth( $sth );
-}
+ #project this clone to contig coord system
+ #assume that by 'contig' what is actually meant is seq level
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $cs = $csa->fetch_sequence_level();
+ my $projection = $clone->project($cs->name, $cs->version);
+ my @out;
+ foreach my $seg (@$projection) {
+ my $contig = $seg->[2];
+ #rebless into contig so old method calls still work
+ bless $contig, 'Bio::EnsEMBL::RawContig';
+ push @out, $contig;
+ }
+ return \@out;
+}
=head2 fetch_attributes
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- Example : $raw_contig_adaptor->fetch_attributes($contig);
- Description: Fills in the attributes of a lazy-loaded RawContig object.
- RawContig.
- Returntype : none
- Exceptions : thrown if the attributes for a contig could not be filled
- Caller : Bio::EnsEMBL::RawContig
+ Description: DEPRECATED This method is no longer needed and does nothing
=cut
sub fetch_attributes {
- my $self = shift;
- my $contig = shift;
-
- my $dbID = $contig->dbID();
-
- unless($dbID && $contig->adaptor) {
- #do nothing, we do not know where to get the attributes from
- return;
- }
-
- my $sth = $self->prepare( "SELECT contig_id, name, clone_id, length,
- embl_offset, dna_id
- FROM contig
- WHERE contig_id = $dbID" );
- $sth->execute();
-
- my $aref = $sth->fetchrow_arrayref();
- if( defined $aref ) {
- $self->_fill_contig_from_arrayref( $contig, $aref );
- } else {
- $self->throw( "Couldnt fetch contig, unexpected .." );
- }
+ deprecate('This method is no longer needed and does nothing');
+ return;
}
=head2 store
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- The contig to store in the database.
- Arg [2] : Bio::EnsEMBL::Clone $clone
- The clone the contig is on
- Example : my $contig_id = $raw_contig_adaptor->store($contig, $clone);
- Description: Stores a contig and its associated DNA sequence in the database,
- returns the database id of the new database record. Attaches
- this adaptor to the the contig object and sets its dbID on
- success.
- Returntype : int
- Exceptions : thrown if $contig arg is not defined, $clone arg is not a clone
- or if the database insertion fails.
- Caller : Bio::EnsEMBL::Clone::store
+ Description: DEPRECATED XXX Replacement may need to be written
=cut
sub store {
- my ($self, $contig, $clone) = @_;
-
- unless($contig && $contig->isa('Bio::EnsEMBL::RawContig')) {
- $self->throw('contig arg is not a Bio::EnsEMBL::RawContig');
- }
- unless($clone && $clone->isa('Bio::EnsEMBL::Clone')) {
- $self->throw('clone arg is not a Bio::EnsEMBL::Clone');
- }
-
- my $date = $clone->created();
- my $dna = $contig->seq();
- my $dna_id = $self->db->get_SequenceAdaptor->store($dna, $date);
-
- my $sth = $self->prepare("INSERT INTO contig (name,
- clone_id,
- length,
- embl_offset,
- dna_id)
- VALUES ( ?, ?, ?, ?, ? )");
- my $rv = $sth->execute($contig->name(),
- $clone->dbID(),
- $contig->length(),
- $contig->embl_offset(),
- $dna_id);
-
- unless($rv) {
- $self->throw("Failed to insert contig " . $contig->name . "into database");
- }
-
- $sth->finish;
-
- $sth = $self->prepare("SELECT last_insert_id()");
- $sth->execute();
- my ($contig_id) = $sth->fetchrow_array();
-
- $sth->finish;
-
- $contig->dbID($contig_id);
- $contig->adaptor($self);
-
- return $contig_id;
-}
-
-
-=head2 _contig_from_sth
-
- Arg [1] : DBI Statementhandle $sth
- Example : @contigs = $self->_contig_from_sth($sth);
- Description: PRIVATE creates a contig from an executed DBI statement handle.
- Returntype : listref of Bio::EnsEMBL::RawContig
- Exceptions : thrown if the statement handle is not defined
- Caller : internal
-
-=cut
-
-sub _contig_from_sth {
- my $self = shift;
- my $sth = shift;
-
- if( !defined $sth ) {
- $self->throw("Bad internal error - no statement!");
- }
-
- my @res = ();
-
- $sth->execute();
- while( my $aref = $sth->fetchrow_arrayref() ) {
-
- my $contig = Bio::EnsEMBL::RawContig->new( $aref->[0], $self );
- $self->_fill_contig_from_arrayref( $contig, $aref );
-
- push( @res, $contig );
- }
-
- return \@res;
-}
-
-
-
-=head2 _fill_contig_from_arrayref
-
- Arg [1] : DBI array reference $aref
- Arg [2] : Bio::EnsEMBL::RawContig $contig
- Example : $contig = $self->_fill_contig_from_arrayref($arr_ref);
- Description: PRIVATE Populates an empty RawContig object from an
- array ref to an SQL query
- Returntype : Bio::EnsEMBL::RawContig
- Exceptions : thrown if
- Caller : internal
-
-=cut
-
-sub _fill_contig_from_arrayref {
- my $self = shift;
- my $contig = shift;
- my $aref = shift;
-
- if( !defined $aref ) {
- $self->throw("Bad internal error - no array ref");
- }
-
- my ( $contig_id, $name, $clone_id, $length, $offset, $dna_id) = @$aref;
-
-
- (defined $length) && $contig->length( $length );
- (defined $name) && $contig->name( $name );
- (defined $offset) && $contig->embl_offset( $offset );
-
- # maybe these should be lazy fetched...
- $contig->_clone_id($clone_id);
-
- #my $clone = $self->db->get_CloneAdaptor->fetch_by_dbID($clone_id);
- #$contig->clone( $clone );
-
-
- return $contig;
-}
-
-
-=head2 deleteObj
-
- Arg [1] : none
- Example : none
- Description: Cleans up object references contained within this object so
- that proper garbage collection can occur.
- Returntype : none
- Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::DBConnection::deleteObj
-
-=cut
-
-sub deleteObj {
- my $self = shift;
-
- #print STDERR "\t\tRawContigAdaptor::deleteObj\n";
-
- $self->SUPER::deleteObj();
-
- #flush internal cache
- %{$self->{'_raw_contig_cache'}} = ();
+ ### XXX Does a replacement need to be implemented?
+ deprecate('No replacement has been implemented');
+ return 0;
}
=head2 remove
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- Example : $rawcontig_adaptor->remove($contig)
- Description: This removes a contig (itself) plus any attached features and dna.
- The method loops over available 5 available feature adaptors and
- removes features on them individually.
- Returntype : none
- Exceptions : Throw if unable to get one of the feature adaptors.
- Throw if unable to identify the dna to remove.
- Throw if unable to remove record from dna table.
- Throw if unable to remove contig.
- Caller : Bio::EnsEMBL::DBSQL::CloneAdaptor
+ Description: DEPRECATED XXX Replacement may need to be written
=cut
sub remove {
- my ($self, $contig) = @_;
-
- # The list of feature adaptors to be looped over
- my @adaptor_list = ($self->db()->get_SimpleFeatureAdaptor(),
- $self->db()->get_RepeatFeatureAdaptor(),
- $self->db()->get_PredictionTranscriptAdaptor(),
- $self->db()->get_ProteinAlignFeatureAdaptor(),
- $self->db()->get_DnaAlignFeatureAdaptor());
-
- foreach my $adaptor ( @adaptor_list ) {
- $adaptor->remove_by_RawContig($contig);
- }
-
- # Delete DNA as long as we aren't using a remote DNA database.
- if ($self->db ne $self->db->dnadb) {
- $self->warn("Using a remote dna database - not deleting dna\n");
- } else {
-
- # Get the dna_id for the delete
- my $sth = $self->prepare("SELECT dna_id FROM contig where contig_id = ?");
- $sth->execute($contig->dbID);
- $self->throw("Failed to find any dna for dna_id '$contig->dbID'")
- unless $sth->rows;
-
- # Do the delete
- my $dna_id = $sth->fetchrow_array;
- $sth = $self->prepare("DELETE FROM dna WHERE dna_id = $dna_id");
- $sth->execute;
- my $flag_found=0;
- if($sth->rows){
- $flag_found=1;
- }
- # now try dnac - eval since table might not be present..
- $sth = $self->prepare("DELETE FROM dnac WHERE dna_id = $dna_id");
- eval{
- $sth->execute;
- };
- if($@){
- }else{
- if($sth->rows){
- $flag_found=1;
- }
- }
-
- if($flag_found==0){
- $self->throw("Failed to delete dna for dna_id '$dna_id'")
- }
- }
-
- # Remove the contig
- my $sth = $self->prepare("DELETE FROM contig WHERE contig_id = ?");
- $sth->execute($contig->dbID);
- $self->throw("Failed to delete contigs for contig_id '$contig->dbID'")
- unless $sth->rows;
-
+ ### XXX Does a replacement need to be implemented?
+ deprecate('No replacement has been implemened');
+ return 0;
}
@@ -543,14 +213,8 @@ sub remove {
sub fetch_all_names{
my ($self) = @_;
- my $sql = "select name from contig";
- my $sth = $self->prepare($sql);
- $sth->execute;
- my @names;
- while(my ($name) = $sth->fetchrow){
- push(@names, $name);
- }
-
+ deprecate('Use SliceAdaptor::fetch_all instead');
+ my @names = map {$_->seq_region_name} @{$self->fetch_all()};
return \@names;
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/RepeatConsensusAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/RepeatConsensusAdaptor.pm
index bba12c33b5..a3586b3463 100644
--- a/modules/Bio/EnsEMBL/DBSQL/RepeatConsensusAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/RepeatConsensusAdaptor.pm
@@ -11,9 +11,14 @@
Bio::EnsEMBL::DBSQL::RepeatConsensusAdaptor
-=head1 SYNOPSIS
+=head1
-$repeat_consensus_adaptor = $database_adaptor->get_RepeatConsensusAdaptor();
+$rca = $database_adaptor->get_RepeatConsensusAdaptor();
+
+$repeat_consensus = $rca->fetch_by_dbID(132);
+$repeat_consensus = $rca->fetch_by_name_class('AluSx', 'SINE/Alu');
+
+$rca->store($rc1, $rc2, $rc3);
=head1 DESCRIPTION
@@ -25,14 +30,9 @@ Email jgrg@ebi.ac.uk
=head1 CONTACT
-Arne Stabenau - stabenau@ebi.ac.uk
-Graham McVicker - mcvicker@ebi.ac.uk
-Ewan Birney - birney@ebi.ac.uk
-
-=head1 APPENDIX
+Post questions to the EnsEMBL developer list ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
@@ -41,8 +41,9 @@ package Bio::EnsEMBL::DBSQL::RepeatConsensusAdaptor;
use strict;
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
use Bio::EnsEMBL::RepeatConsensus;
-use vars qw(@ISA);
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate);
+use vars qw(@ISA);
@ISA = ('Bio::EnsEMBL::DBSQL::BaseAdaptor');
@@ -62,9 +63,9 @@ use vars qw(@ISA);
sub fetch_by_dbID {
my( $self, $db_id ) = @_;
- my ($rc) = @{$self->_generic_fetch("repeat_consensus_id = $db_id")};
+ my ($rc) = @{$self->_generic_fetch("repeat_consensus_id = $db_id")};
- return $rc;
+ return $rc;
}
@@ -84,7 +85,7 @@ sub fetch_by_dbID {
sub fetch_by_name {
my( $self, $name ) = @_;
- my ($rc) = @{$self->_generic_fetch("repeat_name = '$name'")};
+ my ($rc) = @{$self->_generic_fetch("repeat_name = '$name'")};
return $rc;
}
@@ -119,14 +120,14 @@ sub fetch_by_name_class {
}
-=head2 fetch_by_class_seq
+=head2 fetch_all_by_class_seq
Arg [1] : string $class
the class of the repeat consensus to obtain
Arg [2] : string $seq
the sequence of the repeat consensus to obtain
Example : $rc = $repeat_consensus_adaptor->
- fetch_by_name_class('trf', 'ATGGTGTCA');
+ fetch_all_by_class_seq('trf', 'ATGGTGTCA');
Description: Obtains a repeat consensus from the database
via its class and sequence
Returntype : listREF of Bio::EnsEMBL::RepeatConsensus
@@ -135,7 +136,7 @@ sub fetch_by_name_class {
=cut
-sub fetch_by_class_seq {
+sub fetch_all_by_class_seq {
my( $self, $class, $seq ) = @_;
return $self->_generic_fetch(qq{
@@ -145,6 +146,12 @@ sub fetch_by_class_seq {
}
+sub fetch_by_class_seq {
+ deprecate('Use fetch_all_by_class_seq instead');
+ fetch_all_by_class_seq(@_);
+}
+
+
=head2 _generic_fetch
Arg [1] : string $where_clause
@@ -159,37 +166,37 @@ sub fetch_by_class_seq {
sub _generic_fetch {
my( $self, $where_clause ) = @_;
-
- my( $repeat_consensus_id,
- $repeat_name,
- $repeat_class,
- $repeat_length,
- );
-
+
+ my( $repeat_consensus_id, $repeat_name, $repeat_class,$repeat_length,
+ $repeat_consensus );
+
my $sth = $self->prepare(qq{
SELECT repeat_consensus_id
, repeat_name
, repeat_class
, LENGTH(repeat_consensus)
+ , repeat_consensus
FROM repeat_consensus
WHERE }. $where_clause);
+
$sth->execute;
$sth->bind_columns(
\$repeat_consensus_id,
\$repeat_name,
\$repeat_class,
\$repeat_length,
+ \$repeat_consensus
);
-
- my( @consensi );
+
+ my @consensi;
while ($sth->fetch) {
- my $rc = Bio::EnsEMBL::RepeatConsensus->new;
- $rc->dbID($repeat_consensus_id);
- $rc->name($repeat_name);
- $rc->repeat_class($repeat_class);
- $rc->length($repeat_length);
- $rc->adaptor($self);
- push(@consensi, $rc);
+ push @consensi, Bio::EnsEMBL::RepeatConsensus->new
+ (-DBID => $repeat_consensus_id,
+ -NAME => $repeat_name,
+ -REPEAT_CLASS => $repeat_class,
+ -LENGTH => $repeat_length,
+ -ADAPTOR => $self,
+ -REPEAT_CONSENSUS => $repeat_consensus);
}
return \@consensi;
}
@@ -208,7 +215,7 @@ sub _generic_fetch {
sub store {
my( $self, @consensi ) = @_;
-
+
my $sth = $self->prepare(q{
INSERT into repeat_consensus( repeat_consensus_id
, repeat_name
@@ -216,20 +223,20 @@ sub store {
, repeat_consensus )
VALUES (NULL, ?,?,?)
});
-
+
foreach my $rc (@consensi) {
my $name = $rc->name
- or $self->throw("name not set");
+ or throw("name not set");
my $class = $rc->repeat_class
- or $self->throw("repeat_class not set");
+ or throw("repeat_class not set");
my $seq = $rc->repeat_consensus
- or $self->throw("repeat_consensus not set");
-
+ or throw("repeat_consensus not set");
+
$sth->execute($name, $class, $seq);
-
+
my $db_id = $sth->{'mysql_insertid'}
- or $self->throw("Didn't get an insertid from the INSERT statement");
-
+ or throw("Didn't get an insertid from the INSERT statement");
+
$rc->dbID($db_id);
$rc->adaptor($self);
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/RepeatFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/RepeatFeatureAdaptor.pm
index da83a31378..26db9489d3 100644
--- a/modules/Bio/EnsEMBL/DBSQL/RepeatFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/RepeatFeatureAdaptor.pm
@@ -13,56 +13,49 @@ Bio::EnsEMBL::DBSQL::RepeatFeatureAdaptor
=head1 SYNOPSIS
-$repeat_feature_adaptor = $database_adaptor->get_RepeatFeatureAdaptor();
+$rfa = $database_adaptor->get_RepeatFeatureAdaptor();
+
+my $repeat = $rfa->fetch_by_dbID(1234);
+my @repeats = @{$rfa->fetch_all_by_Slice($slice)};
=head1 DESCRIPTION
This is an adaptor for the retrieval and storage of RepeatFeature objects
from the RepeatFeature database. Most of the implementation is in the
-superclass BaseFeatureAdaptor.
+superclass BaseFeatureAdaptor.
=head1 AUTHOR - James Gilbert
-Email jgrg@ebi.ac.uk
-
=head1 CONTACT
-Arne Stabenau - stabenau@ebi.ac.uk
-Graham McVicker - mcvicker@ebi.ac.uk
-Ewan Birney - birney@ebi.ac.uk
-
-=head1 APPENDIX
+Post questions to the EnsEMBL developer list: ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-
-# Let the code begin...
-
package Bio::EnsEMBL::DBSQL::RepeatFeatureAdaptor;
use strict;
use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
use Bio::EnsEMBL::RepeatFeature;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
use vars qw(@ISA);
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
-=head2 _tablename
-
- Arg [1] : none
- Example : none
- Description: PROTECTED Implementation of abstract superclass method to
- provide the name of the tables to query
- Returntype : string
- Exceptions : none
- Caller : internal
+# _tablename
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED Implementation of abstract superclass method to
+# provide the name of the tables to query
+# Returntype : string
+# Exceptions : none
+# Caller : internal
-=cut
sub _tables {
my $self = shift;
@@ -71,26 +64,24 @@ sub _tables {
}
-=head2 _columns
-
- Arg [1] : none
- Example : none
- Description: PROTECTED Implementation of abstract superclass method to
- provide the name of the columns to query
- Returntype : list of strings
- Exceptions : none
- Caller : internal
-
-=cut
+# _columns
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED Implementation of abstract superclass method to
+# provide the name of the columns to query
+# Returntype : list of strings
+# Exceptions : none
+# Caller : internal
sub _columns {
my $self = shift;
-
+
return qw (r.repeat_feature_id
- r.contig_id
- r.contig_start
- r.contig_end
- r.contig_strand
+ r.seq_region_id
+ r.seq_region_start
+ r.seq_region_end
+ r.seq_region_strand
r.repeat_consensus_id
r.repeat_start
r.repeat_end
@@ -102,18 +93,15 @@ sub _columns {
}
-
-=head2 _default_where_clause
-
- Arg [1] : none
- Example : none
- Description: Overrides superclass method to provide an additional
- table joining constraint before the SQL query is performed.
- Returntype : string
- Exceptions : none
- Caller : generic_fetch
-
-=cut
+# _default_where_clause
+# Arg [1] : none
+# Example : none
+# Description: Overrides superclass method to provide an additional
+# table joining constraint before the SQL query is performed.
+# Returntype : string
+# Exceptions : none
+# Caller : generic_fetch
+#
sub _default_where_clause {
my $self = shift;
@@ -122,90 +110,161 @@ sub _default_where_clause {
}
-=head2 _obj_from_hashref
- Arg [1] : Hashreference $hashref
- Example : none
- Description: PROTECTED implementation of abstract superclass method.
- responsible for the creation of RepeatFeatures from a
- hashref generated from an SQL query
- Returntype : listref of Bio::EnsEMBL::RepeatFeatures in contig coordinates
- Exceptions : none
- Caller : internal
-
-=cut
+# Description: PROTECTED implementation of abstract superclass method.
+# responsible for the creation of RepeatFeatures from a
+# hashref generated from an SQL query
sub _objs_from_sth {
- my ($self, $sth) = @_;
+ my ($self, $sth, $mapper, $dest_slice) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
my $rca = $self->db()->get_RepeatConsensusAdaptor();
- my $ca = $self->db()->get_RawContigAdaptor();
+ my $sa = $self->db()->get_SliceAdaptor();
my $aa = $self->db->get_AnalysisAdaptor();
my @features;
my %rc_hash;
my %analysis_hash;
- my %contig_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
- my($repeat_feature_id, $contig_id, $contig_start, $contig_end,
- $contig_strand, $repeat_consensus_id, $repeat_start, $repeat_end,
+ my($repeat_feature_id, $seq_region_id, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $repeat_consensus_id, $repeat_start, $repeat_end,
$analysis_id, $score, $repeat_name, $repeat_class,
$repeat_consensus);
-
- $sth->bind_columns( \$repeat_feature_id, \$contig_id, \$contig_start,
- \$contig_end, \$contig_strand, \$repeat_consensus_id,
- \$repeat_start,\$repeat_end, \$analysis_id, \$score,
- \$repeat_name, \$repeat_class,
+
+ $sth->bind_columns( \$repeat_feature_id, \$seq_region_id, \$seq_region_start,
+ \$seq_region_end, \$seq_region_strand,
+ \$repeat_consensus_id, \$repeat_start,\$repeat_end,
+ \$analysis_id, \$score, \$repeat_name, \$repeat_class,
\$repeat_consensus );
- my $rc;
- my $contig;
- my $analysis;
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
- while($sth->fetch()) {
+ FEATURE: while($sth->fetch()) {
#create a repeat consensus object
- unless($rc = $rc_hash{$repeat_consensus_id}) {
- $rc = new Bio::EnsEMBL::RepeatConsensus;
- $rc->dbID($repeat_consensus_id);
- $rc->repeat_class($repeat_class);
- $rc->name($repeat_name);
- $rc->repeat_consensus($repeat_consensus);
- $rc->adaptor($rca);
-
- $rc_hash{$repeat_consensus_id} = $rc;
+ my $rc = $rc_hash{$repeat_consensus_id} ||=
+ Bio::EnsEMBL::RepeatConsensus->new
+ (-DBID => $repeat_consensus_id,
+ -ADAPTOR => $rca,
+ -NAME => $repeat_name,
+ -REPEAT_CLASS => $repeat_class,
+ -REPEAT_CONSENSUS => $repeat_consensus,
+ -LENGTH => length($repeat_consensus));
+
+ #get the analysis object
+ my $analysis = $analysis_hash{$analysis_id} ||=
+ $aa->fetch_by_dbID($analysis_id);
+
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
}
-
- unless($analysis = $analysis_hash{$analysis_id}) {
- $analysis = $aa->fetch_by_dbID($analysis_id);
- $analysis_hash{$analysis_id} = $analysis;
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
}
- unless($contig = $contig_hash{$contig_id}) {
- $contig = $ca->fetch_by_dbID($contig_id);
- $contig_hash{$contig_id} = $contig;
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+ $slice = $dest_slice;
}
- #create the new repeat feature
- push @features, Bio::EnsEMBL::RepeatFeature->new_fast(
- { '_gsf_tag_hash' => {},
- '_gsf_sub_array' => [],
- '_parse_h' => {},
- '_analysis' => $analysis,
- '_gsf_start' => $contig_start,
- '_gsf_end' => $contig_end,
- '_gsf_strand' => $contig_strand,
- '_gsf_score' => $score,
- '_hstart' => $repeat_start,
- '_hend' => $repeat_end,
- '_repeat_consensus' => $rc,
- '_adaptor' => $self,
- '_gsf_seq' => $contig,
- '_db_id' => $repeat_feature_id } );
+ #finally, create the new repeat feature
+ push @features, Bio::EnsEMBL::RepeatFeature->new_fast
+ ( { 'analysis' => $analysis,
+ 'start' => $seq_region_start,
+ 'end' => $seq_region_end,
+ 'strand' => $seq_region_strand,
+ 'score' => $score,
+ 'hstart' => $repeat_start,
+ 'hend' => $repeat_end,
+ 'repeat_consensus' => $rc,
+ 'adaptor' => $self,
+ 'slice' => $slice,
+ 'dbID' => $repeat_feature_id } );
}
return \@features;
}
-
+
=head2 store
@@ -222,16 +281,18 @@ sub _objs_from_sth {
sub store {
my( $self, @repeats ) = @_;
-
- my $rca = $self->db->get_RepeatConsensusAdaptor;
+
+ my $db = $self->db();
+ my $rca = $db->get_RepeatConsensusAdaptor();
+ my $sa = $db->get_SliceAdaptor();
my ($cons, $db_id);
my $sth = $self->prepare(qq{
INSERT into repeat_feature( repeat_feature_id
- , contig_id
- , contig_start
- , contig_end
- , contig_strand
+ , seq_region_id
+ , seq_region_start
+ , seq_region_end
+ , seq_region_strand
, repeat_consensus_id
, repeat_start
, repeat_end
@@ -240,10 +301,19 @@ sub store {
VALUES(NULL, ?,?,?,?,?,?,?,?,?)
});
- foreach my $rf (@repeats) {
- $self->throw("Must have a RepeatConsensus attached")
- unless defined ($cons = $rf->repeat_consensus);
-
+ FEATURE: foreach my $rf (@repeats) {
+ if(!ref($rf) || !$rf->isa('Bio::EnsEMBL::RepeatFeature')) {
+ throw('Expected RepeatFeature argument not [' . ref($rf) .'].');
+ }
+
+ if($rf->is_stored($db)) {
+ warning("RepeatFeature [".$rf->dbID."] is already stored in this DB.");
+ next FEATURE;
+ }
+
+ my $cons = $rf->repeat_consensus();
+ throw("Must have a RepeatConsensus attached") if(!defined($cons));
+
# for tandem repeats - simply store consensus and repeat
# one pair per hit. don't need to check consensi stored
# already. consensus has name and class set to 'trf'
@@ -251,12 +321,12 @@ sub store {
if ($cons->repeat_class eq 'trf') {
# Look for matches already stored
- my @match = @{$rca->fetch_by_class_seq('trf', $cons->repeat_consensus)};
+ my @match = @{$rca->fetch_by_class_seq('trf', $cons->repeat_consensus)};
if (@match) {
- $cons->dbID($match[0]->dbID());
+ $cons->dbID($match[0]->dbID());
}
else {
- $rca->store($cons);
+ $rca->store($cons);
}
} elsif ($cons->repeat_class eq 'Simple_repeat') {
@@ -266,67 +336,63 @@ sub store {
$cons->repeat_consensus($rcon);
# Look for matches already stored
- my $match = $rca->fetch_by_name_class($cons->name, 'Simple_repeat');
+ my $match = $rca->fetch_by_name_class($cons->name, 'Simple_repeat');
if ($match) {
- $cons->dbID($match->dbID());
+ $cons->dbID($match->dbID());
}
else {
- $rca->store($cons);
+ $rca->store($cons);
}
} else {
# for other repeats - need to see if a consensus is stored already
- unless ($cons->dbID) {
- my $match = ($rca->fetch_by_name($cons->name));
+ if(!$cons->dbID) {
+ my $match = ($rca->fetch_by_name($cons->name));
- if($match) {
- #set the consensus dbID to be the same as the database one
- $cons->dbID($match->dbID());
- } else {
- # if we don't match a consensus already stored create a fake one
- # and set consensus to 'N' as null seq not allowed
- # FIXME: not happy with this, but ho hum ...
- $self->warn("Can't find " . $cons->name . "\n");
- $cons->repeat_consensus("N");
- $rca->store($cons);
- }
-
- #if (@match > 1) {
- #multiple consensi were matched
- # $self->warn(@match . " consensi for " . $cons->name . "\n");
- #}
+ if($match) {
+ #set the consensus dbID to be the same as the database one
+ $cons->dbID($match->dbID());
+ } else {
+ # if we don't match a consensus already stored create a fake one
+ # and set consensus to 'N' as null seq not allowed
+ # FIXME: not happy with this, but ho hum ...
+ warning("Can't find " . $cons->name . "\n");
+ $cons->repeat_consensus("N");
+ $rca->store($cons);
+ }
}
+
+ #if (@match > 1) {
+ #multiple consensi were matched
+ # $self->warn(@match . " consensi for " . $cons->name . "\n");
+ #}
}
-
- my $contig = $rf->entire_seq();
-
- unless(defined $contig && $contig->isa("Bio::EnsEMBL::RawContig")) {
- $self->throw("RepeatFeature cannot be stored without a contig " .
- "attached via the attach_seq method");
- } unless($contig->dbID()) {
- $self->throw("RepeatFeature cannot be stored because attached contig " .
- "does not have a dbID");
+
+ my $slice = $rf->slice();
+ if(!ref($slice) || !$slice->isa("Bio::EnsEMBL::Slice")) {
+ throw("RepeatFeature cannot be stored without an associated slice.");
}
-
- # store analysis if not there already
- $self->db->get_AnalysisAdaptor->store($rf->analysis);
-
- $sth->execute(
- $contig->dbID(),
- $rf->start,
- $rf->end,
- $rf->strand,
- $rf->repeat_consensus->dbID(),
- $rf->hstart,
- $rf->hend,
- $rf->score,
- $rf->analysis->dbID,
- );
+
+ my $original = $rf;
+ my $seq_region_id;
+ ($rf, $seq_region_id) = $self->_pre_store($rf);
+
+ $sth->execute($seq_region_id,
+ $rf->start,
+ $rf->end,
+ $rf->strand,
+ $rf->repeat_consensus->dbID(),
+ $rf->hstart,
+ $rf->hend,
+ $rf->score,
+ $rf->analysis->dbID);
my $db_id = $sth->{'mysql_insertid'}
- or $self->throw("Didn't get an insertid from the INSERT statement");
- $rf->dbID($db_id);
+ or throw("Didn't get an insertid from the INSERT statement");
+
+ $original->dbID($db_id);
+ $original->adaptor($self);
}
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/SequenceAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/SequenceAdaptor.pm
index 1c9aabbb1c..6af66ae180 100644
--- a/modules/Bio/EnsEMBL/DBSQL/SequenceAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/SequenceAdaptor.pm
@@ -1,5 +1,5 @@
#
-# EnsEMBL module for Bio::EnsEMBL::DSQL::SequenceAdaptor
+# EnsEMBL module for Bio::EnsEMBL::DBSQL::SequenceAdaptor
#
# Cared for by Arne Stabenau <stabenau@ebi.ac.uk>
#
@@ -7,8 +7,6 @@
#
# You may distribute this module under the same terms as perl itself
-# POD documentation - main docs before the code
-
=head1 NAME
Bio::EnsEMBL::DBSQL::SequenceAdaptor - produce sequence strings from locations
@@ -16,206 +14,48 @@ Bio::EnsEMBL::DBSQL::SequenceAdaptor - produce sequence strings from locations
=head1 SYNOPSIS
$seq_adptr = $database_adaptor->get_SequenceAdaptor();
-$dna = $seq_adptr->fetch_by_RawContig_start_end_strand($contig, 1, 1000, -1);
+$dna = ${$seq_adptr->fetch_by_Slice_start_end_strand($slice, 1, 1000, -1);}
=head1 DESCRIPTION
-An adaptor for the retrieval of sequences of DNA from the database
+An adaptor for the retrieval of DNA sequence from the EnsEMBL database
=head1 CONTACT
-Arne Stabenau - stabenau@ebi.ac.uk
-Elia Stupka - elia@fugu-sg.org
-
-=head1 APPENDIX
+Post questions/comments to the EnsEMBL development list:
+ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-
-# Let the code begin...
package Bio::EnsEMBL::DBSQL::SequenceAdaptor;
+
use vars qw(@ISA);
use strict;
-# Object preamble - inheriets from Bio::Root::Object
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
-
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate);
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseAdaptor);
-
-=head2 fetch_by_RawContig_start_end_strand
-
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- Arg [2] : int $start
- Arg [3] : int $end
- a -1 means until the end
- Arg [4] : int $strand
- -1, 1 are possible values
- Arg [5] : (optional) force read from compressed DNA table
- Example : $dna = $seq_adp->fetch_by_RawContig_start_end_strand($contig, 1,
- 1000, -1);
- Description: retrieves the dna string from the database from the
- given RawContig.
- Returntype : string
- Exceptions : thrown if start < 1
- Caller : Bio::EnsEMBL::RawContig::seq(), RawContig::subseq()
-
-=cut
-
-sub fetch_by_RawContig_start_end_strand {
- my ( $self, $contig, $start, $end, $strand, $compressed ) = @_;
- my $sth;
-
- $compressed ||= 0;
-
- if( $start < 1 ) {
- $self->throw( "Wrong parameters" );
- }
-
- unless($contig && ref $contig && $contig->isa("Bio::EnsEMBL::RawContig")) {
- $self->throw("contig arg must be contig not a [$contig]");
- }
-
- {
-
- my $query;
-
- if($compressed==1 || $self->is_compressed){
-
- # query for compressed mode, uses hex and a different range
- # (compression implemented by th, 14/7/03)
- # needs to getback dna_id as will have to check for Ns in dnan table
- my $start4=int(($start-1)/4)+1;
-
- if( $end == -1 ) {
- $query = "SELECT c.length, HEX( SUBSTRING( d.sequence, $start4 ) ),
- d.n_line
- FROM dnac d, contig c
- WHERE d.dna_id = c.dna_id
- AND c.contig_id = ?";
-
- } else {
- my $end4=int(($end-1)/4)+1;
- my $length4 = $end4 - $start4 + 1;
- if( $length4 < 1 ) {
- $self->throw( "Wrong parameters" );
- }
-
- $query = "SELECT c.length, HEX( SUBSTRING( d.sequence, $start4, $length4 ) ),
- d.n_line
- FROM dnac d, contig c
- WHERE d.dna_id = c.dna_id
- AND c.contig_id = ?";
- }
-
- }else{
-
- if( $end == -1 ) {
-
- $query = "SELECT c.length, SUBSTRING( d.sequence, $start )
- FROM dna d, contig c
- WHERE d.dna_id = c.dna_id
- AND c.contig_id = ?";
-
- } else {
- my $length = $end - $start + 1;
- if( $length < 1 ) {
- $self->throw( "Wrong parameters" );
- }
-
- $query = "SELECT c.length, SUBSTRING( d.sequence, $start, $length )
- FROM dna d, contig c
- WHERE d.dna_id = c.dna_id
- AND c.contig_id = ?";
- }
-
- }
-
- #use the dna db if defined
- if( defined $self->db()->dnadb() ) {
- $sth = $self->db()->dnadb()->prepare( $query );
- } else {
- $sth = $self->prepare( $query );
- }
-
- $sth->execute($contig->dbID());
-
- if( my $aref = $sth->fetchrow_arrayref() ) {
- my ( $length, $seq, $n_line ) = @$aref;
- my $lenx=length($seq);
- if($compressed || $self->is_compressed()){
- $seq=$self->_dna_uncompress($length,$n_line,$start,$end,\$seq);
- }
- $seq =~ s/\s//g;
- if( $strand == -1 ) {
- return $self->_reverse_comp( $seq );
- } else {
- return $seq;
- }
- } else {
-
- my $flag;
- if($self->is_compressed==0){
- # found nothing...perhaps in this entry is compressed - check
- # and if present, set is_compressed
- eval{
- $query = "SELECT c.length, d.dna_id
- FROM dnac d, contig c
- WHERE d.dna_id = c.dna_id
- AND c.contig_id = ?";
- $sth = $self->prepare( $query );
- $sth->execute($contig->dbID());
- if( my $aref = $sth->fetchrow_arrayref() ) {
- $flag=1;
- }
- };
- if($flag==1){
- $self->is_compressed(1);
- print "Switched to compressed DNA reading mode\n";
- redo;
- }
- }else{
- # found nothing...perhaps in this entry is uncompressed - check
- # and if present, set is_compressed
- eval{
- $query = "SELECT c.length, d.dna_id
- FROM dna d, contig c
- WHERE d.dna_id = c.dna_id
- AND c.contig_id = ?";
- $sth = $self->prepare( $query );
- $sth->execute($contig->dbID());
- if( my $aref = $sth->fetchrow_arrayref() ) {
- $flag=1;
- }
- };
- if($flag==1){
- $self->is_compressed(0);
- print "Switched to uncompressed DNA reading mode\n";
- redo;
- }
- }
- return undef;
- }
- }
-}
-
-
-
-
=head2 fetch_by_Slice_start_end_strand
Arg [1] : Bio::EnsEMBL::Slice slice
The slice from which you want the sequence
- Arg [2] : int startBasePair
+ Arg [2] : (optional) int startBasePair
+ The start base pair relative to the start of the slice. Negative
+ values or values greater than the length of the slice are fine.
+ default = 1
+ Arg [3] : (optional) int endBasePair
+ The end base pair relative to the start of the slice. Negative
+ values or values greater than the length of the slice are fine,
+ but the end must be greater than or equal to the start
count from 1
- Arg [3] : int endBasePair
- count from 1, -1 is last one
- Arg [4] : int strand
+ default = the length of the slice
+ Arg [4] : (optional) int strand
1, -1
+ default = 1
Example : $dna = $seq_adptr->fetch_by_Slice_start_end_strand($slice, 1,
1000, -1);
Description: retrieves from db the sequence for this slice
@@ -229,392 +69,225 @@ sub fetch_by_RawContig_start_end_strand {
sub fetch_by_Slice_start_end_strand {
my ( $self, $slice, $start, $end, $strand ) = @_;
- my $seq= "";
-
- if( !$slice ){
- $self->throw("need a slice to work\n");
+ if(!ref($slice) || !$slice->isa("Bio::EnsEMBL::Slice")) {
+ throw("Slice argument is required.");
}
- unless
- ($slice->isa("Bio::EnsEMBL::Slice")) {
- $self->throw("$slice isn't a slice");
- }
-
- if( $end == -1 ) {
- $end = $slice->chr_end() - $slice->chr_start() + 1;
- }
+ $start = 1 if(!defined($start));
+
+ $end = $slice->end() - $slice->start() + 1 if(!defined($end));
+
+ throw("Start must be less than or equal to end.") if($start > $end);
+
+ $strand ||= 1;
+
+ #get a new slice that spans the exact region to retrieve dna from
+ my $right_expand = $end - $slice->length(); #negative is fine
+ my $left_expand = 1 - $start; #negative is fine
- # need to check the strand'edness of the slice as this
- # affects the direction in which the dna seq is grabbed
- if ( $slice->strand == 1 ) {
- $seq = $self->fetch_by_assembly_location
- (
- $slice->chr_start()+$start-1,
- $slice->chr_start()+$end-1,
- $strand,
- $slice->chr_name(),
- $slice->assembly_type()
- );
+ if($right_expand || $left_expand) {
+ $slice = $slice->expand($left_expand, $right_expand);
}
- elsif ( $slice->strand == -1 ) {
- $seq = $self->fetch_by_assembly_location
- (
- $slice->chr_end()-$end+1,
- $slice->chr_end()-$start+1,
- $strand * -1, #have to make strand relative to slice's strand
- $slice->chr_name(),
- $slice->assembly_type()
- );
+
+ #retrieve normalized 'non-symlinked' slices
+ #this allows us to support haplotypes and PARs
+ my $slice_adaptor = $slice->adaptor();
+ my @symproj=@{$slice_adaptor->fetch_normalized_slice_projection($slice)};
+
+ if(@symproj == 0) {
+ throw('Could not retrieve normalized Slices. Database contains ' .
+ 'incorrect assembly_exception information.');
}
- else {
- $self->throw("Incorrect strand set on slice $slice");
+
+ #call this method again with any slices that were 'symlinked' to by this
+ #slice
+ if(@symproj != 1 || $symproj[0]->[2] != $slice) {
+ my $seq;
+ foreach my $segment (@symproj) {
+ my $symlink_slice = $segment->[2];
+ #get sequence from each symlinked area
+ $seq .= ${$self->fetch_by_Slice_start_end_strand($symlink_slice,
+ 1,undef,1)};
+ }
+ if($strand == -1) {
+ _reverse_comp(\$seq);
+ }
+ return \$seq;
}
- return $seq;
-}
+ # we need to project this slice onto the sequence coordinate system
+ # even if the slice is in the same coord system, we want to trim out
+ # flanking gaps (if the slice is past the edges of the seqregion)
+ my $csa = $self->db->get_CoordSystemAdaptor();
+ my $seqlevel = $csa->fetch_sequence_level();
+ my @projection=@{$slice->project($seqlevel->name(), $seqlevel->version())};
-=head2 fetch_by_assembly_location
+ my $seq = '';
+ my $total = 0;
+ my $tmp_seq;
- Arg [1] : int $chrStart
- Arg [2] : int $chrEnd
- Arg [3] : int $strand
- Arg [4] : txt $chrName
- Arg [5] : txt $assemblyType
- Example : $dna = $fetch_by_assembly_location( 1, 100, -1, 'X', NCBI30 );
- Description: retrieve specified sequence from db. Using AssemblyMapper. Gaps
- are filled with N
- Returntype : string
- Exceptions : Wrong parameters give undef as result
- Caller : general, fetch_by_Slice_start_end_strand
+ #fetch sequence from each of the sequence regions projected onto
+ foreach my $segment (@projection) {
+ my ($start, $end, $seq_slice) = @$segment;
-=cut
+ #check for gaps between segments and pad them with Ns
+ my $gap = $start - $total - 1;
+ if($gap) {
+ $seq .= 'N' x $gap;
+ }
-sub fetch_by_assembly_location {
- my ( $self, $chrStart, $chrEnd,
- $strand, $chrName, $assemblyType ) = @_;
+ my $seq_region_id = $slice_adaptor->get_seq_region_id($seq_slice);
+
+ $tmp_seq = ${$self->_fetch_seq($seq_region_id,
+ $seq_slice->start, $seq_slice->length())};
- my $mapper =
- $self->db->get_AssemblyMapperAdaptor->fetch_by_type($assemblyType);
- # $mapper->register_region($chrName,$chrStart,$chrEnd);
-
- my @coord_list = $mapper->map_coordinates_to_rawcontig
- ( $chrName, $chrStart, $chrEnd, $strand );
-
- # for each of the pieces get sequence
- my $seq = "";
- for my $segment ( @coord_list ) {
- if( $segment->isa( "Bio::EnsEMBL::Mapper::Coordinate" )) {
-
- my $contig =
- $self->db->get_RawContigAdaptor()->fetch_by_dbID($segment->id());
-
- my $contig_seq = $self->fetch_by_RawContig_start_end_strand
- ( $contig,
- $segment->start(),
- $segment->end(),
- $segment->strand() );
-
- $seq .= $contig_seq;
-
- } else {
- # its a gap
- my $length = $segment->end() - $segment->start() + 1;
- $seq .= "N" x $length;
+ #reverse compliment on negatively oriented slices
+ if($seq_slice->strand == -1) {
+ _reverse_comp(\$tmp_seq);
}
+
+ $seq .= $tmp_seq;
+
+ $total = $end;
}
-
- return $seq;
+
+ #check for any remaining gaps at the end
+ my $gap = $slice->length - $total;
+ if($gap) {
+ $seq .= 'N' x $gap;
+ }
+
+ #if the sequence is too short it is because we came in with a seqlevel
+ #slice that was partially off of the seq_region. Pad the end with Ns
+ #to make long enough
+ if(length($seq) != $slice->length()) {
+ $seq .= 'N' x ($slice->length() - length($seq));
+ }
+
+ #if they asked for the negative slice strand revcomp the whole thing
+ _reverse_comp(\$seq) if($strand == -1);
+
+ return \$seq;
}
+sub _fetch_seq {
+ my $self = shift;
+ my $seq_region_id = shift;
+ my $start = shift;
+ my $length = shift;
+
+ my $tmp_seq;
+
+ my $sth = $self->prepare(
+ "SELECT SUBSTRING( d.sequence, ?, ?)
+ FROM dna d
+ WHERE d.seq_region_id = ?");
+ $sth->execute($start, $length, $seq_region_id);
+ $sth->bind_columns(\$tmp_seq);
+ $sth->fetch();
+ $sth->finish();
+
+ return \$tmp_seq;
+}
+
+
=head2 store
- Arg [1] : string $sequence the dna sequence to be stored in the database
- Arg [2] : string $date create date to be associated with the dna sequence
- to be stored.
- Example : $dbID = $seq_adaptor->store('ACTGGGTACCAAACAAACACAACA', $date);
+ Arg [1] : int $seq_region_id the id of the sequence region this dna
+ will be associated with.
+ Arg [2] : string $sequence the dna sequence to be stored
+ in the database. Note that the sequence passed in will be
+ converted to uppercase.
+ Example : $seq_adaptor->store(11, 'ACTGGGTACCAAACAAACACAACA');
Description: stores a dna sequence in the databases dna table and returns the
database identifier for the new record.
- Returntype : int
- Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::RawContigAdaptor::store
+ Returntype : none
+ Exceptions : throw if the database insert fails
+ Caller : sequence loading scripts
=cut
sub store {
- my ($self, $sequence, $date) = @_;
-
- $sequence =~ tr/atgcn/ATGCN/;
+ my ($self, $seq_region_id, $sequence) = @_;
- # save compressed
- if($self->is_compressed){
- return $self->store_compressed($sequence,$date);
+ if(!$seq_region_id) {
+ throw('seq_region_id is required');
}
-
- my $statement = $self->prepare("
- INSERT INTO dna(sequence,created)
- VALUES(?, FROM_UNIXTIME(?))
- ");
-
- my $rv = $statement->execute($sequence, $date);
- $self->throw("Failed to insert dna $sequence") unless $rv;
-
- $statement->finish;
-
- $statement = $self->prepare("SELECT last_insert_id()");
- $statement->execute();
- my ($id) = $statement->fetchrow();
- $statement->finish;
-
- return $id;
-}
+ $sequence = uc($sequence);
-=head2 store_compressed
+ my $statement =
+ $self->prepare("INSERT INTO dna(seq_region_id, sequence) VALUES(?,?)");
- Arg [1] : string $sequence the dna sequence to be stored in the database
- Arg [2] : string $date create date to be associated with the dna sequence
- to be stored.
- Arg [3] : dbid (optional)
- Returntype : int
- Exceptions : none
- Caller : Bio::EnsEMBL::DBSQL::RawContigAdaptor::store_compressed,
- Bio::EnsEMBL::DBSQL::RawContigAdaptor::store
+ $statement->execute($seq_region_id, $sequence);
-=cut
+ $statement->finish();
-sub store_compressed {
- my ($self, $sequence, $date, $dbid) = @_;
-
- # look for N's, save list of start and ends and convert to A's
- my $n_line;
- my $len=length($sequence);
- #print "DEBUG $sequence ($len)\n";
- {
- if($sequence=~/^([ACGT]*)([^ACGT]+)(.*)$/){
- my $l1=length($1);
- my $l2=length($2);
- my $start=$l1+1;
- my $end=$l1+$l2;
- $sequence=$1.('A' x $l2).$3;
- # might be more than one letter
- my $insert=$2;
- #print "DEBUG: $insert ($start,$end)\n";
- {
- if($insert=~/^(\w)(\1*)(.*)$/){
- my $insert2=$1.$2;
- my $end2=$start+length($insert2)-1;
- $n_line.=" " if $n_line;
- my $modifier='';
- if($1 ne 'N'){$modifier=":$1";}
- $n_line.="$start-$end2$modifier";
- #print "DEBUG: write $start-$end2$modifier\n";
- $insert=$3;
- $start=$end2+1;
- redo if $insert;
- }
- }
- redo;
- }
- }
- #print "DEBUG $sequence\n";
-
- # convert ACGT -> 00011011
- my $sequence_comp=$self->_dna_compress($sequence);
-
- my $id;
- if($dbid){
- my $statement = $self->prepare("
- INSERT INTO dnac(dna_id,sequence,created,n_line)
- VALUES(?, ?, FROM_UNIXTIME(?), ?)
- ");
-
- my $rv = $statement->execute($dbid,$sequence_comp, $date, $n_line);
- $self->throw("Failed to insert dna $sequence") unless $rv;
- $statement->finish;
- $id=$dbid;
- }else{
- my $statement = $self->prepare("
- INSERT INTO dnac(sequence,created,flag_dnan)
- VALUES(?, FROM_UNIXTIME(?), ?)
- ");
-
- my $rv = $statement->execute($sequence_comp, $date, $n_line);
- $self->throw("Failed to insert dna $sequence") unless $rv;
- $statement->finish;
-
- $statement = $self->prepare("SELECT last_insert_id()");
- $statement->execute();
- ($id) = $statement->fetchrow();
- $statement->finish;
- }
- return $id;
+ return;
}
-=head2 _dna_compress
+=head2 _reverse_comp
- Arg 1 : txt $dna_sequence
- Function : build hex representation of string (ACGT -> 1B)
- Returntype: txt
- Exceptions: none
- Caller : private to this module
+ Arg [1] : reference to a string $seqref
+ Example : $self->_reverse_comp(\$seqref);
+ Description: Does an in place reverse compliment of a passed in string
+ reference. The string is passed by reference
+ rather than by value for memory efficiency.
+ Returntype : none
+ Exceptions : none
+ Caller : internal
=cut
-{
-
- my %table=(
- 'AA'=>'0',
- 'AC'=>'1',
- 'AG'=>'2',
- 'AT'=>'3',
- 'CA'=>'4',
- 'CC'=>'5',
- 'CG'=>'6',
- 'CT'=>'7',
- 'GA'=>'8',
- 'GC'=>'9',
- 'GG'=>'A',
- 'GT'=>'B',
- 'TA'=>'C',
- 'TC'=>'D',
- 'TG'=>'E',
- 'TT'=>'F',
- );
-
- sub _dna_compress {
- my $self = shift;
- my $seq = shift;
- # length must be multiple of 4, so pad if necesary
- my $len=length($seq);
- $seq.='A' x ($len-(int($len/4))*4);
- #print "DEBUG $seq\n";
- # process in blocks of 2 to do conversion
- $seq=~s/(\G..)/$table{$1}/g;
- return pack("H*",$seq);
- }
-}
+sub _reverse_comp {
+ my $seqref = shift;
+ $$seqref = reverse( $$seqref );
+ $$seqref =~
+ tr/acgtrymkswhbvdnxACGTRYMKSWHBVDNX/tgcayrkmswdvbhnxTGCAYRKMSWDVBHNX/;
-=head2 _dna_uncompress
+ return undef;
+}
- Arg [1] : int $length
- Arg [2] : int $n_line
- Arg [3] : int $start
- Arg [4] : int $end
- Arg [5] : reference to $seq (hex string)
- Function : converts hex string from DB into ACGT with appropriate truncation
- Returntype : string
- Exceptions : none
- Caller : private to this module
-=cut
-{
-
- my %table=(
- '0'=>'AA',
- '1'=>'AC',
- '2'=>'AG',
- '3'=>'AT',
- '4'=>'CA',
- '5'=>'CC',
- '6'=>'CG',
- '7'=>'CT',
- '8'=>'GA',
- '9'=>'GC',
- 'A'=>'GG',
- 'B'=>'GT',
- 'C'=>'TA',
- 'D'=>'TC',
- 'E'=>'TG',
- 'F'=>'TT',
- );
-
- sub _dna_uncompress {
- my($self,$length,$n_line,$start,$end,$rseq)=@_;
-
- # convert sequence back to ACGT (already in Hex)
- my $seq2=join('',map{$table{$_}}split(//,$$rseq));
-
- # calculate extent of $seq2 and truncate
- my $start4=(int(($start-1)/4)+1)*4-3;
- my $off=$start-$start4;
- my $len;
- if($end==-1){
- $end=$length;
- }
- $len=$end-$start+1;
- #print "truncate: $start,$start4,$end,$len\n";
- my $seq=substr($seq2,$off,$len);
-
- # mask with N's
- foreach my $range (split(/ /,$n_line)){
- my($st,$ed)=split(/\-/,$range);
- my $char='N';
- if($ed=~/(\d+):(\w)/){
- $ed=$1;
- $char=$2;
- }
- #print "before: $st-$ed $start-$end\n";
- # check in range
- next if($ed<$start || $st>$end);
- $ed=$end if $ed>$end;
- $st=$start if $st<$start;
- #print "after: $st-$ed\n";
- my $len=$ed-$st+1;
- $st-=$start;
- substr($seq,$st,$len)=$char x $len;
- }
-
- return $seq;
- }
-}
+=head2 fetch_by_assembly_location
+ Description: DEPRECATED use fetch_by_Slice_start_end_strand() instead.
-=head2 is_compressed
+=cut
- Arg [1] : (optional) flag
- to set or unset
- Example : $adaptor->is_compressed(1)
- Description: Getter/Setter for whether compressed DNA should be read/written
- Returntype : true/false
- Exceptions : none
- Caller : Adaptors inherited from BaseAdaptor
+sub fetch_by_assembly_location {
+ my ( $self, $chrStart, $chrEnd,
+ $strand, $chrName, $assemblyType ) = @_;
-=cut
+ deprecate('Use fetch_by_Slice_start_end_strand() instead');
+
+ my $slice_adaptor = $self->db->get_SliceAdaptor();
+ my $slice = $slice_adaptor->fetch_by_region('toplevel', $chrName,
+ $chrStart, $chrEnd,
+ $strand);
-sub is_compressed {
- my( $obj, $flag ) = @_;
- if (defined $flag) {
- $obj->{'_is_truncated'} = $flag ? 1 : 0;
- }
- return $obj->{'_is_truncated'};
+ return $self->fetch_by_Slice_start_end_strand($slice,1, $slice->length,1);
}
-=head2 _reverse_comp
+=head2 fetch_by_RawContig_start_end_strand
- Arg 1 : txt $dna_sequence
- Function : build reverse complement string
- Returntype: txt
- Exceptions: none
- Caller : private to this module
+ Description: DEPRECATED use fetch_by_Slice_start_end_strand instead
=cut
-sub _reverse_comp {
- my $self = shift;
- my $seq = shift;
-
- $seq = reverse( $seq );
- $seq =~
- tr/acgtrymkswhbvdnxACGTRYMKSWHBVDNX/tgcayrkmswdvbhnxTGCAYRKMSWDVBHNX/;
- return $seq;
+sub fetch_by_RawContig_start_end_strand {
+ deprecate('Use fetch_by_Slice_start_end_strand instead.');
+ fetch_by_Slice_start_end_strand(@_);
}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/SimpleFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/SimpleFeatureAdaptor.pm
index fdc46d8533..69ce3e6fe9 100644
--- a/modules/Bio/EnsEMBL/DBSQL/SimpleFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/SimpleFeatureAdaptor.pm
@@ -1,7 +1,6 @@
#
# EnsEMBL module for Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor
#
-# Cared for by Ewan Birney <birney@ebi.ac.uk>
#
# Copyright EMBL/EBI
#
@@ -11,38 +10,30 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor
+Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor
=head1 SYNOPSIS
my $simple_feature_adaptor = $database_adaptor->get_SimpleFeatureAdaptor();
-@simple_features = $simple_feature_adaptor->fetch_by_Slice($slice);
+@simple_features = @{$simple_feature_adaptor->fetch_all_by_Slice($slice)};
=head1 DESCRIPTION
-Simple Feature Adaptor - database access for simple features
+Simple Feature Adaptor - database access for simple features
=head1 AUTHOR - Ewan Birney
-Email birney@ebi.ac.uk
-
-=head1 APPENDIX
-
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-
-# Let the code begin...
-
-
package Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor;
use vars qw(@ISA);
use strict;
use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
use Bio::EnsEMBL::SimpleFeature;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
@@ -55,7 +46,7 @@ use Bio::EnsEMBL::SimpleFeature;
Description: Stores a list of simple feature objects in the database
Returntype : none
Exceptions : thrown if @sf is not defined, if any of the features do not
- have an attached contig object,
+ have an attached slice.
or if any elements of @sf are not Bio::EnsEMBL::SeqFeatures
Caller : general
@@ -63,45 +54,52 @@ use Bio::EnsEMBL::SimpleFeature;
sub store{
my ($self,@sf) = @_;
-
+
if( scalar(@sf) == 0 ) {
- $self->throw("Must call store with list of sequence features");
+ throw("Must call store with list of SimpleFeatures");
}
-
- my $sth =
- $self->prepare("INSERT INTO simple_feature (contig_id, contig_start,
- contig_end, contig_strand,
- display_label, analysis_id,
- score)
- VALUES (?,?,?,?,?,?,?)");
-
- foreach my $sf ( @sf ) {
+
+ my $sth = $self->prepare
+ ("INSERT INTO simple_feature (seq_region_id, seq_region_start, " .
+ "seq_region_end, seq_region_strand, " .
+ "display_label, analysis_id, score) " .
+ "VALUES (?,?,?,?,?,?,?)");
+
+ my $db = $self->db();
+ my $analysis_adaptor = $db->get_AnalysisAdaptor();
+
+ FEATURE: foreach my $sf ( @sf ) {
+
if( !ref $sf || !$sf->isa("Bio::EnsEMBL::SimpleFeature") ) {
- $self->throw("Simple feature must be an Ensembl SimpleFeature, " .
- "not a [$sf]");
+ throw("SimpleFeature must be an Ensembl SimpleFeature, " .
+ "not a [".ref($sf)."]");
}
-
- if( !defined $sf->analysis ) {
- $self->throw("Cannot store sequence features without analysis");
+
+ if($sf->is_stored($db)) {
+ warning("SimpleFeature [".$sf->dbID."] is already stored" .
+ " in this database.");
+ next FEATURE;
}
- if( !defined $sf->analysis->dbID ) {
- $self->throw("I think we should always have an analysis object " .
- "which has originated from the database. No dbID, " .
- "not putting in!");
+
+ if(!defined($sf->analysis)) {
+ throw("An analysis must be attached to the features to be stored.");
}
-
- my $contig = $sf->entire_seq();
- unless(defined $contig && $contig->isa("Bio::EnsEMBL::RawContig")) {
- $self->throw("Cannot store feature without a Contig object attached via "
- . "attach_seq\n");
+
+ #store the analysis if it has not been stored yet
+ if(!$sf->analysis->is_stored($db)) {
+ $analysis_adaptor->store($sf->analysis());
}
- # store analysis if not there already
- $self->db->get_AnalysisAdaptor->store($sf->analysis);
+ my $original = $sf;
+ my $seq_region_id;
+ ($sf, $seq_region_id) = $self->_pre_store($sf);
- $sth->execute($contig->dbID(), $sf->start, $sf->end, $sf->strand,
- $sf->display_label, $sf->analysis->dbID, $sf->score);
- }
+ $sth->execute($seq_region_id, $sf->start, $sf->end, $sf->strand,
+ $sf->display_label, $sf->analysis->dbID, $sf->score);
+
+ $original->dbID($sth->{'mysql_insertid'});
+ $original->adaptor($self);
+ }
}
@@ -139,9 +137,9 @@ sub _tables {
sub _columns {
my $self = shift;
- return qw( sf.simple_feature_id
- sf.contig_id sf.contig_start sf.contig_end sf.contig_strand
- sf.display_label sf.analysis_id score );
+ return qw( sf.simple_feature_id
+ sf.seq_region_id sf.seq_region_start sf.seq_region_end
+ sf.seq_region_strand sf.display_label sf.analysis_id sf.score );
}
@@ -158,33 +156,133 @@ sub _columns {
=cut
sub _objs_from_sth {
- my ($self, $sth) = @_;
+ my ($self, $sth, $mapper, $dest_slice) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
+
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $aa = $self->db->get_AnalysisAdaptor();
+
+ my @features;
+ my %analysis_hash;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+
+ my($simple_feature_id,$seq_region_id, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $display_label, $analysis_id, $score);
+
+ $sth->bind_columns(\$simple_feature_id,\$seq_region_id, \$seq_region_start,
+ \$seq_region_end, \$seq_region_strand, \$display_label,
+ \$analysis_id, \$score);
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
- my $aa = $self->db()->get_AnalysisAdaptor();
- my $rca = $self->db()->get_RawContigAdaptor();
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
- my @features = ();
-
- my $hashref;
- while($hashref = $sth->fetchrow_hashref()) {
- my $contig = $rca->fetch_by_dbID($hashref->{'contig_id'});
- my $analysis = $aa->fetch_by_dbID($hashref->{'analysis_id'});
-
- my $out = Bio::EnsEMBL::SimpleFeature->new();
- $out->start($hashref->{'contig_start'});
- $out->end($hashref->{'contig_end'});
- $out->strand($hashref->{'contig_strand'});
- $out->analysis($analysis);
- $out->display_label($hashref->{'display_label'});
- $out->attach_seq($contig);
-
- if($hashref->{'score'}) {
- $out->score($hashref->{'score'});
+ FEATURE: while($sth->fetch()) {
+ #get the analysis object
+ my $analysis = $analysis_hash{$analysis_id} ||=
+ $aa->fetch_by_dbID($analysis_id);
+
+ #get the slice object
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+ $slice = $dest_slice;
}
-
- $out->dbID($hashref->{'simple_feature_id'});
- push @features, $out;
+ push @features, Bio::EnsEMBL::SimpleFeature->new_fast(
+ {'start' => $seq_region_start,
+ 'end' => $seq_region_end,
+ 'strand' => $seq_region_strand,
+ 'slice' => $slice,
+ 'analysis' => $analysis,
+ 'adaptor' => $self,
+ 'dbID' => $simple_feature_id,
+ 'display_label' => $display_label,
+ 'score' => $score});
}
return \@features;
diff --git a/modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm
index 0dfd888126..bf99509e44 100644
--- a/modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/SliceAdaptor.pm
@@ -12,66 +12,103 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::SliceAdaptor - Adaptors for slices
+Bio::EnsEMBL::DBSQL::SliceAdaptor - A database aware adaptor responsible for
+the creation of Slice objects.
=head1 SYNOPSIS
-
+ my $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+
+ my $slice_adaptor = $db->get_SliceAdaptor();
+
+ #get a slice on the entire chromosome X
+ my $chr_slice = $slice_adaptor->fetch_by_region('chromosome','X');
+
+ #get a slice for each clone in the database
+ foreach my $cln_slice (@{$slice_adaptor->fetch_all('clone')}) {
+ #do something with clone
+ }
+
+ #get a slice which is part of NT_004321
+ my $spctg_slice = $slice_adaptor->fetch_by_region('supercontig','NT_004321',
+ 200_000, 600_000);
=head1 DESCRIPTION
-Factory for getting out slices of assemblies. WebSlice is the highly
-accelerated version for the web site.
+This module is responsible for fetching Slices representing genomic regions
+from a database. Details on how slices can be used are in the
+Bio::EnsEMBL::Slice module.
=head1 AUTHOR - Ewan Birney
This modules is part of the Ensembl project http://www.ensembl.org
-Email ensembl-dev@ebi.ac.uk
-
-Describe contact details here
+=head1 CONTACT
-=head1 APPENDIX
+Email ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object
-methods. Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-# Let the code begin...
-
-
package Bio::EnsEMBL::DBSQL::SliceAdaptor;
use vars qw(@ISA);
use strict;
-# Object preamble - inherits from Bio::EnsEMBL::Root
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
use Bio::EnsEMBL::Slice;
use Bio::EnsEMBL::DBSQL::DBAdaptor;
+use Bio::EnsEMBL::Mapper;
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate warning);
+use Bio::EnsEMBL::Utils::Cache; #CPAN LRU cache
@ISA = ('Bio::EnsEMBL::DBSQL::BaseAdaptor');
+my $SEQ_REGION_CACHE_SIZE = 100;
-# new is inherited from BaseAdaptor
+sub new {
+ my $caller = shift;
+ my $class = ref($caller) || $caller;
+ my $self = $class->SUPER::new(@_);
-=head2 fetch_by_chr_start_end
+ my %name_cache;
+ my %id_cache;
- Arg [1] : string $chr
- the name of the chromosome to obtain a slice for
- Arg [2] : int $start
- the start basepair of the slice to obtain in chromosomal
- coordinates
- Arg [3] : int $end
- the end basepair of the slice to obtain in chromosomal
- coordinates
- Example : $slice = $slice_adaptor->fetch_by_chr_start_end();
+ tie(%name_cache, 'Bio::EnsEMBL::Utils::Cache', $SEQ_REGION_CACHE_SIZE);
+ tie(%id_cache, 'Bio::EnsEMBL::Utils::Cache', $SEQ_REGION_CACHE_SIZE);
+
+ $self->{'_name_cache'} = \%name_cache;
+ $self->{'_id_cache'} = \%id_cache;
+
+ return $self;
+}
+
+
+=head2 fetch_by_region
+
+ Arg [1] : string $coord_system_name
+ The name of the coordinate system of the slice to be created
+ This may be a name of an acutal coordinate system or an alias
+ to a coordinate system. Valid aliases are 'seqlevel' or
+ 'toplevel'.
+ Arg [2] : string $seq_region_name
+ The name of the sequence region that the slice will be
+ created on
+ Arg [3] : int $start (optional, default = 1)
+ The start of the slice on the sequence region
+ Arg [4] : int $end (optional, default = seq_region length)
+ The end of the slice on the sequence region
+ Arg [5] : int $strand (optional, default = 1)
+ The orientation of the slice on the sequence region
+ Arg [6] : string $version (optional, default = default version)
+ The version of the coordinate system to use (e.g. NCBI33)
+ Example : $slice = $slice_adaptor->fetch_by_region('chromosome');
Description: Creates a slice object on the given chromosome and coordinates.
Returntype : Bio::EnsEMBL::Slice
Exceptions : none
@@ -79,275 +116,530 @@ use Bio::EnsEMBL::DBSQL::DBAdaptor;
=cut
-sub fetch_by_chr_start_end {
- my ($self,$chr,$start,$end) = @_;
-
- unless($chr) {
- $self->throw("chromosome name argument must be defined and not ''");
+sub fetch_by_region {
+ my ($self, $coord_system_name, $seq_region_name,
+ $start, $end, $strand, $version) = @_;
+
+ throw('seq_region_name argument is required') if(!$seq_region_name);
+
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $coord_system = $csa->fetch_by_name($coord_system_name,$version);
+
+ #check the cache so we only go to the db if necessary
+ my $name_cache = $self->{'_name_cache'};
+ my $key = lc(join(':',$seq_region_name,
+ $coord_system->name(),
+ $coord_system->version));
+
+ my $length;
+
+ if(exists($name_cache->{$key})) {
+ $length = $name_cache->{$key}->[1];
+ } else {
+ my $sth = $self->prepare("SELECT seq_region_id, length " .
+ "FROM seq_region " .
+ "WHERE name = ? AND coord_system_id = ?");
+
+ #force seq_region_name cast to string so mysql cannot treat as int
+ $sth->execute("$seq_region_name", $coord_system->dbID());
+
+ if($sth->rows() != 1) {
+ $version ||= '';
+ $seq_region_name ||= '';
+ $coord_system_name ||= '';
+ throw("Cannot create slice on non-existant or ambigous seq_region:\n" .
+ " coord_system=[$coord_system_name],\n" .
+ " name=[$seq_region_name],\n" .
+ " version=[$version]");
}
- unless(defined $end) { # Why defined? Is '0' a valid end?
- $self->throw("end argument must be defined\n");
- }
+ my $id;
+ ($id, $length) = $sth->fetchrow_array();
+ $sth->finish();
- unless(defined $start) {
- $self->throw("start argument must be defined\n");
- }
+ #cache results to speed up future queries
+ $name_cache->{$key} = [$id,$length];
+ $self->{'_id_cache'}->{$id} = [$seq_region_name, $length, $coord_system];
+ }
- if($start > $end) {
- $self->throw("start must be less than end: parameters $chr:$start:$end");
- }
-
- my $slice;
- my $type = $self->db->assembly_type();
-
- $slice = Bio::EnsEMBL::Slice->new(
- -chr_name => $chr,
- -chr_start => $start,
- -chr_end => $end,
- -assembly_type => $type,
- -adaptor => $self
- );
-
- return $slice;
+ $start = 1 if(!defined($start));
+ $strand = 1 if(!defined($strand));
+ $end = $length if(!defined($end));
+
+ if($end < $start) {
+ throw('start [$start] must be less than or equal to end [$end]');
+ }
+
+ return Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $coord_system,
+ -SEQ_REGION_NAME => $seq_region_name,
+ -START => $start,
+ -END => $end,
+ -STRAND => $strand,
+ -ADAPTOR => $self);
}
-=head2 fetch_by_contig_name
+=head2 fetch_by_name
Arg [1] : string $name
- the name of the contig to obtain a slice for
- Arg [2] : (optional) int $size
- the size of the flanking regions to obtain (aka context size)
- Example : $slc = $slc_adaptor->fetch_by_contig_name('AB000878.1.1.33983');
- Description: Creates a slice object around the specified contig.
- If a context size is given, the slice is extended by that
- number of basepairs on either side of the contig.
+ Example : $name = 'chromosome:NCBI34:X:1000000:2000000:1';
+ $slice = $slice_adaptor->fetch_by_name($name);
+ $slice2 = $slice_adaptor->fetch_by_name($slice3->name());
+ Description: Fetches a slice using a slice name (i.e. the value returned by
+ the Slice::name method). This is useful if you wish to
+ store a unique identifier for a slice in a file or database or
+ pass a slice over a network.
+ Slice::name allows you to serialise/marshall a slice and this
+ method allows you to deserialise/unmarshal it.
Returntype : Bio::EnsEMBL::Slice
- Exceptions : none
- Caller : general
+ Exceptions : throw if incorrent arg provided
+ Caller : Pipeline
=cut
-sub fetch_by_contig_name {
- my ($self,$name, $size) = @_;
-
- if( !defined $size ) {$size=0;}
+sub fetch_by_name {
+ my $self = shift;
+ my $name = shift;
- my ($chr_name,$start,$end) = $self->_get_chr_start_end_of_contig($name);
-
- $start -= $size;
- $end += $size;
-
- if($start < 1) {
- $start = 1;
+ if(!$name) {
+ throw("name argument is required");
}
-
- return $self->fetch_by_chr_start_end($chr_name, $start, $end);
+
+ my @array = split(/:/,$name);
+
+ if(@array != 6) {
+ throw("Malformed slice name [$name]. Format is " .
+ "coord_system:version:start:end:strand");
+ }
+
+ my ($cs_name, $cs_version, $seq_region, $start, $end, $strand) = @array;
+
+
+ return $self->fetch_by_region($cs_name,$seq_region, $start,
+ $end, $strand, $cs_version);
}
-=head2 fetch_by_supercontig_name
- Arg [1] : string $supercontig_name
- Example : $slice = $slice_adaptor->fetch_by_supercontig_name('NT_004321');
- Description: Creates a Slice on the region of the assembly where
- the specified super contig lies. Note that this slice will
- have the same orientation as the supercontig. If the supercontig
- has a negative assembly orientation, the slice will also have
- a negative orientation relative to the assembly.
+=head2 fetch_by_seq_region_id
+
+ Arg [1] : string $seq_region_id
+ The internal identifier of the seq_region to create this slice
+ on
+ Example : $slice = $slice_adaptor->fetch_by_seq_region_id(34413);
+ Description: Creates a slice object of an entire seq_region using the
+ seq_region internal identifier to resolve the seq_region.
Returntype : Bio::EnsEMBL::Slice
Exceptions : none
Caller : general
=cut
-sub fetch_by_supercontig_name {
- my ($self,$supercontig_name) = @_;
-
- my $assembly_type = $self->db->assembly_type();
-
- my $sth = $self->db->prepare(
- "SELECT chr.name, a.superctg_ori, MIN(a.chr_start), MAX(a.chr_end)
- FROM assembly a, chromosome chr
- WHERE superctg_name = ? AND type = ? AND chr.chromosome_id = a.chromosome_id
- GROUP BY superctg_name"
- );
-
- $sth->execute( $supercontig_name, $assembly_type );
-
- my ($chr, $strand, $slice_start, $slice_end) = $sth->fetchrow_array;
-
- my $slice;
-
- $slice = new Bio::EnsEMBL::Slice
- (
- -chr_name => $chr,
- -chr_start =>$slice_start,
- -chr_end => $slice_end,
- -strand => $strand,
- -assembly_type => $assembly_type,
- -adaptor => $self
- );
-
- return $slice;
+sub fetch_by_seq_region_id {
+ my ($self, $seq_region_id) = @_;
+
+ my $id_cache = $self->{'_id_cache'};
+
+ my ($name, $length, $cs);
+
+ if(exists $id_cache->{$seq_region_id}) {
+ ($name, $length, $cs) = @{$id_cache->{$seq_region_id}};
+ } else {
+ my $sth = $self->prepare("SELECT name, length, coord_system_id " .
+ "FROM seq_region " .
+ "WHERE seq_region_id = ?");
+
+ $sth->execute($seq_region_id);
+
+ if($sth->rows() != 1) {
+ throw("Cannot create slice on non-existant or ambigous seq_region:" .
+ " seq_region_id=[$seq_region_id],\n");
+ }
+
+ my $cs_id;
+ ($name, $length, $cs_id) = $sth->fetchrow_array();
+ $sth->finish();
+
+ $cs = $self->db->get_CoordSystemAdaptor->fetch_by_dbID($cs_id);
+
+ #cache results to speed up repeated queries
+ $id_cache->{$seq_region_id} = [$name, $length, $cs];
+ my $key = lc(join(':', $name, $cs->name, $cs->version));
+ $self->{'_name_cache'}->{$key} = [$seq_region_id, $length];
+ }
+
+ return Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $cs,
+ -SEQ_REGION_NAME => $name,
+ -START => 1,
+ -END => $length,
+ -STRAND => 1,
+ -ADAPTOR => $self);
}
-=head2 list_overlapping_supercontigs
+
+=head2 get_seq_region_id
Arg [1] : Bio::EnsEMBL::Slice $slice
- overlapping given Sice
- Example :
- Description: return the names of the supercontigs that overlap given Slice.
- Returntype : listref string
- Exceptions : none
- Caller : general
+ The slice to fetch a seq_region_id for
+ Example : $srid = $slice_adaptor->get_seq_region_id($slice);
+ Description: Retrieves the seq_region id (in this database) given a slice
+ Seq region ids are not stored on the slices themselves
+ because they are intended to be somewhat database independant
+ and seq_region_ids vary accross databases.
+ Returntype : int
+ Exceptions : throw if the seq_region of the slice is not in the db
+ throw if incorrect arg provided
+ Caller : BaseFeatureAdaptor
=cut
+sub get_seq_region_id {
+ my $self = shift;
+ my $slice = shift;
-sub list_overlapping_supercontigs {
- my ($self,$slice) = @_;
- my $sth = $self->db->prepare( "
- SELECT DISTINCT superctg_name
- FROM assembly a, chromosome c
- WHERE c.chromosome_id = a.chromosome_id
- AND c.name = ?
- AND a.type = ?
- AND a.chr_end >= ?
- AND a.chr_start <= ?
- " );
- $sth->execute( $slice->chr_name(), $slice->assembly_type(),
- $slice->chr_start(), $slice->chr_end() );
-
- my $result = [];
- while( my $aref = $sth->fetchrow_arrayref() ) {
- push( @$result, $aref->[0] );
- }
+ if(!$slice || !ref($slice) || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw('Slice argument is required');
+ }
+
+ my $cs_name = $slice->coord_system->name();
+ my $cs_version = $slice->coord_system->version();
+ my $seq_region_name = $slice->seq_region_name();
+
+ my $key = lc(join(':', $seq_region_name,$cs_name,$cs_version));
- return $result;
+ my $name_cache = $self->{'_name_cache'};
+
+ if(exists($name_cache->{$key})) {
+ return $name_cache->{$key}->[0];
+ }
+
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $coord_system = $csa->fetch_by_name($cs_name,$cs_version);
+
+ my $sth = $self->prepare("SELECT seq_region_id, length " .
+ "FROM seq_region " .
+ "WHERE name = ? AND coord_system_id = ?");
+
+ #force seq_region_name cast to string so mysql cannot treat as int
+ $sth->execute("$seq_region_name", $coord_system->dbID());
+
+ if($sth->rows() != 1) {
+ throw("Non existant or ambigous seq_region:\n" .
+ " coord_system=[$cs_name],\n" .
+ " name=[$seq_region_name],\n" .
+ " version=[$cs_version]");
+ }
+
+ my($seq_region_id, $length) = $sth->fetchrow_array();
+ $sth->finish();
+
+ #cache information for future requests
+ $name_cache->{$key} = [$seq_region_id, $length];
+ $self->{'_id_cache'}->{$seq_region_id} =
+ [$seq_region_name, $length, $coord_system];
+
+ return $seq_region_id;
}
-=head2 fetch_by_chr_band
+=head2 get_seq_region_attribs
- Title : fetch_by_chr_band
- Usage :
- Function: create a Slice representing a series of bands
- Example :
- Returns :
- Args : the band name
+ Arg [1] : Bio::EnsEMBL::Slice $slice
+ The slice to fetch attributes for
+ Example : %attribs = %{$slice_adaptor->get_seq_region_attribs($slice)};
+ ($htg_phase) = @{$attribs->{'htg_phase'} || []};
+ @synonyms = @{$attribs->{'synonym'} || []};
+ Description: Retrieves a reference to a hash containing attrib code values
+ and listref value keys.
+ Returntype : hashref
+ Exceptions : throw if the seq_region of the slice is not in the db
+ throw if incorrect arg provided
+ Caller : Bio::EnsEMBL::Slice
+
+=cut
+
+sub get_seq_region_attribs {
+ my $self = shift;
+ my $slice = shift;
+
+ if(!ref($slice) || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw('Slice argument is required');
+ }
+
+ my $srid = $self->get_seq_region_id($slice);
+ if(!$srid) {
+ throw('Slice is not on a seq_region stored in this database.');
+ }
+
+ $self->{'_attribs_cache'} ||= {};
+ if($self->{'_attribs_cache'}->{$srid}) {
+ return $self->{'_attribs_cache'}->{$srid};
+ }
+
+ my $sth = $self->prepare('SELECT at.code, sra.value ' .
+ 'FROM seq_region_attrib sra, attrib_type at ' .
+ 'WHERE sra.seq_region_id = ? ' .
+ 'AND at.attrib_type_id = sra.attrib_type_id');
+
+ $sth->execute($srid);
+
+ my($code, $attrib);
+ $sth->bind_columns(\$code, \$attrib);
+
+ my %attrib_hash;
+ while($sth->fetch()) {
+ $attrib_hash{$code} ||= [];
+ push @{$attrib_hash{$code}}, $attrib;
+ }
+
+ $sth->finish();
+ $self->{'_attribs_cache'} = \%attrib_hash;
+ return \%attrib_hash;
+}
+
+
+=head2 fetch_all
+
+ Arg [1] : string $coord_system_name
+ The name of the coordinate system to retrieve slices of.
+ This may be a name of an acutal coordinate system or an alias
+ to a coordinate system. Valid aliases are 'seqlevel' or
+ 'toplevel'.
+ Arg [2] : string $coord_system_version (optional)
+ The version of the coordinate system to retrieve slices of
+ Arg [3] : int $max_length (optional)
+ The maximum length of slices to be returned. Seq_regions which
+ are larger than $max_length will be split into multiple slices.
+ If this argument is not provided then slices will not be
+ split. If this argument is less than 1 a warning will occur and
+ the slices will not be split.
+ Arg [4] : int $overlap (optional)
+ The amount that the returned slices should overlap. By default
+ this value is 0. If no max_length value is provided but an
+ overlap which is not zero is provided this argument will be
+ ignored and a warning will occur.
+ Example : @chromos = @{$slice_adaptor->fetch_all('chromosome','NCBI33')};
+ @contigs = @{$slice_adaptor->fetch_all('contig')};
+
+ #create chunks of size 100k with 10k overlap
+ @fixed_chunks = @{$slice_adaptor->fetch_all('chromosome', undef,
+ 1e5, 1e4);
+
+ Description: Retrieves slices of all seq_regions for a given coordinate
+ system. This is analagous to the methods fetch_all which were
+ formerly on the ChromosomeAdaptor, RawContigAdaptor and
+ CloneAdaptor classes. Slices fetched span the entire
+ seq_regions and are on the forward strand.
+ Returntype : listref of Bio::EnsEMBL::Slices
+ Exceptions : throw if invalid coord system is provided
+ throw if max_length < 1 is provided
+ throw if overlap < 0 is provided
+ throw if overlap is provided but max_length is not
+ throw if overlap is greater than max_length
+ Caller : general
=cut
-sub fetch_by_chr_band {
- my ($self,$chr,$band) = @_;
-
- my $type = $self->db->assembly_type();
-
- warn( "XX>" ,$chr, "--", $band );
- my $sth = $self->db->prepare("
- select min(k.chr_start), max(k.chr_end)
- from chromosome as c, karyotype as k
- where c.chromosome_id = k.chromosome_id and c.name=? and k.band like ?
- ");
- $sth->execute( $chr, "$band%" );
- my ( $slice_start, $slice_end) = $sth->fetchrow_array;
-
- warn( $chr, "--", $band );
- unless( defined($slice_start) ) {
- my $sth = $self->db->prepare("
- select min(k.chr_start), max(k.chr_end)
- from chromosome as c, karyotype as k
- where c.chromosome_id = k.chromosome_id and k.band like ?
- ");
- $sth->execute( "$band%" );
- ( $slice_start, $slice_end) = $sth->fetchrow_array;
+sub fetch_all {
+ my $self = shift;
+ my $cs_name = shift;
+ my $cs_version = shift || '';
+
+ my ($max_length, $overlap) = @_;
+
+ #
+ # sanity checking of the overlap / maxlength arguments
+ #
+ if(defined($max_length) && $max_length < 1) {
+ throw("Invalid max length [$max_length] provided.");
+ }
+
+ $overlap ||= 0;
+ if($overlap != 0) {
+ if(!defined($max_length)) {
+ throw("Cannot define overlap without defining valid max_length.");
}
+ if($overlap < 0) {
+ throw("Cannot define overlap that is less than 0.");
+ }
+ if($max_length <= $overlap) {
+ throw("Overlap must be less than max_length.");
+ }
+ }
- if(defined $slice_start) {
- return new Bio::EnsEMBL::Slice(
- -chr_name => $chr,
- -chr_start => $slice_start,
- -chr_end => $slice_end,
- -strand => 1,
- -assembly_type => $type
- );
+ #
+ # verify existance of requested coord system and get its id
+ #
+ my $csa = $self->db->get_CoordSystemAdaptor();
+ my $cs = $csa->fetch_by_name($cs_name, $cs_version);
+
+
+ #
+ # Retrieve the seq_region from the database
+ #
+ my $sth = $self->prepare('SELECT seq_region_id, name, length ' .
+ 'FROM seq_region ' .
+ 'WHERE coord_system_id =?');
+
+ $sth->execute($cs->dbID);
+
+ my ($seq_region_id, $name, $length);
+ $sth->bind_columns(\$seq_region_id, \$name, \$length);
+
+ my $name_cache = $self->{'_name_cache'};
+ my $id_cache = $self->{'_id_cache'};
+
+ my $cs_key = lc($cs->name().':'.$cs_version);
+
+ my @out;
+ while($sth->fetch()) {
+ #cache values for future reference
+ my $key = lc($name) . ':'. $cs_key;
+ $name_cache->{$key} = [$seq_region_id, $length];
+ $id_cache->{$seq_region_id} = [$name, $length, $cs];
+
+ #
+ # split the seq regions into appropriately sized chunks
+ #
+ my $start = 1;
+ my $end;
+ my $multiple;
+ my $number;
+
+ if($max_length && ($length > $overlap)) {
+ #No seq region may be longer than max_length but we want to make
+ #them all similar size so that the last one isn't much shorter.
+ #Divide the seq_region into the largest equal pieces that are shorter
+ #than max_length
+
+ #calculate number of slices to create
+ $number = ($length-$overlap) / ($max_length-$overlap);
+ $number = int($number + 1.0); #round up to int (ceiling)
+
+ #calculate length of created slices
+ $multiple = $length / $number;
+ $multiple = int($multiple); #round down to int (floor)
+ } else {
+ #just one slice of the whole seq_region
+ $number = 1;
+ $multiple = $length;
+ }
+
+ my $i;
+ for(my $i=0; $i < $number; $i++) {
+ $end = $start + $multiple + $overlap;
+
+ #any remainder gets added to the last slice of the seq_region
+ $end = $length if($i == $number-1);
+
+ push @out, Bio::EnsEMBL::Slice->new(-START => $start,
+ -END => $end,
+ -STRAND => 1,
+ -SEQ_REGION_NAME => $name,
+ -COORD_SYSTEM => $cs,
+ -ADAPTOR => $self);
+ $start += $multiple;
}
+ }
- $self->throw("Band not recognised in database");
+ return \@out;
}
-=head2 fetch_by_clone_accession
+=head2 fetch_all_non_redundant
- Arg [1] : string $clone
- the embl accession of the clone object to retrieve
- Arg [2] : (optional) int $size
- the size of the flanking regions to obtain around the clone
- Example : $slc = $slc_adaptor->fetch_by_clone_accession('AC000012',1000);
- Description: Creates a Slice around the specified clone. If a context size
- is given, the Slice is extended by that number of basepairs on
- either side of the clone. Throws if the clone is not golden.
- Returntype : Bio::EnsEMBL::Slice
- Exceptions : thrown if the clone is not in the assembly
+ Arg [1] : none
+ Example : @all = @{$slice_adaptor->fetch_all_non_redundant()};
+ Description: Retrieves all non-redundant slices, i.e. those which have
+ the attribute 'nonredundant' set
+ Returntype : listref of Bio::EnsEMBL::Slices
+ Exceptions : none
Caller : general
=cut
-sub fetch_by_clone_accession{
- my ($self,$clone,$size) = @_;
+sub fetch_all_non_redundant {
- if( !defined $clone ) {
- $self->throw("Must have clone to fetch Slice of clone");
- }
- if( !defined $size ) {$size=0;}
-
- my $type = $self->db->assembly_type()
- or $self->throw("No assembly type defined");
-
- my $sth = $self->db->prepare("SELECT c.name,
- a.chr_start,
- a.chr_end,
- chr.name
- FROM assembly a,
- contig c,
- clone cl,
- chromosome chr
- WHERE c.clone_id = cl.clone_id
- AND cl.name = '$clone'
- AND c.contig_id = a.contig_id
- AND a.type = '$type'
- AND chr.chromosome_id = a.chromosome_id
- ORDER BY a.chr_start"
- );
- $sth->execute();
-
- my ($contig,$start,$end,$chr_name);
- my $counter;
- my $first_start;
- while ( my @row=$sth->fetchrow_array){
- $counter++;
- ($contig,$start,$end,$chr_name)=@row;
- if ($counter==1){$first_start=$start;}
- }
+ my $self = shift;
- if( !defined $contig ) {
- $self->throw("Clone is not on the golden path. Cannot build Slice");
- }
-
- $first_start -= $size;
- $end += $size;
+ my $sth = $self->prepare("SELECT s.name, s.length, c.coord_system_id " .
+ "FROM seq_region s, coord_system c, seq_region_attrib sra, attrib_type at " .
+ "WHERE s.coord_system_id=c.coord_system_id " .
+ "AND at.code='nonredundant' " .
+ "AND at.attrib_type_id=sra.attrib_type_id " .
+ "AND sra.seq_region_id=s.seq_region_id");
- if($first_start < 1) {
- $first_start = 1;
- }
+ $sth->execute();
+
+ my ($name, $length, $cs_id);
+ $sth->bind_columns(\$name, \$length, \$cs_id);
+
+ # Slice expects a CoordSystem object
+ my $cs_adaptor = $self->db->get_CoordSystemAdaptor();
+
+
+ my @out;
+ while($sth->fetch()) {
+ my $cs = $cs_adaptor->fetch_by_dbID($cs_id);
+ push @out, Bio::EnsEMBL::Slice->new(-START => 1,
+ -END => $length,
+ -STRAND => 1,
+ -SEQ_REGION_NAME => $name,
+ -COORD_SYSTEM => $cs,
+ -ADAPTOR => $self);
+ }
+
+ return \@out;
+
+}
+
+
+sub deleteObj {
+ my $self = shift;
+
+ $self->SUPER::deleteObj;
- my $slice = $self->fetch_by_chr_start_end($chr_name, $first_start, $end);
- return $slice;
+ $self->{'_id_cache'} = {};
+ $self->{'_name_cache'} = {};
+ $self->{'_exc_cache'} = {};
+}
+
+
+
+=head2 fetch_by_chr_band
+
+ Title : fetch_by_chr_band
+ Usage :
+ Function: create a Slice representing a series of bands
+ Example :
+ Returns :
+ Args : the band name
+
+=cut
+
+sub fetch_by_chr_band {
+ my ($self,$chr,$band) = @_;
+
+ my $chr_slice = $self->fetch_by_region('chromosome', $chr);
+
+ my $seq_region_id = $self->get_seq_region_id($chr_slice);
+
+ my $sth = $self->db->prepare
+ ("select min(k.chr_start), max(k.chr_end) " .
+ "from karyotype as k " .
+ "where k.seq_region_id = ? and k.band like ?");
+
+ $sth->execute( $seq_region_id, "$band%" );
+ my ( $slice_start, $slice_end) = $sth->fetchrow_array;
+
+ if(defined $slice_start) {
+ return $self->fetch_by_region('chromosome',$chr,$slice_start,$slice_end);
+ }
+
+ throw("Band not recognised in database");
}
@@ -363,10 +655,10 @@ sub fetch_by_clone_accession{
Example : $slc = $sa->fetch_by_transcript_stable_id('ENST00000302930',10);
Description: Creates a slice around the region of the specified transcript.
If a context size is given, the slice is extended by that
- number of basepairs on either side of the
- transcript. Throws if the transcript is not golden.
+ number of basepairs on either side of the
+ transcript.
Returntype : Bio::EnsEMBL::Slice
- Exceptions : none
+ Exceptions : Thrown if the transcript is not in the database.
Caller : general
=cut
@@ -374,14 +666,15 @@ sub fetch_by_clone_accession{
sub fetch_by_transcript_stable_id{
my ($self,$transcriptid,$size) = @_;
- # Just get the dbID, then fetch slice by that
+ throw('Transcript argument is required.') if(!$transcriptid);
+
my $ta = $self->db->get_TranscriptAdaptor;
- my $transcript_obj = $ta->fetch_by_stable_id($transcriptid);
- my $dbID = $transcript_obj->dbID;
-
- return $self->fetch_by_transcript_id($dbID, $size);
-}
+ my $transcript = $ta->fetch_by_stable_id($transcriptid);
+ throw("Transcript [$transcriptid] does not exist in DB.") if(!$transcript);
+
+ return $self->fetch_by_Feature($transcript, $size);
+}
@@ -394,12 +687,13 @@ sub fetch_by_transcript_stable_id{
The length of the flanking regions the slice should encompass
on either side of the transcript (0 by default)
Example : $slc = $sa->fetch_by_transcript_id(24, 1000);
- Description: Creates a slice around the region of the specified transcript.
- If a context size is given, the slice is extended by that
- number of basepairs on either side of the
- transcript.
+ Description: Creates a slice around the region of the specified transcript.
+ If a context size is given, the slice is extended by that
+ number of basepairs on either side of the
+ transcript.
Returntype : Bio::EnsEMBL::Slice
- Exceptions : thrown on incorrect args
+ Exceptions : throw on incorrect args
+ throw if transcript is not in database
Caller : general
=cut
@@ -407,39 +701,14 @@ sub fetch_by_transcript_stable_id{
sub fetch_by_transcript_id {
my ($self,$transcriptid,$size) = @_;
- unless( defined $transcriptid ) {
- $self->throw("Must have transcriptid id to fetch Slice of transcript");
- }
+ throw('Transcript id argument is required.') if(!$transcriptid);
- $size = 0 unless(defined $size);
-
- my $ta = $self->db->get_TranscriptAdaptor;
- my $transcript_obj = $ta->fetch_by_dbID($transcriptid);
-
- my %exon_transforms;
-
- my $emptyslice;
- for my $exon ( @{$transcript_obj->get_all_Exons()} ) {
- $emptyslice = Bio::EnsEMBL::Slice->new( '-empty' => 1,
- '-adaptor' => $self,
- '-ASSEMBLY_TYPE' =>
- $self->db->assembly_type);
- my $newExon = $exon->transform( $emptyslice );
- $exon_transforms{ $exon } = $newExon;
- }
-
- $transcript_obj->transform( \%exon_transforms );
-
- my $start = $transcript_obj->start() - $size;
- my $end = $transcript_obj->end() + $size;
-
- if($start < 1) {
- $start = 1;
- }
-
- my $slice = $self->fetch_by_chr_start_end($emptyslice->chr_name,
- $start, $end);
- return $slice;
+ my $transcript_adaptor = $self->db()->get_TranscriptAdaptor();
+ my $transcript = $transcript_adaptor->fetch_by_dbID($transcriptid);
+
+ throw("Transcript [$transcriptid] does not exist in DB.") if(!$transcript);
+
+ return $self->fetch_by_Feature($transcript, $size);
}
@@ -447,7 +716,7 @@ sub fetch_by_transcript_id {
=head2 fetch_by_gene_stable_id
Arg [1] : string $geneid
- The stable id of the gene around which the slice is
+ The stable id of the gene around which the slice is
desired
Arg [2] : (optional) int $size
The length of the flanking regions the slice should encompass
@@ -456,243 +725,620 @@ sub fetch_by_transcript_id {
Description: Creates a slice around the region of the specified gene.
If a context size is given, the slice is extended by that
number of basepairs on either side of the gene.
+ The slice will be created in the genes native coordinate system.
Returntype : Bio::EnsEMBL::Slice
- Exceptions : none
+ Exceptions : throw on incorrect args
+ throw if transcript does not exist
Caller : general
=cut
-sub fetch_by_gene_stable_id{
- my ($self,$geneid,$size) = @_;
+sub fetch_by_gene_stable_id {
+ my ($self,$geneid,$size) = @_;
- if( !defined $geneid ) {
- $self->throw("Must have gene id to fetch Slice of gene");
- }
- my ($chr_name,$start,$end) = $self->_get_chr_start_end_of_gene($geneid);
+ throw('Gene argument is required.') if(!$geneid);
- if( $size =~/([\d+\.]+)%/ ) {
- $size = int($1/100 * ($end-$start+1));
- }
- if( !defined $size ) {$size=0;}
+ my $gene_adaptor = $self->db->get_GeneAdaptor();
+ my $gene = $gene_adaptor->fetch_by_stable_id($geneid);
- if( !defined $start ) {
- my $type = $self->db->assembly_type()
- or $self->throw("No assembly type defined");
- $self->throw("Gene [$geneid] is not on the golden path '$type'. " .
- "Cannot build Slice.");
- }
-
- $start -= $size;
- $end += $size;
-
- if($start < 1) {
- $start = 1;
- }
+ throw("Gene [$geneid] does not exist in DB.") if(!$gene);
- return $self->fetch_by_chr_start_end($chr_name, $start, $end);
+ return $self->fetch_by_Feature($gene, $size);
}
-=head2 fetch_by_chr_name
+=head2 fetch_by_Feature
- Arg [1] : string $chr_name
- Example : $slice = $slice_adaptor->fetch_by_chr_name('20');
- Description: Retrieves a slice on the region of an entire chromosome
+ Arg [1] : Bio::EnsEMBL::Feature $feat
+ The feature to fetch the slice around
+ Arg [2] : int size (optional)
+ The desired number of flanking basepairs around the feature.
+ Example : $slice = $slice_adaptor->fetch_by_Feature($feat, 100);
+ Description: Retrieves a slice around a specific feature. All this really
+ does is return a resized version of the slice that the feature
+ is already on. Note that slices returned from this method
+ are always on the forward strand of the seq_region regardless of
+ the strandedness of the feature passed in.
Returntype : Bio::EnsEMBL::Slice
- Exceptions : thrown if $chr_name arg is not supplied
- Caller : general
+ Exceptions : throw if the feature does not have an attached slice
+ throw if feature argument is not provided
+ Caller : fetch_by_gene_stable_id, fetch_by_transcript_stable_id,
+ fetch_by_gene_id, fetch_by_transcript_id
=cut
-sub fetch_by_chr_name{
- my ($self,$chr_name) = @_;
+sub fetch_by_Feature{
+ my ($self, $feature, $size) = @_;
- unless( $chr_name ) {
- $self->throw("Chromosome name argument required");
- }
+ $size ||= 0;
+
+ if(!ref($feature) || !$feature->isa('Bio::EnsEMBL::Feature')) {
+ throw('Feature argument expected.');
+ }
+
+ my $slice = $feature->slice();
+ if(!$slice || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw('Feature must be attached to a valid slice.');
+ }
+
+ my $fstart = $feature->start();
+ my $fend = $feature->end();
+ if(!defined($fstart) || !defined($fend)) {
+ throw('Feature must have defined start and end.');
+ }
+
+ #convert the feature slice coordinates to seq_region coordinates
+ my $slice_start = $slice->start();
+ my $slice_end = $slice->end();
+ my $slice_strand = $slice->strand();
+ if($slice_start != 1 || $slice_strand != 1) {
+ if($slice_strand == 1) {
+ $fstart = $fstart + $slice_start - 1;
+ $fend = $fend + $slice_start - 1;
+ } else {
+ my $tmp_start = $fstart;
+ $fstart = $slice_end - $fend + 1;
+ $fend = $slice_end - $tmp_start + 1;
+ }
+ }
+
+ #return a new slice covering the region of the feature
+ return Bio::EnsEMBL::Slice->new(-seq_region_name => $slice->seq_region_name,
+ -coord_system => $slice->coord_system,
+ -start => $fstart - $size,
+ -end => $fend + $size,
+ -strand => 1,
+ -adaptor => $self);
+}
+
+
+
+=head2 fetch_by_misc_feature_attribute
+
+ Arg [1] : string $attribute_type
+ The code of the attribute type
+ Arg [2] : (optional) string $attribute_value
+ The value of the attribute to fetch by
+ Arg [3] : (optional) int $size
+ The amount of flanking region around the misc feature desired.
+ Example : $slice = $sa->fetch_by_misc_feature_attribute('superctg',
+ 'NT_030871');
+ $slice = $sa->fetch_by_misc_feature_attribute('synonym',
+ 'AL00012311',
+ $flanking);
+ Description: Fetches a slice around a MiscFeature with a particular
+ attribute type and value. If no value is specified then
+ the feature with the particular attribute is used.
+ If no size is specified then 0 is used.
+ Returntype : Bio::EnsEMBL::Slice
+ Exceptions : Throw if no feature with the specified attribute type and value
+ exists in the database
+ Warning if multiple features with the specified attribute type
+ and value exist in the database.
+ Caller : webcode
+
+=cut
+
+sub fetch_by_misc_feature_attribute {
+ my ($self, $attrib_type_code, $attrib_value, $size) = @_;
- my $chr_start = 1;
-
- #set the end of the slice to the end of the chromosome
- my $ca = $self->db()->get_ChromosomeAdaptor();
- my $chromosome = $ca->fetch_by_chr_name($chr_name);
+ my $mfa = $self->db()->get_MiscFeatureAdaptor();
- $self->throw("Unknown chromosome $chr_name") unless $chromosome;
+ my $feats = $mfa->fetch_all_by_attribute_type_value($attrib_type_code,
+ $attrib_value);
- my $chr_end = $chromosome->length();
+ if(@$feats == 0) {
+ throw("MiscFeature with $attrib_type_code=$attrib_value does " .
+ "not exist in DB.");
+ }
- my $type = $self->db->assembly_type();
+ if(@$feats > 1) {
+ warning("MiscFeature with $attrib_type_code=$attrib_value is " .
+ "ambiguous - using first one found.");
+ }
- my $slice = Bio::EnsEMBL::Slice->new
- (
- -chr_name => $chr_name,
- -chr_start => 1,
- -chr_end => $chr_end,
- -assembly_type => $type,
- -adaptor => $self
- );
+ my ($feat) = @$feats;
- return $slice;
+ return $self->fetch_by_Feature($feat, $size);
}
+=head2 fetch_normalized_slice_projection
-=head2 fetch_by_mapfrag
+ Arg [1] : Bio::EnsEMBL::Slice $slice
+ Example : ( optional )
+ Description: gives back a project style result. The returned slices
+ represent the areas to which there are symlinks for the
+ given slice. start, end show which area on given slice is
+ symlinked
+ Returntype : [[start,end,$slice][]]
+ Exceptions : none
+ Caller : BaseFeatureAdaptor
+
+=cut
+
+
+sub fetch_normalized_slice_projection {
+ my $self = shift;
+ my $slice = shift;
+
+ my $slice_seq_region_id = $self->get_seq_region_id( $slice );
+
+ my $result = $self->{'asm_exc_cache'}->{$slice_seq_region_id};
+
+ if(!defined($result)) {
+ my $sql = "
+ SELECT seq_region_id, seq_region_start, seq_region_end,
+ exc_type, exc_seq_region_id, exc_seq_region_start,
+ exc_seq_region_end
+ FROM assembly_exception
+ WHERE seq_region_id = ?";
+
+ my $sth = $self->prepare( $sql );
+ $sth->execute( $slice_seq_region_id );
+ $result = $sth->fetchall_arrayref();
+ $self->{'asm_exc_cache'}->{$slice_seq_region_id} = $result;
+ }
+
+ my (@haps, @pars);
+
+ foreach my $row (@$result) {
+ my ( $seq_region_id, $seq_region_start, $seq_region_end,
+ $exc_type, $exc_seq_region_id, $exc_seq_region_start,
+ $exc_seq_region_end ) = @$row;
+
+ # need overlapping PAR and all HAPs if any
+ if( $exc_type eq "PAR" ) {
+ if( $seq_region_start <= $slice->end() &&
+ $seq_region_end >= $slice->start() ) {
+ push( @pars, [ $seq_region_start, $seq_region_end, $exc_seq_region_id,
+ $exc_seq_region_start, $exc_seq_region_end ] );
+ }
+ } else {
+ push( @haps, [ $seq_region_start, $seq_region_end, $exc_seq_region_id,
+ $exc_seq_region_start, $exc_seq_region_end ] );
+ }
+ }
+
+ if(!@pars && !@haps) {
+ #just return this slice, there were no haps or pars
+ return [[1,$slice->length, $slice]];
+ }
+
+ my @syms;
+ if( @haps > 1 ) {
+ my @sort_haps = sort { $a->[1] <=> $b->[1] } @haps;
+ throw( "More than one HAP region not supported yet" );
+ } elsif( @haps == 1 ) {
+ my $hap = $haps[0];
+
+ my $seq_reg_slice = $self->fetch_by_seq_region_id($slice_seq_region_id);
+ my $exc_slice = $self->fetch_by_seq_region_id( $hap->[2] );
+
+ #
+ # lengths of haplotype and reference in db may be different
+ # we want to use the maximum possible length for the mapping
+ # between the two systems
+ #
+ my $len1 = $seq_reg_slice->length();
+ my $len2 = $exc_slice->length();
+ my $max_len = ($len1 > $len2) ? $len1 : $len2;
+
+ #the inserted region can differ in length, but mapped sections
+ #need to be same lengths
+ my $diff = $hap->[4] - $hap->[1];
+
+ # we want the region of the haplotype INVERTED
+ push( @syms, [ 1, $hap->[0]-1, $hap->[2], 1, $hap->[3] - 1 ] );
+ push( @syms, [ $hap->[1]+1, $max_len - $diff,
+ $hap->[2], $hap->[4] + 1, $max_len ] );
+ }
+
+ # for now haps and pars should not be both there, but in theory we
+ # could handle it here by cleverly merging the pars into the existing syms,
+ # for now just:
+ push( @syms, @pars );
+
+ my $mapper = Bio::EnsEMBL::Mapper->new( "sym", "org" );
+ for my $sym ( @syms ) {
+ $mapper->add_map_coordinates( $slice_seq_region_id, $sym->[0], $sym->[1],
+ 1, $sym->[2], $sym->[3], $sym->[4] );
+ }
- Title : fetch_by_mapfrag
- Usage : $slice = $slice_adaptor->fetch_by_mapfrag('20');
- Function: Creates a slice of a "mapfrag"
- Returns : Slice object
- Args : chromosome name
+ my @linked = $mapper->map_coordinates( $slice_seq_region_id,
+ $slice->start(), $slice->end(),
+ $slice->strand(), "sym" );
+ # gaps are regions where there is no mapping to another region
+ my $rel_start = 1;
+
+ #if there was only one coord and it is a gap, we know it is just the
+ #same slice with no overlapping symlinks
+ if(@linked == 1 && $linked[0]->isa('Bio::EnsEMBL::Mapper::Gap')) {
+ return [[1,$slice->length, $slice]];
+ }
+
+ my @out;
+ for my $coord ( @linked ) {
+ if( $coord->isa( "Bio::EnsEMBL::Mapper::Gap" )) {
+ my $exc_slice = Bio::EnsEMBL::Slice->new
+ (-START => $coord->start(),
+ -END => $coord->end(),
+ -STRAND => $slice->strand(),
+ -COORD_SYSTEM => $slice->coord_system(),
+ -ADAPTOR => $self,
+ -SEQ_REGION_NAME => $slice->seq_region_name);
+ push( @out, [ $rel_start, $coord->length()+$rel_start-1,
+ $exc_slice ] );
+ } else {
+ my $exc_slice = $self->fetch_by_seq_region_id( $coord->id() );
+ my $exc2_slice = Bio::EnsEMBL::Slice->new
+ (
+ -START => $coord->start(),
+ -END => $coord->end(),
+ -STRAND => $coord->strand(),
+ -SEQ_REGION_NAME => $exc_slice->seq_region_name(),
+ -COORD_SYSTEM => $exc_slice->coord_system(),
+ -ADAPTOR => $self
+ );
+
+ push( @out, [ $rel_start, $coord->length() + $rel_start - 1,
+ $exc2_slice ] );
+ }
+ $rel_start += $coord->length();
+ }
+
+ return \@out;
+}
+
+
+
+
+=head2 store
+
+ Arg [1] : Bio::EnsEMBL::Slice $slice
+ Arg [2] : (optional) $seqref reference to a string
+ The sequence associated with the slice to be stored.
+ Example : $slice = Bio::EnsEMBL::Slice->new(...);
+ $seq_region_id = $slice_adaptor->store($slice, \$sequence);
+ Description: This stores a slice as a sequence region in the database
+ and returns the seq region id. The passed in slice must
+ start at 1, and must have a valid seq_region name and coordinate
+ system. The attached coordinate system must already be stored in
+ the database. The sequence region is assumed to start at 1 and
+ to have a length equalling the length of the slice.
+ If the slice coordinate system is the sequence level coordinate
+ system then the seqref argument must also be passed. If the
+ slice coordinate system is NOT a sequence level coordinate
+ system then the sequence argument cannot be passed.
+ Returntype : int
+ Exceptions : throw if slice has no coord system.
+ throw if slice coord system is not already stored.
+ throw if slice coord system is seqlevel and no sequence is
+ provided.
+ throw if slice coord system is not seqlevel and sequence is
+ provided.
+ throw if slice does not start at 1
+ throw if sequence is provided and the sequence length does not
+ match the slice length.
+ throw if the SQL insert fails (e.g. on duplicate seq region)
+ throw if slice argument is not passed
+ Caller : database loading scripts
+
+=cut
+
+
+
+sub store {
+ my $self = shift;
+ my $slice = shift;
+ my $seqref = shift;
+
+ #
+ # Get all of the sanity checks out of the way before storing anything
+ #
+
+ if(!ref($slice) || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw('Slice argument is required');
+ }
+
+ my $cs = $slice->coord_system();
+ throw("Slice must have attached CoordSystem.") if(!$cs);
+
+ my $db = $self->db();
+ if(!$cs->is_stored($db)) {
+ throw("Slice CoordSystem must already be stored in DB.")
+ }
+
+ if($slice->start != 1 || $slice->strand != 1) {
+ throw("Slice must have start==1 and strand==1.");
+ }
+
+ my $sr_len = $slice->length();
+ my $sr_name = $slice->seq_region_name();
+
+ if(!$sr_name) {
+ throw("Slice must have valid seq region name.");
+ }
+
+ if($cs->is_sequence_level()) {
+ if(!$seqref) {
+ throw("Must provide sequence for sequence level coord system.");
+ }
+ if(ref($seqref) ne 'SCALAR') {
+ throw("Sequence must be a scalar reference.");
+ }
+ my $seq_len = length($$seqref);
+
+ if($seq_len != $sr_len) {
+ throw("Sequence length ($seq_len) must match slice length ($sr_len).");
+ }
+ } else {
+ if($seqref) {
+ throw("Cannot provide sequence for non-sequence level seq regions.");
+ }
+ }
+
+ #store the seq_region
+
+ my $sth = $db->prepare("INSERT INTO seq_region " .
+ "SET name = ?, " .
+ " length = ?, " .
+ " coord_system_id = ?" );
+
+ $sth->execute($sr_name, $sr_len, $cs->dbID());
+
+ my $seq_region_id = $sth->{'mysql_insertid'};
+
+ if(!$seq_region_id) {
+ throw("Database seq_region insertion failed.");
+ }
+
+ if($cs->is_sequence_level()) {
+ #store sequence if it was provided
+ my $seq_adaptor = $db->get_SequenceAdaptor();
+ $seq_adaptor->store($seq_region_id, $$seqref);
+ }
+
+ return $seq_region_id;
+}
+
+
+
+#####################################
+# sub DEPRECATED METHODs
+#####################################
+
+=head2 fetch_by_mapfrag
+
+ Function: DEPRECATED use fetch_by_misc_feature_attribute('synonym',$mapfrag)
=cut
sub fetch_by_mapfrag{
my ($self,$mymapfrag,$flag,$size) = @_;
-
+ deprecate('Use fetch_by_misc_feature_attribute instead');
$flag ||= 'fixed-width'; # alt.. 'context'
- $size ||= $flag eq 'fixed-width' ? 200000 : 0;
- unless( $mymapfrag ) {
- $self->throw("Mapfrag name argument required");
- }
+ $size ||= $flag eq 'fixed-width' ? 100000 : 0;
+ return $self->fetch_by_misc_feature_attribute('synonym',$mymapfrag,$size);
+}
- my( $chr_start,$chr_end);
-
- #set the end of the slice to the end of the chromosome
- my $ca = $self->db()->get_MapFragAdaptor();
- my $mapfrag = $ca->fetch_by_synonym($mymapfrag);
- return undef unless defined $mapfrag;
-
- if( $flag eq 'fixed-width' ) {
- my $halfsize = int( $size/2 );
- $chr_start = $mapfrag->seq_start - $halfsize;
- $chr_end = $mapfrag->seq_start + $size - $halfsize;
- } else {
- $chr_start = $mapfrag->seq_start - $size;
- $chr_end = $mapfrag->seq_end + $size;
- }
- my $type = $self->db->assembly_type();
-
- my $slice = Bio::EnsEMBL::Slice->new
- (
- -chr_name => $mapfrag->seq,
- -chr_start => $chr_start,
- -chr_end => $chr_end,
- -assembly_type => $type,
- -adaptor => $self
- );
-
- return $slice;
+
+
+=head2 fetch_by_chr_start_end
+
+ Description: DEPRECATED use fetch_by_region instead
+
+=cut
+
+sub fetch_by_chr_start_end {
+ my ($self,$chr,$start,$end) = @_;
+ deprecate('Use fetch_by_region() instead');
+
+ #assume that by chromosome the user actually meant top-level coord
+ #system since this is the old behaviour of this deprecated method
+ my $csa = $self->db->get_CoordSystemAdaptor();
+ my $cs = $csa->fetch_top_level();
+
+ return $self->fetch_by_region($cs->name,$chr,$start,$end,1,$cs->version);
}
+=head2 fetch_by_contig_name
+ Description: Deprecated. Use fetch_by_region(), Slice::project(),
+ Slice::expand() instead
-=head2 _get_chr_start_end_of_contig
+=cut
- Title : _get_chr_start_end_of_contig
- Usage :
- Function: returns the chromosome name, absolute start and absolute end of the
- specified contig
- Returns : returns chr,start,end
- Args : contig id
+sub fetch_by_contig_name {
+ my ($self, $name, $size) = @_;
+
+ deprecate('Use fetch_by_region(), Slice::project() and Slice::expand().');
+
+ #previously wanted chromosomal slice on a given contig. Assume this means
+ #a top-level slice on a given seq_region in the seq_level coord system
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $top_level = $csa->fetch_top_level();
+ my $seq_level = $csa->fetch_sequence_level();
+
+ my $seq_lvl_slice = $self->fetch_by_region($seq_level->name(), $name);
+
+ my @projection = @{$seq_lvl_slice->project($top_level->name(),
+ $top_level->version())};
+ if(@projection == 0) {
+ warning("contig $name is not used in ".$top_level->name().' assembly.');
+ return undef;
+ }
+
+ if(@projection > 1) {
+ warning("$name is mapped to multiple locations in " . $top_level->name());
+ }
+
+ return $projection[0]->[2]->expand($size, $size);
+}
+
+
+=head2 fetch_by_clone_accession
+
+ Description: DEPRECATED. Use fetch_by_region, Slice::project, Slice::expand
+ instead.
=cut
-sub _get_chr_start_end_of_contig {
- my ($self,$contigid) = @_;
+sub fetch_by_clone_accession{
+ my ($self,$name,$size) = @_;
- if( !defined $contigid ) {
- $self->throw("Must have contig id to fetch Slice of contig");
- }
-
- my $type = $self->db->assembly_type()
- or $self->throw("No assembly type defined");
-
- my $sth = $self->db->prepare("SELECT c.name,
- a.chr_start,
- a.chr_end,
- chr.name
- FROM assembly a, contig c, chromosome chr
- WHERE c.name = '$contigid'
- AND c.contig_id = a.contig_id
- AND a.type = '$type'
- AND chr.chromosome_id = a.chromosome_id"
- );
- $sth->execute();
- my ($contig,$start,$end,$chr_name) = $sth->fetchrow_array;
-
- if( !defined $contig ) {
- $self->throw("Contig $contigid is not on the golden path of type $type");
- }
+ deprecate('Use fetch_by_region(), Slice::project() and Slice::expand().');
+
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $top_level = $csa->fetch_top_level();
+ my $clone_cs = $csa->fetch_by_name('clone');
+
+ if(!$clone_cs) {
+ warning('Clone coordinate system does not exist for this species');
+ return undef;
+ }
+
+ #this unfortunately needs a version on the end to work
+ if(! ($name =~ /\./)) {
+ my $sth = $self->prepare("SELECT sr.name " .
+ "FROM seq_region sr, coord_system cs " .
+ "WHERE cs.name = 'clone' " .
+ "AND cs.coord_system_id = sr.coord_system_id ".
+ "AND sr.name LIKE '$name.%'");
+ $sth->execute();
+ if(!$sth->rows()) {
+ $sth->finish();
+ throw("Clone $name not found in database");
+ }
+
+ ($name) = $sth->fetchrow_array();
+
+ $sth->finish();
+ }
- return ($chr_name,$start,$end);
+ my $clone = $self->fetch_by_region($clone_cs->name(), $name);
+ my @projection = @{$clone->project($top_level->name(),
+ $top_level->version())};
+ if(@projection == 0) {
+ warning("clone $name is not used in " . $top_level->name() . ' assembly.');
+ return undef;
+ }
+
+ if(@projection > 1) {
+ warning("$name is mapped to multiple locations in " . $top_level->name());
+ }
+
+ return $projection[0]->[2]->expand($size, $size);
}
-=head2 _get_chr_start_end_of_gene
- Title : get_Gene_chr_bp
- Usage :
- Function:
- Returns :
- Args :
+=head2 fetch_by_supercontig_name
+ Description: DEPRECATED. Use fetch_by_region(), Slice::project() and
+ Slice::expand() instead
=cut
+sub fetch_by_supercontig_name {
+ my ($self,$name, $size) = @_;
+
+ deprecate('Use fetch_by_region(), Slice::project() and Slice::expand().');
-sub _get_chr_start_end_of_gene {
- my ($self,$geneid) = @_;
-
- my $type = $self->db->assembly_type()
- or $self->throw("No assembly type defined");
-
- my $sth = $self->db->prepare("SELECT
- if(a.contig_ori=1,(e.contig_start-a.contig_start+a.chr_start),
- (a.chr_start+a.contig_end-e.contig_end)),
- if(a.contig_ori=1,(e.contig_end-a.contig_start+a.chr_start),
- (a.chr_start+a.contig_end-e.contig_start)),
- chr.name
-
- FROM exon e,
- transcript tr,
- exon_transcript et,
- assembly a,
- gene_stable_id gsi,
- chromosome chr
- WHERE e.exon_id=et.exon_id
- AND et.transcript_id =tr.transcript_id
- AND a.contig_id=e.contig_id
- AND a.type = '$type'
- AND tr.gene_id = gsi.gene_id
- AND gsi.stable_id = '$geneid'
- AND a.chromosome_id = chr.chromosome_id"
- );
- $sth->execute();
-
- my ($start,$end,$chr);
- my @start;
- while ( my @row=$sth->fetchrow_array){
- ($start,$end,$chr)=@row;
- push @start,$start;
- push @start,$end;
- }
-
- my @start_sorted=sort { $a <=> $b } @start;
-
- $start=shift @start_sorted;
- $end=pop @start_sorted;
-
- return ($chr,$start,$end);
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $top_level = $csa->fetch_top_level();
+ my $sc_level = $csa->fetch_by_name('supercontig');
+
+ if(!$sc_level) {
+ warning('No supercontig coordinate system exists for this species.');
+ return undef;
+ }
+
+ my $sc_slice = $self->fetch_by_region($sc_level->name(),$name);
+
+ my @projection = @{$sc_slice->project($top_level->name(),
+ $top_level->version())};
+ if(@projection == 0) {
+ warning("Supercontig $name is not used in " . $top_level->name() .
+ ' assembly.');
+ return undef;
+ }
+
+ if(@projection > 1) {
+ warning("$name is mapped to multiple locations in " . $top_level->name());
+ }
+
+ return $projection[0]->[2]->expand($size, $size);
}
-1;
+=head2 list_overlapping_supercontigs
+ Description: DEPRECATED use Slice::project instead
+=cut
+sub list_overlapping_supercontigs {
+ my ($self,$slice) = @_;
+
+ deprecate('Use Slice::project() instead.');
+
+ my $csa = $self->db()->get_CoordSystemAdaptor();
+ my $top_level = $csa->fetch_top_level();
+ my $sc_level = $csa->fetch_by_name('supercontig');
+
+ if(!$sc_level) {
+ warning('No supercontig coordinate system exists for this species.');
+ return undef;
+ }
+
+ my @out;
+ foreach my $seg (@{$slice->project($sc_level->name(), $sc_level->version)}){
+ push @out, $seg->[2]->seq_region_name();
+ }
+
+ return \@out;
+}
+
+
+
+=head2 fetch_by_chr_name
+
+ Description: DEPRECATED. Use fetch by region instead
+
+=cut
+
+sub fetch_by_chr_name{
+ my ($self,$chr_name) = @_;
+ deprecate('Use fetch_by_region() instead.');
+
+ return $self->fetch_by_region('toplevel',$chr_name);
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/SupportingFeatureAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/SupportingFeatureAdaptor.pm
index 17612dca81..81e43185ee 100644
--- a/modules/Bio/EnsEMBL/DBSQL/SupportingFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/SupportingFeatureAdaptor.pm
@@ -54,12 +54,6 @@ sub fetch_all_by_Exon {
my $out = [];
- #throw if this is a sticky exon
- if($exon->isa('Bio::EnsEMBL::StickyExon')) {
- $self->throw('Expected Exon but got StickyExon. ' .
- 'Call get_all_component_Exons first');
- }
-
unless($exon->dbID) {
$self->warn("exon has no dbID can't fetch evidence from db " .
"no relationship exists\n");
@@ -86,14 +80,8 @@ sub fetch_all_by_Exon {
$self->throw("Unknown feature type [$type]\n");
}
- if($exon->contig()->isa("Bio::EnsEMBL::Slice")) {
- #tranform to slice coords
- $feature->transform($exon->contig());
- } else {
- #we might have to convert the features coordinate system
- next unless($feature->contig->dbID == $exon->contig->dbID);
- }
- push @$out, $feature;
+ my $new_feature = $feature->transfer($exon->slice());
+ push @$out, $new_feature if( $new_feature );
}
return $out;
}
diff --git a/modules/Bio/EnsEMBL/DBSQL/TranscriptAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/TranscriptAdaptor.pm
index a1cbe19332..00c7e994e5 100644
--- a/modules/Bio/EnsEMBL/DBSQL/TranscriptAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/TranscriptAdaptor.pm
@@ -11,19 +11,39 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::TranscriptAdaptor - MySQL Database queries to generate and store transcripts/translations.
+Bio::EnsEMBL::DBSQL::TranscriptAdaptor - An adaptor which performs database
+interaction relating to the storage and retrieval of Transcripts
+
+=head1 DESCRIPTION
+
+This adaptor provides a means to retrieve and store information related to
+Transcripts. Primarily this involves the retrieval or storage of
+Bio::EnsEMBL::Transcript objects from a database.
+See Bio::EnsEMBL::Transcript for details of the Transcript class.
=head1 SYNOPSIS
-Transcripts and Translations are stored and fetched in this
-object. Translations never go alone any more. The database only
-accepts them (at the moment) in a transcript.
+ $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+ $slice_adaptor = $db->get_SliceAdaptor();
+
+ $transcript_adaptor = $db->get_TranscriptAdaptor();
+
+ $transcript = $transcript_adaptor->fetch_by_dbID(1234);
+
+ $transcript = $transcript_adaptor->fetch_by_stable_id('ENST00000201961');
+
+ $slice = $slice_adaptor->fetch_by_region('chromosome', '3', 1, 1000000);
+ @transcripts = @{$transcript_adaptor->fetch_all_by_Slice($slice)};
+
+ ($transcript) = @{$transcript_adaptor->fetch_all_by_external_name('BRCA2')};
+
=head1 CONTACT
- Arne Stabenau: stabenau@ebi.ac.uk
- Elia Stupka : elia@ebi.ac.uk
- Ewan Birney :
+ Post questions/comments to the EnsEMBL development list:
+ ensembl-dev@ebi.ac.uk
+
+=head1 METHODS
=cut
@@ -32,65 +52,66 @@ package Bio::EnsEMBL::DBSQL::TranscriptAdaptor;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
-use Bio::EnsEMBL::DBSQL::DBAdaptor;
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
use Bio::EnsEMBL::Gene;
use Bio::EnsEMBL::Exon;
use Bio::EnsEMBL::Transcript;
use Bio::EnsEMBL::Translation;
-@ISA = qw( Bio::EnsEMBL::DBSQL::BaseAdaptor );
+use Bio::EnsEMBL::Utils::Exception qw( deprecate throw warning );
+@ISA = qw( Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor );
-=head2 fetch_by_dbID
- Arg [1] : int $transid
- The unique database id for the transcript to be retrieved.
- Example : $transcript = $transcript_adaptor->fetch_by_dbID(1234);
- Description: Retreives a transcript from the database via its dbID
- Returntype : Bio::EnsEMBL::Transcript
- Exceptions : none
- Caller : general
-=cut
-
-sub fetch_by_dbID {
- my ($self, $transid) = @_;
-
- my $seen = 0;
- my $trans = Bio::EnsEMBL::Transcript->new();
- my $exonAdaptor = $self->db->get_ExonAdaptor();
-
- my $sth = $self->prepare("SELECT exon_id
- FROM exon_transcript
- WHERE transcript_id = ?
- ORDER BY rank");
- $sth->execute($transid);
-
- while( my $rowhash = $sth->fetchrow_hashref) {
- my $exon = $exonAdaptor->fetch_by_dbID($rowhash->{'exon_id'});
- $trans->add_Exon($exon);
- $seen = 1;
- }
+# _tables
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns the names, aliases of the tables to use for queries
+# Returntype : list of listrefs of strings
+# Exceptions : none
+# Caller : internal
- if ($seen == 0 ) {
- $self->throw("transcript $transid is not present in db");
- }
- $trans->dbID($transid);
- $trans->adaptor( $self );
- my $ts = $self->prepare("SELECT translation_id translation_id
- FROM transcript
- WHERE transcript_id = ?");
- $ts->execute($transid);
- my ($val) = $ts->fetchrow_array();
- $trans->_translation_id($val);
+sub _tables {
+ my $self = shift;
+
+ return ([ 'transcript', 't' ], [ 'transcript_stable_id', 'tsi' ],
+ [ 'xref', 'x' ], [ 'external_db' , 'exdb' ] );
+}
+
+
+#_columns
+#
+# Arg [1] : none
+# Example : none
+# Description: PROTECTED implementation of superclass abstract method
+# returns a list of columns to use for queries
+# Returntype : list of strings
+# Exceptions : none
+# Caller : internal
+
+sub _columns {
+ my $self = shift;
- return $trans;
+ return qw( t.transcript_id t.seq_region_id t.seq_region_start t.seq_region_end
+ t.seq_region_strand t.gene_id
+ t.display_xref_id tsi.stable_id tsi.version
+ x.display_label exdb.db_name exdb.status );
}
+sub _left_join {
+ return ( [ 'transcript_stable_id', "tsi.transcript_id = t.transcript_id" ],
+ [ 'xref', "x.xref_id = t.display_xref_id" ],
+ [ 'external_db', "exdb.external_db_id = x.external_db_id" ] );
+}
+
+
+
=head2 fetch_by_stable_id
Arg [1] : string $stable_id
@@ -103,20 +124,22 @@ sub fetch_by_dbID {
=cut
-sub fetch_by_stable_id {
- my ($self, $stable_id) = @_;
- my $sth = $self->prepare( "SELECT transcript_id
- FROM transcript_stable_id
- WHERE stable_id = ?" );
- $sth->execute( $stable_id );
+sub fetch_by_stable_id{
+ my ($self,$id) = @_;
+
+ # because of the way this query is constructed (with a left join to the
+ # transcript_stable_id table), it is faster to do 2 queries, getting the
+ # transcript_id in the first query
+ my $sth = $self->prepare("SELECT transcript_id from transcript_stable_id ".
+ "WHERE stable_id = ?");
+ $sth->execute($id);
+
+ my ($dbID) = $sth->fetchrow_array();
+
+ return undef if(!$dbID);
+
+ return $self->fetch_by_dbID($dbID);
- if( my $arr = $sth->fetchrow_arrayref ) {
- my $transcript = $self->fetch_by_dbID( $arr->[0] );
- return $transcript;
- } else {
- $ self->warn( "No Transcript with this stable id found in the database." );
- return undef;
- }
}
@@ -136,64 +159,120 @@ sub fetch_by_stable_id {
=cut
sub fetch_by_translation_stable_id {
- my ($self, $transl_stable_id) = @_;
+ my ($self, $transl_stable_id ) = @_;
- my $sth = $self->prepare( "SELECT t.transcript_id
- FROM translation_stable_id tsi, transcript t
- WHERE tsi.stable_id = ?
- AND t.translation_id = tsi.translation_id");
+ my $sth = $self->prepare( "SELECT t.transcript_id " .
+ "FROM translation_stable_id tsi, translation t ".
+ "WHERE tsi.stable_id = ? " .
+ "AND t.translation_id = tsi.translation_id");
- $sth->execute($transl_stable_id);
+ $sth->execute("$transl_stable_id");
my ($id) = $sth->fetchrow_array;
if ($id){
- return $self->fetch_by_dbID($id);
+ return $self->fetch_by_dbID($id);
} else {
- return undef;
+ return undef;
}
-}
+}
+
+
=head2 fetch_by_translation_id
+ Arg [1] : int $id
+ The internal identifier of the translation whose transcript
+ is to be retrieved.
+ Example : $tr = $tr_adaptor->fetch_by_translation_id($transl->dbID());
+ Description: Given the internal identifier of a translation this method
+ retrieves the transcript associated with that translation.
+ If the transcript cannot be found undef is returned instead.
+ Returntype : Bio::EnsEMBL::Transcript or undef
+ Exceptions : none
+ Caller : general
+
=cut
sub fetch_by_translation_id {
- my ($self, $transl_id) = @_;
- my $sth = $self->prepare(
- "SELECT transcript_id
- FROM transcript
- WHERE translation_id = ?");
- $sth->execute($transl_id);
- my ($id) = $sth->fetchrow_array;
- return $id? $self->fetch_by_dbID($id) : undef;
+ my $self = shift;
+ my $id = shift;
+
+ throw("id argument is required.") if(!$id);
+
+ my $sth = $self->prepare( "SELECT t.transcript_id " .
+ "FROM translation t ".
+ "WHERE t.translation_id = ?");
+
+ $sth->execute($id);
+
+ my ($dbID) = $sth->fetchrow_array;
+ if ($dbID){
+ return $self->fetch_by_dbID($id);
+ } else {
+ return undef;
+ }
}
-=head2 fetch_all_by_DBEntry
- Arg [1] : in $external_id
- the external identifier for the transcript to be obtained
- Example : @trans = @{$trans_adaptor->fetch_all_by_DBEntry($ext_id)}
- Description: retrieves a list of transcripts with an external database
- idenitifier $external_id
- Returntype : listref of Bio::EnsEMBL::DBSQL::Transcript in contig coordinates
+
+=head2 fetch_all_by_Gene
+
+ Arg [1] : Bio::EnsEMBL::Gene $gene
+ Example : none
+ Description: retrieves Transcript objects for given gene. Puts Genes slice
+ in each Transcript.
+ Returntype : listref Bio::EnsEMBL::Transcript
Exceptions : none
- Caller : ?
+ Caller : Gene->get_all_Transcripts()
=cut
-sub fetch_all_by_DBEntry {
+
+sub fetch_all_by_Gene {
+ my $self = shift;
+ my $gene = shift;
+
+ # should be on the same slice as gene
+ my $constraint = "t.gene_id = ".$gene->dbID();
+
+ my $slice = $gene->slice();
+ my $keep_all = 1; #keep transcripts that fall off end of slice
+ return $self->generic_fetch( $constraint, undef, $slice, $keep_all );
+}
+
+
+
+
+=head2 fetch_all_by_external_name
+
+ Arg [1] : string $external_id
+ An external identifier of the transcript to be obtained
+ Example : @trans = @{$tr_adaptor->fetch_all_by_external_name('ARSE')};
+ Description: Retrieves all transcripts which are associated with an
+ external identifier such as a GO term, HUGO id, Swissprot
+ identifer, etc. Usually there will only be a single transcript
+ returned in the listref, but not always. Transcripts are
+ returned in their native coordinate system. That is, the
+ coordinate system in which they are stored in the database. If
+ they are required in another coordinate system the
+ Transcript::transfer or Transcript::transform method can be
+ used to convert them. If no transcripts with the external
+ identifier are found, a reference to an empty list is returned.
+ Returntype : reference to a list of transcripts
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub fetch_all_by_external_name {
my $self = shift;
my $external_id = shift;
+
my @trans = ();
my $entryAdaptor = $self->db->get_DBEntryAdaptor();
- my @ids = $entryAdaptor->transcriptids_by_extids($external_id);
- foreach my $trans_id ( @ids ) {
- my $trans = $self->fetch_by_dbID( $trans_id );
- if( $trans ) {
- push( @trans, $trans );
- }
- }
- return \@trans;
+ my @ids = $entryAdaptor->list_transcript_ids_by_extids($external_id);
+
+ return $self->fetch_all_by_dbID_list(\@ids);
}
@@ -210,24 +289,24 @@ sub fetch_all_by_DBEntry {
=cut
sub fetch_all_by_exon_stable_id {
- my ($self, $stable_id) = @_;
+ my ($self, $stable_id ) = @_;
my @trans ;
my $sth = $self->prepare( qq( SELECT et.transcript_id
- FROM exon_transcript as et,
- exon_stable_id as esi
- WHERE esi.exon_id = et.exon_id and
- esi.stable_id = "$stable_id" ));
- $sth->execute( );
-
- while( my $id = $sth->fetchrow_array ) {
- my $transcript = $self->fetch_by_dbID( $id );
- push(@trans, $transcript) if $transcript;
+ FROM exon_transcript as et,
+ exon_stable_id as esi
+ WHERE esi.exon_id = et.exon_id and
+ esi.stable_id = ? ));
+ $sth->execute("$stable_id");
+
+ while( my $id = $sth->fetchrow_array ) {
+ my $transcript = $self->fetch_by_dbID( $id );
+ push(@trans, $transcript) if $transcript;
+ }
- }
if (!@trans) {
- $self->warn( "No Transcript with this exon stable id found in the database." );
return undef;
}
+
return \@trans;
}
@@ -252,70 +331,129 @@ sub store {
my ($self,$transcript,$gene_dbID) = @_;
if( ! ref $transcript || !$transcript->isa('Bio::EnsEMBL::Transcript') ) {
- $self->throw("$transcript is not a EnsEMBL transcript - not dumping!");
+ throw("$transcript is not a EnsEMBL transcript - not storing");
}
- # store translation
- # then store the transcript
- # then store the exon_transcript table
+ my $db = $self->db();
- my $translation = $transcript->translation();
- my ( $exon_count, $exons );
- $exons = $transcript->get_all_Exons();
- $exon_count = scalar( @{$exons} );
+ if($transcript->is_stored($db)) {
+ return $transcript->dbID();
+ }
+
+ #force lazy-loading of exons and ensure coords are correct
+ $transcript->recalculate_coordinates();
- my $exonAdaptor = $self->db->get_ExonAdaptor();
+ #
+ # Store exons - this needs to be done before the possible transfer
+ # of the transcript to another slice (in _prestore()).Transfering results
+ # in copies being made of the exons and we need to preserve the object
+ # identity of the exons so that they are not stored twice by different
+ # transcripts.
+ #
+ my $exons = $transcript->get_all_Exons();
+ my $exonAdaptor = $db->get_ExonAdaptor();
foreach my $exon ( @{$exons} ) {
$exonAdaptor->store( $exon );
}
- if( defined $translation ) {
- $self->db->get_TranslationAdaptor()->store( $translation );
- }
-
+ my $original = $transcript;
+ my $seq_region_id;
+ ($transcript, $seq_region_id) = $self->_pre_store($transcript);
# first store the transcript w/o a display xref
- # the display xref needs to be set after xrefs are stored which needs to
+ # the display xref needs to be set after xrefs are stored which needs to
# happen after transcript is stored...
my $xref_id = 0;
- # ok - now load this line in
- my $tst = $self->prepare("
- insert into transcript ( gene_id, translation_id,
- exon_count, display_xref_id )
- values ( ?, ?, ?, 0)
- ");
+ #
+ #store transcript
+ #
+ my $tst = $self->prepare(
+ "insert into transcript ( gene_id, seq_region_id, seq_region_start, " .
+ "seq_region_end, seq_region_strand ) " .
+ "values ( ?, ?, ?, ?, ? )");
- if( defined $translation ) {
- $tst->execute( $gene_dbID, $translation->dbID(), $exon_count );
- } else {
- $tst->execute( $gene_dbID, 0, $exon_count );
- }
+ $tst->execute( $gene_dbID, $seq_region_id, $transcript->start(),
+ $transcript->end(), $transcript->strand() );
+ $tst->finish();
my $transc_dbID = $tst->{'mysql_insertid'};
- #store the xrefs/object xref mapping
- my $dbEntryAdaptor = $self->db->get_DBEntryAdaptor();
+ #
+ # store translation
+ #
+ my $translation = $transcript->translation();
+ if( defined $translation ) {
+ #make sure that the start and end exon are set correctly
+ my $start_exon = $translation->start_Exon();
+ my $end_exon = $translation->end_Exon();
+
+ if(!$start_exon) {
+ throw("Translation does not define a start exon.");
+ }
+
+ if(!$end_exon) {
+ throw("Translation does not defined an end exon.");
+ }
+
+ #If the dbID is not set, this means the exon must have been a different
+ #object in memory than the the exons of the transcript. Try to find the
+ #matching exon in all of the exons we just stored
+ if(!$start_exon->dbID()) {
+ my $key = $start_exon->hashkey();
+ ($start_exon) = grep {$_->hashkey() eq $key} @$exons;
+
+ if($start_exon) {
+ $translation->start_Exon($start_exon);
+ } else {
+ throw("Translation's start_Exon does not appear to be one of the " .
+ "exons in its associated Transcript");
+ }
+ }
+
+ if(!$end_exon->dbID()) {
+ my $key = $end_exon->hashkey();
+ ($end_exon) = grep {$_->hashkey() eq $key} @$exons;
+
+ if($start_exon) {
+ $translation->end_Exon($end_exon);
+ } else {
+ throw("Translation's end_Exon does not appear to be one of the " .
+ "exons in its associated Transcript.");
+ }
+ }
+
+ $db->get_TranslationAdaptor()->store( $translation, $transc_dbID );
+ }
+
+ #
+ # store the xrefs/object xref mapping
+ #
+ my $dbEntryAdaptor = $db->get_DBEntryAdaptor();
foreach my $dbe ( @{$transcript->get_all_DBEntries} ) {
$dbEntryAdaptor->store( $dbe, $transc_dbID, "Transcript" );
}
#
- # Update transcript to point to display xref if it is set
+ # Update transcript to point to display xref if it is set
#
if(my $dxref = $transcript->display_xref) {
if(my $dxref_id = $dbEntryAdaptor->exists($dxref)) {
my $sth = $self->prepare( "update transcript set display_xref_id = ?".
" where transcript_id = ?");
$sth->execute($dxref_id, $transc_dbID);
+ $sth->finish();
$dxref->dbID($dxref_id);
$dxref->adaptor($dbEntryAdaptor);
}
}
- my $etst =
+ #
+ # Link transcript to exons in exon_transcript table
+ #
+ my $etst =
$self->prepare("insert into exon_transcript (exon_id,transcript_id,rank)"
." values (?,?,?)");
my $rank = 1;
@@ -324,9 +462,14 @@ sub store {
$rank++;
}
+ $etst->finish();
+
+ #
+ # Store stable_id
+ #
if (defined($transcript->stable_id)) {
if (!defined($transcript->version)) {
- $self->throw("Trying to store incomplete stable id information for " .
+ throw("Trying to store incomplete stable id information for " .
"transcript");
}
@@ -335,44 +478,21 @@ sub store {
" VALUES(?, ?, ?)";
my $sth = $self->prepare($statement);
$sth->execute($transc_dbID, $transcript->stable_id, $transcript->version);
+ $sth->finish();
}
- $transcript->dbID( $transc_dbID );
- $transcript->adaptor( $self );
+ #update the original transcript object - not the transfered copy that
+ #we might have created
+ $original->dbID( $transc_dbID );
+ $original->adaptor( $self );
+
return $transc_dbID;
}
-=head2 get_stable_entry_info
-
- Title : get_stable_entry_info
- Usage : $transcriptAdptor->get_stable_entry_info($transcript)
- Function: gets stable info for gene and places it into the hash
- Returns :
- Args :
-=cut
-
-sub get_stable_entry_info {
- my ($self,$transcript) = @_;
-
- unless( defined $transcript && ref $transcript &&
- $transcript->isa('Bio::EnsEMBL::Transcript') ) {
- $self->throw("Needs a Transcript object, not a $transcript");
- }
-
- my $sth = $self->prepare("SELECT stable_id, version
- FROM transcript_stable_id
- WHERE transcript_id = ?");
- $sth->execute($transcript->dbID());
- my @array = $sth->fetchrow_array();
- $transcript->{'_stable_id'} = $array[0];
- $transcript->{'_version'} = $array[1];
-
- return 1;
-}
=head2 get_Interpro_by_transid
@@ -390,22 +510,21 @@ sub get_stable_entry_info {
sub get_Interpro_by_transid {
my ($self,$transid) = @_;
- my $sql="
- SELECT i.interpro_ac,
- x.description
- FROM transcript t,
- protein_feature pf,
- interpro i,
- xref x,
- transcript_stable_id tsi
- WHERE tsi.stable_id = '$transid'
- AND t.transcript_id = tsi.transcript_id
- AND t.translation_id = pf.translation_id
- AND i.id = pf.hit_id
- AND i.interpro_ac = x.dbprimary_acc";
-
- my $sth = $self->prepare($sql);
- $sth->execute;
+
+ my $sth = $self->prepare
+ ("SELECT STRAIGHT_JOIN i.interpro_ac, x.description " .
+ "FROM transcript_stable_id tsi, ".
+ "translation tl, ".
+ "protein_feature pf, ".
+ "interpro i, " .
+ "xref x " .
+ "WHERE tsi.stable_id = ? " .
+ "AND tl.transcript_id = tsi.transcript_id " .
+ "AND tl.translation_id = pf.translation_id " .
+ "AND i.id = pf.hit_id " .
+ "AND i.interpro_ac = x.dbprimary_acc");
+
+ $sth->execute($transid);
my @out;
my %h;
@@ -416,7 +535,6 @@ sub get_Interpro_by_transid {
push(@out,$string);
}
-
return \@out;
}
@@ -458,69 +576,18 @@ sub remove {
if($count == 0){
$exonAdaptor->remove( $exon );
} else{
- $self->warn("exon " . $exon->dbID . " is not exclusive to transcript " .
+ warning("exon " . $exon->dbID . " is not exclusive to transcript " .
$transcript->dbID . "\n");
}
}
-
- $transcript->{'dbID'} = undef;
-}
-
-
-
-
-
-=head2 get_display_xref
-
- Arg [1] : int $dbID
- the database identifier of the transcript for which the name
- of external db from which its external name is derived.
- Example : $external_dbname = $transcript_adaptor->get_display_xref_id(42);
- Description: Retrieves the display_xref_id for a transcript.
- Returntype : int
- Exceptions : thrown if $dbId arg is not defined
- Caller : general
-
-=cut
-
-sub get_display_xref {
- my ($self, $transcript) = @_;
-
- if( !defined $transcript ) {
- $self->throw("Must call with a Transcript object");
- }
-
- my $sth = $self->prepare("SELECT e.db_name,
- x.display_label,
- x.xref_id
- FROM transcript t,
- xref x,
- external_db e
- WHERE t.transcript_id = ?
- AND t.display_xref_id = x.xref_id
- AND x.external_db_id = e.external_db_id
- ");
- $sth->execute( $transcript->dbID );
-
-
- my ($db_name, $display_label, $xref_id ) = $sth->fetchrow_array();
- if( !defined $xref_id ) {
- return undef;
- }
-
- my $db_entry = Bio::EnsEMBL::DBEntry->new
- (
- -dbid => $xref_id,
- -adaptor => $self->db->get_DBEntryAdaptor(),
- -dbname => $db_name,
- -display_id => $display_label
- );
- return $db_entry;
+ $transcript->dbID(undef);
+ $transcript->adaptor(undef);
}
+
=head2 update
Arg [1] : Bio::EnsEMBL::Transcript
@@ -540,15 +607,15 @@ sub update {
my ($self,$transcript) = @_;
my $update = 0;
- if( !defined $transcript || !ref $transcript || !$transcript->isa('Bio::EnsEMBL::Transcript') ) {
- $self->throw("Must update a transcript object, not a $transcript");
+ if( !defined $transcript || !ref $transcript ||
+ !$transcript->isa('Bio::EnsEMBL::Transcript') ) {
+ throw("Must update a transcript object, not a $transcript");
}
- my $sth = $self->prepare("UPDATE transcript
- SET display_xref_id = ?,
- exon_count = ?
- WHERE transcript_id = ?
- ");
+ my $update_transcript_sql = "
+ UPDATE transcript
+ SET display_xref_id = ?
+ WHERE transcript_id = ?";
my $display_xref = $transcript->display_xref();
my $display_xref_id;
@@ -556,11 +623,11 @@ sub update {
if( defined $display_xref && $display_xref->dbID() ) {
$display_xref_id = $display_xref->dbID();
} else {
- $display_xref_id = 0;
+ $display_xref_id = undef;
}
- my $exon_count = scalar( @{$transcript->get_all_Exons()} );
- $sth->execute( $display_xref_id, $exon_count, $transcript->dbID() );
+ my $sth = $self->prepare( $update_transcript_sql );
+ $sth->execute( $display_xref_id, $transcript->dbID() );
}
=head2 list_dbIDs
@@ -583,9 +650,10 @@ sub list_dbIDs {
=head2 list_stable_dbIDs
Arg [1] : none
- Example : @stable_transcript_ids = @{$transcript_adaptor->list_stable_dbIDs()};
- Description: Gets an array of stable ids for all transcripts in the current db
- Returntype : list of ints
+ Example : @stable_trans_ids = @{$transcript_adaptor->list_stable_dbIDs()};
+ Description: Gets an array of stable ids for all transcripts in the current
+ database.
+ Returntype : listref of ints
Exceptions : none
Caller : ?
@@ -598,6 +666,277 @@ sub list_stable_ids {
}
+#_objs_from_sth
+
+# Arg [1] : StatementHandle $sth
+# Example : none
+# Description: PROTECTED implementation of abstract superclass method.
+# responsible for the creation of Transcripts
+# Returntype : listref of Bio::EnsEMBL::Transcripts in target coord system
+# Exceptions : none
+# Caller : internal
+
+sub _objs_from_sth {
+ my ($self, $sth, $mapper, $dest_slice, $keep_all) = @_;
+
+ #
+ # This code is ugly because an attempt has been made to remove as many
+ # function calls as possible for speed purposes. Thus many caches and
+ # a fair bit of gymnastics is used.
+ #
+
+ my $sa = $self->db()->get_SliceAdaptor();
+ my $dbEntryAdaptor = $self->db()->get_DBEntryAdaptor();
+
+ my @transcripts;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
+
+ my ( $transcript_id, $seq_region_id, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $gene_id,
+ $display_xref_id, $stable_id, $version,
+ $external_name, $external_db, $external_status );
+
+ $sth->bind_columns( \$transcript_id, \$seq_region_id, \$seq_region_start,
+ \$seq_region_end, \$seq_region_strand, \$gene_id,
+ \$display_xref_id, \$stable_id, \$version,
+ \$external_name, \$external_db, \$external_status );
+
+
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ my $dest_slice_cs;
+ my $asma;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ $dest_slice_cs = $dest_slice->coord_system();
+ $asma = $self->db->get_AssemblyMapperAdaptor();
+ }
+
+ FEATURE: while($sth->fetch()) {
+
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+ my $dest_mapper = $mapper;
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
+ }
+
+ #obtain a mapper if none was defined, but a dest_seq_region was
+ if(!$dest_mapper && $dest_slice &&
+ !$dest_slice_cs->equals($slice->coord_system)) {
+ $dest_mapper = $asma->fetch_by_CoordSystems($dest_slice_cs,
+ $slice->coord_system);
+ $asm_cs = $dest_mapper->assembled_CoordSystem();
+ $cmp_cs = $dest_mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($dest_mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end,$seq_region_strand) =
+ $dest_mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ $seq_region_strand, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ $seq_region_strand *= -1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE if(!$keep_all);
+ }
+ }
+
+ $slice = $dest_slice;
+ }
+
+ my $display_xref;
+
+ if( $display_xref_id ) {
+ $display_xref = bless
+ { 'dbID' => $display_xref_id,
+ 'adaptor' => $dbEntryAdaptor,
+ 'display_id' => $external_name,
+ 'dbname' => $external_db
+ }, "Bio::EnsEMBL::DBEntry";
+ }
+
+
+ #finally, create the new transcript
+ push @transcripts, Bio::EnsEMBL::Transcript->new
+ ( '-start' => $seq_region_start,
+ '-end' => $seq_region_end,
+ '-strand' => $seq_region_strand,
+ '-adaptor' => $self,
+ '-slice' => $slice,
+ '-dbID' => $transcript_id,
+ '-stable_id' => $stable_id,
+ '-version' => $version,
+ '-external_name' => $external_name,
+ '-external_db' => $external_db,
+ '-external_status' => $external_status,
+ '-display_xref' => $display_xref );
+ }
+
+ return \@transcripts;
+}
+
+
+
+=head2 get_display_xref
+
+ Description: DEPRECATED use $transcript->display_xref()
+
+=cut
+
+sub get_display_xref {
+ my ($self, $transcript) = @_;
+
+ deprecate( "display_xref should be retreived from Transcript object directly." );
+
+ if( !defined $transcript ) {
+ throw("Must call with a Transcript object");
+ }
+
+ my $sth = $self->prepare("SELECT e.db_name,
+ x.display_label,
+ x.xref_id
+ FROM transcript t,
+ xref x,
+ external_db e
+ WHERE t.transcript_id = ?
+ AND t.display_xref_id = x.xref_id
+ AND x.external_db_id = e.external_db_id
+ ");
+ $sth->execute( $transcript->dbID );
+
+
+ my ($db_name, $display_label, $xref_id ) = $sth->fetchrow_array();
+ if( !defined $xref_id ) {
+ return undef;
+ }
+
+ my $db_entry = Bio::EnsEMBL::DBEntry->new
+ (
+ -dbid => $xref_id,
+ -adaptor => $self->db->get_DBEntryAdaptor(),
+ -dbname => $db_name,
+ -display_id => $display_label
+ );
+
+ return $db_entry;
+}
+
+
+
+
+=head2 get_stable_entry_info
+
+ Description: DEPRECATED Use $transcript->stable_id()
+
+=cut
+
+sub get_stable_entry_info {
+ my ($self,$transcript) = @_;
+
+ deprecate( "Stable ids should be loaded directly now" );
+
+ unless( defined $transcript && ref $transcript &&
+ $transcript->isa('Bio::EnsEMBL::Transcript') ) {
+ throw("Needs a Transcript object, not a $transcript");
+ }
+
+ my $sth = $self->prepare("SELECT stable_id, version
+ FROM transcript_stable_id
+ WHERE transcript_id = ?");
+ $sth->execute($transcript->dbID());
+
+ my @array = $sth->fetchrow_array();
+ $transcript->{'_stable_id'} = $array[0];
+ $transcript->{'_version'} = $array[1];
+
+
+ return 1;
+}
+
+
+
+
+=head2 fetch_all_by_DBEntry
+
+ Description: DEPRECATED this method has been renamed
+ fetch_all_by_external_name
+
+=cut
+
+sub fetch_all_by_DBEntry {
+ my $self = shift;
+ deprecate('Use fetch_all_by_external_name instead.');
+ return $self->fetch_all_by_external_name(@_);
+}
+
1;
diff --git a/modules/Bio/EnsEMBL/DBSQL/TranslationAdaptor.pm b/modules/Bio/EnsEMBL/DBSQL/TranslationAdaptor.pm
index 068d237627..3dd7d9adb6 100644
--- a/modules/Bio/EnsEMBL/DBSQL/TranslationAdaptor.pm
+++ b/modules/Bio/EnsEMBL/DBSQL/TranslationAdaptor.pm
@@ -9,20 +9,38 @@
=head1 NAME
-Bio::EnsEMBL::DBSQL::TranslationAdaptor - MySQL Database queries to generate
-and store translations.
+Bio::EnsEMBL::DBSQL::TranslationAdaptor - Provides a means to fetch and store
+Translation objects from a database.
+
+=head1 DESCRIPTION
+
+This adaptor provides a means to retrieve and store Bio::EnsEMBL::Translation
+objects from/in a database.
+
+Translation objects only truly make sense in the context of their transcripts
+so the recommended means to retrieve Translations is by retrieving the
+Transcript object first, and then fetching the Translation.
=head1 SYNOPSIS
-Translations are stored and fetched with this
-object.
+ my $db = Bio::EnsEMBL::DBSQL::DBAdaptor->new(...);
+
+ my $transcript_adaptor = $db->get_TranscriptAdaptor();
+ my $translation_adaptor = $db->get_TranslationAdaptor();
+ my $transcript = $transcript_adaptor->fetch_by_dbID(131243);
+ my $translation = $translation_adaptor->fetch_by_Transcript($transcript);
+
+ print "Translation Start Site: " .
+ $translation->start_Exon()->stable_id(), " ", $translation->start();
+ print "Translation Stop: " .
+ $translation->end_Exon()->stable_id(), " ", $translation->end();
+
=head1 CONTACT
ensembl-dev@ebi.ac.uk
-
-=head1 APPENDIX
+=head1 METHODS
=cut
@@ -31,106 +49,128 @@ package Bio::EnsEMBL::DBSQL::TranslationAdaptor;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::DBSQL::BaseAdaptor;
-use Bio::EnsEMBL::DBSQL::DBAdaptor;
-use Bio::EnsEMBL::Gene;
-use Bio::EnsEMBL::Exon;
-use Bio::EnsEMBL::Transcript;
+use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
use Bio::EnsEMBL::Translation;
+use Bio::EnsEMBL::Utils::Exception qw( throw warning deprecate );
+
@ISA = qw( Bio::EnsEMBL::DBSQL::BaseAdaptor );
-=head2 fetch_by_dbID
- Title : fetch_by_dbID
- Usage :
- Function:
- Example :
- Returns :
- Args :
+=head2 fetch_by_Transcript
+
+ Arg [1] : none, string, int, Bio::EnsEMBL::Example $formal_parameter_name
+ Additional description lines
+ list, listref, hashref
+ Example : ( optional )
+ Description: testable description
+ Returntype : none, txt, int, float, Bio::EnsEMBL::Example
+ Exceptions : none
+ Caller : object::methodname or just methodname
=cut
-sub fetch_by_dbID {
- my ($self,$dbID,$transcript) = @_;
+sub fetch_by_Transcript {
+ my ( $self, $transcript ) = @_;
+ if(!ref($transcript) || !$transcript->isa('Bio::EnsEMBL::Transcript')) {
+ throw("Bio::EnsEMBL::Transcript argument is required.");
+ }
- if( !defined $transcript ) {
- $self->throw("Translations make no sense outside of their " .
- "parent Transcript objects. You must retrieve " .
- "with Transcript parent");
- }
+ my $sql = "
+ SELECT tl.translation_id, tl.start_exon_id,
+ tl.end_exon_id, tl.seq_start, tl.seq_end,
+ tlsi.stable_id, tlsi.version
+ FROM translation tl
+ LEFT JOIN translation_stable_id tlsi
+ ON tlsi.translation_id = tl.translation_id
+ WHERE tl.transcript_id = ?";
+
+ my $transcript_id = $transcript->dbID();
+ my $sth = $self->prepare( $sql );
+ $sth->execute( $transcript_id );
+
+ my ( $translation_id, $start_exon_id, $end_exon_id,
+ $seq_start, $seq_end, $stable_id, $version ) =
+ $sth->fetchrow_array();
+
+ if( ! defined $translation_id ) {
+ return undef;
+ }
- my $sth = $self->prepare("SELECT translation_id tlid, seq_start,
- start_exon_id, seq_end, end_exon_id
- FROM translation
- WHERE translation_id = ?");
- $sth->execute($dbID);
- my $rowhash = $sth->fetchrow_hashref;
+ my ($start_exon, $end_exon);
- if( !defined $rowhash ) {
- # assumme this is a translationless transcript deliberately
- return undef;
- }
+ # this will load all the exons whenever we load the translation
+ # but I guess thats ok ....
- my $out = Bio::EnsEMBL::Translation->new();
+ foreach my $exon (@{$transcript->get_all_Exons()}) {
+ if($exon->dbID() == $start_exon_id ) {
+ $start_exon = $exon;
+ }
- $out->start ($rowhash->{'seq_start'});
- $out->end ($rowhash->{'seq_end'});
+ if($exon->dbID() == $end_exon_id ) {
+ $end_exon = $exon;
+ }
+ }
+ unless($start_exon && $end_exon) {
+ throw("Could not find start or end exon in transcript\n");
+ }
- #search through the transcript's exons for the start and end exons
- my ($start_exon, $end_exon);
- foreach my $exon (@{$transcript->get_all_Exons()}) {
- if($exon->dbID() == $rowhash->{'start_exon_id'}) {
- $start_exon = $exon;
- }
+ my $translation = Bio::EnsEMBL::Translation->new
+ (
+ -dbID => $translation_id,
+ -adaptor => $self,
+ -seq_start => $seq_start,
+ -seq_end => $seq_end,
+ -start_exon => $start_exon,
+ -end_exon => $end_exon,
+ -stable_id => $stable_id,
+ -version => $version
+ );
+
+ return $translation;
+}
- if($exon->dbID() == $rowhash->{'end_exon_id'}) {
- $end_exon = $exon;
- }
- }
- unless($start_exon && $end_exon) {
- $self->throw("Could not find start or end exon in transcript\n");
- }
- $out->start_Exon($start_exon);
- $out->end_Exon($end_exon);
- $out->dbID($rowhash->{'tlid'});
- $out->adaptor( $self );
-
- return $out;
-}
-=head2 fetch_all_by_DBEntry
- Arg [1] : in $external_id
- the external identifier for the translation to be obtained
- Example : @trans = @{$trans_adaptor->fetch_all_by_DBEntry($ext_id)}
- Description: retrieves a list of translation with an external database
- idenitifier $external_id
- Returntype : listref of Bio::EnsEMBL::DBSQL::Translation in contig coordinates
+=head2 fetch_all_by_external_name
+
+ Arg [1] : string $external_id
+ The external identifier for the tranlsation(s) to be obtained.
+ Example : @tls = @{$trl_adaptor->fetch_all_by_external_name('BRCA2')};
+ Description: Retrieves a list of translations fetched via an external
+ identifier. Note that this may not be a particularly useful
+ method, because translations do not make much sense out of the
+ context of their transcript. It may be better to use the
+ TranscriptAdaptor::fetch_all_by_external_name instead.
+ Returntype : reference to a list of Translations
Exceptions : none
- Caller : ?
+ Caller : general
=cut
-sub fetch_all_by_DBEntry {
+sub fetch_all_by_external_name {
my $self = shift;
my $external_id = shift;
- my @trans = ();
-
- my $entryAdaptor = $self->db->get_DBEntryAdaptor();
- my @ids = $entryAdaptor->translationids_by_extids($external_id);
- foreach my $trans_id ( @ids ) {
- my $trans = $self->fetch_by_dbID( $trans_id );
- warn $trans_id;
- if( $trans ) {
- push( @trans, $trans );
+
+ my $entry_adaptor = $self->db->get_DBEntryAdaptor();
+ my @ids = $entry_adaptor->list_translation_ids_by_extids($external_id);
+
+ my $transcript_adaptor = $self->db()->get_TranscriptAdaptor();
+
+ my @out;
+
+ foreach my $id (@ids) {
+ my $transcript = $transcript_adaptor->fetch_by_translation_id($id);
+ if($transcript) {
+ push @out, $self->fetch_by_Transcript($transcript);
}
- }
- return \@trans;
+ }
+
+ return \@out;
}
@@ -141,38 +181,49 @@ sub fetch_all_by_DBEntry {
The translation object to be stored in the database
Example : $transl_id = $translation_adaptor->store($translation);
Description: Stores a translation object in the database
- Returntype :
- Exceptions :
- Caller :
+ Returntype : int - the new dbID of the stored translation
+ Exceptions : thrown if the dbID of the start_Exon or end_Exon is not
+ defined.
+ thrown if only partial stable id information is present (e.g.
+ identifier but not version number)
+ Caller : Transcript::store
=cut
sub store {
- my ( $self, $translation ) = @_;
-
- unless( defined $translation->start_Exon->dbID &&
- defined $translation->end_Exon->dbID ) {
- $self->throw("Attempting to write a translation where the dbIDs of the " .
- "start and exons are not set. This is most likely to be " .
- "because you assigned the exons for translation start_exon " .
- "and translation end_exon to be different in memory " .
- "objects from your transcript exons - although it could " .
- "also be an internal error in the adaptors. For your " .
- "info the exon memory locations are " .
- $translation->start_Exon." and ".$translation->end_Exon());
+ my ( $self, $translation, $transcript_id ) = @_;
+
+ my $start_exon = $translation->start_Exon();
+ my $end_exon = $translation->end_Exon();
+
+ if(!$start_exon) {
+ throw("Translation must define a start_Exon to be stored.");
}
- my $sth = $self->prepare( "INSERT INTO translation( seq_start, start_exon_id,
- seq_end, end_exon_id)
- VALUES( ?,?,?,? )");
+ if(!$end_exon) {
+ throw("Translation must define an end_Exon to be stored.");
+ }
- $sth->execute( $translation->start(),
- $translation->start_Exon()->dbID(),
- $translation->end(),
- $translation->end_Exon()->dbID() );
+ if(!$start_exon->dbID) {
+ throw("start_Exon must have a dbID for Translation to be stored.");
+ }
- my $transl_dbID = $sth->{'mysql_insertid'};
+ if(!$end_exon->dbID) {
+ throw("end_Exon must have a dbID for Translation to be stored.");
+ }
+
+ my $sth = $self->prepare( "
+ INSERT INTO translation( seq_start, start_exon_id,
+ seq_end, end_exon_id, transcript_id)
+ VALUES ( ?,?,?,?,? )");
+ $sth->execute( $translation->start(),
+ $translation->start_Exon()->dbID(),
+ $translation->end(),
+ $translation->end_Exon()->dbID(),
+ $transcript_id );
+
+ my $transl_dbID = $sth->{'mysql_insertid'};
#store object xref mappings to translations
@@ -185,19 +236,18 @@ sub store {
if (defined($translation->stable_id)) {
if (!defined($translation->version)) {
- $self->throw("Trying to store incomplete stable id information for translation");
+ throw("Trying to store incomplete stable id information for translation");
}
- my $statement = "INSERT INTO translation_stable_id(translation_id," .
- "stable_id,version)".
- " VALUES(" . $transl_dbID . "," .
- "'" . $translation->stable_id . "'," .
- $translation->version .
- ")";
-
- my $sth = $self->prepare($statement);
- $sth->execute();
- }
+ my $sth = $self->prepare
+ ("INSERT INTO translation_stable_id(translation_id, stable_id, version)".
+ " VALUES (?, ?, ?)");
+
+ $sth->execute($transl_dbID, $translation->stable_id(),
+ $translation->version());
+
+ $sth->finish();
+ }
$translation->dbID( $transl_dbID );
$translation->adaptor( $self );
@@ -206,36 +256,6 @@ sub store {
}
-=head2 get_stable_entry_info
-
- Title : get_stable_entry_info
- Usage : $translationAdaptor->get_stable_entry_info($translation)
- Function: gets stable info for translation and places it into the hash
- Returns :
- Args :
-
-
-=cut
-
-sub get_stable_entry_info {
- my ($self,$translation) = @_;
-
- unless(defined $translation && ref $translation &&
- $translation->isa('Bio::EnsEMBL::Translation') ) {
- $self->throw("Needs a Translation object, not a [$translation]");
- }
-
- my $sth = $self->prepare("SELECT stable_id, version
- FROM translation_stable_id
- WHERE translation_id = ?");
- $sth->execute($translation->dbID());
-
- my @array = $sth->fetchrow_array();
- $translation->{'_stable_id'} = $array[0];
- $translation->{'_version'} = $array[1];
-
- return 1;
-}
sub remove {
@@ -248,9 +268,11 @@ sub remove {
$sth = $self->prepare("DELETE FROM translation_stable_id
WHERE translation_id = ?" );
$sth->execute( $translation->dbID );
- $translation->dbID( undef ); #don't think this line works
+ $translation->dbID( undef );
+ $translation->adaptor(undef);
}
+
=head2 list_dbIDs
Arg [1] : none
@@ -268,14 +290,16 @@ sub list_dbIDs {
return $self->_list_dbIDs("translation");
}
+
=head2 list_stable_dbIDs
Arg [1] : none
- Example : @stable_translation_ids = @{$translation_adaptor->list_stable_dbIDs()};
- Description: Gets an array of stable ids for all translations in the current db
- Returntype : list of ints
+ Example : @transl_stable_ids = @{$transl_adaptor->list_stable_dbIDs()};
+ Description: Gets an array of stable ids for all translations in the current
+ db
+ Returntype : reference to a list of strings
Exceptions : none
- Caller : ?
+ Caller : general
=cut
@@ -285,4 +309,121 @@ sub list_stable_ids {
return $self->_list_dbIDs("translation_stable_id", "stable_id");
}
+
+
+=head2 fetch_by_dbID
+
+ Arg [1] : int $dbID
+ The internal identifier of the Translation to obtain
+ Example : $translation = $translation_adaptor->fetch_by_dbID(1234);
+ Description: This fetches a Translation object via its internal id. This
+ is only debatably useful since translations do not make much
+ sense outside of the context of their Translation. Consider
+ using fetch_by_Transcript instead.
+ Returntype : Bio::EnsEMBL::Translation or undef if the translation is not
+ found.
+ Exceptions : warning if an additional (old style) Transcript argument is
+ provided
+ Caller : ?
+
+=cut
+
+sub fetch_by_dbID {
+ my ($self,$dbID, $transcript) = @_;
+
+ if($transcript) {
+ deprecate("Use of fetch_by_dbID with a Transcript argument is deprecated."
+ . "Use fetch_by_Transcript instead." );
+ }
+
+ if(!$dbID) {
+ throw("dbID argument is required");
+ }
+
+ my $transcript_adaptor = $self->db()->get_TranscriptAdaptor();
+ $transcript = $transcript_adaptor->fetch_by_translation_id($dbID);
+
+ return undef if(!$transcript);
+
+ return $self->fetch_by_Transcript($transcript);
+}
+
+
+=head2 fetch_by_dbID
+
+ Arg [1] : int $dbID
+ The internal identifier of the Translation to obtain
+ Example : $translation = $translation_adaptor->fetch_by_dbID(1234);
+ Description: This fetches a Translation object via its internal id. This
+ is only debatably useful since translations do not make much
+ sense outside of the context of their Translation. Consider
+ using fetch_by_Transcript instead.
+ Returntype : Bio::EnsEMBL::Translation or undef if the translation is not
+ found.
+ Exceptions : warning if an additional (old style) Transcript argument is
+ provided
+ Caller : ?
+
+=cut
+
+sub fetch_by_stable_id {
+ my ($self,$stable_id) = @_;
+
+ if(!$stable_id) {
+ throw("stable id argument is required");
+ }
+
+ my $transcript_adaptor = $self->db()->get_TranscriptAdaptor();
+ my $transcript =
+ $transcript_adaptor->fetch_by_translation_stable_id($stable_id);
+
+ return undef if(!$transcript);
+
+ return $self->fetch_by_Transcript($transcript);
+}
+
+
+
+
+=head2 fetch_all_by_DBEntry
+
+ Description: DEPRECATED, this has been renames fetch_all_by_external_name
+
+=cut
+
+sub fetch_all_by_DBEntry {
+ my $self = shift;
+ deprecate("Use fetch_all_by_external_name instead.");
+ return $self->fetch_all_by_external_name(@_);
+}
+
+=head2 get_stable_entry_info
+
+ Description: DEPRECATED - This method should no longer be needed. Stable
+ id info is fetched when the transcript is.
+
+=cut
+
+sub get_stable_entry_info {
+ my ($self,$translation) = @_;
+
+ deprecate( "This method shouldnt be necessary any more" );
+
+ unless(defined $translation && ref $translation &&
+ $translation->isa('Bio::EnsEMBL::Translation') ) {
+ throw("Needs a Translation object, not a [$translation]");
+ }
+
+ my $sth = $self->prepare("SELECT stable_id, version
+ FROM translation_stable_id
+ WHERE translation_id = ?");
+ $sth->execute($translation->dbID());
+
+ my @array = $sth->fetchrow_array();
+ $translation->{'_stable_id'} = $array[0];
+ $translation->{'_version'} = $array[1];
+
+ return 1;
+}
+
1;
diff --git a/modules/Bio/EnsEMBL/DensityFeature.pm b/modules/Bio/EnsEMBL/DensityFeature.pm
new file mode 100644
index 0000000000..38335c7504
--- /dev/null
+++ b/modules/Bio/EnsEMBL/DensityFeature.pm
@@ -0,0 +1,191 @@
+#
+# Ensembl module for Bio::EnsEMBL::DensityFeature
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::DensityFeature - A feature representing a density, or precentage
+coverage etc. in a given region.
+
+=head1 SYNOPSIS
+
+use Bio::EnsEMBL::DensityFeature;
+
+$feature = Bio::EnsEMBL::DensityFeature->new(-start => 100,
+ -end => 220,
+ -slice => $slice,
+ -analysis => $analysis,
+ -density_value => 90.1,
+ -density_value_type => 'sum',
+ -dbID => 112,
+ -adaptor => $adaptor);
+
+=head1 DESCRIPTION
+
+A density feature represents a count, density, or percentage coverage, etc. for
+a given region.
+
+This module is part of the Ensembl project http://www.ensembl.org
+
+=head1 CONTACT
+
+Post comments/questions to the ensembl development list: ensembl-dev@ebi.ac.uk
+
+=head1 METHODS
+
+=cut
+
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::DensityFeature;
+
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(throw);
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::Feature);
+
+
+=head2 new
+
+ Arg [DENSITY_VALUE] : The number of features which were found within the
+ region of this DensityFeature. May also be a percentage or
+ coverage, etc.
+ Arg [DENSITY_VALUE_TYPE] : string - should be 'ratio' or 'sum'. A value
+ which is a sum represents a summation values in the
+ the range of this feature. A ratio is some sort of average
+ of the values in the range of this feature. A 'sum' could be
+ a count of the snps in the region and a 'ratio' could be a
+ percent gc contenct or repeat coverage.
+ Arg [...] : Named arguments passed to superclass
+ Example : $feature = Bio::EnsEMBL::SimpleFeature->new
+ (-start => 1,
+ -end => 1e6,
+ -analysis => $analysis,
+ -density_value => 80.5,
+ -density_value_type => 'ratio');
+ Description: Creates a new density feature.
+ Returntype : Bio::EnsEMBL::DensityFeature
+ Exceptions : throw if invalid density value type is provided
+ Caller : general
+
+=cut
+
+sub new {
+ my $caller = shift;
+
+ #allow constructor to be called as class or object method
+ my $class = ref($caller) || $caller;
+
+ my $self = $class->SUPER::new(@_);
+
+ my($density_value,$density_value_type) =
+ rearrange(['DENSITY_VALUE', 'DENSITY_VALUE_TYPE'], @_);
+
+ throw("Density value must be >= 0.") if($density_value < 0);
+
+ if($density_value) {
+ $density_value = lc($density_value);
+ if($density_value_type ne 'sum' && $density_value_type ne 'ratio') {
+ throw("Unknown density value type [$density_value_type].");
+ }
+ }
+
+ $self->{'density_value'} = $density_value;
+ $self->{'density_value_type'} = $density_value_type;
+ $self->{'strand'} = 0;
+
+ return $self;
+}
+
+
+sub new_fast {
+ my $class = shift;
+ my $hashref = shift;
+ return bless($hashref,$class);
+}
+
+
+=head2 strand
+
+ Arg [1] : none
+ Example : $strand = $df->strand();
+ Description: Getter fot the strand attribute. Density features always have
+ strand 0 and this attribute is not settable.
+ Returntype : int (always 0)
+ Exceptions : warning if an attempt is made to set the strand
+ Caller : general
+
+=cut
+
+sub strand {
+ my $self = shift;
+ warning("DensityFeature strand is not settable") if(@_);
+ return 0;
+}
+
+
+
+=head2 density_value
+
+ Arg [1] : (optional) float $density_value
+ Example : $dv = $density_feature->density_value();
+ Description: Getter/Setter for the density value of this DensityFeature.
+ The density value may be a count, a percentage, or a coverage
+ of a feature type in the area defined by this feature.
+ Returntype : float
+ Exceptions : throw if a negative density value is provided
+ Caller : general
+
+=cut
+
+sub density_value {
+ my $self = shift;
+
+ if(@_) {
+ my $density_value = shift;
+ throw("Density value must be >= 0.") if($density_value < 0);
+ $self->{'density_value'} = $density_value;
+ }
+
+ return $self->{'density_value'};
+}
+
+
+
+
+=head2 density_value_type
+
+ Arg [1] : string $newval (optional)
+ The new value to set the density_value_type attribute to
+ Example : $density_value_type = $obj->density_value_type()
+ Description: Getter/Setter for the density_value_type attribute
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub density_value_type{
+ my $self = shift;
+ if(@_) {
+ my $density_value_type = lc(shift);
+ if($density_value_type ne 'sum' && $density_value_type ne 'ratio') {
+ throw("Unknown density value type [$density_value_type]");
+ }
+ $self->{'density_value_type'} = $density_value_type;
+ }
+ return $self->{'density_value_type'};
+}
+
+1;
+
+
+
diff --git a/modules/Bio/EnsEMBL/Exon.pm b/modules/Bio/EnsEMBL/Exon.pm
index 4e9d761104..13bb15aef8 100755
--- a/modules/Bio/EnsEMBL/Exon.pm
+++ b/modules/Bio/EnsEMBL/Exon.pm
@@ -1,7 +1,6 @@
#
-# BioPerl module for Exon
+# EnsEMBL module for Bio::EnsEMBL::Exon
#
-# Cared for by Ewan Birney <birney@sanger.ac.uk>
#
# Copyright Ewan Birney
#
@@ -11,40 +10,35 @@
=pod
-=head1 NAME
-
-Bio::EnsEMBL::Exon - Confirmed Exon
+=head1 NAME Bio::EnsEMBL::Exon - A class representing an Exon
=head1 SYNOPSIS
- $ex = new Bio::EnsEMBL::Exon;
-
- $ex->start(10);
- $ex->end(100);
-
-Examples of creating an exon
-
- # start = 1208, end = 1506, forward strand
- $ex = new Bio::EnsEMBL::Exon(1208,1506,1)
-
- Start and end coordinates are always stored with start < end. If they are
- input in the reverse order they will be swapped over. The value for the
- strand will be kept as its input value;
-
- Strand values: + or 1 = forward strand
- - or -1 = reverse strand
- . or 0 = unknown strand
+ $ex = new Bio::EnsEMBL::Exon(-START => 100,
+ -END => 200,
+ -STRAND => 1,
+ -SLICE => $slice,
+ -DBID => $dbID,
+ -ANALYSIS => $analysis,
+ -STABLE_ID => 'ENSE000000123',
+ -VERSION => 2
+ );
- $ex->contig($dna); # $dna is a Bio::Seq
- $ex->phase(0); # Sets the phase of the exon
- $ex->end_phase(1); # sets the end_phase of the exon
+ #seq returns a Bio::Seq
+ my $seq = $exon->seq->seq();
- Phase values are 0,1,2
+ #peptide only makes sense within transcript context
+ my $pep = $exon->peptide($transcript)->seq();
+ #normal feature operations can be performed:
+ $exon = $exon->transform('clone');
+ $exon->move($new_start, $new_end, $new_strand);
+ print $exon->slice->seq_region_name();
=head1 DESCRIPTION
-Exon object.
+This is a class which represents an exon which is part of a transcript.
+See Bio::EnsEMBL:Transcript
=head1 CONTACT
@@ -52,27 +46,24 @@ Post questions to the EnsEMBL developer list: <ensembl-dev@ebi.ac.uk>
=head1 APPENDIX
-The rest of the documentation details each of the object methods. Internal
+The rest of the documentation details each of the object methods. Internal
methods are usually preceded with a_
=cut
-
-# Let the code begin...
-
-
package Bio::EnsEMBL::Exon;
-use vars qw(@ISA $AUTOLOAD);
+use vars qw(@ISA);
use strict;
-# Object preamble - inherits from Bio::SeqFeature::Generic
-use Bio::EnsEMBL::SeqFeature;
+use Bio::EnsEMBL::Feature;
use Bio::Seq; # exons have to have sequences...
-use Bio::EnsEMBL::StickyExon;
-@ISA = qw(Bio::EnsEMBL::SeqFeature);
+use Bio::EnsEMBL::Utils::Exception qw( warning throw deprecate );
+use Bio::EnsEMBL::Utils::Argument qw( rearrange );
+
+@ISA = qw(Bio::EnsEMBL::Feature);
=head2 new
@@ -87,22 +78,28 @@ use Bio::EnsEMBL::StickyExon;
=cut
sub new {
- my($class,@args) = @_;
+ my $class = shift;
- my $self = $class->SUPER::new(@args);
-
- # set exon rank to be 1 be default
- $self->sticky_rank(1);
+ $class = ref $class || $class;
+
+ my $self = $class->SUPER::new( @_ );
+
+ my ( $phase, $end_phase, $stable_id, $version ) =
+ rearrange( [ "PHASE", "END_PHASE", "STABLE_ID", "VERSION" ], @_ );
- # set stuff in self from @args
- return $self; # success - we hope!
+ $self->{'phase'} = $phase;
+ $self->{'end_phase'} = $end_phase;
+ $self->{'stable_id'} = $stable_id;
+ $self->{'version'} = $version;
+
+ return $self;
}
=head2 new_fast
- Arg [1] : Bio::EnsEMBL::RawContig/Bio::EnsEMBL::Slice $contig
+ Arg [1] : Bio::EnsEMBL::Slice $slice
Arg [2] : int $start
Arg [3] : int $end
Arg [4] : int $strand (1 or -1)
@@ -115,7 +112,7 @@ sub new {
=cut
sub new_fast {
- my ($class,$contig,$start,$end,$strand) = @_;
+ my ($class,$slice,$start,$end,$strand) = @_;
my $self = bless {}, $class;
@@ -123,465 +120,19 @@ sub new_fast {
# We assume that the strand is correct and keep the input value.
if ($start > $end) {
- my $tmp = $end;
- $end = $start;
- $start = $tmp;
+ throw( "End smaller than start not allowed" );
}
-
+
$self->start ($start);
$self->end ($end);
$self->strand($strand);
- $self->contig($contig);
-
- return $self;
-}
-
-
-
-=head2 dbID
-
- Arg [1] : int $dbID
- Example : none
- Description: get/set for the database internal id
- Returntype : int
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub dbID {
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'dbID'} = $value;
- }
- return $self->{'dbID'};
-
-}
-
-
-
-=head2 temporary_id
-
- Arg [1] : string $temporary_id
- Example : none
- Description: get/set for attribute temporary_id
- was invented from genebuild and shouldnt be necessary
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-
-sub temporary_id {
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'tempID'} = $value;
- }
- return $self->{'tempID'};
-
-}
-
-
-=head2 adaptor
-
- Arg [1] : Bio::EnsEMBL::DBSQL::ExonAdaptor $adaptor
- Example : none
- Description: get/set for this objects Adaptor
- Returntype : Bio::EnsEMBL::DBSQL::ExonAdaptor
- Exceptions : none
- Caller : general, set from adaptor on store
-
-=cut
-
-sub adaptor {
- my $self = shift;
- if( @_ ) {
- $self->{'adaptor'} = shift;
- }
- return $self->{'adaptor'};
-
-}
-
-
-
-=head2 _transform_between_Slices
-
- Arg [1] : Bio::EnsEMBL::Slice $new_slice
- Example : none
- Description: Transforms the exons from one Slice to the given Slice,
- that needs to be on the same Chromosome. The method overwrites
- the same method in Bio::EnsEMBL::SeqFeature
- Returntype : Bio::EnsEMBL::Exon
- Exceptions : Checks if Slice is attached and argument is Slice on same
- chromosome.
- Caller : transform
-
-=cut
-
-sub _transform_between_Slices {
- my ($self, $to_slice) = @_;
-
- my $from_slice = $self->contig();
-
- #sanity check - make sure we have something to transform from
- unless(defined $from_slice) {
- $self->throw("Exon's contig is not defined - cannot transform between "
- . "slices\n");
- }
- #sanity check - make sure the from slice's chromosome is defined
- unless(defined $from_slice->chr_name()) {
- $self->throw("Exon's chromosome is not defined - cannot transform between "
- . "slices\n");
- }
-
- unless(defined $to_slice->chr_name()) {
- #sanity check - we need an adaptor from a slice
- my $slice_adaptor = $to_slice->adaptor || $from_slice->adaptor;
- unless($slice_adaptor) {
- $self->throw("Exon cannot be transformed to empty slice without an " .
- "an attached adaptor on the From slice or To slice");
- }
- #from slice is an empty slice, create a entire chromosome slice
- %$to_slice = %{$slice_adaptor->fetch_by_chr_name($from_slice->chr_name())};
- }
-
- #sanity check - make sure we are transforming to the same chromosome
- if($to_slice->chr_name() ne $from_slice->chr_name()) {
- $self->throw("Cannot transform exon on chr " . $from_slice->chr_name() .
- "to chr " . $to_slice->chr_name());
- }
-
- #create a copy of the old exon
- my $new_exon = new Bio::EnsEMBL::Exon;
- %$new_exon = %$self;
-
- #unset the new exons supporting features
- delete $new_exon->{'_supporting_evidence'};
-
- #calculate the exons position in the assembly
- my ($exon_chr_start, $exon_chr_end, $exon_chr_strand);
- if($from_slice->strand == 1) {
- $exon_chr_start = $self->start + $from_slice->chr_start - 1;
- $exon_chr_end = $self->end + $from_slice->chr_start - 1;
- $exon_chr_strand = $self->strand;
- } else {
- $exon_chr_start = $from_slice->chr_end - $self->end + 1;
- $exon_chr_end = $from_slice->chr_end - $self->start + 1;
- $exon_chr_strand = $self->strand * -1;
- }
-
- #now calculate the exons position on the new slice
- if($to_slice->strand == 1) {
- $new_exon->start($exon_chr_start - $to_slice->chr_start + 1);
- $new_exon->end ($exon_chr_end - $to_slice->chr_start + 1);
- $new_exon->strand($exon_chr_strand);
- } else {
- $new_exon->start($to_slice->chr_end - $exon_chr_end + 1);
- $new_exon->end ($to_slice->chr_end - $exon_chr_start + 1);
- $new_exon->strand($exon_chr_strand * -1);
- }
-
- $new_exon->contig($to_slice);
-
- #copy the attached supporting features and transform them
- my @feats;
- if( exists $self->{_supporting_evidence} ) {
- foreach my $sf (@{$self->get_all_supporting_features()}) {
- #my $f = $sf->new();
- #%$f = %$sf;
- ###(mcvicker) this would be better if the feature was copied
- push @feats, $sf->transform($to_slice);
- }
- $new_exon->add_supporting_features(@feats);
- }
-
- return $new_exon;
-}
-
-
-
-=head2 _transform_to_Slice
-
- Arg [1] : Bio::EnsEMBL::Slice $slice
- Example : none
- Description: Transforms this Exon from RawContig coord to given Slice coord.
- The method overrides the same method in Bio::EnsEMBL::SeqFeature
- Returntype : Bio::EnsEMBL::Exon
- Exceptions : If the RawContig coords dont map
- Caller : transform
-
-=cut
-
-sub _transform_to_Slice {
- my ($self,$slice) = @_;
-
- unless($self->contig) {
- $self->throw("Exon's contig must be defined to transform to Slice coords");
- }
- #print STDERR "transforming ".$self." from raw contig to slice coords\n";
- #print STDERR "exon ".$self->stable_id." ".$self->gffstring."\n";
- my $adaptor = $slice->adaptor || $self->contig->adaptor;
-
- unless($adaptor) {
- $self->throw("Cannot transform to exon slice unless either the " .
- "exon->contig->adaptor or slice->adaptor is defined");
- }
-
- my $mapper = $adaptor->db->get_AssemblyMapperAdaptor->fetch_by_type
- ( $slice->assembly_type() );
-
- my @mapped = $mapper->map_coordinates_to_assembly
- (
- $self->contig()->dbID,
- $self->start(),
- $self->end(),
- $self->strand()
- );
-
- # exons should always transform so in theory no error check necessary
- # actually we could have exons inside and outside the Slice
- # because of db design and the query that produces them
- if( ! @mapped ) {
- $self->throw( "Exon couldnt map" );
- }
- # should get a gap object returned if an exon lies outside of
- # the current slice. Simply return the exon as is - i.e. untransformed.
- # this untransformed exon will be distinguishable as it will still have
- # contig attached to it and not a slice.
- if( $mapped[0]->isa( "Bio::EnsEMBL::Mapper::Gap" )) {
- return $self;
- }
-
- # the slice is an empty slice, create an enitre chromosome slice and
- # replace the empty slice with it
- if( ! defined $slice->chr_name() ) {
- my $slice_adaptor = $adaptor->db->get_SliceAdaptor;
- %$slice = %{$slice_adaptor->fetch_by_chr_name( $mapped[0]->id() )};
- }
-
- my $newexon = new Bio::EnsEMBL::Exon();
- %$newexon = %$self;
-
- #unset supporting features of new exon
- delete $newexon->{'_supporting_evidence'};
-
- if ($slice->strand == 1) {
- $newexon->start( $mapped[0]->start() - $slice->chr_start() + 1);
- $newexon->end( $mapped[0]->end() - $slice->chr_start() + 1);
- } else {
- $newexon->start( $slice->chr_end() - $mapped[0]->end() + 1);
- $newexon->end( $slice->chr_end() - $mapped[0]->start() + 1);
- }
-
- $newexon->strand( $mapped[0]->strand() * $slice->strand() );
- $newexon->contig( $slice );
- #copy the attached supporting features and transform them
- my @feats;
- if( exists $self->{_supporting_evidence} ) {
- foreach my $sf (@{$self->get_all_supporting_features()}) {
- #my $f = $sf->new();
- #%$f = %$sf;
- #(mcvicker) this would be better if the feature was copied
- push @feats, $sf->transform($slice);
- }
- $newexon->add_supporting_features(@feats);
- }
- #print STDERR "transformed exon ".$newexon->stable_id." ".$newexon->gffstring."\n";
- return $newexon;
-}
-
-
-
-=head2 _transform_to_RawContig
-
- Args : none
- Example : none
- Description: Transform this Exon from Slice to RawContig coords
- Returntype : Bio::EnsEMBL::Exon
- Exceptions : Exon cant lie on Gap
- Caller : transform
-
-=cut
-
-sub _transform_to_RawContig {
- my $self = shift;
-
- my $slice_adaptor = $self->contig->adaptor;
-
- unless($slice_adaptor) {
- $self->throw("Cannot transform exon to raw contig unless attached slice" .
- " has adaptor defined. (i.e. exon->contig->adaptor)");
- }
-
- my $asma = $slice_adaptor->db->get_AssemblyMapperAdaptor();
-
- my $mapper = $asma->fetch_by_type( $self->contig()->assembly_type() );
- my $rcAdaptor = $slice_adaptor->db->get_RawContigAdaptor();
- my $slice_chr_start = $self->contig->chr_start();
- my $slice_chr_end = $self->contig->chr_end();
-
- my ($exon_chr_start,$exon_chr_end);
-
- if ($self->contig()->strand() == 1) {
- $exon_chr_start = $self->start() + $slice_chr_start - 1;
- $exon_chr_end = $self->end() + $slice_chr_start - 1;
- }
- else {
- $exon_chr_end = $slice_chr_end - $self->start() + 1;
- $exon_chr_start = $slice_chr_end - $self->end() + 1;
- }
-
- my @mapped = $mapper->map_coordinates_to_rawcontig
- (
- $self->contig()->chr_name(),
- $exon_chr_start,
- $exon_chr_end,
- $self->strand()*$self->contig()->strand()
- );
-
- if( ! @mapped ) {
- $self->throw( "Exon couldnt map" );
- return $self;
- }
-
- #transform the supporting features to raw contig coords (hashed on contig)
- my %sf_hash;
-
- if( exists $self->{_supporting_evidence} ) {
- my $sfs = $self->get_all_supporting_features();
- SUPPORTING:foreach my $sf (@$sfs) {
- my @mapped_feats;
- eval{
- @mapped_feats = $sf->transform;
- };
- if($@){
- $self->warn("Supporting feature didn't mapped ignoring $@");
- next SUPPORTING;
- }
- foreach my $mapped_feat (@mapped_feats) {
- unless(exists $sf_hash{$mapped_feat->contig->name}) {
- $sf_hash{$mapped_feat->contig->name} = [];
- }
- push @{$sf_hash{$mapped_feat->contig->name}}, $mapped_feat;
- }
- }
- }
-
- if( scalar( @mapped ) > 1 ) {
- # sticky exons
- # bjeacchh
-
- my $stickyExon = Bio::EnsEMBL::StickyExon->new();
- $stickyExon->phase( $self->phase() );
- $stickyExon->end_phase($self->end_phase());
- $stickyExon->adaptor( $self->adaptor() );
- $stickyExon->start( 1 );
- if( defined $self->dbID() ) {
- $stickyExon->dbID( $self->dbID() );
- }
-
- my $sticky_length =0;
- # and then all the component exons ...
- for( my $i=0; $i <= $#mapped; $i++ ) {
- if($mapped[$i]->isa("Bio::EnsEMBL::Mapper::Gap")){
- $self->throw(" exon lies on a gap cannot be mapped\n");
- }
- my $componentExon = Bio::EnsEMBL::Exon->new();
- $componentExon->start( $mapped[$i]->start() );
- $componentExon->end( $mapped[$i]->end() );
- $componentExon->strand( $mapped[$i]->strand() );
- my $rawContig = $rcAdaptor->fetch_by_dbID( $mapped[$i]->id() );
- $componentExon->contig( $rawContig );
- $componentExon->sticky_rank( $i + 1 );
- $componentExon->phase( $self->phase );
- $componentExon->end_phase($self->end_phase);
- $componentExon->dbID( $self->dbID() );
- $componentExon->adaptor( $self->adaptor() );
-
- #add the supporting features on this contig to the component exon
- if(exists $sf_hash{$rawContig->name}) {
- $componentExon->add_supporting_features(@{$sf_hash{$rawContig->name}});
- }
-
- $stickyExon->add_component_Exon( $componentExon );
- $sticky_length += ( $mapped[$i]->end() - $mapped[$i]->start() + 1 );
- }
- $stickyExon->end( $sticky_length );
- $stickyExon->strand( 1 );
- if (defined($self->stable_id)) {
- $stickyExon->stable_id($self->stable_id);
- }
- if (defined($self->version)) {
- $stickyExon->version($self->version);
- }
- if (defined($self->created)) {
- $stickyExon->created($self->created);
- }
- if (defined($self->modified)) {
- $stickyExon->modified($self->modified);
- }
- return $stickyExon;
-
- } else {
- # thats a simple exon
- if($mapped[0]->isa("Bio::EnsEMBL::Mapper::Gap")){
-
- $self->throw(" exon lies on a gap cannot be mapped\n");
- }
- my $rawContig = $rcAdaptor->fetch_by_dbID( $mapped[0]->id() );
- my $new_exon = new Bio::EnsEMBL::Exon();
-
- #copy this exon
- %$new_exon = %$self;
-
- #unset supporting evidence of new exon
- delete $new_exon->{'_supporting_evidence'};
+ $self->slice($slice);
- $new_exon->start( $mapped[0]->start() );
- $new_exon->end( $mapped[0]->end() );
- $new_exon->strand( $mapped[0]->strand() );
- # attach raw contig
- $new_exon->contig( $rawContig );
-
- #replace old supporting feats with transformed supporting feats
- $new_exon->add_supporting_features(@{$sf_hash{$rawContig->name}});
- #print STDERR "transformed exon ".$new_exon->gffstring."\n";
- return $new_exon;
-
- }
+ return $self;
}
-=head2 sticky_rank
-
- Arg [1] : (optional) int $value
- Example : $sticky_rank = $exon->sticky_rank
- Description: Returns the position of a component exon in its assembled sticky
- exon. Normal exons all have sticky_rank = 1 as do exons in
- slice coordinates. Exons in RawContig coordinates which span
- multiple contigs are 'sticky' and are made of component exons
- whose order are denoted by this attribute.
- Returntype : int
- Exceptions : none
- Caller : Bio::EnsEMBL::StickyExon
-
-=cut
-
-sub sticky_rank{
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'sticky_rank'} = $value;
- }
- return $obj->{'sticky_rank'};
-
-}
-
-
=head2 end_phase
@@ -601,19 +152,20 @@ sub sticky_rank{
=cut
sub end_phase {
- my ($self,$endphase) = @_;
- if ( defined($endphase) ){
- $self->{_end_phase} = $endphase;
- }
- if ( !defined( $self->{_end_phase} ) ){
- $self->throw("No end phase set in Exon. You must set it explicitly. $!" .
- "Caller: ".caller);
+ my $self = shift;
+ if( @_ ) {
+ $self->{'end_phase'} = shift;
+ } else {
+ if( ! defined ( $self->{'end_phase'} )) {
+ warning( "No end phase set in Exon. You must set it explicitly." );
+ }
}
- return $self->{_end_phase};
+ return $self->{'end_phase'};
}
+
=pod
=head2 phase
@@ -659,8 +211,8 @@ sub phase {
#print STDERR "Setting phase to $value\n";
$self->{'phase'} = $value;
} else {
- $self->throw("Bad value ($value) for exon phase. Should only be" .
- " -1,0,1,2\n");
+ throw("Bad value ($value) for exon phase. Should only be" .
+ " -1,0,1,2\n");
}
}
return $self->{'phase'};
@@ -684,7 +236,7 @@ sub frame {
my ($self,$value) = @_;
if( defined $value ) {
- $self->throw("Cannot set frame. Deduced from seq_start and phase");
+ throw("Cannot set frame. Deduced from seq_start and phase");
}
# frame is mod 3 of the translation point
@@ -704,34 +256,220 @@ sub frame {
return ($self->start+1)%3;
}
- $self->throw("bad phase in exon ".$self->phase);
+ throw("bad phase in exon ".$self->phase);
+
+}
+
+
+
+=head2 type
+
+ Arg [1] : (optional) $value
+ Example : Gets/Sets th etype of this exon
+ Description: Returns the type of the exon (Init, Intr, Term)
+ Returntype : string
+ Exceptions : none
+ Caller : ?
+
+=cut
+
+sub type {
+ my ($self,$value) = @_;
+
+ if (defined($value)) {
+ $self->{'type'} = $value;
+ }
+ return $self->{'type'};
+}
+
+
+
+=head2 start
+
+ Arg [1] : int $start (optional)
+ Example : $start = $exon->start();
+ Description: Getter/Setter for the start of this exon. The superclass
+ implmentation is overridden to flush the internal sequence
+ cache if this value is altered
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub start {
+ my $self = shift;
+ #if an arg was provided, flush the internal sequence cache
+ delete $self->{'_seq_cache'} if(@_);
+ return $self->SUPER::start(@_);
+}
+
+
+=head2 end
+
+ Arg [1] : int $end (optional)
+ Example : $end = $exon->end();
+ Description: Getter/Setter for the end of this exon. The superclass
+ implmentation is overridden to flush the internal sequence
+ cache if this value is altered
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub end {
+ my $self = shift;
+ #if an arg was provided, flush the internal sequence cache
+ delete $self->{'_seq_cache'} if(@_);
+ return $self->SUPER::end(@_);
+}
+
+
+=head2 strand
+
+ Arg [1] : int $strand (optional)
+ Example : $start = $exon->strand();
+ Description: Getter/Setter for the strand of this exon. The superclass
+ implmentation is overridden to flush the internal sequence
+ cache if this value is altered
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub strand {
+ my $self = shift;
+ #if an arg was provided, flush the internal sequence cache
+ delete $self->{'_seq_cache'} if(@_);
+ return $self->SUPER::strand(@_);
+}
+
+
+=head2 slice
+
+ Arg [1] : Bio::EnsEMBL::Slice
+ Example : $slice = $exon->slice();
+ Description: Getter/Setter for the slice this exon is on. The superclass
+ implmentation is overridden to flush the internal sequence
+ cache if this value is altered
+ Returntype : Bio::EnsEMBL::Slice
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub contig {
+ my $self = shift;
+ #if an arg was provided, flush the internal sequence cache
+ delete $self->{'_seq_cache'} if(@_);
+ return $self->SUPER::slice(@_);
+}
+
+
+
+=head2 move
+
+ Arg [1] : int start
+ Arg [2] : int end
+ Arg [3] : (optional) int strand
+ Example : None
+ Description: Sets the start, end and strand in one call rather than in
+ 3 seperate calls to the start(), end() and strand() methods.
+ This is for convenience and for speed when this needs to be
+ done within a tight loop. This overrides the superclass
+ move() method so that the internal sequence cache can be
+ flushed if the exon if moved.
+ Returntype : none
+ Exceptions : Thrown is invalid arguments are provided
+ Caller : general
+
+=cut
+
+sub move {
+ my $self = shift;
+ #flush the internal sequence cache
+ delete $self->{'_seq_cache'};
+ return $self->SUPER::move(@_);
+}
+
+
+
+=head2 transform
+
+ Arg 1 : String $coordinate_system_name
+ Arg [2] : String $coordinate_system_version
+ Description: moves this exon to the given coordinate system. If this exon has
+ attached supporting evidence, they move as well.
+ Returntype : Bio::EnsEMBL::Exon
+ Exceptions : wrong parameters
+ Caller : general
+
+=cut
+
+
+sub transform {
+ my $self = shift;
+
+ # catch for old style transform calls
+ if( !@_ || ( ref $_[0] && $_[0]->isa( "Bio::EnsEMBL::Slice" ))) {
+ deprecate('Calling transform without a coord system name is deprecated.');
+ return $self->_deprecated_transform(@_);
+ }
+
+ my $new_exon = $self->SUPER::transform( @_ );
+ return undef unless $new_exon;
+
+ if( exists $self->{'_supporting_evidence'} ) {
+ my @new_features;
+ for my $old_feature ( @{$self->{'_supporting_evidence'}} ) {
+ my $new_feature = $old_feature->transform( @_ );
+ push( @new_features, $new_feature );
+ }
+ $new_exon->{'_supporting_evidence'} = \@new_features;
+ }
+
+ #dont want to share the same sequence cache
+ delete $new_exon->{'_seq_cache'};
+ return $new_exon;
}
-=head2 type
+=head2 transfer
- Arg [1] : (optional) $value
- Example : Gets/Sets th etype of this exon
- Description: Returns the type of the exon (Init, Intr, Term)
- Returntype : string
+ Arg [1] : Bio::EnsEMBL::Slice $destination_slice
+ Example : none
+ Description: Moves this Exon to given target slice coordinates. If Features
+ are attached they are moved as well. Returns a new exon.
+ Returntype : Bio::EnsEMBL::Gene
Exceptions : none
- Caller : ?
+ Caller : general
=cut
-sub type {
- my ($self,$value) = @_;
-
- if (defined($value)) {
- $self->{'type'} = $value;
- }
- return $self->{'type'};
-}
+sub transfer {
+ my $self = shift;
+ my $new_exon = $self->SUPER::transfer( @_ );
+ return undef unless $new_exon;
+
+ if( exists $self->{'_supporting_evidence'} ) {
+ my @new_features;
+ for my $old_feature ( @{$self->{'_supporting_evidence'}} ) {
+ my $new_feature = $old_feature->transfer( @_ );
+ push( @new_features, $new_feature );
+ }
+ $new_exon->{'_supporting_evidence'} = \@new_features;
+ }
+ #dont want to share the same sequence cache
+ delete $new_exon->{'_seq_cache'};
+ return $new_exon;
+}
@@ -758,22 +496,21 @@ sub add_supporting_features {
#print STDERR "calling add supporting features\n\n";
return unless @features;
- $self->{_supporting_evidence} = []
- unless defined($self->{_supporting_evidence});
+ $self->{_supporting_evidence} ||= [];
# check whether this feature object has been added already
FEATURE: foreach my $feature (@features) {
#print STDERR "have ".$feature." to add to exon\n\n";
- unless($feature && $feature->isa("Bio::EnsEMBL::SeqFeatureI")) {
- $self->throw("Supporting feat [$feature] not a " .
- "Bio::EnsEMBL::SeqFeatureI");
+ unless($feature && $feature->isa("Bio::EnsEMBL::Feature")) {
+ throw("Supporting feat [$feature] not a " .
+ "Bio::EnsEMBL::Feature");
}
- if ((defined $self->contig() && defined $feature->contig())&&
- ( $self->contig()->name() ne $feature->contig()->name())){
- $self->throw("Supporting feat not in same coord system as exon\n" .
- "exon is attached to [".$self->contig->name()."]\n" .
- "feat is attached to [".$feature->contig->name()."]");
+ if ((defined $self->slice() && defined $feature->slice())&&
+ ( $self->slice()->name() ne $feature->slice()->name())){
+ throw("Supporting feat not in same coord system as exon\n" .
+ "exon is attached to [".$self->slice()->name()."]\n" .
+ "feat is attached to [".$feature->slice()->name()."]");
}
foreach my $added_feature ( @{ $self->{_supporting_evidence} } ){
@@ -838,7 +575,7 @@ sub get_all_supporting_features {
sub find_supporting_evidence {
my ($self,$features,$sorted) = @_;
-
+
foreach my $f (@$features) {
# return if we have a sorted feature array
if ($sorted == 1 && $f->start > $self->end) {
@@ -859,221 +596,39 @@ sub find_supporting_evidence {
}
}
+=head2 stable_id
-
-
-=head2 start
-
- Arg [1] : int $start (optional)
- Example : $start = $exon->start();
- Description: Getter/Setter for the start of this exon. The superclass
- implmentation is overridden to flush the internal sequence
- cache if this value is altered
- Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub start {
- my $self = shift;
- #if an arg was provided, flush the internal sequence cache
- delete $self->{'_seq_cache'} if(@_);
- return $self->SUPER::start(@_);
-}
-
-
-=head2 end
-
- Arg [1] : int $end (optional)
- Example : $end = $exon->end();
- Description: Getter/Setter for the end of this exon. The superclass
- implmentation is overridden to flush the internal sequence
- cache if this value is altered
- Returntype : int
+ Arg [1] : string $stable_id
+ Example : none
+ Description: get/set for attribute stable_id
+ Returntype : string
Exceptions : none
Caller : general
=cut
-sub end {
- my $self = shift;
- #if an arg was provided, flush the internal sequence cache
- delete $self->{'_seq_cache'} if(@_);
- return $self->SUPER::end(@_);
+sub stable_id {
+ my $self = shift;
+ $self->{'stable_id'} = shift if( @_ );
+ return $self->{'stable_id'};
}
-=head2 strand
-
- Arg [1] : int $strand (optional)
- Example : $start = $exon->strand();
- Description: Getter/Setter for the strand of this exon. The superclass
- implmentation is overridden to flush the internal sequence
- cache if this value is altered
- Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub strand {
- my $self = shift;
- #if an arg was provided, flush the internal sequence cache
- delete $self->{'_seq_cache'} if(@_);
- return $self->SUPER::strand(@_);
-}
-
-=head2 contig
+=head2 version
- Arg [1] : Bio::EnsEMBL::Slice or Bio::EnsEMBL::RawContig (optional)
- Example : $slice = $exon->contig();
- Description: Getter/Setter for the contig of this exon. The superclass
- implmentation is overridden to flush the internal sequence
- cache if this value is altered
- Returntype : int
+ Arg [1] : string $version
+ Example : none
+ Description: get/set for attribute version
+ Returntype : string
Exceptions : none
Caller : general
=cut
-sub contig {
- my $self = shift;
- #if an arg was provided, flush the internal sequence cache
- delete $self->{'_seq_cache'} if(@_);
- return $self->SUPER::contig(@_);
-}
-
-
-
-
-=head2 created
-
- Title : created
- Usage : $obj->created()
- Function:
- Returns : value of created
- Args :
-
-
-=cut
-
-sub created{
- my ($self,$value) = @_;
-
- if(defined $value ) {
- $self->{'_created'} = $value;
- }
-
-
- if( exists $self->{'_created'} ) {
- return $self->{'_created'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_created'};
-
-}
-
-=head2 modified
-
- Title : modified
- Usage : $obj->modified()
- Function:
- Returns : value of modified
- Args :
-
-
-=cut
-
-sub modified{
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_modified'} = $value;
- }
-
- if( exists $self->{'_modified'} ) {
- return $self->{'_modified'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_modified'};
-}
-
-
-=head2 version
-
- Title : version
- Usage : $obj->version()
- Function:
- Returns : value of version
- Args :
-
-=cut
-
-sub version{
-
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_version'} = $value;
- }
-
- if( exists $self->{'_version'} ) {
- return $self->{'_version'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_version'};
-
-}
-
-
-=head2 stable_id
-
- Title : stable_id
- Usage : $obj->stable_id
- Function:
- Returns : value of stable_id
- Args :
-
-
-=cut
-
-sub stable_id{
-
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_stable_id'} = $value;
- }
-
- if( exists $self->{'_stable_id'} ) {
- return $self->{'_stable_id'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_stable_id'};
-
-}
-
-sub _get_stable_entry_info {
+sub version {
my $self = shift;
-
- if( !defined $self->adaptor ) {
- return undef;
- }
-
- $self->adaptor->get_stable_entry_info($self);
-
+ $self->{'version'} = shift if( @_ );
+ return $self->{'version'};
}
@@ -1102,7 +657,7 @@ sub load_genomic_mapper {
my ( $self, $mapper, $id, $start ) = @_;
$mapper->add_map_coordinates( $id, $start, $start+$self->length()-1,
- $self->strand(), $self->contig,
+ $self->strand(), $self->slice(),
$self->start(), $self->end() );
}
@@ -1167,12 +722,17 @@ sub peptide {
my $tr = shift;
unless($tr && ref($tr) && $tr->isa('Bio::EnsEMBL::Transcript')) {
- $self->throw("transcript arg must be Bio::EnsEMBL:::Transcript not [$tr]");
+ throw("transcript arg must be Bio::EnsEMBL:::Transcript not [$tr]");
}
#convert exons coordinates to peptide coordinates
+ my $tmp_exon = $self->transfer($tr->slice);
+ if (!$tmp_exon) {
+ throw("Couldn't transfer exon to transcript's slice");
+ }
+
my @coords =
- $tr->genomic2pep($self->start, $self->end, $self->strand, $self->contig);
+ $tr->genomic2pep($tmp_exon->start, $tmp_exon->end, $tmp_exon->strand);
#filter out gaps
@coords = grep {$_->isa('Bio::EnsEMBL::Mapper::Coordinate')} @coords;
@@ -1181,7 +741,7 @@ sub peptide {
my $pep_str = '';
if(scalar(@coords) > 1) {
- $self->throw("Error. Exon maps to multiple locations in peptide." .
+ throw("Error. Exon maps to multiple locations in peptide." .
" Is this exon [$self] a member of this transcript [$tr]?");
} elsif(scalar(@coords) == 1) {
my $c = $coords[0];
@@ -1192,7 +752,7 @@ sub peptide {
my ($start, $end);
$end = ($c->end > $pep->length) ? $pep->length : $c->end;
$start = ($c->start < $end) ? $c->start : $end;
- $pep_str = $pep->subseq($start, $end);
+ $pep_str = $tr->translate->subseq($start, $end);
}
return Bio::Seq->new(-seq => $pep_str,
@@ -1221,7 +781,7 @@ sub seq {
my $arg = shift;
if( defined $arg ) {
- $self->warn( "seq setting on Exon not supported currently" );
+ warning( "seq setting on Exon not supported currently" );
$self->{'_seq_cache'} = $arg->seq();
}
@@ -1231,13 +791,13 @@ sub seq {
my $seq;
- if ( ! defined $self->contig ) {
- $self->warn(" this exon doesn't have a contig you won't get a seq \n");
+ if ( ! defined $self->slice ) {
+ warning(" this exon doesn't have a slice you won't get a seq \n");
return undef;
}
else {
- $seq = $self->contig()->subseq($self->start, $self->end);
+ $seq = $self->slice()->subseq($self->start, $self->end);
if($self->strand == -1){
$seq =~ tr/ATGCatgc/TACGtacg/;
@@ -1255,169 +815,138 @@ sub seq {
-# Inherited methods
-# but you do have all the SeqFeature documentation: reproduced here
-# for convenience...
-
-=pod
-
-=head1 Methods inherited from SeqFeature
-
-=head2 start
-
- Title : start
- Usage : $start = $feat->start
- Function: Returns the start coordinate of the feature
- Returns : integer
- Args : none
-
-=head2 end
-
- Title : end
- Usage : $end = $feat->end
- Function: Returns the end coordinate of the feature
- Returns : integer
- Args : none
-
-=head2 strand
-
- Title : strand
- Usage : $strand = $feat->strand()
- $feat->strand($strand)
- Function: get/set on strand information, being 1,-1 or 0
- Returns : -1,1 or 0
- Args : none
+=head2 hashkey
+ Arg [1] : none
+ Example : if(exists $hash{$exon->hashkey}) { do_something(); }
+ Description: Returns a unique hashkey that can be used to uniquely identify
+ this exon. Exons are considered to be identical if they share
+ the same seq_region, start, end, strand, phase, end_phase.
+ Note that this will consider two exons on different slices
+ to be different, even if they actually are not.
+ Returntype : string formatted as slice_name-start-end-strand-phase-end_phase
+ Exceptions : thrown if not all the necessary attributes needed to generate
+ a unique hash value are set
+ set
+ Caller : general
=cut
-=head2 length
-
- Title : length
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
-
-=head2 sub_SeqFeature
-
- Title : sub_SeqFeature
- Usage : @feats = $feat->sub_SeqFeature();
- Function: Returns an array of sub Sequence Features
- Returns : An array
- Args : none
-
-=head2 primary_tag
-
- Title : primary_tag
- Usage : $tag = $feat->primary_tag()
- Function: Returns the primary tag for a feature,
- eg 'exon'
- Returns : a string
- Args : none
+sub hashkey {
+ my $self = shift;
-=head2 source_tag
+ my $slice = $self->{'slice'};
+ my $slice_name = ($slice) ? $slice->name() : undef;
+ my $start = $self->{'start'};
+ my $end = $self->{'end'};
+ my $strand = $self->{'strand'};
+ my $phase = $self->{'phase'};
+ my $end_phase = $self->{'end_phase'};
- Title : source_tag
- Usage : $tag = $feat->source_tag()
- Function: Returns the source tag for a feature,
- eg, 'genscan'
- Returns : a string
- Args : none
+ if(!defined($slice_name)) {
+ throw('Slice must be set to generate correct hashkey.');
+ }
-=head2 has_tag
+ if(!defined($start)) {
+ warning("start attribute must be defined to generate correct hashkey.");
+ }
- Title : has_tag
- Usage : $value = $self->has_tag('some_tag')
- Function: Returns the value of the tag (undef if
- none)
- Returns :
- Args :
+ if(!defined($end)) {
+ throw("end attribute must be defined to generate correct hashkey.");
+ }
-=head2 all_tags
+ if(!defined($strand)) {
+ throw("strand attribute must be defined to generate correct hashkey.");
+ }
- Title : all_tags
- Usage : @tags = $feat->all_tags()
- Function: gives all tags for this feature
- Returns : an array of strings
- Args : none
+ if(!defined($phase)) {
+ throw("phase attribute must be defined to generate correct hashkey.");
+ }
-=head2 gff_string
+ if(!defined($end_phase)) {
+ throw("end_phase attribute must be defined to generate correct hashkey.");
+ }
- Title : gff_string
- Usage : $str = $feat->gff_string
- Function: provides the feature information in GFF
- version 2 format.
- Returns : A string
- Args : None
+ return "$slice_name-$start-$end-$strand-$phase-$end_phase";
+}
+=head2 display_id
-=head1 RangeI methods
+ Arg [1] : none
+ Example : print $exons->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For exons this is the stable id if
+ it is available otherwise it is an empty string.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
-These methods are inherited from RangeI and can be used
-directly from a SeqFeatureI interface. Remember that a
-SeqFeature is-a RangeI, and so wherever you see RangeI you
-can use a feature ($r in the below documentation).
+=cut
-=head2 overlaps
+sub display_id {
+ my $self = shift;
+ return $self->{'stable_id'} || '';
+}
- Title : overlaps
- Usage : if($feat->overlaps($r)) { do stuff }
- if($feat->overlaps(200)) { do stuff }
- Function: tests if $feat overlaps $r
- Args : a RangeI to test for overlap with, or a point
- Returns : true if the Range overlaps with the feature, false otherwise
+#####################
+# DEPRECATED METHODS
+#####################
-=head2 contains
+sub _get_stable_entry_info {
+ my $self = shift;
+ deprecate( "This function shouldnt be called any more" );
+ if( !defined $self->adaptor ) {
+ return undef;
+ }
+ $self->adaptor->get_stable_entry_info($self);
+}
- Title : contains
- Usage : if($feat->contains($r) { do stuff }
- Function: tests whether $feat totally contains $r
- Args : a RangeI to test for being contained
- Returns : true if the argument is totaly contained within this range
+=head2 temporary_id
-=head2 equals
+ Description: DEPRECATED. This should not be necessary
- Title : equals
- Usage : if($feat->equals($r))
- Function: test whether $feat has the same start, end, strand as $r
- Args : a RangeI to test for equality
- Returns : true if they are describing the same range
+=cut
+sub temporary_id {
+ my $self = shift;
+ deprecate('It should not be necessary to use this method.');
+ $self->{'tempID'} = shift if(@_);
+ return $self->{'tempID'};
+}
-=head1 Geometrical methods
+=head2 created
-These methods do things to the geometry of ranges, and return
-triplets (start, stop, strand) from which new ranges could be built.
+ Description: DEPRECATED. Do not use.
=cut
-=head2 intersection
-
- Title : intersection
- Usage : ($start, $stop, $strand) = $feat->intersection($r)
- Function: gives the range that is contained by both ranges
- Args : a RangeI to compare this one to
- Returns : nothing if they don''t overlap, or
- a new exon based on the range that they do overlap
-
+sub created{
+ my ($self,$value) = @_;
+ deprecate( "Created attribute not supported any more" );
+ if(defined $value ) {
+ $self->{'_created'} = $value;
+ }
+ return $self->{'_created'};
+}
-=head2 union
+=head2 modified
- Title : union
- Usage : ($start, $stop, $strand) = $feat->union($r);
- : ($start, $stop, $strand) = Bio::RangeI->union(@ranges);
- Function: finds the minimal range that contains all of the ranges
- Args : a range or list of ranges to find the union of
- Returns : the range containing all of the ranges
+ Description: DEPRECATED. Do not use.
=cut
+sub modified{
+ my ($self,$value) = @_;
+ deprecate( "Modified attribute not supported any more" );
+ if( defined $value ) {
+ $self->{'_modified'} = $value;
+ }
+ return $self->{'_modified'};
+}
+
1;
diff --git a/modules/Bio/EnsEMBL/External/BlastAdaptor.pm b/modules/Bio/EnsEMBL/External/BlastAdaptor.pm
index 4e40198bbc..61ac03f23f 100644
--- a/modules/Bio/EnsEMBL/External/BlastAdaptor.pm
+++ b/modules/Bio/EnsEMBL/External/BlastAdaptor.pm
@@ -5,176 +5,16 @@ use strict;
use DBI;
use Storable qw(freeze thaw);
use Data::Dumper qw( Dumper );
-use Time::Local;
use vars qw(@ISA);
use Bio::EnsEMBL::DBSQL::BaseAdaptor;
use Bio::EnsEMBL::DBSQL::DBConnection;
-use Bio::Search::HSP::EnsemblHSP; # This is a web module
+#use Bio::Search::HSP::EnsemblHSP; # This is a web module
@ISA = qw( Bio::EnsEMBL::DBSQL::BaseAdaptor );
#@ISA = qw( Bio::EnsEMBL::DBSQL::DBConnection );
-
-#----------------------------------------------------------------------
-# Define SQL
-
-#--- CREATE TABLES ---
-our $SQL_CREATE_TICKET = "
-CREATE TABLE blast_ticket (
- ticket_id int(10) unsigned NOT NULL auto_increment,
- create_time datetime NOT NULL default '0000-00-00 00:00:00',
- update_time datetime NOT NULL default '0000-00-00 00:00:00',
- ticket varchar(32) NOT NULL default '',
- status enum('CURRENT','DELETED') NOT NULL default 'CURRENT',
- object longblob,
- PRIMARY KEY (ticket_id),
- UNIQUE KEY ticket (ticket),
- KEY create_time (create_time),
- KEY update_time (update_time)
-) TYPE=MyISAM";
-
-our $SQL_CREATE_TABLE_LOG = "
-CREATE TABLE blast_table_log (
- table_id int(10) unsigned NOT NULL auto_increment,
- table_name varchar(32),
- table_type enum('TICKET','RESULT','HIT','HSP') default NULL,
- table_status enum('CURRENT','FILLED','DELETED') default NULL,
- use_date date default NULL,
- create_time datetime default NULL,
- delete_time datetime default NULL,
- num_objects int(10) default NULL,
- PRIMARY KEY (table_id),
- KEY table_name (table_name),
- KEY table_type (table_type),
- KEY use_date (use_date),
- KEY table_status (table_status)
-) TYPE=MyISAM";
-
-
-our $SQL_CREATE_DAILY_RESULT = "
-CREATE TABLE %s (
- result_id int(10) unsigned NOT NULL auto_increment,
- ticket varchar(32) default NULL,
- object longblob,
- PRIMARY KEY (result_id),
- KEY ticket (ticket)
-) TYPE=MyISAM";
-
-our $SQL_CREATE_DAILY_HIT = "
-CREATE TABLE %s (
- hit_id int(10) unsigned NOT NULL auto_increment,
- ticket varchar(32) default NULL,
- object longblob,
- PRIMARY KEY (hit_id),
- KEY ticket (ticket)
-) TYPE=MyISAM";
-
-our $SQL_CREATE_DAILY_HSP = "
-CREATE TABLE %s (
- hsp_id int(10) unsigned NOT NULL auto_increment,
- ticket varchar(32) default NULL,
- object longblob,
- chr_name varchar(32) default NULL,
- chr_start int(10) unsigned default NULL,
- chr_end int(10) unsigned default NULL,
- PRIMARY KEY (hsp_id),
- KEY ticket (ticket)
-) TYPE=MyISAM MAX_ROWS=705032704 AVG_ROW_LENGTH=4000";
-
-#--- TABLE LOG ---
-our $SQL_SELECT_TABLE_LOG_CURRENT = "
-SELECT use_date
-FROM blast_table_log
-WHERE table_type = ?
-AND table_status = 'CURRENT'
-ORDER BY use_date DESC";
-
-our $SQL_TABLE_LOG_INSERT = "
-INSERT into blast_table_log
- ( table_name, table_status, table_type, use_date, create_time)
-VALUES ( ?, ?, ?, ?, NOW() )";
-
-our $SQL_TABLE_LOG_UPDATE = "
-UPDATE blast_table_log
-SET table_status = ?,
- delete_time = ?,
- num_objects = ?
-WHERE table_name = ?";
-
-#--- TICKETS ---
-
-our $SQL_SEARCH_MULTI_STORE = "
-INSERT INTO blast_ticket ( create_time, update_time, object, ticket )
-VALUES ( NOW(), NOW(), ? , ? )";
-
-our $SQL_SEARCH_MULTI_UPDATE = "
-UPDATE blast_ticket
-SET object = ?,
- update_time = NOW()
-WHERE ticket = ?";
-
-our $SQL_SEARCH_MULTI_RETRIEVE = "
-SELECT object
-FROM blast_ticket
-WHERE ticket = ? ";
-
-#--- RESULTS ---
-
-our $SQL_RESULT_STORE = "
-INSERT INTO blast_result%s ( object, ticket )
-VALUES ( ? , ? )";
-
-our $SQL_RESULT_UPDATE = "
-UPDATE blast_result%s
-SET object = ?,
- ticket = ?
-WHERE result_id = ?";
-
-our $SQL_RESULT_RETRIEVE = "
-SELECT object
-FROM blast_result%s
-WHERE result_id = ? ";
-
-#--- HITS ---
-
-our $SQL_HIT_STORE = "
-INSERT INTO blast_hit%s ( object, ticket )
-VALUES ( ? , ? )";
-
-our $SQL_HIT_UPDATE = "
-UPDATE blast_hit%s
-SET object = ?,
- ticket = ?
-WHERE hit_id = ?";
-
-our $SQL_HIT_RETRIEVE = "
-SELECT object
-FROM blast_hit%s
-WHERE hit_id = ? ";
-
-#--- HSPS ---
-
-our $SQL_HSP_STORE = "
-INSERT INTO blast_hsp%s ( object, ticket, chr_name, chr_start, chr_end )
-VALUES ( ? , ? , ? , ? , ? )";
-
-our $SQL_HSP_UPDATE = "
-UPDATE blast_hsp%s
-SET object = ?,
- ticket = ?,
- chr_name = ?,
- chr_start = ?,
- chr_end = ?
-WHERE hsp_id = ?";
-
-our $SQL_HSP_RETRIEVE = "
-SELECT object
-FROM blast_hsp%s
-WHERE hsp_id = ? ";
-
-
#----------------------------------------------------------------------
=head2 new
@@ -195,413 +35,6 @@ sub new {
return $self;
}
-#----------------------------------------------------------------------
-
-=head2 store
-
- Arg [1] :
- Function :
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub store {
- my $self = shift;
- my $obj = shift;
- my $ret;
- if( $obj->isa("Bio::Tools::Run::SearchMulti") ){
- $ret = $self->store_search_multi( $obj, @_ );
- }
- elsif( $obj->isa("Bio::Search::Result::ResultI") ){
- $ret = $self->store_result( $obj, @_ );
- }
- elsif( $obj->isa("Bio::Search::Hit::HitI") ){
- $ret =$self->store_hit( $obj, @_ );
- }
- elsif( $obj->isa("Bio::Search::HSP::HSPI") ){
- $ret = $self->store_hsp( $obj, @_ );
- }
- else{
- $self->throw( "Do not know how to store objects of type ".ref($obj) );
- }
- return $ret;
-}
-
-#----------------------------------------------------------------------
-
-=head2 retrieve
-
- Arg [1] :
- Function :
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub retrieve {
- my $self = shift;
- my $caller = shift;
- if( $caller->isa("Bio::Tools::Run::EnsemblSearchMulti") ){
- return $self->retrieve_search_multi( @_ );
- }
- elsif( $caller->isa("Bio::Search::Result::ResultI") ){
- return $self->retrieve_result( @_ );
- }
- elsif( $caller->isa("Bio::Search::Hit::HitI") ){
- return $self->retrieve_hit( @_ );
- }
- elsif( $caller->isa("Bio::Search::HSP::HSPI") ){
- return $self->retrieve_hsp( @_ );
- }
- $self->throw( "Do not know how to retrieve objects of type ".
- ref($caller)? ref($caller) : $caller );
-}
-
-#----------------------------------------------------------------------
-
-=head2 remove
-
- Arg [1] :
- Function : TODO: implement remove functions
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub remove {
- my $self = shift;
- my $obj = shift;
- if( $obj->isa("Bio::Tools::Run::EnsemblSearchMulti") ){
- #return $self->remove_search_multi( @_ );
- }
- elsif( $obj->isa("Bio::Search::Result::ResultI") ){
- #return $self->remove_result( @_ );
- }
- elsif( $obj->isa("Bio::Search::Hit::HitI") ){
- #return $self->remove_hit( @_ );
- }
- elsif( $obj->isa("Bio::Search::HSP::HSPI") ){
- #return $self->remove_hsp( @_ );
- }
- $self->throw( "Do not know how to remove objects of type ".
- ref($obj) );
-}
-#----------------------------------------------------------------------
-=head2 store_search_multi
-
- Arg [1] : Bio::Tools::Run::EnsemblSearchMulti obj
- Function : Stores the ensembl SearchMulti container object in the database
- Returntype: scalar (token)
- Exceptions:
- Caller :
- Example : my $container_token = $blast_adpt->store_ticket( $container );
-
-=cut
-
-sub store_search_multi{
- my $self = shift;
- my $search_multi = shift ||
- $self->throw( "Need a Bio::Tools::Run::EnsemblSearchMulti obj" );
-
- my $frozen = shift || $search_multi->serialise;
-
- my $dbh = $self->db->db_handle;
-
- my $ticket = $search_multi->token ||
- $self->throw( "Bio::Tools::Run::EnsemblSearchMulti obj has no ticket" );
-
- my $sth = $dbh->prepare( $SQL_SEARCH_MULTI_RETRIEVE );
- my $rv = $sth->execute( $ticket ) || $self->throw( $sth->errstr );
- $sth->finish;
-
- if( $rv < 1 ){ # Insert (do first to minimise risk of race)
- my $sth = $dbh->prepare( $SQL_SEARCH_MULTI_STORE );
- $sth->execute( $frozen, $ticket ) || $self->throw( $sth->errstr );
- #$search_multi->token( $self->dbh->{mysql_insertid} );
- $sth->finish;
- }
- else{ # Update
- my $sth = $dbh->prepare( $SQL_SEARCH_MULTI_UPDATE );
- $sth->execute( $frozen, $ticket ) || $self->throw( $sth->errstr );
- $sth->finish;
- }
- return $search_multi->token();
-}
-
-#----------------------------------------------------------------------
-
-=head2 retrieve_search_multi
-
- Arg [1] :
- Function :
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub retrieve_search_multi {
- my $self = shift;
- my $ticket = shift || $self->throw( "Need an EnsemblSearchMulti ticket" );
-
- my $dbh = $self->db->db_handle;
- my $sth = $dbh->prepare( $SQL_SEARCH_MULTI_RETRIEVE );
- my $rv = $sth->execute( $ticket ) || $self->throw( $sth->errstr );
- if( $rv < 1 ){ $self->throw( "Token $ticket not found" ) }
- my ( $frozen ) = $sth->fetchrow_array;
- $frozen || $self->throw( "Object from ticket $ticket is empty" );
- $sth->finish;
- return $frozen;
-}
-
-
-
-#----------------------------------------------------------------------
-=head2 store_result
-
- Arg [1] : Bio::Search::Result::EnsemblResult obj
- Function : Stores the ensembl Result in the database
- Returntype: scalar (token)
- Exceptions:
- Caller :
- Example : my $result_token = $blast_adpt->store_result( $result );
-
-=cut
-
-sub store_result{
- my $self = shift;
- my $res = shift ||
- $self->throw( "Need a Bio::Search::Result::EnsemblResult obj" );
- my $frozen = shift || $res->serialised;
-
- my $dbh = $self->db->db_handle;
-
- my $ticket = $res->group_ticket;
- my ( $id, $use_date ) = split( '!!', $res->token || '' );
- $use_date ||= $self->use_date('RESULT');
-
- my $rv = 0;
- if( $id ){
- my $sth = $dbh->prepare( sprintf $SQL_RESULT_RETRIEVE, $use_date );
- $rv = $sth->execute( $id ) || $self->throw( $sth->errstr );
- $sth->finish;
- }
- if( $rv < 1 ){# Insert
- my $use_date = $res->use_date() ||
- $res->use_date($self->use_date('RESULT'));
- my $sth = $dbh->prepare( sprintf $SQL_RESULT_STORE, $use_date );
- $sth->execute( $frozen, $ticket ) || $self->throw( $sth->errstr );
- my $id = $dbh->{mysql_insertid};
- $res->token( join( '!!', $id, $use_date ) );
- $sth->finish;
- }
- else{ # Update
- my $sth = $dbh->prepare( sprintf $SQL_RESULT_UPDATE, $use_date );
- $sth->execute( $frozen, $ticket, $id ) || $self->throw( $sth->errstr );
- $sth->finish;
- }
- return $res->token();
-}
-
-#----------------------------------------------------------------------
-
-=head2 retrieve_result
-
- Arg [1] :
- Function :
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub retrieve_result{
- my $self = shift;
- my $token = shift || $self->throw( "Need a Hit token" );
- my ( $id, $use_date ) = split( '!!',$token);
- $use_date ||= '';
-
- my $dbh = $self->db->db_handle;
- my $sth = $dbh->prepare( sprintf $SQL_RESULT_RETRIEVE, $use_date );
- my $rv = $sth->execute( $id ) || $self->throw( $sth->errstr );
- if( $rv < 1 ){ $self->throw( "Token $id not found" ) }
- my ( $frozen ) = $sth->fetchrow_array;
- $frozen || $self->throw( "Object from result $id is empty" );
- $sth->finish;
- return $frozen;
-}
-
-#----------------------------------------------------------------------
-=head2 store_hit
-
- Arg [1] : Bio::Search::Hit::EnsemblHit obj
- Function : Stores the ensembl Hit in the database
- Returntype: scalar (token)
- Exceptions:
- Caller :
- Example : my $hit_token = $blast_adpt->store_hit( $hit );
-
-=cut
-
-sub store_hit{
- my $self = shift;
- my $hit = shift ||
- $self->throw( "Need a Bio::Search::Hit::EnsemblHit obj" );
- my $frozen = shift || $hit->serialise;
-
- my $dbh = $self->db->db_handle;
-
- my $ticket = $hit->group_ticket;
- my ( $id, $use_date ) = split( '!!', $hit->token || '' );
- $use_date ||= '';
-
- my $rv = 0;
- if( $id ){
- my $sth = $dbh->prepare( sprintf $SQL_HIT_RETRIEVE, $use_date );
- $rv = $sth->execute( $id ) || $self->throw( $sth->errstr );
- $sth->finish;
- }
- if( $rv < 1 ){ # Insert
- my $use_date = $hit->use_date() || $hit->use_date($self->use_date('HIT'));
- my $sth = $dbh->prepare( sprintf $SQL_HIT_STORE, $use_date );
- $sth->execute( $frozen, $ticket ) || $self->throw( $sth->errstr );
- my $id = $dbh->{mysql_insertid};
- $hit->token( join( '!!', $id, $use_date ) );
- $sth->finish;
- }
- else{ # Update
- my $sth = $dbh->prepare( sprintf $SQL_HIT_UPDATE, $use_date );
- $sth->execute( $frozen, $ticket, $id ) || $self->throw( $sth->errstr );
- $sth->finish;
- }
- return $hit->token();
-}
-
-#----------------------------------------------------------------------
-
-=head2 retrieve_hit
-
- Arg [1] :
- Function :
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub retrieve_hit{
- my $self = shift;
- my $token = shift || $self->throw( "Need a Hit token" );
- my ( $id, $use_date ) = split( '!!',$token);
- $use_date ||= '';
-
- my $dbh = $self->db->db_handle;
- my $sth = $dbh->prepare( sprintf $SQL_HIT_RETRIEVE, $use_date );
- my $rv = $sth->execute( $id ) || $self->throw( $sth->errstr );
- if( $rv < 1 ){ $self->throw( "Token $token not found" ) }
- my ( $frozen ) = $sth->fetchrow_array;
- $frozen || $self->throw( "Object from hit $id is empty" );
- $sth->finish;
- return $frozen;
-}
-
-#----------------------------------------------------------------------
-=head2 store_hsp
-
- Arg [1] : Bio::Search::HSP::EnsemblHSP obj
- Function : Stores the ensembl HSP in the database
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub store_hsp{
- my $self = shift;
- my $hsp = shift ||
- $self->throw( "Need a Bio::Search::HSP::EnsemblHSP obj" );
- my $frozen = shift || $hsp->serialise;
-
- my $dbh = $self->db->db_handle;
-
- my $ticket = $hsp->group_ticket;
- my ( $id, $use_date ) = split( '!!', $hsp->token || '');
- $use_date ||= $self->use_date('HSP');
-
- my $chr_name = 'NULL';
- my $chr_start = 'NULL';
- my $chr_end = 'NULL';
- if( my $genomic = $hsp->genomic_hit ){
- $chr_name = $genomic->seq_id;
- $chr_start = $genomic->start;
- $chr_end = $genomic->end;
- }
-
- my $rv = 0;
- if( $id ){
- my $sth = $dbh->prepare( sprintf $SQL_HSP_RETRIEVE, $use_date );
- $rv = $sth->execute( $id ) || $self->throw( $sth->errstr );
- $sth->finish;
- }
- if( $rv < 1 ){ # Insert
- my $use_date = $hsp->use_date() || $hsp->use_date($self->use_date('HSP'));
- my $sth = $dbh->prepare( sprintf $SQL_HSP_STORE, $use_date );
- my @bound = ( $frozen, $ticket, $chr_name, $chr_start, $chr_end );
- $sth->execute( @bound ) || $self->throw( $sth->errstr );
- my $id = $dbh->{mysql_insertid};
- $hsp->token( join( '!!', $id, $use_date ) );
- $sth->finish;
- }
- else{ # Update
- my $sth = $dbh->prepare( sprintf $SQL_HSP_UPDATE, $use_date );
- my @bound = ( $frozen, $ticket, $chr_name, $chr_start, $chr_end, $id );
- $sth->execute( @bound ) || $self->throw( $sth->errstr );
- $sth->finish;
- }
- return $hsp->token();
-}
-
-#----------------------------------------------------------------------
-
-=head2 retrieve_hsp
-
- Arg [1] :
- Function :
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub retrieve_hsp{
- my $self = shift;
- my $token = shift || $self->throw( "Need an HSP token" );
- my ( $id, $use_date ) = split( '!!',$token);
- $use_date ||= '';
-
- my $dbh = $self->db->db_handle;
- my $sth = $dbh->prepare( sprintf $SQL_HSP_RETRIEVE, $use_date );
- my $rv = $sth->execute( $id ) || $self->throw( $sth->errstr );
- if( $rv < 1 ){ $self->throw( "Token $token not found" ) }
- my ( $frozen ) = $sth->fetchrow_array;
- $frozen || $self->throw( "Object from hsp $id is empty" );
- $sth->finish;
- return $frozen;
-}
-
-
#----------------------------------------------------------------------
=head2 get_all_HSPs
@@ -621,13 +54,11 @@ sub get_all_HSPs {
my $chr_name = shift || undef;
my $chr_start = shift || undef;
my $chr_end = shift || undef;
- my ( $id, $use_date ) = split( '!!', $ticket );
- $use_date ||= '';
my $SQL = qq(
SELECT object
-FROM blast_hsp%s
-WHERE \(ticket = ? OR ticket = ?\) );
+FROM blast_hsp
+WHERE ticket = ? );
my $CHR_SQL = qq(
AND chr_name = ? );
@@ -636,8 +67,8 @@ AND chr_name = ? );
AND chr_start <= ?
AND chr_end >= ? );
- my $q = sprintf( $SQL, $use_date );
- my @binded = ( $id, substr($id,6) );
+ my $q = $SQL;
+ my @binded = ( $ticket );
if( $chr_name ){
$q .= $CHR_SQL;
@@ -648,13 +79,12 @@ AND chr_end >= ? );
push @binded, $chr_end, $chr_start;
}
}
-# warn( "$q: ", join( ', ',@binded ) );
+ warn( "$q: ", join( ', ',@binded ) );
my $sth = $self->db->db_handle->prepare($q);
my $rv = $sth->execute( @binded ) || $self->throw( $sth->errstr );
my @hsps = map{ thaw( $_->[0] ) } @{$sth->fetchall_arrayref()};
- $sth->finish;
return [@hsps];
}
@@ -677,301 +107,12 @@ AND chr_end >= ? );
sub get_all_SearchFeatures {
my $self = shift;
my $hsps = $self->get_all_HSPs(@_);
- $self->dynamic_use( ref($hsps->[0] ) );
my @feats = grep{ $_ } map{ $_->ens_genomic_align } @$hsps;
return [ @feats ];
}
-sub dynamic_use {
- my( $self, $classname ) = @_;
- my( $parent_namespace, $module ) = $classname =~/^(.*::)(.*?)$/;
- no strict 'refs';
- return 1 if $parent_namespace->{$module.'::'}; # return if already used
- eval "require $classname";
- if($@) {
- warn "DrawableContainer: failed to use $classname\nDrawableContainer: $@";
- return 0;
- }
- $classname->import();
- return 1;
-}
-
-#----------------------------------------------------------------------
-
-=head2 use_date
-
- Arg [1] :
- Function :
- Returntype:
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-my %valid_table_types = ( HIT=>1, HSP=>1, RESULT=>1 );
-sub use_date {
- my $key = '_current_table';
- my $self = shift;
- my $type = uc( shift );
- $valid_table_types{$type} ||
- $self->throw( "Need a table type (Result, Hit or HSP)" );
-
- $self->{$key} ||= {};
- if( ! $self->{$key}->{$type} ){
- my $sth = $self->db->db_handle->prepare( $SQL_SELECT_TABLE_LOG_CURRENT );
- my $rv = $sth->execute( $type ) || ( warn( $sth->errstr ) && return );
- $rv > 0 || ( warn( "No current $type table found" ) && return );
- my $date = $sth->fetchrow_arrayref->[0];
- $date =~ s/-//g;
- $self->{$key}->{$type} = $date;
- }
- return $self->{$key}->{$type};
-}
-
-
-
-#----------------------------------------------------------------------
-
-=head2 clean_blast_database
-
- Arg [1] : int $days
- Function : Removes blast tickets older than $days days
- Returntype:
- Exceptions: SQL errors
- Caller :
- Example : $ba->clean_blast_database(14)
-
-=cut
-
-sub clean_blast_database{
- my $self = shift;
- my $days = shift || $self->throw( "Missing arg: number of days" );
- $days =~ /\D/ && $self->throw( "Bad arg: number of days $days not int" );
- my $dbh = $self->db->db_handle;
-
- # Get list of tickets > $days days old
- my $q = qq/
-SELECT ticket
-FROM blast_ticket
-WHERE update_time < SUBDATE( NOW(), INTERVAL $days DAY ) /;
-
- my $sth = $self->db->db_handle->prepare($q);
- my $rv = $sth->execute() || $self->throw( $sth->errstr );
- my $res = $sth->fetchall_arrayref;
- $sth->finish;
-
- # Delete result and ticket rows associated with old tickets
-# my $q_del_tmpl = qq/
-#DELETE
-#FROM blast_%s
-#WHERE ticket like "%s" /;
-#
-# my @types = ( 'result','ticket' );
-# my %num_deleted = map{ $_=>0 } @types;
-#
-# foreach my $row( @$res ){
-# my $ticket = $row->[0];
-#
-# foreach my $type( @types ){
-# my $q_del = sprintf( $q_del_tmpl, $type, $ticket );
-# my $sth = $self->db->db_handle->prepare($q_del);
-# my $rv = $sth->execute() || $self->throw( $sth->errstr );
-# $num_deleted{$type} += $rv;
-# }
-# }
-# map{ warn("Purging $days days: Deleted $num_deleted{$_} rows of type $_\n") }
-# keys %num_deleted;
-
- # Drop daily Result, Hit and HSP tables not updated within $days days
- my $q_find = 'show table status like ?';
- my $sth2 = $dbh->prepare( $q_find );
- $sth2->execute( "blast_result%" ) || $self->throw( $sth2->errstr );
- my $res_res = $sth2->fetchall_arrayref();
- $sth2->execute( "blast_hit%" ) || $self->throw( $sth2->errstr );
- my $hit_res = $sth2->fetchall_arrayref();
- $sth2->execute( "blast_hsp%" ) || $self->throw( $sth2->errstr );
- my $hsp_res = $sth2->fetchall_arrayref();
-
- my @deletable_hit_tables;
- foreach my $row( @$res_res, @$hit_res, @$hsp_res ){
- my $table_name = $row->[0];
- my $num_rows = $row->[3];
- my $update_time = $row->[11]; # Should be a string like 2003-08-15 10:36:56
- my @time = split( /[-:\s]/, $update_time );
-
- my $epoch_then = timelocal( $time[5], $time[4], $time[3],
- $time[2], $time[1]-1, $time[0] - 1900 );
- my $secs_old = time() - $epoch_then;
- my $days_old = $secs_old / ( 60 * 60 * 24 );
- if( $days_old > $days ){
- warn( "Dropping table $table_name: $num_rows rows\n" );
- my $sth_drop = $dbh->prepare( "DROP table $table_name" );
- my $sth_log = $dbh->prepare( $SQL_TABLE_LOG_UPDATE );
- $sth_drop->execute || $self->throw( $sth_drop->errstr );
- my( $se,$mi,$hr,$da,$mo,$yr ) = (localtime)[0,1,2,3,4,5];
- my $now = sprintf( "%4d-%2d-%2d %2d:%2d:%2d",
- $yr+1900,$mo+1,$da,$hr,$mi,$se );
- $sth_log->execute
- ('DELETED',$now,$num_rows,$table_name) ||
- $self->throw( $sth_log->errstr );
- }
- }
-
-
- return 1;
-}
-
-#----------------------------------------------------------------------
-
-=head2 create_tables
- Arg [1] : none
- Function : Creates the blast_ticket and blast_table_log
- tables in the database indicated by the database handle.
- Checks first to make sure they do not exist
- Returntype: boolean
- Exceptions:
- Caller :
- Example :
-=cut
-
-sub create_tables {
- my $self = shift;
- my $dbh = $self->db->db_handle;
-
- # Get list of existing tables in database
- my $q = 'show tables like ?';
- my $sth = $dbh->prepare( $q );
- my $rv_tck = $sth->execute("blast_ticket") || $self->throw($sth->errstr);
- my $rv_log = $sth->execute("blast_table_log" )|| $self->throw($sth->errstr);
- $sth->finish;
-
- if( $rv_tck == 0 ){
- warn( "Creating blast_ticket table\n" );
- my $sth = $dbh->prepare( $SQL_CREATE_TICKET );
- my $rv = $sth->execute() || $self->throw( $sth->errstr );
- $sth->finish;
- }
- else{ warn( "blast_ticket table already exists\n" ) }
-
- if( $rv_log == 0 ){
- warn( "Creating blast_result table\n" );
- my $sth = $dbh->prepare( $SQL_CREATE_TABLE_LOG );
- my $rv = $sth->execute() || $self->throw( $sth->errstr );
- $sth->finish;
- }
- else{ warn( "blast_table_log table already exists\n" ) }
-
- return 1;
-}
-
-#----------------------------------------------------------------------
-
-=head2 rotate_daily_tables
-
- Arg [1] : none
- Function : Creates the daily blast_result{date}, blast_hit{date}
- and blast_hsp{date} tables in the database indicated by
- the database handle.
- Checks first to make sure they do not exist.
- Sets the new table to 'CURRENT' in the blast_table_log.
- Sets the previous 'CURRENT' table to filled.
- Returntype: boolean
- Exceptions:
- Caller :
- Example :
-
-=cut
-
-sub rotate_daily_tables {
- my $self = shift;
- my $dbh = $self->db->db_handle;
-
- # Get date
- my( $day, $month, $year ) = (localtime)[3,4,5];
- my $date = sprintf( "%04d%02d%02d", $year+1900, $month+1, $day );
-
- my $res_table = "blast_result$date";
- my $hit_table = "blast_hit$date";
- my $hsp_table = "blast_hsp$date";
-
- # Get list of existing tables in database
- my $q = 'show table status like ?';
- my $sth = $dbh->prepare( $q );
- my $rv_res = $sth->execute($res_table) || $self->throw($sth->errstr);
- my $rv_hit = $sth->execute($hit_table) || $self->throw($sth->errstr);
- my $rv_hsp = $sth->execute($hsp_table) || $self->throw($sth->errstr);
- $sth->finish;
-
- if( $rv_res == 0 ){
- warn( "Creating today's $res_table table\n" );
-
- # Create new table
- my $q = sprintf($SQL_CREATE_DAILY_RESULT, $res_table);
- my $sth1 = $dbh->prepare( $q );
- my $rv = $sth1->execute() || $self->throw( $sth1->errstr );
-
- # Flip current table in blast_table_tog
- my $last_date = $self->use_date( "RESULT" ) || '';
- my $sth2 = $dbh->prepare( $SQL_TABLE_LOG_INSERT );
- my $sth3 = $dbh->prepare( $SQL_TABLE_LOG_UPDATE );
- $sth2->execute( "$res_table",'CURRENT','RESULT',$date )
- || die( $self->throw( $sth2->errstr ) );
- $sth3->execute( 'FILLED','0',0,"blast_result$last_date")
- || die( $self->throw( $sth3->errstr ) );
- $sth1->finish();
- $sth2->finish();
- $sth3->finish();
- }
- else{ warn( "Today's $res_table table already exists\n" ) }
-
- if( $rv_hit == 0 ){
- warn( "Creating today's $hit_table table\n" );
-
- # Create new table
- my $q = sprintf($SQL_CREATE_DAILY_HIT, $hit_table);
- my $sth1 = $dbh->prepare( $q );
- my $rv = $sth1->execute() || $self->throw( $sth1->errstr );
-
- # Flip current table in blast_table_tog
- my $last_date = $self->use_date( "HIT" ) || '';
- my $sth2 = $dbh->prepare( $SQL_TABLE_LOG_INSERT );
- my $sth3 = $dbh->prepare( $SQL_TABLE_LOG_UPDATE );
- $sth2->execute( "$hit_table",'CURRENT','HIT',$date )
- || die( $self->throw( $sth2->errstr ) );
- $sth3->execute( 'FILLED','0',0,"blast_hit$last_date")
- || die( $self->throw( $sth3->errstr ) );
- $sth1->finish();
- $sth2->finish();
- $sth3->finish();
- }
- else{ warn( "Today's $hit_table table already exists\n" ) }
-
- if( $rv_hsp == 0 ){
- warn( "Creating today's $hsp_table table\n" );
-
- # Create new table
- my $q = sprintf($SQL_CREATE_DAILY_HSP, $hsp_table );
- my $sth1 = $dbh->prepare( $q );
- my $rv = $sth1->execute() || $self->throw( $sth1->errstr );
-
- # Flip current table in blast_table_tog
- my $last_date = $self->use_date( "HSP" ) || '';
- my $sth2 = $dbh->prepare( $SQL_TABLE_LOG_INSERT );
- my $sth3 = $dbh->prepare( $SQL_TABLE_LOG_UPDATE );
- $sth2->execute( "$hsp_table",'CURRENT','HSP',$date )
- || die( $self->throw( $sth2->errstr ) );
- $sth3->execute( 'FILLED','0',0,"blast_hsp$last_date")
- || die( $self->throw( $sth3->errstr ) );
- $sth1->finish();
- $sth2->finish();
- $sth3->finish();
- }
- else{ warn( "Today's $hsp_table table already exists\n" ) }
- return 1;
-}
#----------------------------------------------------------------------
1;
diff --git a/modules/Bio/EnsEMBL/External/ExternalFeatureAdaptor.pm b/modules/Bio/EnsEMBL/External/ExternalFeatureAdaptor.pm
index 2a56c187a3..312d475f93 100644
--- a/modules/Bio/EnsEMBL/External/ExternalFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/External/ExternalFeatureAdaptor.pm
@@ -7,7 +7,14 @@
=head1 NAME
-Bio::EnsEMBL::External::ExternalFeatureAdaptor - Allows features created externally from EnsEMBL in a single coordinate system to be retrieved in several other (EnsEMBL-style) coordinate systems. This is intended to be a replacement for the old Bio::EnsEMBL::DB::ExternalFeatureFactoryI interface.
+Bio::EnsEMBL::External::ExternalFeatureAdaptor
+
+=head 1 SUMMARY
+
+Allows features created externally from EnsEMBL in a single coordinate system
+to be retrieved in several other (EnsEMBL-style) coordinate systems. This is
+intended to be a replacement for the old
+Bio::EnsEMBL::DB::ExternalFeatureFactoryI interface.
=head1 SYNOPSIS
@@ -24,33 +31,22 @@ Bio::EnsEMBL::External::ExternalFeatureAdaptor - Allows features created externa
#get some features in RawContig coords
@feats = @{$xf_adaptor->fetch_all_by_contig_name('AC000087.2.1.42071')};
- #another way of doing the same thing
- $contig_adaptor = $database_adaptor->get_RawContigAdaptor;
- $contig = $contig_adaptor->fetch_by_name('AC000087.2.1.42071');
- @feats = @{$xf_adaptor->fetch_all_by_RawContig($contig);
-
#get some features in assembly coords
@feats = @{$xf_adaptor->fetch_all_by_chr_start_end('X', 100000, 200000)};
#get some features in clone coords
@feats = @{$xf_adaptor->fetch_all_by_clone_accession('AC000087')};
- #another way of doing the same thing
- $clone_adaptor = $db_adaptor->get_CloneAdaptor;
- $clone = $clone_adaptor->fetch_by_accession('AC000087');
- @feats = ${$xf_adaptor->fetch_all_by_Clone($clone);
-
#Add the adaptor to the ensembl core dbadaptor (implicitly sets db attribute)
$database_adaptor->add_ExternalFeatureAdaptor($xf_adaptor);
#get some features in Slice coords
$slice_adaptor = $database_adaptor->get_SliceAdaptor;
- $slice = $slice_adaptor->fetch_by_chr_start_end(1,100000,200000);
+ $slice = $slice_adaptor->fetch_all_by_chr_start_end(1,100000,200000);
@feats = @{$xf_adaptor->fetch_all_by_Slice($slice)};
- #now features can be retrieved directly from slice or RawContig
+ #now features can be retrieved directly from Slice
@feats = @{$slice->get_all_ExternalFeatures};
- @feats = @{$contig->get_all_ExternalFeatures};
=head1 DESCRIPTION
@@ -76,18 +72,11 @@ corresponding fetch methods that must be implemented:
'ASSEMBLY' fetch_all_by_chr_start_end
'CLONE' fetch_all_by_clone_accession
'CONTIG' fetch_all_by_contig_name
+ 'SUPERCONTIG' fetch_all_by_supercontig_name
'SLICE' fetch_all_by_Slice
-For convenience fetch_all_by_RawContig and fetch_all_by_Clone methods are
-also available. These methods may be overridden if desired but due to
-internal dependencies the fetch_all_by_contig_name and
-fetch_all_by_clone_accession must also be overridden if these methods are
-altered. See the method descriptions for more detail.
-
-The objects returned by the fetch methods should be Bio::SeqFeature object,
-though only the start, end, strand and attach_seq methods are actually used
-by the ExternalFeatureAdaptor. The objects which are returned by the
-ExternalFeature adaptor will be altered by the functions called.
+The objects returned by the fetch methods should be EnsEMBL or BioPerl style
+Feature objects. These objects MUST have start, end and strand methods.
Before the non-overridden ExternalFeatureAdaptor fetch methods may be called
an EnsEMBL core database adaptor must be attached to the ExternalFeatureAdaptor
@@ -95,7 +84,7 @@ an EnsEMBL core database adaptor must be attached to the ExternalFeatureAdaptor
coordinate system. This may be done implicitly by adding the
ExternalFeatureAdaptor to the database adaptor through a call to the
DBAdaptor add_ExternalFeatureAdaptor method or explicitly by calling the
-ExternalFeatureAdaptor db method.
+ExternalFeatureAdaptor ensembl_db method.
=head1 CONTACT
@@ -117,12 +106,7 @@ use strict;
package Bio::EnsEMBL::External::ExternalFeatureAdaptor;
-use vars qw(@ISA);
-
-use Bio::EnsEMBL::Root;
-
-
-@ISA = qw(Bio::EnsEMBL::Root);
+use Bio::EnsEMBL::Utils::Exception qw(warning throw);
=head2 new
@@ -195,8 +179,8 @@ sub ensembl_db {
sub coordinate_systems {
my $self = shift;
-
- $self->throw("abstract method coordinate_systems not implemented\n");
+
+ throw("abstract method coordinate_systems not implemented\n");
return '';
}
@@ -276,54 +260,181 @@ sub fetch_all_by_Slice {
my ($self, $slice) = @_;
unless($slice && ref $slice && $slice->isa('Bio::EnsEMBL::Slice')) {
- $self->throw("[$slice] is not a Bio::EnsEMBL::Slice");
+ throw("[$slice] is not a Bio::EnsEMBL::Slice");
}
my $out = [];
- my $slice_start = $slice->chr_start;
- my $slice_end = $slice->chr_end;
+ my $csa = $self->ensembl_db->get_CoordSystemAdaptor();
+
+ my $slice_start = $slice->start;
+ my $slice_end = $slice->end;
my $slice_strand = $slice->strand;
- my $slice_chr = $slice->chr_name;
+ my $slice_seq_region = $slice->seq_region_name;
+ my $coord_system = $slice->coord_system;
if($self->_supported('SLICE')) {
- $self->throw("ExternalFeatureAdaptor supports SLICE coordinate system" .
+ throw("ExternalFeatureAdaptor supports SLICE coordinate system" .
" but fetch_all_by_Slice not implemented");
- }
-
- #fetch the features in assembly coords
- $out = $self->fetch_all_by_chr_start_end($slice_chr,$slice_start,$slice_end);
-
- #convert from assembly coords to slice coords
- my($f_start, $f_end, $f_strand);
- foreach my $f (@$out) {
- if($slice_strand == 1) {
- $f_start = $f->start - $slice_start + 1;
- $f_end = $f->end - $slice_start + 1;
- $f_strand = $f->strand;
- } else {
- $f_start = $slice_end - $f->end + 1;
- $f_end = $slice_end - $f->start + 1;
- $f_strand = $f->strand * -1;
+ }
+
+ my %features;
+ my $from_coord_system;
+
+ my $fetch_method;
+
+ #
+ # Get all of the features from whatever coord system they are computed in
+ #
+ if($self->_supported('CLONE')) {
+ $fetch_method = sub {
+ my $self = shift;
+ my $name = shift;
+ my ($acc, $ver) = split(/\./, $name);
+ $self->fetch_all_by_clone_accession($acc,$ver,@_);
+ };
+ $from_coord_system = $csa->fetch_by_name('clone');
+ } elsif($self->_supported('ASSEMBLY')) {
+ $from_coord_system = $csa->fetch_by_name('toplevel');
+ $fetch_method = $self->can('fetch_all_by_chr_start_end');
+ } elsif($self->_supported('CONTIG')) {
+ $from_coord_system = $csa->fetch_by_name('contig');
+ $fetch_method = $self->can('fetch_all_by_contig_name');
+ } elsif($self->_supported('SUPERCONTIG')) {
+ $from_coord_system = $csa->fetch_by_name('supercontig');
+ $fetch_method = $self->can('fetch_all_by_supercontig_name');
+ } else {
+ $self->_no_valid_coord_systems();
+ }
+
+ if($from_coord_system->equals($coord_system)) {
+ $features{$slice_seq_region} = &$fetch_method($self, $slice_seq_region,
+ $slice_start,$slice_end);
+ } else {
+ foreach my $segment (@{$slice->project($from_coord_system->name,
+ $from_coord_system->version)}) {
+ my ($start,$end,$pslice) = @$segment;
+ $features{$pslice->seq_region_name} ||= [];
+ push @{$features{$pslice->seq_region_name}},
+ @{&$fetch_method($self, $pslice->seq_region_name,
+ $pslice->start(),
+ $pslice->end())};
+ }
+ }
+
+ my @out;
+
+ if(!$coord_system->equals($from_coord_system)) {
+ my $asma = $self->ensembl_db->get_AssemblyMapperAdaptor();
+ my $mapper = $asma->fetch_by_CoordSystems($from_coord_system,
+ $coord_system);
+ my %slice_cache;
+ my $slice_adaptor = $self->ensembl_db->get_SliceAdaptor();
+ my $slice_setter;
+
+ #convert the coordinates of each of the features retrieved
+ foreach my $fseq_region (keys %features) {
+ my $feats = $features{$fseq_region};
+ next if(!$feats);
+ $slice_setter = _guess_slice_setter($feats) if(!$slice_setter);
+
+ foreach my $f (@$feats) {
+ my($sr_name, $start, $end, $strand) =
+ $mapper->fastmap($fseq_region,$f->start,$f->end,$f->strand,
+ $from_coord_system);
+
+ #maps to gap
+ next if(!defined($sr_name));
+
+ #maps to unexpected seq region, probably error in the externally
+ if($sr_name ne $slice_seq_region) {
+ warning("Externally created Feature mapped to [$sr_name] " .
+ "which is not on requested seq_region [$slice_seq_region]");
+ next;
+ }
+
+ #update the coordinates of the feature
+ &$slice_setter($f,$slice);
+ $f->start($start);
+ $f->end($end);
+ $f->strand($strand);
+ push @out, $f;
+ }
+ }
+ } else {
+ #we already know the seqregion the featues are on, we just have
+ #to put them on the slice
+ @out = @{$features{$slice_seq_region}};
+ my $slice_setter = _guess_slice_setter(\@out);
+
+ foreach my $f (@out) {
+ &$slice_setter($f,$slice);
}
+ }
+
+ #shift the feature coordinates again if
+ #the requested slice is not the full seqregion
+ if($slice->start != 1 || $slice->strand != 1) {
+ #convert from assembly coords to slice coords
+ my($f_start, $f_end, $f_strand);
+ foreach my $f (@out) {
+ if($slice_strand == 1) {
+ $f_start = $f->start - $slice_start + 1;
+ $f_end = $f->end - $slice_start + 1;
+ $f_strand = $f->strand;
+ } else {
+ $f_start = $slice_end - $f->end + 1;
+ $f_end = $slice_end - $f->start + 1;
+ $f_strand = $f->strand * -1;
+ }
- $f->start($f_start);
- $f->end($f_end);
- $f->strand($f_strand);
- $f->attach_seq($slice);
+ $f->start($f_start);
+ $f->end($f_end);
+ $f->strand($f_strand);
+ }
}
- return $out;
+ return \@out;
}
+sub _guess_slice_setter {
+ my $features = shift;
+
+ #we do not know what type of features these are. They might
+ #be bioperl features or old ensembl features, hopefully they are new
+ #style features. Try to come up with a setter method for the
+ #slice.
+
+ return undef if(!@$features);
+
+ my ($f) = @$features;
+
+ my $slice_setter;
+ foreach my $method (qw(slice contig attach_seq)) {
+ last if($slice_setter = $f->can($method));
+ }
+
+ if(!$slice_setter) {
+ if($f->can('seqname')) {
+ $slice_setter = sub { $_[0]->seqname($_[1]->seq_region_name()); };
+ } else {
+ $slice_setter = sub{} if(!$slice_setter);
+ }
+ }
+
+ return $slice_setter;
+}
+
+
=head2 fetch_all_by_RawContig
Arg [1] : Bio::EnsEMBL::RawContig $contig
Example : @features = @{$self->fetch_all_by_RawContig($contig)};
- Description: Retrieves features on the region defined by the $contig arg in
- RawContig coordinates.
+ Description: You should use the fetch_all_by_Slice method instead of this
+ method now. RawContigs have been effectively replaced by
+ Slices.
If this method is overridden then it is also necessary to
override the fetch_all_by_contig_name method due to
@@ -344,172 +455,124 @@ sub fetch_all_by_Slice {
=cut
sub fetch_all_by_RawContig {
- my ($self, $contig) = @_;
+ my $self = shift;
+ return $self->fetch_all_by_Slice(@_);
+}
- unless($contig && ref $contig && $contig->isa('Bio::EnsEMBL::RawContig')) {
- $self->throw("[$contig] is not a Bio::EnsEMBL::RawContig");
- }
- my $out = [];
+=head2 fetch_all_by_contig_name
- if($self->_supported('CONTIG')) {
- return $self->fetch_all_by_contig_name($contig->name);
- }
+ Arg [1] : string $contig_name
+ Arg [2] : int $start (optional)
+ The start of the region on the contig to retrieve features on
+ if not specified the whole of the contig is used.
+ Arg [3] : int $end (optional)
+ The end of the region on the contig to retrieve features on
+ if not specified the whole of the contig is used.
+ Example : @fs = @{$self->fetch_all_by_contig_name('AB00879.1.1.39436')};
+ Description: Retrieves features on the contig defined by the name
+ $contig_name in RawContig coordinates.
- if($self->_supported('CLONE')) {
- #retrieve features in clone coordinates
+ If this method is overridden then the coordinate_systems
+ method must return 'CONTIG' as one of its values.
- my $offset = $contig->embl_offset;
- my $length = $contig->length;
+ This method will work as is (i.e. without being overridden)
+ providing at least one other fetch method has
+ been overridden.
+ Returntype : reference to a list of Bio::SeqFeature objects in the RawContig
+ coordinate system.
+ Exceptions : thrown if the input argument is incorrect
+ thrown if the coordinate_systems method returns the value
+ 'CONTIG' and this method has not been overridden.
+ Caller : general, fetch_all_by_Slice
- my $feats = $self->fetch_all_by_Clone($contig->clone, $offset,
- $offset + $length - 1);
-
- my ($start, $end);
-
- #convert from clone coordinates to contig coordinates
- foreach my $f (@$feats) {
- $start = $f->start - $offset + 1;
- $end = $f->end - $offset + 1;
-
- #skip features which are not entirely on this contig
- next if($start < 1 || $end > $length);
-
- $f->start($start);
- $f->end($end);
- $f->attach_seq($contig);
- push(@$out, $f);
- }
+=cut
+
+sub fetch_all_by_contig_name {
+ my ($self, $contig_name, $start, $end) = @_;
- return $out;
+ unless($contig_name) {
+ throw("contig_name argument not defined");
}
- unless($self->_supported('SLICE') || $self->_supported('ASSEMBLY')) {
- $self->_no_valid_coord_system;
+ if($self->_supported('CONTIG')) {
+ throw("ExternalFeatureAdaptor supports CONTIG coordinate system" .
+ " but fetch_all_by_contig_name is not implemented");
}
unless($self->ensembl_db) {
- $self->throw('DB attribute not set. This value must be set for the ' .
+ throw('DB attribute not set. This value must be set for the ' .
'ExternalFeatureAdaptor to function correctly');
}
- my $asma = $self->ensembl_db->get_AssemblyMapperAdaptor;
- my $mapper = $asma->fetch_by_type($self->ensembl_db->assembly_type);
-
- #map the whole contig to the assembly
- my @mapped = $mapper->map_coordinates_to_assembly($contig->dbID, 1,
- $contig->length, 1);
-
- my $chr;
-
- foreach my $coord (@mapped) {
- #skip over parts of the contig that do not map to the assembly
- next if ($coord->isa('Bio::EnsEMBL::Mapper::Gap'));
-
- $chr = $coord->id;
-
- #retrieve the features in assembly coordinates
- push @$out, @{$self->fetch_all_by_chr_start_end($coord->id,
- $coord->start,
- $coord->end)};
- }
-
- #map each feature from assembly coords back to raw contig coords
- foreach my $f (@$out) {
- @mapped =$mapper->map_coordinates_to_rawcontig($chr,
- $f->start,
- $f->end,
- $f->strand);
-
- #skip if feature does not map cleanly to our contig
- next if(@mapped != 1);
- next if($mapped[0]->isa('Bio::EnsEMBL::Mapper::Gap'));
-
- unless($mapped[0]->id == $contig->dbID) {
- $self->throw('Error mapping feature from assembly to contig');
- }
-
- $f->start ($mapped[0]->start);
- $f->end ($mapped[0]->end);
- $f->strand($mapped[0]->strand);
- $f->attach_seq($contig);
- }
-
- return $out;
+ my $slice_adaptor = $self->ensembl_db->get_SliceAdaptor();
+ my $slice = $slice_adaptor->fetch_by_region('contig', $contig_name,
+ $start, $end);
+ return $self->fetch_all_by_Slice($slice);
}
-=head2 fetch_all_by_contig_name
- Arg [1] : string $contig_name
- Example : @fs = @{$self->fetch_all_by_contig_name('AB00879.1.1.39436')};
+=head2 fetch_all_by_supercontig_name
+
+ Arg [1] : string $supercontig_name
+ Arg [2] : int $start (optional)
+ The start of the region on the contig to retrieve features on
+ if not specified the whole of the contig is used.
+ Arg [3] : int $end (optional)
+ The end of the region on the contig to retrieve features on
+ if not specified the whole of the contig is used.
+ Example : @fs = @{$self->fetch_all_by_contig_name('NT_004321')};
Description: Retrieves features on the contig defined by the name
- $contig_name in RawContig coordinates.
+ $supercontigname in supercontig coordinates.
If this method is overridden then the coordinate_systems
- method must return 'CONTIG' as one of its values. If the
- fetch_all_by_Contig method is overridden then this method
- should also be overridden to chain calls to that method (and
- avoid throwing an exception when this method is called).
+ method must return 'SUPERCONTIG' as one of its values.
This method will work as is (i.e. without being overridden)
providing at least one other fetch method has
- been overridden and the fetch_all_by_Contig method has not been
- overridden.
+ been overridden.
Returntype : reference to a list of Bio::SeqFeature objects in the RawContig
coordinate system.
Exceptions : thrown if the input argument is incorrect
thrown if the coordinate_systems method returns the value
- 'CONTIG' and this method has not been overridden.
- Caller : general, fetch_all_by_Contig
+ 'SUPERCONTIG' and this method has not been overridden.
+ Caller : general, fetch_all_by_Slice
=cut
-sub fetch_all_by_contig_name {
- my ($self, $contig_name) = @_;
- unless($contig_name) {
- $self->throw("contig_name argument not defined");
+sub fetch_all_by_supercontig_name {
+ my ($self, $supercontig_name, $start, $end) = @_;
+
+ unless($supercontig_name) {
+ throw("supercontig_name argument not defined");
}
- if($self->_supported('CONTIG')) {
- $self->throw("ExternalFeatureAdaptor supports CONTIG coordinate system" .
- " but fetch_all_by_contig_name is not implemented");
+ if($self->_supported('SUPERCONTIG')) {
+ throw("ExternalFeatureAdaptor supports SUPERCONTIG coordinate system" .
+ " but fetch_all_by_supercontig_name is not implemented");
}
unless($self->ensembl_db) {
- $self->throw('DB attribute not set. This value must be set for the ' .
+ throw('DB attribute not set. This value must be set for the ' .
'ExternalFeatureAdaptor to function correctly');
}
- my $contig_adaptor = $self->ensembl_db->get_RawContigAdaptor;
- my $contig = $contig_adaptor->fetch_by_name($contig_name);
-
- unless($contig) {
- $self->warn("ExternalFeatureAdaptor::fetch_all_by_contig_name: Contig " .
- "[$contig_name] not found\n");
- return [];
- }
-
-
- return $self->fetch_all_by_RawContig($contig);
+ my $slice_adaptor = $self->ensembl_db->get_SliceAdaptor();
+ my $slice = $slice_adaptor->fetch_by_region('supercontig', $supercontig_name,
+ $start, $end);
+ return $self->fetch_all_by_Slice($slice);
}
-
-
=head2 fetch_all_by_Clone
Arg [1] : Bio::EnsEMBL::Clone $clone
- Arg [2] : (optional) int $clone_start the start of the clonal region
- interested in. This information may be used to speed up
- the query, or may be ignored.
- Arg [3] : (optional) int $clone_end the end of the clonal region
- interested in. This information may be used to speed up
- the query, or may be ignored.
Example : @features = @{$self->fetch_all_by_Clone($clone)};
- Description: Retrieves features on the region defined by the $clone arg in
- Clone coordinates.
+ Description: You should not use this method anymore. Use the
+ fetch_all_by_Slice method instead. Clones have effectively been
+ replaced by Slices on the 'clone' coordinate system.
If this method is overridden then it is also necessary to
override the fetch_all_by_clone_accession method due to
@@ -532,44 +595,8 @@ sub fetch_all_by_contig_name {
=cut
sub fetch_all_by_Clone {
- my ($self, $clone, $clone_start, $clone_end) = @_;
-
- #ignore $clone_start && $clone_end it is only useful when
- #fetch_all_by_RawContig is not implemented, but fetch_all_by_Clone is
- #i.e. it can be used by people who override this method...
-
- unless($clone && ref $clone && $clone->isa('Bio::EnsEMBL::Clone')) {
- $self->throw("Clone must be a Bio::EnsEMBL::Clone");
- }
-
- if($self->_supported('CLONE')) {
- return $self->fetch_all_by_clone_accession($clone->id, $clone->embl_acc,
- $clone_start, $clone_end);
- }
-
- unless($self->_supported('CONTIG') ||
- $self->_supported('SLICE') ||
- $self->_supported('ASSEMBLY')) {
- $self->_no_valid_coord_system;
- }
-
- my $out = [];
-
- #retrieve features from each contig in the clone
- my $contigs = $clone->get_all_Contigs;
- my $offset;
- foreach my $contig (@$contigs) {
- $offset = $contig->embl_offset;
- foreach my $f (@{$self->fetch_all_by_RawContig($contig)}) {
- #convert each feature to clone coordinates
- $f->start($f->start - $offset + 1);
- $f->end($f->end - $offset + 1);
- #$f->attach_seq($clone); #this might work in future...
- push @$out, $f;
- }
- }
-
- return $out;
+ my $self = shift;
+ return $self->fetch_all_by_Slice(@_);
}
@@ -614,32 +641,30 @@ sub fetch_all_by_clone_accession {
my ($self, $acc, $version, $start, $end) = @_;
unless($acc) {
- $self->throw("clone accession argument not defined");
+ throw("clone accession argument not defined");
}
if($self->_supported('CLONE')) {
- $self->throw('ExternalFeatureAdaptor supports CLONE coordinate system ' .
+ throw('ExternalFeatureAdaptor supports CLONE coordinate system ' .
'but does not implement fetch_all_by_clone_accession');
}
unless($self->ensembl_db) {
- $self->throw('DB attribute not set. This value must be set for the ' .
+ throw('DB attribute not set. This value must be set for the ' .
'ExternalFeatureAdaptor to function correctly');
}
+ if(defined($version)) {
+ $acc = "$acc.$version";
+ } elsif(!$acc =~ /\./) {
+ $acc = "$acc.1";
+ }
- my $clone_adaptor = $self->ensembl_db->get_CloneAdaptor;
- my $clone = $clone_adaptor->fetch_by_accession($acc);
-
+ my $slice_adaptor = $self->ensembl_db->get_SliceAdaptor;
- unless($clone) {
- $self->warn("ExternalFeatureAdaptor::fetch_all_by_clone_accession: Clone "
- . "[$acc] not found\n");
- return [];
- }
-
+ my $slice = $slice_adaptor->fetch_by_region('clone', $acc, $start, $end);
- return $self->fetch_all_by_Clone($clone);
+ return $self->fetch_all_by_Slice($slice);
}
@@ -675,85 +700,20 @@ sub fetch_all_by_chr_start_end {
my ($self, $chr_name, $start, $end) = @_;
unless($chr_name && defined $start && defined $end && $start < $end) {
- $self->throw("Incorrect start [$start] end [$end] or chr [$chr_name] arg");
+ throw("Incorrect start [$start] end [$end] or chr [$chr_name] arg");
}
unless($self->ensembl_db) {
- $self->throw('DB attribute not set. This value must be set for the ' .
+ throw('DB attribute not set. This value must be set for the ' .
'ExternalFeatureAdaptor to function correctly');
}
- my $out = [];
-
- my $asma = $self->ensembl_db->get_AssemblyMapperAdaptor;
- my $mapper = $asma->fetch_by_type($self->ensembl_db->assembly_type);
- my $contig_adaptor = $self->ensembl_db->get_RawContigAdaptor;
-
- if($self->_supported('ASSEMBLY')) {
- $self->throw("ExternalFeatureAdaptor supports ASSEMBLY coordinate system".
- " but fetch_all_by_chr_start_end is not implemented");
-
- }
-
- my $slice_adaptor = $self->ensembl_db->get_SliceAdaptor;
- #get a slice of the whole chromosome
- my $chrom_slice = $slice_adaptor->fetch_by_chr_name($chr_name);
+ my $slice_adaptor = $self->ensembl_db->get_SliceAdaptor();
- if($self->_supported('SLICE')) {
-
- #fetch by slice and convert to assembly coords
- my $slice = $slice_adaptor->fetch_by_chr_start_end($chr_name,$start,$end);
- $out = $self->fetch_all_by_Slice($slice);
-
- foreach my $f (@$out) {
- $f->start($start + $f->start - 1);
- $f->end ($start + $f->end - 1);
- $f->attach_seq($chrom_slice);
- }
+ my $slice = $slice_adaptor->fetch_by_region('toplevel', $chr_name, $start,
+ $end);
- return $out;
- }
-
- #fetch via rawcontig and convert to assembly coords
-
- #Figure out what contigs we are overlapping
- my @cids = $mapper->list_contig_ids( $chr_name, $start, $end);
-
- foreach my $cid (@cids) {
- my $contig = $contig_adaptor->fetch_by_dbID($cid);
- #retrieve features of each contig that we are overlapping
- my $feats = $self->fetch_all_by_RawContig($contig);
-
- foreach my $f (@$feats) {
- #convert each feature from contig coords to assembly coords
- my @mapped = $mapper->map_coordinates_to_assembly($cid,
- $f->start,
- $f->end,
- $f->strand);
-
- #if maps to multiple locations in assembly, skip feature
- next if(@mapped > 1);
-
- #if maps to a gap, skip
- next if($mapped[0]->isa('Bio::EnsEMBL::Mapper::Gap'));
-
- my $m_start = $mapped[0]->start;
- my $m_end = $mapped[0]->end;
- my $m_strand = $mapped[0]->strand;
-
- #skip features which do not overlap this assembly region
- next if($m_start > $end || $m_end < $start);
-
- $f->start($m_start);
- $f->end($m_end);
- $f->strand($m_strand);
- $f->attach_seq($chrom_slice);
-
- push @$out, $f;
- }
- }
-
- return $out;
+ return $self->fetch_all_by_Slice($slice);
}
@@ -771,9 +731,9 @@ sub fetch_all_by_chr_start_end {
sub _no_valid_coord_system {
my $self = shift;
- $self->throw("This ExternalFeatureAdaptor does not support a known " .
+ throw("This ExternalFeatureAdaptor does not support a known " .
"coordinate system.\n Valid coordinate systems are: " .
- "[SLICE, ASSEMBLY, CONTIG, CLONE].\n This External Adaptor " .
+ "[SLICE,ASSEMBLY,SUPERCONTIG,CONTIG,CLONE].\n This External Adaptor " .
"supports: [" . join(', ', $self->coordinate_systems) . "]");
}
diff --git a/modules/Bio/EnsEMBL/Feature.pm b/modules/Bio/EnsEMBL/Feature.pm
new file mode 100644
index 0000000000..7002445b98
--- /dev/null
+++ b/modules/Bio/EnsEMBL/Feature.pm
@@ -0,0 +1,767 @@
+# EnsEMBL module for Bio::EnsEMBL::Feature
+#
+# Copyright (c) 2003 EnsEMBL
+#
+
+
+=head1 NAME
+
+Bio::EnsEMBL::Feature - Ensembl specific sequence feature.
+
+=head1 SYNOPSIS
+
+ my $feat = new Bio::EnsEMBL::Feature(-start => 100,
+ -end => 220,
+ -strand => -1,
+ -slice => $slice
+ -analysis => $analysis
+ );
+
+ my $start = $feat->start;
+ my $end = $feat->end;
+ my $strand = $feat->strand;
+
+ #move the feature to the chromosomal coordinate system
+ $feature = $feature->transform('chromosome');
+
+ #move the feature to a different slice (possibly on another coord system)
+ $feature = $feature->transfer($new_slice);
+
+ #project the feature onto another coordinate system possibly across
+ #boundaries:
+ @projection = @{$feature->project('contig')};
+
+ #change the start, end, and strand of the feature in place
+ $feature->move($new_start, $new_end, $new_strand);
+
+=head1 DESCRIPTION
+
+This is the Base feature class from which all EnsEMBL features inherit. It
+provides a bare minimum functionality that all features require. It basically
+describes a location on a sequence in an arbitrary coordinate system.
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL development list: ensembl-dev@ebi.ac.uk
+
+=head1 METHODS
+
+=cut
+
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::Feature;
+
+use Bio::EnsEMBL::Storable;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate warning);
+use Bio::EnsEMBL::Slice;
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::Storable);
+
+
+=head2 new
+
+ Arg [-SLICE]: Bio::EnsEMBL::SLice - Represents the sequence that this
+ feature is on. The coordinates of the created feature are
+ relative to the start of the slice.
+ Arg [-START]: The start coordinate of this feature relative to the start
+ of the slice it is sitting on. Coordinates start at 1 and
+ are inclusive.
+ Arg [-END] : The end coordinate of this feature relative to the start of
+ the slice it is sitting on. Coordinates start at 1 and are
+ inclusive.
+ Arg [-STRAND]: The orientation of this feature. Valid values are 1,-1,0.
+ Arg [-SEQNAME] : A seqname to be used instead of the default name of the
+ of the slice. Useful for features that do not have an
+ attached slice such as protein features.
+ Example : $feature = Bio::EnsEMBL::Feature->new(-start => 1,
+ -end => 100,
+ -strand => 1,
+ -slice => $slice,
+ -analysis => $analysis);
+ Description: Constructs a new Bio::EnsEMBL::Feature. Generally subclasses
+ of this method are instantiated, rather than this class itself.
+ Returntype : Bio::EnsEMBL::Feature
+ Exceptions : Thrown on invalid -SLICE, -ANALYSIS, -STRAND arguments
+ Caller : general, subclass constructors
+
+=cut
+
+
+sub new {
+ my $caller = shift;
+
+ my $class = ref($caller) || $caller;
+
+ my($start, $end, $strand, $slice, $analysis,$seqname, $dbID, $adaptor) =
+ rearrange(['START','END','STRAND','SLICE','ANALYSIS', 'SEQNAME',
+ 'DBID', 'ADAPTOR'], @_);
+
+ if($slice) {
+ if(!ref($slice) || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw('-SLICE argument must be a Bio::EnsEMBL::Slice not '.$slice);
+ }
+ }
+
+ if($analysis) {
+ if(!ref($analysis) || !$analysis->isa('Bio::EnsEMBL::Analysis')) {
+ throw('-ANALYSIS argument must be a Bio::EnsEMBL::Analysis not '.
+ $analysis);
+ }
+ }
+
+ if(defined($strand)) {
+ if(!($strand == 1) && !($strand == -1) && !($strand == 0)) {
+ throw('-STRAND argument must be 1, -1, or 0');
+ }
+ }
+
+ if(defined($start) && defined($end)) {
+ if($end < $start) {
+ throw('-START argument must be less than -END argument');
+ }
+ }
+
+ return bless({'start' => $start,
+ 'end' => $end,
+ 'strand' => $strand,
+ 'slice' => $slice,
+ 'analysis' => $analysis,
+ 'adaptor' => $adaptor,
+ 'seqname' => $seqname,
+ 'dbID' => $dbID}, $class);
+}
+
+
+
+=head2 start
+
+ Arg [1] : (optional) int $start
+ The start of this feature relative to the start of the slice
+ that it is on.
+ Example : $start = $feat->start()
+ Description: Getter/Setter for the start of this feature relative to the
+ start of the slice it is on. Note that negative values, or
+ values exceeding the length of the slice are permitted.
+ Start must be less than or equal to the end regardless of the
+ strand. Coordinate values start at 1 and are inclusive.
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub start {
+ my $self = shift;
+ $self->{'start'} = shift if(@_);
+ return $self->{'start'};
+}
+
+
+
+
+=head2 end
+
+ Arg [1] : (optional) int $end
+ Example : $end = $feat->end();
+ Description: Getter/Setter for the end of this feature relative to the
+ start of the slice that it is on. Note that negative values,
+ of values exceeding the length of the slice are permitted. End
+ must be greater than or equal to start regardless of the strand.
+ Coordinate values start at 1 and are inclusive.
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub end {
+ my $self = shift;
+ $self->{'end'} = shift if(@_);
+ return $self->{'end'};
+}
+
+
+
+
+=head2 strand
+
+ Arg [1] : (optional) int $strand
+ Example : $feat->strand(-1);
+ Description: Getter/Setter for the strand of this feature relative to the
+ slice it is on. 0 is an unknown or non-applicable strand.
+ -1 is the reverse (negative) strand and 1 is the forward
+ (positive) strand. No other values are permitted.
+ Returntype : int
+ Exceptions : thrown if an invalid strand argument is passed
+ Caller : general
+
+=cut
+
+sub strand {
+ my $self = shift;
+
+ if(@_) {
+ my $strand = shift || 0;
+ if(defined($strand) && $strand != 0 && $strand != 1 && $strand != -1) {
+ throw('strand argument must be 0, -1 or 1');
+ }
+
+ $self->{'strand'} = $strand;
+ }
+
+ return $self->{'strand'};
+}
+
+
+
+=head2 move
+
+ Arg [1] : int start
+ Arg [2] : int end
+ Arg [3] : (optional) int strand
+ Example : None
+ Description: Sets the start, end and strand in one call rather than in
+ 3 seperate calls to the start(), end() and strand() methods.
+ This is for convenience and for speed when this needs to be
+ done within a tight loop.
+ Returntype : none
+ Exceptions : Thrown is invalid arguments are provided
+ Caller : general
+
+=cut
+
+sub move {
+ my $self = shift;
+
+ throw('start and end arguments are required') if(@_ < 2);
+
+ my $start = shift;
+ my $end = shift;
+ my $strand = shift;
+
+ if(defined($start) && defined($end) && $end < $start) {
+ throw('start must be less than or equal to end');
+ }
+ if(defined($strand) && $strand != 0 && $strand != -1 && $strand != 1) {
+ throw('strand must be 0, -1 or 1');
+ }
+
+ $self->{'start'} = $start;
+ $self->{'end'} = $end;
+ $self->{'strand'} = $strand if(defined($strand));
+}
+
+
+
+=head2 length
+
+ Arg [1] : none
+ Example : $length = $feat->length();
+ Description: Returns the length of this feature
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub length {
+ my $self = shift;
+ return $self->{'end'} - $self->{'start'} + 1;
+}
+
+
+
+
+=head2 analysis
+
+ Arg [1] : (optional) Bio::EnsEMBL::Analysis $analysis
+ Example : $feature->analysis(new Bio::EnsEMBL::Analysis(...))
+ Description: Getter/Setter for the analysis that is associated with
+ this feature. The analysis describes how this feature
+ was derived.
+ Returntype : Bio::EnsEMBL::Analysis
+ Exceptions : thrown if an invalid argument is passed
+ Caller : general
+
+=cut
+
+sub analysis {
+ my $self = shift;
+
+ if(@_) {
+ my $an = shift;
+ if(defined($an) && (!ref($an) || !$an->isa('Bio::EnsEMBL::Analysis'))) {
+ throw('analysis argument must be a Bio::EnsEMBL::Analysis');
+ }
+ $self->{'analysis'} = $an;
+ }
+
+ return $self->{'analysis'};
+}
+
+
+
+=head2 slice
+
+ Arg [1] : (optional) Bio::EnsEMBL::Slice $slice
+ Example : $seqname = $feature->slice()->name();
+ Description: Getter/Setter for the Slice that is associated with this
+ feature. The slice represents the underlying sequence that this
+ feature is on. Note that this method call is analagous to the
+ old SeqFeature methods contig(), entire_seq(), attach_seq(),
+ etc.
+ Returntype : Bio::EnsEMBL::Slice
+ Exceptions : thrown if an invalid argument is passed
+ Caller : general
+
+=cut
+
+sub slice {
+ my $self = shift;
+
+ if(@_) {
+ my $sl = shift;
+ if(defined($sl) && (!ref($sl) || !$sl->isa('Bio::EnsEMBL::Slice'))) {
+ throw('slice argument must be a Bio::EnsEMBL::Slice');
+ }
+
+ $self->{'slice'} = $sl;
+ }
+
+ return $self->{'slice'};
+}
+
+
+=head2 transform
+
+ Arg [1] : string $coord_system
+ The coord system to transform this feature to.
+ Arg [2] : string $version (optional)
+ The version of the coord system to transform this feature to.
+ Example : $feature = $feature->transform('contig');
+ next if(!defined($feature));
+ Description: Returns a copy of this feature, but converted to a different
+ coordinate system. The converted feature will be placed on a
+ slice which spans an entire sequence region of the new
+ coordinate system. If the requested coordinate system is the
+ same coordinate system it is simply placed on a slice which
+ spans the entire seq_region (as opposed to the original slice
+ which may have only partially covered the seq_region).
+
+ If a feature spans a boundary in the new coordinate system,
+ undef is returned instead.
+
+ For example, transforming an exon in contig coordinates to one
+ in chromosomal coodinates will place the exon on a slice of an
+ entire chromosome.
+ Returntype : Bio::EnsEMBL::Feature (or undef)
+ Exceptions : thrown if an invalid coordinate system is provided
+ thrown if this feature is not already on a slice
+ Caller : general, transfer()
+
+=cut
+
+sub transform {
+ my $self = shift;
+ my $cs_name = shift;
+ my $cs_version = shift;
+
+ #
+ # For backwards compatibility check if the arguments are old style args
+ #
+ if(!$cs_name || ref($cs_name)) {
+ deprecate('Calling transform without a coord system name is deprecated.');
+ return $self->_deprecated_transform($cs_name);
+ }
+
+ my $slice = $self->{'slice'};
+
+ if(!$slice) {
+ throw('Feature is not associated with a slice and may not be transformed');
+ }
+
+
+ #use db from slice since this feature may not yet be stored in a database
+ my $db = $slice->adaptor->db();
+ my $cs = $db->get_CoordSystemAdaptor->fetch_by_name($cs_name, $cs_version);
+ my $current_cs = $slice->coord_system();
+
+ # self is already in the requested coordinate system, so we can just return a copy
+ if( $cs->equals( $current_cs ) && $slice->start() == 1 && $slice->strand() == 1 ) {
+ my $new_feature;
+ %$new_feature = %$self;
+ bless $new_feature, ref $self;
+ return $new_feature;
+ }
+
+ my $projection = $self->project( $cs_name, $cs_version );
+ if( @$projection != 1 ) {
+ return undef;
+ } else {
+ my $p_slice = $projection->[0]->[2];
+ my $slice_adaptor = $db->get_SliceAdaptor;
+ $slice = $slice_adaptor->fetch_by_region($p_slice->coord_system()->name(),
+ $p_slice->seq_region_name(),
+ undef, #start
+ undef, #end
+ 1, #strand
+ $p_slice->coord_system()->version);
+
+ my $new_feature;
+ %$new_feature = %$self;
+ bless $new_feature, ref $self;
+ $new_feature->{'start'} = $p_slice->start();
+ $new_feature->{'end'} = $p_slice->end();
+ $new_feature->{'strand'} = ($self->{'strand'} == 0) ? 0 : $p_slice->strand();
+ $new_feature->{'slice'} = $slice;
+ return $new_feature;
+ }
+}
+
+
+
+=head2 transfer
+
+ Arg [1] : Bio::EnsEMBL::Slice $slice
+ The slice to transfer this feature to
+ Example : $feature = $feature->transfer($slice);
+ next if(!defined($feature));
+ Description: Returns a copy of this feature which has been shifted onto
+ another slice.
+
+ If the new slice is in a different coordinate system the
+ feature is transformed first and then placed on the slice.
+ If the feature would be split across a coordinate system
+ boundary or mapped to a gap undef is returned instead.
+ Returntype : Bio::EnsEMBL::Feature (or undef)
+ Exceptions : Thrown if the feature cannot be placed on the the same
+ seq_region as the slice.
+ Caller : general, transform()
+
+=cut
+
+
+sub transfer {
+ my $self = shift;
+ my $slice = shift;
+
+ if(!$slice || !ref($slice) || !$slice->isa('Bio::EnsEMBL::Slice')) {
+ throw('Slice argument is required');
+ }
+
+ my $current_slice = $self->{'slice'};
+
+ if(!$current_slice) {
+ throw('Feature must be on a slice to be transfered.');
+ }
+
+ #make a shallow copy of the feature to be transfered
+ my $feature;
+ %{$feature} = %{$self};
+ bless $feature, ref($self);
+
+ my $cur_cs = $current_slice->coord_system();
+ my $dest_cs = $slice->coord_system();
+
+ #if we are not in the same coord system a transformation step is needed first
+ if(!$dest_cs->equals($cur_cs)) {
+ $feature = $feature->transform($dest_cs->name, $dest_cs->version);
+ return undef if(!defined($feature));
+ $current_slice = $feature->{'slice'};
+ }
+
+ if($current_slice->seq_region_name() ne $slice->seq_region_name()) {
+ throw('Feature is not on the seq_region of the slice to transfer to');
+ }
+
+ #if the current feature positions are not relative to the start of the
+ #seq region, convert them so they are
+ my $cur_slice_start = $current_slice->start();
+ my $cur_slice_strand = $current_slice->strand();
+ if($cur_slice_start != 1 || $cur_slice_strand != 1) {
+ my $fstart = $feature->{'start'};
+ my $fend = $feature->{'end'};
+
+ if($cur_slice_strand == 1) {
+ $feature->{'start'} = $fstart + $cur_slice_start - 1;
+ $feature->{'end'} = $fend + $cur_slice_start - 1;
+ } else {
+ my $cur_slice_end = $current_slice->end();
+ $feature->{'start'} = $cur_slice_end - $fend + 1;
+ $feature->{'end'} = $cur_slice_end - $fstart + 1;
+ $feature->{'strand'} *= -1;
+ }
+ }
+
+ my $fstart = $feature->{'start'};
+ my $fend = $feature->{'end'};
+
+ #convert to destination slice coords
+ if($slice->strand == 1) {
+ $feature->{'start'} = $fstart - $slice->start() + 1;
+ $feature->{'end'} = $fend - $slice->start() + 1;
+ } else {
+ $feature->{'start'} = $slice->end() - $fend + 1;
+ $feature->{'end'} = $slice->end() - $fstart + 1;
+ $feature->{'strand'} *= -1;
+ }
+
+ $feature->{'slice'} = $slice;
+
+ return $feature;
+}
+
+
+
+=head2 project
+
+ Arg [1] : string $name
+ The name of the coordinate system to project this feature onto
+ Arg [2] : string $version (optional)
+ The version of the coordinate system (such as 'NCBI34') to
+ project this feature onto
+ Example :
+ my $clone_projection = $feature->project('clone');
+
+ foreach my $segment (@$clone_projection) {
+ my ($start, $end, $clone) = @$segment;
+ print "Features current coords $start-$end project onto clone coords " .
+ $clone->seq_region_name, ':', $clone->start, '-', $clone->end,
+ $clone->strand, "\n";
+ }
+ Description: Returns the results of 'projecting' this feature onto another
+ coordinate system. This is useful to see where a feature
+ where a feature would lie in a coordinate system in which it
+ crosses a boundary.
+
+ This method returns a listref of triplets [start,end,slice]
+ which represents the projection. The start and end are the
+ coordinates relative to the feature start.
+ For example if a feature is current 100-200bp on a slice
+ then the triplets returned might be:
+ 1,50,$slice1
+ 51,101,$slice2
+
+ The third value of the returned triplets is a slice spanning
+ the region on the requested coordinate system that this feature
+ projected to.
+
+ If the feature projects entirely into a gap then a reference to
+ an empty list is returned.
+
+ Returntype : list reference of [$start,$end,$slice] triplets
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub project {
+ my $self = shift;
+ my $cs_name = shift;
+ my $cs_version = shift;
+
+ my $slice = $self->{'slice'};
+
+ if(!$slice) {
+ throw("Feature is not associated with a Slice and may not be projected");
+ }
+
+ #get an adaptor from the attached slice because this feature may not yet
+ #be stored and may not have its own adaptor
+ my $slice_adaptor = $slice->adaptor();
+
+ if(!$slice_adaptor) {
+ throw("Cannot project feature because associated slice does not have an " .
+ " adaptor");
+ }
+
+ my $strand = $self->strand() * $slice->strand();
+ #fetch by feature always gives back forward strand slice:
+ $slice = $slice_adaptor->fetch_by_Feature($self);
+ $slice = $slice->invert if($strand == -1);
+
+ return $slice->project($cs_name, $cs_version);
+}
+
+
+
+=head2 seqname
+
+ Arg [1] : (optional) $seqname
+ Example : $seqname = $feat->seqname();
+ Description: Getter/Setter for the name of the sequence that this feature
+ is on. Normally you can get away with not setting this value
+ and it will default to the name of the slice on which this
+ feature is on. It is useful to set this value on features which
+ do not ordinarily sit on features such as ProteinFeatures which
+ sit on peptides.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub seqname {
+ my $self = shift;
+
+ if(@_) {
+ $self->{'seqname'} = shift;
+ }
+
+ if(!$self->{'seqname'} && $self->slice()) {
+ return $self->slice->name();
+ }
+
+ return $self->{'seqname'};
+}
+
+
+
+
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $f->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. It is overridden by subclasses to
+ return an appropriate value for objects of that particular
+ class. If no appropriate display id is available an empty
+ string is returned instead.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
+
+=cut
+
+sub display_id {
+ my $self = shift;
+ return '';
+}
+
+
+##############################################
+# Methods included for backwards compatibility
+##############################################
+
+
+# contig
+#
+# This method is included for backwards compatibility.
+# Use slice() instead
+#
+sub contig {
+ deprecate('Use slice() instead');
+ slice(@_);
+}
+
+
+
+# sub_SeqFeature
+#
+# This method is only for genebuild backwards compatibility.
+# Avoid using it if possible
+#
+sub sub_SeqFeature{
+ my ($self) = @_;
+ return @{$self->{'_gsf_sub_array'}} if($self->{'_gsf_sub_array'});
+}
+
+# add_sub_SeqFeature
+#
+# This method is only for genebuild backwards compatibility.
+# Avoid using it if possible
+#
+sub add_sub_SeqFeature{
+ my ($self,$feat,$expand) = @_;
+
+ if( $expand eq 'EXPAND' ) {
+ # if this doesn't have start/end set - forget it!
+ if( !defined $self->start && !defined $self->end ) {
+ $self->start($feat->start());
+ $self->end($feat->end());
+ $self->strand($feat->strand);
+ } else {
+ my ($start,$end);
+ if( $feat->start < $self->start ) {
+ $start = $feat->start;
+ }
+
+ if( $feat->end > $self->end ) {
+ $end = $feat->end;
+ }
+
+ $self->start($start);
+ $self->end($end);
+ }
+ } else {
+ if($self->start > $feat->start || $self->end < $feat->end) {
+ throw("$feat is not contained within parent feature, " .
+ "and expansion is not valid");
+ }
+ }
+
+ push(@{$self->{'_gsf_sub_array'}},$feat);
+}
+
+# flush_sub_SeqFeature
+#
+# This method is only for genebuild backwards compatibility.
+# Avoid using it isf possible
+#
+sub flush_sub_SeqFeature {
+ my ($self) = @_;
+ $self->{'_gsf_sub_array'} = [];
+}
+
+
+sub _deprecated_transform {
+ my $self = shift;
+ my $arg = shift;
+
+ if(!$arg) {
+ warning("Calling transform() with no arguments is deprecated.\n".
+ "A coordinate system name argument should be used instead.\n".
+ "You probably wanted transform('seqlevel') or transform('contig').");
+ return $self->transform('seqlevel');
+ }
+
+ if(ref($arg) eq 'Bio::EnsEMBL::Slice') {
+ if($arg->{'empty'}) {
+ warning("Calling transform with an empty slice is deprecated.\n" .
+ "A coordinate system name argument should be used instead.\n".
+ "You probably wanted transform('chromosome') or " .
+ "transform('toplevel')");
+ return $self->transform('toplevel');
+ }
+ warning("Calling transform with a slice is deprecated.\n" .
+ "Use the transfer method instead");
+ return $self->transfer($arg);
+ }
+
+ warning("Calling transform with a [".ref($arg)."] arg is no longer " .
+ "(or never was) supported. Doing nothing instead.");
+
+ return $self;
+}
+
+# id
+#
+# This method is included for backwards compatibility only.
+# Use hseqname or dbID or stable_id instead
+#
+sub id {
+ my $self = shift;
+ deprecate("id method is not used - use display_id instead");
+ return $self->{'stable_id'} if($self->{'stable_id'});
+ return $self->{'hseqname'} if($self->{'hseqname'});
+ return $self->{'seqname'} if($self->{'seqname'});
+ return $self->{'dbID'};
+}
+
+1;
diff --git a/modules/Bio/EnsEMBL/FeaturePair.pm b/modules/Bio/EnsEMBL/FeaturePair.pm
index 0cad54569e..00dfbaa241 100755
--- a/modules/Bio/EnsEMBL/FeaturePair.pm
+++ b/modules/Bio/EnsEMBL/FeaturePair.pm
@@ -1,4 +1,3 @@
-
#
# BioPerl module for Bio::EnsEMBL::FeaturePair
#
@@ -6,399 +5,502 @@
#
# Copyright Ewan Birney
#
-# You may distribute this module under the same terms as perl itself
-
-# POD documentation - main docs before the code
+# You may distribute this module under the same terms as perl
=head1 NAME
-Bio::EnsEMBL::FeaturePair - Stores sequence features which are
- themselves hits to other sequence features.
+Bio::EnsEMBL::FeaturePair - Stores sequence Features which are
+themselves hits to other sequence features.
=head1 SYNOPSIS
- my $feat = new Bio::EnsEMBL::FeaturePair(-feature1 => $f1,
- -feature2 => $f2,
- );
-
- # Bio::SeqFeatureI methods can be used
- my $start = $feat->start;
- my $end = $feat->end;
-
- # Bio::EnsEMBL::SeqFeatureI methods can be used
- my $analysis = $feat->analysis;
-
- $feat->validate || $feat->throw("Invalid data in $feat");
-
- # Bio::FeaturePair methods can be used
- my $hstart = $feat->hstart;
- my $hend = $feat->hend;
+ my $feat = Bio::EnsEMBL::FeaturePair->new(-start => 132_231,
+ -end => 132_321,
+ -strand => -1,
+ -slice => $slice,
+ -hstart => 10,
+ -hend => 100,
+ -hstrand => 1,
+ -score => 100,
+ -percent_id => 92.0,
+ -hseqname => 'ALUSX10.1',
+ -analysis => $analysis);
+
+ my $hit_start = $feat->hstart();
+ my $hit_end = $feat->hend();
+ my $hit_strand = $feat->hstrand();
+ my $analysis = $feat->analysis();
=head1 DESCRIPTION
A sequence feature object where the feature is itself a feature on another
sequence - e.g. a blast hit where residues 1-40 of a protein sequence
SW:HBA_HUMAN has hit to bases 100 - 220 on a genomic sequence HS120G22.
-The genomic sequence coordinates are used to create one sequence feature
-$f1 and the protein coordinates are used to create feature $f2.
-A FeaturePair object can then be made
+The genomic sequence coordinates are represented by the start, end, strand
+attributes while the protein (hit) coordinates are represented by the hstart,
+hend, hstrand attributes.
+
+ $clone = $slice_adpator->fetch_by_region('clone', 'HS120G22');
+
+ $fp = Bio::EnsEMBL::FeaturePair(-start => 100,
+ -end => 220,
+ -strand => 1,
+ -slice => $clone,
+ -hstart => 1,
+ -hend => 40,
+ -hstrand => 1,
+ -percent_id => 92.0,
+ -score => 100,
+ -hseqname => 'SW:HBA_HUMAN',
+ -species => 'Homo sapiens',
+ -hspecies => 'Homo sapiens');
- my $fp = new Bio::EnsEMBL::FeaturePair(-feature1 => $f1, # genomic
- -feature2 => $f2, # protein
- );
-This object can be used as a standard Bio::SeqFeatureI in which case
+=head1 CONTACT
- my $gstart = $fp->start # returns start coord on feature1 - genomic seq.
- my $gend = $fp->end # returns end coord on feature1.
+Post comments/questions to the EnsEMBL developer list: ensembl-dev@ebi.ac.uk
-In general standard Bio::SeqFeatureI method calls return information
-in feature1.
+=head1 APPENDIX
-Data in the feature 2 object are generally obtained using the standard
-methods prefixed by h (for hit!)
+The rest of the documentation details each of the object methods.
+Internal methods are usually preceded with a _
- my $pstart = $fp->hstart # returns start coord on feature2 = protein seq.
- my $pend = $fp->hend # returns end coord on feature2.
+=cut
+package Bio::EnsEMBL::FeaturePair;
-If you wish to swap feature1 and feature2 around :
+use vars qw(@ISA);
+use strict;
- $feat->invert
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate warning);
+
+@ISA = qw(Bio::EnsEMBL::Feature);
+
+
+
+=head2 new
+
+ Arg [HSTART] : int - The start of the hit region (optional)
+ Arg [HEND] : int - The end of the hit region (optional)
+ Arg [HSTRAND] : (0,1,-1) - The strand of the hit region (optional)
+ Arg [PERCENT_ID]: float - The precentage identity of the hit (optional)
+ Arg [SCORE] : float - The score of the hit (optional)
+ Arg [HSEQNAME] : string - The name of the hit sequence (optional)
+ Arg [P_VALUE] : float - The pvalue or evalue (optional)
+ Arg [SPECIES] : string - The species the query sequence is from (optional)
+ Arg [HSPECIES] : string - The species the hit sequence is from (optional)
+ Arg [...] : Named superclass constructor args (Bio::EnsEMBL::Feature)
+ Example : $feat = Bio::EnsEMBL::FeaturePair->new(-start => 132_231,
+ -end => 132_321,
+ -strand => -1,
+ -slice => $slice,
+ -hstart => 10,
+ -hend => 100,
+ -hstrand => 1,
+ -score => 100,
+ -percent_id => 92.0,
+ -hseqname => 'ALUSX10.1',
+ -analysis => $analysis);
+Description: Creates a new Bio::EnsEMBL::FeaturePair object
+ Returntype : Bio::EnsEMBL::FeaturePair
+ Exceptions : throw if start > end
+ throw if invalid strand is provided
+ Caller : general
- $feat->start # etc. returns data in $feature2 object
+=cut
+sub new {
+ my $caller = shift;
-=head1 CONTACT
+ my $class = ref($caller) || $caller;
-Describe contact details here
+ my $self = $class->SUPER::new(@_);
-=head1 APPENDIX
+ my ($hstart,$hend,$hstrand,$percent_id,$score, $species, $hspecies,
+ $p_value, $hseqname, $f1,$f2) =
+ rearrange(['HSTART','HEND','HSTRAND','PERCENT_ID','SCORE','SPECIES',
+ 'HSPECIES', 'P_VALUE', 'HSEQNAME', 'FEATURE1','FEATURE2'], @_);
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
+ if(defined($hstart) && defined($hend) && ($hend < $hstart)) {
+ throw('HSTART must be less than or equal to HEND');
+ }
-=cut
+ if(defined($hstrand) && $hstrand != 1 && $hstrand != -1 && $hstrand != 0) {
+ throw('HSTRAND must be one of (0,1,-1)');
+ }
+
+ $self->{'hstart'} = $hstart;
+ $self->{'hend'} = $hend;
+ $self->{'hstrand'} = $hstrand;
+ $self->{'score'} = $score;
+ $self->{'percent_id'} = $percent_id;
+ $self->{'species'} = $species;
+ $self->{'hspecies'} = $hspecies;
+ $self->{'hseqname'} = $hseqname;
+ $self->{'p_value'} = $p_value;
+
+ #
+ # Feature1 and Feature2 arg handling for backwards compatibility
+ #
+ if($f1) {
+ deprecate("Using FEATURE1 arg to construct FeaturePairs" .
+ " is deprecated.\nUse the args START,END,STRAND,SLICE instead");
+
+ #eval because we are not exactly sure what f1 arg will look like
+ eval {
+ $self->{'start'} = $f1->start();
+ $self->{'end'} = $f1->end();
+ $self->{'strand'} = $f1->strand();
+ $self->{'slice'} = $f1->contig();
+ $self->{'analysis'} = $f1->analysis() if($f1->analysis());
+ };
+ }
+
+ if($f2) {
+ deprecate("Using FEATURE2 arg to construct FeaturePairs is deprecated" .
+ "\nUse the args HSTART,HEND,HSTRAND,HSEQNAME instead");
+
+ #eval because we are not exactly sure what f2 arg will look like
+ eval {
+ $self->{'hseqname'} = $f2->seqname();
+ $self->{'hstart'} = $f2->start();
+ $self->{'hend'} = $f2->end();
+ $self->{'hstrand'} = $f2->strand();
+ $self->{'analysis'} = $f2->analysis() if($f2->analysis());
+ };
+ }
+ return $self;
+}
-# Let the code begin...
-package Bio::EnsEMBL::FeaturePair;
+=head2 hseqname
-use vars qw(@ISA);
-use strict;
+ Arg [1] : string $hseqname (optional)
+ Example : $hseqname = $fp->hseqname();
+ Description: Getter/Setter for the name of the hit sequence
+ Returntype : string
+ Exceptions : none
+ Caller : general
-use Bio::EnsEMBL::SeqFeature;
+=cut
-@ISA = qw(Bio::EnsEMBL::SeqFeature);
+sub hseqname {
+ my $self = shift;
+ $self->{'hseqname'} = shift if(@_);
+ return $self->{hseqname};
+}
-sub new {
- my($class,@args) = @_;
- my $self = {};
- if( ref( $class) ) {
- $class = ref( $class );
- }
+=head2 hstart
- bless ($self,$class);
-
- my ($feature1,$feature2) =
- $self->_rearrange([qw(FEATURE1
- FEATURE2
- )],@args);
-
- if($feature1) {
- $self = $self->SUPER::new(-ANALYSIS => $feature1->analysis(),
- -SEQNAME => $feature1->seqname(),
- -START => $feature1->start(),
- -END => $feature1->end(),
- -STRAND => $feature1->strand(),
- -FRAME => $feature1->frame(),
- -SCORE => $feature1->score(),
- -PERCENT_ID => $feature1->percent_id(),
- -P_VALUE => $feature1->p_value(),
- -PHASE => $feature1->phase(),
- -END_PHASE => $feature1->end_phase());
-
- if($feature1->contig) {
- $self->contig($feature1->contig);
- }
- } else {
- $self = $self->SUPER::new( @args );
- }
+ Arg [1] : string $hstart (optional)
+ Example : $hstart = $fp->hstart();
+ Description: Getter/Setter for the start coordinate on the hit sequence
+ Returntype : int
+ Exceptions : none
+ Caller : general
- $feature2 && $self->feature2($feature2);
+=cut
- # set stuff in self from @args
- return $self; # success - we hope!
+sub hstart{
+ my $self = shift;
+ $self->{'hstart'} = shift if(@_);
+ return $self->{'hstart'};
}
-=head2 feature1
+=head2 hend
- Title : feature1
- Usage : $f = $featpair->feature1
- $featpair->feature1($feature)
- Function: Get/set for the query feature
- Returns : Bio::SeqFeatureI
- Args : none
+ Arg [1] : string $hend (optional)
+ Example : $hend = $fp->hend();
+ Description: Getter/Setter for the end coordinate on the hit sequence
+ Returntype : int
+ Exceptions : none
+ Caller : general
=cut
+sub hend{
+ my $self = shift;
+ $self->{'hend'} = shift if(@_);
+ return $self->{'hend'};
+}
+
-sub feature1 {
- my ($self,$arg) = @_;
- if($arg) {
- #print STDERR "have ".$arg." for feature1 and it has ".$arg->entire_seq."\n";
- $self->start($arg->start());
- $self->end($arg->end());
- $self->strand($arg->strand());
- $self->frame($arg->frame());
- $self->score($arg->score());
- $self->seqname($arg->seqname());
- $self->percent_id($arg->percent_id());
- $self->p_value($arg->p_value());
- $self->phase($arg->phase());
- $self->end_phase($arg->end_phase());
- $self->analysis($arg->analysis);
- if($arg->entire_seq){
- $self->attach_seq($arg->entire_seq);
- }
+=head2 hstrand
+
+ Arg [1] : int $hstrand (optional)
+ Example : $hstrand = $fp->hstrand
+ Description: Getter/Setter for the orientation of the hit on the hit sequence
+ Returntype : 0,1,-1
+ Exceptions : thrown
+ Caller : general
+
+=cut
+
+sub hstrand{
+ my $self = shift;
+
+ if(@_) {
+ my $hstrand = shift;
+ if(defined($hstrand) && $hstrand != 1 && $hstrand != 0 && $hstrand != -1) {
+ throw('hstrand must be one of (-1,0,1)');
}
+ $self->{'hstrand'} = $hstrand;
+ }
- return $self;
+ return $self->{'hstrand'};
}
-=head2 feature2
- Title : feature2
- Usage : $f = $featpair->feature2
- $featpair->feature2($feature)
- Function: Get/set for the hit feature
- Returns : Bio::SeqFeatureI
- Args : none
+=head2 score
+ Arg [1] : float $score (optional)
+ Example : $score = $fp->score();
+ Description: Getter/Setter for the score of this feature pair
+ Returntype : float
+ Exceptions : none
+ Caller : general
=cut
-sub feature2 {
- my ($self,$arg) = @_;
- #print "passing ".$arg." into feature2\n";
- if (defined($arg)) {
- unless(ref($arg) ne "" && $arg->isa("Bio::SeqFeatureI")) {
- $self->throw("Argument [$arg] must be a Bio::SeqFeatureI");
- }
-
- $self->hstart($arg->start());
- $self->hend($arg->end());
- $self->hstrand($arg->strand());
- $self->hseqname($arg->seqname());
- $self->hphase($arg->phase());
- $self->hend_phase($arg->end_phase());
-
- return $arg;
- }
-
- my $seq = new Bio::EnsEMBL::SeqFeature(
- -SEQNAME => $self->{_hseqname},
- -START => $self->{_hstart},
- -END => $self->{_hend},
- -STRAND => $self->{_hstrand},
- -SCORE => $self->score(),
- -PERCENT_ID => $self->percent_id(),
- -P_VALUE => $self->p_value(),
- -PHASE => $self->hphase,
- -END_PHASE => $self->hend_phase,
- -ANALYSIS => $self->analysis);
+sub score{
+ my $self = shift;
+ $self->{'score'} = shift if(@_);
+ return $self->{'score'};
+}
-
- return $seq;
+
+
+=head2 percent_id
+
+ Arg [1] : float $percent_id (optional)
+ Example : $percent_id = $fp->percent_id();
+ Description: Getter/Setter for the percentage identity of this feature pair
+ Returntype : float
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub percent_id {
+ my $self = shift;
+ $self->{'percent_id'} = shift if(@_);
+ return $self->{'percent_id'};
}
-=head2 hseqname
- Title : hseqname
- Usage : $featpair->hseqname($newval)
- Function: Get/set method for the name of
- feature2.
- Returns : value of $feature2->seqname
- Args : newvalue (optional)
+=head2 species
+ Arg [1] : string $genus_species_name (optional)
+ e.g. Homo_sapiens or Mus_musculus
+ Example : $species = $fp->species();
+ Description: get/set on the species of feature1
+ Returntype : string
+ Execeptions: none
+ Caller : general
=cut
-sub hseqname {
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
- # print STDERR "hseqname being set ".$arg."\n";
- $self->{_hseqname} = $arg;
- }
+sub species{
+ my $self = shift;
+ $self->{'species'} = shift if(@_);
+ return $self->{'species'};
+}
+
+
+=head2 hspecies
+
+ Arg [1] : string $genus_species_name (optional)
+ e.g. Homo_sapiens or Mus_musculus
+ Example : $hspecies = $fp->hspecies
+ Description: get/set on the species of feature2
+ Returntype : string
+ Execeptions: none
+ Caller : general
+
+=cut
- return $self->{_hseqname};
+sub hspecies{
+ my $self = shift;
+ $self->{'hspecies'} = shift if(@_);
+ return $self->{'hspecies'};
}
-=head2 hstart
- Title : hstart
- Usage : $start = $featpair->hstart
- $featpair->hstart(20)
- Function: Get/set on the start coordinate of feature2
- Returns : integer
- Args : none
+=head2 p_value
+
+ Arg [1] : float $p_value (optional)
+ Example : $eval = $fp->p_value
+ Description: Getter Setter for the evalue / pvalue of this feature
+ Returntype : float
+ Exceptions : none
+ Caller : general
=cut
-sub hstart {
- my ($self,$value) = @_;
-
- if (defined($value)) {
- #print STDERR "htart being set to ".$value,"\n";
- $self->{_hstart} = $value;
- }
-
- return $self->{_hstart};
+sub p_value{
+ my $self = shift;
+ $self->{'p_value'} = shift if(@_);
+ return $self->{'p_value'};
}
-=head2 hend
- Title : hend
- Usage : $end = $featpair->hend
- $featpair->hend($end)
- Function: get/set on the end coordinate of feature2
- Returns : integer
- Args : none
+
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $fp->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For feature pairs this is the
+ hseqname if it is available otherwise it is an empty string.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
=cut
-sub hend{
- my ($self,$value) = @_;
+sub display_id {
+ my $self = shift;
+ return $self->{'hseqname'} || '';
+}
- if (defined($value)) {
- #print STDERR "hend being set to ".$value."\n";
- $self->{_hend} = $value;
+
+###########################
+# DEPRECATED METHODS FOLLOW
+###########################
+
+=head2 feature1
+
+ Description: DEPRECATED use start(), end(), strand(), slice(), etc.
+ methods instead
+
+=cut
+
+sub feature1 {
+ my ($self,$arg) = @_;
+
+ deprecate('Use start(), end(), strand(), slice(), etc. methods instead.');
+
+ if($arg) {
+ $self->start($arg->start());
+ $self->end($arg->end());
+ $self->strand($arg->strand());
+ $self->score($arg->score());
+ $self->percent_id($arg->percent_id());
+ $self->analysis($arg->analysis);
+ if($arg->contig){
+ $self->slice($arg->contig);
}
+ }
- return $self->{_hend};
+ return $self;
}
-=head2 hstrand
+=head2 feature2
- Title : hstrand
- Usage : $strand = $feat->strand()
- $feat->strand($strand)
- Function: get/set on strand information, being 1,-1 or 0
- Returns : -1,1 or 0
- Args : none
+ Description: DEPRECATED use hstart(), hend(), hstrand() etc.
+ methods instead
=cut
-sub hstrand{
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
- #print "hstrand = ".$arg."\n";
- $self->{_hstrand} = $arg;
- }
-
- return $self->{_hstrand};
+sub feature2 {
+ my ($self,$arg) = @_;
+
+ deprecate('Use hstart(),hend(),hstrand(),hseqname() methods instead.');
+
+ if (defined($arg)) {
+ $self->hstart($arg->start());
+ $self->hend($arg->end());
+ $self->hstrand($arg->strand());
+ $self->hseqname($arg->seqname());
+ return $arg;
+ }
+
+ return new Bio::EnsEMBL::Feature(
+ -START => $self->hstart(),
+ -END => $self->hend(),
+ -STRAND => $self->hstrand(),
+ -SCORE => $self->score(),
+ -PERCENT_ID => $self->percent_id(),
+ -ANALYSIS => $self->analysis,
+ -SEQNAME => $self->hseqname());
}
=head2 invert
- Title : invert
- Usage : $tag = $feat->invert
- Function: Swaps feature1 and feature2 around
- Returns : Nothing
- Args : none
-
+ Description: DEPRECATED do not use
=cut
sub invert {
my ($self) = @_;
- my $tmp = $self->feature2;
+ deprecated('Do not use this method - it should not be needed');
+
+ my $hstart = $self->{'hstart'};
+ my $hend = $self->{'hend'};
+ my $hstrand = $self->{'hstrand'};
+ my $hspecies = $self->{'hspecies'};
+
+ my $start = $self->{'start'};
+ my $end = $self->{'end'};
+ my $strand = $self->{'strand'};
+ my $slice = $self->{'slice'};
+ my $species = $self->{'species'};
+
+ $self->{'start'} = $hstart;
+ $self->{'end'} = $hend;
+ $self->{'strand'} = $hstrand;
+ $self->{'species'} = $hspecies;
+
+ $self->{'hstart'} = $hstart;
+ $self->{'hend'} = $hend;
+ $self->{'hstrand'} = $hstrand;
+ $self->{'hseqname'} = ($slice) ? $slice->seq_region_name : '';
+ $self->{'hspecies'} = $species;
+}
- $self->feature2($self->feature1);
- $self->feature1($tmp);
-}
=head2 validate
- Title : validate
- Usage : $sf->validate
- Function: Checks whether all data fields are filled
- in in the object and whether it is of
- the correct type.
- Throws an exception if it finds problems
- Example : $sf->validate
- Returns : nothing
- Args : none
-
+ Description: DEPRECATED do not use
=cut
sub validate {
my ($self) = @_;
-
- $self->SUPER::validate();
- $self->feature2->validate;
- #$f2->validate();
-
- # Now the analysis object
- if (defined($self->analysis)) {
- unless($self->analysis->isa("Bio::EnsEMBL::Analysis")) {
- $self->throw("Wrong type of analysis object");
- }
- } else {
- $self->throw("No analysis object defined");
- }
+
+ deprecate('This method does nothing and should not be used.');
}
=head2 validate_prot_feature
- Title : validate_prot_feature
- Usage :
- Function:
- Example :
- Returns :
- Args :
+ Description: DEPRECATED do not use
=cut
sub validate_prot_feature{
- my ($self) = @_;
+ my ($self) = @_;
- $self->SUPER::validate_prot_feature(1);
- $self->feature2->validate_prot_feature(2);
+ deprecate('This method does nothing and should not be used.');
+}
- if (defined($self->analysis)) {
- unless($self->analysis->isa("Bio::EnsEMBL::Analysis")) {
- $self->throw("Wrong type of analysis object");
- }
- } else {
- $self->throw("No analysis object defined");
- }
- }
=head2 set_featurepair_fields
- Title : set_featurepair_fields
- Usage : $fp->set_featurepair_fields($start, $end, $strand,
- $score, $seqname, $hstart, $hend, $hstrand,
- $hseqname, $analysis);
- Returns : nothing
- Args : listed above, followed by optional $e_value, $perc_id,
- $phase, $end_phase
+ Description: DEPRECATED do not use
=cut
@@ -406,8 +508,11 @@ sub set_featurepair_fields {
my ($self, $start, $end, $strand, $score, $seqname, $hstart, $hend,
$hstrand, $hseqname, $analysis, $e_value, $perc_id,
$phase, $end_phase) = @_;
-
- $self->throw('interface fault') if (@_ < 12 or @_ > 15);
+
+ deprecate("Use individual Getter/Setters or Constructor arguments " .
+ " instead.\nThere is no advantage to using this method.");
+
+ throw('interface fault') if (@_ < 12 or @_ > 15);
$self->start($start);
$self->end($end);
@@ -426,14 +531,29 @@ sub set_featurepair_fields {
}
+=head2 gffstring
+
+ Description: DEPRECATED do not use
+
+=cut
sub gffstring {
my ($self) = @_;
- my $str = $self->SUPER::gffstring();
+ deprecate('Do not use');
+
+ my $str .= (defined $self->slice) ? $self->slice->name()."\t": "\t";
+ $str .= "\t"; #source tag
+ $str .= "\t"; #primary tag
+ $str .= (defined $self->start) ? $self->start."\t" : "\t";
+ $str .= (defined $self->end) ? $self->end."\t" : "\t";
+ $str .= (defined $self->score) ? $self->score."\t" : "\t";
+ $str .= (defined $self->strand) ? $self->strand."\t" : ".\t";
+ $str .= ".\t"; #phase
+ $str .= ".\t"; #end phase
my $hstrand = "+";
-
+
if (($self->hstrand)&&($self->hstrand == -1)) {
$hstrand = "-";
}
@@ -444,7 +564,7 @@ sub gffstring {
$str .= (defined $self->hend) ? $self->hend."\t" : "\t";
$str .= (defined $self->hstrand) ? $hstrand."\t" : "\t";
$str .= (defined $self->hphase) ? $self->hphase."\t" : ".\t";
-
+
return $str;
}
@@ -453,129 +573,41 @@ sub gffstring {
=head2 hphase
- Title : hphase
- Usage : $hphase = $fp->hphase()
- $fp->hphase($hphase)
- Function: get/set on start hphase of predicted feature2
- Returns : [0,1,2]
- Args : none if get, 0,1 or 2 if set.
+ Description: DEPRECATED do not use
=cut
sub hphase {
my ($self, $value) = @_;
-
+
+ deprecate('This method does nothing useful.');
+
if (defined($value)) {
$self->{_hphase} = $value;
}
-
+
return $self->{_hphase};
}
=head2 hend_phase
- Title : hend_phase
- Usage : $hend_phase = $feat->hend_phase()
- $feat->hend_phase($hend_phase)
- Function: get/set on end phase of predicted feature2
- Returns : [0,1,2]
- Args : none if get, 0,1 or 2 if set.
+ Description: DEPRECATED do not use
=cut
sub hend_phase {
my ($self, $value) = @_;
-
+
+ deprecate('This method does nothing useful.');
+
if (defined($value)) {
$self->{_hend_phase} = $value;
}
-
return $self->{_hend_phase};
}
-=head2 species
-
- Arg [1] : string $genus_species_name (optional)
- e.g. Homo_sapiens or Mus_musculus
- Example :
- Description: get/set on the species of feature1
- Returntype : string
- Execeptions: none
- Caller : general
-
-=cut
-
-sub species{
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
- return $self->{'_species'} = $arg;
- }
- return $self->{'_species'};
-}
-
-=head2 hspecies
-
- Arg [1] : string $genus_species_name (optional)
- e.g. Homo_sapiens or Mus_musculus
- Example :
- Description: get/set on the species of feature2
- Returntype : string
- Execeptions: none
- Caller : general
-
-=cut
-
-sub hspecies{
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
- return $self->{'_hspecies'} = $arg;
- }
- return $self->{'_hspecies'};
-}
-
-=head2 identical_matches
-
- Arg [1] : int $identical_matches (optional)
- Example :
- Description: get/set on the number of identical matches
- Returntype : int
- Execeptions: none
- Caller : general
-
-=cut
-
-sub identical_matches{
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
- return $self->{'_identical_matches'} = $arg;
- }
- return $self->{'_identical_matches'};
-}
-
-=head2 positive_matches
-
- Arg [1] : int $positive_matches (optional)
- Example :
- Description: get/set on the number of positive matches
- Returntype : int
- Execeptions: none
- Caller : general
-
-=cut
-
-sub positive_matches{
- my ($self,$arg) = @_;
-
- if (defined($arg)) {
- return $self->{'_positive_matches'} = $arg;
- }
- return $self->{'_positive_matches'};
-}
1;
diff --git a/modules/Bio/EnsEMBL/Gene.pm b/modules/Bio/EnsEMBL/Gene.pm
index e256a9b4a7..262c6bf557 100755
--- a/modules/Bio/EnsEMBL/Gene.pm
+++ b/modules/Bio/EnsEMBL/Gene.pm
@@ -30,17 +30,20 @@ package Bio::EnsEMBL::Gene;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::Root;
-use Bio::EnsEMBL::TranscriptI;
-use Bio::Annotation::DBLink;
-use Bio::EnsEMBL::DBEntry;
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(throw warning deprecate);
-@ISA = qw(Bio::EnsEMBL::Root);
+
+@ISA = qw(Bio::EnsEMBL::Feature);
=head2 new
- Arg [1] : none
+ Arg [start] : int $start
+ Arg [end] : int $end
+ Arg [strand] : 1,-1 $strand
+ Arg [slice] : Bio::EnsEMBL::Slice $slice
Example : $gene = Bio::EnsEMBL::Gene->new();
Description: Creates a new gene object
Returntype : Bio::EnsEMBL::Gene
@@ -50,198 +53,26 @@ use Bio::EnsEMBL::DBEntry;
=cut
sub new {
- my($class,@args) = @_;
-
- my $self = bless {}, $class;
- $self->{'_transcript_array'} = [];
-
- return $self;
-}
-
-
-
-=head2 start
-
- Arg [1] : (optional) int $start
- Example : $start = $gene->start;
- Description: This is a convenience method. It may be better to calculate
- your own start by looking at the exons yourself.
- Gets/sets the lowest start coordinate of this genes exons.
- No consistancy check is performed and if this is used as a
- setter potentially the start could be set to a value which
- does not correspond to the lowest exon start. If this
- gene is in RawContig coordinates and its exons span multiple
- contigs the lowest value is still returned and a warning is
- issued.
- Returntype : int
- Exceptions : warning if gene spans multiple contigs
- Caller : general, contigview
-
-=cut
-
-sub start {
- my($self, $start) = @_;
-
- my $multi_flag = 0;
-
- if($start) {
- $self->{start} = $start;
- } elsif(!defined $self->{start}) {
- my $last_contig;
- foreach my $exon (@{$self->get_all_Exons}) {
- if(!defined($self->{start}) || $exon->start() < $self->{start}) {
- $self->{start} = $exon->start();
- }
- $multi_flag = 1 if($last_contig && $last_contig ne $exon->contig->name);
- $last_contig = $exon->contig->name;
- }
- }
-
- if($multi_flag) {
- use Carp 'cluck';
- cluck("Bio::EnsEMBL::Gene::start - Gene spans multiple contigs.");
- $self->warn("Bio::EnsEMBL::Gene::start - Gene spans multiple contigs." .
- "The return value from start may not be what you want");
- }
-
- return $self->{start};
-}
-
-
-
-=head2 end
-
- Arg [1] : (optional) int $end
- Example : $end = $gene->end;
- Description: This is a convenience method. It may be better to calculate
- your own end by looking at the exons yourself.
- Gets/sets the highest end coordinate of this genes exons.
- No consistancy check is performed and if this is used as a
- setter potentially the end could be set to a value which
- does not correspond to the highest exon end. If this
- gene is in RawContig coordinates and its exons span multiple
- contigs the highest value is still returned and a warning is
- issued.
- Returntype : int
- Exceptions : warning if gene spans multiple contigs
- Caller : general, contigview
-
-=cut
-
-sub end {
- my($self, $end) = @_;
-
- my $multi_flag = 0;
-
- if($end) {
- $self->{end} = $end;
- } elsif(!defined $self->{end}) {
- my $last_contig;
- foreach my $exon (@{$self->get_all_Exons()}) {
- if(!defined($self->{end}) || $exon->end() > $self->{end}) {
- $self->{end} = $exon->end();
- }
- $multi_flag = 1 if($last_contig && $last_contig ne $exon->contig->name);
- $last_contig = $exon->contig->name;
- }
- }
-
- if($multi_flag) {
- cluck("Bio::EnsEMBL::Gene::start - Gene spans multiple contigs.");
- $self->warn("Bio::EnsEMBL::Gene::end - Gene spans multiple contigs." .
- "The return value from end may not be what you want");
- }
-
- return $self->{end};
-}
-
-
-
-=head2 strand
-
- Arg [1] : (optional) int strand
- Example : $strand = $gene->strand;
- Description: This is a convenience method. It may be better just to
- get the strand from this genes exons yourself.
- Gets/Sets the strand of this gene. No consistancy check is
- performed and if used as a setter the strand can be set
- incorrectly. If this gene is in RawContig coords and spans
- multiple contigs it is not possible to calculate the strand
- correctly, and a warning is returned.
- Returntype : int
- Exceptions : Warning if strand is not defined and Gene is in RawContig coords
- so strand cannot be calculated
- Caller : general
-
-=cut
-
-sub strand {
- my $self = shift;
- my $arg = shift;
-
- if( defined $arg ) {
- $self->{'strand'} = $arg;
- } elsif( ! defined $self->{strand} ) {
- my $exons = $self->get_all_Exons();
- if(@$exons) {
- if($exons->[0]->contig &&
- $exons->[0]->contig->isa("Bio::EnsEMBL::RawContig")) {
- my $last_contig;
- foreach my $exon (@$exons) {
- if($last_contig && $last_contig ne $exon->contig->name) {
- $self->warn("Bio::EnsEMBL::Gene::strand - strand can not be " .
- "calculated for a Gene in RawContig coordinates that "
- . "spans multiple contigs");
- return 0;
- }
- $last_contig = $exon->contig->name;
- }
- }
- $self->{'strand'} = $exons->[0]->strand();
- }
- }
- return $self->{'strand'};
+ my $caller = shift;
-}
+ my $class = ref($caller) || $caller;
+ my $self = $class->SUPER::new(@_);
+ my ( $stable_id, $version, $external_name, $type, $external_db,
+ $external_status, $display_xref, $description ) =
+ rearrange( [ 'STABLE_ID', 'VERSION', 'EXTERNAL_NAME', 'TYPE',
+ 'EXTERNAL_DB', 'EXTERNAL_STATUS', 'DISPLAY_XREF', 'DESCRIPTION' ], @_ );
-=head2 chr_name
+ $self->stable_id( $stable_id );
+ $self->version( $version );
+ $self->external_name( $external_name ) if( defined $external_name );
+ $self->external_db( $external_db ) if( defined $external_db );
+ $self->external_status( $external_status ) if( defined $external_status );
+ $self->display_xref( $display_xref ) if( defined $display_xref );
+ $self->type( $type ) if( defined $type );
+ $self->description($description);
- Arg [1] : (optional) string $chr_name
- Example : $chr_name = $gene->chr_name
- Description: Getter/Setter for the name of the chromosome that this
- Gene is on. This is really just a shortcut to the slice
- attached this genes exons, but the value can also be set, which
- is useful for use with the lite database and web code.
- This function will return undef if this gene is not attached
- to a slice and the chr_name attribute has not already been set.
- Returntype : string
- Exceptions : warning if chr_name is not defined and Gene is in RawContig
- coordinates
- Caller : Lite GeneAdaptor, domainview
-
-=cut
-
-sub chr_name {
- my ($self, $chr_name) = @_;
-
- if(defined $chr_name) {
- $self->{'_chr_name'} = $chr_name;
- } elsif(!defined $self->{'_chr_name'}) {
- #attempt to get the chr_name from the contig attached to the exons
- my ($exon, $contig);
- ($exon) = @{$self->get_all_Exons()};
- if($exon && ($contig = $exon->contig())) {
- if(ref $contig && $contig->isa('Bio::EnsEMBL::Slice')) {
- $self->{'_chr_name'} = $contig->chr_name();
- } else {
- $self->warn('Gene::chr_name - Gene is in RawContig coords, and must '
- . 'be in Slice coords to have a valid chr_name');
- }
- }
- }
-
- return $self->{'_chr_name'};
+ return $self;
}
@@ -250,8 +81,7 @@ sub chr_name {
Args : none
Example : none
- Description: returns true if the Gene or one of its Transcripts have
- DBLinks
+ Description: returns true if this gene has a display_xref
Returntype : 0,1
Exceptions : none
Caller : general
@@ -260,94 +90,11 @@ sub chr_name {
sub is_known{
- my ($self) = @_;
-
- for my $entry ( @{$self->get_all_DBLinks()} ) {
- return 1 if uc($entry->status) eq "KNOWN";
- }
-
- foreach my $trans ( @{$self->get_all_Transcripts} ) {
- for my $entry ( @{$trans->get_all_DBLinks()} ) {
- return 1 if uc($entry->status) eq "KNOWN";
- }
- }
-
- return 0;
-}
-
-
-=head2 adaptor
-
- Arg [1] : Bio::EnsEMBL::DBSQL::GeneAdaptor $adaptor
- Example : $gene->adaptor($gene_adaptor);
- $gene->adaptor(undef); # to drop adaptor
- Description: get/set for attribute adaptor
- Returntype : Bio::EnsEMBL::DBSQL::GeneAdaptor
- Exceptions : none
- Caller : set only used by adaptor on store or retrieve
-
-=cut
-
-
-sub adaptor {
- my $self = shift;
-
- if (@_) {
- # Testing for any arguments allows undef to be
- # passed as an argument to unset the adaptor
- $self->{'_adaptor'} = shift;
- }
- return $self->{'_adaptor'};
-}
-
-
-
-=head2 analysis
-
- Arg [1] : Bio::EnsEMBL::Analysis $analysis
- Example : none
- Description: get/set for attribute analysis
- Returntype : Bio::EnsEMBL::Analysis
- Exceptions : none
- Caller : general
-
-=cut
-
-
-sub analysis {
- my ($self,$value) = @_;
- if( defined $value ) {
- $self->{'analysis'} = $value;
- }
- return $self->{'analysis'};
-}
-
-
-
-
-=head2 dbID
-
- Arg [1] : int $dbID
- Example : none
- Description: get/set for attribute dbID
- Returntype : int
- Exceptions : none
- Caller : set only by adaptor on store or retrieve
-
-=cut
-
-
-sub dbID {
- my ($self, $arg) = @_;
-
- if ( defined $arg ) {
- $self->{'_dbID'} = $arg ;
- }
- return $self->{'_dbID'};
+ my $self = shift;
+ return ($self->{'display_xref'}) ? 1 : 0;
}
-
=head2 external_name
Arg [1] : string $external_name
@@ -360,14 +107,12 @@ sub dbID {
=cut
sub external_name {
- my ($self, $ext_name) = @_;
+ my $self = shift;
- if(defined $ext_name) {
- return ( $self->{'_ext_name'} = $ext_name );
- }
+ $self->{'external_name'} = shift if( @_ );
- if( exists $self->{'_ext_name'} ) {
- return $self->{'_ext_name'};
+ if( exists $self->{'external_name'} ) {
+ return $self->{'external_name'};
}
my $display_xref = $self->display_xref();
@@ -393,14 +138,12 @@ sub external_name {
=cut
sub external_db {
- my ( $self, $ext_dbname ) = @_;
+ my $self = shift;
- if(defined $ext_dbname) {
- return ( $self->{'_ext_dbname'} = $ext_dbname );
- }
+ $self->{'external_db'} = shift if( @_ );
- if( exists $self->{'_ext_dbname'} ) {
- return $self->{'_ext_dbname'};
+ if( exists $self->{'external_db'} ) {
+ return $self->{'external_db'};
}
my $display_xref = $self->display_xref();
@@ -445,30 +188,17 @@ sub external_status {
Arg [1] : (optional) string $description
Example : none
- Description: you can set get this argument. If not set its lazy loaded
- from attached adaptor.
+ Description: getter setter for gene description
Returntype : string
- Exceptions : if no GeneAdaptor is set and no description is there
+ Exceptions : none
Caller : general
=cut
-
sub description {
- my ($self, $arg) = @_;
-
- if( defined $arg ) {
- $self->{'_description'} = $arg;
- return $arg;
- }
-
- if( exists $self->{'_description'} ) {
- return $self->{'_description'};
- }
- elsif (my $aptr = $self->adaptor) {
- $self->{'_description'} = $aptr->get_description($self->dbID);
- }
- return $self->{'_description'};
+ my $self = shift;
+ $self->{'description'} = shift if( @_ );
+ return $self->{'description'};
}
@@ -492,7 +222,7 @@ sub add_DBEntry {
my $dbe = shift;
unless($dbe && ref($dbe) && $dbe->isa('Bio::EnsEMBL::DBEntry')) {
- $self->throw('Expected DBEntry argument');
+ throw('Expected DBEntry argument');
}
$self->{'dbentries'} ||= [];
@@ -585,8 +315,8 @@ sub get_all_Exons {
my @out = ();
foreach my $trans ( @{$self->get_all_Transcripts} ) {
- foreach my $exon ( @{$trans->get_all_Exons} ) {
- $h{"$exon"} = $exon;
+ foreach my $e ( @{$trans->get_all_Exons} ) {
+ $h{$e->start()."-".$e->end()."-".$e->strand()."-".$e->phase()} = $e;
}
}
@@ -610,12 +340,11 @@ sub get_all_Exons {
sub type {
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'type'} = $value;
- }
- return $obj->{'type'};
+ my $self = shift;
+
+ $self->{'type'} = shift if( @_ );
+
+ return $self->{'type'};
}
@@ -637,16 +366,31 @@ sub type {
sub add_Transcript{
my ($self,$trans) = @_;
- if( !ref $trans || ! $trans->isa("Bio::EnsEMBL::TranscriptI") ) {
- $self->throw("$trans is not a Bio::EnsEMBL::TranscriptI!");
+ if( !ref $trans || ! $trans->isa("Bio::EnsEMBL::Transcript") ) {
+ throw("$trans is not a Bio::EnsEMBL::Transcript!");
+ }
+
+ if( defined $self->{'start'} ) {
+ if( $self->{'start'} > $trans->start() ) {
+ $self->start( $trans->start() );
+ }
+ } else {
+ $self->start( $trans->start() );
+ }
+
+ if( defined $self->{'end'} ) {
+ if( $self->{'end'} < $trans->end() ) {
+ $self->end( $trans->end() );
+ }
+ } else {
+ $self->end( $trans->end() );
}
- #invalidate the start and end since they may need to be recalculated
- $self->{start} = undef;
- $self->{end} = undef;
- $self->{strand} = undef;
+ $self->{strand} = $trans->strand();;
+ $self->{'_transcript_array'} ||= [];
push(@{$self->{'_transcript_array'}},$trans);
+ $self->recalculate_coordinates();
}
@@ -666,321 +410,351 @@ sub add_Transcript{
sub get_all_Transcripts {
my ($self) = @_;
+ if( ! exists $self->{'_transcript_array'} ) {
+ if( defined $self->adaptor() ) {
+ my $ta = $self->adaptor()->db()->get_TranscriptAdaptor();
+ my $transcripts = $ta->fetch_all_by_Gene( $self );
+ $self->{'_transcript_array'} = $transcripts;
+ }
+ }
return $self->{'_transcript_array'};
}
-=head2 created
+=head2 get_all_alt_alleles
- Arg [1] : string $created
- The time the stable id for this gene was created. Not very well
- maintained data (at release 9)
- Example : none
- Description: get/set/lazy_load for the created timestamp
- Returntype : string
+ Arg [1] : none
+ Example : ( optional )
+ Description: Return a listref of Gene objects that represent this Gene on
+ an alternative haplotype. Empty list if there is no such
+ Gene. (eg there is no overlapping haplotype)
+ Returntype : listref of Bio::EnsEMBL::Gene
Exceptions : none
Caller : general
=cut
+sub get_all_alt_alleles {
+ my $self = shift;
+ my $result = $self->adaptor()->fetch_all_alt_alleles( $self );
+ return $result;
+}
-sub created{
- my ($self,$value) = @_;
-
- if(defined $value ) {
- $self->{'_created'} = $value;
- }
- if( exists $self->{'_created'} ) {
- return $self->{'_created'};
- }
+=head2 version
- $self->_get_stable_entry_info();
+ Arg [1] : int $version
+ A version number for the stable_id
+ Example : nonen
+ Description: getter/setter for version number
+ Returntype : int
+ Exceptions : none
+ Caller : general
- return $self->{'_created'};
+=cut
+sub version{
+ my $self = shift;
+ $self->{'version'} = shift if(@_);
+ return $self->{'version'};
}
-=head2 modified
+=head2 stable_id
- Arg [1] : string $modified
- The time the gene with this stable_id was last modified.
- Not well maintained data (release 9)
- Example : none
- Description: get/set/lazy_load of modified timestamp
+ Arg [1] : string $stable_id
+ Example : ("ENSG0000000001")
+ Description: getter/setter for stable id for this gene
Returntype : string
Exceptions : none
Caller : general
=cut
-
-sub modified{
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_modified'} = $value;
- }
-
- if( exists $self->{'_modified'} ) {
- return $self->{'_modified'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_modified'};
+sub stable_id{
+ my $self = shift;
+ $self->{'stable_id'} = shift if(@_);
+ return $self->{'stable_id'};
}
+=head2 transform
-=head2 version
-
- Arg [1] : int $version
- A version number for the stable_id
- Example : nonen
- Description: get/set/lazy_load for the version number
- Returntype : int
- Exceptions : none
+ Arg 1 : String $coordinate_system_name
+ Arg [2] : String $coordinate_system_version
+ Description: moves this gene to the given coordinate system. If this gene has Transcripts
+ attached, they move as well.
+ Returntype : Bio::EnsEMBL::Gene
+ Exceptions : wrong parameters
Caller : general
=cut
-sub version{
-
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_version'} = $value;
- }
+sub transform {
+ my $self = shift;
- if( exists $self->{'_version'} ) {
- return $self->{'_version'};
- }
- $self->_get_stable_entry_info();
+ # catch for old style transform calls
+ if( !@_ || ( ref $_[0] && $_[0]->isa( "Bio::EnsEMBL::Slice" ))) {
+ deprecate('Calling transform without a coord system name is deprecated.');
+ return $self->_deprecated_transform(@_);
+ }
- return $self->{'_version'};
+ my $new_gene = $self->SUPER::transform( @_ );
+ return undef unless $new_gene;
+ if( exists $self->{'_transcript_array'} ) {
+ my @new_transcripts;
+ for my $old_transcript ( @{$self->{'_transcript_array'}} ) {
+ my $new_transcript = $old_transcript->transform( @_ );
+ push( @new_transcripts, $new_transcript );
+ }
+ $new_gene->{'_transcript_array'} = \@new_transcripts;
+ }
+ return $new_gene;
}
-=head2 stable_id
+=head2 transfer
- Arg [1] : string $stable_id
- Example : ("ENSG0000000001")
- Description: get/set/lazy_loaded stable id for this gene
- Returntype : string
+ Arg [1] : Bio::EnsEMBL::Slice $destination_slice
+ Example : none
+ Description: MOves this Gene to given target slice coordinates. If Transcripts
+ are attached they are moved as well. Returns a new gene.
+ Returntype : Bio::EnsEMBL::Gene
Exceptions : none
Caller : general
=cut
-sub stable_id{
+sub transfer {
+ my $self = shift;
+
+ my $new_gene = $self->SUPER::transfer( @_ );
+ return undef unless $new_gene;
+
+ if( exists $self->{'_transcript_array'} ) {
+ my @new_transcripts;
+ for my $old_transcript ( @{$self->{'_transcript_array'}} ) {
+ my $new_transcript = $old_transcript->transfer( @_ );
+ push( @new_transcripts, $new_transcript );
+ }
+ $new_gene->{'_transcript_array'} = \@new_transcripts;
+ }
+ return $new_gene;
+}
- my ($self,$value) = @_;
-
- if( defined $value ) {
- $self->{'_stable_id'} = $value;
- return;
- }
- if( exists $self->{'_stable_id'} ) {
- return $self->{'_stable_id'};
- }
+=head2 display_xref
- $self->_get_stable_entry_info();
+ Arg [1] : Bio::EnsEMBL::DBEntry $display_xref
+ Example : $gene->display_xref($db_entry);
+ Description: get/set display_xref for this gene
+ Returntype : Bio::EnsEMBL::DBEntry
+ Exceptions : none
+ Caller : general
- return $self->{'_stable_id'};
+=cut
+sub display_xref {
+ my $self = shift;
+ $self->{'display_xref'} = shift if(@_);
+ return $self->{'display_xref'};
}
-
-=head2 _get_stable_entry_info
+=head2 recalculate_coordinates
Args : none
Example : none
- Description: does the lazy loading for all stable id related information
+ Description: called when transcript added to the gene
+ tries to set coords for the gene.
Returntype : none
Exceptions : none
Caller : internal
=cut
+sub recalculate_coordinates {
+ my $self = shift;
-sub _get_stable_entry_info {
- my $self = shift;
-
- if( !defined $self->adaptor ) {
- return undef;
- }
+ my $transcripts = $self->get_all_Transcripts();
- $self->adaptor->get_stable_entry_info($self);
+ return if(!$transcripts || !@$transcripts);
-}
+ my ( $slice, $start, $end, $strand );
+ $slice = $transcripts->[0]->slice();
+ $strand = $transcripts->[0]->strand();
+ $start = $transcripts->[0]->start();
+ $end = $transcripts->[0]->end();
+ my $transsplicing = 0;
+ for my $t ( @$transcripts ) {
+ if( $t->start() < $start ) {
+ $start = $t->start();
+ }
-sub _dump{
- my ($self,$fh) = @_;
+ if( $t->end() < $end ) {
+ $end = $t->end();
+ }
- if( ! $fh ) {
- $fh = \*STDOUT;
- }
+ if( $t->slice()->name() ne $slice->name() ) {
+ throw( "Transcripts with different slices not allowed on one Gene" );
+ }
- print $fh "Gene ", $self->dbID(), "\n";
- foreach my $t ( @{$self->get_all_Transcripts()} ) {
- print $fh " Trans ", $t->dbID(), " :";
- foreach my $e ( @{$t->get_all_Exons} ) {
- print $fh " ",$e->dbID(),",";
- }
- print "\n";
- }
+ if( $t->strand() != $strand ) {
+ $transsplicing = 1;
+ }
+ }
+ if( $transsplicing ) {
+ warning( "Gene contained trans splicing event" );
+ }
+ $self->start( $start );
+ $self->end( $end );
+ $self->strand( $strand );
+ $self->slice( $slice );
}
-=head2 transform
- Arg 1 : (optional) Bio::EnsEMBL::Slice $slice
- Description: when passed a Slice as argument,
- it will transform this Gene to the Slice coordinate system.
- Without an argument it transforms the Gene (which should be
- in a slice) to a RawContig
- coordinate system.
- The method changes the Gene in place and returns itself.
- Returntype : Bio::EnsEMBL::Gene
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $gene->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For genes this is the stable id if
+ it is available otherwise it is an empty string.
+ Returntype : string
Exceptions : none
- Caller : object::methodname or just methodname
+ Caller : web drawing code
=cut
-
-sub transform {
+sub display_id {
my $self = shift;
- my $slice = shift;
+ return $self->{'stable_id'} || '';
+}
- # hash arrray to store the refs of transformed exons
- my %exon_transforms;
- # transform Exons
- for my $exon ( @{$self->get_all_Exons()} ) {
- my $newExon = $exon->transform( $slice );
- $exon_transforms{ $exon } = $newExon;
- }
- # now need to re-jiggle the transcripts and their
- # translations to account for the re-mapping process
+###########################
+# DEPRECATED METHODS FOLLOW
+###########################
- foreach my $transcript ( @{$self->get_all_Transcripts()} ) {
+=head2 DEPRECATED add_DBLink
- # need to grab the translation before starting to
- # re-jiggle the exons
+ Description: DEPRECATED This method has been deprecated in favor of the
+ add_DBEntry method. Objects are responible for holding only
+ xrefs directly associated with themselves now.
- $transcript->transform( \%exon_transforms );
- }
+=cut
- #unset the start, end, and strand - they need to be recalculated
- $self->{start} = undef;
- $self->{end} = undef;
- $self->{strand} = undef;
- $self->{_chr_name} = undef;
- return $self;
+sub add_DBLink{
+ my ($self,$value) = @_;
+
+ throw("add_DBLink is deprecated. You probably want add_DBEntry.");
+
+# unless(defined $value && ref $value
+# && $value->isa('Bio::Annotation::DBLink') ) {
+# throw("This [$value] is not a DBLink");
+# }
+
+# if( !defined $self->{'_db_link'} ) {
+# $self->{'_db_link'} = [];
+# }
+
+# push(@{$self->{'_db_link'}},$value);
}
-=head2 temporary_id
- Title : temporary_id
- Usage : $obj->temporary_id($newval)
- Function: Temporary ids are used for Genscan predictions - which should probably
- be moved over to being stored inside the gene tables anyway. Bio::EnsEMBL::TranscriptFactory use this.
- MC Over my dead body they will. Unless you can speed up the database by a couple of orders of magnitude.
- Example :
- Returns : value of temporary_id
- Args : newvalue (optional)
+=head2 temporary_id
+ Function: DEPRECATED: Use dbID or stable_id or something else instead
=cut
sub temporary_id {
my ($obj,$value) = @_;
+ deprecate( "I cant see what a temporary_id is good for, please use " .
+ "dbID or stableID or\n try without an id." );
if( defined $value) {
$obj->{'temporary_id'} = $value;
}
return $obj->{'temporary_id'};
-
}
+=head2 created
-=head2 display_xref
-
- Arg [1] : Bio::EnsEMBL::DBEntry $display_xref
- Example : $gene->display_xref($db_entry);
- Description: get/set/lazy_loaded display_xref for this gene
- Returntype : Bio::EnsEMBL::DBEntry
- Exceptions : none
- Caller : general
+ Description: DEPRECATED - Transcripts no longer have a created attribute
=cut
-sub display_xref {
-
- my $self = shift;
- if( @_ ) {
- $self->{'display_xref'} = shift;
- } elsif( exists $self->{'display_xref'} ) {
- return $self->{'display_xref'};
- } elsif ( defined $self->adaptor() ) {
- $self->{'display_xref'} = $self->adaptor->get_display_xref( $self );
+sub created{
+ my ($self,$value) = @_;
+ deprecate( "The created attribute isnt available any more" );
+ if(defined $value ) {
+ $self->{'created'} = $value;
}
-
- return $self->{'display_xref'};
+ return $self->{'created'};
}
+=head2 modified
+ Description: DEPRECATED - Transcripts no longer have a modified attribute
-=head2 DEPRECATED add_DBLink
+=cut
- Arg [1] : DEPRECATED Bio::Annotation::DBLink $link
- a link is a database entry somewhere else.
- Usually this is a Bio::EnsEMBL::DBEntry.
- Example : DEPRECATED
- Description: This method has been deprecated in favor of the add_DBEntry
- method. Objects are responible for holding only xrefs directly
- associated with themselves now.
- Returntype : none
- Exceptions : none
- Caller : general
+sub modified {
+ my ($self,$value) = @_;
+ deprecate( "The modified item isnt available any more" );
+ if( defined $value ) {
+ $self->{'modified'} = $value;
+ }
+ return $self->{'modified'};
+}
+
+=head2 chr_name
+
+ Description: DEPRECATED. Use project, tranform, or transfer to obtain this
+ gene in another coord system. Use $gene->slice->seq_region_name
+ to get the name of the underlying coord system. Or
+ $gene->slice->name().
=cut
+sub chr_name {
+ my $self = shift;
-sub add_DBLink{
- my ($self,$value) = @_;
+ deprecate( "Use project() to obtain other coordinate systems" );
- $self->throw("add_DBLink is deprecated. You probably want add_DBEntry.");
+ my $gene_slice = $self->slice();
+ if( $gene_slice->coord_system()->name eq "chromosome" ) {
+ return $gene_slice->seq_region_name();
+ }
-# unless(defined $value && ref $value
-# && $value->isa('Bio::Annotation::DBLink') ) {
-# $self->throw("This [$value] is not a DBLink");
-# }
-
-# if( !defined $self->{'_db_link'} ) {
-# $self->{'_db_link'} = [];
-# }
+ my $sa = $self->slice->adaptor();
+ throw( "need db connection for chr_name call" ) unless $sa;
-# push(@{$self->{'_db_link'}},$value);
+ my $ca = $sa->db()->get_CoordSystemAdaptor();
+ my $coord_system = $ca->fetch_by_name( "chromosome" );
+ if( ! $coord_system ) {
+ throw( "Chromosome coordinate system not available" );
+ }
+ my $coords = $self->project( $coord_system );
+
+ if( @$coords ) {
+ return $coords->[0]->[2]->seq_region_name();
+ }
}
1;
diff --git a/modules/Bio/EnsEMBL/IdentityXref.pm b/modules/Bio/EnsEMBL/IdentityXref.pm
index 9c5874d1be..3fd74954ee 100644
--- a/modules/Bio/EnsEMBL/IdentityXref.pm
+++ b/modules/Bio/EnsEMBL/IdentityXref.pm
@@ -46,24 +46,10 @@ use strict;
sub new {
my ($class, @args) = @_;
my $self = $class->SUPER::new(@args);
- my ($query_identity, $target_identity, $score, $evalue,
- $cigar_line, $query_start, $query_end, $translation_start,
- $translation_end, $analysis ) = $self->_rearrange(
- [qw(QUERY_IDENTITY TARGET_IDENTITY SCORE EVALUE CIGAR_LINE
- QUERY_START QUERY_END TRANSLATION_START TRANSLATION_END
- ANALYSIS)], @args);
-
- $self->{'query_identity'} = $query_identity;
- $self->{'target_identity'} = $target_identity;
- $self->{'score'} = $score;
- $self->{'evalue'} = $evalue;
- $self->{'cigar_line'} = $cigar_line;
- $self->{'query_start'} = $query_start;
- $self->{'query_end'} = $query_end;
- $self->{'translation_start'} = $translation_start;
- $self->{'translation_end'} = $translation_end;
- $self->{'analysis'} = $analysis;
-
+ my ($query_identity, $target_identity) = $self->_rearrange(
+ [qw(QUERY_IDENTITY TARGET_IDENTITY)], @args);
+ if(defined $query_identity) { $self->query_identity($query_identity); }
+ if(defined $target_identity) { $self->target_identity($target_identity); }
return $self;
}
@@ -110,294 +96,4 @@ sub target_identity{
}
-
-
-=head2 cigar_line
-
- Arg [1] : string $cigar_line
- Example : none
- Description: get/set for attribute cigar_line
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub cigar_line {
- my $self = shift;
- $self->{'cigar_line'} = shift if( @_ );
- return $self->{'cigar_line'};
-}
-
-
-=head2 translation_start
-
- Arg [1] : string $translation_start
- Example : none
- Description: get/set for attribute translation_start
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub translation_start {
- my $self = shift;
- $self->{'translation_start'} = shift if( @_ );
- return $self->{'translation_start'};
-}
-
-
-=head2 translation_end
-
- Arg [1] : string $translation_end
- Example : none
- Description: get/set for attribute translation_end
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub translation_end {
- my $self = shift;
- $self->{'translation_end'} = shift if( @_ );
- return $self->{'translation_end'};
-}
-
-
-=head2 query_start
-
- Arg [1] : string $query_start
- Example : none
- Description: get/set for attribute query_start
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub query_start {
- my $self = shift;
- $self->{'query_start'} = shift if( @_ );
- return $self->{'query_start'};
-}
-
-
-=head2 query_end
-
- Arg [1] : string $query_end
- Example : none
- Description: get/set for attribute query_end
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub query_end {
- my $self = shift;
- $self->{'query_end'} = shift if( @_ );
- return $self->{'query_end'};
-}
-
-
-=head2 score
-
- Arg [1] : string $score
- Example : none
- Description: get/set for attribute score
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub score {
- my $self = shift;
- $self->{'score'} = shift if( @_ );
- return $self->{'score'};
-}
-
-
-=head2 evalue
-
- Arg [1] : string $evalue
- Example : none
- Description: get/set for attribute evalue
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub evalue {
- my $self = shift;
- $self->{'evalue'} = shift if( @_ );
- return $self->{'evalue'};
-}
-
-
-=head2 analysis
-
- Arg [1] : Bio::EnsEMBL::Analysis $analysis
- Example : none
- Description: get/set for attribute analysis
- Returntype : Bio::EnsEMBL::Analysis
- Exceptions : none
- Caller : general
-
-=cut
-
-sub analysis {
- my $self = shift;
- $self->{'analysis'} = shift if( @_ );
- return $self->{'analysis'};
-}
-
-
-
-
-=head2 get_mapper
-
- Args : none
- Example : none
- Description: produces a mapper object that takes coordinates from one side of
- the alignment to the other side. "ensembl" and "external" are the
- two coordinate systems contained.
- Returntype : Bio::EnsEMBL::Mapper
- Exceptions : none
- Caller : general, ProteinDAS subsystem
-
-=cut
-
-
-sub get_mapper {
- my ( $self ) = @_;
- # parse the cigar_line and create a mapper ...
- if( exists $self->{'_cached_mapper'} ) {
- return $self->{'_cached_mapper'};
- }
-
- my ( @lens, @chars );
-
- # if there is no cigar line, nothing is going to be loaded
- if( $self->cigar_line() ) {
- my @pre_lens = split( '[DMI]', $self->cigar_line() );
- @lens = map { if( ! $_ ) { 1 } else { $_ }} @pre_lens;
- @chars = grep { /[DMI]/ } split( //, $self->cigar_line() );
- }
- my $translation_start = $self->translation_start();
- my $translation_end = $self->translation_end();
- my $query_start = $self->query_start();
- my $query_end = $self->query_end();
-
- # my $hit_id = $self->display_id();
- my $hit_id = "external_id";
- my $translation_id = "translation_id";
- # now build the mapper
- my $mapper = Bio::EnsEMBL::Mapper->new( "ensembl", "external" );
-
-
- for( my $i=0; $i<=$#lens; $i++ ) {
- my $length = $lens[$i];
- my $char = $chars[$i];
- if( $char eq "M" ) {
- $mapper->add_map_coordinates( $translation_id, $translation_start,
- $translation_start + $length - 1,
- 1, $hit_id, $query_start, $query_start + $length - 1 );
- $query_start += $length;
- $translation_start += $length;
-
- } elsif( $char eq "D" ) {
- $query_start += $length;
- } elsif( $char eq "I" ) {
- $translation_start += $length;
- }
- }
-
- $self->{'_cached_mapper'} = $mapper;
-
- return $mapper;
-}
-
-
-
-=head2 transform_feature
-
- Arg [1] : a feature type with start and end $feature
- This doesnt need to be a Bio::EnsEMBL::Feature as it doesnt
- need an attached slice. We may have to introduce an appropriate
- object type.
- Example : my $ens_prot_feature_list = $ident_xref->
- transform_feature( $swiss_prot_feature );
- Description: a list of potential partial features which represent all mappable places
- of the original feature in ensembl translation coordinates.
- Returntype : listref of whatever was put in
- Exceptions : none
- Caller : general, ProteinDAS subsystem
-
-=cut
-
-
-sub transform_feature {
- my $self= shift;
- my $feature = shift;
-
- my $fstart = $feature->start();
- my $fend = $feature->end();
-
- my $mapper = $self->get_mapper();
- my @result;
-
- my @coords = $mapper->map_coordinates( "external_id", $fstart, $fend, 1, "external" );
-
- for my $coord ( @coords ) {
- if( $coord->isa( "Bio::EnsEMBL::Mapper::Coordinate" )) {
- my $new_feature;
- %{$new_feature} = %$feature;
- bless $new_feature, ref( $feature );
- $new_feature->start( $coord->start() );
- $new_feature->end( $coord->end() );
-
- push( @result, $new_feature );
- }
- }
-
- return \@result;
-}
-
-
-
-=head2 map_feature
-
- Arg [1] : a start,end capable feature object
- Example : none
- Description:
- Returntype : list of Coordinates/Gaps which represents the mapping
- Exceptions : none
- Caller : another way of doing ProteinDAS
-
-=cut
-
-sub map_feature {
- my $self = shift;
- my $feature = shift;
-
-
- my $fstart = $feature->start();
- my $fend = $feature->end();
-
- my $mapper = $self->get_mapper();
- my @coords = $mapper->map_coordinates( "external_id", $fstart, $fend, 1, "external" );
-
- return @coords;
-}
-
-
-
-
-
-
-
1;
diff --git a/modules/Bio/EnsEMBL/KaryotypeBand.pm b/modules/Bio/EnsEMBL/KaryotypeBand.pm
index 8d9954f89d..3811182a95 100644
--- a/modules/Bio/EnsEMBL/KaryotypeBand.pm
+++ b/modules/Bio/EnsEMBL/KaryotypeBand.pm
@@ -1,7 +1,6 @@
#
# Ensembl module for Bio::EnsEMBL::DBSQL::KaryotypeBand
#
-# Cared for by James Stalker <jws@sanger.ac.uk>
#
# Copyright James Stalker
#
@@ -15,19 +14,33 @@ Bio::EnsEMBL::DBSQL::KaryotypeBand
use Bio::EnsEMBL::KaryotypeBand;
-# create and populate a karyotype band
-$kb = Bio::EnsEMBL::KaryotyeBand;
-$kb->name('q31');
-$kb->chr_name('1');
-$kb->start(1);
-$kb->end(1_000_000);
-$kb->stain('gpos50');
+# create and populate a karyotype band (normally done by adaptor)
+$kb = Bio::EnsEMBL::KaryotyeBand(-START => 1,
+ -END => 1_000_000,
+ -SLICE => $chrX_slice,
+ -NAME => 'q31',
+ -STAIN => 'gpos50',
+ -ADAPTOR => $db->get_KaryotypeBandAdaptor,
+ -DBID => 10);
+
+#can tranform this band into other coord systems, just like other features
+$kb = $kb->transform('supercontig');
+
+$start = $kb->start();
+$end = $kb->end();
+$seq_region = $kb->slice->seq_region_name();
+
+#karyotypes have internal ids as well
+$kary_id = $kb->dbID();
=head1 DESCRIPTION
KaryotypeBand objects encapsulate data pertaining to a single karyotype band.
-Access these objects through a Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor
+Access these objects through a Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor.
+
+KarytoypeBand inherits from Bio::EnsEMBL::Feature and can be used just as
+any other feature can be.
=head1 AUTHOR
@@ -37,7 +50,7 @@ This modules is part of the Ensembl project http://www.ensembl.org
=head1 CONTACT
-Post questions to the EnsEMBL developer mailing list <ensembl-dev@ebi.ac.uk>
+Post questions to the EnsEMBL developer mailing list <ensembl-dev@ebi.ac.uk>
=cut
@@ -45,16 +58,30 @@ package Bio::EnsEMBL::KaryotypeBand;
use strict;
use vars qw(@ISA);
-use Bio::EnsEMBL::Root;
-@ISA = qw(Bio::EnsEMBL::Root);
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(deprecate warning);
+
+@ISA = qw(Bio::EnsEMBL::Feature);
=head2 new
- Arg [1] : none
- Example : $kb = Bio::EnsEMBL::KaryotypeBand->new;
- Description: Constructor. Creates a new KaryotypeBand object
+ Arg [NAME] : string (optional)
+ The name of this band
+ Arg [STAIN]: string (optional)
+ The stain of this band
+ Arg [...] : Arguments passed to superclass constructor.
+ See Bio::EnsEMBL::Feature
+ Example : $kb = Bio::EnsEMBL::KaryotypeBand->new(-START => $start,
+ -END => $end,
+ -SLICE => $slice,
+ -NAME => 'q11.21',
+ -STAIN => 'gneg');
+ Description: Constructor. Creates a new KaryotypeBand object, which can be
+ treated as any other feature object. Note that karyotypes
+ bands always have strand = 0.
Returntype : Bio::EnsEMBL::KarytotypeBand
Exceptions : none
Caller : Bio::EnsEMBL::KaryotypeBandAdaptor
@@ -62,14 +89,17 @@ use Bio::EnsEMBL::Root;
=cut
sub new {
- my ($class) = @_;
+ my $class = shift;
- my $self = {};
- bless $self,$class;
+ my $self = $class->SUPER::new(@_);
- return $self;
-}
+ my ($name, $stain) = rearrange(['NAME','STAIN'],@_);
+ $self->{'name'} = $name;
+ $self->{'stain'} = $stain;
+ $self->{'strand'} = 0;
+ return $self;
+}
=head2 name
@@ -84,101 +114,107 @@ sub new {
=cut
sub name{
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'name'} = $value;
- }
- return $self->{'name'};
+ my $self = shift;
+ $self->{'name'} = shift if(@_);
+ return $self->{'name'};
}
-=head2 chr_name
+=head2 stain
- Arg [1] : (optional) string $chr_name
- Name of the chromosome this Karyotype band is on
- Example : $chr_name = $band->chr_name;
- Description: Getter/Setter for the name of the chromosome this band is on
+ Arg [1] : (optional) string $value
+ Example : my $band_stain = $band->stain();
+ Description: get/set for the band stain (e.g. 'gpos50')
Returntype : string
- Name of the chromosom this band is on
Exceptions : none
Caller : general
=cut
-sub chr_name {
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'_chr_name'} = $value;
- }
- return $self->{'_chr_name'};
+sub stain{
+ my $self = shift;
+ $self->{'stain'} = shift if(@_);
+ return $self->{'stain'};
}
-=head2 start
- Arg [1] : (optional) int $newvalue
- Example : my $band_start = $band->start();
- Description: get/set for the band start (e.g. 10000) in absolute basepairs
- Returntype : int
+=head2 strand
+
+ Arg [1] : none
+ Example : $strand = $qtl_feat->strand();
+ Description: Overrides the Feature strand method to always return a
+ value of 0 for qtl features (they are unstranded features)
+ Returntype : int (always 0)
Exceptions : none
Caller : general
=cut
-sub start{
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'start'} = $value;
- }
- return $self->{'start'};
+sub strand {
+ my $self = shift;
+ return 0;
}
+=head2 move
-=head2 end
-
- Arg [1] : (optional) int $value
- Example : $band_end = $band->end;
- Description: get/set for the band end (e.g. 10000) in absolute basepairs
- Returntype : int
+ Arg [1] : $start - The new end of this band
+ Arg [2] : $end - The new start of this band
+ Arg [3] : $strand - ignored always set to 0
+ Example : $kb->move(1, 10_000);
+ Description: Overrides superclass move() method to ensure strand is always 0.
+ See Bio::EnsEMBL::Feature::move
+ Returntype : none
Exceptions : none
Caller : general
=cut
-sub end{
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'end'} = $value;
- }
- return $self->{'end'};
+sub move {
+ my ($self, $start, $end, $strand) = @_;
+
+ #maintain a strandedness of 0
+ return $self->SUPER::move($start,$end,0);
}
+=head2 display_id
-=head2 stain
-
- Arg [1] : (optional) string $value
- Example : my $band_stain = $band->stain();
- Description: get/set for the band stain (e.g. 'gpos50')
- Returntype : string
+ Arg [1] : none
+ Example : print $kb->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For karyotype bands this is the
+ name of the karyotype band or '' if no name is defined.
+ Returntype : string
Exceptions : none
- Caller : general
+ Caller : web drawing code
=cut
-sub stain{
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'stain'} = $value;
- }
- return $self->{'stain'};
+sub display_id {
+ my $self = shift;
+ return $self->{'name'} || '';
+}
+
+
+=head2 chr_name
+
+ Description: DEPRECATED - use $kary_band->slice()->seq_region_name() instead
+
+=cut
+
+sub chr_name {
+ my $self = shift;
+
+ deprecate('Use $kary_band->slice()->seq_region_name() instead.');
+ if(!$self->slice) {
+ warning('KaryotypeBand does not have Slice - cannot get seq_region_name.');
+ return '';
+ }
+
+ return $self->slice->seq_region_name();
}
diff --git a/modules/Bio/EnsEMBL/Lite/DensityPlot/DensityAdaptor.pm b/modules/Bio/EnsEMBL/Lite/DensityPlot/DensityAdaptor.pm
index 5c8c118b96..0fd7af58e3 100644
--- a/modules/Bio/EnsEMBL/Lite/DensityPlot/DensityAdaptor.pm
+++ b/modules/Bio/EnsEMBL/Lite/DensityPlot/DensityAdaptor.pm
@@ -12,9 +12,10 @@ DensityAdaptor
=head1 SYNOPSIS
my $obj= Bio::EnsEMBL::DBSQL::Obj->new(-dbname=>'ens08',-user=>'ensadmin',-host=>'ensrv5');
+
my $da= Bio::EnsEMBL::DBSQL::DensityAdaptor->new($obj);
+
my $binvalueset = $da->get_density_per_chromosome_type('1','gene');
-my $max_density = $da->get_max_density_per_chromosome('1');
=head1 DESCRIPTION
@@ -48,10 +49,14 @@ use Bio::EnsEMBL::DensityPlot::BinValueSet;
=cut
-sub get_density_per_chromosome_type {
- my ($self,$chromosome,$type) = @_;
+sub get_density_per_chromosome_type
+{
+
+ my ($self,$chromosome,$type)=@_;
+
$self->throw("I need a chromosome") unless defined $chromosome;
$self->throw("I need a type") unless defined $type;
+
my $chr_adaptor = $self->db->get_ChromosomeAdaptor();
my $chr_id = $chr_adaptor->fetch_by_chr_name($chromosome)->dbID();
@@ -67,6 +72,7 @@ sub get_density_per_chromosome_type {
my $res = $sth->execute();
my ($chr_start,$chr_end,$value);
+
$sth->bind_columns(undef,\$chr_start,\$chr_end,\$value);
my $valueset = new Bio::EnsEMBL::DensityPlot::BinValueSet;
@@ -81,45 +87,4 @@ sub get_density_per_chromosome_type {
return $valueset;
}
-=head2 get_max_density_per_chromosome
-
- Arg [1] : string $chr
- the chromomsome to get the max. density from
- Arg [2] : arrayref $ignore_types
- types in map_density to ignore in max. count
- Example : my $max_density = $densityAdaptor->get_max_density_per_chromosome('1');
- Description: Get the max. density from a chromosome
- Returntype : int
- Exceptions : thrown if no chromosome is passed or if no density data is
- returned from db
- Caller : general
-
-=cut
-
-sub get_max_density_per_chromosome {
- my ($self, $chr, $ignore_types) = @_;
- $self->throw("I need a chromosome") unless defined $chr;
- unless (defined($self->{'_max_density'})) {
- $self->{'_max_density'} = {};
- }
- unless ($self->{'_max_density'}->{$chr}) {
- my $chr_adaptor = $self->db->get_ChromosomeAdaptor();
- my $chr_id = $chr_adaptor->fetch_by_chr_name($chr)->dbID();
-
- my $query = "SELECT MAX(value)
- FROM map_density
- WHERE chromosome_id = '$chr_id'";
- if ($ignore_types) {
- my $ignore_str = join("', '", @{$ignore_types});
- $query .= " AND type NOT IN ('" . $ignore_str . "')";
- }
- my $sth = $self->db->prepare($query);
- $sth->execute();
- ($self->{'_max_density'}->{$chr}) = $sth->fetchrow_array()
- or $self->throw("No density data available for chromosome $chr");
- }
- return $self->{'_max_density'}->{$chr};
-}
-
1;
-
diff --git a/modules/Bio/EnsEMBL/Lite/GeneAdaptor.pm b/modules/Bio/EnsEMBL/Lite/GeneAdaptor.pm
index 2729ff32c0..e818a68668 100644
--- a/modules/Bio/EnsEMBL/Lite/GeneAdaptor.pm
+++ b/modules/Bio/EnsEMBL/Lite/GeneAdaptor.pm
@@ -71,6 +71,8 @@ sub new {
return $self;
}
+
+
=head2 fetch_all_by_gene_id_list
Arg [1] : arrayref $gene_ids
@@ -167,7 +169,7 @@ sub fetch_by_gene_id_list {
sub fetch_all_by_Slice {
my ( $self, $slice, $logic_name, $empty_flag ) = @_;
- my $key = $slice->name . (defined ($logic_name) ? ":$logic_name" : "");
+ my $key = $slice->name . ":$logic_name";
if($empty_flag) {
# return from cache or the _get_empty_Genes fn while caching results....
@@ -244,7 +246,6 @@ sub fetch_by_DBEntry {
sub fetch_by_stable_id {
my ($self, $stable_id, $chr_coords) = @_;
- #warn( "~~~~~~~~~~~~~~" );
my $core_db_adaptor = $self->db->get_db_adaptor('core');
my $sth = $self->prepare
diff --git a/modules/Bio/EnsEMBL/Lite/SNPAdaptor.pm b/modules/Bio/EnsEMBL/Lite/SNPAdaptor.pm
index ec68696b67..168fb3cb45 100644
--- a/modules/Bio/EnsEMBL/Lite/SNPAdaptor.pm
+++ b/modules/Bio/EnsEMBL/Lite/SNPAdaptor.pm
@@ -84,9 +84,6 @@ sub fetch_all_by_Slice {
}
#create a snp object through a fast (hacky) constructor
- my $STATUS = $arrayref->[6];
- $STATUS =~s/-/ /;
- $STATUS = $STATUS eq 'no info' ? 'suspected' : "proven $STATUS";
my $snp = Bio::EnsEMBL::SNP->new_fast(
{ 'dbID' => $arrayref->[0],
'_gsf_start' => $arrayref->[1] - $slice_start + 1,
@@ -96,12 +93,10 @@ sub fetch_all_by_Slice {
'_type' => $arrayref->[4],
'_range_type' => $arrayref->[5],
'_validated' => $arrayref->[6],
- 'status' => $STATUS,
'alleles' => $arrayref->[7],
'_ambiguity_code' => $arrayref->[10],
'_snpclass' => $arrayref->[8],
'_mapweight' => $arrayref->[9],
- '_source' => $arrayref->[11],
'_source_tag' => $arrayref->[11],
'link' => \@links });
push @snps, $snp;
diff --git a/modules/Bio/EnsEMBL/Map/DBSQL/MarkerAdaptor.pm b/modules/Bio/EnsEMBL/Map/DBSQL/MarkerAdaptor.pm
index 345ce5e43d..0420a13bd8 100644
--- a/modules/Bio/EnsEMBL/Map/DBSQL/MarkerAdaptor.pm
+++ b/modules/Bio/EnsEMBL/Map/DBSQL/MarkerAdaptor.pm
@@ -65,8 +65,8 @@ sub fetch_all {
my( $marker_id, $priority, $left_primer, $right_primer, $type,
$min_pdist, $max_pdist, $ms_id, $ms_name, $ms_src);
- $sth->bind_columns(\$marker_id, \$priority, \$left_primer,
- \$right_primer, \$type, \$min_pdist, \$max_pdist,
+ $sth->bind_columns(\$marker_id, \$priority,
+ \$left_primer, \$right_primer, \$type, \$min_pdist, \$max_pdist,
\$ms_id, \$ms_name, \$ms_src);
my @out;
@@ -243,17 +243,17 @@ sub fetch_attributes {
while($sth->fetch) {
push @syns, Bio::EnsEMBL::Map::MarkerSynonym->new($ms_id,$ms_src,$ms_name);
- }
+ }
$sth->finish;
$marker->add_MarkerSynonyms(@syns) if(@syns);
-
+
#
# Now retrieve map locations for this marker
#
$marker->flush_MapLocations;
-
- $sth = $self->prepare("SELECT mloc.chromosome_id, mloc.position,
+
+ $sth = $self->prepare("SELECT mloc.chromosome_name, mloc.position,
mloc.lod_score, map.map_name, ms.name
FROM marker_map_location mloc, map map,
marker_synonym ms
@@ -261,24 +261,16 @@ sub fetch_attributes {
AND map.map_id = mloc.map_id
AND ms.marker_synonym_id = mloc.marker_synonym_id");
- my($chr_id, $pos, $lod, $mname, $name);
+ my($chr_name, $pos, $lod, $mname, $name);
my @mlocs;
$sth->execute($m_id);
- $sth->bind_columns(\$chr_id, \$pos, \$lod, \$mname, \$name);
+ $sth->bind_columns(\$chr_name, \$pos, \$lod, \$mname, \$name);
- my %chr_cache;
- my $chr_adaptor = $self->db->get_ChromosomeAdaptor;
while($sth->fetch) {
- my $chr;
- unless($chr = $chr_cache{$chr_id}) {
- $chr = $chr_adaptor->fetch_by_dbID($chr_id);
- $chr_cache{$chr_id} = $chr;
- }
-
- push(@mlocs,
- Bio::EnsEMBL::Map::MapLocation->new($name,$mname, $chr,$pos,$lod));
+ push(@mlocs, Bio::EnsEMBL::Map::MapLocation->new($name, $mname,
+ $chr_name,$pos,$lod));
}
$sth->finish;
diff --git a/modules/Bio/EnsEMBL/Map/DBSQL/MarkerFeatureAdaptor.pm b/modules/Bio/EnsEMBL/Map/DBSQL/MarkerFeatureAdaptor.pm
index 9636ac5970..2714aa24a6 100644
--- a/modules/Bio/EnsEMBL/Map/DBSQL/MarkerFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/Map/DBSQL/MarkerFeatureAdaptor.pm
@@ -34,6 +34,8 @@ use Bio::EnsEMBL::Map::Marker;
use Bio::EnsEMBL::Map::MarkerSynonym;
use Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
+
use vars qw(@ISA);
@ISA = qw(Bio::EnsEMBL::DBSQL::BaseFeatureAdaptor);
@@ -54,9 +56,9 @@ use vars qw(@ISA);
sub fetch_all_by_Marker {
my $self = shift;
my $marker = shift;
-
+
my $constraint = 'm.marker_id = ' . $marker->dbID;
-
+
return $self->generic_fetch($constraint, @_);
}
@@ -100,69 +102,48 @@ sub fetch_all_by_Slice_and_priority {
=head2 fetch_all_by_RawContig_and_priority
- Arg [1] : Bio::EnsEMBL::RawContig $contig
- Arg [2] : (optional) int $priority
- Arg [3] : (optional) string $logic_name
- Example : @feats = @{$mfa->fetch_all_by_RawContig_and_priority($ctg, 80)};
- Description: Retrieves all marker features above a specified threshold
- priority which overlap the provided slice.
- Returntype : listref of Bio::EnsEMBL::Map::MarkerFeatures in contig coords
- Exceptions : none
- Caller : general
+ Description: DEPRECATED use fetch_all_by_Slice_and_priority instead
=cut
sub fetch_all_by_RawContig_and_priority {
- my ($self, $contig, $priority, @args) = @_;
-
- my $constraint;
- if(defined $priority) {
- $constraint = "m.priority > $priority";
- }
-
- return $self->fetch_all_by_RawContig_constraint($contig, $constraint, @args);
+ deprecate('Use fetch_all_by_Slice_and_priority() instead.');
+ fetch_all_by_Slice_and_priority(@_);
}
-
-sub fetch_all_by_RawContig_and_score {
- my $self = shift;
- $self->throw('fetch_all_by_RawContig_and_score should not be used to fetch' .
- ' marker features');
-}
-
sub fetch_all_by_Slice_and_score {
my $self = shift;
- $self->throw('fetch_all_by_Slice_and_score should not be used to fetch' .
- ' marker_features');
+ throw('fetch_all_by_Slice_and_score should not be' .
+ 'used to fetch marker_features');
}
sub _columns {
my $self = shift;
- return ('mf.marker_feature_id', 'mf.marker_id',
- 'mf.contig_id', 'mf.contig_start', 'mf.contig_end',
- 'mf.analysis_id', 'mf.map_weight',
- 'm.left_primer', 'm.right_primer', 'm.min_primer_dist',
- 'm.max_primer_dist', 'm.priority', 'm.type', 'ms.marker_synonym_id',
- 'ms.name', 'ms.source');
+ return ('mf.marker_feature_id', 'mf.marker_id',
+ 'mf.seq_region_id', 'mf.seq_region_start', 'mf.seq_region_end',
+ 'mf.analysis_id', 'mf.map_weight',
+ 'm.left_primer', 'm.right_primer', 'm.min_primer_dist',
+ 'm.max_primer_dist', 'm.priority', 'm.type', 'ms.marker_synonym_id',
+ 'ms.name', 'ms.source');
}
sub _tables {
my $self = shift;
return (['marker_feature', 'mf'], #primary table
- ['marker', 'm'],
- ['marker_synonym', 'ms']);
+ ['marker', 'm'],
+ ['marker_synonym', 'ms']);
}
sub _left_join {
my $self = shift;
- return ('marker_synonym',
- 'ON m.display_marker_synonym_id = ms.marker_synonym_id');
+ return ( [ 'marker_synonym',
+ 'm.display_marker_synonym_id = ms.marker_synonym_id' ] );
}
-
+
sub _default_where_clause {
my $self = shift;
@@ -170,18 +151,17 @@ sub _default_where_clause {
}
sub _objs_from_sth {
- my $self = shift;
- my $sth = shift;
+ my ($self, $sth, $mapper, $dest_slice) = @_;
my ($marker_feature_id, $marker_id,
- $contig_id, $contig_start, $contig_end,
+ $seq_region_id, $seq_region_start, $seq_region_end,
$analysis_id, $map_weight,
- $left_primer, $right_primer, $min_primer_dist, $max_primer_dist,
+ $left_primer, $right_primer, $min_primer_dist, $max_primer_dist,
$priority, $type, $ms_id, $ms_name, $ms_source);
#warning: ordering depends on _columns function implementation
- $sth->bind_columns(\$marker_feature_id, \$marker_id,
- \$contig_id, \$contig_start, \$contig_end,
+ $sth->bind_columns(\$marker_feature_id, \$marker_id,
+ \$seq_region_id, \$seq_region_start, \$seq_region_end,
\$analysis_id, \$map_weight,
\$left_primer, \$right_primer, \$min_primer_dist, \$max_primer_dist,
\$priority, \$type, \$ms_id, \$ms_name, \$ms_source);
@@ -189,47 +169,130 @@ sub _objs_from_sth {
my @out = ();
my %marker_cache;
- my %contig_cache;
+ my %slice_hash;
+ my %sr_name_hash;
+ my %sr_cs_hash;
my %analysis_cache;
my $marker_adp = $self->db->get_MarkerAdaptor;
- while($sth->fetch) {
+ my $sa = $self->db->get_SliceAdaptor;
+ my $analysis_adp = $self->db->get_AnalysisAdaptor;
+
+ my $asm_cs;
+ my $cmp_cs;
+ my $asm_cs_vers;
+ my $asm_cs_name;
+ my $cmp_cs_vers;
+ my $cmp_cs_name;
+ if($mapper) {
+ $asm_cs = $mapper->assembled_CoordSystem();
+ $cmp_cs = $mapper->component_CoordSystem();
+ $asm_cs_name = $asm_cs->name();
+ $asm_cs_vers = $asm_cs->version();
+ $cmp_cs_name = $cmp_cs->name();
+ $cmp_cs_vers = $cmp_cs->version();
+ }
+ my $dest_slice_start;
+ my $dest_slice_end;
+ my $dest_slice_strand;
+ my $dest_slice_length;
+ if($dest_slice) {
+ $dest_slice_start = $dest_slice->start();
+ $dest_slice_end = $dest_slice->end();
+ $dest_slice_strand = $dest_slice->strand();
+ $dest_slice_length = $dest_slice->length();
+ }
+
+ FEATURE: while($sth->fetch) {
#create a new marker unless this one has been seen already
my $marker;
- unless($marker = $marker_cache{$marker_id}) {
+ if(!($marker = $marker_cache{$marker_id})) {
#create a new marker synonym for the display synonym (if defined)
my $ms;
if($ms_id) {
- $ms = Bio::EnsEMBL::Map::MarkerSynonym->new
- ($ms_id, $ms_source, $ms_name);
+ $ms = Bio::EnsEMBL::Map::MarkerSynonym->new
+ ($ms_id, $ms_source, $ms_name);
}
#create a new marker
$marker = Bio::EnsEMBL::Map::Marker->new
- ($marker_id, $marker_adp,
- $left_primer, $right_primer, $min_primer_dist, $max_primer_dist,
- $priority, $type, $ms);
+ ($marker_id, $marker_adp,
+ $left_primer, $right_primer, $min_primer_dist, $max_primer_dist,
+ $priority, $type, $ms);
$marker_cache{$marker_id} = $marker;
}
- #retrieve the contig from the database
- my $contig;
- unless($contig = $contig_cache{$contig_id}) {
- $contig = $self->db->get_RawContigAdaptor->fetch_by_dbID($contig_id);
- $contig_cache{$contig_id} = $contig;
+ #get the slice object
+ my $slice = $slice_hash{"ID:".$seq_region_id};
+
+ if(!$slice) {
+ $slice = $sa->fetch_by_seq_region_id($seq_region_id);
+ $slice_hash{"ID:".$seq_region_id} = $slice;
+ $sr_name_hash{$seq_region_id} = $slice->seq_region_name();
+ $sr_cs_hash{$seq_region_id} = $slice->coord_system();
}
#retrieve analysis
my $analysis;
unless($analysis = $analysis_cache{$analysis_id}) {
- $analysis = $self->db->get_AnalysisAdaptor->fetch_by_dbID($analysis_id);
+ $analysis = $analysis_adp->fetch_by_dbID($analysis_id);
$analysis_cache{$analysis_id} = $analysis;
}
+ #
+ # remap the feature coordinates to another coord system
+ # if a mapper was provided
+ #
+ if($mapper) {
+ my $sr_name = $sr_name_hash{$seq_region_id};
+ my $sr_cs = $sr_cs_hash{$seq_region_id};
+
+ ($sr_name,$seq_region_start,$seq_region_end) =
+ $mapper->fastmap($sr_name, $seq_region_start, $seq_region_end,
+ 0, $sr_cs);
+
+ #skip features that map to gaps or coord system boundaries
+ next FEATURE if(!defined($sr_name));
+
+ #get a slice in the coord system we just mapped to
+ if($asm_cs == $sr_cs || ($cmp_cs != $sr_cs && $asm_cs->equals($sr_cs))) {
+ $slice = $slice_hash{"NAME:$sr_name:$cmp_cs_name:$cmp_cs_vers"} ||=
+ $sa->fetch_by_region($cmp_cs_name, $sr_name,undef, undef, undef,
+ $cmp_cs_vers);
+ } else {
+ $slice = $slice_hash{"NAME:$sr_name:$asm_cs_name:$asm_cs_vers"} ||=
+ $sa->fetch_by_region($asm_cs_name, $sr_name, undef, undef, undef,
+ $asm_cs_vers);
+ }
+ }
+
+ #
+ # If a destination slice was provided convert the coords
+ # If the dest_slice starts at 1 and is foward strand, nothing needs doing
+ #
+ if($dest_slice) {
+ if($dest_slice_start != 1 || $dest_slice_strand != 1) {
+ if($dest_slice_strand == 1) {
+ $seq_region_start = $seq_region_start - $dest_slice_start + 1;
+ $seq_region_end = $seq_region_end - $dest_slice_start + 1;
+ } else {
+ my $tmp_seq_region_start = $seq_region_start;
+ $seq_region_start = $dest_slice_end - $seq_region_end + 1;
+ $seq_region_end = $dest_slice_end - $tmp_seq_region_start + 1;
+ }
+
+ #throw away features off the end of the requested slice
+ if($seq_region_end < 1 || $seq_region_start > $dest_slice_length) {
+ next FEATURE;
+ }
+ }
+ $slice = $dest_slice;
+ }
+
#now create a new marker_feature using the marker
push @out, Bio::EnsEMBL::Map::MarkerFeature->new
- ($marker_feature_id, $self,
- $contig_start, $contig_end, $contig,
+ ($marker_feature_id, $self,
+ $seq_region_start, $seq_region_end, $slice,
$analysis, $marker_id, $map_weight, $marker);
}
@@ -256,74 +319,61 @@ sub _objs_from_sth {
sub store {
my ($self, @mfs) = @_;
+ my $analysis_adaptor = $self->db->get_AnalysisAdaptor();
+
foreach my $mf (@mfs) {
#
# Sanity checking!
#
- unless($mf && ref $mf && $mf->isa('Bio::EnsEMBL::Map::MarkerFeature')) {
+ if(!ref($mf) || !$mf->isa('Bio::EnsEMBL::Map::MarkerFeature')) {
$self->throw('Incorrect argument [$mf] to store. Expected ' .
- 'Bio::EnsEMBL::Map::MarkerFeature');
- }
-
- #don't store this feature if it has already been stored
- next if($mf->adaptor && ($mf->adaptor == $self));
-
- my $marker = $mf->marker;
-
- unless($marker && ref $marker &&
- $marker->isa('Bio::EnsEMBL::Map::Marker')) {
- $self->throw('Cannot store MarkerFeature without an associated Marker');
+ 'Bio::EnsEMBL::Map::MarkerFeature');
}
- my $marker_id = $marker->dbID;
- unless($marker_id) {
- $self->throw('Associated marker must have dbID to store MarkerFeature');
+ #don't store this feature if it has already been stored
+ if($mf->is_stored($self->db())) {
+ warning('MarkerFeature ['.$mf->dbID.'] is already stored in this DB.');
}
+ my $marker = $mf->marker;
- my $analysis = $mf->analysis;
-
- unless($analysis && ref $analysis &&
- $analysis->isa('Bio::EnsEMBL::Analysis')) {
- $self->throw('Cannot store MarkerFeature without associated Analysis');
+ if(!$marker || !ref($marker) ||
+ !$marker->isa('Bio::EnsEMBL::Map::Marker')) {
+ throw('Cannot store MarkerFeature without an associated Marker');
}
- my $analysis_id = $analysis->dbID;
-
- unless($analysis_id) {
- $self->throw('Associated analysis must have dbID to store MarkerFeature');
+ my $marker_id = $marker->dbID;
+ if(!$marker_id) {
+ throw('Associated marker must have dbID to store MarkerFeature');
}
- my $contig = $mf->contig;
+ my $analysis = $mf->analysis;
- unless($contig && ref $contig && $contig->isa('Bio::EnsEMBL::RawContig')) {
- $self->throw('Cannot store MarkerFeature that is not in contig coords');
+ #store the analysis if it has not been stored yet
+ if(!$analysis->is_stored($self->db())) {
+ $analysis_adaptor->store($mf->analysis());
}
-
- my $contig_id = $contig->dbID;
- unless($contig_id) {
- $self->throw('Attached contig must have dbID to store MarkerFeature');
- }
+ my $analysis_id = $analysis->dbID;
- #
- # Everything looks ok so store
- #
+ my $original = $mf;
+ my $seq_region_id;
+ ($mf, $seq_region_id) = $self->_pre_store($mf);
- my $sth =
+ my $sth =
$self->prepare("INSERT INTO marker_feature (marker_id,
- contig_id, contig_start, contig_end,
+ seq_region_id, seq_region_start, seq_region_end,
analysis_id, map_weight)
- VALUES (?, ?, ?, ?, ?, ?)");
- $sth->execute($marker_id,
- $contig_id, $mf->start, $mf->end,
- $analysis_id, 0);
+ VALUES (?, ?, ?, ?, ?, ?)");
+ $sth->execute($marker_id,
+ $seq_region_id, $mf->start, $mf->end,
+ $analysis_id, 0);
my $dbID = $sth->{'mysql_insertid'};
-
- $mf->dbID($dbID);
- $mf->adaptor($self);
+
+ $original->dbID($dbID);
+ $original->adaptor($self);
}
}
diff --git a/modules/Bio/EnsEMBL/Map/DBSQL/QtlFeatureAdaptor.pm b/modules/Bio/EnsEMBL/Map/DBSQL/QtlFeatureAdaptor.pm
index c7e304db61..5a7ae5e285 100644
--- a/modules/Bio/EnsEMBL/Map/DBSQL/QtlFeatureAdaptor.pm
+++ b/modules/Bio/EnsEMBL/Map/DBSQL/QtlFeatureAdaptor.pm
@@ -53,9 +53,9 @@ use vars qw(@ISA);
sub fetch_all_by_Qtl {
my $self = shift;
my $qtl = shift;
-
+
my $constraint = 'q.qtl_id = ' . $qtl->dbID;
-
+
return $self->generic_fetch($constraint, @_);
}
@@ -65,68 +65,71 @@ sub fetch_all_by_Qtl {
sub _columns {
my $self = shift;
- return ( 'c.name', 'qf.start', 'qf.end', 'q.qtl_id', 'qf.analysis_id',
- 'qs.source_database', 'qs.source_primary_id',
- 'q.trait', 'q.lod_score', 'q.flank_marker_id_1',
- 'q.flank_marker_id_2', 'q.peak_marker_id' );
+ return ( 'qf.seq_region_id', 'qf.seq_region_start', 'qf.seq_region_end',
+ 'q.qtl_id',
+ 'qf.analysis_id',
+ 'qs.source_database', 'qs.source_primary_id',
+ 'q.trait', 'q.lod_score', 'q.flank_marker_id_1',
+ 'q.flank_marker_id_2', 'q.peak_marker_id' );
}
sub _tables {
my $self = shift;
return (['qtl_feature', 'qf'], #primary table
- ['qtl', 'q'],
- ['chromosome' ,'c' ],
+ ['qtl', 'q'],
['qtl_synonym', 'qs']);
}
sub _left_join {
- return ('qtl_synonym', 'ON q.qtl_id = qs.qtl_id');
+ return ( [ 'qtl_synonym', 'q.qtl_id = qs.qtl_id' ] );
}
-
+
sub _default_where_clause {
my $self = shift;
- return ('qf.qtl_id = q.qtl_id AND qf.chromosome_id = c.chromosome_id');
+ return ('qf.qtl_id = q.qtl_id');
}
sub _objs_from_sth {
my $self = shift;
my $sth = shift;
- my $mapper = shift;
- my $slice = shift; # optional Slice
-
-
- my ( $chromosome_name, $chr_start, $chr_end, $qtl_id, $analysis_id,
- $source_database,
+ my ( $seq_region_id, $seq_region_start, $seq_region_end, $qtl_id,
+ $analysis_id, $source_database,
$source_primary_id, $trait, $lod_score, $flank_marker_id_1,
$flank_marker_id_2, $peak_marker_id );
#warning: ordering depends on _columns function implementation
- $sth->bind_columns( \$chromosome_name, \$chr_start, \$chr_end, \$qtl_id,
- \$analysis_id,
- \$source_database, \$source_primary_id, \$trait,
- \$lod_score, \$flank_marker_id_1,
- \$flank_marker_id_2, \$peak_marker_id );
+ $sth->bind_columns( \$seq_region_id, \$seq_region_start, \$seq_region_end,
+ \$qtl_id, \$analysis_id,
+ \$source_database, \$source_primary_id, \$trait,
+ \$lod_score, \$flank_marker_id_1,
+ \$flank_marker_id_2, \$peak_marker_id );
my @out = ();
my %already_seen;
+
+ my $mad = $self->db()->get_MarkerAdaptor();
+ my $aad = $self->db()->get_AnalysisAdaptor();
+ my $sad = $self->db()->get_SliceAdaptor();
+
while( $sth->fetch()) {
- my $mad = $self->db()->get_MarkerAdaptor();
+ my $flank_marker_1 = $flank_marker_id_1 ?
+ $mad->fetch_by_dbID( $flank_marker_id_1 ) :
+ undef;
+ my $flank_marker_2 = $flank_marker_id_2 ?
+ $mad->fetch_by_dbID( $flank_marker_id_2 ) :
+ undef;
+ my $peak_marker = $peak_marker_id ?
+ $mad->fetch_by_dbID( $peak_marker_id ) :
+ undef;
- my $flank_marker_1 = $flank_marker_id_1 ? $mad->fetch_by_dbID( $flank_marker_id_1 ) : undef;
- my $flank_marker_2 = $flank_marker_id_2 ? $mad->fetch_by_dbID( $flank_marker_id_2 ) : undef;
- my $peak_marker = $peak_marker_id ? $mad->fetch_by_dbID( $peak_marker_id ) : undef;
-
- my $analysis = $self->db()->get_AnalysisAdaptor()->fetch_by_dbID( $analysis_id );
+ my $analysis = $aad->fetch_by_dbID( $analysis_id );
- if( ! $slice ) {
- $slice = $self->db->get_SliceAdaptor()->
- fetch_by_chr_name( $chromosome_name );
- }
+ my $slice = $sad->fetch_by_seq_region_id($seq_region_id);
#rows with the same qtl contain additional synonyms of the qtl
if(my $qtl = $already_seen{$qtl_id}) {
@@ -147,95 +150,19 @@ sub _objs_from_sth {
);
$already_seen{$qtl_id} = $qtl;
-
+
#now create a new marker_feature using the marker
- if( $slice->strand == 1 ) {
- push @out, Bio::EnsEMBL::Map::QtlFeature->new
- (
- $self,
- $slice,
- $chr_start - $slice->chr_start() + 1,
- $chr_end - $slice->chr_start() + 1,
- $qtl,
- $analysis
- );
- } else {
- push @out, Bio::EnsEMBL::Map::QtlFeature->new
- (
- $self,
- $slice,
- $slice->chr_end() - $chr_end + 1,
- $slice->chr_end() - $chr_start + 1,
- $qtl,
- $analysis
- );
- }
+ push @out, Bio::EnsEMBL::Map::QtlFeature->new
+ ($self,
+ $slice,
+ $seq_region_start,
+ $seq_region_end,
+ $qtl,
+ $analysis);
}
return \@out;
}
-=head2 fetch_all_by_Slice_constraint
-
- Arg [1] : Bio::EnsEMBL::Slice $slice
- the slice from which to obtain features
- Arg [2] : (optional) string $constraint
- An SQL query constraint (i.e. part of the WHERE clause)
- Arg [3] : (optional) string $logic_name
- the logic name of the type of features to obtain
- Example : $fs = $a->fetch_all_by_Slice_constraint($slc, 'perc_ident > 5');
- Description: Returns a listref of features created from the database which
- are on the Slice defined by $slice and fulfill the SQL
- constraint defined by $constraint. If logic name is defined,
- only features with an analysis of type $logic_name will be
- returned.
- Returntype : listref of Bio::EnsEMBL::SeqFeatures in Slice coordinates
- Exceptions : thrown if $slice is not defined
- Caller : Bio::EnsEMBL::Slice
-
-=cut
-
-sub fetch_all_by_Slice_constraint {
- my($self, $slice, $constraint, $logic_name) = @_;
-
- unless(defined $slice && ref $slice && $slice->isa("Bio::EnsEMBL::Slice")) {
- $self->throw("Slice arg must be a Bio::EnsEMBL::Slice not a [$slice]\n");
- }
-
- $logic_name = '' unless $logic_name;
- $constraint = '' unless $constraint;
-
- #check the cache and return if we have already done this query
- my $key = join($slice->name, $constraint, $logic_name);
- return $self->{'_slice_feature_cache'}{$key}
- if $self->{'_slice_feature_cache'}{$key};
-
- my $slice_start = $slice->chr_start();
- my $slice_end = $slice->chr_end();
-
- #get the synonym of the primary_table
- my @tabs = $self->_tables;
- my $syn = $tabs[0]->[1];
-
- my $chromosome_id = $slice->get_Chromosome->dbID();
-
- $constraint .= ' AND ' if($constraint);
-
- $constraint .= " ${syn}.chromosome_id = $chromosome_id " .
- "AND ${syn}.end >= $slice_start AND ${syn}.start <= $slice_end";
-
- #for speed the remapping to slice may be done at the time of object creation
- my $features =
- $self->generic_fetch($constraint, $logic_name, undef, $slice);
-
- if(@$features && (!$features->[0]->can('contig') ||
- $features->[0]->contig == $slice)) {
- #features have been converted to slice coords already, cache and return
- return $self->{'_slice_feature_cache'}{$key} = $features;
- }
-}
-
-
-
1;
diff --git a/modules/Bio/EnsEMBL/Map/MapLocation.pm b/modules/Bio/EnsEMBL/Map/MapLocation.pm
index e6f2795751..af8d38844c 100644
--- a/modules/Bio/EnsEMBL/Map/MapLocation.pm
+++ b/modules/Bio/EnsEMBL/Map/MapLocation.pm
@@ -27,6 +27,7 @@ use strict;
use vars qw(@ISA);
use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
@ISA = qw(Bio::EnsEMBL::Root);
@@ -35,14 +36,14 @@ use Bio::EnsEMBL::Root;
Arg [1] : (optional) string $name
Arg [2] : (optional) string $map_name
- Arg [3] : (optional) Bio::EnsEMBL::Chromosome $chromosome
+ Arg [3] : (optional) string $chromosome_name
Arg [4] : (optional) string $position
Arg [5] : (optional) float $lod_score
Example : $map_location = Bio::EnsEMBL::Map::MapLocation('DS1234',
- 'genethon',
- $chr,
- '12.39',
- 50.12);
+ 'genethon',
+ 'X',
+ '12.39',
+ 50.12);
Description: Creates a new MapLocation
Returntype : Bio::EnsEMBL::Map::MapLocation
Exceptions : none
@@ -51,15 +52,15 @@ use Bio::EnsEMBL::Root;
=cut
sub new {
- my ($caller, $name, $map_name, $chromosome, $position, $lod_score) = @_;
+ my ($caller, $name, $map_name, $chromosome_name, $position, $lod_score) = @_;
my $class = ref($caller) || $caller;
- return bless( {'map_name' => $map_name,
- 'name' => $name,
- 'chromosome' => $chromosome,
- 'position' => $position,
- 'lod_score' => $lod_score}, $class );
+ return bless( {'map_name' => $map_name,
+ 'name' => $name,
+ 'chromosome_name' => $chromosome_name,
+ 'position' => $position,
+ 'lod_score' => $lod_score}, $class );
}
@@ -68,20 +69,16 @@ sub new {
Arg [1] : string $map_name
Example : $map_name = $map_location->map_name;
- Description: Retrieves the map_name
- Returntype :
- Exceptions :
- Caller :
+ Description: Getter/Setter for the map name
+ Returntype : string
+ Exceptions : none
+ Caller : general
=cut
sub map_name {
my $self = shift;
-
- if(@_) {
- $self->{'map_name'} = shift;
- }
-
+ $self->{'map_name'} = shift if(@_);
return $self->{'map_name'};
}
@@ -93,7 +90,7 @@ sub map_name {
Example : $name = $map_location->name;
Description: A name associated with the marker at this position. For
example if this is a genethon map location the name will be
- the synonym of source genethon.
+ the synonym of source genethon.
Returntype : string
Exceptions : none
Caller : general
@@ -102,39 +99,27 @@ sub map_name {
sub name {
my $self = shift;
-
- if(@_) {
- $self->{'name'} = shift;
- }
-
- return $self->{'name'};
+ $self->{'name'} = shift if(@_);
+ return $self->{'name'};
}
-=head2 chromosome
+=head2 chromosome_name
- Arg [1] : (optional) Bio::EnsEMBL::Chromosome
- Example : $chr = $map_location->chromosome;
- Description: Getter/Setter for the chromosome of this map location
- Returntype : Bio::EnsEMBL::Chromosome
- Exceptions : thrown if an invalid arg is passed
+ Arg [1] : (optional) string $chromosome_name
+ Example : $chr_name = $map_location->chromosome_name;
+ $map_location->chromosome_name('X');
+ Description: The name of the chromosome associated with this map location
+ Returntype : string
+ Exceptions : none
Caller : general
=cut
-sub chromosome {
+sub chromosome_name{
my $self = shift;
-
- if(@_) {
- my $chr = shift;
- if($chr && !(ref($chr) && $chr->isa('Bio::EnsEMBL::Chromosome'))) {
- $self->throw("arg must be a Bio::EnsEMBL::Chromosome not [$chr]");
- }
-
- $self->{'chromosome'} = $chr;
- }
-
- return $self->{'chromosome'};
+ $self->{'chromosome_name'} = shift if(@_);
+ return $self->{'chromosome_name'};
}
@@ -152,11 +137,7 @@ sub chromosome {
sub position {
my $self = shift;
-
- if(@_) {
- $self->{'position'} = shift;
- }
-
+ $self->{'position'} = shift if(@_);
return $self->{'position'};
}
@@ -175,13 +156,37 @@ sub position {
sub lod_score {
my $self = shift;
+ $self->{'lod_score'} = shift if(@_);
+ return $self->{'lod_score'};
+}
+
+
+
+=head2 chromosome
+
+ Description: DEPRECATED use chromosome_name() instead
+
+=cut
+
+sub chromosome {
+ my $self = shift;
+ deprecate('use chromosome_name instead');
if(@_) {
- $self->{'lod_score'} = shift;
+ my $chr = shift;
+ if(ref($chr)) {
+ $self->chromosome_name($chr->seq_region_name());
+ } else {
+ $self->chromosome_name($chr);
+ }
}
- return $self->{'lod_score'};
+ #this object has no way to talk to db and thus no way to
+ #get a chromosome object
+ return $self->chromosome_name();
}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/Map/Marker.pm b/modules/Bio/EnsEMBL/Map/Marker.pm
index e52192e786..4749b6414c 100644
--- a/modules/Bio/EnsEMBL/Map/Marker.pm
+++ b/modules/Bio/EnsEMBL/Map/Marker.pm
@@ -27,9 +27,10 @@ package Bio::EnsEMBL::Map::Marker;
use strict;
use vars qw(@ISA);
-use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Storable;
+use Bio::EnsEMBL::Utils::Exception qw(throw);
-@ISA = qw(Bio::EnsEMBL::Root);
+@ISA = qw(Bio::EnsEMBL::Storable);
@@ -63,16 +64,16 @@ sub new {
my $class = ref($caller) || $caller;
- my $self = bless( {'dbID' => $dbID,
- 'adaptor' => $adaptor,
- 'left_primer' => $left_primer,
- 'right_primer' => $right_primer,
- 'min_primer_dist' => $min_primer_dist,
- 'max_primer_dist' => $max_primer_dist,
- 'priority' => $priority,
- 'type' => $type,
- 'display_marker_synonym' => $display_synonym
- }, $class);
+ my $self = bless( {'dbID' => $dbID,
+ 'adaptor' => $adaptor,
+ 'left_primer' => $left_primer,
+ 'right_primer' => $right_primer,
+ 'min_primer_dist' => $min_primer_dist,
+ 'max_primer_dist' => $max_primer_dist,
+ 'priority' => $priority,
+ 'type' => $type,
+ 'display_marker_synonym' => $display_synonym
+ }, $class);
#only load the marker synononyms if they were supplied, otherwise they
# will be lazy-loaded
@@ -91,52 +92,6 @@ sub new {
return $self;
}
-=head2 dbID
-
- Arg [1] : (optional) $dbID
- Example : $dbID = $marker->dbID;
- Description: Getter/Setter for the internal identifier of this marker
- Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub dbID {
- my $self = shift;
-
- if(@_) {
- $self->{'dbID'} = shift;
- }
-
- return $self->{'dbID'};
-}
-
-
-
-=head2 adaptor
-
- Arg [1] : (optional) Bio::EnsEMBL::Map::DBSQL::MarkerAdaptor $adaptor
- Example : $adaptor = $marker->adaptor;
- Description: Getter/Setter for the adaptor that performs database interaction
- for this Marker.
- Returntype : Bio::EnsEMBL::Map::DBSQL::MarkerAdaptor
- Exceptions : none
- Caller : general
-
-=cut
-
-sub adaptor {
- my $self = shift;
-
- if(@_) {
- $self->{'adaptor'} = shift;
- }
-
- return $self->{'adaptor'};
-}
-
-
=head2 left_primer
@@ -372,7 +327,7 @@ sub display_MarkerSynonym {
if(@_) {
my $ms = shift;
if($ms && !(ref $ms && $ms->isa('Bio::EnsEMBL::Map::MarkerSynonym'))) {
- $self->throw("ms arg must be Bio::EnsEMBL::Map::MarkerSynonym not [$ms]");
+ throw("ms arg must be Bio::EnsEMBL::Map::MarkerSynonym not [$ms]");
}
$self->{'display_marker_synonym'} = $ms;
}
@@ -459,7 +414,7 @@ sub get_MapLocation {
}
unless($map_name) {
- $self->throw('map_name argument is required, or use get_all_MapLocations');
+ throw('map_name argument is required, or use get_all_MapLocations');
}
return $self->{'map_locations'}->{$map_name};
@@ -485,12 +440,12 @@ sub add_MapLocations {
foreach my $ml (@mlocs) {
unless($ml && ref $ml && $ml->isa('Bio::EnsEMBL::Map::MapLocation')) {
- $self->throw("args must be Bio::EnsEMBL::Map::MapLocations not [$ml]");
+ throw("args must be Bio::EnsEMBL::Map::MapLocations not [$ml]");
}
my $mname = $ml->map_name;
unless($mname) {
- $self->throw("map location arg [$ml] does not define a map name");
+ throw("map location arg [$ml] does not define a map name");
}
$self->{'map_locations'}->{$mname} = $ml;
@@ -517,7 +472,7 @@ sub flush_MapLocations{
delete $self->{'map_locations'};
}
-
+
1;
diff --git a/modules/Bio/EnsEMBL/Map/MarkerFeature.pm b/modules/Bio/EnsEMBL/Map/MarkerFeature.pm
index f706c5e1e8..7a3577ab57 100644
--- a/modules/Bio/EnsEMBL/Map/MarkerFeature.pm
+++ b/modules/Bio/EnsEMBL/Map/MarkerFeature.pm
@@ -27,9 +27,9 @@ package Bio::EnsEMBL::Map::MarkerFeature;
use strict;
use vars qw(@ISA);
-use Bio::EnsEMBL::SeqFeature;
+use Bio::EnsEMBL::Feature;
-@ISA = qw(Bio::EnsEMBL::SeqFeature);
+@ISA = qw(Bio::EnsEMBL::Feature);
@@ -37,9 +37,9 @@ use Bio::EnsEMBL::SeqFeature;
Arg [1] : (optional) int $dbID
Arg [2] : (optional) Bio::EnsEMBL::Adaptor $adaptor
- Arg [3] : (optional) int $start
+ Arg [3] : (optional) int $start
Arg [4] : (optional) int $end
- Arg [5] : (optional) Bio::EnsEMBL ::RawContig or ::Slice $contig
+ Arg [5] : (optional) Bio::EnsEMBL::Slice $slice
Arg [6] : (optional) Bio::EnsEMBL::Analysis
Arg [7] : (optional) int $marker_id
Arg [8] : (optional) int $map_weight
@@ -55,70 +55,22 @@ use Bio::EnsEMBL::SeqFeature;
=cut
sub new {
- my ($caller, $dbID, $adaptor, $start, $end, $contig, $analysis,
+ my ($caller, $dbID, $adaptor, $start, $end, $slice, $analysis,
$marker_id, $map_weight, $marker) = @_;
-
+
my $class = ref($caller) || $caller;
return bless( {
'dbID' => $dbID,
'adaptor' => $adaptor,
- '_gsf_start' => $start,
- '_gsf_end' => $end,
- '_gsf_strand' => 0,
- '_gsf_seq' => $contig,
- '_analysis' => $analysis,
+ 'start' => $start,
+ 'end' => $end,
+ 'strand' => 0,
+ 'slice' => $slice,
+ 'analysis' => $analysis,
'marker_id' => $marker_id,
'marker' => $marker,
- 'map_weight' => $map_weight }, $class);
-}
-
-
-
-
-=head2 dbID
-
- Arg [1] : (optional) int $dbID
- Example : $dbID = $marker_feature->dbID;
- Description: Getter/Setter for the dbID of this MarkerFeature
- Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub dbID {
- my $self = shift;
-
- if(@_) {
- $self->{'dbID'} = shift;
- }
-
- return $self->{'dbID'};
-}
-
-
-
-=head2 adaptor
-
- Arg [1] : (optional) Bio::EnsEMBL::Map::DBSQL::MarkerFeatureAdaptor $adp
- Example : $adaptor = $marker_feature->adaptor;
- Description: Getter/Setter for the adaptor which provides database
- database interaction for this MarkerFeature
- Returntype : Bio::EnsEMBL::Map::DBSQL::MarkerFeatureAdaptor
- Exceptions : none
- Caller : general
-
-=cut
-
-sub adaptor {
- my $self = shift;
-
- if(@_) {
- $self->{'adaptor'} = shift;
- }
-
- return $self->{'adaptor'};
+ 'map_weight' => $map_weight }, $class);
}
@@ -162,7 +114,7 @@ sub _marker_id {
sub marker {
my $self = shift;
-
+
if(@_) {
$self->{'marker'} = shift;
} elsif(!$self->{'marker'} && $self->{'adaptor'} && $self->{'marker_id'}) {
@@ -170,7 +122,7 @@ sub marker {
my $ma = $self->{'adaptor'}->db->get_MarkerAdaptor;
$self->{'marker'} = $ma->fetch_by_dbID($self->{'marker_id'});
}
-
+
return $self->{'marker'};
}
@@ -200,4 +152,30 @@ sub map_weight {
return $self->{'map_weight'};
}
+
+
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $mf->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For marker features this is the
+ name of the display synonym or '' if it is not defined.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
+
+=cut
+
+sub display_id {
+ my $self = shift;
+ my $marker = $self->{'marker'};
+
+ return '' if(!$marker);
+ my $ms = $marker->display_MarkerSynonym();
+ return '' if(!$ms);
+ return $ms->name() || '';
+}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/Map/Qtl.pm b/modules/Bio/EnsEMBL/Map/Qtl.pm
index da678726a3..9f1f492d81 100644
--- a/modules/Bio/EnsEMBL/Map/Qtl.pm
+++ b/modules/Bio/EnsEMBL/Map/Qtl.pm
@@ -36,9 +36,10 @@ package Bio::EnsEMBL::Map::Qtl;
use strict;
use vars qw(@ISA);
-use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Storable;
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate);
-@ISA = qw(Bio::EnsEMBL::Root);
+@ISA = qw(Bio::EnsEMBL::Storable);
@@ -62,71 +63,26 @@ use Bio::EnsEMBL::Root;
=cut
sub new {
- my ( $class, $dbID, $adaptor, $flank_marker_1, $peak_marker,
+ my ( $class, $dbID, $adaptor, $flank_marker_1, $peak_marker,
$flank_marker_2, $trait, $lod_score,
$synonyms ) = @_;
$class = ref( $class ) ||$class;
my $self = bless( {
- 'dbID' => $dbID,
- 'adaptor' => $adaptor,
+ 'dbID' => $dbID,
+ 'adaptor' => $adaptor,
'flank_marker_1' => $flank_marker_1,
'flank_marker_2' => $flank_marker_2,
- 'peak_marker' => $peak_marker,
- 'trait' => $trait,
- 'lod_score' => $lod_score,
- 'synonyms' => $synonyms
+ 'peak_marker' => $peak_marker,
+ 'trait' => $trait,
+ 'lod_score' => $lod_score,
+ 'synonyms' => $synonyms
}, $class );
- return $self;
-}
-
-
-
-=head2 dbID
-
- Arg [1] : int $dbID
- Example : none
- Description: get/set/clear attribute dbID
- Returntype : int
- Exceptions : none
- Caller : DBSQL::QtlAdaptor
-
-=cut
-
-sub dbID {
- my $self = shift;
-
- if(@_) {
- $self->{'dbID'} = shift;
- }
-
- return $self->{'dbID'};
-}
-
-
-=head2 adaptor
- Arg [1] : Bio::EnsEMBL::Map::DBSQL::QtlAdaptor $adaptor
- Example : none
- Description: Getter/Setter attribute adaptor
- Returntype : Bio::EnsEMBL::Map::DBSQL::QtlAdaptor
- Exceptions : none
- Caller : DBSQL::QtlAdaptor
-
-=cut
-
-sub adaptor {
- my $self = shift;
-
- if(@_) {
- $self->{'adaptor'} = shift;
- }
-
- return $self->{'adaptor'};
+ return $self;
}
-
=head2 add_synonym
Arg [1] : string $source
@@ -145,9 +101,9 @@ sub add_synonym {
my $self = shift;
my $source = shift;
my $identifier = shift;
-
+
unless($source && $identifier) {
- $self->throw('Source and identifier arguments are required');
+ throw('Source and identifier arguments are required');
}
$self->{'synonyms'}->{$source} = $identifier;
@@ -157,7 +113,7 @@ sub add_synonym {
=head2 get_synonyms
Arg [1] : none
- Example :
+ Example :
foreach my $source ($keys %{$qtl->get_synonyms}) {
print $source . ':'. $qtl->get_synonyms->{$source};
}
@@ -338,14 +294,11 @@ This method is deprecated. Use get_synonyms or add_synonym instead.
sub source_database {
my $self = shift;
- my ($f, $p, $l) = caller;
-
- $self->warn("$f:$l: call to deprecated method Qtl::source_database.".
- " Use Qtl::get_synonyms or Qtl::add_synonym instead");
+ deprecate('Use get_synonyms or add_synonym instead');
my $syns = $self->get_synonyms;
my ($source) = keys %$syns;
-
+
return $source || '';
}
@@ -359,13 +312,11 @@ This method is deprecated. Use get_synonyms or add_synonym instead.
sub source_primary_id {
my $self = shift;
- my ($f, $p, $l) = caller;
- $self->warn("$f:$l: call to deprecated method Qtl::source_database.".
- " Use Qtl::get_synonyms or Qtl::add_synonym instead");
+ deprecate('Use get_synonyms or add_synonym instead');
my $syns = $self->get_synonyms;
my ($source) = keys %$syns;
-
+
if($source) {
return $syns->{$source};
}
diff --git a/modules/Bio/EnsEMBL/Map/QtlFeature.pm b/modules/Bio/EnsEMBL/Map/QtlFeature.pm
index e5f04b7b8d..11ccedcfed 100644
--- a/modules/Bio/EnsEMBL/Map/QtlFeature.pm
+++ b/modules/Bio/EnsEMBL/Map/QtlFeature.pm
@@ -28,8 +28,6 @@ Represents a QtlFeature in the EnsEMBL database. QtlFeatures are generally very
long and its not clear wether a representation in Contig coordinates
actually makes sense. In the database they will have chromosomal coordinates.
-
-
=cut
package Bio::EnsEMBL::Map::QtlFeature;
@@ -37,11 +35,9 @@ package Bio::EnsEMBL::Map::QtlFeature;
use strict;
use vars qw(@ISA);
-use Bio::EnsEMBL::Root;
-use Bio::EnsEMBL::SeqFeature;
-
-@ISA = qw(Bio::EnsEMBL::SeqFeature);
+use Bio::EnsEMBL::Feature;
+@ISA = qw(Bio::EnsEMBL::Feature);
=head2 new
@@ -56,42 +52,20 @@ use Bio::EnsEMBL::SeqFeature;
=cut
sub new {
- my ( $class, $adaptor, $contig, $start, $end, $qtl, $analysis ) = @_;
+ my ( $class, $adaptor, $slice, $start, $end, $qtl, $analysis ) = @_;
$class = ref( $class ) ||$class;
my $self = bless( {
- 'adaptor' => $adaptor,
- '_gsf_seq' => $contig,
- '_gsf_start' => $start,
- '_gsf_end' => $end,
- 'qtl' => $qtl,
- 'analysis' => $analysis
+ 'adaptor' => $adaptor,
+ 'slice' => $slice,
+ 'start' => $start,
+ 'end' => $end,
+ 'qtl' => $qtl,
+ 'analysis' => $analysis,
+ 'strand' => 0
}, $class );
- $self->is_splittable( 1 );
- return $self;
-}
-
-
-
-=head2 adaptor
-
- Arg [1] : Bio::EnsEMBL::Map::DBSQL::QtlFeatureAdaptor $adaptor
- Example : none
- Description: Getter/Setter attribute adaptor
- Returntype : Bio::EnsEMBL::Map::DBSQL::QtlFeatureAdaptor
- Exceptions : none
- Caller : DBSQL::QtlFeatureAdaptor
-=cut
-
-sub adaptor {
- my $self = shift;
-
- if(@_) {
- $self->{'adaptor'} = shift;
- }
-
- return $self->{'adaptor'};
+ return $self;
}
@@ -117,33 +91,13 @@ sub qtl {
return $self->{'qtl'};
}
-=head2 analysis
-
- Arg [1] : Bio::EnsEMBL::Analysis $analysis
- Example : none
- Description: getter/setter for attribute analysis
- Returntype : Bio::EnsEMBL::Analysis
- Exceptions : none
- Caller : general
-
-=cut
-
-sub analysis {
- my $self = shift;
-
- if(@_) {
- $self->{'analysis'} = shift;
- }
-
- return $self->{'analysis'};
-}
=head2 strand
Arg [1] : none
Example : $strand = $qtl_feat->strand();
- Description: Overrides the SeqFeature strand method to always return a
+ Description: Overrides the Feature strand method to always return a
value of 0 for qtl features (they are unstranded features)
Returntype : int (always 0)
Exceptions : none
@@ -153,11 +107,34 @@ sub analysis {
sub strand {
my $self = shift;
-
return 0;
}
+
+=head2 move
+
+ Arg [1] : $start - The new end of this qtl feature
+ Arg [2] : $end - The new start of this qtl feature
+ Arg [3] : $strand - ignored always set to 0
+ Example : $qtl_feat->move(1, 10_000);
+ Description: Overrides superclass move() method to ensure strand is always 0.
+ See Bio::EnsEMBL::Feature::move
+ Returntype : none
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub move {
+ my ($self, $start, $end, $strand) = @_;
+
+ #maintain a strandedness of 0
+ return $self->SUPER::move($start,$end,0);
+}
+
+
+
1;
diff --git a/modules/Bio/EnsEMBL/Mapper.pm b/modules/Bio/EnsEMBL/Mapper.pm
index a86e6ec93f..d460f96970 100644
--- a/modules/Bio/EnsEMBL/Mapper.pm
+++ b/modules/Bio/EnsEMBL/Mapper.pm
@@ -50,18 +50,15 @@ Internal methods are usually preceded with a _
=cut
package Bio::EnsEMBL::Mapper;
-use vars qw(@ISA);
use strict;
+use integer;
-
-use Bio::EnsEMBL::Root;
use Bio::EnsEMBL::Mapper::Pair;
use Bio::EnsEMBL::Mapper::Unit;
use Bio::EnsEMBL::Mapper::Coordinate;
use Bio::EnsEMBL::Mapper::Gap;
-
-@ISA = qw(Bio::EnsEMBL::Root);
+use Bio::EnsEMBL::Utils::Exception qw(throw);
sub new {
@@ -74,7 +71,7 @@ sub new {
bless $self,$class;
if( !defined $to ) {
- $self->throw("Must supply from and to tags");
+ throw("Must supply from and to tags");
}
$self->{"_pair_$from"} = {};
@@ -83,11 +80,37 @@ sub new {
$self->to($to);
$self->from($from);
+ $self->{'pair_count'} = 0;
# set stuff in self from @args
return $self;
}
+
+=head2 flush
+
+ Args : none
+ Example : none
+ Description: removes all cached information out of this mapper
+ Returntype : none
+ Exceptions : none
+ Caller : AssemblyMapper, ChainedAssemblyMapper
+
+=cut
+
+sub flush {
+ my $self = shift;
+ my $from = $self->from();
+ my $to = $self->to();
+
+ $self->{"_pair_$from"} = {};
+ $self->{"_pair_$to"} = {};
+
+ $self->{'pair_count'} = 0;
+}
+
+
+
=head2 map_coordinates
Arg 1 int $id
@@ -116,10 +139,12 @@ sub map_coordinates{
unless(defined($id) && defined($start) && defined($end) &&
defined($strand) && defined($type) ) {
- $self->throw("Must start,end,strand,id,type as coordinates");
+ throw("Must start,end,strand,id,type as coordinates");
}
+ if( ! $self->{'_is_sorted'} ) { $self->_sort() }
+
my $hash = $self->{"_pair_$type"};
my ($from, $to);
@@ -133,11 +158,7 @@ sub map_coordinates{
}
unless(defined $hash) {
- $self->throw("Type $type is neither to or from coordinate systems");
- }
-
- if( $self->{'_is_sorted'} == 0 ) {
- $self->_sort();
+ throw("Type $type is neither to or from coordinate systems");
}
if( !defined $hash->{uc($id)} ) {
@@ -149,70 +170,90 @@ sub map_coordinates{
my $last_used_pair;
my @result;
- foreach my $pair ( @{$hash->{uc($id)}} ) {
- my $self_coord = $pair->{$from};
- my $target_coord = $pair->{$to};
-
- # if we haven't even reached the start, move on
- if( $self_coord->{'end'} < $start ) {
- next;
- }
-
- # if we have over run, break
- if( $self_coord->{'start'} > $end ) {
- last;
- }
-
- if( $start < $self_coord->{'start'} ) {
- # gap detected
- my $gap = Bio::EnsEMBL::Mapper::Gap->new($start,
- $self_coord->{'start'}-1);
-
- push(@result,$gap);
- $start = $gap->{'end'}+1;
- }
+ my ( $start_idx, $end_idx, $mid_idx, $pair, $self_coord );
+ my $lr = $hash->{uc($id)};
+
+ $start_idx = 0;
+ $end_idx = $#$lr;
+
+ # binary search the relevant pairs
+ # helps if the list is big
+ while(( $end_idx - $start_idx ) > 1 ) {
+ $mid_idx = ($start_idx+$end_idx)>>1;
+ $pair = $lr->[$mid_idx];
+ $self_coord = $pair->{$from};
+ if( $self_coord->{'end'} < $start ) {
+ $start_idx = $mid_idx;
+ } else {
+ $end_idx = $mid_idx;
+ }
+ }
- my ($target_start,$target_end,$target_ori);
+ for( my $i = $start_idx; $i<=$#$lr; $i++ ) {
+ $pair = $lr->[$i];
+ my $self_coord = $pair->{$from};
+ my $target_coord = $pair->{$to};
+
+ # if we haven't even reached the start, move on
+ if( $self_coord->{'end'} < $start ) {
+ next;
+ }
+
+ # if we have over run, break
+ if( $self_coord->{'start'} > $end ) {
+ last;
+ }
+
+ if( $start < $self_coord->{'start'} ) {
+ # gap detected
+ my $gap = Bio::EnsEMBL::Mapper::Gap->new($start,
+ $self_coord->{'start'}-1);
+
+ push(@result,$gap);
+ $start = $gap->{'end'}+1;
+ }
+
+ my ($target_start,$target_end,$target_ori);
+
+ # start is somewhere inside the region
+ if( $pair->{'ori'} == 1 ) {
+ $target_start =
+ $target_coord->{'start'} + ($start - $self_coord->{'start'});
+ } else {
+ $target_end =
+ $target_coord->{'end'} - ($start - $self_coord->{'start'});
+ }
- # start is somewhere inside the region
+ # either we are enveloping this map or not. If yes, then end
+ # point (self perspective) is determined solely by target. If not
+ # we need to adjust
+
+ if( $end > $self_coord->{'end'} ) {
+ # enveloped
if( $pair->{'ori'} == 1 ) {
- $target_start =
- $target_coord->{'start'} + ($start - $self_coord->{'start'});
+ $target_end = $target_coord->{'end'};
} else {
- $target_end =
- $target_coord->{'end'} - ($start - $self_coord->{'start'});
+ $target_start = $target_coord->{'start'};
}
-
- # either we are enveloping this map or not. If yes, then end
- # point (self perspective) is determined solely by target. If not
- # we need to adjust
-
- if( $end > $self_coord->{'end'} ) {
- # enveloped
- if( $pair->{'ori'} == 1 ) {
- $target_end = $target_coord->{'end'};
- } else {
- $target_start = $target_coord->{'start'};
- }
+ } else {
+ # need to adjust end
+ if( $pair->{'ori'} == 1 ) {
+ $target_end =
+ $target_coord->{'start'} + ($end - $self_coord->{'start'});
} else {
- # need to adjust end
- if( $pair->{'ori'} == 1 ) {
- $target_end =
- $target_coord->{'start'} + ($end - $self_coord->{'start'});
- } else {
- $target_start =
- $target_coord->{'end'} - ($end - $self_coord->{'start'});
- }
+ $target_start =
+ $target_coord->{'end'} - ($end - $self_coord->{'start'});
}
+ }
- my $res = Bio::EnsEMBL::Mapper::Coordinate->new($target_coord->{'id'},
+ my $res = Bio::EnsEMBL::Mapper::Coordinate->new($target_coord->{'id'},
$target_start,
$target_end,
$pair->{'ori'} * $strand);
- push(@result,$res);
-
- $last_used_pair = $pair;
- $start = $self_coord->{'end'}+1;
+ push(@result,$res);
+
+ $last_used_pair = $pair;
+ $start = $self_coord->{'end'}+1;
}
if( !defined $last_used_pair ) {
@@ -262,6 +303,8 @@ sub fastmap {
my ($from, $to);
+ if( ! $self->{'_is_sorted'} ) { $self->_sort() }
+
if($type eq $self->{'to'}) {
$from = 'to';
$to = 'from';
@@ -271,11 +314,8 @@ sub fastmap {
}
my $hash = $self->{"_pair_$type"} or
- $self->throw("Type $type is neither to or from coordinate systems");
+ throw("Type $type is neither to or from coordinate systems");
- if( $self->{'_is_sorted'} == 0 ) {
- $self->_sort();
- }
my $pairs = $hash->{uc($id)};
@@ -325,58 +365,60 @@ sub fastmap {
Arg 7 int $end
end coordinate of 'targe' sequence
Function stores details of mapping between two regions:
- 'source' and 'target'
- Returntype none
+ 'source' and 'target'. Returns 1 if the pair was added, 0 if it
+ was already in.
+ Returntype int 0,1
Exceptions none
Caller Bio::EnsEMBL::Mapper
=cut
sub add_map_coordinates{
- my ($self, $contig_id, $contig_start, $contig_end,
- $contig_ori, $chr_name, $chr_start, $chr_end) = @_;
-
- unless(defined($contig_id) && defined($contig_start) && defined($contig_end)
- && defined($contig_ori) && defined($chr_name) && defined($chr_start)
- && defined($chr_end)) {
- $self->throw("7 arguments expected");
- }
-
- if( ($contig_end - $contig_start) != ($chr_end - $chr_start) ) {
- $self->throw("Cannot deal with mis-lengthed mappings so far");
- }
-
- my $pair = Bio::EnsEMBL::Mapper::Pair->new();
-
- my $from = Bio::EnsEMBL::Mapper::Unit->new();
- $from->start($contig_start);
- $from->end($contig_end);
- $from->id($contig_id);
-
- my $to = Bio::EnsEMBL::Mapper::Unit->new();
- $to->start($chr_start);
- $to->end($chr_end);
- $to->id($chr_name);
-
- $pair->to($to);
- $pair->from($from);
-
- $pair->ori($contig_ori);
-
- # place into hash on both ids
- my $map_to = $self->{'to'};
- my $map_from = $self->{'from'};
-
- $self->{"_pair_$map_to"}->{uc($chr_name)} ||= [];
- push(@{$self->{"_pair_$map_to"}->{uc($chr_name)}},$pair);
+ my ($self, $contig_id, $contig_start, $contig_end,
+ $contig_ori, $chr_name, $chr_start, $chr_end) = @_;
+
+ unless(defined($contig_id) && defined($contig_start) && defined($contig_end)
+ && defined($contig_ori) && defined($chr_name) && defined($chr_start)
+ && defined($chr_end)) {
+ throw("7 arguments expected");
+ }
- $self->{"_pair_$map_from"}->{uc($contig_id)} ||= [];
- push(@{$self->{"_pair_$map_from"}->{uc($contig_id)}},$pair);
+ if( ($contig_end - $contig_start) != ($chr_end - $chr_start) ) {
+ throw("Cannot deal with mis-lengthed mappings so far");
+ }
- $self->_is_sorted(0);
+ my $pair = Bio::EnsEMBL::Mapper::Pair->new();
+
+ my $from = Bio::EnsEMBL::Mapper::Unit->new();
+ $from->start($contig_start);
+ $from->end($contig_end);
+ $from->id($contig_id);
+
+ my $to = Bio::EnsEMBL::Mapper::Unit->new();
+ $to->start($chr_start);
+ $to->end($chr_end);
+ $to->id($chr_name);
+
+ $pair->to($to);
+ $pair->from($from);
+
+ $pair->ori($contig_ori);
+
+ # place into hash on both ids
+ my $map_to = $self->{'to'};
+ my $map_from = $self->{'from'};
+
+ push( @{$self->{"_pair_$map_to"}->{uc($chr_name)}}, $pair );
+ push( @{$self->{"_pair_$map_from"}->{uc($contig_id)}}, $pair );
+
+ $self->{'pair_count'}++;
+
+ $self->{'_is_sorted'} = 0;
+ return 1;
}
+
=head2 list_pairs
Arg 1 int $id
@@ -399,20 +441,16 @@ sub list_pairs{
my ($self, $id, $start, $end, $type) = @_;
+ if( ! $self->{'_is_sorted'} ) { $self->_sort() }
+
if( !defined $type ) {
- $self->throw("Must start,end,id,type as coordinates");
+ throw("Must start,end,id,type as coordinates");
}
if( $start > $end ) {
- $self->throw("Start is greater than end for id $id, start $start, end $end\n");
+ throw("Start is greater than end for id $id, start $start, end $end\n");
}
- # perhaps a little paranoid/excessive
- if( $self->_is_sorted == 0 ) {
- $self->_sort();
- }
-
-
my $hash = $self->{"_pair_$type"};
my ($from, $to);
@@ -426,7 +464,7 @@ sub list_pairs{
}
unless(defined $hash) {
- $self->throw("Type $type is neither to or from coordinate systems");
+ throw("Type $type is neither to or from coordinate systems");
}
my @list;
@@ -541,11 +579,83 @@ sub _sort{
@{$self->{"_pair_$to"}->{$id}} = sort { $a->{'to'}->{'start'} <=> $b->{'to'}->{'start'} } @{$self->{"_pair_$to"}->{$id}};
}
+ $self->_merge_pairs();
+
$self->_is_sorted(1);
}
+# this function merges pairs that are adjacent into one
+sub _merge_pairs {
+ my $self = shift;
+
+ my ( $lr, $lr_from, $del_pair, $next_pair, $current_pair );
+
+ my $map_to = $self->{'to'};
+ my $map_from = $self->{'from'};
+ $self->{'pair_count'} = 0;
+
+ for my $key ( keys %{$self->{"_pair_$map_to"}} ) {
+ $lr = $self->{"_pair_$map_to"}->{$key};
+
+ my $i = 0;
+ my $next = 1;
+ my $length = $#$lr;
+ while( $next <= $length ) {
+ $current_pair = $lr->[$i];
+ $next_pair = $lr->[$next];
+ $del_pair = undef;
+
+ # duplicate filter
+ if( $current_pair->{'to'}->{'start'} == $next_pair->{'to'}->{'start'} ) {
+ $del_pair = $next_pair;
+ } elsif(( $current_pair->{'from'}->{'id'} eq $next_pair->{'from'}->{'id'} ) &&
+ ( $next_pair->{'ori'} == $current_pair->{'ori'} ) &&
+ ( $next_pair->{'to'}->{'start'} -1 == $current_pair->{'to'}->{'end'} )) {
+
+ if( $current_pair->{'ori'} == 1 ) {
+ # check forward strand merge
+ if( $next_pair->{'from'}->{'start'} - 1 == $current_pair->{'from'}->{'end'} ) {
+ # normal merge with previous element
+ $current_pair->{'to'}->{'end'} = $next_pair->{'to'}->{'end'};
+ $current_pair->{'from'}->{'end'} = $next_pair->{'from'}->{'end'};
+ $del_pair = $next_pair;
+ }
+ } else {
+ # check backward strand merge
+ if( $next_pair->{'from'}->{'end'} + 1 == $current_pair->{'from'}->{'start'} ) {
+ # yes its a merge
+ $current_pair->{'to'}->{'end'} = $next_pair->{'to'}->{'end'};
+ $current_pair->{'from'}->{'start'} = $next_pair->{'from'}->{'start'};
+ $del_pair = $next_pair;
+ }
+ }
+ }
+
+ if( defined $del_pair ) {
+ splice( @$lr, $next, 1 );
+ $lr_from = $self->{"_pair_$map_from"}->{uc($del_pair->{'from'}->{'id'})};
+ for( my $j=0; $j <= $#$lr_from; $j++ ) {
+ if( $lr_from->[$j] == $del_pair ) {
+ splice( @$lr_from, $j, 1 );
+ last;
+ }
+ }
+ $length--;
+ if( $length < $next ) { last; }
+ } else {
+ $next++;
+ $i++;
+ }
+ }
+ $self->{'pair_count'} += scalar( @$lr );
+ }
+}
+
+
+
+
=head2 _is_sorted
Arg 1 int $sorted
@@ -559,14 +669,8 @@ sub _sort{
sub _is_sorted{
my ($self,$value) = @_;
- if( defined $value) {
- $self->{'_is_sorted'} = $value;
- }
- if (! defined $self->{'_is_sorted'}) {
- $self->{'_is_sorted'} = 0;
- }
- return $self->{'_is_sorted'};
-
+ $self->{'_is_sorted'} = shift if(@_);
+ return $self->{'_is_sorted'};
}
diff --git a/modules/Bio/EnsEMBL/Mapper/Coordinate.pm b/modules/Bio/EnsEMBL/Mapper/Coordinate.pm
index 0aa1251249..acbbc3d627 100644
--- a/modules/Bio/EnsEMBL/Mapper/Coordinate.pm
+++ b/modules/Bio/EnsEMBL/Mapper/Coordinate.pm
@@ -146,4 +146,9 @@ sub id{
}
+sub length {
+ my $self = shift;
+ return $self->{'end'} - $self->{'start'} + 1;
+}
+
1;
diff --git a/modules/Bio/EnsEMBL/Mapper/Gap.pm b/modules/Bio/EnsEMBL/Mapper/Gap.pm
index 7775ee827a..6b475eb168 100644
--- a/modules/Bio/EnsEMBL/Mapper/Gap.pm
+++ b/modules/Bio/EnsEMBL/Mapper/Gap.pm
@@ -100,4 +100,11 @@ sub end{
}
+sub length {
+ my $self = shift;
+ return $self->{'end'} - $self->{'start'} + 1;
+}
+
+
+
1;
diff --git a/modules/Bio/EnsEMBL/Mapper/RangeRegistry.pm b/modules/Bio/EnsEMBL/Mapper/RangeRegistry.pm
new file mode 100644
index 0000000000..37b81e8ec4
--- /dev/null
+++ b/modules/Bio/EnsEMBL/Mapper/RangeRegistry.pm
@@ -0,0 +1,268 @@
+#
+# Ensembl module for Bio::EnsEMBL::Mapper::RangeRegistry
+#
+#
+# You may distribute this module under the same terms as perl itself
+
+=head1 NAME
+
+Bio::EnsEMBL::Mapper::RangeRegistry
+
+=head1 SYNOPSIS
+
+ use Bio::EnsEMBL::Mapper::RangeRegistry;
+
+ $rr = Bio::EnsEMBL::Mapper::RangeRegistry->new();
+
+ #get a fixed width chunk around the range of intereset
+ #this will be used if any registration is actually necessary
+ $chunk_start = ($start >> 20) << 20 + 1;
+ $chunk_end = (($end >> 20) + 1) << 20;
+
+ #check if any registration is necessary for the range.
+ #if it is register a large chunked area instead and return a listref
+ #of unregistered areas that need to be loaded.
+ if($pairs=$rr->check_and_register($id,$start,$end,$chunk_start,$chunk_end)) {
+ foreach my $pair (@$pairs) {
+ my ($pair_start, $pair_end) = @$pair;
+ #fetch mappings for these regions from the assembly table and load them
+ #into the mapper
+ ...
+ }
+ } else {
+ #the range ($start - $end) is already registered
+ ...
+ }
+
+
+ #check if any registration is necessary
+ #if it is register the region and return a listref of pairs that
+ #need to be loaded.
+ if($pairs = $rr->check_and_register($id, $start,$end)) {
+ }
+
+
+=head1 DESCRIPTION
+
+This module maintains an internal list of registered regions and is used to
+quickly ascertain if and what regions of a provided range need registration.
+
+=head1 AUTHOR - Graham McVicker
+
+=head1 CONTACT
+
+This modules is part of the Ensembl project http://www.ensembl.org
+
+Questions can be posted to the ensembl-dev mailing list:
+ensembl-dev@ebi.ac.uk
+
+=cut
+
+use strict;
+
+package Bio::EnsEMBL::Mapper::RangeRegistry;
+
+use Bio::EnsEMBL::Utils::Exception qw(throw);
+use integer;
+
+=head2 new
+
+ Arg [1] : none
+ Example : my $rr = Bio::EnsEMBL::Mapper::RangeRegistry->new();
+ Description: Creates a new RangeRegistry object
+ Returntype : Bio::EnsEMBL::Mapper::RangeRegistry
+ Exceptions : none
+ Caller : AssemblyMapperAdaptor
+
+=cut
+
+sub new {
+ my $caller = shift;
+ my $class = ref($caller) || $caller;
+ return bless {'registry' => {}}, $class;
+}
+
+
+sub flush {
+ my $self = shift;
+ $self->{'registry'} = {};
+}
+
+
+
+
+=head2 check_and_register
+
+ Arg [1] : string $id
+ The id of the range to be checked/registered (e.g. a sequenceid)
+ Arg [2] : int $start
+ The start of the range to be checked
+ Arg [3] : int $end
+ The end of the range to be checked
+ Arg [4] : (optional) int $rstart
+ The start of the range to be registered
+ if the checked range was not fully registered
+ Arg [5] : (optional) int $rend
+ The end of the range to be registerd
+ if the checked range was not fully registered
+ Example : $ranges=$rr->check_and_register('X',500,600, 1,1_000_000));
+ Description: Checks the range registry to see if the entire range
+ denoted by ($id : $start-$end) is already registered. If
+ it already is, then undef is returned. If it is not then
+ the range specified by $rstart and $rend is registered, and
+ a list of regions that were required to completely register
+ $rstart-$rend is returned. If $rstart and $rend are not
+ defined they default to $start and $end respectively.
+
+ The reason there is a single call to do both the checking and
+ registering is to reduce the overhead. Much of the work to
+ check if a range is registered is the same as registering a
+ region around that range.
+ Returntype : undef or listref of [start,end] range pairs
+ Exceptions : throw if rstart is greater than start
+ throw if rend is less than end
+ throw if end is less than start
+ throw if id, start, or end are not defined
+ Caller : AssemblyMapperAdaptor
+
+=cut
+
+#"constants"
+my $START = 0;
+my $END = 1;
+
+sub check_and_register {
+ my ($self, $id, $start, $end, $rstart,$rend) = @_;
+
+ $rstart = $start if(!defined($rstart));
+ $rend = $end if(!defined($rend));
+
+ #
+ # Sanity checks
+ #
+ if(!defined($id) || !defined($start) || !defined($end)) {
+ throw("ID, start, end arguments are required");
+ }
+
+ if($start > $end) {
+ throw("start argument must be less than end argument");
+ }
+
+ if($rstart >$rend) {
+ throw("rend [$rstart] argument must be less than rend [$rend] argument");
+ }
+
+ if($rstart > $start) {
+ throw("rstart must be less than or equal to start");
+ }
+
+ if($rend < $end) {
+ throw("rend must be greater than or equal to end");
+ }
+
+ my $reg = $self->{'registry'};
+ my $list = $reg->{$id} ||= [];
+
+ my @gap_pairs;
+
+ my $len = scalar(@$list);
+
+ if($len == 0) {
+ #this is the first request for this id, return a gap pair for the
+ #entire range and register it as seen
+ $list->[0] = [$rstart,$rend];
+ return [[$rstart,$rend]];
+ }
+
+ my $CUR;
+
+ #####
+ #loop through the list of existing ranges recording any "gaps" where the
+ #existing range does not cover part of the requested range
+ #
+ my $start_idx = 0;
+ my $end_idx = $#$list;
+ my ( $mid_idx, $range );
+
+ # binary search the relevant pairs
+ # helps if the list is big
+ while(( $end_idx - $start_idx ) > 1 ) {
+ $mid_idx = ($start_idx+$end_idx)>>1;
+ $range = $list->[$mid_idx];
+ if( $range->[1] < $start ) {
+ $start_idx = $mid_idx;
+ } else {
+ $end_idx = $mid_idx;
+ }
+ }
+
+
+ my ($gap_start, $gap_end, $r_idx, $rstart_idx, $rend_idx);
+ $gap_start = $rstart;
+
+ for($CUR=$start_idx; $CUR < $len; $CUR++) {
+ my ($pstart,$pend) = @{$list->[$CUR]};
+
+ #no work needs to be done at all if
+ #if we find a range pair that entirely overlaps the requested region
+ if($pstart <= $start && $pend >= $end) {
+ return undef;
+ }
+
+ #find adjacent or overlapping regions already registered
+ if($pend >= ($rstart-1) && $pstart <= ($rend+1)) {
+ if(!defined($rstart_idx)) {
+ $rstart_idx = $CUR;
+ }
+ $rend_idx = $CUR;
+ }
+
+ if($pstart > $rstart) {
+ $gap_end = ($rend < $pstart) ? $rend : $pstart-1;
+ push @gap_pairs, [$gap_start, $gap_end];
+ }
+
+ $gap_start = ($rstart > $pend) ? $rstart : $pend+1;
+
+ if($pstart > $rend && !defined($r_idx)) {
+ $r_idx = $CUR;
+ last;
+ }
+ }
+
+ #do we have to make another gap?
+ if($gap_start <= $rend) {
+ push @gap_pairs, [$gap_start, $rend];
+ }
+
+ #
+ # Merge the new range into the registered list
+ #
+ if(defined($rstart_idx)) {
+ my ($new_start, $new_end);
+ if($rstart < $list->[$rstart_idx]->[0]) {
+ $new_start = $rstart;
+ } else {
+ $new_start = $list->[$rstart_idx]->[0];
+ }
+
+ if($rend > $list->[$rend_idx]->[1]) {
+ $new_end = $rend;
+ } else {
+ $new_end = $list->[$rend_idx]->[1];
+ }
+
+ splice(@$list, $rstart_idx, $rend_idx-$rstart_idx+1, [$new_start, $new_end]);
+ } elsif(defined($r_idx)) {
+ splice(@$list, $r_idx, 0, [$rstart, $rend]);
+ } else {
+ push(@$list, [$rstart,$rend]);
+ }
+
+ return \@gap_pairs;
+}
+
+
+
+1;
+
diff --git a/modules/Bio/EnsEMBL/MiscFeature.pm b/modules/Bio/EnsEMBL/MiscFeature.pm
new file mode 100644
index 0000000000..64ff9b9b46
--- /dev/null
+++ b/modules/Bio/EnsEMBL/MiscFeature.pm
@@ -0,0 +1,263 @@
+#
+# Ensembl module for Bio::EnsEMBL::MiscFeature
+#
+# Copyright (c) 2003 EnsEMBL
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::MiscFeature - A miscelaneous feature with arbitrary features and
+associations.
+
+=head1 SYNOPSIS
+
+ use Bio::EnsEMBL::MiscFeature;
+
+ my $mfeat = Bio::EnsEMBL::MiscFeature->new(-START => 1200,
+ -END => 100_000,
+ -STRAND => 1,
+ -SLICE => $slice);
+
+ # Can add attributes to the misc feature and associate with various sets
+ $clone_set->code('clone');
+ $tiling_path_set->core('tilingpath');
+
+ $mfeat->add_attribute('name', 'RLX12451');
+ $mfeat->add_attribute('version', '4');
+ $mfeat->add_attribute('synonym', 'AL42131.4');
+ $mfeat->add_attrubute('synonym', 'Z199311');
+ $mfeat->add_set($clone_set);
+ $mfeat->add_set($tiling_path_set);
+
+ my ($name) = $mfeat->get_attribute('name');
+ my @synonyms = $mfeat->get_attribute('synonym');
+
+ my @attrib_types = $mfeat->get_attribute_types();
+ foreach my $attrib_type (@attrib_types) {
+ print join(',', $mfeat->get_attribute($attrib_type));
+ }
+
+ $clone_set = $mfeat->get_set('clone');
+ $tiling_path_set = $mfeat->get_set('tiling_path');
+
+ my @set_codes = $mfeat->get_set_codes();
+ foreach my $set_code (@set_codes) {
+ print $mfeat->get_set->name(), "\n";
+ }
+
+
+ # Can do normal feature operations as well
+ $mfeat = $mfeat->transform('supercontig');
+ print $mfeat->slice->seq_region_name, ' ', $mfeat->start, '-', $mfeat->end;
+
+
+=head1 DESCRIPTION
+
+MiscFeatures are extremely general features with a location, and an
+arbitrary group of attributes. They are grouped with other features of the
+same 'type' through the use of MiscSets (see Bio::EnsEMBL::MiscSet).
+
+=head1 CONTACT
+
+This modules is part of the Ensembl project http://www.ensembl.org
+
+Questions can be posted to the ensembl-dev mailing list:
+ensembl-dev@ebi.ac.uk
+
+=cut
+
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::MiscFeature;
+
+use Bio::EnsEMBL::Feature;
+
+use Bio::EnsEMBL::Utils::Exception qw(throw);
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::Feature);
+
+
+# new is inherited from superclass
+
+sub new_fast {
+ my $class = shift;
+ my $hashref = shift;
+ return bless($hashref, $class);
+}
+
+
+
+
+=head2 add_attribute
+
+ Arg [1] : string $type
+ The type of attribute to add
+ Arg [2] : string $value
+ The value of the attribute to add
+ Example : $misc_feature->add_attribute('synonym', 'AL124141.1');
+ Description: Adds an attribute of a given type to this misc. feature
+ Returntype : none
+ Exceptions : throw if type arg is not provided
+ Caller : general
+
+=cut
+
+sub add_attribute {
+ my ($self, $type, $value) = @_;
+
+ throw('Type argument is required') if(!$type);
+
+ $self->{'attributes'}->{$type} ||= [];
+ push @{$self->{'attributes'}->{$type}}, $value
+}
+
+
+=head2 add_set
+
+ Arg [1] : Bio::EnsEMBL::MiscSet $set
+ The set to add
+ Example : $misc_feature->add_set(Bio::EnsEMBL::MiscSet->new(...));
+ Description: Associates this MiscFeature with a given Set.
+ Returntype : none
+ Exceptions : throw if the set arg is not provided,
+ throw if the set to be added does not have a code
+ Caller : general
+
+=cut
+
+sub add_set {
+ my ($self, $set) = @_;
+
+ if(!$set || !ref($set) || !$set->isa('Bio::EnsEMBL::MiscSet')) {
+ throw('Set argument must be a Bio::EnsEMBL::MiscSet');
+ }
+
+ my $code = $set->code();
+
+ throw('Set must be associated with a code to be added') if(!$code);
+
+ $self->{'sets'}->{$code} = $set;
+}
+
+
+
+=head2 get_attribute_types
+
+ Arg [1] : none
+ Example : @attrib_types = $misc_feature->get_attribute_types();
+ Description: returns a list of attribute types that this feature has
+ Returntype : list of strings
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub get_attribute_types {
+ my $self = shift;
+ $self->{'attributes'} ||= {};
+
+ return keys %{$self->{'attributes'}};
+}
+
+=head2 get_set_codes
+
+ Arg [1] : none
+ Example : @set_codes = $misc_feature->get_set_codes();
+ Description: returns a list of codes for the sets this feature is associated
+ with
+ Returntype : list of strings
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub get_set_codes {
+ my $self = shift;
+ $self->{'sets'} ||= {};
+ return keys %{$self->{'sets'}};
+}
+
+
+=head2 get_set
+
+ Arg [1] : string $code
+ The code of the set to retrieve
+ Example : $set = $misc_feature->get_set($code);
+ Description: Retrieves a set that this feature is associated with via its
+ code. Undef is returned if the feature is not associated with
+ the requested set.
+ Returntype : Bio::EnsEMBL::MiscSet or undef
+ Exceptions : throw if the code arg is not provided
+ Caller : general
+
+=cut
+
+sub get_set {
+ my $self = shift;
+ my $code = shift;
+
+ throw('Code arg is required.') if (!$code);
+
+ return $self->{'sets'}->{$code};
+}
+
+
+
+=head2 get_attribute
+
+ Arg [1] : string $type
+ The type of the attribute values to retrieve
+ Example : @values = $misc_feature->get_attribute('name');
+ Description: Retrieves a list of values for a particular attribute type
+ Returntype : list of strings
+ Exceptions : thrown if a type arg is not provided
+ Caller : general
+
+=cut
+
+sub get_attribute {
+ my $self = shift;
+ my $type = shift;
+
+ throw('Type arg is required.') if(!$type);
+
+ if(exists $self->{'attributes'}->{$type}) {
+ return @{$self->{'attributes'}->{$type}};
+ }
+
+ return ();
+}
+
+
+
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $kb->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For misc_features this is the first
+ name or synonym attribute or '' if neither are defined.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
+
+=cut
+
+sub display_id {
+ my $self = shift;
+ my ($attrib) = $self->get_attribute('name');
+ ($attrib) = $self->get_attribute('synonym') if(!$attrib);
+ return $attrib || '';
+}
+
+
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/MiscSet.pm b/modules/Bio/EnsEMBL/MiscSet.pm
new file mode 100644
index 0000000000..47007e2002
--- /dev/null
+++ b/modules/Bio/EnsEMBL/MiscSet.pm
@@ -0,0 +1,178 @@
+#
+# Ensembl module for Bio::EnsEMBL::MiscSet
+#
+# Copyright (c) 2003 EnsEMBL
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::MiscSet - This is a set representing a classification of
+a group of miscellaneuos features.
+
+=head1 SYNOPSIS
+
+ use Bio::EnsEMBL::MiscSet;
+
+ ...
+
+ my $misc_set = Bio::EnsEMBL::MiscSet->new(1234, $adaptor,
+ 'tilepath',
+ 'Assembly Tiling Path',
+ 'The tiling path of clones',
+ 1e6);
+
+ my $misc_feature->add_set($misc_set);
+
+
+=head1 DESCRIPTION
+
+MiscSets represent classsifications or groupings of MiscFeatures. Features are
+classified into sets essentially to define what they are and how they may be
+used. Generally MiscFeatures are retrieved on the basis of their associated
+sets. See Bio::EnsEMBL::MiscFeature, Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor.
+
+Note that MiscSets and MiscFeatures were formerly known as MapSets and MapFrags
+
+=head1 CONTACT
+
+This modules is part of the Ensembl project http://www.ensembl.org
+
+Questions can be posted to the ensembl-dev mailing list:
+ensembl-dev@ebi.ac.uk
+
+=cut
+
+use strict;
+
+package Bio::EnsEMBL::MiscSet;
+
+use Bio::EnsEMBL::Storable;
+
+use vars qw(@ISA);
+
+@ISA = qw(Bio::EnsEMBL::Storable);
+
+
+
+
+=head2 new
+
+ Arg [1] : int $misc_set_id
+ The internal identifier for this misc set
+ Arg [2] : string $code
+ The unique code which identifies this set type
+ Arg [3] : string $name
+ The human readable name of this set
+ Arg [4] : string $desc
+ The description of this set
+ Arg [5] : int $max_len
+ The maximum length of features of this mapset
+ Example : $set = new Bio::EnsEMBL::MiscSet(1234, 'tilepath',
+ 'Assembly Tiling Path',
+ 'The tiling path of clones',
+ 1e6);
+ Description: Instantiates a Bio::EnsEMBL::MiscSet
+ Returntype : Bio::EnsEMBL::MiscSet
+ Exceptions : none
+ Caller : MiscFeatureAdaptor
+
+=cut
+
+sub new {
+ my ($caller, $misc_set_id, $adaptor, $code, $name, $desc, $max_len) = @_;
+
+ my $class = ref($caller) || $caller;
+
+ if($adaptor && !(ref($adaptor) ||
+ !$adaptor->isa('Bio::EnsEMBL::DBSQL::MiscSetAdaptor'))) {
+ throw('Adaptor arg must be a Bio::EnsEMBL::DBSQL::MiscSetAdaptor');
+ }
+
+ return bless {'dbID' => $misc_set_id,
+ 'adaptor' => $adaptor,
+ 'code' => $code,
+ 'name' => $name,
+ 'description' => $desc,
+ 'longest_feature' => $max_len}, $class;
+}
+
+=head2 code
+
+ Arg [1] : string $newval (optional)
+ The new value to set the code attribute to
+ Example : $code = $obj->code()
+ Description: Getter/Setter for the code attribute
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub code{
+ my $self = shift;
+ $self->{'code'} = shift if(@_);
+ return $self->{'code'};
+}
+
+
+=head2 name
+
+ Arg [1] : string $newval (optional)
+ The new value to set the code attribute to
+ Example : $name = $obj->name()
+ Description: Getter/Setter for the name attribute
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub name {
+ my $self = shift;
+ $self->{'name'} = shift if(@_);
+ return $self->{'name'};
+}
+
+
+=head2 description
+
+ Arg [1] : string $newval (optional)
+ The new value to set the description attribute to
+ Example : $description = $obj->description()
+ Description: Getter/Setter for the description attribute
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub description{
+ my $self = shift;
+ $self->{'description'} = shift if(@_);
+ return $self->{'description'};
+}
+
+
+=head2 longest_feature
+
+ Arg [1] : int $newval (optional)
+ The new value to set the longest_feature attribute to
+ Example : $longest_feature = $obj->longest_feature()
+ Description: Getter/Setter for the longest_feature attribute
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub longest_feature{
+ my $self = shift;
+ $self->{'longest_feature'} = shift if(@_);
+ return $self->{'longest_feature'};
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/PredictionExon.pm b/modules/Bio/EnsEMBL/PredictionExon.pm
new file mode 100644
index 0000000000..bfc9a3cc15
--- /dev/null
+++ b/modules/Bio/EnsEMBL/PredictionExon.pm
@@ -0,0 +1,291 @@
+#
+# EnsEMBL module for Bio::EnsEMBL::PredictionExon
+#
+#
+# Copyright Ewan Birney
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=pod
+
+=head1 NAME Bio::EnsEMBL::PredictionExon - A class representing an Exon from an
+ab initio prediction method
+
+=head1 SYNOPSIS
+
+ $ex = new Bio::EnsEMBL::Exon(-START => 100,
+ -END => 200,
+ -STRAND => 1,
+ -SLICE => $slice,
+ -DBID => $dbID,
+ -P_VALUE => 23.5,
+ -SCORE => 99
+ );
+
+ #seq returns a Bio::Seq
+ my $seq = $exon->seq->seq();
+
+ #peptide only makes sense within transcript context
+ my $pep = $exon->peptide($transcript)->seq();
+
+ #normal feature operations can be performed:
+ $exon = $exon->transform('clone');
+ $exon->move($new_start, $new_end, $new_strand);
+ print $exon->slice->seq_region_name();
+
+=head1 DESCRIPTION
+
+This is a class which represents an prediction exon which is part of a
+predcition transcript. See Bio::EnsEMBL:PredictionTranscript
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL developer list: <ensembl-dev@ebi.ac.uk>
+
+=head1 APPENDIX
+
+The rest of the documentation details each of the object methods. Internal
+methods are usually preceded with a_
+
+=cut
+
+package Bio::EnsEMBL::PredictionExon;
+use vars qw(@ISA);
+use strict;
+
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Exon;
+use Bio::EnsEMBL::Utils::Exception qw( warning throw deprecate );
+use Bio::EnsEMBL::Utils::Argument qw( rearrange );
+
+
+@ISA = qw(Bio::EnsEMBL::Exon);
+
+
+=head2 new
+
+ Args : see SUPERCLASS Bio::EnsEMBL::Exon
+ Example : none
+ Description: create an Exon object
+ Returntype : Bio::EnsEMBL::Exon
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub new {
+ my $class = shift;
+
+ $class = ref $class || $class;
+
+ my $self = $class->SUPER::new( @_ );
+
+ my ( $p_value, $score ) =
+ rearrange( [ "P_VALUE", "SCORE" ], @_ );
+
+ $self->{'p_value'} = $p_value;
+ $self->{'score'} = $score;
+
+ return $self;
+}
+
+
+=head2 score
+
+ Arg [1] : string $newval (optional)
+ The new value to set the score attribute to
+ Example : $score = $obj->score()
+ Description: Getter/Setter for the score attribute
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub score{
+ my $self = shift;
+ $self->{'score'} = shift if(@_);
+ return $self->{'score'};
+}
+
+
+
+=head2 p_value
+
+ Arg [1] : string $newval (optional)
+ The new value to set the p_value attribute to
+ Example : $p_value = $obj->p_value()
+ Description: Getter/Setter for the p_value attribute
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub p_value{
+ my $self = shift;
+ $self->{'p_value'} = shift if(@_);
+ return $self->{'p_value'};
+}
+
+
+=head2 end_phase
+
+ Arg [1] : (optional) int $end_phase
+ Example : $end_phase = $feat->end_phase;
+ Description: Gets/Sets the end phase of the exon.
+ end_phase = number of bases from the last incomplete codon of
+ this exon.
+ Usually, end_phase = (phase + exon_length)%3
+ but end_phase could be -1 if the exon is half-coding and its 3
+ prime end is UTR.
+ Returntype : int
+ Exceptions : warning if end_phase is called without an argument and the
+ value is not set.
+ Caller : general
+
+=cut
+
+
+
+sub end_phase {
+ my $self = shift;
+ if( @_ ) {
+ throw( "End_phase setting not supported" );
+ }
+ return (3- $self->phase() + $self->length()) % 3;
+}
+
+
+=head2 transform
+
+ Arg 1 : String $coordinate_system_name
+ Arg [2] : String $coordinate_system_version
+ Description: moves this exon to the given coordinate system. If this exon has
+ attached supporting evidence, they move as well.
+ Returntype : Bio::EnsEMBL::Exon
+ Exceptions : wrong parameters
+ Caller : general
+
+=cut
+
+
+sub transform {
+ my $self = shift;
+
+ # catch for old style transform calls
+ if( !@_ || ( ref $_[0] && $_[0]->isa( "Bio::EnsEMBL::Slice" ))) {
+ throw( "transform needs coordinate systems details now," .
+ "please use transfer" );
+ }
+
+ my $new_exon = Bio::EnsEMBL::Feature::transform( $self, @_ );
+ return undef unless $new_exon;
+
+ #dont want to share the same sequence cache
+ delete $new_exon->{'_seq_cache'};
+
+ return $new_exon;
+}
+
+
+
+=head2 transfer
+
+ Arg [1] : Bio::EnsEMBL::Slice $destination_slice
+ Example : none
+ Description: Moves this Exon to given target slice coordinates. If Features
+ are attached they are moved as well. Returns a new exon.
+ Returntype : Bio::EnsEMBL::Gene
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub transfer {
+ my $self = shift;
+
+ my $new_exon = Bio::EnsEMBL::Feature::transfer( $self, @_ );
+ return undef unless $new_exon;
+
+ #dont want to share the same sequence cache
+ delete $new_exon->{'_seq_cache'};
+
+ return $new_exon;
+}
+
+
+
+
+
+
+=head2 add_supporting_features
+
+ Arg [1] : Bio::EnsEMBL::SeqFeatureI $feature
+ Example : $exon->add_supporting_features(@features);
+ Description: Adds a list of supporting features to this exon.
+ Duplicate features are not added.
+ If supporting features are added manually in this
+ way, prior to calling get_all_supporting_features then the
+ get_all_supporting_features call will not retrieve supporting
+ features from the database.
+ Returntype : none
+ Exceptions : throw if any of the features are not SeqFeatureIs
+ throw if any of the features are not in the same coordinate
+ system as the exon
+ Caller : general
+
+=cut
+
+sub add_supporting_features {
+ throw( "This object doesnt have supporting_feature" );
+}
+
+
+=head2 get_all_supporting_features
+
+ Arg [1] : none
+ Example : @evidence = @{$exon->get_all_supporting_features()};
+ Description: Retreives any supporting features added manually by
+ calls to add_supporting_features. If no features have been
+ added manually and this exon is in a database (i.e. it h
+ Returntype : listreference of Bio::EnsEMBL::BaseAlignFeature objects
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub get_all_supporting_features {
+ throw( "This object doesnt have supporting_feature" );
+}
+
+
+=head2 find_supporting_evidence
+
+ Arg [1] : Bio::EnsEMBL::SeqFeatureI $features
+ The list of features to search for supporting (i.e. overlapping)
+ evidence.
+ Arg [2] : (optional) boolean $sorted
+ Used to speed up the calculation of overlapping features.
+ Should be set to true if the list of features is sorted in
+ ascending order on their start coordinates.
+ Example : $exon->find_supporting_evidence(\@features);
+ Description: Looks through all the similarity features and
+ stores as supporting features any feature
+ that overlaps with an exon.
+ Returntype : none
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub find_supporting_evidence {
+ throw( "This object doesnt have supporting_feature" );
+}
+
+
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/PredictionTranscript.pm b/modules/Bio/EnsEMBL/PredictionTranscript.pm
index 767a181f45..54d0035cc5 100644
--- a/modules/Bio/EnsEMBL/PredictionTranscript.pm
+++ b/modules/Bio/EnsEMBL/PredictionTranscript.pm
@@ -3,7 +3,6 @@
#
# Cared for by Arne Stabenau
#
-
#
# You may distribute this module under the same terms as perl itself
@@ -18,10 +17,9 @@ PredictionTranscript
=head1 DESCRIPTION
-Container for single transcript ab initio gene prediction ala GenScan.
+Container for single transcript ab initio gene prediction ala GenScan or SNAP.
Is directly storable/retrievable in EnsEMBL using PredictionTranscript Adaptor.
-
Creation:
my $tran = new Bio::EnsEMBL::PredictionTranscript();
@@ -54,288 +52,66 @@ contact EnsEMBL dev <ensembl-dev@ebi.ac.uk> for information
=cut
-
-# Let the code begin...
-
package Bio::EnsEMBL::PredictionTranscript;
use vars qw(@ISA);
use strict;
-
-use Bio::EnsEMBL::Root;
-use Bio::EnsEMBL::Exon;
+use Bio::EnsEMBL::Feature;
use Bio::EnsEMBL::Transcript;
-use Bio::Seq;
-
-@ISA = qw(Bio::EnsEMBL::Root Bio::EnsEMBL::TranscriptI);
-
-=head2 new
-
- Arg [1-] : Bio::EnsEMBL::Exon $exon
- Exons which make up this transcript in the right order.
- Optional, can be added as well with add_Exon.
- Function : Creates a new PredictionTranscript
- Returntype: Bio::EnsEMBL::PredictionTranscript
- Exceptions: none
- Caller : Adaptor, Genscan Runnable
-
-=cut
-
-sub new {
- my($class,@optional_exons) = @_;
-
- if( ref $class ) {
- $class = ref $class;
- }
-
- my $self = {};
- bless $self,$class;
-
- # set stuff in self from @args
- foreach my $exon (@optional_exons) {
- if( ! defined $self->{'exons'} ) {
- $self->{'exons'} = [];
- }
-
- $self->add_Exon($exon);
- }
-
- return $self;
-}
-
-
-
-=head2 stable_id
-
- Arg [1] : (optional) $stable_id
- Example : my $pt_id = $prediction_transcript->stable_id;
- Description: Retrieves the stable id fro this prediction transcript.
- Prediction transcripts do not maintain stable ids as real
- transcripts do - the id is constructed from the name of the
- contig the prediction transcript was pulled off of, and the
- start and end of the transcript on the contig.
- i.e. the stable id is: "$contig_name.$contig_start.$contig_end"
- Returntype : string
- Exceptions : none
- Caller : general, PredictionTranscriptAdaptor
-
-=cut
-
-sub stable_id {
- my ($self, $value) = @_;
+use Bio::EnsEMBL::Translation;
- if($value) {
- $self->{'_stable_id'} = $value;
- }
+use Bio::EnsEMBL::Utils::Exception qw( deprecate throw warning );
+use Bio::EnsEMBL::Utils::Argument qw( rearrange );
- return $self->{'_stable_id'};
-}
+@ISA = qw(Bio::EnsEMBL::Transcript);
=head2 coding_region_start
- Arg [1] : The new coding start of this prediction transcript in slice
- coords.
- Function : Getter/Setter for the coding start of this transcript.
- Implemented to satisfy requirements of TranscriptI interface
- and so that it can be drawn as a Transcript. Since prediction
- transcripts do not currently have UTRs the coding start should
- return the same value as the start method.
- By convention, the coding_region_start is always lower than the
- value returned by the coding_region_end method. The value
- returned by this function is NOT the biological coding start
- since on the reverse strand the biological coding start would
- be the higher genomic value.
- Returntype: scalar int
- Exceptions: none
- Caller : GlyphSet_transcript
+ Arg [1] : none
+ Example : $coding_region_start = $pt->coding_region_start
+ Description: Retrieves the start of the coding region of this transcript in
+ slice coordinates. For prediction transcripts this
+ is always the start of the transcript (i.e. there is no UTR).
+ By convention, the coding_region_start is always lower than
+ the value returned by the coding_end method.
+ The value returned by this function is NOT the biological
+ coding start since on the reverse strand the biological coding
+ start would be the higher genomic value.
+ Returntype : int
+ Exceptions : none
+ Caller : general
=cut
sub coding_region_start {
- my ($self, $arg) = @_;
-
- if(defined $arg) {
- $self->{'coding_region_start'} = $arg;
- } elsif(!defined $self->{'coding_region_start'}) {
- #if the coding start is not defined, use the start of the transcript
- return $self->start();
- }
-
- return $self->{'coding_region_start'};
+ my $self = shift;
+ return $self->start();
}
=head2 coding_region_end
- Arg [1] : (optional) The new coding end of this prediction transcript
- in slice coords.
- Function : Getter/Setter for the coding end of this transcript.
- Implemented to satisfy requirements of TranscriptI interface
- and so that it can be drawn as a Transcript. Since prediction
- transcripts do not currently have UTRs the coding end should
- be the same as the end of the transcript.
- By convention, the coding_region_start is always lower than the
- value returned by the coding_region_end method. The value
- returned by this function is NOT the biological coding start
- since on the reverse strand the biological coding start would
- be the higher genomic value.
- Returntype: scalar int
- Exceptions: none
- Caller : GlyphSet_transcript
+ Arg [1] : none
+ Example : $coding_region_end = $transcript->coding_region_end
+ Description: Retrieves the start of the coding region of this prediction
+ transcript. For prediction transcripts this is always the same
+ as the end since no UTRs are stored.
+ By convention, the coding_region_end is always higher than the
+ value returned by the coding_region_start method.
+ The value returned by this function is NOT the biological
+ coding start since on the reverse strand the biological coding
+ end would be the lower genomic value.
+ Returntype : int
+ Exceptions : none
+ Caller : general
=cut
sub coding_region_end {
- my ($self, $arg) = @_;
-
- if(defined $arg) {
- $self->{'coding_region_end'} = $arg;
- } elsif(!defined $self->{'coding_region_end'}) {
- #if the coding end is not defined, use the end of the transcript
- return $self->end();
- }
- return $self->{'coding_region_end'};
-}
-
-
-=head2 start
-
- Arg [1] : The new start of this prediction transcript in slice
- coords.
- Function : Getter/Setter for the start of this transcript.
- Implemented to satisfy requirements of TranscriptI interface
- and so that it can be drawn as a Transcript.
- Returntype: scalar int
- Exceptions: none
- Caller : GlyphSet_transcript
-
-=cut
-
-sub start {
- my ($self, $arg) = @_;
-
- if(defined $arg) {
- $self->{'start'} = $arg;
- }
-
- return $self->{'start'};
-}
-
-=head2 end
-
- Arg [1] : The new end of this prediction transcript in slice coords.
- Function : Getter/Setter for the end of this transcript.
- Implemented to satisfy requirements of TranscriptI interface
- and so that it can be drawn as a Transcript.
- Returntype: scalar int
- Exceptions: none
- Caller : GlyphSet_transcript
-
-=cut
-
-sub end {
- my ($self, $arg) = @_;
-
- if(defined $arg) {
- $self->{'end'} = $arg;
- }
-
- return $self->{'end'};
-}
-
-
-## Attribute section ##
-
-sub analysis {
- my ( $self, $value ) = @_;
- ( defined $value ) &&
- ( $self->{'analysis'} = $value );
- return $self->{'analysis'};
-}
-
-
-sub dbID {
- my $self = shift;
-
- if( @_ ) {
- my $value = shift;
- $self->{'dbID'} = $value;
- }
- return $self->{'dbID'};
-
-}
-
-sub adaptor {
- my $self = shift;
-
- if( @_ ) {
- my $value = shift;
- $self->{'adaptor'} = $value;
- }
- return $self->{'adaptor'};
-}
-
-## end attribute section ##
-
-=head2 add_Exon
-
- Arg 1 : Bio::EnsEMBL::Exon $exon
- Arg [2] : int $exon_position
- Use it when you know you dont have exons in the
- beginning. Useful for the Adaptor when retrieving
- partial PT..
- Function : Adds given Exon to this prediction transcript.
- It can be at arbitrary position in the array. Not filled lower
- positions in the exon list are set undef then. Counting starts at 1.
- Returntype: none
- Exceptions: if argument is not Bio::EnsEMBL::Exon
- Caller : Pipeline runnable Genscan
-
-=cut
-
-
-sub add_Exon {
- my ($self, $exon, $position) = @_;
- if( defined $position ) {
- $self->{'exons'}[$position-1] = $exon;
- } else {
- push( @{$self->{'exons'}}, $exon );
- }
-
- if(defined $exon && (!defined $self->{'start'} ||
- $exon->start() < $self->{'start'})) {
- $self->start($exon->start());
- }
- if(defined $exon && (!defined $self->{'end'} ||
- $exon->end() > $self->{'end'})) {
- $self->end($exon->end());
- }
-}
-
-
-
-=head2 get_all_Exons
-
- Arg [1] : optional 1 $wish_undefined_exons
- Function : Returns all Exons currently in the PredictionTranscript
- in the order 5' to 3'. If this is a partial PredictionTranscript,
- elements of the list will be undef.
- Returntype: listref Bio::EnsEMBL::Exon
- Exceptions: none
- Caller : self->get_cdna(),Web for display.
-
-=cut
-
-sub get_all_Exons {
- my ($self, $wish_undefined_exon ) = @_;
-
- if( $wish_undefined_exon ) {
- return $self->{'exons'};
- } else {
- return [ grep{ ref( $_ ) eq 'Bio::EnsEMBL::Exon' } @{$self->{'exons'}} ];
- }
+ my $self = shift;
+ return $self->end();
}
@@ -344,12 +120,10 @@ sub get_all_Exons {
Arg [1] : none
Example : $exons = $self->get_all_translateable_Exons
- Description: Retreives the same value of get_all_Exons for this prediction
- transcript with the exception that undefined exons (only when
- transcript is in slice coords and exon maps to gap) are not
- returned. In a prediction transcript there is no UTR and
- thus all exons are entirely translateable.
- Returntype : listref of Bio::EnsEMBL::Exon
+ Description: Retrieves the translateable portion of all exons in this
+ transcript. For prediction transcripts this means all exons
+ since no UTRs are stored for them.
+ Returntype : listref of Bio::EnsEMBL::PredictionExons
Exceptions : none
Caller : general
@@ -357,154 +131,113 @@ sub get_all_Exons {
sub get_all_translateable_Exons {
my $self = shift;
-
- return [ grep{ ref( $_ ) eq 'Bio::EnsEMBL::Exon' } @{$self->get_all_Exons(1)} ];
+ return $self->get_all_Exons();
}
-=head2 sort
+=head2 stable_id
- Function: Sorts the exons by start coordinate
- Sorts forward for forward strand and reverse for reverse strand
- It refills $self->{'exons'} with the sorted exons
- Returns : none
- Args : none
+ Arg [1] : none
+ Example : print $pt->stable_id();
+ Description: Gets a 'stable' identifier for this prediction transcript. Note
+ that prediction transcripts do not have real stable
+ identifiers (i.e. identifiers maintained between releases and
+ stored in the database) and this method is provided to be
+ polymorphic with the Transcript class.
+ The stable identifer returned returned is formed by concating
+ the logic-name of the prediction transcripts analysis with
+ the transcripts dbID (0 Left padded to 11digits).
+ Returntype : string
+ Exceptions :
+ Caller :
=cut
-sub sort {
+sub stable_id {
my $self = shift;
- # dont sort if there are undefined exons
- if( grep { ! defined $_ } @{$self->{'exons'}} ) {
- return;
- }
- # Fetch all the exons
- my @exons = @{$self->get_all_Exons()};
+ my $id = $self->dbID();
+ return '' if(!$id);
- # Empty the exon holder
- $self->flush_Exons();
+ my $analysis = $self->analysis();
+ my $logic_name = uc($analysis->logic_name()) if($analysis);
+ $logic_name ||= 'PTRANS';
- # Now sort the exons and put back in the feature table
- my $strand = $exons[0]->strand;
-
- if ($strand == 1) {
- @exons = sort { $a->start <=> $b->start } @exons;
- }
- elsif ($strand == -1) {
- @exons = sort { $b->start <=> $a->start } @exons;
- }
+ my $pad = 11;
+ $pad -= length($id);
- foreach my $e (@exons) {
- $self->add_Exon($e);
- }
+ return $logic_name . ('0' x $pad) . $id;
}
+sub get_all_DBEntries { return []; }
-=head2 get_exon_count
+sub get_all_DBLinks { return []; }
- Args : none
- Function : How many exons are in this PTranscript. Some might
- not be in this object, depending on how it was retrieved.
- (non golden exons missing on Slice->get_predicitonTranscripts())
- Returntype: int
- Exceptions: none
- Caller : general
+sub add_DBEntry {}
-=cut
+sub external_db { return undef; }
+sub external_status { return undef; }
-sub get_exon_count {
- my $self = shift;
- return scalar( @{$self->{'exons'}} );
-}
+sub external_name { return undef; }
+sub is_known { return 0;}
-=head2 set_exon_count
+=head2 translation
- Arg 1 : int $number_of_exons
- If the number of exons you put in with add_exon is not the
- real number of exons in the Transcript, you can set it here.
- Might be necessary in db gets, where you dont get all.
- Function : sets number of exons, so get_all_Exons returns more undef exons
- at the end. After this, you have to use position argument when you
- want to insert Exons.
- Returntype: none
- Exceptions: If you set less then already in, you loose what you have :)
- Caller : $self->adaptor()
+ Arg [1] : none
+ Example : $translation = $pt->translation();
+ Description: Retrieves a Bio::EnsEMBL::Translation object for this prediction
+ transcript. Note that this translation is generated on the fly
+ and is not stored in the database. The translation always
+ spans the entire transcript (no UTRs; all CDS) and does not
+ have an associated dbID, stable_id or adaptor.
+ Returntype : int
+ Exceptions : none
+ Caller : general
=cut
-sub set_exon_count {
+sub translation {
my $self = shift;
- my $number_of_exons = shift;
-
- if( ! defined $self->{'exons'} ) {
- $self->{'exons'} = [];
- }
-
- $#{$self->{'exons'}} = $number_of_exons-1;
-}
+ #calculate translation on the fly
+ my $strand = $self->strand();
+ my $start_exon;
+ my $end_exon;
-=head2 length
-
- Args : none
- Function : length of DNA of all Exons in the PT together. No phase padding
- is done.
- Returntype: int
- Exceptions: differs from length in get_cdna() as that might be padded.
- Caller : unknown
+ my @exons = @{$self->get_all_Exons()};
-=cut
+ return undef if(!@exons);
+ if($strand == 1) {
+ $start_exon = $exons[0];
+ $end_exon = $exons[-1];
+ } else {
+ $start_exon = $exons[-1];
+ $end_exon = $exons[0];
+ }
-sub length {
- my( $self ) = @_;
-
- my $length = 0;
- foreach my $ex (@{$self->get_all_Exons}) {
- if( defined $ex ) { $length += $ex->length };
- }
- return $length;
+ return
+ Bio::EnsEMBL::Translation->new(-START_EXON => $start_exon,
+ -END_EXON => $end_exon,
+ -SEQ_START => 1,
+ -SEQ_END => $end_exon->length());
}
-
-=head2 flush_Exons
-
- Args : none
- Function : Removes all Exons from this PT. Resets count to zero.
- Returntype: none
- Exceptions: none
- Caller : for completeness, use not likely.
-
-=cut
-
-sub flush_Exons {
- my ($self,@args) = @_;
-
- $self->{'exons'} = [];
- $self->{'start'} = undef;
- $self->{'end'} = undef;
- $self->{'coding_start'} = undef;
- $self->{'coding_end'} = undef;
-}
-
-
=head2 translate
Args : none
Function : Give a peptide translation of all exons currently in
the PT. Gives empty string when none is in.
Returntype: a Bio::Seq as in transcript->translate()
- Exceptions: if the exons come in two or more groups, with an undef exon
- in the middle, only the first group is translated.
+ Exceptions: none
Caller : general
=cut
@@ -513,7 +246,7 @@ sub flush_Exons {
sub translate {
my ($self) = @_;
- my $dna = $self->get_cdna();
+ my $dna = $self->translateable_seq();
$dna =~ s/TAG$|TGA$|TAA$//i;
# the above line will remove the final stop codon from the mrna
# sequence produced if it is present, this is so any peptide produced
@@ -522,322 +255,203 @@ sub translate {
# or call translatable seq directly and produce a translation from it
my $bioseq = new Bio::Seq( -seq => $dna, -moltype => 'dna' );
-
+
return $bioseq->translate();
}
-
-=head2 get_cdna
+=head2 cdna_coding_start
- Args : none
- Function : Give a concat cdna of all exons currently in
- the PT. Gives empty string when none is in. Pads between not
- phase matching exons. Builds internal coord translation table.
- Returntype: txt
- Exceptions: if the exons come in two or more groups, with an undef exon
- in the middle, only the first groups cdna is returned.
- Caller : general, $self->translate()
+ Arg [1] : none
+ Example : $relative_coding_start = $transcript->cdna_coding_start;
+ Description: Retrieves the position of the coding start of this transcript
+ in cdna coordinates (relative to the start of the 5prime end of
+ the transcript, excluding introns, including utrs). This is
+ always 1 for prediction transcripts because they have no UTRs.
+ Returntype : int
+ Exceptions : none
+ Caller : five_prime_utr, get_all_snps, general
=cut
-sub get_cdna {
- my $self = shift;
-
- my $exons = $self->get_all_Exons();
-
- my $cdna = undef;
- my $lastphase = 0;
-
- my ( $cdna_start, $cdna_end );
- my ( $pep_start, $pep_end );
- my ( $new_cdna, $pep_count );
-
- $cdna_start = 1;
- $pep_start = 1;
+sub cdna_coding_start { return 1; }
- $self->{'_exon_align'} = [];
- for my $exon ( @$exons ) {
- my $exon_align = {};
- if( ! defined $exon ) {
- if( ! defined $cdna ) {
- next;
- } else {
- last;
- }
- }
- push( @{$self->{'_exon_align'}}, $exon_align );
+=head2 cdna_coding_end
- my $phase = 0;
-
- if (defined($exon->phase)) {
- $phase = $exon->phase;
- }
-
- if( $phase != $lastphase ) {
-
- if( $lastphase == 1 ) {
- $cdna .= 'NN';
- $cdna_start += 2;
- $pep_start++;
- } elsif( $lastphase == 2 ) {
- $cdna .= 'N';
- $cdna_start += 1;
- $pep_start++;
- }
-
- #startpadding for this exon
- $cdna .= 'N' x $phase;
- $cdna_start += $phase;
- }
-
- $new_cdna = $exon->seq->seq();
-# print $new_cdna."\n";
- $cdna .= $new_cdna;
- $cdna_end = $cdna_start + CORE::length( $new_cdna ) - 1;
-
- # how many peptides are added by this exon??
-
- $pep_count = int( ( CORE::length( $new_cdna ) + $phase + 2 ) / 3 );
-
- $pep_end = $pep_start + $pep_count - 1;
- $lastphase = $exon->end_phase();
-
- $exon_align->{ 'cdna_start' } = $cdna_start;
- $exon_align->{ 'cdna_end' } = $cdna_end;
- $exon_align->{ 'pep_start' } = $pep_start;
- $exon_align->{ 'pep_end' } = $pep_end;
- $exon_align->{ 'exon' } = $exon;
-
- if( $lastphase == 0 ) {
- $pep_start = $pep_end + 1;
- } else {
- $pep_start = $pep_end;
- }
- $cdna_start = $cdna_end+1;
+ Arg [1] : none
+ Example : $relative_coding_start = $transcript->cdna_coding_start;
+ Description: Retrieves the position of the coding end of this transcript
+ in cdna coordinates (relative to the start of the 5prime end of
+ the transcript, excluding introns, including utrs). This is
+ always te length of the cdna for prediction transcripts because
+ they have no UTRs.
+ Returntype : int
+ Exceptions : none
+ Caller : five_prime_utr, get_all_snps, general
- }
+=cut
- if( ! defined $cdna ) { $cdna = '' };
- return $cdna
+sub cdna_coding_end {
+ my $self = shift;
+ return length($self->spliced_seq);
}
+=head2 transform
-=head1 pep2genomic
-
- Arg 1 : integer start - relative to peptide
- Arg 2 : integer end - relative to peptide
-
- Function : Provides a list of Bio::EnsEMBL::SeqFeatures which
- is the genomic coordinates of this start/end on the peptide
-
- Returns : list of Bio::EnsEMBL::SeqFeature
+ Arg 1 : String $coordinate_system_name
+ Arg [2] : String $coordinate_system_version
+ Example : $ptrans = $ptrans->transform('chromosome', 'NCBI33');
+ $ptrans = $ptrans->transform('clone');
+ Description: Moves this PredictionTranscript to the given coordinate system.
+ If this Transcript has Exons attached, they move as well.
+ A new Transcript is returned or undefined if this PT is not
+ defined in the new coordinate system.
+ Returntype : Bio::EnsEMBL::PredictionTranscript
+ Exceptions : wrong parameters
+ Caller : general
=cut
-sub pep2genomic {
- my ($self,$start,$end) = @_;
+sub transform {
+ my $self = shift;
- if( !defined $end ) {
- $self->throw("Must call with start/end");
+ # catch for old style transform calls
+ if( ref $_[0] && $_[0]->isa( "Bio::EnsEMBL::Slice" )) {
+ throw("transform needs coordinate systems details now," .
+ "please use transfer");
}
- # move start end into translate cDNA coordinates now.
- # much easier!
- $start = 3* $start-2;
- $end = 3* $end;
-
- #
- # Adjust the phase
- #
- my $exons = $self->get_all_Exons;
- if($exons && (my $e = $exons->[0])) {
- $start -= $e->phase;
- $end -= $e->phase;
+ my $new_transcript = Bio::EnsEMBL::Feature::transform($self, @_ );
+ return undef unless $new_transcript;
+
+ #go through the _trans_exon_array so as not to prompt lazy-loading
+ if(exists($self->{'_trans_exon_array'})) {
+ my @new_exons;
+ foreach my $old_exon ( @{$self->{'_trans_exon_array'}} ) {
+ my $new_exon = $old_exon->transform(@_);
+ push(@new_exons, $new_exon);
+ }
+ $new_transcript->{'_trans_exon_array'} = \@new_exons;
}
- return $self->cdna2genomic( $start, $end );
+ return $new_transcript;
}
-=head2 cdna2genomic
+=head2 transfer
- Arg [1] : $start
- The start position in genomic coordinates
- Arg [2] : $end
- The end position in genomic coordinates
- Arg [3] : (optional) $strand
- The strand of the genomic coordinates
- Example : @coords = $transcript->cdna2genomic($start, $end);
- Description: Converts cdna coordinates to genomic coordinates. The
- return value is a list of coordinates and gaps.
- Returntype : list of Bio::EnsEMBL::Mapper::Coordinate and
- Bio::EnsEMBL::Mapper::Gap objects
+ Arg 1 : Bio::EnsEMBL::Slice $destination_slice
+ Example : $ptrans = $ptrans->transfer($slice);
+ Description: Moves this PredictionTranscript to the given slice.
+ If this Transcripts has Exons attached, they move as well.
+ If this transcript cannot be moved then undef is returned
+ instead.
+ Returntype : Bio::EnsEMBL::PredictionTranscript
Exceptions : none
Caller : general
=cut
-sub cdna2genomic {
+sub transfer {
my $self = shift;
- my $start = shift || 1;
- my $end = shift || $self->length;
- if( !defined $end ) {
- $self->throw("Must call with start/end");
- }
+ my $new_transcript = $self->SUPER::transfer( @_ );
+ return undef unless $new_transcript;
- my $mapper = $self->_get_cdna_coord_mapper();
+ if( exists $self->{'_trans_exon_array'} ) {
+ my @new_exons;
+ for my $old_exon ( @{$self->{'_trans_exon_array'}} ) {
+ my $new_exon = $old_exon->transfer( @_ );
+ push( @new_exons, $new_exon );
+ }
- return $mapper->map_coordinates( $self, $start, $end, 1, "cdna" );
-}
+ $new_transcript->{'_trans_exon_array'} = \@new_exons;
+ }
+ return $new_transcript;
+}
+=head2 get_all_Exons
-=head2 genomic2cdna
-
- Arg [1] : $start
- The start position in genomic coordinates
- Arg [2] : $end
- The end position in genomic coordinates
- Arg [3] : (optional) $strand
- The strand of the genomic coordinates (default value 1)
- Arg [4] : (optional) $contig
- The contig the coordinates are on. This can be a slice
- or RawContig, but must be the same object in memory as
- the contig(s) of this transcripts exon(s), because of the
- use of object identity. If no contig argument is specified the
- contig of the first exon is used, which is fine for slice
- coordinates but may cause incorrect mappings in raw contig
- coords if this transcript spans multiple contigs.
- Example : @coords = $transcript->genomic2cdna($start, $end, $strnd, $ctg);
- Description: Converts genomic coordinates to cdna coordinates. The
- return value is a list of coordinates and gaps. Gaps
- represent intronic or upstream/downstream regions which do
- not comprise this transcripts cdna. Coordinate objects
- represent genomic regions which map to exons (utrs included).
- Returntype : list of Bio::EnsEMBL::Mapper::Coordinate and
- Bio::EnsEMBL::Mapper::Gap objects
+ Arg [1] : none
+ Example : my @exons = @{$transcript->get_all_Exons()};
+ Description: Returns an listref of the exons in this transcipr in order.
+ i.e. the first exon in the listref is the 5prime most exon in
+ the transcript.
+ Returntype : a list reference to Bio::EnsEMBL::Exon objects
Exceptions : none
Caller : general
=cut
-sub genomic2cdna {
- my ($self, $start, $end, $strand, $contig) = @_;
-
- unless(defined $start && defined $end) {
- $self->throw("start and end arguments are required\n");
- }
- $strand = 1 unless(defined $strand);
-
- #"ids" in mapper are contigs of exons, so use the same contig that should
- #be attached to all of the exons...
- unless(defined $contig) {
- my @exons = @{$self->get_all_translateable_Exons};
- return () unless(@exons);
- $contig = $exons[0]->contig;
- }
- my $mapper = $self->_get_cdna_coord_mapper;
-
- return $mapper->map_coordinates($contig, $start, $end, $strand, "genomic");
+sub get_all_Exons {
+ my ($self) = @_;
+ if( ! defined $self->{'_trans_exon_array'} && defined $self->adaptor() ) {
+ $self->{'_trans_exon_array'} = $self->adaptor()->db()->
+ get_PredictionExonAdaptor()->fetch_all_by_PredictionTranscript( $self );
+ }
+ return $self->{'_trans_exon_array'};
}
+=head2 display_id
-=head2 _get_cdna_coord_mapper
-
- Args : none
- Example : none
- Description: creates and caches a mapper from "cdna" coordinate system to
- "genomic" coordinate system. Uses Exons to help with that. Only
- calculates in the translateable part.
- Returntype : Bio::EnsEMBL::Mapper( "cdna", "genomic" );
+ Arg [1] : none
+ Example : print $rf->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For prediction transcripts this is
+ the stable_id if it is available otherwise it is an empty
+ string.
+ Returntype : string
Exceptions : none
- Caller : cdna2genomic, pep2genomic
+ Caller : web drawing code
=cut
-sub _get_cdna_coord_mapper {
- my ( $self ) = @_;
-
- if( defined $self->{'_exon_coord_mapper'} ) {
- return $self->{'_exon_coord_mapper'};
- }
-
- #
- # the mapper is loaded with OBJECTS in place of the IDs !!!!
- # the objects are the contigs in the exons
- #
- my $mapper;
- $mapper = Bio::EnsEMBL::Mapper->new( "cdna", "genomic" );
- my @exons = @{$self->get_all_translateable_Exons() };
- my $start = 1;
- for my $exon ( @exons ) {
- $exon->load_genomic_mapper( $mapper, $self, $start );
- $start += $exon->length;
- }
- $self->{'_exon_coord_mapper'} = $mapper;
- return $mapper;
+sub display_id {
+ my $self = shift;
+ return $self->stable_id() || '';
}
+=head2 get_exon_count
-=head2 type
-
- Arg [1] : (optional) string $type
- Example : none
- Description: Getter setter for the type of this prediction transcript
- Returntype : string
- Exceptions : none
- Caller : none
+ Description: DEPRECATED - use get_all_Exons instead
=cut
-sub type {
- my ($self, $type) = @_;
-
- if(defined $type) {
- $self->{'_type'} = $type;
- }
-
- return $self->{'_type'};
+sub get_exon_count {
+ my $self = shift;
+ deprecate('Use scalar(@{$transcript->get_all_Exon()s}) instead');
+ return scalar( @{$self->get_all_Exons} );
}
-=head2 transform
+=head2 set_exon_count
- Arg [1] : (optional) $slice
- The slice to transform this transcript to. If not provided
- the transcript is transformed to raw contig coords.
- Example : $pt->transform($slice)
- Description: Transforms this prediction transcript to slice or chromosomal
- coordinates
- Returntype : none
- Exceptions : none
- Caller : general
+ Description: DEPRECATED - this method does nothing now
=cut
-sub transform {
- my $self = shift;
+sub set_exon_count {
+ deprecate('This method no longer does anything.');
+}
- my @exons;
- foreach my $exon (@{$self->get_all_Exons()}) {
- push @exons, $exon->transform(@_);
- }
- #flush the exons and all related internal caches
- $self->flush_Exons();
+=head2 get_cdna
- # attach the new list of exons to the transcript
- $self->{'exons'} = \@exons;
-}
+ Description : DEPRECATED - use spliced_seq() or translateable_seq instead
+=cut
+sub get_cdna {
+ my $self = shift;
+ deprecate('use spliced_seq instead');
+ return $self->spliced_seq();
+}
1;
diff --git a/modules/Bio/EnsEMBL/Protein.pm b/modules/Bio/EnsEMBL/Protein.pm
index ce55b21113..1c2b3bc0c3 100644
--- a/modules/Bio/EnsEMBL/Protein.pm
+++ b/modules/Bio/EnsEMBL/Protein.pm
@@ -12,97 +12,46 @@
=head1 NAME
-Protein.pm - DESCRIPTION of Object
+Protein.pm - DEPRECATED - Use Bio::EnsEMBL::Translation instead
=head1 SYNOPSIS
-my $protein = Bio::EnsEMBL::Protein->new ( -seq =>$sequence,
- -accession_number => $id,
- -display_id => $id,
- -primary_id => $id,
- -id => $id,
- -desc => $desc,
- -moltype => $moltype,
- );
-
-
=head1 DESCRIPTION
-This object inherit from Bio::SeqI for the implementation and from PrimarySeq
-A protein object hold the basic PrimarySeq requirements, hold annotation, DBlink, protein_features object.
-It will also soon hold family and SNPs objects. The DB adaptor for this object is called Protein_Adaptor
-Most of the calls should be done from the protein object, the protein object will then deal with calling the rigth DBadaptor (ProteinAdaptor, ProteinFeatureAdaptor, ...)
-
-=head1 CONTACT
-
-Describe contact details here
-
-=head1 APPENDIX
+Do not use this object. Use Bio::EnsEMBL::Translation instead.
-The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-
-# Let the code begin...
-
-
package Bio::EnsEMBL::Protein;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::Root;
-
-use Bio::SeqI;
-use Bio::EnsEMBL::Transcript;
-use Bio::SeqIO;
-use Bio::Tools::SeqStats;
-
-# Object preamble - inheriets from Bio::Root::Object
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate);
+use Bio::EnsEMBL::Translation;
-@ISA = qw( Bio::PrimarySeq Bio::SeqI);
+@ISA = qw( Bio::EnsEMBL::Translation );
-=head2 SeqI interface features
-=cut
-
-
-
-=head2 adaptor
-
- Title : adaptor
- Usage : $self->adaptor($adaptor)
- Function: Getter/Setter for the ProteinAdaptor for this object
- Example : my $db = $prot->adaptor()->db();
- Returns : a ProteinAdaptor object
- Args : (optional) a new ProteinAdaptor
-=cut
-
-sub adaptor {
- my ($self, $adaptor) = @_;
-
- if(defined $adaptor) {
- $self->{_adaptor} = $adaptor;
- }
- return $self->{_adaptor};
+sub new {
+ deprecate("The Protein class is deprecated. Use the Translation and " .
+ "Transcript classes instead.\n");
+ return bless {}, 'Bio::EnsEMBL::Protein';
}
-
=head2 species
- Title : species
- Usage : $obj->species($newval)
- Function:
- Returns : value of species
- Args : newvalue (optional)
-
+ Description : DEPRECATED no replacement exists.
=cut
sub species{
my $obj = shift;
+
+ deprecate("You should not have to use this method.");
if( @_ ) {
my $value = shift;
$obj->{'species'} = $value;
@@ -113,12 +62,7 @@ sub species{
=head2 primary_seq
- Title : primary_seq
- Usage : $obj->primary_seq($newval)
- Function:
- Returns : value of primary_seq
- Args : newvalue (optional)
-
+ Description: DEPRECATE do not use
=cut
@@ -131,66 +75,69 @@ sub primary_seq{
=head2 gene
- Title : gene
- Usage : my $gene = $prot->gene()
- Function: Getter/Setter for the gene associated with this protein
- Returns : Bio::EnsEMBL::Gene
- Args : newvalue (optional)
+ Description : DEPRECATED
+ use GeneAdaptor::fetch_by_translation_stable_id instead
=cut
sub gene {
- my ($self, $gene) = @_;
+ my $self = shift;
- if(defined $gene) {
- unless($gene->isa('Bio::EnsEMBL::Gene')) {
- $self->throw("$gene is not a valid Bio::EnsEMBL::Gene object");
- }
- $self->{'_gene'} = $gene;
+ deprecate("Use GeneAdaptor::fetch_by_translation_stable_id");
+
+ my $adaptor = $self->adaptor();
+ if(!$adaptor) {
+ warning("Cannot get gene without attached adaptor");
+ return undef;
}
- return $self->{'_gene'};
+ my $stable_id = $self->stable_id();
+ if(!$stable_id) {
+ warning("Cannot get gene without translation stable_id");
+ return undef;
+ }
+ my $g_adaptor = $adaptor->db->get_GeneAdaptor();
+ return $g_adaptor->fetch_by_translation_stable_id($stable_id);
}
=head2 transcript
- Title : transcript
- Usage : my $transcript = $prot->transcript()
- Function: Getter/Setter for the gene associated with this protein
- Returns : Bio::EnsEMBL::Transcript
- Args : newvalue (optional)
+ Description : DEPRECATED
+ use Bio::EnsEMBL::TranscriptAdaptor::fetch_by_translation_stable_id instead
=cut
sub transcript {
- my ($self, $transcript) = @_;
+ my $self = shift;
+ deprecate("Use TranscriptAdaptor::fetch_by_translation_stable_id");
- if(defined $transcript) {
- unless($transcript->isa('Bio::EnsEMBL::Transcript')) {
- $self->throw("$transcript is not a valid" .
- "Bio::EnsEMBL::Transcript object");
- }
- $self->{'_transcript'} = $transcript;
+ my $adaptor = $self->adaptor();
+ if(!$adaptor) {
+ warning("Cannot get transcript without attached adaptor");
+ return undef;
+ }
+
+ my $stable_id = $self->stable_id();
+ if(!$stable_id) {
+ warning("Cannot get transcript without translation stable_id");
+ return undef;
}
- return $self->{'_transcript'};
+ my $tr_adaptor = $adaptor->db->get_TranscriptAdaptor();
+ return $tr_adaptor->fetch_by_translation_stable_id($stable_id);
}
=head2 molecule
- Title : molecule
- Usage : $obj->molecule($newval)
- Function:
- Returns : value of molecule
- Args : newvalue (optional)
-
+ Description: DEPRECATED - No replacement.
=cut
sub molecule{
my $obj = shift;
+ deprecate("You should not have to use this method.");
if( @_ ) {
my $value = shift;
$obj->{'molecule'} = $value;
@@ -200,18 +147,14 @@ sub molecule{
}
=head2 moltype
-
- Title : moltype
- Usage : $obj->moltype($newval)
- Function:
- Returns : value of moltype
- Args : newvalue (optional)
-
+
+ Description: DEPRECATED - No replacement.
=cut
sub moltype{
my $obj = shift;
+ deprecate("You should not have to use this method.");
if( @_ ) {
my $value = shift;
$obj->{'moltype'} = $value;
@@ -222,57 +165,21 @@ sub moltype{
=head2 top_SeqFeatures
- Title : top_SeqFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED - use TranslationAdaptor::get_all_ProteinFeatures
=cut
sub top_SeqFeatures {
- my ($self,@args) = @_;
-
- return @{$self->get_all_ProteinFeatures};
-}
-
-
-
-
-sub get_all_ProteinFeatures {
my $self = shift;
-
- my @f = ();
-
- push(@f, @{$self->get_all_DomainFeatures()});
-
- push(@f, @{$self->get_all_blastpFeatures()});
-
- push(@f, @{$self->get_all_SigpFeatures()});
-
- push(@f, @{$self->get_all_TransmembraneFeatures()});
-
- push(@f, @{$self->get_all_CoilsFeatures()});
-
- push(@f, @{$self->get_all_LowcomplFeatures()});
-
- return \@f;
+ deprecate("Use TranslationAdaptor::get_all_ProteinFeatures");
+ return @{$self->get_all_ProteinFeatures()};
}
+=head2 get_all_Families
-=head2 get_Family
-
- Title : get_Family
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -293,16 +200,9 @@ sub get_all_Families{
}
-
=head2 add_Family
- Title : add_Family
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -311,83 +211,29 @@ sub add_Family{
unless(defined $value &&
$value->isa('Bio::EnsEMBL::ExternalData::Family::Family')) {
- $self->throw("[$value] is not a Family object");
+ throw("[$value] is not a Family object");
}
push(@{$self->{'_family'}},$value);
}
-=head2 Protein Specific Features
-=cut
-
-=head2 get_all_DomainFeatures
-
- Title : get_all_DomainFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
-
-=cut
-
-sub get_all_DomainFeatures{
- my ($self) = @_;
-
- if (defined ($self->{'_domains'})) {
- return $self->{'_domains'};
- }
-
- my @f = ();
- push(@f,@{$self->get_all_PrintsFeatures()});
- push(@f,@{$self->get_all_PfamFeatures()});
- push(@f,@{$self->get_all_PrositeFeatures()});
- push(@f,@{$self->get_all_SuperfamilyFeatures()});
- push(@f,@{$self->get_all_ProfileFeatures()});
-
- return \@f;
-}
=head2 get_all_ProfileFeatures
- Title : get_all_ProfileFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description : DEPRECATED - use Translation::get_all_ProteinFeatures('pfscan')
=cut
sub get_all_ProfileFeatures{
my ($self) = @_;
-
- unless(defined ($self->{'_profile'})) {
- $self->{'_profile'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor->db()->get_ProteinFeatureAdaptor;
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('PROFILE',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Profile($in);
- }
- }
-
- return ( $self->{'_profile'} || [] );
+ deprecate("Use Translation::get_all_ProteinFeatures('pfscan') instead");
+ return $self->get_all_ProteinFeatures('pfscan');
}
=head2 add_Profile
- Title : add_Profile
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description : DEPRECATED
=cut
@@ -395,7 +241,7 @@ sub add_Profile{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("The Protein Feature added is not defined or is not a "
+ throw("The Protein Feature added is not defined or is not a "
."protein feature object");
}
@@ -405,42 +251,19 @@ sub add_Profile{
=head2 get_all_blastpFeatures
- Title : get_all_blastpFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description : DEPRECATED use Translation::get_all_ProteinFeatures('blastp')
=cut
sub get_all_blastpFeatures{
- my ($self) = @_;
-
- unless(defined ($self->{'_blastp'})) {
- $self->{'_blastp'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('blastp',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_blastp($in);
- }
- }
-
- return ( $self->{'_blastp'} || [] );
+ my $self = shift;
+ deprecate("Use Translation::get_all_ProteinFeatures('blastp') instead");
+ return $self->get_all_ProteinFeatures('blastp');
}
=head2 add_blastp
- Title : add_blastp
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -448,7 +271,7 @@ sub add_blastp{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not defined or is not a protein feature object");
+ throw("[$value] is not defined or is not a protein feature object");
}
push(@{$self->{'_blastp'}},$value);
@@ -456,90 +279,48 @@ sub add_blastp{
=head2 get_all_PrintsFeatures
- Title : get_all_PrintsFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
+ Description: DEPRECATED - use Translation::get_all_ProteinFeatures('prints');
=cut
sub get_all_PrintsFeatures{
my ($self) = @_;
-
- unless(defined ($self->{'_prints'})) {
- $self->{'_prints'} = []; # init in case there are no PRINTS
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('PRINTS',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Prints($in);
- }
- }
- return ( $self->{'_prints'} || [] );
+ deprecate('Use Translation::get_all_ProteinFeatures("prints") instead.');
+ return $self->get_all_ProteinFeatures('prints');
}
=head2 add_Prints
- Title : add_Prints
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
sub add_Prints{
- my ($self,$value) = @_;
-
- if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not a protein feature object");
- }
-
- push(@{$self->{'_prints'}},$value);
+ my ($self,$value) = @_;
+ if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
+ throw("[$value] is not a protein feature object");
+ }
+
+ push(@{$self->{'_prints'}},$value);
}
=head2 get_all_PfamFeatures
- Title : get_all_PfamFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED use Translation::get_all_ProteinFeatures('pfam');
=cut
sub get_all_PfamFeatures{
my ($self) = @_;
-
- unless($self->{'_pfam'}) {
- $self->{'_pfam'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('Pfam',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Pfam($in);
- }
- }
- return ( $self->{'_pfam'} || [] );
+ deprecate('Use Translation::get_all_ProteinFeatures("pfam") instead');
+ return $self->get_all_ProteinFeatures('pfam');
}
+
=head2 add_Pfam
- Title : add_Pfam
- Usage :
- Function:
- Example :
- Returns :
- Args :
+ Description: DEPRECATED
=cut
@@ -547,7 +328,7 @@ sub add_Pfam{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not a protein feature object");
+ throw("[$value] is not a protein feature object");
}
push(@{$self->{'_pfam'}},$value);
@@ -556,39 +337,19 @@ sub add_Pfam{
=head2 get_all_PrositeFeatures
- Title : get_all_PrositeFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
+ Description: DEPRECATED use Translation::get_all_PrositeFeatures('scanprosite');
=cut
sub get_all_PrositeFeatures{
my ($self) = @_;
-
- unless(defined ($self->{'_prosite'})) {
- $self->{'_prosite'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('PROSITE',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Prosite($in);
- }
- }
- return ( $self->{'_prosite'} || [] );
+ deprecate('Use Translation::get_all_ProteinFeatures("scanprosite")');
+ return $self->get_all_ProteinFeatures("scanprosite");
}
=head2 add_Prosite
- Title : add_Prosite
- Usage :
- Function:
- Example :
- Returns :
- Args :
+ Description: DEPRECATED
=cut
@@ -596,7 +357,7 @@ sub add_Prosite{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not a protein feature object");
+ throw("[$value] is not a protein feature object");
}
push(@{$self->{'_prosite'}},$value);
@@ -604,41 +365,20 @@ sub add_Prosite{
=head2 get_all_SigpFeatures
- Title : get_all_SigpFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED - use Translation::get_all_ProteinFeatures instead
=cut
sub get_all_SigpFeatures{
my ($self) = @_;
+ deprecate("Use Translation::get_all_ProteinFeatures('signalp') instead.");
+ return $self->get_all_ProteinFeatures('signalp');
- unless(defined ($self->{'_sigp'})) {
- $self->{'_sigp'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('Signalp',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Sigp($in);
- }
- }
- return ( $self->{'_sigp'} || [] );
}
=head2 add_Sigp
- Title : add_Sigp
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -646,7 +386,7 @@ sub add_Sigp{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not defined or is not a protein feature object");
+ throw("[$value] is not defined or is not a protein feature object");
}
push(@{$self->{'_sigp'}},$value);
}
@@ -654,43 +394,19 @@ sub add_Sigp{
=head2 get_all_TransmembraneFeatures
- Title : get_all_TransmembraneFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED - use Translation::get_all_ProteinFeatures
=cut
sub get_all_TransmembraneFeatures{
- my ($self) = @_;
-
- unless(defined ($self->{'_transmembrane'})) {
- $self->{'_transmembrane'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('Tmhmm',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Transmembrane($in);
- }
- }
-
- return ( $self->{'_transmembrane'} || [] );
-
+ my $self = shift;
+ deprecate("Use Translation::get_all_ProteinFeatures('tmhmm') instead");
+ return $self->get_all_ProteinFeatures('tmhmm');
}
=head2 add_Transmembrane
- Title : add_Transmembrane
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -698,7 +414,7 @@ sub add_Transmembrane{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("The Protein Feature added is not defined" .
+ throw("The Protein Feature added is not defined" .
"or is not a protein feature object");
}
push(@{$self->{'_transmembrane'}},$value);
@@ -706,41 +422,19 @@ sub add_Transmembrane{
=head2 get_all_CoilsFeatures
- Title : get_all_CoilsFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED use Translation::get_all_ProteinFeatures instead
=cut
sub get_all_CoilsFeatures{
- my ($self) = @_;
-
- unless(defined ($self->{'_coils'})) {
- $self->{'_coils'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('ncoils',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Coils($in);
- }
- }
- return ( $self->{'_coils'} || [] );
+ my $self = shift;
+ deprecate("Use Translation::get_all_ProteinFeatures('ncoils') instead.");
+ return $self->get_all_ProteinFeatures('ncoils');
}
=head2 add_Coils
- Title : add_Coils
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -748,46 +442,27 @@ sub add_Coils{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not a protein feature object");
+ throw("[$value] is not a protein feature object");
}
push(@{$self->{'_coils'}},$value);
}
+
=head2 get_all_LowcomplFeatures
- Title : get_all_LowcomplFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
+ Description: DEPRECATED use Translation::get_all_ProteinFeatures('seg');
=cut
sub get_all_LowcomplFeatures{
- my ($self) = @_;
-
- unless (defined ($self->{'_lowcompl'})) {
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db()->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('Seg',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Lowcompl($in);
- }
- }
- return ( $self->{'_lowcompl'} || [] );
+ my $self = shift;
+ deprecate("Use Translation::get_all_ProteinFeatures('seg')");
+ return $self->get_all_ProteinFeatures('seg');
}
=head2 add_LowCompl
- Title : add_LowCompl
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -795,7 +470,7 @@ sub add_Lowcompl{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not a protein feature object");
+ throw("[$value] is not a protein feature object");
}
push(@{$self->{'_lowcompl'}},$value);
@@ -803,41 +478,19 @@ sub add_Lowcompl{
=head2 get_all_SuperfamilyFeatures
- Title : get_all_SuperfamilyFeatures
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+Description: DEPRECATED - use Translation::get_all_ProteinFeatures instead
=cut
sub get_all_SuperfamilyFeatures{
- my ($self) = @_;
-
- unless(defined ($self->{'_superfamily'})) {
- $self->{'_superfamily'} = [];
- my $proteinid = $self->id();
- my $pfa = $self->adaptor()->db->get_ProteinFeatureAdaptor();
- my $array_features =
- $pfa->fetch_all_by_feature_and_dbID('superfamily',$proteinid);
- foreach my $in (@$array_features) {
- $self->add_Superfamily($in);
- }
- }
- return ( $self->{'_superfamily'} || [] );
+ my $self = shift;
+ deprecate("Use Translation::get_all_ProteinFeatures('superfamily') instead");
+ return $self->get_all_ProteinFeatures('superfamily');
}
=head2 add_Superfamily
- Title : add_Superfamily
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
+ Description: DEPRECATED
=cut
@@ -845,94 +498,47 @@ sub add_Superfamily{
my ($self,$value) = @_;
if ((!defined $value) || (!$value->isa('Bio::EnsEMBL::ProteinFeature'))) {
- $self->throw("[$value] is not a protein feature object");
+ throw("[$value] is not a protein feature object");
}
push(@{$self->{'_superfamily'}},$value);
}
-
-
=head2 length
- Title : length
- Usage :my $lenght = $protein->length
- Function:
- Example :
- Returns :length of the aa sequence
- Args :none
-
+ DEPRECATED : use $transcript->translate->length instead
=cut
sub length{
- my ($self) = @_;
- my $length = length($self->seq);
-
- return $length;
-
+ my $self = shift;
+ deprecate("use transcript->translate->length instead");
+ return $self->transcript->translate->length();
}
-=head2 get_all_DBlinks
-
- Title : get_all_DBlinks
- Usage :
- Function:
- Example :
- Returns :
- Args :
-
-
-=cut
-
-sub get_all_DBLinks{
- my ($self) = @_;
-
- unless(defined $self->{'_dblinks'}) {
- $self->{'_dblinks'} = [];
- my $transcript = $self->transcript();
- unless(defined $transcript) {
- throw("Cannot fetch Protein DBLinks without a valid Transcript.\n");
- }
-
- my $dbea = $self->adaptor()->db()->get_DBEntryAdaptor();
- $self->{'_dblinks'} =
- $dbea->fetch_all_by_Translation($transcript->translation);
- }
-
- return $self->{'_dblinks'};
+sub primary_id {
+ my $self = shift;
+ deprecate("Use Translation::dbID or Translation::stable_id instead.");
+ return $self->stable_id || $self->dbID;
}
-=head2 get_all_DASFeatures
+sub id {
+ my $self = shift;
+ deprecate("Use Translation::dbID or Translation::stable_id instead.");
+ return $self->stable_id || $self->dbID;
+}
- Arg [1] : none
- Example : $features = $prot->get_all_DASFeatures;
- Description: Retreives a hash reference to a hash of DAS feature
- sets, keyed by the DNS, NOTE the values of this hash
- are an anonymous array containing:
- (1) a pointer to an array of features;
- (2) a pointer to the DAS stylesheet
- Returntype : hashref of Bio::SeqFeatures
- Exceptions : ?
- Caller : webcode
-=cut
-
-sub get_all_DASFeatures{
- my ($self,@args) = @_;
- my %das_features;
- foreach my $dasfact( $self->adaptor()->db()->_each_DASFeatureFactory ){
- my @featref = $dasfact->fetch_all_by_DBLink_Container( $self );
- $das_features{$dasfact->_dsn} = [@featref];
- }
- return \%das_features;
+sub seq {
+ my $self = shift;
+ deprecate("Use Translation::translate instead.");
+ return $self->transcript->translate()->seq();
}
-
1;
diff --git a/modules/Bio/EnsEMBL/ProteinFeature.pm b/modules/Bio/EnsEMBL/ProteinFeature.pm
index 19a1278b6e..63cfe07c8d 100755
--- a/modules/Bio/EnsEMBL/ProteinFeature.pm
+++ b/modules/Bio/EnsEMBL/ProteinFeature.pm
@@ -16,29 +16,79 @@ ProteinFeature.pm - DESCRIPTION of Object
=head1 SYNOPSIS
-my $feature = new Bio::EnsEMBL::ProteinFeature(-feature1 => $feat1,
- -feature2 => $feat2,);
-=head1 DESCRIPTION
+ my $feature = Bio::EnsEMBL::ProteinFeature->new
+ (-start => $start,
+ -end => $end,
+ -hstart => $hit_start,
+ -hend => $hit_end,
+ -hname => $hit_name);
-This object inherits from Bio::EnsEMBL::FeaturePair. This extension has been implemented to work with the Protein object. Each Protein Feature should be stored in a Protein_FeaturePair object.
+=head1 DESCRIPTION
-This object was formerly named Protein_FeaturePair.
+ProteinFeature objects represent domains or other features of interest
+on a peptide sequence.
=head1 CONTACT
-mongin@ebi.ac.uk
+ Post questions to the EnsEMBL development list ensembl-dev@sanger.ac.uk
=cut
package Bio::EnsEMBL::ProteinFeature;
-use vars qw(@ISA);
+
use strict;
+
use Bio::EnsEMBL::FeaturePair;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use vars qw(@ISA);
@ISA = qw(Bio::EnsEMBL::FeaturePair);
-=head2 idesc
+
+
+=head2 new
+
+ Arg [IDESC] : (optional) string An interpro description
+ Arg [INTERPRO_AC] : (optional) string An interpro accession
+ Arg [...] : named arguments to FeaturePair superclass
+ Example : $pf = Bio::EnsEMBL::ProteinFeature->new(-IDESC => $idesc,
+ -INTERPRO_AC => $iac,
+ @fp_args);
+ Description: Instantiates a Bio::EnsEMBL::ProteinFeature
+ Returntype : Bio::EnsEMBL::FeaturePair
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub new {
+ my $caller = shift;
+
+ my $class = ref($caller) || $caller;
+
+ my ($idesc, $interpro_ac) = rearrange(['IDESC', 'INTERPRO_AC'], @_);
+
+ my $self = $class->SUPER::new(@_);
+
+ #the strand of protein features is always 0
+ $self->{'strand'} = 0;
+ $self->{'idesc'} = $idesc;
+ $self->{'interpro_ac'} = $interpro_ac;
+
+ return $self;
+}
+
+
+
+#do not allow the strand to be set
+sub strand {
+ my $self = shift;
+ return $self->{'strand'};
+}
+
+
+=Head2 idesc
Title : idesc
Usage : $obj->idesc($newval)
@@ -50,15 +100,12 @@ use Bio::EnsEMBL::FeaturePair;
=cut
sub idesc{
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'idesc'} = $value;
- }
- return $obj->{'idesc'};
-
+ my $self = shift;
+ $self->{'idesc'} = shift if(@_);
+ return $self->{'idesc'};
}
+
=head2 interpro_ac
Title : interpro_ac
@@ -71,13 +118,9 @@ sub idesc{
=cut
sub interpro_ac{
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'interpro_ac'} = $value;
- }
- return $obj->{'interpro_ac'};
-
+ my $self = shift;
+ $self->{'interpro_ac'} = shift if(@_);
+ return $self->{'interpro_ac'};
}
diff --git a/modules/Bio/EnsEMBL/RawContig.pm b/modules/Bio/EnsEMBL/RawContig.pm
index b788748747..3fe96788cd 100644
--- a/modules/Bio/EnsEMBL/RawContig.pm
+++ b/modules/Bio/EnsEMBL/RawContig.pm
@@ -6,21 +6,10 @@
# Date : 04.12.2001
#
-
=head1 NAME
-Bio::EnsEMBL::RawContig
- Contig object which represents part of an EMBL Clone.Mostly for
- database usage
-
-=head1 SYNOPSIS
-
-=head1 CONTACT
-
- Arne Stabenau: stabenau@ebi.ac.uk
- Ewan Birney : birney@ebi.ac.uk
-
-=head1 APPENDIX
+Bio::EnsEMBL::RawContig This class is DEPRECATED, use Bio::EnsEMBL::Slice
+instead
=cut
@@ -30,744 +19,117 @@ package Bio::EnsEMBL::RawContig;
use vars qw( @ISA );
use strict;
+use Bio::EnsEMBL::Slice;
+use Bio::EnsEMBL::Clone;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning deprecate);
-use Bio::EnsEMBL::DBSQL::DBAdaptor;
-use Bio::PrimarySeq;
-
-@ISA = qw( Bio::EnsEMBL::Root Bio::PrimarySeqI );
+@ISA = qw( Bio::EnsEMBL::Slice );
=head2 new
- Arg 1 : int dbID
- Arg 2 : Bio::EnsEMBL::DBSQL::RawContigAdaptor adaptor
- Arg 3 : txt contigName
- Arg 4 : Bio::SeqI sequenceContainer
- Arg 5 : int sequenceLength
- Arg 6 : Bio::EnsEMBL::Clone clone
- Arg 7 : int emblCloneBaseOffset
- Arg 7 : int emblCloneContigNumber
- Arg 8 : int emblCloneBaseOffset
- Function : creates RawContig. Neds either dbID and Adaptor or clone and sequence.
- With dbID its connected to DB, with the other it may be stored.
- Returntype: Bio::EnsEMBL::RawContig
- Exceptions: none
- Caller : RawContigAdaptor, Data upload script
+Description: DEPRECATED use Bio::EnsEMBL::Slice instead
=cut
sub new {
my ( $class, @args ) = @_;
- my $self = {};
- bless $self, $class;
-
- my ( $dbID, $adaptor, $name, $sequence, $length,
- $clone, $offset ) = @args;
-
+ deprecate("Bio::EnsEMBL::RawContig is a deprecated class.\n" .
+ "Use Bio::EnsEMBL::Slice instead");
- (defined $dbID) && $self->dbID( $dbID );
- (defined $adaptor) && $self->adaptor( $adaptor );
- (defined $clone) && $self->clone( $clone );
- (defined $sequence) && $self->sequence( $sequence );
- (defined $name) && $self->name( $name );
- (defined $length) && $self->length( $length );
- (defined $offset) && $self->embl_offset( $offset );
-
- return $self;
-}
-
-
-
-sub adaptor {
- my $self = shift;
- my $arg = shift;
-
- ( defined $arg ) &&
- ( $self->{_adaptor} = $arg );
-
- return $self->{_adaptor};
-}
-
-
-
-sub dbID {
- my $self = shift;
- my $arg = shift;
-
- ( defined $arg ) &&
- ( $self->{_dbID} = $arg );
-
- return $self->{_dbID};
-}
-
-
-
-sub name {
- my $self = shift;
- my $arg = shift;
-
- if( defined $arg ) {
- $self->{_name} = $arg ;
- } else {
- if( ! defined $self->{_name} &&
- defined $self->adaptor() ) {
- $self->adaptor->fetch_attributes( $self );
- }
- }
-
- return $self->{_name};
+ return $class->SUPER::new(@args);
}
-# scp: RawContig->primary_seq is used by the pipeline/bioperl
-# (which expects an object to be returned that implements 'id'.
-# As RawContig->primary_seq now returns a RawContig, this
-# object needs to implement 'id' also.
-sub id {
- my ($self) = shift;
- return $self->name || $self->dbID;
-}
-
-
-
-sub length {
+sub embl_offset {
my $self = shift;
- my $arg = shift;
-
- if( defined $arg ) {
- $self->{_length} = $arg ;
- } else {
- if( ! defined $self->{_length} &&
- defined $self->adaptor() ) {
- $self->adaptor->fetch_attributes( $self );
- }
- }
-
- return $self->{_length};
-}
-
-
-
-=head2 seq
-
- Arg [1] : none
- Example : $dna = $contig->seq();
- Description: Obtains the dna sequence of this entire contig as a string
- (positive strand).
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
-
-sub seq {
- my ($self, $arg) = @_;
-
- # Sequence can be set manually
- if($arg) {
- $self->{_seq} = $arg;
- }
- if($self->{_seq}) {
- return $self->{_seq};
- }
-
- #or retrieved from the database
- if($self->adaptor()) {
- my $sa = $self->adaptor->db->dnadb->get_SequenceAdaptor();
- return $sa->fetch_by_RawContig_start_end_strand($self, 1, -1, 1);
- }
-
- $self->warn("RawContig seq not set, and no db is available");
- return '';
-}
-
-
-
-=head2 subseq
-
- Arg [1] : int $start
- The start basepair of the sequence to obtain
- Arg [2] : int $end
- The end basepair of the sequence to obtain
- Arg [3] : (optional) int $strand
- The strand of the sequence to obtain. Default is the contigs
- forward strand.
- Example : $dna = $contig->subseq(1, 1000, -1);
- Description: Obtains a subsequence of this contigs dna sequence
- Returntype : string
- Exceptions : none
- Caller : general
-=cut
-
-sub subseq {
- my ($self, $start, $end, $strand) = @_;
+ deprecate('Use Bio::EnsEMBL::Slice::project instead');
- if ( $end < $start ) {
- $self->throw("End coord is less then start coord to call on RawContig " .
- "subseq.");
- }
+ my @projection = @{$self->project('clone')};
- if ( !defined $strand || ( $strand != -1 && $strand != 1 )) {
- $strand = 1;
+ if(@projection != 1) {
+ warning('This contig not associated with a single clone');
+ return 1;
}
- #if the sequence of this contig has been manually set retrieve its substring
- if(my $str = $self->{_seq}) {
- $str = substr($str, $start -1, $end - $start + 1);
-
- if($strand == -1) {
- $str = reverse $str;
- $str =~ tr/ACTGactg/TGACtgac/;
- }
-
- return $str;
- }
-
- unless($self->adaptor) {
- $self->warn("RawContig::subseq no sequence set and no db available");
- return '';
- }
-
- my $seq_adaptor = $self->adaptor->db->get_SequenceAdaptor();
-
- my $sub_seq = $seq_adaptor->fetch_by_RawContig_start_end_strand($self,
- $start, $end,
- $strand);
- return $sub_seq;
+ #return the start position into the clone
+ return $projection[0]->[2]->start();
}
-=head2 get_base_count
-
- Arg [1] : none
- Example : $gc_content = $contig->get_base_count()->{'%gc'};
- Description: Retrieves a hashref containing the counts of each bases in the
- sequence spanned by this slice. The format of the hash is :
- { 'a' => num,
- 'c' => num,
- 't' => num,
- 'g' => num,
- 'n' => num,
- '%gc' => num }
-
- All bases which are not in the set [A,a,C,c,T,t,G,g] are
- included in the 'n' count. The 'n' count could therefore be
- inclusive of ambiguity codes such as 'y'.
- The %gc is the ratio of GC to AT content as in:
- total(GC)/total(ACTG) * 100
- Returntype : hashref
- Exceptions : none
- Caller : general
-
-=cut
-sub get_base_count {
+sub clone {
my $self = shift;
- my $seq = $self->seq();
- my $len = $self->length();
+ deprecate('Use Bio::EnsEMBL::Slice::project instead');
- my $a = $seq =~ tr/Aa/Aa/;
- my $c = $seq =~ tr/Cc/Cc/;
- my $t = $seq =~ tr/Tt/Tt/;
- my $g = $seq =~ tr/Gg/Gg/;
+ my @projection = @{$self->project('clone')};
- my $gc_content = 0;
- if($a || $g || $c || $t) { #avoid divide by 0
- $gc_content = sprintf( "%1.2f", (($g + $c)/($a + $g + $t + $c)) * 100);
+ if(@projection != 1) {
+ warning('This contig is associated with ' .scalar(@projection).' clones.');
+ return undef;
}
- return {'a' => $a,
- 'c' => $c,
- 't' => $t,
- 'g' => $g,
- 'n' => $len - $a - $c - $t - $g,
- '%gc' => $gc_content};
-}
+ my $clone = $projection[0]->[2];
+ #get full clone instead of potentially partial clone
+ $clone = $self->adaptor->fetch_by_region($clone->coord_system->name(),
+ $clone->seq_region_name(),
+ undef,
+ undef,
+ undef,
+ $clone->coord_system->version());
-=head2 get_repeatmasked_seq
- Arg [1] : string \@logic_names (optional)
- Arg [2] : int $soft_masking_enable (optional)
- Example : $slice->get_repeatmasked_seq or
- $slice->get_repeatmasked_seq(['RepeatMask'],1)
- Description: Returns Bio::PrimarySeq containing the masked
- (repeat replaced by N)
- or soft-masked (when Arg[2]=1, repeat in lower case while
- non repeat in upper case) sequence corresponding to the
- Slice object. Will only work with database connection to get
- repeat features.
- Returntype : Bio::PrimarySeq
- Exceptions : none
- Caller : general.
-
-=cut
-
-sub get_repeatmasked_seq {
- my ($self, $logic_names, $soft_mask) = @_;
-
- unless ($logic_names && @$logic_names) {
- $logic_names = [ '' ];
- }
-
- unless (defined $soft_mask) {
- $soft_mask = 0;
- }
-
- my $repeats = [];
-
- foreach my $l (@$logic_names) {
- push @{$repeats}, @{$self->get_all_RepeatFeatures($l)};
- }
-
- my $dna = $self->seq();
- my $masked_dna = $self->_mask_features($dna,$repeats,$soft_mask);
- my $masked_seq = Bio::PrimarySeq->new('-seq' => $masked_dna,
- '-display_id' => $self->name,
- '-primary_id' => $self->name,
- '-moltype' => 'dna'
- );
- return $masked_seq;
-}
-
-
-=head2 _mask_features
-
- Arg [1] : string $dna_string
- Arg [2] : array_ref \@repeats
- reference to a list Bio::EnsEMBL::RepeatFeature
- give the list of coordinates to replace with N or with lower case
- Arg [3] : int $soft_masking_enable (optional)
- Example :
- Description: replaces string positions described in the RepeatFeatures
- with Ns (default setting), or with the lower case equivalent (soft masking)
- Returntype : string
- Exceptions : none
- Caller : get_repeatmasked_seq
-
-=cut
-
-sub _mask_features {
- my ($self,$dnastr,$repeats,$soft_mask) = @_;
-
- # explicit CORE::length call, to avoid confusion with Slice::length method
- my $dnalen = CORE::length($dnastr);
-
- REP:foreach my $f (@{$repeats}) {
-
- my $start = $f->start;
- my $end = $f->end;
- my $length = ($end - $start) + 1;
-
- # check if we get repeat completely outside of expected slice range
- if ($end < 1 || $start > $dnalen) {
- $self->warn("Repeat completely outside RawContig coordinates!".
- "That should not happen! repeat_start $start or " .
- "repeat_end $end not within [1-$dnalen] RawContig " .
- "range coordinates\n");
- next REP;
- }
-
- # repeat partly outside slice range, so correct
- # the repeat start and length to the slice size if needed
- if ($start < 1) {
- $start = 1;
- $length = ($end - $start) + 1;
- }
-
- # repeat partly outside slice range, so correct
- # the repeat end and length to the slice size if needed
- if ($end > $dnalen) {
- $end = $dnalen;
- $length = ($end - $start) + 1;
- }
-
- $start--;
-
- my $padstr;
-
- if ($soft_mask) {
- $padstr = lc substr ($dnastr,$start,$length);
- } else {
- $padstr = 'N' x $length;
- }
- substr ($dnastr,$start,$length) = $padstr;
- }
- return $dnastr;
+ #rebless the slice as a Bio::EnsEMBL::Clone so old method calls still work
+ return bless $clone, 'Bio::EnsEMBL::Clone';
}
-=head2 get_all_PredictionTranscripts
-
- Args : none
- Function : connect to database through set adaptor and retrieve the
- PredictionFeatures for this contig.
- Returntype: listref Bio::EnsEMBL::PredictionTranscript
- (previously this returned a SeqFeature)
- Exceptions: none
- Caller : general
-
-=cut
-
-sub get_all_PredictionTranscripts {
- my $self = shift;
- my $logic_name = shift;
-
- if( ! defined $self->adaptor() ) {
- $self->warn( "Need db connection for get_all_PredictionFeatures()" );
- return ();
- }
-
- my $pta = $self->adaptor->db->get_PredictionTranscriptAdaptor();
-
- return $pta->fetch_all_by_RawContig($self, $logic_name);
-}
-
-
-
-=head2 get_all_RepeatFeatures
-
- Args : none
- Function : connect to database through set adaptor and retrieve the
- repeatfeatures for this contig.
- Returntype: listref Bio::EnsEMBL::RepeatFeature
- Exceptions: none
- Caller : general, get_repeatmasked_seq()
-
-=cut
-
-sub get_all_RepeatFeatures {
- my $self = shift;
- my $logic_name = shift;
-
- if( ! defined $self->adaptor() ) {
- $self->warn( "Need db connection for get_all_RepeatFeatures()" );
- return ();
- }
-
- my $rfa = $self->adaptor()->db->get_RepeatFeatureAdaptor();
-
- return $rfa->fetch_all_by_RawContig( $self , $logic_name);
-}
-
-
-
-=head2 get_all_SimilarityFeatures
-
- Args : none
- Function : connect to database through set adaptor and retrieve the
- SimilarityFeatures for this contig.
- Returntype: list Bio::EnsEMBL::FeaturePair
- Exceptions: none
- Caller : general
-
-=cut
-
-sub get_all_SimilarityFeatures {
- my ($self, $logic_name, $score) = @_;
-
- if( ! defined $self->adaptor() ) {
- $self->warn( "Need db connection for get_all_SimilarityFeatures()" );
- return ();
- }
-
- my @out;
- my $dafa = $self->adaptor->db->get_DnaAlignFeatureAdaptor();
- my $pafa = $self->adaptor->db->get_ProteinAlignFeatureAdaptor();
- push @out, @{$dafa->fetch_all_by_RawContig_and_score($self, $score, $logic_name)};
- push @out, @{$pafa->fetch_all_by_RawContig_and_score($self, $score, $logic_name)};
-
- return \@out;
-}
-
-
-=head2 get_all_DnaAlignFeatures
-
- Arg [1] : (optional) string $logic_name
- The name of the analysis performed on the dna align features
- to obtain.
- Arg [2] : (optional) float $score
- The mimimum score of the features to retrieve
- Example : @dna_align_feats = $contig->get_all_DnaAlignFeatures()
- Description: Retrieves the DnaDnaAlignFeatures which overlap this contig
- Returntype : list of Bio::EnsEMBL::DnaDnaAlignFeatures
- Exceptions : none
- Caller :general
-
-=cut
-
-sub get_all_DnaAlignFeatures {
- my ($self, $logic_name, $score) = @_;
-
-
- if( ! defined $self->adaptor() ) {
- $self->warn( "Need db connection for get_all_DnaAlignFeatures()" );
- return ();
- }
-
- my $dafa = $self->adaptor->db->get_DnaAlignFeatureAdaptor();
-
- return $dafa->fetch_all_by_RawContig_and_score($self,$score, $logic_name);
-}
-
-
-
-=head2 get_all_ProteinAlignFeatures
-
- Arg [1] : (optional) string $logic_name
- The name of the analysis performed on the protein align features
- to obtain.
- Arg [2] : (optional) float $score
- The mimimum score of the features to retrieve
- Example : @pep_align_feats = $contig->get_all_ProteinAlignFeatures()
- Description: Retrieves the PepDnaAlignFeatures which overlap this contig.
- Returntype : list of Bio::EnsEMBL::PepDnaAlignFeatures
- Exceptions : none
- Caller : general
-
-=cut
-
-sub get_all_ProteinAlignFeatures {
- my ($self, $logic_name, $score) = @_;
-
-
- if( ! defined $self->adaptor() ) {
- $self->warn( "Need db connection for get_all_ProteinAlignFeatures()" );
- return ();
- }
-
- my $pafa = $self->adaptor()->db()->get_ProteinAlignFeatureAdaptor();
-
- return $pafa->fetch_all_by_RawContig_and_score($self, $score, $logic_name);
-}
-
-
-
-=head2 get_all_SimpleFeatures
-
- Arg [1] : (optional) string $logic_name
- The name of the analysis performed on the simple features
- to obtain.
- Arg [2] : (optional) float $score
- The mimimum score of the features to retrieve
- Example : @simple_feats = $contig->get_all_SimpleFeatures()
- Description: Retrieves the SimpleFeatures which overlap this contig.
- Returntype : list of Bio::EnsEMBL::DnaDnaAlignFeature
- Exceptions : none
- Caller : general
-
-=cut
-
-sub get_all_SimpleFeatures {
- my ($self, $logic_name, $score) = @_;
-
-
- if( ! defined $self->adaptor() ) {
- $self->warn( "Need db connection for get_all_SimpleFeatures()" );
- return ();
- }
-
- my $sfa = $self->adaptor()->db()->get_SimpleFeatureAdaptor();
-
- return $sfa->fetch_all_by_RawContig_and_score($self, $score, $logic_name);
-}
-
-
-sub embl_offset {
- my $self = shift;
- my $arg = shift;
-
- if( defined $arg ) {
- $self->{_embl_offset} = $arg ;
- } else {
- if( ! defined $self->{_embl_offset} &&
- defined $self->adaptor() ) {
- $self->adaptor->fetch_attributes( $self );
- }
- }
-
- return $self->{_embl_offset};
-}
-
-
-sub clone {
- my $self = shift;
- my $arg = shift;
-
- if( defined $arg ) {
- $self->{_clone} = $arg ;
- } else {
- if( ! defined $self->{_clone} &&
- defined $self->adaptor() ) {
- if( !defined $self->_clone_id() ) {
- $self->adaptor->fetch_attributes($self);
- }
- $self->{_clone} =
- $self->adaptor->db->get_CloneAdaptor->fetch_by_dbID($self->_clone_id());
- }
- }
-
- return $self->{_clone};
-}
-
-sub _clone_id {
- my $self = shift;
- my $arg = shift;
-
- if( defined $arg ) {
- $self->{_clone_id} = $arg ;
- }
-
- return $self->{_clone_id};
-}
-
-
-
-
-=head2 get_all_ExternalFeatures
-
- Arg [1] : (optional) string $track_name
- If specified only features from ExternalFeatureAdaptors with
- the track name $track_name are retrieved. If not set, all
- features from every ExternalFeatureAdaptor are retrieved.
- Example : @xfeatures = @{$contig->get_all_ExternalFeatures};
- Description: Retrieves features on this contig from external feature adaptors
- Returntype : listref of Bio::SeqFeatureI implementing objects in contig
- coordinates
- Exceptions : none
- Caller : general
-
-=cut
-
-sub get_all_ExternalFeatures {
- my ($self, $track_name) = @_;
-
- my $features = [];
-
- my $xfa_hash = $self->adaptor->db->get_ExternalFeatureAdaptors;
- my @xf_adaptors = ();
-
- if($track_name) {
- #use a specific adaptor
- push @xf_adaptors, $xfa_hash->{$track_name};
- } else {
- #use all of the adaptors
- push @xf_adaptors, values %$xfa_hash;
- }
-
- foreach my $xfa (@xf_adaptors) {
- push @$features, @{$xfa->fetch_all_by_RawContig($self)};
- }
-
- return $features;
-}
=head2: ctg2genomic
- Arg [1] : $start
- The start position in contig coordinates
- Arg [2] : $end
- The end position in contig coordinates
- Arg [3] : (optional) $strand
- The strand of the contig coordinates
- Example : @coords = $transcript->ctg2genomic($start, $end);
- Description: Converts contig coordinates to genomic coordinates. The
- return value is a list of coordinates and gaps.
- Returntype : list of Bio::EnsEMBL::Mapper::Coordinate and
- Bio::EnsEMBL::Mapper::Gap objects
- Exceptions : none
- Caller : general
+ Description: use Bio::EnsEMBL::Slice instead
=cut
-
sub ctg2genomic{
# Map the internal ID onto the golden path
- my $self = shift;
+ my $self = shift;
my $start = shift || 1;
my $end = shift || $self->length;
- my $strand = shift || 1;
+ my $strand = shift || 1;
+
+ deprecate('Use Bio::EnsEMBL::Slice instead');
my $db = $self->adaptor->db();
my $aa = $db->get_AssemblyMapperAdaptor();
- my $ma = $aa->fetch_by_type( $db->assembly_type );
- return $ma->map_coordinates_to_assembly( $self->dbID, $start, $end,
- $strand );
-}
-
-=head2 Methods included only for BioPerl compliance
-=cut
-###############################################################################
-
-=head2 display_id
-
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance.
- Returntype : string
- Exceptions : none
- Caller : none
-
-=cut
-
-sub display_id{
- my $self = shift;
-
- return $self->id();
-}
+ my $csa = $db->get_CoordSystemAdaptor();
-=head2 desc
-
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance
- Returntype : none
- Exceptions : none
- Caller : none
-
-=cut
+ my $top_level = $csa->fetch_top_level();
+ my $ma = $aa->fetch_by_CoordSystems($top_level, $self->coord_system);
-sub desc{
- my $self = shift;
- return "RawContig, no description";
+ return $ma->map_coordinates_to_assembly( $self->seq_region_name,
+ $start,
+ $end,
+ $strand,
+ $self->coord_system);
}
-=head2 moltype
-
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance
- Returntype : none
- Exceptions : none
- Caller : none
-=cut
-sub moltype {
+#
+# The name that is wanted is actually the seq_region name
+#
+sub name {
my $self = shift;
- return 'dna';
-}
-
-=head2 accession_number
-
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance
- Returntype : none
- Exceptions : none
- Caller : none
-=cut
-
-sub accession_number {
- my $self = shift;
- return $self->dbID();
+ return $self->seq_region_name();
}
diff --git a/modules/Bio/EnsEMBL/RepeatConsensus.pm b/modules/Bio/EnsEMBL/RepeatConsensus.pm
index d48a49c25b..48ac13f8e5 100644
--- a/modules/Bio/EnsEMBL/RepeatConsensus.pm
+++ b/modules/Bio/EnsEMBL/RepeatConsensus.pm
@@ -1,96 +1,169 @@
-
-### Bio::EnsEMBL::RepeatConsensus
+use strict;
package Bio::EnsEMBL::RepeatConsensus;
-use strict;
-use Bio::PrimarySeqI;
-use Bio::EnsEMBL::Root;
-use vars '@ISA';
-
-@ISA = qw(Bio::EnsEMBL::Root Bio::PrimarySeqI);
+use Bio::EnsEMBL::Storable;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+use vars qw(@ISA);
+@ISA = qw(Bio::EnsEMBL::Storable);
+
+
+=head2 new
+
+ Arg [NAME] : string (optional)
+ The name of this repeat consensus
+ Arg [LENGTH]: int (optional)
+ The length of the repeat consensus sequence
+ Arg [REPEAT_CLASS]: string (optional)
+ The type of repeat consensus
+ Arg [REPEAT_CONSENSUS]: string (optional)
+ The sequence of this repeat consensus
+ Arg [...]: Named arguments to superclass constructor
+ (see Bio::EnsEMBL::Storable)
+ Example : $rc = Bio::EnsEMBL::RepeatConsensus->new
+ (-REPEAT_CONSENSUS => 'AATG'
+ -NAME => '(AATG)n',
+ -REPEAT_CLASS => 'Simple_repeat',
+ -LENGTH => '4',
+ -DBID => 1023,
+ -ADAPTOR => $rc_adaptor);
+ Description: Creates a new Bio::EnsEMBL::RepeatFeature object
+ Returntype : Bio::EnsEMBL::RepeatFeature
+ Exceptions : none
+ Caller : RepeatFeatureAdaptors
+
+=cut
sub new {
- my( $pkg ) = @_;
-
- return bless {}, $pkg;
-}
+ my $caller = shift;
+
+ my $class = ref($caller) || $caller;
+
+ my $self = $class->SUPER::new(@_);
+
+ my ($name, $length, $repeat_class, $repeat_consensus) =
+ rearrange(['NAME', 'LENGTH', 'REPEAT_CLASS', 'REPEAT_CONSENSUS'], @_);
-sub moltype { return 'dna' };
-sub alphabet { return 'dna' };
+ $self->{'name'} = $name;
+ $self->{'length'} = $length;
+ $self->{'repeat_class'} = $repeat_class;
+ $self->{'repeat_consensus'} = $repeat_consensus;
-sub dbID {
- my( $self, $db_id ) = @_;
-
- if ($db_id) {
- $self->{'_db_id'} = $db_id;
- }
- return $self->{'_db_id'};
+ return $self;
}
-# Alias primary_id method to dbID
-*primary_id = \&dbID;
+
+=head2 name
+
+ Arg [1] : string $name (optional)
+ Example : $name = $repeat_consensus->name()
+ Description: Getter/Setter for the name of this repeat_consensus
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
sub name {
- my( $self, $name ) = @_;
-
- if ($name) {
- $self->{'_name'} = $name;
- }
- return $self->{'_name'};
+ my $self = shift;
+ $self->{'name'} = shift if(@_);
+ return $self->{'name'};
}
-# Alias display_id and accession_number methods to name
-*display_id = \&name;
-*accession_number = \&name;
+
+=head2 length
+
+ Arg [1] : int $length (optional)
+ Example : $length = $repeat_consensus->length()
+ Description: Getter/Setter for the length of this repeat_consensus
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
sub length {
- my( $self, $length ) = @_;
-
- if ($length) {
- $self->{'_length'} = $length;
- }
- return $self->{'_length'};
+ my $self = shift;
+ $self->{'length'} = shift if(@_);
+ return $self->{'length'};
}
+
+=head2 repeat_class
+
+ Arg [1] : string $class (optional)
+ The class of
+ Example : $class = $repeat_consensus->repeat_class()
+ Description: Getter/Setter for the class of this repeat_consensus
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
sub repeat_class {
- my( $self, $repeat_class ) = @_;
-
- if ($repeat_class) {
- $self->{'_repeat_class'} = $repeat_class;
- }
- return $self->{'_repeat_class'};
+ my $self = shift;
+ $self->{'repeat_class'} = shift if(@_);
+ return $self->{'repeat_class'};
}
+
+=head2 desc
+
+ Arg [1] : none
+ Example : $desc = $repeat_consensus->desc()
+ Description: Getter for the description of this repeat consensus as extracted
+ from the repeat_class. This method is probably useless.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
sub desc {
- my( $self ) = @_;
-
- my $class = $self->repeat_class or return;
- return "class=$class";
+ my $self = shift;
+ my $class = $self->repeat_class or return;
+ return "class=$class";
}
-sub adaptor {
- my( $self, $adaptor ) = @_;
-
- if ($adaptor) {
- $self->{'_adaptor'} = $adaptor;
- }
- return $self->{'_adaptor'};
-}
+
+
+=head2 repeat_consensus
+
+ Arg [1] : string $consensus_seq (optional)
+ The sequence of this repeat consensus
+ Example : $consensus = $repeat_consensus->repeat_consensus();
+ Description: Getter/Setter for the sequence of this repeat_consensus.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
sub repeat_consensus {
- my( $self, $repeat_consensus ) = @_;
-
- if ($repeat_consensus) {
- $self->{'_repeat_consensus'} = $repeat_consensus;
- }
- return $self->{'_repeat_consensus'};
+ my $self = shift;
+ $self->{'repeat_consensus'} = shift if(@_);
+ return $self->{'repeat_consensus'};
}
+
+
+=head2 seq
+
+ Arg [1] : none
+ Example : none
+ Description: Returns the repeat consensus. This method is useless - Use
+ repeat_consensus() instead.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
sub seq {
- my( $self ) = @_;
-
- return $self->repeat_consensus;
+ my( $self ) = @_;
+ return $self->repeat_consensus;
}
1;
diff --git a/modules/Bio/EnsEMBL/RepeatFeature.pm b/modules/Bio/EnsEMBL/RepeatFeature.pm
index 12407b1bbe..5d48e7338f 100644
--- a/modules/Bio/EnsEMBL/RepeatFeature.pm
+++ b/modules/Bio/EnsEMBL/RepeatFeature.pm
@@ -1,14 +1,69 @@
+# EnsEMBL module for Bio::EnsEMBL::RepeatFeature
+#
+# Copyright (c) 2003 EnsEMBL
+#
-### Bio::EnsEMBL::RepeatFeature
+=head1 NAME
+
+Bio::EnsEMBL::RepeatFeature - A feature representing a repeat on a piece of
+sequence.
+
+=head1 SYNOPSIS
+
+ my $rf = new Bio::EnsEMBL::Feature(-start => 100,
+ -end => 220,
+ -strand => -1,
+ -slice => $slice
+ -analysis => $analysis
+ -repeat_consensus => $rc,
+ -hstart => 10,
+ -hend => 100,
+ -hstrand => 1,
+ -score => 83.2
+ );
+
+ my $hstart = $feat->hstart;
+ my $hend = $feat->hend;
+
+ #move the feature to the chromosomal coordinate system
+ $feature = $feature->transform('chromosome');
+
+ #move the feature to a different slice (possibly on another coord system)
+ $feature = $feature->transfer($new_slice);
+
+ #project the feature onto another coordinate system possibly across
+ #boundaries:
+ @projection = @{$feature->project('contig')};
+
+ #change the start, end, and strand of the feature in place
+ $feature->move($new_start, $new_end, $new_strand);
+
+=head1 DESCRIPTION
+
+This a feature representing a repeat region on a sequence
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL development list: ensembl-dev@ebi.ac.uk
+
+=head1 APPENDIX
+
+The rest of the documentation details each of the object methods.
+Internal methods are usually preceded with a _
+
+=cut
package Bio::EnsEMBL::RepeatFeature;
use strict;
-use Bio::EnsEMBL::SeqFeature;
+use Bio::EnsEMBL::Feature;
+
+use Bio::EnsEMBL::Utils::Exception qw(throw);
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
use vars '@ISA';
-@ISA = qw{ Bio::EnsEMBL::SeqFeature };
+@ISA = qw{ Bio::EnsEMBL::Feature };
=head2 new_fast
@@ -16,10 +71,10 @@ use vars '@ISA';
Arg [1] : hash reference $hashref
Example : none
Description: This is an ultra fast constructor which requires knowledge of
- the objects internals to be used. It is only used by
+ the objects internals to be used. It is only used by
RepeatFeatureAdaptors (when thousands of repeats need to be
- quickly created). The SeqFeature superclass constructor 'new'
- should be used in most instances.
+ quickly created). The constructor 'new' should be used in
+ most instances.
Returntype : Bio::EnsEMBL::RepeatFeature
Exceptions : none
Caller : RepeatFeatureAdaptors
@@ -28,35 +83,56 @@ use vars '@ISA';
sub new_fast {
my ($class, $hashref) = @_;
-
+
return bless $hashref, $class;
}
-
-=head2 adaptor
-
- Arg [1] : (optional) Bio::EnsEMBL::DBSQL::RepeatFeatureAdaptor $adaptor
- Example : $adaptor = $repeat->adaptor;
- Description: The adaptor which performs database requests for this object.
- This should be set when the object is stored in the database or
- retrieved from the database.
- Returntype : Bio::EnsEMBL::DBSQL::RepeatFeatureAdaptor
+=head2 new
+
+ Arg [REPEAT_CONSENSUS] : Bio::EnsEMBL::RepeatConsensus (optional)
+ The repeat consensus for this repeat feature
+ Arg [HSTART] : int (optional)
+ The hit start on the consensus sequence
+ Arg [HEND] : int (optional)
+ The hit end on the consensus sequence
+ Arg [SCORE] : float (optional)
+ The score
+ Arg [...] : Named arguments to superclass constructor
+ (see Bio::EnsEMBL::Feaure)
+ Example : $rf = Bio::EnsEMBL::RepeatFeature->new(-REPEAT_CONSENSUS => $rc,
+ -HSTART => 10,
+ -HEND => 100,
+ -SCORE => 58.0,
+ -START => 1_000_100,
+ -END => 1_000_190,
+ -STRAND => 1,
+ -ANALYSIS => $an,
+ -SLICE => $chr_slice);
+ Description: Creates a new Bio::EnsEMBL::RepeatFeature object
+ Returntype : Bio::EnsEMBL::RepeatFeature
Exceptions : none
- Caller : general
+ Caller : RepeatFeatureAdaptors
=cut
-sub adaptor {
- my ($self, $adaptor) = @_;
-
- if(defined $adaptor) {
- $self->{'_adaptor'} = $adaptor;
- }
-
- return $self->{'_adaptor'};
-}
+sub new {
+ my $caller = shift;
+
+ my $class = ref($caller) || $caller;
+
+ my $self = $class->SUPER::new(@_);
+ my ($repeat_consensus, $hstart, $hend, $score) =
+ rearrange(['REPEAT_CONSENSUS','HSTART','HEND','SCORE'], @_);
+
+ $self->repeat_consensus($repeat_consensus);
+ $self->{'hstart'} = $hstart;
+ $self->{'hend'} = $hend;
+ $self->{'score'} = $score;
+
+ return $self;
+}
=head2 repeat_consensus
@@ -71,80 +147,57 @@ sub adaptor {
=cut
sub repeat_consensus {
- my( $self, $con ) = @_;
-
- if (defined $con) {
- $self->throw("$con is not a RepeatConsensus")
- unless $con->isa("Bio::EnsEMBL::RepeatConsensus");
- $self->{'_repeat_consensus'} = $con;
+ my $self = shift;
+
+ if(@_) {
+ my $rc = shift;
+ if(defined($rc)) {
+ if(!ref($rc) || !$rc->isa('Bio::EnsEMBL::RepeatConsensus')) {
+ throw('RepeatConsensus arg must be a Bio::EnsEMBL::RepeatConsensus');
+ }
+ }
+ $self->{'repeat_consensus'} = $rc;
}
- return $self->{'_repeat_consensus'};
+
+ return $self->{'repeat_consensus'};
}
-=head2 dbID
+=head2 hstart
- Arg [1] : (optional) $db_id
- Example : $dbID = $repeat->dbID
- Description: getter/setter for this objects internal database identifier
+ Arg [1] : (optional) int $hstart
+ Example : $hit_start = $repeat->hstart;
+ Description: Getter/Setter for the start bp of this repeat match on the
+ consensus sequence.
Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub dbID {
- my( $self, $db_id ) = @_;
-
- if (defined $db_id) {
- $self->{'_db_id'} = $db_id;
- }
- return $self->{'_db_id'};
-}
-
-
-=head2 analysis
-
- Arg [1] : (optional) Bio::EnsEMBL::Analysis
- Example : $analysis = $repeat_feat->analysis;
- Description: Getter/Setter for the analysis that was used to generate this
- object
- Returntype : Bio::EnsEMBL::Analysis
- Exceptions : none
+ Exceptions : none
Caller : general
=cut
-sub analysis {
- my( $self, $analysis ) = @_;
-
- if ($analysis) {
- $self->{'_analysis'} = $analysis;
- }
- return $self->{'_analysis'};
+sub hstart {
+ my $self = shift;
+ $self->{'hstart'} = shift if(@_);
+ return $self->{'hstart'};
}
-=head2 hstart
+=head2 score
- Arg [1] : (optional) int $hstart
- Example : $hit_start = $repeat->hstart;
- Description: Getter/Setter for the start bp of this repeat match on the
- consensus sequence.
+ Arg [1] : (optional) float $score
+ Example : $score = $repeat->score();
+ Description: Getter/Setter for the score of this repeat feature
Returntype : int
- Exceptions : none
+ Exceptions : none
Caller : general
=cut
-sub hstart {
- my( $self, $hstart ) = @_;
-
- if ($hstart) {
- $self->{'_hstart'} = $hstart;
- }
- return $self->{'_hstart'};
+sub score {
+ my $self = shift;
+ $self->{'score'} = shift if(@_);
+ return $self->{'score'};
}
@@ -162,47 +215,56 @@ sub hstart {
=cut
sub hend {
- my( $self, $hend ) = @_;
-
- if ($hend) {
- $self->{'_hend'} = $hend;
- }
- return $self->{'_hend'};
+ my $self = shift;
+ $self->{'hend'} = shift if(@_);
+ return $self->{'hend'};
}
=head2 hstrand
- Arg [1] : none
+ Arg [1] : none
Example : none
Description: always returns 1. method exists for consistancy with other
features.
Returntype : int
Exceptions : none
- Caller :
+ Caller :
=cut
-sub hstrand {
- return 1;
+sub hstrand {
+ return 1;
}
-sub to_FTString {
- my( $self ) = @_;
-
- my $start = $self->start or $self->throw("start not set");
- my $end = $self->end or $self->throw("end not set");
- my $strand = $self->strand;
- $self->throw("strand not set") unless defined($strand);
-
- my $loc = "$start..$end";
- if ($strand == -1) {
- $loc = "complement($loc)";
- }
-}
+=head2 display_id
+ Arg [1] : none
+ Example : print $rf->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For repeat_features this is the
+ name of the repeat consensus if it is available otherwise it is
+ an empty string.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
+
+=cut
+
+sub display_id {
+ my $self = shift;
+
+ my $id = '';
+
+ my $rc = $self->{'repeat_consensus'};
+ if($rc) {
+ $id = $rc->name();
+ }
+
+ return $id;
+}
1;
diff --git a/modules/Bio/EnsEMBL/RepeatMaskedSlice.pm b/modules/Bio/EnsEMBL/RepeatMaskedSlice.pm
new file mode 100644
index 0000000000..0a55b949a3
--- /dev/null
+++ b/modules/Bio/EnsEMBL/RepeatMaskedSlice.pm
@@ -0,0 +1,234 @@
+#
+# Ensembl module for Bio::EnsEMBL::RepeatMaskedSlice
+#
+#
+#
+# You may distribute this module under the same terms as perl itself
+
+# POD documentation - main docs before the code
+
+=head1 NAME
+
+Bio::EnsEMBL::RepeatMaskedSlice - Arbitary Slice of a genome
+
+=head1 SYNOPSIS
+
+ $sa = $db->get_SliceAdaptor;
+
+ $slice = $sa->fetch_by_region('chromosome', 'X', 1_000_000, 2_000_000);
+
+ $repeat_masked_slice = $sa->get_repeatmasked_seq();
+
+ #get repeat masked sequence:
+ my $dna = $repeat_masked_slice->seq();
+ $dna = $repeat_masked_slice->subseq(1, 1000);
+
+
+=head1 DESCRIPTION
+
+This is a specialised B<Bio::EnsEMBL::Slice> class that is used to
+retrieve repeat masked genomic sequence rather than normal genomic
+sequence.
+
+=head1 AUTHOR - Graham McVicker
+
+=head1 CONTACT
+
+This modules is part of the Ensembl project http://www.ensembl.org
+
+Questions can be posted to the ensembl-dev mailing list:
+ensembl-dev@ebi.ac.uk
+
+=head1 METHODS
+
+=cut
+
+
+package Bio::EnsEMBL::RepeatMaskedSlice;
+
+use strict;
+use warnings;
+
+use Bio::EnsEMBL::Slice;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+
+use vars qw(@ISA);
+
+@ISA = ('Bio::EnsEMBL::Slice');
+
+
+
+=head2 new
+
+ Arg [-REPEAT_MASK] : The logic name of the repeats to be used for masking.
+ If not provided, all repeats in the database are used.
+ Arg [...] : Named superclass arguments. See B<Bio::EnsEMBL::Slice>.
+ Example : my $slice = Bio::EnsEMBL::RepeatMaskedSlice->new
+ (-START => $start,
+ -END => $end,
+ -STRAND => $strand,
+ -SEQ_REGION_NAME => $seq_region,
+ -COORD_SYSTEM => $cs,
+ -ADAPTOR => $adaptor,
+ -REPEAT_MASK => ['repeat_masker'],
+ -SOFT_MASK => 1);
+ Description: Creates a Slice which behaves exactly as a normal slice but
+ that returns repeat masked sequence from the seq method.
+ Returntype : Bio::EnsEMBL::RepeatMaskedSlice
+ Exceptions : none
+ Caller : RawComputes (PredictionTranscript creation code).
+
+=cut
+
+sub new {
+ my $caller = shift;
+ my $class = ref($caller) || $caller;
+
+ my ($logic_names, $soft_mask) = rearrange(['REPEAT_MASK',
+ 'SOFT_MASK'], @_);
+
+ my $self = $class->SUPER::new(@_);
+
+
+ $logic_names ||= [''];
+ if(ref($logic_names) ne 'ARRAY') {
+ throw("Reference to list of logic names argument expected.");
+ }
+
+ $self->{'repeat_mask_logic_names'} = $logic_names;
+ $self->{'soft_mask'} = $soft_mask;
+
+ return $self;
+}
+
+
+=head2 repeat_mask_logic_names
+
+ Arg [1] : reference to list of strings $logic_names (optional)
+ Example : $rm_slice->repeat_mask_logic_name(['repeat_masker']);
+ Description: Getter/Setter for the logic_names of the repeats that are used
+ to mask this slices sequence.
+ Returntype : reference to list of strings
+ Exceptions : none
+ Caller : seq() method
+
+=cut
+
+sub repeat_mask_logic_names {
+ my $self = shift;
+
+ if(@_) {
+ my $array = shift;
+ if(ref($array) ne 'ARRAY') {
+ throw('Reference to list of logic names argument expected.');
+ }
+ }
+
+ return $self->{'repeat_mask_logic_names'};
+}
+
+
+=head2 soft_mask
+
+ Arg [1] : boolean $soft_mask (optional)
+ Example : $rm_slice->soft_mask(0);
+ Description: Getter/Setter which is used to turn on/off softmasking of the
+ sequence returned by seq.
+ Returntype : boolean
+ Exceptions : none
+ Caller : seq() method
+
+=cut
+
+sub soft_mask {
+ my $self = shift;
+ $self->{'soft_mask'} = shift if(@_);
+ return $self->{'soft_mask'} || 0;
+}
+
+
+
+=head2 seq
+
+ Arg [1] : none
+ Example : print $rmslice->seq(), "\n";
+ Description: Retrieves the entire repeat masked sequence for this slice.
+ See also the B<Bio::EnsEMBL::Slice> implementation of this
+ method.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub seq {
+ my $self = shift;
+ #
+ # get all the features
+ #
+ my $logic_names = $self->repeat_mask_logic_names();
+ my $soft_mask = $self->soft_mask();
+
+ my $repeats = [];
+
+ foreach my $l (@$logic_names) {
+ push @{$repeats}, @{$self->get_all_RepeatFeatures($l)};
+ }
+
+ #
+ # get the dna
+ #
+ my $dna = $self->SUPER::seq(@_);
+
+ #
+ # mask the dna
+ #
+ $self->_mask_features(\$dna,$repeats,$soft_mask);
+ return $dna;
+}
+
+
+
+=head2 subseq
+
+ Arg [1] : none
+ Example : print $rmslice->subseq(1, 1000);
+ Description: Retrieves a repeat masked sequence from a specified subregion
+ of this slice. See also the B<Bio::EnsEMBL::Slice>
+ implementation of this method.
+ Returntype : string
+ Exceptions : none
+ Caller : general
+
+=cut
+
+
+sub subseq {
+ my $self = shift;
+ #
+ # get all the features
+ #
+ my $logic_names = $self->repeat_mask_logic_names();
+ my $soft_mask = $self->soft_mask();
+
+ my $repeats = [];
+
+ foreach my $l (@$logic_names) {
+ push @{$repeats}, @{$self->get_all_RepeatFeatures($l)};
+ }
+
+ #
+ # get the dna
+ #
+ my $dna = $self->SUPER::subseq(@_);
+
+ #
+ # mask the dna
+ #
+ $self->_mask_features(\$dna,$repeats,$soft_mask);
+ return $dna;
+}
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/Root.pm b/modules/Bio/EnsEMBL/Root.pm
index 5ba84946ac..d7b1619bf0 100644
--- a/modules/Bio/EnsEMBL/Root.pm
+++ b/modules/Bio/EnsEMBL/Root.pm
@@ -2,28 +2,29 @@
Bio::EnsEMBL::Root
-=head1 SYNOPSIS
+=head1 DESCRIPTION
- # any bioperl or bioperl compliant object is a RootI
- # compliant object
+Do not use Bio::EnsEMBL::Root anymore. It is included for backwards
+compatibility (every object in EnsEMBL used to inherit from this class) but
+will eventually be phased out. The replacement for the _rearrage method is the
+rearrange method which can be imported in the following way:
- $obj->throw("This is an exception");
+ use Bio::EnsEMBL::Utils::Argument qw(rearrange);
- eval {
- $obj->throw("This is catching an exception");
- };
+ #can now call rearrange as a class method (instead as object method)
+ my ($start, $end) = rearrange(['START','END'], \@args);
- if( $@ ) {
- print "Caught exception";
- } else {
- print "no exception";
- }
+If you want to use the throw or warn methods the replacement are the class
+methods throw and warning from the Bio::EnsEMBL::Utils::Exception class:
+
+ use Bio::EnsEMBL::Utils::Exception qw(throw warning);
+
+ #can now call throw or warning even without bless reference
+ warning('This is a warning');
+ throw('This is an exception');
-=head1 DESCRIPTION
-This package provides a bare-bones root class for Ensembl objects to
-inherit from.
-Shamelessly stolen from BioPerl to avoid problems with moving to BioPerl 1
+This module was stolen from BioPerl to avoid problems with moving to BioPerl 1
from 0.7
=head1 CONTACT
diff --git a/modules/Bio/EnsEMBL/SNP.pm b/modules/Bio/EnsEMBL/SNP.pm
index 9cc4a04fe4..513c058977 100644
--- a/modules/Bio/EnsEMBL/SNP.pm
+++ b/modules/Bio/EnsEMBL/SNP.pm
@@ -113,16 +113,6 @@ sub end {
return $self->{_gsf_end};
}
-sub chr_name {
- my ($self, $arg) = @_;
-
- if(defined $arg) {
- $self->{_chr_name} = $arg;
- }
-
- return $self->{_chr_name};
-}
-
sub source {
my ($self, $arg) = @_;
@@ -156,4 +146,25 @@ sub source_tag {
}
+
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $snp->id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For snps this is the
+ returns the same thing as the id method.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
+
+=cut
+
+sub display_id {
+ my $self = shift;
+ return $self->id();
+}
+
+
+
1;
diff --git a/modules/Bio/EnsEMBL/SeqFeature.pm b/modules/Bio/EnsEMBL/SeqFeature.pm
index 6af78c3bd3..195d64c65c 100755
--- a/modules/Bio/EnsEMBL/SeqFeature.pm
+++ b/modules/Bio/EnsEMBL/SeqFeature.pm
@@ -13,42 +13,33 @@
Bio::EnsEMBL::SeqFeature - Ensembl specific sequence feature.
-=head1 SYNOPSIS
-
- my $feat = new Bio::EnsEMBL::SeqFeature(-seqname => 'pog',
- -start => 100,
- -end => 220,
- -strand => -1,
- -frame => 1,
- -source_tag => 'tblastn_vert',
- -primary_tag => 'similarity',
- -analysis => $analysis
- );
-
- # $analysis is a Bio::EnsEMBL::Analysis object
-
- # SeqFeatureI methods can be used
- my $start = $feat->start;
- my $end = $feat->end;
+=head1 DESCRIPTION
- # Bio::EnsEMBL::SeqFeature specific methods can be used
- my $analysis = $feat->analysis;
+Do not use this module if you can avoid it. It has been replaced by
+Bio::EnsEMBL::Feature. This module has a long history of usage but has
+become very bloated, and quite unweildy. It was decided to replace it
+completely with a smaller, light-weight feature class rather than attempting
+to refactor this class, and maintain strict backwards compatibility.
- # Validate all the data in the object
- $feat->validate || $feat->throw("Invalid data in $feat");
+Part of the complexity of this class was in its extensive inheritance. As an
+example the following is a simplified inheritance heirarchy that was present
+for Bio::EnsEMBL::DnaAlignFeature:
-=head1 DESCRIPTION
+Bio::EnsEMBL::DnaAlignFeature
+Bio::EnsEMBL::BaseAlignFeature
+Bio::EnsEMBL::FeaturePair
+Bio::EnsEMBL::SeqFeature
+Bio::EnsEMBL::SeqFeatureI
+Bio::SeqFeatureI
+Bio::RangeI
+Bio::Root::RootI
-This is an implementation of the ensembl Bio::EnsEMBL::SeqFeatureI interface.
-Extra
-methods are to store details of the analysis program/database/version used
-to create this data and also a method to validate all data in the object is
-present and of the right type. This is useful before writing into
-a relational database for example.
+The new Bio::EnsEMBL::Feature class is much shorter, and hopefully much easier
+to understand and maintain.
=head1 CONTACT
-Describe contact details here
+Post questions to the ensembl development list: ensembl-dev@ebi.ac.uk
=head1 APPENDIX
diff --git a/modules/Bio/EnsEMBL/SeqFeatureI.pm b/modules/Bio/EnsEMBL/SeqFeatureI.pm
index 0b6e739b93..bdf7c0fb71 100755
--- a/modules/Bio/EnsEMBL/SeqFeatureI.pm
+++ b/modules/Bio/EnsEMBL/SeqFeatureI.pm
@@ -12,33 +12,16 @@
=head1 NAME
-Bio::EnsEMBL::SeqFeatureI - Ensembl specific sequence feature interface.
-
-=head1 SYNOPSIS
-
- my $feat = get_feature_from_somewhere;
-
- # Bio::SeqFeatureI methods can be used
- my $start = $feat->start;
- my $end = $feat->end;
-
- # New Bio::EnsEMBL::SeqFeatureI specific methods are :
- my $analysis = $feat->analysis;
-
- # Validate all the data in the object
- $feat->validate || $feat->throw("Invalid data in $feat");
+Bio::EnsEMBL::SeqFeatureI
=head1 DESCRIPTION
-This is an extension of the bioperl Bio::SeqFeatureI interface. Extra
-methods are to store details of the analysis program/database/version used
-to create this data and also a method to validate all data in the object is
-present and of the right type. This is useful before writing into
-a relational database for example.
+Do not use this class. It is deprecated and has been replaced by
+Bio::EnsEMBL::Feature.
=head1 CONTACT
-Describe contact details here
+Post questions to the ensembl development mailing list: ensembl-dev@ebi.ac.uk
=head1 APPENDIX
diff --git a/modules/Bio/EnsEMBL/SimpleFeature.pm b/modules/Bio/EnsEMBL/SimpleFeature.pm
index c12d8cae30..29ce41214a 100644
--- a/modules/Bio/EnsEMBL/SimpleFeature.pm
+++ b/modules/Bio/EnsEMBL/SimpleFeature.pm
@@ -1,9 +1,6 @@
-
#
# Ensembl module for Bio::EnsEMBL::SimpleFeature
#
-# Cared for by Ewan Birney <birney@ebi.ac.uk>
-#
# Copyright Ewan Birney
#
# You may distribute this module under the same terms as perl itself
@@ -12,97 +9,154 @@
=head1 NAME
-Bio::EnsEMBL::SimpleFeature - DESCRIPTION of Object
+Bio::EnsEMBL::SimpleFeature - A simple feature with a location and label
=head1 SYNOPSIS
-Give standard usage here
+use Bio::EnsEMBL::SimpleFeature;
+
+$feature = Bio::EnsEMBL::SimpleFeature->new(-start => 100,
+ -end => 220,
+ -strand => -1,
+ -slice => $slice,
+ -analysis => $analysis,
+ -score => 58,
+ -display_label => 'EponineTSS',
+ -dbID => 1230,
+ -adaptor => $adaptor);
=head1 DESCRIPTION
-Describe the object here
+This is a simple feature which extends the Feature class to add display_label
+and score attributes.
=head1 AUTHOR - Ewan Birney
This modules is part of the Ensembl project http://www.ensembl.org
-Email birney@ebi.ac.uk
-
-Describe contact details here
+=head1 CONTACT
-=head1 APPENDIX
+Post comments/questions to the ensembl development list: ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
+=head1 METHODS
=cut
-
-# Let the code begin...
-
+use strict;
package Bio::EnsEMBL::SimpleFeature;
+
use vars qw(@ISA);
-use strict;
-# Object preamble - inherits from Bio::EnsEMBL::Root
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+@ISA = qw(Bio::EnsEMBL::Feature);
+
+
+=head2 new
+
+ Arg [DISPLAY_LABEL]: The label assigned to this simple feature
+ Arg [...] : Named arguments passed to superclass
+ Example : $feature = Bio::EnsEMBL::SimpleFeature->new
+ (-start => 1,
+ -end => 100,
+ -strand => 1,
+ -slice => $slice,
+ -analysis => $analysis,
+ -adaptor => $adaptor,
+ -dbID => 10,
+ -display_label => 'EponineTSS',
+ -score => 100);
+ Description: Constructs a new Bio::EnsEMBL::Feature. Generally subclasses
+ of this method are instantiated, rather than this class itself.
+ Returntype : Bio::EnsEMBL::Feature
+ Exceptions : Thrown on invalid -SLICE, -ANALYSIS, -STRAND arguments
+ Caller : general, subclass constructors
+
+=cut
-use Bio::EnsEMBL::SeqFeature;
+sub new {
+ my $caller = shift;
-@ISA = qw(Bio::EnsEMBL::SeqFeature);
+ #allow this to be called as class or object method
+ my $class = ref($caller) || $caller;
+ my $self = $class->SUPER::new(@_);
-# new() is inherieted from SeqFeature
+ my ($display_label, $score) = rearrange(['DISPLAY_LABEL','SCORE'],@_);
+
+ $self->{'display_label'} = $display_label;
+ $self->{'score'} = $score;
+
+ return $self;
+}
+
+
+sub new_fast {
+ my $class = shift;
+ my $hashref = shift;
+ return bless $hashref, $class;
+}
=head2 display_label
- Title : display_label
- Usage : $obj->display_label($newval)
- Function:
- Example :
- Returns : value of display_label
- Args : newvalue (optional)
+ Arg [1] : (optional) string $value
+ Example : $label = $simple_feature->display_label();
+ Description: Getter/Setter for the display label associated with this
+ feature.
+ Returntype : string
+ Exceptions : none
+ Caller : general
=cut
sub display_label{
- my ($self, $value) = @_;
+ my $self = shift;
- if (defined $value) {
- $self->{'display_label'} = $value;
- }
- return $self->{'display_label'};
+ $self->{'display_label'} = shift if(@_);
+
+ return $self->{'display_label'};
}
-sub dbID{
- my ($self, $arg) = @_;
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $rf->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For simple features this is the
+ display_label if it is available otherwise it is an empty
+ string.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
- if(defined $arg) {
- $self->{_database_id} = $arg;
- }
- return $self->{_database_id};
+=cut
+
+sub display_id {
+ my $self = shift;
+ return $self->{'display_label'} || '';
}
-=head2 adaptor
- Arg [1] : Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor $adaptor
- Example : none
- Description: get/set for this objects Adaptor
- Returntype : Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor
+=head2 score
+
+ Arg [1] : (optional) string $value
+ Example : $score = $simple_feature->score();
+ Description: Getter/Setter for the score associated with this
+ feature.
+ Returntype : string
Exceptions : none
- Caller : general, set from adaptor on store
+ Caller : general
=cut
-sub adaptor {
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'adaptor'} = $value;
- }
- return $self->{'adaptor'};
-
+sub score {
+ my $self = shift;
+ $self->{'score'} = shift if(@_);
+ return $self->{'score'};
}
diff --git a/modules/Bio/EnsEMBL/Slice.pm b/modules/Bio/EnsEMBL/Slice.pm
index bf1da683d2..42e5c6d293 100644
--- a/modules/Bio/EnsEMBL/Slice.pm
+++ b/modules/Bio/EnsEMBL/Slice.pm
@@ -1,7 +1,6 @@
#
-# Ensembl module for Bio::EnsEMBL::Assembly::Slice
+# Ensembl module for Bio::EnsEMBL::Slice
#
-# Cared for by Ewan Birney <ensembl-dev@ebi.ac.uk>
#
# Copyright Ewan Birney
#
@@ -15,18 +14,34 @@ Bio::EnsEMBL::Slice - Arbitary Slice of a genome
=head1 SYNOPSIS
- $slice_adaptor = $db->get_SliceAdaptor;
+ $sa = $db->get_SliceAdaptor;
- $slice = $slice_adaptor->fetch_by_chr_start_end('X', 1_000_000, 2_000_000);
+ $slice = $sa->fetch_by_region('chromosome', 'X', 1_000_000, 2_000_000);
+ #get some attributes of the slice
+ my $seqname = $slice->seq_region_name();
+ my $start = $slice->start();
+ my $end = $slice->end();
+ print "$seqname, $start-$end\n";
+
+ #get the sequence from the slice
+ my $seq = $slice->seq();
+ print $seq;
+
+ #get some features from the slice
foreach $gene ( @{$slice->get_all_Genes} ) {
# do something with a gene
}
-
-=head1 DESCRIPTION
+ foreach my $feature ( @{$slice->get_all_DnaAlignFeatures}) {
+ # do something with dna-dna alignments
+ }
+=head1 DESCRIPTION
+
+A Slice object represents a region of a genome. It can be used to retrieve
+sequence or features from an area of interest.
=head1 AUTHOR - Ewan Birney
@@ -37,39 +52,58 @@ This modules is part of the Ensembl project http://www.ensembl.org
Questions can be posted to the ensembl-dev mailing list:
ensembl-dev@ebi.ac.uk
+=head1 METHODS
+
=cut
package Bio::EnsEMBL::Slice;
use vars qw(@ISA);
use strict;
-use Bio::EnsEMBL::Root;
-use Bio::PrimarySeqI;
-use Bio::EnsEMBL::Tile;
+use Bio::EnsEMBL::Root; #included for backwards compatibility
+use Bio::EnsEMBL::Tile; #included for backwards compatibility
+use Bio::EnsEMBL::Chromosome; #included for backwards compatibility
+use Bio::EnsEMBL::RawContig; #included for backwards compatibility
+use Bio::PrimarySeqI;# included only for backwards compatibility
+
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+use Bio::EnsEMBL::Utils::Exception qw(throw deprecate warning);
+use Bio::EnsEMBL::RepeatMaskedSlice;
+
+#inheritance to Bio::EnsEMBL::Root will eventually be removed
@ISA = qw(Bio::EnsEMBL::Root Bio::PrimarySeqI);
=head2 new
- Arg [...] : List of optional named arguments
- string CHR_NAME,
- int CHR_START,
- int CHR_END,
- int STRAND,
- string ASSEMBLY_TYPE,
- Bio::EnsEMBL::DBSQL::SliceAdaptor ADAPTOR
- int DBID
- boolean EMPTY
- Example : $slice = new Bio::EnsEMBL::Slice(-start => 1,
- -end => 10000,
- -chr_name => 'X',
- -adaptor => $slice_adaptor);
- Description: Creates a new slice object. The empty flag is intended to
- create an empty slice which is not on a particular chromosome.
- In this way objects can be transformed to slice coordinates
- from raw contig coordinates when their location in the assembly
- is not known.
+ Arg [...] : List of named arguments
+ string COORD_SYSTEM
+ string SEQ_REGION_NAME,
+ int START,
+ int END,
+ string VERSION (optional, defaults to '')
+ int STRAND, (optional, defaults to 1)
+ Bio::EnsEMBL::DBSQL::SliceAdaptor ADAPTOR (optional)
+ Example : $slice = Bio::EnsEMBL::Slice->new(-coord_system => $cs,
+ -start => 1,
+ -end => 10000,
+ -strand => 1,
+ -seq_region_name => 'X',
+ -adaptor => $slice_adaptor);
+ Description: Creates a new slice object. A slice represents a region
+ of sequence in a particular coordinate system. Slices can be
+ used to retrieve sequence and features from an area of
+ interest in a genome.
+
+ Coordinates start at 1 and are inclusive. Negative
+ coordinates or coordinates exceeding the length of the
+ seq_region are permitted. Start must be less than or equal.
+ to end regardless of the strand.
+
+ Slice objects are immutable. Once instantiated their attributes
+ (with the exception of the adaptor) may not be altered. To
+ change the attributes a new slice must be created.
Returntype : Bio::EnsEMBL::Slice
Exceptions : none
Caller : general, Bio::EnsEMBL::SliceAdaptor
@@ -77,58 +111,57 @@ use Bio::EnsEMBL::Tile;
=cut
sub new {
- my($class,@args) = @_;
-
- my $self = {};
- bless $self,$class;
-
- my ($chr,$start,$end,$strand,$type,$adaptor, $dbID, $empty) =
- $self->_rearrange([qw(CHR_NAME
- CHR_START
- CHR_END
- STRAND
- ASSEMBLY_TYPE
- ADAPTOR
- DBID
- EMPTY)],
- @args);
-
- if( ! defined $empty ) {
- if( !defined $chr || !defined $start || !defined $end || !defined $type ) {
- #warn "Chr: $chr\tStart: $start\tEnd: $end\tType: $type";
- $self->throw("Do not have all the parameters for slice");
- }
- $self->chr_name($chr);
- $self->chr_start($start);
- $self->chr_end($end);
+ my $caller = shift;
- if(!defined $strand) {
- $strand = 1; #default slice strand is 1
- } else {
- unless($strand == 1 || $strand == -1) {
- $self->throw("Slice strand must be either -1 or 1 not [$strand].");
- }
- }
- $self->strand($strand);
- } else {
- $self->strand( 1 );
- $self->chr_start( 1 );
-
- # empty Slices are used to do mapping to chromosomal coords.
- # After the mapping, the Slice contains chr_name and is reference
- # point for the mapped object
+ #new can be called as a class or object method
+ my $class = ref($caller) || $caller;
+
+ my ($seq, $coord_system, $seq_region_name,
+ $start, $end, $strand, $adaptor, $empty) =
+ rearrange([qw(SEQ COORD_SYSTEM SEQ_REGION_NAME
+ START END STRAND ADAPTOR EMPTY)], @_);
+
+ #empty is only for backwards compatibility
+ if($empty) {
+ deprecate("Creation of empty slices is no longer needed" .
+ "and is deprecated");
+ return bless({'empty' => 1, 'adaptor' => $adaptor}, $class);
+ }
+
+ $seq_region_name || throw('SEQ_REGION_NAME argument is required');
+ defined($start) || throw('START argument is required');
+ defined($end) || throw('END argument is required');
+ ($start <= $end) || throw('start must be less than or equal to end');
+
+ if($seq && length($seq) != ($end - $start + 1)){
+ throw('SEQ must be the same length as the defined LENGTH not '.
+ length($seq).' compared to '.($end - $start + 1));
}
+ if(!$coord_system || !ref($coord_system) ||
+ !$coord_system->isa('Bio::EnsEMBL::CoordSystem')) {
+ throw('COORD_SYSTEM argment must be a Bio::EnsEMBL::CoordSystem');
+ }
+
+ #strand defaults to 1 if not defined
+ $strand ||= 1;
- $self->assembly_type($type);
- $self->adaptor($adaptor);
- $self->dbID( $dbID );
- # set stuff in self from @args
+ if($strand != 1 && $strand != -1) {
+ throw('STRAND argument must be -1 or 1');
+ }
- if( defined $adaptor && !defined $type ) {
- $self->assembly_type
- ( $adaptor->db()->get_MetaContainer()->get_default_assembly());
+ if(defined($adaptor)) {
+ if(!ref($adaptor) || !$adaptor->isa('Bio::EnsEMBL::DBSQL::SliceAdaptor')) {
+ throw('ADAPTOR argument must be a Bio::EnsEMBL::DBSQL::SliceAdaptor');
+ }
}
- return $self;
+
+ return bless {'coord_system' => $coord_system,
+ 'seq' => $seq,
+ 'seq_region_name' => $seq_region_name,
+ 'start' => $start,
+ 'end' => $end,
+ 'strand' => $strand,
+ 'adaptor' => $adaptor}, $class;
}
@@ -146,163 +179,158 @@ sub new {
=cut
sub adaptor{
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'adaptor'} = $value;
- }
- return $self->{'adaptor'};
+ my $self = shift;
+
+ if(@_) {
+ my $ad = shift;
+ if(defined($ad)) {
+ if(!ref($ad) || !$ad->isa('Bio::EnsEMBL::DBSQL::SliceAdaptor')) {
+ throw('Argument must be a Bio::EnsEMBL::DBSQL::SliceAdaptor');
+ }
+ }
+ $self->{'adaptor'} = $ad;
+ }
+
+ return $self->{'adaptor'};
}
-=head2 dbID
+=head2 seq_region_name
- Arg [1] : (optioanl) int $value
- Example : none
- Description: Getter/Setter for the unique database identifier for this
- slice. This is not currently useful since slices are
- abstractions and not actually stored in a database. This
- function is present to mirror RawContigs dbID method and
- because it could in theory be used one day.
- Returntype : int
+ Arg [1] : none
+ Example : $seq_region = $slice->seq_region_name;
+ Description: Returns the name of the seq_region that this slice is on. For
+ example if this slice is in chromosomal coordinates the
+ seq_region_name might be 'X' or '10'.
+
+ This function was formerly named chr_name, but since slices can
+ now be on coordinate systems other than chromosomal it has been
+ changed.
+ Returntype : string
Exceptions : none
- Caller : none
+ Caller : general
=cut
-sub dbID {
- my ( $self, $value ) = @_;
- if( defined $value ) {
- $self->{'dbID'} = $value;
- }
- return $self->{'dbID'};
+sub seq_region_name {
+ my $self = shift;
+ return $self->{'seq_region_name'};
}
-=head2 name
+=head2 coord_system
- Arg [1] : optional string $name
- Example : $name = $slice->name();
- Description: Returns the name of this slice. The name is formatted as a
- the following string: "$chr_name.$chr_start-$chr_end".
- (e.g. 'X.10000-20000')
- This essentially allows slices to be easily compared and
- can also act as a hash value. This is similar to the name
- method in RawContig so for exons which can have either type
- of sequence attached it provides a more common interface.
- You can as well set the slicename to something like "NT_110023"
- Returntype : string
+ Arg [1] : none
+ Example : print $slice->coord_system->name();
+ Description: Returns the coordinate system that this slice is on.
+ Returntype : Bio::EnsEMBL::CoordSystem
Exceptions : none
Caller : general
=cut
-sub name {
- my ( $self, $arg ) = @_;
-
- if( defined $arg ) {
- $self->{name} = $arg;
- } elsif(!defined $self->{name}) {
- my $chr_name = $self->chr_name || '';
- my $chr_start = $self->chr_start || '';
- my $chr_end = $self->chr_end || '';
-
- my $string = "$chr_name.$chr_start-$chr_end";
-
- if($self->strand == -1) {
- $self->{name} = "reverse($string)";
- } else {
- $self->{name} = $string;
- }
- }
-
- return $self->{name};
+sub coord_system {
+ my $self = shift;
+ return $self->{'coord_system'};
}
-=head2 get_all_QtlFeatures
- Args : none
- Example : none
- Description: returns overlapping QtlFeatures
- Returntype : listref Bio::EnsEMBL::Map::QtlFeature
+=head2 start
+
+ Arg [1] : none
+ Example : $start = $slice->start();
+ Description: Returns the start position of this slice relative to the
+ start of the sequence region that it was created on.
+ Coordinates are inclusive and start at 1. Negative coordinates
+ or coordinates exceeding the length of the sequence region are
+ permitted. Start is always less than or equal to end
+ regardless of the orientation of the slice.
+ Returntype : int
Exceptions : none
Caller : general
=cut
-sub get_all_QtlFeatures {
+sub start {
my $self = shift;
+ return $self->{'start'};
+}
- my $qfAdaptor;
- if( $self->adaptor()) {
- $qfAdaptor = $self->adaptor()->db()->get_QtlFeatureAdaptor();
- } else {
- return [];
- }
- return $qfAdaptor->fetch_all_by_Slice_constraint( $self );
+
+=head2 end
+
+ Arg [1] : none
+ Example : $end = $slice->end();
+ Description: Returns the end position of this slice relative to the
+ start of the sequence region that it was created on.
+ Coordinates are inclusive and start at 1. Negative coordinates
+ or coordinates exceeding the length of the sequence region are
+ permitted. End is always greater than or equal to start
+ regardless of the orientation of the slice.
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub end {
+ my $self = shift;
+ return $self->{'end'};
}
-=head2 get_all_supercontig_Slices
+
+=head2 strand
Arg [1] : none
- Example : none
- Description: Returns Slices that represent overlapping supercontigs.
- Coordinates inside those slices are supercontig coordinates.
- You can transfer features to this slices coordinate system with
- the normal transform call. The returned slices hav their names
- set to the supercontig names.
- Returntype : listref Bio::EnsEMBL::Slice
+ Example : $strand = $slice->strand();
+ Description: Returns the orientation of this slice on the seq_region it has
+ been created on
+ Returntype : int (either 1 or -1)
Exceptions : none
- Caller : none
+ Caller : general, invert
=cut
-
-sub get_all_supercontig_Slices {
+sub strand{
my $self = shift;
- my $result = [];
+ return $self->{'strand'};
+}
- if( $self->adaptor() ) {
- my $superctg_names = $self->adaptor()->list_overlapping_supercontigs( $self );
-
- for my $name ( @$superctg_names ) {
- my $slice;
- $slice = $self->adaptor()->fetch_by_supercontig_name( $name );
- $slice->name( $name );
- push( @$result, $slice );
- }
- } else {
- $self->warn( "Slice needs to be attached to a database to get supercontigs" );
- }
- return $result;
-}
-
+=head2 name
-=head2 id
+ Arg [1] : none
+ Example : my $results = $cache{$slice->name()};
+ Description: Returns the name of this slice. The name is formatted as a colon
+ delimited string with the following attributes:
+ coord_system:version:seq_region_name:start:end:strand
- Arg [1] : none
- Example : none
- Description: Here to mirror same method in RawContig. Simply returns
- the same thing as $slice->name() and generally name should be
- used instead.
+ Slices with the same name are equivalent and thus the name can
+ act as a hash key.
Returntype : string
Exceptions : none
- Caller : none
+ Caller : general
=cut
-sub id {
- my $self = shift;
+sub name {
+ my $self = shift;
- return $self->name() || $self->dbID();
+ return join(':',
+ $self->{'coord_system'}->name(),
+ $self->{'coord_system'}->version(),
+ $self->{'seq_region_name'},
+ $self->{'start'},
+ $self->{'end'},
+ $self->{'strand'});
}
@@ -321,7 +349,7 @@ sub id {
sub length {
my ($self) = @_;
- return $self->chr_end() - $self->chr_start() + 1;
+ return $self->{'end'} - $self->{'start'} + 1;
}
@@ -330,7 +358,7 @@ sub length {
Arg [1] : none
Example : $inverted_slice = $slice->invert;
- Description: Creates a copy of this slice on the opposite strand and
+ Description: Creates a copy of this slice on the opposite strand and
returns it.
Returntype : Bio::EnsEMBL::Slice
Exceptions : none
@@ -340,73 +368,85 @@ sub length {
sub invert {
my $self = shift;
-
+
+ #make a shallow copy of the slice via a hash copy,
my %s = %$self;
- my $slice = bless \%s, ref $self;
- $slice->strand($self->strand * -1);
- delete $slice->{name};
-
- return $slice;
+ if(!$self->adaptor){
+ warning("You can't invert a Slice which isn't attached to a database");
+ return;
+ }
+ #flip the strand,
+ $s{'strand'} = $self->{'strand'} * -1;
+ #bless and return the copy
+ return bless \%s, ref $self;
}
+
=head2 seq
- Args : none
- Function : Returns the entire sequence string for this Slice. This method
- will return the reverse complement of the sequence of another
- slice on the same region but on the opposite strand.
- Note that the slice needs the adaptor to be set to obtain the
- sequence.
- Returntype: txt
- Exceptions: none
- Caller : general
+ Arg [1] : none
+ Example : print "SEQUENCE = ", $slice->seq();
+ Description: Returns the sequence of the region represented by this
+ slice formatted as a string.
+ Returntype : string
+ Exceptions : none
+ Caller : general
=cut
sub seq {
my $self = shift;
- my $seqAdaptor = $self->adaptor->db->get_SequenceAdaptor();
- return $seqAdaptor->fetch_by_Slice_start_end_strand( $self, 1, -1, 1 );
+
+ if(!$self->{'seq'}){
+ my $seqAdaptor = $self->adaptor->db->get_SequenceAdaptor;
+ return ${$seqAdaptor->fetch_by_Slice_start_end_strand( $self, 1,undef, 1 )};
+ }
+
+ return $self->{'seq'};
}
=head2 subseq
- Arg 1 : int $startBasePair
- relative to start of slice, which is 1.
- Arg 2 : int $endBasePair
- relative to start of slice.
- Arg 3 : (optional) int $strand
- The strand of the slice to obtain sequence from. Default value is
- 1.
- Function : returns string of dna sequence
- Returntype: txt
- Exceptions: end should be at least as big as start
- strand must be set
- Caller : general
+ Arg [1] : int $startBasePair
+ relative to start of slice, which is 1.
+ Arg [2] : int $endBasePair
+ relative to start of slice.
+ Arg [3] : (optional) int $strand
+ The strand of the slice to obtain sequence from. Default
+ value is 1.
+ Description: returns string of dna sequence
+ Returntype : txt
+ Exceptions : end should be at least as big as start
+ strand must be set
+ Caller : general
=cut
sub subseq {
my ( $self, $start, $end, $strand ) = @_;
-
+
if ( $end < $start ) {
- $self->throw("End coord is less then start coord");
+ throw("End coord is less then start coord");
}
$strand = 1 unless(defined $strand);
if ( $strand != -1 && $strand != 1 ) {
- $self->throw("Invalid strand [$strand] in call to Slice::subseq.");
+ throw("Invalid strand [$strand] in call to Slice::subseq.");
}
-
- my $seqAdaptor = $self->adaptor->db->get_SequenceAdaptor();
- my $seq = $seqAdaptor->fetch_by_Slice_start_end_strand( $self, $start,
- $end, $strand );
-
- return $seq;
+ my $subseq;
+ if($self->adaptor){
+ my $seqAdaptor = $self->adaptor->db->get_SequenceAdaptor();
+ $subseq = ${$seqAdaptor->fetch_by_Slice_start_end_strand
+ ( $self, $start,
+ $end, $strand )};
+ }else{
+ $subseq = substr ($self->seq(), ($start-1), $end);
+ }
+ return $subseq;
}
@@ -423,8 +463,8 @@ sub subseq {
'g' => num,
'n' => num,
'%gc' => num }
-
- All bases which are not in the set [A,a,C,c,T,t,G,g] are
+
+ All bases which are not in the set [A,a,C,c,T,t,G,g] are
included in the 'n' count. The 'n' count could therefore be
inclusive of ambiguity codes such as 'y'.
The %gc is the ratio of GC to AT content as in:
@@ -450,18 +490,16 @@ sub get_base_count {
while($start <= $len) {
$end = $start + $RANGE - 1;
-
+
$end = $len if($end > $len);
-
$seq = $self->subseq($start, $end);
-
$a += $seq =~ tr/Aa/Aa/;
$c += $seq =~ tr/Cc/Cc/;
$t += $seq =~ tr/Tt/Tt/;
$g += $seq =~ tr/Gg/Gg/;
$start = $end + 1;
- }
+ }
my $gc_content = 0;
if($a || $g || $c || $t) { #avoid divide by 0
@@ -478,6 +516,305 @@ sub get_base_count {
+=head2 project
+
+ Arg [1] : string $name
+ The name of the coordinate system to project this slice onto
+ Arg [2] : string $version
+ The version of the coordinate system (such as 'NCBI34') to
+ project this slice onto
+ Example :
+ my $clone_projection = $slice->project('clone');
+
+ foreach my $segment (@$clone_projection) {
+ my ($start, $end, $clone) = @$segment;
+ print $slice->seq_region_name, ':', $start, '-', $end , ' -> ',
+ $clone->seq_region_name, ':', $clone->start, '-', $clone->end,
+ $clone->strand, "\n";
+ }
+ Description: Returns the results of 'projecting' this slice onto another
+ coordinate system. Projecting to a coordinate system that
+ the slice is assembled from is analagous to retrieving a tiling
+ path. This method may also be used to 'project up' to a higher
+ level coordinate system, however.
+
+ This method returns a listref of triplets [start,end,slice]
+ which represents the projection. The start and end defined the
+ region of this slice which is made up of the third value of
+ the triplet: a slice in the requested coordinate system.
+ Returntype : list reference of [$start,$end,$slice] triplets
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub project {
+ my $self = shift;
+ my $cs_name = shift;
+ my $cs_version = shift;
+
+ throw('Coord_system name argument is required') if(!$cs_name);
+
+ #obtain a mapper between this coordinate system and the requested one
+ if(!$self->adaptor){
+ warning("You can't project a slice which isn't attached to a ".
+ "database on to another coordinate system");
+ return;
+ }
+ my $slice_adaptor = $self->adaptor();
+
+ if(!$slice_adaptor) {
+ warning("Cannot project slice without an attached SliceAdaptor");
+ return [];
+ }
+
+ my $db = $slice_adaptor->db();
+ my $csa = $db->get_CoordSystemAdaptor();
+ my $cs = $csa->fetch_by_name($cs_name, $cs_version);
+ my $slice_cs = $self->coord_system();
+
+ #no mapping is needed if the requested coord system is the one we are in
+ #but we do need to check if some of the slice is outside of defined regions
+ if($slice_cs->equals($cs)) {
+ my $entire_slice = $slice_adaptor->fetch_by_region($cs->name(),
+ $self->{'seq_region_name'},
+ undef, undef, undef,
+ $cs->version());
+
+ my $entire_len = $entire_slice->length();
+
+ #if the slice has negative coordinates or coordinates exceeding the
+ #exceeding length of the sequence region we want to shrink the slice to
+ #the defined region
+
+ if($self->{'start'} > $entire_len || $self->{'end'} < 1) {
+ #none of this slice is in a defined region
+ return [];
+ }
+
+ my $right_contract = 0;
+ my $left_contract = 0;
+ if($self->{'end'} > $entire_len) {
+ $right_contract = $entire_len - $self->{'end'};
+ }
+ if($self->{'start'} < 1) {
+ $left_contract = $self->{'start'} - 1;
+ }
+
+ my $new_slice;
+ if($left_contract || $right_contract) {
+ $new_slice = $self->expand($left_contract, $right_contract);
+ } else {
+ $new_slice = $self;
+ }
+
+ return [[1-$left_contract, $self->length()+$right_contract, $new_slice]];
+ }
+
+ my @projection;
+ my $current_start = 1;
+
+ #decompose this slice into its symlinked components.
+ #this allows us to handle haplotypes and PARs
+ my $normal_slice_proj =
+ $slice_adaptor->fetch_normalized_slice_projection($self);
+ foreach my $segment (@$normal_slice_proj) {
+ my $normal_slice = $segment->[2];
+
+ $slice_cs = $normal_slice->coord_system();
+
+ my $asma = $db->get_AssemblyMapperAdaptor();
+ my $asm_mapper = $asma->fetch_by_CoordSystems($slice_cs, $cs);
+
+ # perform the mapping between this slice and the requested system
+ my @coords = $asm_mapper->map($normal_slice->seq_region_name(),
+ $normal_slice->start(),
+ $normal_slice->end(),
+ $normal_slice->strand(),
+ $slice_cs);
+
+ #construct a projection from the mapping results and return it
+
+ foreach my $coord (@coords) {
+ my $coord_start = $coord->start();
+ my $coord_end = $coord->end();
+ my $length = $coord_end - $coord_start + 1;
+
+ #skip gaps
+ if($coord->isa('Bio::EnsEMBL::Mapper::Coordinate')) {
+ #create slices for the mapped-to coord system
+ my $slice = $self->new
+ (-COORD_SYSTEM => $cs,
+ -START => $coord_start,
+ -END => $coord_end,
+ -STRAND => $coord->strand(),
+ -SEQ_REGION_NAME => $coord->id(),
+ -ADAPTOR => $self->adaptor());
+
+ my $current_end = $current_start + $length - 1;
+
+ push @projection, [$current_start, $current_end, $slice];
+ }
+
+ $current_start += $length;
+ }
+ }
+
+ return \@projection;
+}
+
+
+
+=head2 expand
+
+ Arg [1] : (optional) int $five_prime_expand
+ The number of basepairs to shift this slices five_prime
+ coordinate by. Positive values make the slice larger,
+ negative make the slice smaller.
+ coordinate left.
+ Default = 0.
+ Arg [2] : (optional) int $three_prime_expand
+ The number of basepairs to shift this slices three_prime
+ coordinate by. Positive values make the slice larger,
+ negative make the slice smaller.
+ Default = 0.
+ Example : my $expanded_slice = $slice->expand( 1000, 1000);
+ my $contracted_slice = $slice->expand(-1000,-1000);
+ my $shifted_right_slice = $slice->expand(-1000, 1000);
+ my $shifted_left_slice = $slice->expand( 1000,-1000);
+ Description: Returns a slice which is a resized copy of this slice. The
+ start and end are moved outwards from the center of the slice
+ if positive values are provided and moved inwards if negative
+ values are provided. This slice remains unchanged. A slice
+ may not be contracted below 1bp but may grow to be arbitrarily
+ large.
+ Returntype : Bio::EnsEMBL::Slice
+ Exceptions : warning if an attempt is made to contract the slice below 1bp
+ Caller : general
+
+=cut
+
+sub expand {
+ my $self = shift;
+ my $five_prime_shift = shift || 0;
+ my $three_prime_shift = shift || 0;
+
+ if($self->{'seq'}){
+ warning("You can't expand a slice which already has an attached ".
+ "sequence ");
+ return;
+ }
+
+ my $new_start;
+ my $new_end;
+
+ if($self->{'strand'} == 1) {
+ $new_start = $self->{'start'} - $five_prime_shift;
+ $new_end = $self->{'end'} + $three_prime_shift;
+ } else {
+ $new_end = $self->{'end'} + $five_prime_shift;
+ $new_start = $self->{'start'} - $three_prime_shift;
+ }
+
+ if($new_start > $new_end) {
+ throw('Slice start cannot be greater than slice end');
+ }
+
+ #fastest way to copy a slice is to do a shallow hash copy
+ my %new_slice = %$self;
+ $new_slice{'start'} = $new_start;
+ $new_slice{'end'} = $new_end;
+
+ return bless \%new_slice, ref($self);
+}
+
+
+
+=head2 get_seq_region_id
+
+ Arg [1] : none
+ Example : my $seq_region_id = $slice->get_seq_region_id();
+ Description: Gets the internal identifier of the seq_region that this slice
+ is on. Note that this function will not work correctly if this
+ slice does not have an attached adaptor. Also note that it may
+ be better to go through the SliceAdaptor::get_seq_region_id
+ method if you are working with multiple databases since is
+ possible to work with slices from databases with different
+ internal seq_region identifiers.
+ Returntype : int
+ Exceptions : warning if slice is not associated with a SliceAdaptor
+ Caller : assembly loading scripts, general
+
+=cut
+
+sub get_seq_region_id {
+ my ($self) = @_;
+
+ if($self->adaptor) {
+ return $self->adaptor->get_seq_region_id($self);
+ } else {
+ warning('Cannot retrieve seq_region_id without attached adaptor.');
+ return 0;
+ }
+}
+
+
+=head2 get_all_attribute_types
+
+ Arg [1] : none
+ Example : my @attribute_types = $slice->get_attribute_types();
+ Description: Gets a list of the types of attributes associated with the
+ seq_region this slice is on.
+ Returntype : list of strings
+ Exceptions : warning if slice does not have attached adaptor
+ Caller : general
+
+=cut
+
+sub get_attribute_types {
+ my $self = shift;
+
+ if(!$self->adaptor()) {
+ warning('Cannot get attributes without an adaptor.');
+ return ();
+ }
+ my %attrib_hash = %{$self->adaptor->get_seq_region_attribs($self)};
+ return keys %attrib_hash;
+}
+
+
+=head2 get_attribute
+
+ Arg [1] : string $attrib_type
+ The code of the attribute type to retrieve values for.
+ Example : ($htg_phase) = $slice->get_attribute('htg_phase');
+ @synonyms = $slice->get_attribute('synonyms');
+ Description: Gets a list of values for a given attribute of this
+ slice''s seq_region.
+ Returntype : list of strings
+ Exceptions : warning is slice does not have attached adaptor
+ throw if argument is not provided
+ Caller : general
+
+=cut
+
+sub get_attribute {
+ my $self = shift;
+ my $attrib_code = shift;
+
+ throw('Attrib code argument is required.') if(!$attrib_code);
+
+ if(!$self->adaptor()) {
+ warning('Cannot get attributes without an adaptor.');
+ return ();
+ }
+
+ my %attribs = %{$self->adaptor->get_seq_region_attribs($self)};
+
+ return @{$attribs{$attrib_code} || []};
+}
+
+
=head2 get_all_PredictionTranscripts
Arg [1] : (optional) string $logic_name
@@ -510,6 +847,9 @@ sub get_all_PredictionTranscripts {
to obtain.
Arg [2] : (optional) float $score
The mimimum score of the features to retrieve
+ Arg [3] : (optional) string $dbtype
+ The name of an attached database to retrieve the features from
+ instead. e.g. 'est'.
Example : @dna_dna_align_feats = @{$slice->get_all_DnaAlignFeatures};
Description: Retrieves the DnaDnaAlignFeatures which overlap this slice with
logic name $logic_name and with score above $score. If
@@ -523,17 +863,24 @@ sub get_all_PredictionTranscripts {
=cut
sub get_all_DnaAlignFeatures {
- my ($self, $logic_name, $score, $dbtype) = @_;
+ my ($self, $logic_name, $score, $dbtype) = @_;
- my $db = ($dbtype) ? $self->adaptor->db->get_db_adaptor($dbtype) : $self->adaptor->db;
+ my $db;
- if(!$db) {
- $self->warn("Don't have db $dbtype returning empty list\n");
- return [];
- }
+ if($dbtype) {
+ $db = $self->adaptor->db->get_db_adaptor($dbtype);
+ if(!$db) {
+ warning("Don't have db $dbtype returning empty list\n");
+ return [];
+ }
+ } else {
+ $db = $self->adaptor->db;
+ }
- return $db->get_DnaAlignFeatureAdaptor()->fetch_all_by_Slice_and_score($self, $score, $logic_name);
-}
+ my $dafa = $db->get_DnaAlignFeatureAdaptor();
+
+ return $dafa->fetch_all_by_Slice_and_score($self,$score, $logic_name);
+}
@@ -544,6 +891,9 @@ sub get_all_DnaAlignFeatures {
to obtain.
Arg [2] : (optional) float $score
The mimimum score of the features to retrieve
+ Arg [3] : (optional) string $dbtype
+ The name of an attached datbase to retrieve features from
+ instead.
Example : @dna_pep_align_feats = @{$slice->get_all_ProteinAlignFeatures};
Description: Retrieves the DnaPepAlignFeatures which overlap this slice with
logic name $logic_name and with score above $score. If
@@ -559,14 +909,20 @@ sub get_all_DnaAlignFeatures {
sub get_all_ProteinAlignFeatures {
my ($self, $logic_name, $score, $dbtype) = @_;
- my $db = ($dbtype) ? $self->adaptor->db->get_db_adaptor($dbtype) : $self->adaptor->db;
+ my $db;
- if(!$db) {
- $self->warn("Don't have db $dbtype returning empty list\n");
- return [];
+ if($dbtype) {
+ $db = $self->adaptor->db->get_db_adaptor($dbtype);
+ if(!$db) {
+ warning("Don't have db $dbtype returning empty list\n");
+ return [];
+ }
+ } else {
+ $db = $self->adaptor->db;
}
- return $db->get_ProteinAlignFeatureAdaptor()->fetch_all_by_Slice_and_score($self, $score, $logic_name);
+ my $pafa = $db->get_ProteinAlignFeatureAdaptor();
+ return $pafa->fetch_all_by_Slice_and_score($self, $score, $logic_name);
}
@@ -592,10 +948,13 @@ sub get_all_ProteinAlignFeatures {
=cut
sub get_all_SimilarityFeatures {
- my $self = shift;
+ my ($self, $logic_name, $score) = @_;
+
+ my @out = ();
+
+ push @out, @{$self->get_all_ProteinAlignFeatures($logic_name, $score) };
+ push @out, @{$self->get_all_DnaAlignFeatures($logic_name, $score) };
- my @out = (@{$self->get_all_ProteinAlignFeatures( @_ ) },
- @{$self->get_all_DnaAlignFeatures( @_ ) });
return \@out;
}
@@ -655,6 +1014,7 @@ sub get_all_RepeatFeatures {
}
+
=head2 get_all_SNPs
Args : none
@@ -710,12 +1070,7 @@ sub get_all_Genes{
-
-
-
-
-
-=head2 get_all_Genes_by_type
+=head2 get_all_Genes_by_type
Arg [1] : string $type
@@ -723,17 +1078,17 @@ sub get_all_Genes{
Arg [3] : (optional) boolean $empty_flag
Example : @genes = @{$slice->get_all_Genes_by_type($type,
'ensembl')};
- Description: Retrieves genes that overlap this slice of type $type.
- This is primarily used by the genebuilding code when several
+ Description: Retrieves genes that overlap this slice of type $type.
+ This is primarily used by the genebuilding code when several
types of genes are used.
-
+
The logic name is the analysis of the genes that are retrieved.
If not provided all genes will be retrieved instead.
-
- The empty flag indicates light weight genes that only have a
- start, end and strand should be used (only works if lite db is
- available). If the lite database has
- been attached to the core database this method will use the
+
+ The empty flag indicates light weight genes that only have a
+ start, end and strand should be used (only works if lite db is
+ available). If the lite database has
+ been attached to the core database this method will use the
lite database (and genes will not be as full featured).
Returntype : listref of Bio::EnsEMBL::Genes
Exceptions : none
@@ -743,183 +1098,81 @@ sub get_all_Genes{
sub get_all_Genes_by_type{
my ($self, $type, $logic_name, $empty_flag) = @_;
-
+
my @out = grep { $_->type eq $type } @{ $self->get_all_Genes($logic_name,
$empty_flag)};
-
- return \@out;
-}
-
-
-
-
-=head2 chr_name
-
- Arg [1] : (optional) string $value
- Example : $chr_name = $slice->chr_name;
- Description: Getter/Setter for the name of the chromosome that this slice
- is on. This is generally set by the SliceAdaptor and should
- probably not be set outside of that context.
- Returntype : string
- Exceptions : none
- Caller : SliceAdaptor
-
-=cut
-
-sub chr_name{
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'chr_name'} = $value;
- }
- return $self->{'chr_name'};
-
-}
-
-
-
-=head2 chr_start
-
- Arg [1] : int $value
- Example : $chr_start = $slice->chr_start;
- Description: Getter/Setter for the start base of this slice on the
- chromosome. This is generally set by the SliceAdaptor and
- probably shouldnt be set outside of that context.
- chr_start is always less than or equal to chr_end
- Returntype : int
- Exceptions : none
- Caller : SliceAdaptor, general
-
-=cut
-sub chr_start{
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'chr_start'} = $value;
- }
- return $self->{'chr_start'};
-}
-
-
-
-=head2 chr_end
-
- Arg [1] : int $value
- Example : $chr_end = $slice->chr_end;
- Description: Getter/Setter for the end base of this slice on the
- chromosome. This is generally set by the SliceAdaptor and
- probably shouldnt be set outside of that context.
- chr_end is always greater than or equal to chr_start
- Returntype : int
- Exceptions : none
- Caller : SliceAdaptor, general
-
-=cut
-
-sub chr_end{
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'chr_end'} = $value;
- }
- return $self->{'chr_end'};
+ return \@out;
}
+=head2 get_all_Transcripts
-=head2 strand
-
- Arg [1] : int $value
- Example : $strand = $slice->strand;
- Description: Getter/Setter for the strand of the chromosome this slice is on.
- This should not be set manually. A much better way to obtain
- a slice on the opposite strand is to call the invert method.
- Returntype : int (either 1 or -1)
+ Arg [1] : none
+ Example : @transcripts = @{$slice->get_all_Transcripts};
+ Description: Gets all transcripts which overlap this slice. If you want to
+ specify a particular analysis or type, then you are better off
+ using get_all_Genes or get_all_Genes_by_type and iterating
+ through the transcripts of each gene.
+ Returntype : reference to a list of Bio::EnsEMBL::Transcripts
Exceptions : none
- Caller : invert, SliceAdaptor, general
+ Caller : general
=cut
-sub strand{
- my ($self,$value) = @_;
-
- if( defined $value) {
- $self->{'strand'} = $value;
- }
- return $self->{'strand'};
-
+sub get_all_Transcripts {
+ my $self = shift;
+ return $self->adaptor->db->get_TranscriptAdaptor->fetch_all_by_Slice($self);
}
+=head2 get_all_Exons
-=head2 assembly_type
-
- Arg [1] : string $value
- Example : $assembly_mapper_adaptor->fetch_by_type($slice->assembly_type);
- Description: Gets/Sets the assembly type that this slice is constructed
- from. This is generally set by the slice adaptor and probably
- shouldnt be set outside of this context.
- Returntype : string
+ Arg [1] : none
+ Example : @exons = @{$slice->get_all_Exons};
+ Description: Gets all exons which overlap this slice. Note that these exons
+ will not be associated with any transcripts, so this may not
+ be terribly useful.
+ Returntype : reference to a list of Bio::EnsEMBL::Exons
Exceptions : none
Caller : general
=cut
-sub assembly_type {
- my ($self,$value) = @_;
- if( defined $value) {
- $self->{'assembly_type'} = $value;
- }
- return $self->{'assembly_type'};
-
+sub get_all_Exons {
+ my $self = shift;
+ return $self->adaptor->db->get_ExonAdaptor->fetch_all_by_Slice($self);
}
-=head2 assembly_name
+=head2 get_all_QtlFeatures
- Arg [1] : string $value
- Example : $slice->assembly_name();
- Description: Gets/Sets the assembly name that this slice is constructed
- from. This is generally set by the slice adaptor and probably
- shouldnt be set outside of this context.
- Returntype : string
+ Args : none
+ Example : none
+ Description: returns overlapping QtlFeatures
+ Returntype : listref Bio::EnsEMBL::Map::QtlFeature
Exceptions : none
Caller : general
=cut
-sub assembly_name {
- my ($self,$value) = @_;
- if( defined $value) {
- $self->adaptor->db->assembly_name($value);
- }
- return $self->adaptor->db->assembly_name();
-
-}
-
-=head2 assembly_version
-
- Arg [1] : string $value
- Example : $slice->assembly_version();
- Description: Gets/Sets the assembly version that this slice is constructed
- from. This is generally set by the slice adaptor and probably
- shouldnt be set outside of this context.
- Returntype : string
- Exceptions : none
- Caller : general
-
-=cut
+sub get_all_QtlFeatures {
+ my $self = shift;
-sub assembly_version {
- my ($self,$value) = @_;
- if( defined $value) {
- $self->adaptor->db->assembly_version($value);
- }
- return $self->adaptor->db->assembly_version();
+ my $qfAdaptor;
+ if( $self->adaptor()) {
+ $qfAdaptor = $self->adaptor()->db()->get_QtlFeatureAdaptor();
+ } else {
+ return [];
+ }
+ return $qfAdaptor->fetch_all_by_Slice_constraint( $self );
}
+
=head2 get_all_KaryotypeBands
Arg [1] : none
@@ -933,171 +1186,94 @@ sub assembly_version {
sub get_all_KaryotypeBands {
my ($self) = @_;
-
+
my $kadp = $self->adaptor->db->get_KaryotypeBandAdaptor();
return $kadp->fetch_all_by_Slice($self);
}
-=head2 get_Chromosome
-
- Arg [1] : none
- Example : $chromosome = $slice->get_Chromosome;
- Description: Retrieves the chromosome object which this slice is on
- Returntype : Bio::EnsEMBL::Chromosome
- Exceptions : none
- Caller : general
-
-=cut
-
-sub get_Chromosome {
- my $self = shift @_;
-
- my $ca = $self->adaptor->db->get_ChromosomeAdaptor();
-
- return $ca->fetch_by_chr_name($self->chr_name());
-}
-
-
=head2 get_repeatmasked_seq
- Arg [1] : listref of strings $logic_names (optional, default is '')
- Arg [2] : int $default_masking_type (optional, default is 0)
- 0 hard mask, repeats are replaced by Ns
- 1 soft mask, repeats are transformed in lower case
- Arg [3] : hash reference $not_default_masking_cases (optional, default is {})
- The values are 0 or 1 with same definition as in Arg [2]
- The keys of the hash should be of 2 forms
- "repeat_class_" . $repeat_consensus->repeat_class,
- e.g. "repeat_class_SINE/MIR"
- "repeat_name_" . $repeat_consensus->name
- e.g. "repeat_name_MIR"
- depending on which based you want to apply the not default masking,
- on the repeat_class or repeat_name. Both can be specified in the same hash
- the same time, but in that case, repeat_name setting has priority over
- repeat_class. For example, you may have hard maksing as default, and
- you may want soft masking of all repeat_name SINE/MIR,
- but repeat_name AluSp (which are also from repeat_name SINE/MIR)
- Example : $slice->get_repeatmasked_seq
- or $slice->get_repeatmasked_seq(['RepeatMask'],1)
- Description: Returns Bio::PrimarySeq containing the masked (repeat replaced
- by N)
- or soft-masked (when Arg[2]=1, repeat in lower case while non
- repeat in upper case) sequence corresponding to the Slice
- object.
+ Arg [1] : listref of strings $logic_names (optional)
+ Arg [2] : int $soft_masking_enable (optional)
+ Example : $rm_slice = $slice->get_repeatmasked_seq()
+ $softrm_slice->get_repeatmasked_seq(['RepeatMask'],1)
+ Description: Returns Bio::EnsEMBL::Slice that can be used to create repeat
+ masked sequence instead of the regular sequence.
+ Sequence returned by this new slice will have repeat regions
+ hardmasked by default (sequence replaced by N) or
+ or soft-masked when arg[2] = 1 (sequence in lowercase)
Will only work with database connection to get repeat features.
- Returntype : Bio::PrimarySeq
+ Returntype : Bio::EnsEMBL::RepeatMaskedSlice
Exceptions : none
Caller : general
=cut
sub get_repeatmasked_seq {
- my ($self,$logic_names,$default_masking_type, $not_default_masking_cases) = @_;
-
- unless($logic_names && @$logic_names) {
- $logic_names = [ '' ];
- }
-
- unless (defined $default_masking_type) {
- $default_masking_type = 0;
- # 0 hard mask, repeats are replaced by Ns
- # 1 soft mask, repeats are transformed in lower case
- }
-
- unless (defined $not_default_masking_cases) {
- $not_default_masking_cases = {};
- }
-
- my $repeats = [];
-
- foreach my $l (@$logic_names) {
- push @{$repeats}, @{$self->get_all_RepeatFeatures($l)};
- }
-
- my $dna = $self->seq();
- my $masked_dna = $self->_mask_features($dna,$repeats,$default_masking_type,$not_default_masking_cases);
- my $masked_seq = Bio::PrimarySeq->new('-seq' => $masked_dna,
- '-display_id' => $self->id,
- '-primary_id' => $self->id,
- '-moltype' => 'dna'
- );
- return $masked_seq;
+ my ($self,$logic_names,$soft_mask) = @_;
+
+ #creat a slice that
+ return Bio::EnsEMBL::RepeatMaskedSlice->new
+ (-START => $self->{'start'},
+ -END => $self->{'end'},
+ -STRAND => $self->{'strand'},
+ -ADAPTOR => $self->{'adaptor'},
+ -SEQ => $self->{'seq'},
+ -SEQ_REGION_NAME => $self->{'seq_region_name'},
+ -COORD_SYSTEM => $self->{'coord_system'},
+ -REPEAT_MASK => $logic_names,
+ -SOFT_MASK => $soft_mask);
}
=head2 _mask_features
- Arg [1] : string $dna_string
+ Arg [1] : reference to a string $dnaref
Arg [2] : array_ref $repeats
reference to a list Bio::EnsEMBL::RepeatFeature
give the list of coordinates to replace with N or with
lower case
- Arg [3] : int $default_masking_type (optional)
- 0 hard mask, repeats are replaced by Ns (default)
- 1 soft mask, repeats are transformed in lower case
- Arg [4] : hash reference $not_default_masking_cases (optional)
- The values are 0 or 1 with same definition as in Arg [3]
- The keys of the hash should be of 2 forms
- "repeat_class_" . $repeat_consensus->repeat_class,
- e.g. "repeat_class_SINE/MIR"
- "repeat_name_" . $repeat_consensus->name
- e.g. "repeat_name_MIR"
- depending on which based you want to apply the not default masking,
- on the repeat_class or repeat_name. Both can be specified in the same hash
- the same time, but in that case, repeat_name setting has priority over
- repeat_class. For example, you may have hard maksing as default, and
- you may want soft masking of all repeat_name SINE/MIR,
- but repeat_name AluSp (which are also from repeat_name SINE/MIR)
+ Arg [3] : int $soft_masking_enable (optional)
Example : none
Description: replaces string positions described in the RepeatFeatures
with Ns (default setting), or with the lower case equivalent
- (soft masking)
- Returntype : string
+ (soft masking). The reference to a dna string which is passed
+ is changed in place.
+ Returntype : none
Exceptions : none
- Caller : get_repeatmasked_seq
+ Caller : seq
=cut
sub _mask_features {
- my ($self,$dnastr,$repeats,$default_masking_type,$not_default_masking_cases) = @_;
-
- # explicit CORE::length call, to avoid any confusion with the Slice
+ my ($self,$dnaref,$repeats,$soft_mask) = @_;
+
+ # explicit CORE::length call, to avoid any confusion with the Slice
# length method
- my $dnalen = CORE::length($dnastr);
-
- unless (defined $default_masking_type) {
- $default_masking_type = 0;
- # 0 hard mask, repeats are replaced by Ns
- # 1 soft mask, repeats are transformed in lower case
- }
- unless (defined $not_default_masking_cases) {
- $not_default_masking_cases = {};
- }
+ my $dnalen = CORE::length($$dnaref);
REP:foreach my $f (@{$repeats}) {
my $start = $f->start;
my $end = $f->end;
my $length = ($end - $start) + 1;
-
+
# check if we get repeat completely outside of expected slice range
if ($end < 1 || $start > $dnalen) {
- $self->warn("Repeat completely outside slice coordinates! " .
- "That should not happen! repeat_start $start or repeat_end $end not" .
- "within [1-$dnalen] slice range coordinates\n");
+ warning("Unexpected: Repeat completely outside slice coordinates.");
next REP;
}
-
+
# repeat partly outside slice range, so correct
# the repeat start and length to the slice size if needed
if ($start < 1) {
$start = 1;
$length = ($end - $start) + 1;
}
-
+
# repeat partly outside slice range, so correct
# the repeat end and length to the slice size if needed
if ($end > $dnalen) {
@@ -1106,34 +1282,17 @@ sub _mask_features {
}
$start--;
-
+
my $padstr;
- my $repeat_consensus = $f->repeat_consensus;
- my $masking_type = $default_masking_type;
-
- if (defined $not_default_masking_cases->{"repeat_class_" . $repeat_consensus->repeat_class}) {
- $masking_type = $not_default_masking_cases->{"repeat_class_" . $repeat_consensus->repeat_class};
- }
- if (defined $not_default_masking_cases->{"repeat_name_" . $repeat_consensus->name}) {
- $masking_type = $not_default_masking_cases->{"repeat_name_" . $repeat_consensus->name};
- }
-
- if ($masking_type) {
- print STDERR "LowerCase Masking: ",$repeat_consensus->repeat_class," ",$repeat_consensus->name,"\n";
- $padstr = lc substr ($dnastr,$start,$length);
+ if ($soft_mask) {
+ $padstr = lc substr ($$dnaref,$start,$length);
} else {
- print STDERR "Hard Masking: ",$repeat_consensus->repeat_class," ",$repeat_consensus->name,"\n";
$padstr = 'N' x $length;
}
- substr ($dnastr,$start,$length) = $padstr;
-
- }
- unless (scalar @{$repeats}) {
- print STDERR "No Repeat Features\n";
+ substr ($$dnaref,$start,$length) = $padstr;
}
- return $dnastr;
-}
+}
=head2 get_all_SearchFeatures
@@ -1154,7 +1313,7 @@ sub get_all_SearchFeatures {
local $_;
unless($ticket) {
- $self->throw("ticket argument is required");
+ throw("ticket argument is required");
}
my $sfa = $self->adaptor()->db()->get_db_adaptor('blast');
@@ -1162,7 +1321,7 @@ sub get_all_SearchFeatures {
my $offset = $self->chr_start-1;
my $features = $sfa ? $sfa->get_all_SearchFeatures($ticket, $self->chr_name, $self->chr_start, $self->chr_end) : [];
- #warn join(', ', @$features );
+
foreach( @$features ) {
$_->start( $_->start-$offset );
$_->end( $_->end-$offset );
@@ -1171,122 +1330,41 @@ sub get_all_SearchFeatures {
}
-=head2 get_all_MapFrags
- Arg [1] : string $mapset
- Example : $slice->get_all_MapFrags('cloneset');
- Description: Retreives all mapfrags of mapset $mapset that overlap this slice
- Returntype : listref of Bio::EnsEMBL::MapFrags
+=head2 get_all_MiscFeatures
+
+ Arg [1] : string $set (optional)
+ Example : $slice->get_all_MiscFeatures('cloneset');
+ Description: Retreives all misc features which overlap this slice. If
+ a set code is provided only features which are members of
+ the requested set are returned.
+ Returntype : listref of Bio::EnsEMBL::MiscFeatures
Exceptions : none
Caller : general
=cut
-sub get_all_MapFrags {
- my $self = shift;
- my $mapset = shift;
-
- unless($mapset) {
- $self->throw("mapset argument is required");
- }
-
- my $mfa = $self->adaptor()->db()->get_MapFragAdaptor();
-
- return $mfa->fetch_all_by_mapset_chr_start_end($mapset,
- $self->chr_name,
- $self->chr_start,
- $self->chr_end);
-}
-
-
-
-sub has_MapSet {
- my( $self, $mapset_name ) = @_;
-
- my $mfa = $self->adaptor()->db()->get_MapFragAdaptor();
-
- return $mfa->has_mapset($mapset_name);
-}
-
-
+sub get_all_MiscFeatures {
+ my $self = shift;
+ my $misc_set = shift;
-=head2 get_tiling_path
+ my $adaptor = $self->adaptor();
- Arg [1] : none
- Example : @tiles = @{$slice->get_tiling_path()};
- Description: Retrieve a listref of Bio::EnsEMBL::Tile objects representing
- the tiling path used to construct the contiguous slice sequence.
- Returntype : list reference of Bio::EnsEMBL::Tile objects
- Exceptions : none
- Caller : general
+ if(!$adaptor) {
+ warning('Cannot retrieve features without attached adaptor.');
+ return [];
+ }
-=cut
+ my $mfa = $adaptor->db->get_MiscFeatureAdaptor();
-sub get_tiling_path {
- my ($self) = @_;
-
- my $mapper = $self->adaptor()->db->get_AssemblyMapperAdaptor()->
- fetch_by_type($self->assembly_type());
-
- # Get the ids of the raw_contigs in this region specified in chrmsml coords
-
- my @mapped = $mapper->map_coordinates_to_rawcontig
- (
- $self->chr_name(),
- $self->chr_start(),
- $self->chr_end(),
- $self->strand()
- );
-
- # Extract the IDS of the Coordinates, ommitting Gaps
- my @raw_contig_ids = ();
- foreach my $map_item (@mapped) {
- if($map_item->isa("Bio::EnsEMBL::Mapper::Coordinate" )) {
- push @raw_contig_ids, $map_item->id();
- }
+ if($misc_set) {
+ return $mfa->fetch_all_by_Slice_and_set_code($self,$misc_set);
}
- #Fetch filled raw contigs (non lazy-loaded) containing filled clone objects
- my $rca = $self->adaptor->db->get_RawContigAdaptor();
- my $raw_contigs = $rca->fetch_filled_by_dbIDs(@raw_contig_ids);
+ return $mfa->fetch_all_by_Slice($self);
+}
- my @tiling_path = ();
- my $current_start = 1;
- my($length, $slice_start, $slice_end,
- $contig, $contig_start, $contig_end, $contig_ori);
-
- foreach my $coord ( @mapped ) {
- $contig_start = $coord->start();
- $contig_end = $coord->end();
- $length = $contig_end - $contig_start + 1;
-
- if ( $coord->isa("Bio::EnsEMBL::Mapper::Coordinate" ) ) {
- # create a tile for each coordinate
- $contig_ori = $coord->strand();
- $slice_start = $current_start;
- $slice_end = $current_start + $length - 1;
- $contig = $raw_contigs->{ $coord->id() };
-
- push @tiling_path, Bio::EnsEMBL::Tile->new_fast($self,
- $slice_start,
- $slice_end,
- $contig,
- $contig_start,
- $contig_end,
- $contig_ori);
-
-
-
- $current_start += $length;
- } else {
- # this is a gap, just add the length and discard it
- $current_start += $length;
- }
- }
- return \@tiling_path;
-}
-
=head2 get_all_MarkerFeatures
@@ -1324,9 +1402,8 @@ sub get_all_MarkerFeatures {
Arg [1] : string $qy_species
The name of the species to retrieve similarity features from
- Arg [2] : string $qy_assembly (can be undef)
+ Arg [2] : string $qy_assembly
The name of the assembly to retrieve similarity features from
- if undef assembly_default will be queried from compara db.
Arg [3] : string $type
The type of the alignment to retrieve similarity features from
Example : $fs = $slc->get_all_compara_DnaAlignFeatures('Mus musculus',
@@ -1347,18 +1424,14 @@ sub get_all_MarkerFeatures {
sub get_all_compara_DnaAlignFeatures {
my ($self, $qy_species, $qy_assembly, $alignment_type) = @_;
- unless($qy_species && $alignment_type) {
- $self->throw("Query species and alignmemt type arguments are required");
- }
-
- unless (defined $qy_assembly) {
- warn "qy_assembly undef, will query the default one\n";
+ unless($qy_species && $qy_assembly && $alignment_type) {
+ throw("Query species and assembly and alignmemt type arguments are required");
}
my $compara_db = $self->adaptor->db->get_db_adaptor('compara');
unless($compara_db) {
- $self->warn("Compara database must be attached to core database to " .
+ warning("Compara database must be attached to core database to " .
"retrieve compara information");
return [];
}
@@ -1371,13 +1444,13 @@ sub get_all_compara_Syntenies {
my ($self, $qy_species ) = @_;
unless($qy_species) {
- $self->throw("Query species and assembly arguments are required");
+ throw("Query species and assembly arguments are required");
}
my $compara_db = $self->adaptor->db->get_db_adaptor('compara');
unless($compara_db) {
- $self->warn("Compara database must be attached to core database to " .
+ warning("Compara database must be attached to core database to " .
"retrieve compara information");
return [];
}
@@ -1408,7 +1481,7 @@ sub get_all_Haplotypes {
my $haplo_db = $self->adaptor->db->get_db_adaptor('haplotype');
unless($haplo_db) {
- $self->warn("Haplotype database must be attached to core database to " .
+ warning("Haplotype database must be attached to core database to " .
"retrieve haplotype information" );
return [];
}
@@ -1469,7 +1542,7 @@ sub get_all_ExternalFeatures {
my $features = [];
- my $xfa_hash = $self->adaptor->db->get_ExternalFeatureAdaptors();
+ my $xfa_hash = $self->adaptor->db->get_ExternalFeatureAdaptors;
my @xf_adaptors = ();
if($track_name) {
@@ -1489,217 +1562,343 @@ sub get_all_ExternalFeatures {
}
-=head2 get_all_ExternalLiteFeatures
- Arg [1] : (optional) string $track_name
- If specified only features from ExternalFeatureAdaptors with
- the track name $track_name are retrieved.
- If not set, all features from every ExternalFeatureAdaptor are
- retrieved.
- Example : @x_features = @{$slice->get_all_ExternalFeatures}
- Description: Retrieves features on this slice from external feature adaptors
- Returntype : listref of light Bio::SeqFeatureI implementing objects in slice
- coordinates
+
+
+# GENERIC FEATURES (See DBAdaptor.pm)
+
+=head2 get_generic_features
+
+ Arg [1] : (optional) List of names of generic feature types to return.
+ If no feature names are given, all generic features are
+ returned.
+ Example : my %features = %{$slice->get_generic_features()};
+ Description: Gets generic features via the generic feature adaptors that
+ have been added via DBAdaptor->add_GenericFeatureAdaptor (if
+ any)
+ Returntype : Hash of named features.
Exceptions : none
- Caller : general
+ Caller : none
=cut
-sub get_all_ExternalLiteFeatures {
- my ($self, $track_name) = @_;
+sub get_generic_features() {
- my $features = [];
- my $want_light = 1;
-
- my $xfa_hash = $self->adaptor->db->get_ExternalFeatureAdaptors();
- my @xf_adaptors = ();
+ my ($self, @names) = @_;
- if($track_name) {
- #use a specific adaptor
- push @xf_adaptors, $xfa_hash->{$track_name};
- } else {
- #use all of the adaptors
- push @xf_adaptors, values %$xfa_hash;
- }
+ my $db = $self->adaptor()->db();
+ my %features = (); # this will hold the results
- foreach my $xfa (@xf_adaptors) {
- push @$features, @{$xfa->fetch_all_by_Slice($self, $want_light)};
- }
+ # get the adaptors for each feature
+ my %adaptors = %{$db->get_GenericFeatureAdaptors(@names)};
+
+ foreach my $adaptor_name (keys(%adaptors)) {
+
+ my $adaptor_obj = $adaptors{$adaptor_name};
+ # get the features and add them to the hash
+ my $features_ref = $adaptor_obj->fetch_all_by_Slice($self);
+ # add each feature to the hash to be returned
+ foreach my $feature (@$features_ref) {
+ $features{$adaptor_name} = $feature;
+ }
+ }
+
+ return \%features;
- return $features;
}
-=head2 Methods included only for BioPerl compliance
-=cut
+# sub DEPRECATED METHODS #
###############################################################################
-=head2 display_id
+=head1 DEPRECATED METHODS
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance.
- Returntype : string
- Exceptions : none
- Caller : none
+=cut
+
+
+
+=head2 get_all_supercontig_Slices
+
+ Description: DEPRECATED use get_tiling_path("NTcontig") instead
=cut
-sub display_id {
- my ($self, $value) = @_;
+sub get_all_supercontig_Slices {
+ my $self = shift;
- if(!$self->{'_display_id'}){
- $self->{'_display_id'} = undef;
- }
- if( defined $value ) {
- $self->{'_display_id'} = $value;
- }
- my $id = $self->{'_display_id'};
- if(!$id){
- $id = $self->id;
- }
+ deprecate("Use get_tiling_path('NTcontig') instead");
+
+ my $result = [];
- return $id;
+ if( $self->adaptor() ) {
+ my $superctg_names =
+ $self->adaptor()->list_overlapping_supercontigs( $self );
+
+ for my $name ( @$superctg_names ) {
+ my $slice;
+ $slice = $self->adaptor()->fetch_by_supercontig_name( $name );
+ $slice->name( $name );
+ push( @$result, $slice );
+ }
+ } else {
+ warning( "Slice needs to be attached to a database to get supercontigs" );
+ }
+
+ return $result;
}
+
+=head2 get_all_Genes_by_source
+
+ Description: DEPRECATED use get_all_Genes instead
+
+=cut
+
+sub get_all_Genes_by_source {
+ deprecate("Use get_all_Genes() instead");
+ return get_all_Genes(@_);
+}
+
+
+=head2 id
+
+ Description: DEPRECATED use name() instead
+
+=cut
+
+sub id {
+ deprecate("Use name() instead");
+ name(@_);
+}
+
+
+
+=head2 display_id
+
+ Description: DEPRECATED use name() instead
+
+=cut
+
+
+sub display_id {
+ deprecate("Use name() instead");
+ name(@_);
+}
+
+
=head2 desc
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance
- Returntype : none
- Exceptions : none
- Caller : none
+ Description: DEPRECATED use name() instead
=cut
sub desc{
- my $self = shift;
- return "Slice, no description";
+ deprecate("Use name() instead");
}
=head2 moltype
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance
- Returntype : none
- Exceptions : none
- Caller : none
+ Description: DEPRECATED do not use
=cut
sub moltype {
- my $self = shift;
+ deprecate("Do not use this method");
return 'dna';
}
=head2 alphabet
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance
- Returntype : none
- Exceptions : none
- Caller : none
+ Description: DEPRECATED do not use
=cut
+
sub alphabet {
- my $self = shift;
+ deprecate("Do not use this method");
return 'dna';
}
=head2 accession_number
- Arg [1] : none
- Example : none
- Description: Only for BioPerl compliance
- Returntype : none
- Exceptions : none
- Caller : none
+ Description: DEPRECATED use name() instead
=cut
sub accession_number {
- my $self = shift;
- return $self->dbID();
+ deprecate("Use name() instead");
+ name(@_);
}
+=head2 get_Chromosome
+ Description: DEPRECATED use this instead:
+ $slice_adp->fetch_by_region('chromosome',
+ $slice->seq_region_name)
-=head2 sub DEPRECATED methods
=cut
-###############################################################################
+sub get_Chromosome {
+ my $self = shift @_;
-# GENERIC FEATURES (See DBAdaptor.pm)
+ deprecate("Use SliceAdaptor::fetch_by_region('chromosome'," .
+ '$slice->seq_region_name) instead');
-=head2 get_generic_features
+ my $chr = $self->adaptor->fetch_by_region('toplevel',
+ $self->seq_region_name());
- Arg [1] : (optional) List of names of generic feature types to return. If no feature
- names are given, all generic features are returned.
- Example : my %features = $slice->get_generic_features()
- Description: Gets generic features via the generic feature adaptors that have been added
- via DBAdaptor->add_GenericFeatureAdaptor (if any)
- Returntype : Hash of named features.
+ return bless($chr, 'Bio::EnsEMBL::Chromosome');
+}
+
+
+
+=head2 chr_name
+
+ Description: DEPRECATED use seq_region_name() instead
+
+=cut
+
+sub chr_name{
+ deprecate("Use seq_region_name() instead");
+ seq_region_name(@_);
+}
+
+
+
+=head2 chr_start
+
+ Description: DEPRECATED use start() instead
+
+=cut
+
+sub chr_start{
+ deprecate('Use start() instead');
+ start(@_);
+}
+
+
+
+=head2 chr_end
+
+ Description: DEPRECATED use end() instead
+ Returntype : int
Exceptions : none
- Caller : none
+ Caller : SliceAdaptor, general
=cut
-sub get_generic_features() {
+sub chr_end{
+ deprecate('Use end() instead');
+ end(@_);
+}
- my ($self, @names) = @_;
- my $db = $self->adaptor()->db();
+=head2 assembly_type
- my %features = (); # this will hold the results
+ Description: DEPRECATED use version instead
- # get the adaptors for each feature
- my $adaptors = $db->get_GenericFeatureAdaptors(@names);
+=cut
- foreach my $adaptor_name (keys(%$adaptors)) {
-
- my $adaptor_obj = $adaptors->{$adaptor_name};
- # get the features and add them to the hash
- my $features_ref = $adaptor_obj->fetch_all_by_Slice($self);
- # add each feature to the hash to be returned
- foreach my $feature (@$features_ref) {
- $features{$adaptor_name} = $feature;
- }
- }
+sub assembly_type{
+ my $self = shift;
+ deprecate('Use $slice->coord_system()->version() instead.');
+ return $self->coord_system->version();
+}
- return \%features;
+=head2 get_tiling_path
+
+ Description: DEPRECATED use project instead
+
+=cut
+
+sub get_tiling_path {
+ my $self = shift;
+
+ deprecate('Use $slice->project("seqlevel") instead.');
+
+ my $csa = $self->adaptor()->db()->get_CoordSystemAdaptor();
+
+ #assume that they want the tiling path to the sequence coord system
+ #this might not work well if this isn't a chromosomal slice
+ my $projection = $self->project('seqlevel');
+
+ my @tiling_path;
+
+ foreach my $segment (@$projection) {
+
+ my ($slice_start, $slice_end, $contig) = @$segment;
+ my $contig_ori = $contig->strand();
+ my $contig_start = $contig->start();
+ my $contig_end = $contig->end();
+
+ #the old get_tiling_path always gave back entire contigs in the forward
+ #strand
+ $contig = $contig->adaptor->fetch_by_region('seqlevel',
+ $contig->seq_region_name());
+
+ #bless contigs into contigs
+ $contig = bless($contig, 'Bio::EnsEMBL::RawContig');
+
+
+ push @tiling_path, Bio::EnsEMBL::Tile->new_fast($self,
+ $slice_start,
+ $slice_end,
+ $contig,
+ $contig_start,
+ $contig_end,
+ $contig_ori);
+ }
+
+ return \@tiling_path;
}
+=head2 dbID
-# sub DEPRECATED METHODS #
-###############################################################################
+ Description: DEPRECATED use SliceAdaptor::get_seq_region_id instead
+=cut
-=head2 get_all_Genes_by_source
+sub dbID {
+ my $self = shift;
+ deprecate('Use SliceAdaptor::get_seq_region_id instead.');
+ if(!$self->adaptor) {
+ warning('Cannot retrieve seq_region_id without attached adaptor.');
+ return 0;
+ }
+ return $self->adaptor->get_seq_region_id($self);
+}
- Arg [1] : none
- Example : none
- Description: DEPRECATED use get_all_Genes instead
- Returntype : none
- Exceptions : none
- Caller : none
+
+=head2 get_all_MapFrags
+
+ Description: DEPRECATED use get_all_MiscFeatures instead
=cut
-sub get_all_Genes_by_source {
- my ($self, @args) = @_;
+sub get_all_MapFrags {
+ my $self = shift;
+ deprecate('Use get_all_MiscFeatures instead');
+ return $self->get_all_MiscFeatures(@_);
+}
+
+=head2 has_MapSet
- $self->warn("call to deprecated method get_all_Genes_by_source. " .
- "Use get_all_Genes instead\n " . join(':', caller));
+ Description: Not sure how this function is used, but it probably
+ is not necessary.
- return $self->get_all_Genes;
+=cut
+
+sub has_MapSet {
+ my( $self, $mapset_name ) = @_;
+ deprecate('Not sure what this function was meant to do.');
+ my $mfs = $self->get_all_MiscFeatures($mapset_name);
+ return (@$mfs > 0);
}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/StickyExon.pm b/modules/Bio/EnsEMBL/StickyExon.pm
index 3385756833..73ed0fba8b 100755
--- a/modules/Bio/EnsEMBL/StickyExon.pm
+++ b/modules/Bio/EnsEMBL/StickyExon.pm
@@ -13,49 +13,28 @@
=head1 NAME
-Bio::EnsEMBL::StickyExon - A Confirmed Exon which spans two or more contigs
- internally
-
-=head1 SYNOPSIS
-
- $sticky = new Bio::EnsEMBL::Exon;
-
- # is a normal exon
- $sticky->start();
- $sticky->end();
-
- # has component_Exons
- foreach $sub ( @{$sticky->get_all_component_Exons} ) {
- # $sub is an exon that ends on a contig
- }
+Bio::EnsEMBL::StickyExon - This is a deprecated class that will be entirely
+removed from the system. Do not use this class, use Bio::EnsEMBL::Exon instead
=head1 DESCRIPTION
-Sticky Exons represent Exons which internally span contigs.
-They are made during the write back on slices, which writes the exons that
-span joins into the database.
+StickyExons are deprecated. They should not be necessary and should not be
+used. This module will be entirely removed from EnsEMBL in the near future.
=head1 CONTACT
The EnsEMBL developer mailing list for questions : <ensembl-dev@ebi.ac.uk>
-=head1 APPENDIX
-
-The rest of the documentation details each of the object methods.
-Internal methods are usually preceded with a _
-
=cut
-# Let the code begin...
-
-
package Bio::EnsEMBL::StickyExon;
use Bio::Seq;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
-use vars qw(@ISA $AUTOLOAD);
+use vars qw(@ISA);
use strict;
# Object preamble - inherits from Bio::SeqFeature::Generic
@@ -65,7 +44,6 @@ use Bio::EnsEMBL::Exon;
@ISA = qw(Bio::EnsEMBL::Exon);
-# _initialize is where the heavy stuff will happen when new is called
sub new {
my($class,@args) = @_;
@@ -78,6 +56,9 @@ sub new {
# Array to store exon tags
$self->{_component_exons} = [];
+ deprecate("StickyExon is a deprecated class. It should not be needed." .
+ "Use normal Exons instead.");
+
return $self;
}
@@ -400,11 +381,7 @@ sub peptide {
#the peptide of this sticky is the region spanned by the component exons
my $pep_str = '';
if($pep_start && $pep_end) {
- #stop codon is trimmed off end so end may be past end of sequence
- my $pep = $tr->translate();
- my $len = $pep->length();
- $pep_end = $len if($len < $pep_end);
- $pep_str = $pep->subseq($pep_start, $pep_end);
+ $pep_str = $tr->translate->subseq($pep_start, $pep_end);
}
return Bio::Seq->new(-seq => $pep_str,
diff --git a/modules/Bio/EnsEMBL/Storable.pm b/modules/Bio/EnsEMBL/Storable.pm
new file mode 100644
index 0000000000..be1ae54cb6
--- /dev/null
+++ b/modules/Bio/EnsEMBL/Storable.pm
@@ -0,0 +1,171 @@
+#
+# EnsEMBL module for Bio::EnsEMBL::Storable
+#
+
+=head1 NAME
+
+Bio::EnsEMBL::Storable
+
+=head1 SYNOPSIS
+
+ my $dbID = $storable_object->dbID();
+ my $adaptor = $storable_object->adaptor();
+ if($storable_object->is_stored($db_adaptor))) {
+ ...
+ }
+=head1 DESCRIPTION
+
+This is a simple object which contains a few coordinate system attributes:
+name, internal identifier, version
+
+=head1 AUTHOR - Graham McVicker
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL development list ensembl-dev@ebi.ac.uk
+
+=head1 APPENDIX
+
+This is a storable base class. All objects which are storable in the database
+should inherit from this class. It provides two getter/setters: dbID()
+adaptor(). And a is_stored() method that can be used to determine if an
+object is already stored in a database.
+
+=cut
+
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::Storable;
+
+use Bio::EnsEMBL::Root;
+use vars qw(@ISA);
+
+#
+# will eventually remove unneeded inheritance to Root
+#
+@ISA = qw(Bio::EnsEMBL::Root);
+
+
+use Bio::EnsEMBL::Utils::Exception qw(throw);
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+sub new {
+ my $caller = shift;
+ my $class = ref($caller) || $caller;
+
+ my ($adaptor, $dbID) = rearrange(['ADAPTOR', 'dbID'],@_);
+
+ if($adaptor) {
+ if(!ref($adaptor) || !$adaptor->isa('Bio::EnsEMBL::DBSQL::BaseAdaptor')) {
+ throw('-ADAPTOR argument must be a Bio::EnsEMBL::DBSQL::BaseAdaptor');
+ }
+ }
+
+ return bless({'dbID' => $dbID, 'adaptor' => $adaptor}, $class);
+}
+
+
+=head2 dbID
+
+ Arg [1] : int $dbID
+ Example : none
+ Description: getter/setter for the database internal id
+ Returntype : int
+ Exceptions : none
+ Caller : general, set from adaptor on store
+
+=cut
+
+sub dbID {
+ my $self = shift;
+ $self->{'dbID'} = shift if(@_);
+ return $self->{'dbID'};
+}
+
+
+
+=head2 adaptor
+
+ Arg [1] : Bio::EnsEMBL::DBSQL::BaseAdaptor $adaptor
+ Example : none
+ Description: get/set for this objects Adaptor
+ Returntype : Bio::EnsEMBL::DBSQL::ChromsomeAdaptor
+ Exceptions : none
+ Caller : general, set from adaptor on store
+
+=cut
+
+sub adaptor {
+ my $self = shift;
+
+ if(@_) {
+ my $ad = shift;
+ if($ad && (!ref($ad) || !$ad->isa('Bio::EnsEMBL::DBSQL::BaseAdaptor'))) {
+ throw('Adaptor argument must be a Bio::EnsEMBL::DBSQL::BaseAdaptor');
+ }
+ $self->{'adaptor'} = $ad;
+ }
+
+ return $self->{'adaptor'}
+}
+
+
+=head2 is_stored
+
+ Arg [1] : Bio::EnsEMBL::DBSQL::DBConnection
+ Example : do_something if($object->is_stored($db));
+ Description: Returns true if this object is stored in the provided database.
+ This works under the assumption that if the adaptor and dbID are
+ set and the database of the adaptor shares the port, dbname and
+ hostname with the provided database, this object is stored in
+ that database.
+ Returntype : 1 or 0
+ Exceptions : throw if dbID is set but adaptor is not
+ throw if adaptor is set but dbID is not
+ throw if incorrect argument is passed
+ Caller : store methods
+
+=cut
+
+sub is_stored {
+ my $self = shift;
+ my $db = shift;
+
+ if(!$db || !ref($db) || !$db->isa('Bio::EnsEMBL::DBSQL::DBConnection')) {
+ throw('db argument must be a Bio::EnsEMBL::DBSQL::DBConnection');
+ }
+
+ my $adaptor = $self->{'adaptor'};
+ my $dbID = $self->{'dbID'};
+
+ if($dbID && !$adaptor) {
+ throw("Storable object has a dbID but not an adaptor.\n" .
+ 'Storable objects must have neither OR both.');
+ }
+
+ if($adaptor && !$dbID) {
+ throw("Storable object has an adaptor but not a dbID.\n".
+ "Storable objects must have neither OR both.");
+ }
+
+ return 0 if (!$adaptor && !dbID);
+
+ my $cur_db = $adaptor->db();
+
+ #
+ # Databases are the same if they share the same port, host and username
+ #
+ if($db->port == $cur_db->port &&
+ $db->host eq $cur_db->host &&
+ $db->dbname eq $cur_db->dbname) {
+ return 1;
+ }
+
+ return 0;
+}
+
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/Tile.pm b/modules/Bio/EnsEMBL/Tile.pm
index 9bdd90b7d8..05af48de22 100644
--- a/modules/Bio/EnsEMBL/Tile.pm
+++ b/modules/Bio/EnsEMBL/Tile.pm
@@ -10,16 +10,10 @@
=head1 NAME
-Bio::EnsEMBL::Tile - container objects for assembly style information
+Bio::EnsEMBL::Tile - DEPRECATED - You should not have to use this class.
+ The Bio::EnsEMBL::Slice::project method should be able
+ to construct any tiling path that you need.
-=head1 SYNOPSIS
-
- $tile_list = $slice->get_tiling_path()
-
-
-=head1 DESCRIPTION
-
- Each tile contains information about an ungapped sequence mapping from one sequence containing object to another.
=head1 AUTHOR - Arne Stabenau
@@ -30,32 +24,29 @@ This modules is part of the Ensembl project http://www.ensembl.org
Questions can be posted to the ensembl-dev mailing list:
ensembl-dev@ebi.ac.uk
-=head1 APPENDIX
-
-The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
-
=cut
-# Let the code begin...
-
-
package Bio::EnsEMBL::Tile;
use vars qw(@ISA);
use strict;
-# Object preamble - inherits from Bio::EnsEMBL::Root
use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Utils::Exception qw(deprecate);
@ISA = qw(Bio::EnsEMBL::Root);
+
### Only new_fast() is used by the EnsEMBL API
### Otter uses new()
sub new {
my $class = shift;
+ deprecate("Bio::EnsEMBL::Tile is a deprecated class.\nIf you want a " .
+ 'tiling path it is best to use Bio::EnsEMBL::Slice::project');
+
my $self = bless {}, $class;
if (@_) {
$self->throw("new does not take any arguments");
@@ -85,6 +76,9 @@ sub new {
=cut
sub new_fast {
+ deprecate("Bio::EnsEMBL::Tile is a deprecated class.\nIf you want a " .
+ 'tiling path it is best to use Bio::EnsEMBL::Slice::project');
+
# make it really fast
return bless {
'assembled_Seq' => $_[1],
diff --git a/modules/Bio/EnsEMBL/Transcript.pm b/modules/Bio/EnsEMBL/Transcript.pm
index bccaf7b389..2b44576cb5 100755
--- a/modules/Bio/EnsEMBL/Transcript.pm
+++ b/modules/Bio/EnsEMBL/Transcript.pm
@@ -23,16 +23,15 @@ up a gene transcript.
Creation:
my $tran = new Bio::EnsEMBL::Transcript();
- my $tran = new Bio::EnsEMBL::Transcript(@exons);
+ my $tran = new Bio::EnsEMBL::Transcript(-EXONS => \@exons);
Manipulation:
# Returns an array of Exon objects
- my @exons = @{$tran->get_all_Exons}
+ my @exons = @{$tran->get_all_Exons};
# Returns the peptide translation of the exons as a Bio::Seq
- my $pep = $tran->translate()
- # Sorts exons into order (forward for + strand, reverse for - strand)
- $tran->sort()
+ my $pep = $tran->translate();
+
=head1 CONTACT
@@ -44,33 +43,54 @@ package Bio::EnsEMBL::Transcript;
use vars qw(@ISA);
use strict;
-
-use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Feature;
use Bio::EnsEMBL::Exon;
use Bio::EnsEMBL::Translation;
-use Bio::EnsEMBL::TranscriptI;
use Bio::Tools::CodonTable;
use Bio::EnsEMBL::Mapper;
+use Bio::EnsEMBL::Utils::Argument qw( rearrange );
+use Bio::EnsEMBL::Utils::Exception qw( deprecate warning throw );
+
-@ISA = qw(Bio::EnsEMBL::Root Bio::EnsEMBL::TranscriptI);
+@ISA = qw(Bio::EnsEMBL::Feature);
sub new {
- my($class,@args) = @_;
+ my($class) = shift;
if( ref $class ) {
$class = ref $class;
}
- my $self = {};
- bless $self,$class;
+ my $self = $class->SUPER::new(@_);
- $self->{'_trans_exon_array'} = [];
+ my ( $exons, $stable_id, $version, $external_name, $external_db,
+ $external_status, $display_xref );
+
+ #catch for old style constructor calling:
+ if((@_ > 0) && ref($_[0])) {
+ $exons = [@_];
+ deprecate("Transcript constructor should use named arguments.\n" .
+ 'Use Bio::EnsEMBL::Transcript->new(-EXONS => \@exons);' .
+ "\ninstead of Bio::EnsEMBL::Transcript->new(\@exons);");
+ }
+ else {
+ ( $exons, $stable_id, $version, $external_name, $external_db,
+ $external_status, $display_xref ) =
+ rearrange( [ "EXONS", 'STABLE_ID', 'VERSION', 'EXTERNAL_NAME',
+ 'EXTERNAL_DB', 'EXTERNAL_STATUS', 'DISPLAY_XREF' ], @_ );
+ }
- # set stuff in self from @args
- foreach my $a (@args) {
- $self->add_Exon($a);
+ if( $exons ) {
+ $self->{'_trans_exon_array'} = $exons;
+ $self->recalculate_coordinates();
}
+ $self->stable_id( $stable_id );
+ $self->version( $version );
+ $self->external_name( $external_name ) if( defined $external_name );
+ $self->external_db( $external_db ) if( defined $external_db );
+ $self->external_status( $external_status ) if( defined $external_status );
+ $self->display_xref( $display_xref ) if( defined $display_xref );
return $self;
}
@@ -159,7 +179,7 @@ sub add_DBEntry {
my $dbe = shift;
unless($dbe && ref($dbe) && $dbe->isa('Bio::EnsEMBL::DBEntry')) {
- $self->throw('Expected DBEntry argument');
+ throw('Expected DBEntry argument');
}
$self->{'dbentries'} ||= [];
@@ -168,31 +188,6 @@ sub add_DBEntry {
-=head2 dbID
-
- Arg [1] : (optional) int $dbID
- A new value for the internal identifier of this transcript
- Example : $id = $transcript->dbID();
- Description: Getter/Setter for the internal identifier of this transcript
- Returntype : int
- Exceptions : none
- Caller : general
-
-=cut
-
-sub dbID {
- my $self = shift;
-
- if( @_ ) {
- my $value = shift;
- $self->{'dbID'} = $value;
- }
- return $self->{'dbID'};
-
-}
-
-
-
=head2 external_db
Title : external_db
@@ -207,11 +202,11 @@ sub external_db {
my ( $self, $ext_dbname ) = @_;
if(defined $ext_dbname) {
- return ( $self->{'_ext_dbname'} = $ext_dbname );
+ return ( $self->{'external_db'} = $ext_dbname );
}
- if( exists $self->{'_ext_dbname'} ) {
- return $self->{'_ext_dbname'};
+ if( exists $self->{'external_db'} ) {
+ return $self->{'external_db'};
}
my $display_xref = $self->display_xref();
@@ -239,11 +234,11 @@ sub external_status {
my ( $self, $ext_status ) = @_;
if(defined $ext_status) {
- return ( $self->{'_ext_status'} = $ext_status );
+ return ( $self->{'external_status'} = $ext_status );
}
- if( exists $self->{'_ext_status'} ) {
- return $self->{'_ext_status'};
+ if( exists $self->{'external_status'} ) {
+ return $self->{'external_status'};
}
my $display_xref = $self->display_xref();
@@ -273,11 +268,11 @@ sub external_name {
my ($self, $ext_name) = @_;
if(defined $ext_name) {
- return ( $self->{'_ext_name'} = $ext_name );
+ return ( $self->{'external_name'} = $ext_name );
}
- if( exists $self->{'_ext_name'} ) {
- return $self->{'_ext_name'};
+ if( exists $self->{'external_name'} ) {
+ return $self->{'external_name'};
}
my $display_xref = $self->display_xref();
@@ -290,32 +285,28 @@ sub external_name {
}
+=head2 is_known
+
+ Args : none
+ Example : none
+ Description: returns true if this transcript has a display_xref
+ Returntype : 0,1
+ Exceptions : none
+ Caller : general
+
+=cut
+
sub is_known {
my $self = shift;
- for my $entry ( @{$self->get_all_DBLinks()} ) {
- return 1 if uc($entry->status) eq "KNOWN" ;
- }
- return 0;
+ return ($self->{'display_xref'}) ? 1 : 0;
}
sub type {
- my ($self, $type) = @_;
-
- if(defined $type) {
- $self->{'_type'} = $type;
- }
+ my $self = shift;
- return $self->{'_type'};
-}
-
-sub adaptor {
- my $self = shift;
-
- if( @_ ) {
- $self->{'adaptor'} = shift;
- }
- return $self->{'adaptor'};
+ $self->{'type'} = shift if( @_ );
+ return $self->{'type'};
}
@@ -323,7 +314,7 @@ sub adaptor {
Arg [1] : Bio::EnsEMBL::DBEntry $display_xref
Example : $transcript->display_xref(Bio::EnsEMBL::DBEntry->new(...));
- Description: get/set/lazy_loaded display_xref for this transcript
+ Description: getter setter for display_xref for this transcript
Returntype : Bio::EnsEMBL::DBEntry
Exceptions : none
Caller : general
@@ -331,42 +322,9 @@ sub adaptor {
=cut
sub display_xref {
-
- my $self = shift;
- if( @_ ) {
- $self->{'display_xref'} = shift;
- } elsif( exists $self->{'display_xref'} ) {
- return $self->{'display_xref'};
- } elsif ( defined $self->adaptor() ) {
- $self->{'display_xref'} = $self->adaptor->get_display_xref( $self );
- }
-
- return $self->{'display_xref'};
-}
-
-
-
-
-=head2 _translation_id
-
- Title : _translation_id
- Usage : $obj->_translation_id($newval)
- Function:
- Returns : translation objects dbID
- Args : newvalue (optional)
-
-
-=cut
-
-sub _translation_id {
- my $self = shift;
-
- if( @_ ) {
- my $value = shift;
- $self->{'_translation_id'} = $value;
- }
- return $self->{'_translation_id'};
-
+ my $self = shift;
+ $self->{'display_xref'} = shift if(@_);
+ return $self->{'display_xref'};
}
@@ -386,73 +344,17 @@ sub translation {
if( @_ ) {
my $value = shift;
if( ! ref $value || !$value->isa('Bio::EnsEMBL::Translation') ) {
- $self->throw("This [$value] is not a translation");
+ throw("This [$value] is not a translation");
}
$self->{'translation'} = $value;
- $self->{'_translation_id'} = $value->dbID();
- } else {
- if( ! defined $self->{'translation'} &&
- defined $self->_translation_id() ) {
- $self->{'translation'} =
- $self->adaptor->db->get_TranslationAdaptor->fetch_by_dbID(
- $self->_translation_id(), $self );
- }
+ } elsif( !exists $self->{'translation'} and defined $self->adaptor() ) {
+ $self->{'translation'} =
+ $self->adaptor()->db()->get_TranslationAdaptor()->
+ fetch_by_Transcript( $self );
}
return $self->{'translation'};
}
-=head2 start
-
- Description: it returns the start coordinate of the lef-most exon, i.e.
- the 5prime exon in the forward strand and the 3prime exon in the reverse strand
-
-=cut
-
-
-sub start {
- my $self = shift;
- my $arg = shift;
-
- my $strand;
- my $start;
- if( defined $arg ) {
- $self->{'_start'} = $arg;
- } elsif(! defined $self->{'_start'} ) {
-
- $strand = $self->start_Exon->strand();
- if( $strand == 1 ) {
- $start = $self->start_Exon->start();
- } else {
- $start = $self->end_Exon->start();
- }
- $self->{'_start'} = $start;
- }
-
- return $self->{'_start'};
-}
-
-
-sub end {
- my $self = shift;
- my $arg = shift;
-
- my $strand;
- my $end
-;
- if( defined $arg ) {
- $self->{'_end'} = $arg;
- } elsif( ! defined $self->{'_end'} ) {
- $strand = $self->start_Exon->strand();
- if( $strand == 1 ) {
- $end = $self->end_Exon->end();
- } else {
- $end = $self->start_Exon->end();
- }
- $self->{'_end'} = $end;
- }
-
- return $self->{'_end'};
-}
=head2 spliced_seq
@@ -482,10 +384,14 @@ sub spliced_seq {
=head2 translateable_seq
Args : none
- Example : none
- Description: returns a string with the translateable part of the
- Sequence. It magically pads the exon sequences with
- N if the phases of the Exons dont align
+ Example : print $transcript->translateable_seq(), "\n";
+ Description: Returns a sequence string which is the the translateable part
+ of the transcripts sequence. This is formed by concatenating
+ the coding portion of the exons sequence together (UTRs are not
+ included in the sequence). Exon phase information is ignored
+ unless the MONKEY_EXONS environment variable is set with a true
+ value. If MONKEY_EXONS is true the sequence may be padded with
+ Ns in order to acheive correct exon phases.
Returntype : txt
Exceptions : none
Caller : general
@@ -515,9 +421,9 @@ sub translateable_seq {
if( $phase != $lastphase && ( defined $ENV{'MONKEY_EXONS'})) {
# endpadding for the last exon
if( $lastphase == 1 ) {
- $mrna .= 'NN';
+ $mrna .= 'NN';
} elsif( $lastphase == 2 ) {
- $mrna .= 'N';
+ $mrna .= 'N';
}
#startpadding for this exon
$mrna .= 'N' x $phase;
@@ -557,20 +463,17 @@ sub cdna_coding_start {
my @exons = @{$self->get_all_Exons};
my $exon;
- my $found = 0;
while($exon = shift @exons) {
if($exon == $self->translation->start_Exon) {
- #add the utr portion of the start exon
- $start += $self->translation->start;
- $found = 1;
- last;
+ #add the utr portion of the start exon
+ $start += $self->translation->start;
+ last;
} else {
- #add the entire length of this non-coding exon
- $start += $exon->length;
+ #add the entire length of this non-coding exon
+ $start += $exon->length;
}
}
- $self->throw("Start exon could not be found") if(!$found);
$self->{'cdna_coding_start'} = $start;
}
@@ -626,11 +529,11 @@ sub cdna_coding_end {
Example : $coding_region_start = $transcript->coding_region_start
Description: Retrieves the start of the coding region of this transcript
in genomic coordinates (i.e. in either slice or contig coords).
- By convention, the coding_region_start is always lower than
- the value returned by the coding_end method.
- The value returned by this function is NOT the biological
- coding start since on the reverse strand the biological coding
- start would be the higher genomic value.
+ By convention, the coding_region_start is always lower than
+ the value returned by the coding_end method.
+ The value returned by this function is NOT the biological
+ coding start since on the reverse strand the biological coding
+ start would be the higher genomic value.
Returntype : int
Exceptions : none
Caller : general
@@ -720,15 +623,44 @@ sub add_Exon{
#yup - we are going to be picky here...
unless(defined $exon && ref $exon && $exon->isa("Bio::EnsEMBL::Exon") ) {
- $self->throw("[$exon] is not a Bio::EnsEMBL::Exon!");
+ throw("[$exon] is not a Bio::EnsEMBL::Exon!");
}
- #invalidate the start, end and strand - they may need to be recalculated
- $self->{'_start'} = undef;
- $self->{'_end'} = undef;
- $self->{'_strand'} = undef;
-
- push(@{$self->{'_trans_exon_array'}},$exon);
+
+ $self->{'_trans_exon_array'} ||= [];
+
+ my $ea = $self->{'_trans_exon_array'};
+ if( @$ea ) {
+ if( $exon->strand() == 1 ) {
+ if( $exon->start() > $ea->[$#$ea]->end() ) {
+ push(@{$self->{'_trans_exon_array'}},$exon);
+ } else {
+ # insert it at correct place
+ for( my $i=0; $i <= $#$ea; $i++ ) {
+ if( $exon->end() < $ea->[$i]->start() ) {
+ splice( @$ea, $i, 0, $exon );
+ last;
+ }
+ }
+ }
+ } else {
+ if( $exon->end() < $ea->[$#$ea]->start() ) {
+ push(@{$self->{'_trans_exon_array'}},$exon);
+ } else {
+ # insert it at correct place
+ for( my $i=0; $i <= $#$ea; $i++ ) {
+ if( $exon->start() > $ea->[$i]->end() ) {
+ splice( @$ea, $i, 0, $exon );
+ last;
+ }
+ }
+ }
+ }
+ } else {
+ push( @$ea, $exon );
+ }
+ # recalculate start, end, slice, strand
+ $self->recalculate_coordinates();
}
@@ -748,7 +680,10 @@ sub add_Exon{
sub get_all_Exons {
my ($self) = @_;
-
+ if( ! defined $self->{'_trans_exon_array'} && defined $self->adaptor() ) {
+ $self->{'_trans_exon_array'} = $self->adaptor()->db()->
+ get_ExonAdaptor()->fetch_all_by_Transcript( $self );
+ }
return $self->{'_trans_exon_array'};
}
@@ -841,24 +776,25 @@ sub get_all_peptide_variations {
foreach my $allele (@alleles) {
next if $allele eq '-'; #skip deletions
next if CORE::length($allele) != 1; #skip insertions
-
-# if($strand == -1) {
- #complement the allele if the snp is on the reverse strand
-# $allele =~
-# tr/acgtrymkswhbvdnxACGTRYMKSWHBVDNX/tgcayrkmswdvbhnxTGCAYRKMSWDVBHNX/;
-# }
+
+ #get_all_cdna_SNPs always gives strand of 1 now
+ #if($strand == -1) {
+ # #complement the allele if the snp is on the reverse strand
+ # $allele =~
+ # tr/acgtrymkswhbvdnxACGTRYMKSWHBVDNX/tgcayrkmswdvbhnxTGCAYRKMSWDVBHNX/;
+ #}
#create a data structure of variant alleles sorted by both their
#peptide position and their position within the peptides codon
$variant_alleles ||= {};
if(exists $variant_alleles->{$peptide}) {
- my $alleles_arr = $variant_alleles->{$peptide}->[1];
- push @{$alleles_arr->[$codon_pos]}, $allele;
+ my $alleles_arr = $variant_alleles->{$peptide}->[1];
+ push @{$alleles_arr->[$codon_pos]}, $allele;
} else {
- #create a list of 3 lists (one list for each codon position)
- my $alleles_arr = [[],[],[]];
- push @{$alleles_arr->[$codon_pos]}, $allele;
- $variant_alleles->{$peptide} = [$codon, $alleles_arr];
+ #create a list of 3 lists (one list for each codon position)
+ my $alleles_arr = [[],[],[]];
+ push @{$alleles_arr->[$codon_pos]}, $allele;
+ $variant_alleles->{$peptide} = [$codon, $alleles_arr];
}
}
}
@@ -878,13 +814,13 @@ sub get_all_peptide_variations {
foreach my $a1 (@{$alleles->[0]}) {
substr($codon, 0, 1) = $a1;
foreach my $a2 (@{$alleles->[1]}) {
- substr($codon, 1, 1) = $a2;
- foreach my $a3 (@{$alleles->[2]}) {
- substr($codon, 2, 1) = $a3;
- my $aa = $codon_table->translate($codon);
- #print "$codon translation is $aa\n";
- $alt_amino_acids{$aa} = 1;
- }
+ substr($codon, 1, 1) = $a2;
+ foreach my $a3 (@{$alleles->[2]}) {
+ substr($codon, 2, 1) = $a3;
+ my $aa = $codon_table->translate($codon);
+ #print "$codon translation is $aa\n";
+ $alt_amino_acids{$aa} = 1;
+ }
}
}
@@ -895,6 +831,7 @@ sub get_all_peptide_variations {
return \%out;
}
+
=head2 get_all_SNPs
Arg [1] : (optional) int $flanking
@@ -921,26 +858,16 @@ sub get_all_peptide_variations {
sub get_all_SNPs {
my $self = shift;
- my $flanking = shift || 0;
+ my $flanking = shift;
my %snp_hash;
my $sa = $self->adaptor->db->get_SliceAdaptor;
#retrieve a slice in the region of the transcript
- my $slice = $sa->fetch_by_transcript_id($self->dbID, $flanking);
+ my $slice = $sa->fetch_by_transcript_id($self->dbID, $flanking );
#copy this transcript, so we can work in coord system we are interested in
- my $transcript = Bio::EnsEMBL::Transcript->new;
- %$transcript = %$self;
- delete $transcript->{'translation'};
-
- #transform transcript to same coord system we will get snps in
- my %exon_transforms;
- foreach my $exon (@{$transcript->get_all_Exons}) {
- my $new_exon = $exon->transform($slice);
- $exon_transforms{$exon} = $new_exon;
- }
- $transcript->transform(\%exon_transforms);
+ my $transcript = $self->transfer( $slice );
#get all of the snps in the transcript region
my $snps = $slice->get_all_SNPs;
@@ -968,40 +895,40 @@ sub get_all_SNPs {
else {
#snp is inside transcript region check if it overlaps an exon
foreach my $e (@{$transcript->get_all_Exons}) {
- if($snp->end >= $e->start && $snp->start <= $e->end) {
- #this snp is in an exon
-
- if(($trans_strand == 1 &&
- $snp->end < $transcript->coding_region_start) ||
- ($trans_strand == -1 &&
- $snp->start > $transcript->coding_region_end)) {
- #this snp is in the 5' UTR
- $key = 'five prime UTR';
- }
-
- elsif(($trans_strand == 1 &&
- $snp->start > $transcript->coding_region_end)||
- ($trans_strand == -1 &&
- $snp->end < $transcript->coding_region_start)) {
- #this snp is in the 3' UTR
- $key = 'three prime UTR';
- }
-
- else {
- #snp is coding
- $key = 'coding';
- }
- last;
- }
+ if($snp->end >= $e->start && $snp->start <= $e->end) {
+ #this snp is in an exon
+
+ if(($trans_strand == 1 &&
+ $snp->end < $transcript->coding_region_start) ||
+ ($trans_strand == -1 &&
+ $snp->start > $transcript->coding_region_end)) {
+ #this snp is in the 5' UTR
+ $key = 'five prime UTR';
+ }
+
+ elsif(($trans_strand == 1 &&
+ $snp->start > $transcript->coding_region_end)||
+ ($trans_strand == -1 &&
+ $snp->end < $transcript->coding_region_start)) {
+ #this snp is in the 3' UTR
+ $key = 'three prime UTR';
+ }
+
+ else {
+ #snp is coding
+ $key = 'coding';
+ }
+ last;
+ }
}
unless($key) {
- #snp was not in an exon and is therefore intronic
- $key = 'intronic';
+ #snp was not in an exon and is therefore intronic
+ $key = 'intronic';
}
}
unless($key) {
- #$self->warn('SNP could not be mapped. In/Dels not supported yet...');
+ #warning('SNP could not be mapped. In/Dels not supported yet...');
next;
}
@@ -1041,63 +968,54 @@ sub get_all_cdna_SNPs {
my $all_snps = $self->get_all_SNPs;
my %snp_hash;
- my @cdna_types = ('three prime UTR',
- 'five prime UTR',
- 'coding');
+ my @cdna_types = ('three prime UTR', 'five prime UTR','coding');
my $sa = $self->adaptor->db->get_SliceAdaptor;
my $slice = $sa->fetch_by_transcript_id($self->dbID);
#copy this transcript, so we can work in coord system we are interested in
- my $transcript = Bio::EnsEMBL::Transcript->new;
- %$transcript = %$self;
- delete $transcript->{'translation'};
-
- #transform transcript to same coord system we will get snps in
- my %exon_transforms;
- my $new_exon ;
- foreach my $exon (@{$transcript->get_all_Exons}) {
- $new_exon = $exon->transform($slice);
- $exon_transforms{$exon} = $new_exon;
- }
- $transcript->transform(\%exon_transforms);
+ my $transcript = $self->transfer($slice);
foreach my $type (@cdna_types) {
$snp_hash{$type} = [];
foreach my $snp (@{$all_snps->{$type}}) {
- my @coords =
- $transcript->genomic2cdna($snp->start,
- $snp->end,
- $snp->strand,
- $slice);
+ my @coords = $transcript->genomic2cdna($snp->start, $snp->end,
+ $snp->strand, $slice);
#skip snps that don't map cleanly (possibly an indel...)
if(scalar(@coords) != 1) {
- #$self->warn("snp of type $type does not map cleanly\n");
- next;
+ #warning("snp of type $type does not map cleanly\n");
+ next;
}
my ($coord) = @coords;
unless($coord->isa('Bio::EnsEMBL::Mapper::Coordinate')) {
- #$self->warn("snp of type $type maps to gap\n");
- next;
+ #warning("snp of type $type maps to gap\n");
+ next;
}
- # change allele and ambicode if strand of coordinate system is different to snp strand
-
- if ($coord->strand() == -1){
- $snp->{'alleles'} =~ tr/acgthvmrdbkynwsACGTDBKYHVMRNWS\//tgcadbkyhvmrnwsTGCAHVMRDBKYNWS\//;
- $snp->{'_ambiguity_code'} =~ tr/acgthvmrdbkynwsACGTDBKYHVMRNWS\//tgcadbkyhvmrnwsTGCAHVMRDBKYNWS\//;
- }
+ my $alleles = $snp->{'alleles'};
+ my $ambicode = $snp->{'_ambiguity_code'};
+
+ #we arbitrarily put the SNP on the +ve strand because it is easier to
+ #work with in the webcode.
+ if($coord->strand == -1) {
+ $alleles =~
+ tr/acgthvmrdbkynwsACGTDBKYHVMRNWS\//tgcadbkyhvmrnwsTGCAHVMRDBKYNWS\//;
+ $ambicode =~
+ tr/acgthvmrdbkynwsACGTDBKYHVMRNWS\//tgcadbkyhvmrnwsTGCAHVMRDBKYNWS\//;
+ }
- #copy the snp and convert to cdna coords, we arbitrarily move snps to +ve strand
+ #copy the snp and convert to cdna coords...
my $new_snp;
%$new_snp = %$snp;
bless $new_snp, ref $snp;
$new_snp->start($coord->start);
$new_snp->end($coord->end);
$new_snp->strand(1);
+ $new_snp->{'alleles'} = $alleles;
+ $new_snp->{'_ambiguity_code'} = $ambicode;
push @{$snp_hash{$type}}, $new_snp;
}
}
@@ -1125,10 +1043,9 @@ sub flush_Exons{
$self->{'coding_region_end'} = undef;
$self->{'cdna_coding_start'} = undef;
$self->{'cdna_coding_end'} = undef;
- $self->{'_start'} = undef;
- $self->{'_end'} = undef;
- $self->{'_strand'} = undef;
- $self->{'_exon_coord_mapper'} = undef;
+ $self->{'start'} = undef;
+ $self->{'end'} = undef;
+ $self->{'strand'} = undef;
$self->{'_trans_exon_array'} = [];
}
@@ -1196,7 +1113,7 @@ sub get_all_translateable_Exons {
my ( $self ) = @_;
my $translation = $self->translation
- or $self->throw("No translation attached to transcript object");
+ or throw("No translation attached to transcript object");
my $start_exon = $translation->start_Exon;
my $end_exon = $translation->end_Exon;
my $t_start = $translation->start;
@@ -1217,7 +1134,7 @@ sub get_all_translateable_Exons {
# Adjust to translation start if this is the start exon
if ($ex == $start_exon ) {
if ($t_start < 1 or $t_start > $length) {
- $self->throw("Translation start '$t_start' is outside exon $ex length=$length");
+ throw("Translation start '$t_start' is outside exon $ex length=$length");
}
$adjust_start = $t_start - 1;
}
@@ -1225,7 +1142,7 @@ sub get_all_translateable_Exons {
# Adjust to translation end if this is the end exon
if ($ex == $end_exon) {
if ($t_end < 1 or $t_end > $length) {
- $self->throw("Translation end '$t_end' is outside exon $ex length=$length");
+ throw("Translation end '$t_end' is outside exon $ex length=$length");
}
$adjust_end = $t_end - $length;
}
@@ -1270,10 +1187,11 @@ sub translate {
my $display_id;
if( defined $self->translation->stable_id ) {
$display_id = $self->translation->stable_id;
- } elsif ( defined $self->temporary_id ) {
- $display_id = $self->temporary_id;
+ } elsif ( defined $self->translation->dbID ) {
+ $display_id = $self->translation->dbID();
} else {
- $display_id = $self->translation->dbID;
+ #use memory location as temp id
+ $display_id = scalar($self->translation());
}
if( CORE::length( $mrna ) % 3 == 0 ) {
@@ -1284,12 +1202,12 @@ sub translate {
# won't have a terminal stop codon
# if you want to have a terminal stop codon either comment this line out
# or call translatable seq directly and produce a translation from it
-
+
my $peptide = Bio::Seq->new( -seq => $mrna,
-moltype => "dna",
-alphabet => 'dna',
-id => $display_id );
-
+
return $peptide->translate;
}
@@ -1322,43 +1240,6 @@ sub seq {
-
-=head2 sort
-
- Title : sort
- Usage : $feat->sort()
- Function: Sorts the exon features by start coordinate
- Sorts forward for forward strand and reverse for reverse strand
- Returns : none
- Args : none
-
-=cut
-
-sub sort {
- my $self = shift;
-
- # Fetch all the features
- my @exons = @{$self->get_all_Exons()};
-
- # Empty the feature table
- $self->flush_Exons();
-
- # Now sort the exons and put back in the feature table
- my $strand = $exons[0]->strand;
-
- if ($strand == 1) {
- @exons = sort { $a->start <=> $b->start } @exons;
- } elsif ($strand == -1) {
- @exons = sort { $b->start <=> $a->start } @exons;
- }
-
- foreach my $e (@exons) {
- $self->add_Exon($e);
- }
-}
-
-
-
=head1 pep2genomic
Arg 1 : integer start - relative to peptide
@@ -1375,7 +1256,7 @@ sub pep2genomic {
my ($self,$start,$end) = @_;
if( !defined $end ) {
- $self->throw("Must call with start/end");
+ throw("Must call with start/end");
}
# move start end into translate cDNA coordinates now.
@@ -1418,7 +1299,7 @@ sub genomic2pep {
my ($self, $start, $end, $strand, $contig) = @_;
unless(defined $start && defined $end && defined $strand) {
- $self->throw("start, end and strand arguments are required");
+ throw("start, end and strand arguments are required");
}
my @coords = $self->genomic2cdna($start, $end, $strand, $contig);
@@ -1521,7 +1402,7 @@ sub cdna2genomic {
my ($self,$start,$end) = @_;
if( !defined $end ) {
- $self->throw("Must call with start/end");
+ throw("Must call with start/end");
}
my $mapper = $self->_get_cdna_coord_mapper();
@@ -1561,22 +1442,31 @@ sub cdna2genomic {
=cut
sub genomic2cdna {
- my ($self, $start, $end, $strand, $contig) = @_;
+ my ($self, $start, $end, $strand, $slice) = @_;
unless(defined $start && defined $end && defined $strand) {
- $self->throw("start, end and strand arguments are required\n");
+ throw("start, end and strand arguments are required\n");
}
#"ids" in mapper are contigs of exons, so use the same contig that should
#be attached to all of the exons...
- $contig = $self->get_all_Exons->[0]->contig unless(defined $contig);
- my $mapper = $self->_get_cdna_coord_mapper;
+ if( $slice ) {
+ throw( "Arbitrary coordinates not supported yet" );
+ if( ! $self->adaptor() ) {
+ throw( "Cant do genomic2cdna without database connection" );
+ }
+ my $asm_mapper_adaptor = $self->adaptor()->db()->get_AssemblyMapperAdaptor();
+ # map from given slice coord system into $self->slice() ...
+ }
+ my $mapper = $self->_get_cdna_coord_mapper;
+
+
#print "MAPPING $start - $end ($strand)\n";
#print $contig->name . "=" . $self->get_all_Exons->[0]->contig->name . "\n";
-
- return $mapper->map_coordinates($contig, $start, $end, $strand, "genomic");
+ $slice = $self->slice();
+ return $mapper->map_coordinates($slice, $start, $end, $strand, "genomic");
}
@@ -1630,7 +1520,7 @@ sub _get_cdna_coord_mapper {
sub start_Exon{
my ($self,@args) = @_;
- return ${$self->{'_trans_exon_array'}}[0];
+ return $self->get_all_Exons()->[0];
}
=head2 end_Exon
@@ -1645,51 +1535,7 @@ sub start_Exon{
sub end_Exon{
my ($self,@args) = @_;
- return ${$self->{'_trans_exon_array'}}[$#{$self->{'_trans_exon_array'}}];
-}
-
-
-=head2 created
-
- Title : created
- Usage : $obj->created($newval)
- Function:
- Returns : value of created
- Args : newvalue (optional)
-
-
-=cut
-
-sub created{
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'created'} = $value;
- }
- return $obj->{'created'};
-
-}
-
-
-=head2 modified
-
- Title : modified
- Usage : $obj->modified($newval)
- Function:
- Returns : value of modified
- Args : newvalue (optional)
-
-
-=cut
-
-sub modified{
- my $obj = shift;
- if( @_ ) {
- my $value = shift;
- $obj->{'modified'} = $value;
- }
- return $obj->{'modified'};
-
+ return $self->get_all_Exons()->[-1];
}
@@ -1728,22 +1574,11 @@ sub description{
=cut
sub version{
+ my $self = shift;
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_version'} = $value;
- }
-
- if( exists $self->{'_version'} ) {
- return $self->{'_version'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_version'};
+ $self->{'version'} = shift if( @_ );
+ return $self->{'version'};
}
@@ -1759,179 +1594,365 @@ sub version{
=cut
sub stable_id{
+ my $self = shift;
- my ($self,$value) = @_;
-
+ $self->{'stable_id'} = shift if( @_ );
+
+ return $self->{'stable_id'};
+}
+
+
+
+=head2 swap_exons
+
+ Arg [1] : Bio::EnsEMBL::Exon $old_Exon
+ An exon that should be replaced
+ Arg [2] : Bio::EnsEMBL::Exon $new_Exon
+ The replacement Exon
+ Example : none
+ Description: exchange an exon in the current Exon list with a given one.
+ Usually done before storing of Gene, so the Exons can
+ be shared between Transcripts.
+ Returntype : none
+ Exceptions : none
+ Caller : GeneAdaptor->store()
- if( defined $value ) {
- $self->{'_stable_id'} = $value;
- return;
+=cut
+
+sub swap_exons {
+ my ( $self, $old_exon, $new_exon ) = @_;
+
+ my $arref = $self->{'_trans_exon_array'};
+ for(my $i = 0; $i < @$arref; $i++) {
+ if($arref->[$i] == $old_exon) {
+ $arref->[$i] = $new_exon;
+ last;
}
+ }
- if( exists $self->{'_stable_id'} ) {
- return $self->{'_stable_id'};
+ if( defined $self->{'translation'} ) {
+ if( $self->translation()->start_Exon() == $old_exon ) {
+ $self->translation()->start_Exon( $new_exon );
+ }
+ if( $self->translation()->end_Exon() == $old_exon ) {
+ $self->translation()->end_Exon( $new_exon );
}
+ }
+}
- $self->_get_stable_entry_info();
+=head2 transform
- return $self->{'_stable_id'};
+ Arg 1 : String $coordinate_system_name
+ Arg [2] : String $coordinate_system_version
+ Example : $transcript = $transcript->transform('contig');
+ $transcript = $transcript->transform('chromosome', 'NCBI33');
+ Description: Moves this Transcript to the given coordinate system.
+ If this Transcript has Exons attached, they move as well.
+ A new Transcript is returned. If the transcript cannot be
+ transformed to the destination coordinate system undef is
+ returned instead.
+ Returntype : Bio::EnsEMBL::Transcript
+ Exceptions : wrong parameters
+ Caller : general
-}
+=cut
-sub _get_stable_entry_info {
- my $self = shift;
- if( !defined $self->adaptor ) {
- return undef;
- }
+sub transform {
+ my $self = shift;
+
+ # catch for old style transform calls
+ if( ref $_[0] eq 'HASH') {
+ deprecate("Calling transform with a hashref is deprecate.\n" .
+ 'Use $trans->transfer($slice) or ' .
+ '$trans->transform("coordsysname") instead.');
+ my (undef, $new_ex) = each(%{$_[0]});
+ return $self->transfer($new_ex->slice);
+ }
+
+ my $new_transcript = $self->SUPER::transform( @_ );
+ return undef unless $new_transcript;
- $self->adaptor->get_stable_entry_info($self);
+ if( defined $self->{'translation'} ) {
+ my $new_translation;
+ %$new_translation = %{$self->{'translation'}};;
+ bless $new_translation, ref( $self->{'translation'} );
+ $new_transcript->{'translation'} = $new_translation;
+ }
+ if( exists $self->{'_trans_exon_array'} ) {
+ my @new_exons;
+ for my $old_exon ( @{$self->{'_trans_exon_array'}} ) {
+ my $new_exon = $old_exon->transform( @_ );
+ if( defined $new_transcript->{'translation'} ) {
+ if( $new_transcript->translation()->start_Exon() == $old_exon ) {
+ $new_transcript->translation()->start_Exon( $new_exon );
+ }
+ if( $new_transcript->translation()->end_Exon() == $old_exon ) {
+ $new_transcript->translation()->end_Exon( $new_exon );
+ }
+ }
+ push( @new_exons, $new_exon );
+ }
+ $new_transcript->{'_trans_exon_array'} = \@new_exons;
+ }
+
+ #flush internal values that depend on the exon coords that may have been
+ #cached
+ $new_transcript->{'_exon_coord_mapper'} = undef;
+ $new_transcript->{'coding_region_start'} = undef;
+ $new_transcript->{'coding_region_end'} = undef;
+ $new_transcript->{'cdna_coding_start'} = undef;
+ $new_transcript->{'cdna_coding_end'} = undef;
+
+ return $new_transcript;
}
-=head2 temporary_id
- Title : temporary_id
- Usage : $obj->temporary_id($newval)
- Function: Temporary ids are used for Genscan predictions - which should probably
- be moved over to being stored inside the gene tables anyway. Bio::EnsEMBL::TranscriptFactory use this
- Example :
- Returns : value of temporary_id
- Args : newvalue (optional)
+=head2 transfer
+ Arg 1 : Bio::EnsEMBL::Slice $destination_slice
+ Example : $transcript = $transcript->transfer($slice);
+ Description: Moves this transcript to the given slice.
+ If this Transcripts has Exons attached, they move as well.
+ Returntype : Bio::EnsEMBL::Transcript
+ Exceptions : none
+ Caller : general
=cut
-sub temporary_id{
- my ($obj,$value) = @_;
- if( defined $value) {
- $obj->{'temporary_id'} = $value;
+
+sub transfer {
+ my $self = shift;
+
+ my $new_transcript = $self->SUPER::transfer( @_ );
+ return undef unless $new_transcript;
+
+ if( defined $self->{'translation'} ) {
+ my $new_translation;
+ %$new_translation = %{$self->{'translation'}};;
+ bless $new_translation, ref( $self->{'translation'} );
+ $new_transcript->{'translation'} = $new_translation;
+ }
+
+ if( exists $self->{'_trans_exon_array'} ) {
+ my @new_exons;
+ for my $old_exon ( @{$self->{'_trans_exon_array'}} ) {
+ my $new_exon = $old_exon->transfer( @_ );
+ if( defined $new_transcript->{'translation'} ) {
+ if( $new_transcript->translation()->start_Exon() == $old_exon ) {
+ $new_transcript->translation()->start_Exon( $new_exon );
+ }
+ if( $new_transcript->translation()->end_Exon() == $old_exon ) {
+ $new_transcript->translation()->end_Exon( $new_exon );
+ }
+ }
+ push( @new_exons, $new_exon );
}
- return $obj->{'temporary_id'};
-}
+ $new_transcript->{'_trans_exon_array'} = \@new_exons;
+ }
+ #flush internal values that depend on the exon coords that may have been
+ #cached
+ $new_transcript->{'_exon_coord_mapper'} = undef;
+ $new_transcript->{'coding_region_start'} = undef;
+ $new_transcript->{'coding_region_end'} = undef;
+ $new_transcript->{'cdna_coding_start'} = undef;
+ $new_transcript->{'cdna_coding_end'} = undef;
+ return $new_transcript;
+}
-=head2 transform
- Arg 1 : hashref $old_new_exon_map
- a hash that maps old to new exons for a whole gene
- Function : maps transcript in place to different coordinate system,
- It does so by replacing its old exons with new ones.
- Returntype: none
- Exceptions: none
- Caller : Gene->transform()
+=head recalculate_coordinates
-=cut
+ Args : none
+ Example : none
+ Description: called when exon coordinate change happened to recalculate the
+ coords of the transcript. This method should be called if one
+ of the exons has been changed.
+ Returntype : none
+ Exceptions : none
+ Caller : internal
+=cut
-sub transform {
+sub recalculate_coordinates {
my $self = shift;
- my $href_exons = shift;
- my @mapped_list_of_exons;
- foreach my $exon (@{$self->get_all_Exons()}) {
- # the old exon was successfully remapped then store the new exon
- if ( exists $$href_exons{$exon} ) {
- push @mapped_list_of_exons, $$href_exons{$exon};
+ my $exons = $self->get_all_Exons();
+
+ return if(!$exons || !@$exons);
+
+ my ( $slice, $start, $end, $strand );
+ $slice = $exons->[0]->slice();
+ $strand = $exons->[0]->strand();
+ $start = $exons->[0]->start();
+ $end = $exons->[0]->end();
+
+ my $transsplicing = 0;
+
+ for my $e ( @$exons ) {
+ if( $e->start() < $start ) {
+ $start = $e->start();
}
- # but for the case where the exon was unable to be mapped, as it
- # was outside the bounds of the slice, include the original exon.
- else {
- push @mapped_list_of_exons, $exon;
+
+ if( $e->end() > $end ) {
+ $end = $e->end();
+ }
+
+ if( $slice && $e->slice() && $e->slice()->name() ne $slice->name() ) {
+ throw( "Exons with different slices not allowed on one Transcript" );
+ }
+
+ if( $e->strand() != $strand ) {
+ $transsplicing = 1;
}
}
+ if( $transsplicing ) {
+ warning( "Transcript contained trans splicing event" );
+ }
- #flush the exons and all related internal caches
- $self->flush_Exons();
+ $self->start( $start );
+ $self->end( $end );
+ $self->strand( $strand );
+ $self->slice( $slice );
+
+ #flush internal values that depend on the exon coords that may have been
+ #cached
+ $self->{'_exon_coord_mapper'} = undef;
+ $self->{'coding_region_start'} = undef;
+ $self->{'coding_region_end'} = undef;
+ $self->{'cdna_coding_start'} = undef;
+ $self->{'cdna_coding_end'} = undef;
+}
- # attach the new list of exons to the transcript
- push @{$self->{'_trans_exon_array'}},@mapped_list_of_exons;
- if( defined $self->{'translation'} ) {
- $self->translation->transform( $href_exons );
- }
-}
-=head2 coding_start
+=head2 display_id
Arg [1] : none
- Example : none
- Description: DEPRECATED use coding_region_start instead
- Returntype : none
+ Example : print $transcript->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For transcripts this is the
+ stable id if it is available otherwise it is an empty string.
+ Returntype : string
Exceptions : none
- Caller : none
+ Caller : web drawing code
=cut
-sub coding_start {
- my ($self, @args) = @_;
+sub display_id {
+ my $self = shift;
+ return $self->{'stable_id'} || '';
+}
- $self->warn("coding_start has been renamed coding_region_start\n" .
- join(':'. caller));
- return $self->coding_region_start(@args);
-}
+###########################
+# DEPRECATED METHODS FOLLOW
+###########################
+# _translation_id
+# Usage : DEPRECATED - not needed anymore
-=head2 coding_end
- Arg [1] : none
- Example : none
- Description: DEPRECATED use coding_region_end instead
- Returntype : none
- Exceptions : none
- Caller : none
+
+=head2 sort
+
+ Description: DEPRECATED. This method is no longer needed. Exons are sorted
+ automatically when added to the transcript.
=cut
-sub coding_end {
- my ($self, @args) = @_;
+sub sort {
+ my $self = shift;
+
+ deprecate( "Exons are kept sorted, you dont have to call sort any more" );
+ # Fetch all the features
+ my @exons = @{$self->get_all_Exons()};
+
+ # Empty the feature table
+ $self->flush_Exons();
+
+ # Now sort the exons and put back in the feature table
+ my $strand = $exons[0]->strand;
- $self->warn("coding_end has been renamed coding_region_end\n" .
- join(':',caller));
+ if ($strand == 1) {
+ @exons = sort { $a->start <=> $b->start } @exons;
+ } elsif ($strand == -1) {
+ @exons = sort { $b->start <=> $a->start } @exons;
+ }
- return $self->coding_region_end(@args);
+ foreach my $e (@exons) {
+ $self->add_Exon($e);
+ }
}
+sub _translation_id {
+ my $self = shift;
+ deprecate( "This method shouldnt be necessary any more" );
+ if( @_ ) {
+ my $value = shift;
+ $self->{'_translation_id'} = $value;
+ }
+ return $self->{'_translation_id'};
-=head2 DEPRECATED add_DBLink
+}
- Arg [1] : DEPRECATED Bio::Annotation::DBLink $link
- a link is a database entry somewhere else.
- Usually this is a Bio::EnsEMBL::DBEntry.
- Example : DEPRECATED
- Description: This method has been deprecated in favor of the add_DBEntry
- method. Objects are responible for holding only xrefs directly
- associated with themselves now.
- Returntype : none
- Exceptions : none
- Caller : general
+=head2 created
+
+ Description: DEPRECATED - this attribute is not part of transcript anymore
=cut
+sub created{
+ my $obj = shift;
+ deprecate( "This attribute is no longer supported" );
+ if( @_ ) {
+ my $value = shift;
+ $obj->{'created'} = $value;
+ }
+ return $obj->{'created'};
+}
-sub add_DBLink{
- my ($self,$value) = @_;
- $self->throw("add_DBLink is deprecated. You probably want add_DBEntry.");
+=head2 modified
-# unless(defined $value && ref $value &&
-# $value->isa('Bio::Annotation::DBLink') ) {
-# $self->throw("This [$value] is not a DBLink");
-# }
+ Description: DEPRECATED - this attribute is not part of transcript anymore
-# if( !defined $self->{'_db_link'} ) {
-# $self->{'_db_link'} = [];
-# }
+=cut
+
+sub modified{
+ my $obj = shift;
+ deprecate( "This attribute is no longer supported" );
+ if( @_ ) {
+ my $value = shift;
+ $obj->{'modified'} = $value;
+ }
+ return $obj->{'modified'};
+}
+
+=head2 temporary_id
+
+ Function: DEPRECATED: Use dbID or stable_id or something else instead
+
+=cut
-# push(@{$self->{'_db_link'}},$value);
+sub temporary_id{
+ my ($obj,$value) = @_;
+ deprecate( "I cant see what a temporary_id is good for, please use dbID" .
+ "or stableID or\ntry without an id." );
+ if( defined $value) {
+ $obj->{'temporary_id'} = $value;
+ }
+ return $obj->{'temporary_id'};
}
+
1;
diff --git a/modules/Bio/EnsEMBL/TranscriptFactory.pm b/modules/Bio/EnsEMBL/TranscriptFactory.pm
index ed5af020f3..b260550ba9 100755
--- a/modules/Bio/EnsEMBL/TranscriptFactory.pm
+++ b/modules/Bio/EnsEMBL/TranscriptFactory.pm
@@ -68,9 +68,9 @@ sub fset2transcript {
$exon->strand ($f->strand);
$exon->phase ($f->phase);
$exon->end_phase( ($exon->phase + $exon->length)%3 );
- $exon->score($f->score);
+ #$exon->score($f->score);
$exon->p_value($f->p_value);
- $exon->attach_seq($contig->primary_seq);
+ $exon->slice($contig->primary_seq);
push(@exons,$exon);
$count++;
@@ -132,9 +132,9 @@ sub fset2transcript_guess_phases {
$exon->start ($f->start);
$exon->end ($f->end );
$exon->strand ($f->strand);
- $exon->score($f->score);
+ #$exon->score($f->score);
$exon->p_value($f->p_value);
- $exon->attach_seq($contig);
+ $exon->slice($contig);
$exon->phase($f->phase);
push(@exons,$exon);
$count++;
@@ -233,10 +233,10 @@ sub fset2transcript_3frame {
$exon->start ($f->start);
$exon->end ($f->end );
$exon->strand ($f->strand);
- $exon->attach_seq($contig);
+ $exon->slice($contig);
$exon->phase ($endphase);
$exon->end_phase( ($exon->phase + $exon->length)%3 );
- $exon->score ($f->score);
+ #$exon->score ($f->score);
$exon->p_value ($f->p_value);
$endphase = $exon->end_phase;
@@ -299,9 +299,9 @@ sub fset2transcript_with_seq {
$exon->strand ($f->strand);
$exon->phase ($f->phase);
$exon->end_phase( ($exon->phase + $exon->length)%3 );
- $exon->score ($f->score);
+ #$exon->score ($f->score);
#print STDERR "contig is a = ".$seq."\n";
- $exon->attach_seq($seq);
+ $exon->slice($seq);
push(@exons,$exon);
$count++;
diff --git a/modules/Bio/EnsEMBL/Translation.pm b/modules/Bio/EnsEMBL/Translation.pm
index e8cd6ccfbe..7af1a8b688 100755
--- a/modules/Bio/EnsEMBL/Translation.pm
+++ b/modules/Bio/EnsEMBL/Translation.pm
@@ -1,5 +1,5 @@
#
-# BioPerl module for Bio::EnsEMBL::Translation
+# EnsEMBL module for Bio::EnsEMBL::Translation
#
# Cared for by Ewan Birney <birney@sanger.ac.uk>
#
@@ -11,23 +11,23 @@
=head1 NAME
-Bio::EnsEMBL::Translation - DESCRIPTION of Object
+Bio::EnsEMBL::Translation - A class representing the translation of a
+transcript
=head1 SYNOPSIS
-Give standard usage here
+
=head1 DESCRIPTION
-Describe the object here
+A transcript''s translation defines the CDS and UTR regions of the transcript
+through the use of start_Exon/end_Exon, and start/end attributes.
=head1 CONTACT
-Describe contact details here
-
-=head1 APPENDIX
+Post questions to the EnsEMBL Developer list: ensembl-dev@ebi.ac.uk
-The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _
+=head1 METHODS
=cut
@@ -36,10 +36,12 @@ package Bio::EnsEMBL::Translation;
use vars qw($AUTOLOAD @ISA);
use strict;
+use Bio::EnsEMBL::Utils::Exception qw( deprecate throw warning );
+use Bio::EnsEMBL::Utils::Argument qw( rearrange );
-use Bio::EnsEMBL::Root;
+use Bio::EnsEMBL::Storable;
-@ISA = qw(Bio::EnsEMBL::Root);
+@ISA = qw(Bio::EnsEMBL::Storable);
sub new {
@@ -47,7 +49,23 @@ sub new {
my $class = ref($caller) || $caller;
- return bless {},$class;
+ my ( $start_exon, $end_exon, $seq_start, $seq_end,
+ $stable_id, $version, $dbID, $adaptor ) =
+ rearrange( [ "START_EXON", "END_EXON", "SEQ_START", "SEQ_END",
+ "STABLE_ID", "VERSION", "DBID", "ADAPTOR" ], @_ );
+
+ my $self = bless {
+ 'start_exon' => $start_exon,
+ 'end_exon' => $end_exon,
+ 'adaptor' => $adaptor,
+ 'dbID' => $dbID,
+ 'start' => $seq_start,
+ 'end' => $seq_end,
+ 'stable_id' => $stable_id,
+ 'version' => $version
+ }, $class;
+
+ return $self;
}
@@ -118,7 +136,7 @@ sub start_Exon {
if( @_ ) {
my $value = shift;
if( !ref $value || !$value->isa('Bio::EnsEMBL::Exon') ) {
- $self->throw("Got to have an Exon object, not a $value");
+ throw("Got to have an Exon object, not a $value");
}
$self->{'start_exon'} = $value;
}
@@ -146,7 +164,7 @@ sub end_Exon {
if( @_ ) {
my $value = shift;
if( !ref $value || !$value->isa('Bio::EnsEMBL::Exon') ) {
- $self->throw("Got to have an Exon object, not a $value");
+ throw("Got to have an Exon object, not a $value");
}
$self->{'end_exon'} = $value;
}
@@ -155,142 +173,40 @@ sub end_Exon {
}
-=head2 version
-
- Title : version
- Usage : $obj->version()
- Function:
- Returns : value of version
- Args :
-
-=cut
-
-sub version{
-
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_version'} = $value;
- }
-
- if( exists $self->{'_version'} ) {
- return $self->{'_version'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_version'};
-
-}
-
-
-
-=head2 stable_id
-
- Title : stable_id
- Usage : $obj->stable_id
- Function:
- Returns : value of stable_id
- Args :
-
-=cut
-
-sub stable_id{
-
- my ($self,$value) = @_;
-
-
- if( defined $value ) {
- $self->{'_stable_id'} = $value;
- return;
- }
-
- if( exists $self->{'_stable_id'} ) {
- return $self->{'_stable_id'};
- }
-
- $self->_get_stable_entry_info();
-
- return $self->{'_stable_id'};
-
-}
-
-
-sub _get_stable_entry_info {
- my $self = shift;
-
- if( !defined $self->adaptor ) {
- return undef;
- }
-
- $self->adaptor->get_stable_entry_info($self);
-
-}
-
-
-=head2 temporary_id
-
- Title : temporary_id
- Usage : $obj->temporary_id($newval)
- Function:
- Returns : value of temporary_id
- Args : newvalue (optional)
-
-=cut
-
-sub temporary_id {
- my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'tempID'} = $value;
- }
- return $self->{'tempID'};
-
-}
-
-
-
-=head2 dbID
+=head2 version
- Title : dbID
- Usage : $obj->dbID($newval)
- Function:
- Returns : value of dbID
- Args : newvalue (optional)
+ Arg [1] : string $version
+ Example : none
+ Description: get/set for attribute version
+ Returntype : string
+ Exceptions : none
+ Caller : general
=cut
-sub dbID {
+sub version {
my $self = shift;
- if( @_ ) {
- my $value = shift;
- $self->{'dbID'} = $value;
- }
- return $self->{'dbID'};
-
+ $self->{'version'} = shift if( @_ );
+ return $self->{'version'};
}
+=head2 stable_id
-=head2 adaptor
-
- Title : adaptor
- Usage : $obj->adaptor($newval)
- Function:
- Returns : value of adaptor
- Args : newvalue (optional)
+ Arg [1] : string $stable_id
+ Example : none
+ Description: get/set for attribute stable_id
+ Returntype : string
+ Exceptions : none
+ Caller : general
=cut
-sub adaptor {
+sub stable_id {
my $self = shift;
- if( @_ ) {
- $self->{'adaptor'} = shift;
- }
- return $self->{'adaptor'};
-
+ $self->{'stable_id'} = shift if( @_ );
+ return $self->{'stable_id'};
}
@@ -378,7 +294,7 @@ sub add_DBEntry {
my $dbe = shift;
unless($dbe && ref($dbe) && $dbe->isa('Bio::EnsEMBL::DBEntry')) {
- $self->throw('Expected DBEntry argument');
+ throw('Expected DBEntry argument');
}
$self->{'dbentries'} ||= [];
@@ -405,4 +321,140 @@ sub get_all_DBLinks {
}
+
+
+=head2 get_all_ProteinFeatures
+
+ Arg [1] : (optional) string $logic_name
+ The analysis logic_name of the features to retrieve. If not
+ specified, all features are retrieved instead.
+ Example : $features = $self->get_all_ProteinFeatures('PFam');
+ Description: Retrieves all ProteinFeatures associated with this
+ Translation. If a logic_name is specified, only features with
+ that logic_name are returned. If no logic_name is provided all
+ associated protein_features are returned.
+ Returntype : Bio::EnsEMBL::ProteinFeature
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub get_all_ProteinFeatures {
+ my $self = shift;
+ my $logic_name = shift;
+
+ if(!$self->{'protein_features'}) {
+ my $adaptor = $self->adaptor();
+ my $dbID = $self->dbID();
+ if(!$adaptor || !$dbID) {
+ warning("Cannot retrieve ProteinFeatures from translation without " .
+ "an attached adaptor and a dbID. Returning empty list.");
+ return [];
+ }
+
+ my %hash;
+ $self->{'protein_features'} = \%hash;
+
+ my $pfa = $adaptor->db()->get_ProteinFeatureAdaptor();
+ my $name;
+ foreach my $f (@{$pfa->fetch_all_by_translation_id($dbID)}) {
+ my $analysis = $f->analysis();
+ if($analysis) {
+ $name = lc($f->analysis->logic_name());
+ } else {
+ warning("ProteinFeature has no attached analysis\n");
+ $name = '';
+ }
+ $hash{$name} ||= [];
+ push @{$hash{$name}}, $f;
+ }
+ }
+
+ #a specific type of protein feature was requested
+ if(defined($logic_name)) {
+ $logic_name = lc($logic_name);
+ return $self->{'protein_features'}->{$logic_name} || [];
+ }
+
+ my @features;
+
+ #all protein features were requested
+ foreach my $type (keys %{$self->{'protein_features'}}) {
+ push @features, @{$self->{'protein_features'}->{$type}};
+ }
+
+ return \@features;
+}
+
+
+
+=head2 get_all_DomainFeatures
+
+ Arg [1] : none
+ Example : @domain_feats = @{$translation->get_all_DomainFeatures};
+ Description: A convenience method which retrieves all protein features
+ that are considered to be 'Domain' features. Features which
+ are 'domain' features are those with analysis logic names:
+ 'pfscan', 'scanprosite', 'superfamily', 'pfam', 'prints'.
+ Returntype : listref of Bio::EnsEMBL::ProteinFeatures
+ Exceptions : none
+ Caller : webcode (protview)
+
+=cut
+
+sub get_all_DomainFeatures{
+ my ($self) = @_;
+
+ my @features;
+
+ my @types = ('pfscan', #profile (prosite or pfam motifs)
+ 'scanprosite', #prosite
+ 'superfamily',
+ 'pfam',
+ 'prints');
+
+ foreach my $type (@types) {
+ push @features, @{$self->get_all_ProteinFeatures($type)};
+ }
+
+ return \@features;
+}
+
+=head2 display_id
+
+ Arg [1] : none
+ Example : print $translation->display_id();
+ Description: This method returns a string that is considered to be
+ the 'display' identifier. For translations this is the
+ stable id if it is available otherwise it is an empty string.
+ Returntype : string
+ Exceptions : none
+ Caller : web drawing code
+
+=cut
+
+sub display_id {
+ my $self = shift;
+ return $self->{'stable_id'} || '';
+}
+
+
+
+
+=head2 temporary_id
+
+ Description: DEPRECATED This method should not be needed. Use dbID,
+ stable_id or something else.
+
+=cut
+
+sub temporary_id {
+ my $self = shift;
+ deprecate( "I cant see what a temporary_id is good for, please use " .
+ "dbID or stableID or\n try without an id." );
+ $self->{'temporary_id'} = shift if( @_ );
+ return $self->{'temporary_id'};
+}
+
+
1;
diff --git a/modules/Bio/EnsEMBL/Utils/Argument.pm b/modules/Bio/EnsEMBL/Utils/Argument.pm
new file mode 100644
index 0000000000..ff3b5591f7
--- /dev/null
+++ b/modules/Bio/EnsEMBL/Utils/Argument.pm
@@ -0,0 +1,162 @@
+# EnsEMBL module for Bio::EnsEMBL::Utils::Exception
+#
+#
+
+=head1 NAME
+
+Bio::EnsEMBL::Utils::Argument - Utility functions for argument handling
+
+=head1 SYNOPSIS
+
+ use Bio::EnsEMBL::Utils::Argument qw(rearrange)
+
+ package Feature;
+
+ sub new {
+ my $class = shift;
+ my ($start, $end, $strand) = rearrange(['START', 'END', 'STRAND'],@_);
+ return bless({'start'=>$start, 'end'=>$end, 'strand'=>$strand}, $class);
+ }
+
+=head1 DESCRIPTION
+
+This is derived from the Bio::Root module in BioPerl. The _rearrange
+object method taken from BioPerl has been renamed rearrange and is now a
+static class method. This method was originally written by Lincoln Stein,
+and has since been refactored several times by various people (as described
+below).
+
+It is recommended that this package be used instead of inheriting
+unnecessarily from the Bio::EnsEMBL::Root or Bio::Root object.
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL development list: ensembl-dev@ebi.ac.uk
+
+=head1 APPENDIX
+
+The rest of the documentation details exported static class methods.
+
+=cut
+
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::Utils::Argument;
+
+use Exporter;
+
+use vars qw(@ISA @EXPORT_OK);
+
+@ISA = qw(Exporter);
+
+@EXPORT_OK = qw(&rearrange);
+
+
+
+=head2 rearrange
+
+ Usage : rearrange( array_ref, list_of_arguments)
+ Purpose : Rearranges named parameters to requested order.
+ Example : use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+ : rearrange([qw(SEQUENCE ID DESC)],@param);
+ : Where @param = (-sequence => $s,
+ : -id => $i,
+ : -desc => $d);
+ Returns : @params - an array of parameters in the requested order.
+ : The above example would return ($s, $i, $d)
+ Argument : $order : a reference to an array which describes the desired
+ : order of the named parameters.
+ : @param : an array of parameters, either as a list (in
+ : which case the function simply returns the list),
+ : or as an associative array with hyphenated tags
+ : (in which case the function sorts the values
+ : according to @{$order} and returns that new array.)
+ : The tags can be upper, lower, or mixed case
+ : but they must start with a hyphen (at least the
+ : first one should be hyphenated.)
+ Source : This function was taken from CGI.pm, written by Dr. Lincoln
+ : Stein, and adapted for use in Bio::Seq by Richard Resnick and
+ : then adapted for use in Bio::Root::Object.pm by Steve A. Chervitz.
+ : This has since been adapted as an exported static method in this
+ class Bio::EnsEMBL::Utils::Argument
+ Comments : (SAC)
+ : This method may not be appropriate for method calls that are
+ : within in an inner loop if efficiency is a concern.
+ :
+ : Parameters can be specified using any of these formats:
+ : @param = (-name=>'me', -color=>'blue');
+ : @param = (-NAME=>'me', -COLOR=>'blue');
+ : @param = (-Name=>'me', -Color=>'blue');
+ : @param = ('me', 'blue');
+ : A leading hyphenated argument is used by this function to
+ : indicate that named parameters are being used.
+ : Therefore, the ('me', 'blue') list will be returned as-is.
+ :
+ : Note that Perl will confuse unquoted, hyphenated tags as
+ : function calls if there is a function of the same name
+ : in the current namespace:
+ : -name => 'foo' is interpreted as -&name => 'foo'
+ :
+ : For ultimate safety, put single quotes around the tag:
+ : ('-name'=>'me', '-color' =>'blue');
+ : This can be a bit cumbersome and I find not as readable
+ : as using all uppercase, which is also fairly safe:
+ : (-NAME=>'me', -COLOR =>'blue');
+ :
+ : Personal note (SAC): I have found all uppercase tags to
+ : be more managable: it involves less single-quoting,
+ : the code is more readable, and there are no method naming
+ : conlicts.
+ : Regardless of the style, it greatly helps to line
+ : the parameters up vertically for long/complex lists.
+
+=cut
+
+
+sub rearrange {
+ my($order,@param) = @_;
+
+ return unless @param;
+
+ # If we've got parameters, we need to check to see whether
+ # they are named or simply listed. If they are listed, we
+ # can just return them.
+
+ return @param unless (defined($param[0]) && $param[0]=~/^-/);
+
+ # Now we've got to do some work on the named parameters.
+ # The next few lines strip out the '-' characters which
+ # preceed the keys, and capitalizes them.
+ my $i;
+ for ($i=0;$i<@param;$i+=2) {
+ $param[$i]=~s/^\-//;
+ $param[$i]=~tr/a-z/A-Z/;
+ }
+
+ # Now we'll convert the @params variable into an associative array.
+ local($^W) = 0; # prevent "odd number of elements" warning with -w.
+ my(%param) = @param;
+
+ my(@return_array);
+
+ # What we intend to do is loop through the @{$order} variable,
+ # and for each value, we use that as a key into our associative
+ # array, pushing the value at that key onto our return array.
+ my($key);
+
+ foreach $key (@{$order}) {
+ $key = uc($key);
+ my($value) = $param{$key};
+ delete $param{$key};
+ push(@return_array,$value);
+ }
+
+ return (@return_array);
+}
+
+
+1;
+
+
diff --git a/modules/Bio/EnsEMBL/Utils/Converter.pm b/modules/Bio/EnsEMBL/Utils/Converter.pm
index 6ac9fe96f0..e17464b039 100644
--- a/modules/Bio/EnsEMBL/Utils/Converter.pm
+++ b/modules/Bio/EnsEMBL/Utils/Converter.pm
@@ -161,7 +161,7 @@ sub convert{
return \@output;
}
-sub _convert_single{
+sub _converter_single{
my ($self, $input) = @_;
$self->throw("Not implemented. Please check the instance subclass");
}
diff --git a/modules/Bio/EnsEMBL/Utils/Exception.pm b/modules/Bio/EnsEMBL/Utils/Exception.pm
new file mode 100644
index 0000000000..596e012dec
--- /dev/null
+++ b/modules/Bio/EnsEMBL/Utils/Exception.pm
@@ -0,0 +1,424 @@
+# EnsEMBL module for Bio::EnsEMBL::Utils::Exception
+#
+#
+
+=head1 NAME
+
+Bio::EnsEMBL::Utils::Exception - Utility functions for error handling
+
+=head1 SYNOPSIS
+
+ use Bio::EnsEMBL::Utils::Exception qw(throw warning deprecate verbose)
+
+ eval {
+ throw("this is an exception with a stack trace");
+ );
+
+ if($@) {
+ print "Caught exception:\n$@";
+ }
+
+ #silence warnings
+ verbose('OFF');
+
+ warning('this is a silent warning');
+
+ #show deprecated and warning messages but not info
+ verbose('DEPRECATE');
+
+ warning('this is a warning');
+
+ #show all messages
+ verbose('ALL');
+
+ info('this is an informational message');
+
+ sub my_sub {
+ deprecate('use other_sub() instead');
+ }
+
+ verbose('EXCEPTION');
+ info('This is a high priority info message.', 1000);
+
+
+=head1 DESCRIPTION
+
+This is derived from the Bio::Root module in BioPerl. Some formatting has
+been changed and the deprecate function has been added. Most notably the
+object methods are now static class methods that can be called without
+inheriting from Bio::Root or Bio::EnsEMBL::Root. This is especially useful
+for throwing exceptions with stack traces outside of a blessed context.
+
+The originaly implementations of these methods were by Steve Chervitz and
+refactored by Ewan Birney.
+
+It is recommended that these functions be used instead of inheriting
+unnecessarily from the Bio::EnsEMBL::Root or Bio::Root object.
+The functions exported by this package provide a set of useful error handling
+methods.
+
+=head1 CONTACT
+
+Post questions to the EnsEMBL development list: ensembl-dev@ebi.ac.uk
+
+=head1 METHODS
+
+The rest of the documentation details exported static class methods.
+
+=cut
+
+use strict;
+use warnings;
+
+package Bio::EnsEMBL::Utils::Exception;
+
+use Exporter;
+
+use vars qw(@ISA @EXPORT_OK);
+
+@ISA = qw(Exporter);
+
+@EXPORT_OK = qw(&throw &warning &stack_trace_dump
+ &stack_trace &verbose &deprecate &info);
+
+my $VERBOSITY = 3000;
+my $DEFAULT_INFO = 4000;
+my $DEFAULT_DEPRECATE = 3000;
+my $DEFAULT_WARNING = 2000;
+my $DEFAULT_EXCEPTION = 1000;
+
+
+=head2 throw
+
+ Arg [1] : string $msg
+ Arg [2] : (optional) int $level
+ override the default level of exception throwing
+ Example : use Bio::EnsEMBL::Utils::Exception qw(throw);
+ throw('We have a problem');
+ Description: Throws an exception which if not caught by an eval will
+ provide a stack trace to STDERR and die. If the verbosity level
+ is lower than the level of the throw, then no error message is
+ displayed but the program will still die (unless the exception
+ is caught).
+ Returntype : none
+ Exceptions : thrown every time
+ Caller : generally on error
+
+=cut
+
+sub throw {
+ my $string = shift;
+
+ #for backwards compatibility with Bio::EnsEMBL::Root::throw
+ #allow to be called as an object method as well as class method
+ $string = shift if(ref($string)); #skip object if one provided
+
+ my $level = shift;
+
+ $level = $DEFAULT_EXCEPTION if(!defined($level));
+
+ if($VERBOSITY < $level) {
+ die("\n"); #still die, but silently
+ }
+
+ my $std = stack_trace_dump(3);
+
+ my $out = "\n-------------------- EXCEPTION --------------------\n" .
+ "MSG: $string\n" .
+ "$std" .
+ "---------------------------------------------------\n";
+ die $out;
+}
+
+
+
+=head2 warning
+
+ Arg [1] : string warning(message);
+ Arg [2] : (optional) int level
+ Override the default level of this warning changning the level
+ of verbosity at which it is displayed.
+ Example : use Bio::EnsEMBL::Utils::Exception qw(warning)
+ warning('This is a warning');
+ Description: If the verbosity level is higher or equal to the level of this
+ warning then a warning message is printed to STDERR. If the
+ verbosity lower then nothing is done. Under the default
+ levels of warning and verbosity warnings will be displayed.
+ Returntype : none
+ Exceptions : warning every time
+ Caller : general
+
+=cut
+
+sub warning {
+ my $string = shift;
+ my $level = shift;
+
+ $level = $DEFAULT_WARNING if(!defined($level));
+
+ return if ($VERBOSITY < $level);
+
+ my @caller = caller;
+ my $line = $caller[2] || '';
+
+ #use only 2 subdirs for brevity when reporting the filename
+ my $file;
+ my @path = split(/\//, $caller[1]);
+ $file = pop(@path);
+ my $i = 0;
+ while(@path && $i < 2) {
+ $i++;
+ $file = pop(@path) ."/$file";
+ }
+
+ my $out = "\n-------------------- WARNING ----------------------\n".
+ "MSG: $string\n".
+ "FILE: $file LINE: $line\n" .
+ "---------------------------------------------------\n";
+ print STDERR $out;
+}
+
+
+
+=head2 info
+
+ Arg [1] : string $string
+ The message to be displayed
+ Arg [2] : (optional) int $level
+ Override the default level of this message so it is displayed at
+ a different level of verbosity than it normally would be.
+ Example : use Bio::EnsEMBL::Utils::Exception qw(verbose info)
+ Description: This prints an info message to STDERR if verbosity is higher
+ than the level of the message. By default info messages are not
+ displayed.
+ Returntype : none
+ Exceptions : none
+ Caller : general
+
+=cut
+
+sub info {
+ my $string = shift;
+ my $level = shift;
+
+ $level = $DEFAULT_INFO if(!defined($level));
+
+ return if($VERBOSITY < $level);
+
+ print STDERR "INFO: $string\n";
+}
+
+
+
+=head2 verbose
+
+ Arg [1] : (optional) int
+ Example : use Bio::EnsEMBL::Utils::Exception qw(verbose warning);
+ #turn warnings and everything more important on (e.g. exception)
+ verbose('WARNING');
+ warning("Warning displayed");
+ info("This won't be displayed");
+ deprecate("This won't be diplayed");
+
+ #turn exception messages on
+ verbose('EXCEPTION');
+ warning("This won't do anything");
+ throw("Die with a message");
+
+ #turn everying off
+ verbose('OFF'); #same as verbose(0);
+ warning("This won't do anything");
+ throw("Die silently without a message");
+
+ #turn on all messages
+ verbose('ALL');
+ info("All messages are now displayed");
+
+ if(verbose() > 3000) {
+ print "Verbosity is pretty high";
+ }
+
+ Description: Gets/Sets verbosity level which defines which messages are
+ to be displayed. An integer value may be passed or one of the
+ following strings:
+ 'OFF' (= 0)
+ 'EXCEPTION' (= 1000)
+ 'WARNING' (= 2000)
+ 'DEPRECATE' (= 3000)
+ 'INFO' (= 4000)
+ 'ALL' (= 1000000)
+
+ Returntype : int
+ Exceptions : none
+ Caller : general
+
+=cut
+
+
+sub verbose {
+ if(@_) {
+ my $verbosity = shift;
+ if($verbosity =~ /\d+/) { #check if verbosity is an integer
+ $VERBOSITY = $verbosity;
+ } else {
+ $verbosity = uc($verbosity);
+ if($verbosity eq 'OFF' || $verbosity eq 'NOTHING' ||
+ $verbosity eq 'NONE') {
+ $VERBOSITY = 0;
+ } elsif($verbosity eq 'EXCEPTION' || $verbosity eq 'THROW') {
+ $VERBOSITY = $DEFAULT_EXCEPTION;
+ } elsif($verbosity eq 'WARNING' || $verbosity eq 'WARN') {
+ $VERBOSITY = $DEFAULT_WARNING;
+ } elsif($verbosity eq 'DEPRECATE' || $verbosity eq 'DEPRECATED') {
+ $VERBOSITY = $DEFAULT_DEPRECATE;
+ } elsif($verbosity eq 'INFO') {
+ $VERBOSITY = $DEFAULT_INFO;
+ } elsif($verbosity eq 'ON' || $verbosity eq 'ALL') {
+ $VERBOSITY = 1e6;
+ } else {
+ $VERBOSITY = $DEFAULT_WARNING;
+ warning("Unknown level of verbosity: $verbosity");
+ }
+ }
+ }
+
+ return $VERBOSITY;
+}
+
+
+
+=head2 stack_trace_dump
+
+ Arg [1] : (optional) int $levels
+ The number of levels to ignore from the top of the stack when
+ creating the dump. This is useful when this is called internally
+ from a warning or throw function when the immediate caller and
+ stack_trace_dump function calls are themselves uninteresting.
+ Example : use Bio::EnsEMBL::Utils::Exception qw(stack_trace_dump);
+ print STDERR stack_trace_dump();
+ Description: Returns a stack trace formatted as a string
+ Returntype : string
+ Exceptions : none
+ Caller : general, throw, warning
+
+=cut
+
+sub stack_trace_dump{
+ my @stack = stack_trace();
+
+ my $levels = 2; #default is 2 levels so stack_trace_dump call is not present
+ $levels = shift if(@_);
+ $levels = 1 if($levels < 1);
+
+ while($levels) {
+ $levels--;
+ shift @stack;
+ }
+
+ my $out;
+ my ($module,$function,$file,$position);
+
+
+ foreach my $stack ( @stack) {
+ ($module,$file,$position,$function) = @{$stack};
+ $out .= "STACK $function $file:$position\n";
+ }
+
+ return $out;
+}
+
+
+
+=head2 stack_trace
+
+ Arg [1] : none
+ Example : use Bio::EnsEMBL::Utils::Exception qw(stack_trace)
+ Description: Gives an array to a reference of arrays with stack trace info
+ each coming from the caller(stack_number) call
+ Returntype : array of listrefs of strings
+ Exceptions : none
+ Caller : general, stack_trace_dump()
+
+=cut
+
+sub stack_trace {
+ my $i = 0;
+ my @out;
+ my $prev;
+ while ( my @call = caller($i++)) {
+
+ # major annoyance that caller puts caller context as
+ # function name. Hence some monkeying around...
+ $prev->[3] = $call[3];
+ push(@out,$prev);
+ $prev = \@call;
+ }
+ $prev->[3] = 'toplevel';
+ push(@out,$prev);
+ return @out;
+}
+
+
+=head2 deprecate
+
+ Arg [1] : string $mesg
+ A message describing why a method is deprecated
+ Example : use Bio::EnsEMBL::Utils::Exception qw(deprecate)
+ sub old_sub {
+ deprecate('Please use new_sub() instead');
+ }
+ Description: Prints a warning to STDERR that the method which called
+ deprecate() is deprecated. Also prints the line number and
+ file from which the deprecated method was called. Deprecated
+ warnings only appear once for each location the method was
+ called from. No message is displayed if the level of verbosity
+ is lower than the level of the warning.
+ Returntype : none
+ Exceptions : warning every time
+ Caller : deprecated methods
+
+=cut
+
+my %DEPRECATED;
+
+sub deprecate {
+ my $mesg = shift;
+
+ my $level = shift;
+
+ $level = $DEFAULT_DEPRECATE if(!defined($level));
+
+ return if($VERBOSITY < $level);
+
+ my @caller = caller(1);
+ my $subname = $caller[3] ;
+ my $line = $caller[2];
+
+ #use only 2 subdirs for brevity when reporting the filename
+ my $file;
+ my @path = $caller[1];
+ $file = pop(@path);
+ my $i = 0;
+ while(@path && $i < 2) {
+ $i++;
+ $file .= pop(@path);
+ }
+
+ #keep track of who called this method so that the warning is only displayed
+ #once per deprecated call
+ return if $DEPRECATED{"$line:$file"};
+
+ if($VERBOSITY > -1) {
+ print STDERR "\n------------------ DEPRECATED ---------------------\n" .
+ "Deprecated method call in file $file line $line.\n" .
+ "Method $subname is deprecated.\n" .
+ "$mesg\n" .
+ "---------------------------------------------------\n";
+ }
+
+ $DEPRECATED{"$line:$file"} = 1;
+}
+
+
+
+1;
diff --git a/modules/Bio/EnsEMBL/Utils/SeqDumper.pm b/modules/Bio/EnsEMBL/Utils/SeqDumper.pm
index 1c096b3c47..40abb3399c 100644
--- a/modules/Bio/EnsEMBL/Utils/SeqDumper.pm
+++ b/modules/Bio/EnsEMBL/Utils/SeqDumper.pm
@@ -36,7 +36,7 @@ package Bio::EnsEMBL::Utils::SeqDumper;
use IO::File;
use vars qw(@ISA);
-@ISA = qw(Bio::EnsEMBL::Root);
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
#keys must be uppercase
my $DUMP_HANDLERS =
@@ -112,12 +112,12 @@ sub new {
sub enable_feature_type {
my ($self, $type) = @_;
- $type || $self->throw("type arg is required");
+ $type || throw("type arg is required");
if(exists($self->{'feature_types'}->{$type})) {
$self->{'feature_types'}->{$type} = 1;
} else {
- $self->warn("unknown feature type '$type'\n" .
+ warning("unknown feature type '$type'\n" .
"valid types are: " . join(',', keys %{$self->{'feature_types'}}));
}
}
@@ -141,10 +141,9 @@ sub enable_feature_type {
sub attach_database {
my ($self, $name, $db) = @_;
- $name || $self->throw("name arg is required");
+ $name || throw("name arg is required");
unless($db && ref($db) && $db->isa('Bio::EnsEMBL::DBSQL::DBConnection')) {
- $self->throw("db arg must be a Bio::EnsEMBL::DBSQL::DBConnection not a " .
- "[$db]");
+ throw("db arg must be a Bio::EnsEMBL::DBSQL::DBConnection not a [$db]");
}
$self->{'attached_dbs'}->{$name} = $db;
@@ -167,7 +166,7 @@ sub attach_database {
sub get_database {
my ($self, $name) = @_;
- $name || $self->throw("name arg is required");
+ $name || throw("name arg is required");
return $self->{'attached_dbs'}->{$name};
}
@@ -190,7 +189,7 @@ sub get_database {
sub remove_database {
my ($self, $name) = @_;
- $name || $self->throw("name arg is required");
+ $name || throw("name arg is required");
if(exists $self->{'attached_dbs'}->{$name}) {
return delete $self->{'attached_dbs'}->{$name};
@@ -215,12 +214,12 @@ sub remove_database {
sub disable_feature_type {
my ($self, $type) = @_;
- $type || $self->throw("type arg is required");
+ $type || throw("type arg is required");
if(exists($self->{'feature_types'}->{$type})) {
$self->{'feature_types'}->{$type} = 0;
} else {
- $self->warn("unknown feature type '$type'\n" .
+ warning("unknown feature type '$type'\n" .
"valid types are: " . join(',', keys %{$self->{'feature_types'}}));
}
}
@@ -242,12 +241,12 @@ sub disable_feature_type {
sub is_enabled {
my ($self, $type) = @_;
- $type || $self->throw("type arg is required");
+ $type || throw("type arg is required");
if(exists($self->{'feature_types'}->{$type})) {
return $self->{'feature_types'}->{$type};
} else {
- $self->warn("unknown feature type '$type'\n" .
+ warning("unknown feature type '$type'\n" .
"valid types are: " . join(',', keys %{$self->{'feature_types'}}));
}
}
@@ -275,24 +274,24 @@ sub is_enabled {
sub dump {
my ($self, $slice, $format, $outfile) = @_;
- $format || $self->throw("format arg is required");
- $slice || $self->throw("slice arg is required");
+ $format || throw("format arg is required");
+ $slice || throw("slice arg is required");
my $dump_handler = $DUMP_HANDLERS->{uc($format)};
unless($dump_handler) {
- $self->throw("No dump handler is defined for format $format\n");
+ throw("No dump handler is defined for format $format\n");
}
my $FH = IO::File->new;;
if($outfile) {
- $FH->open(">$outfile") or $self->throw("Could not open file $outfile");
+ $FH->open(">$outfile") or throw("Could not open file $outfile");
} else {
$FH = \*STDOUT;
#mod_perl did not like the following
#$FH->fdopen(fileno(STDOUT), "w")
- # or $self->throw("Could not open currently selected output filehandle " .
+ # or throw("Could not open currently selected output filehandle " .
# "for writing");
}
@@ -321,9 +320,46 @@ sub dump_embl {
my $slice = shift;
my $FH = shift;
- my $id = $slice->name;
my $len = $slice->length;
+ my $version;
+ my $id;
+
+ my $cs = $slice->coord_system();
+ my $name_str = $cs->name() . ' ' . $slice->seq_region_name();
+ $name_str .= ' ' . $cs->version if($cs->version);
+
+ my $start = $slice->start;
+ my $end = $slice->end;
+
+ #determine if this slice is the entire seq region
+ #if it is then we just use the name as the id
+ my $slice_adaptor = $slice->adaptor();
+ my $full_slice =
+ $slice->adaptor->fetch_by_region($cs->name,
+ $slice->seq_region_name,
+ undef,undef,undef,
+ $cs->version);
+
+
+ if($full_slice->name eq $slice->name) {
+ $name_str .= ' full sequence';
+ $id = $slice->seq_region_name();
+ my @acc_ver = split(/\./, $id);
+ if(@acc_ver == 2) {
+ $id = $acc_ver[0];
+ $version = $acc_ver[0] . $acc_ver[1];
+ } elsif(@acc_ver == 1 && $cs->version()) {
+ $version = $id . $cs->version();
+ } else {
+ $version = $id;
+ }
+ } else {
+ $name_str .= ' partial sequence';
+ $id = $slice->name();
+ $version = $id;
+ }
+
#line breaks are allowed near the end of the line on ' ', "\t", "\n", ','
$: = (" \t\n-,");
@@ -335,27 +371,29 @@ sub dump_embl {
my $EMBL_HEADER =
'@< ^<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<~
';
-
+
#ID and moltype
my $VALUE = "$id ENSEMBL; DNA; PLN; $len BP.";
$self->write($FH, $EMBL_HEADER, 'ID', $VALUE);
print $FH "XX\n";
-
+
#Accession
$self->write($FH, $EMBL_HEADER, 'AC', $id);
print $FH "XX\n";
-
+
#Version
- $self->write($FH, $EMBL_HEADER, 'SV', "$id.ENSEMBL_DB:".
- $slice->adaptor->db->dbname);
+ $self->write($FH, $EMBL_HEADER, 'SV', $version);
print $FH "XX\n";
#Date
$self->write($FH, $EMBL_HEADER, 'DT', $self->_date_string);
print $FH "XX\n";
+ my $species = $slice->adaptor->db->get_MetaContainer->get_Species();
+
#Description
- $self->write($FH, $EMBL_HEADER, 'DE', "Reannotated sequence via EnsEMBL");
+ $self->write($FH, $EMBL_HEADER, 'DE', $species->binomial .
+ " $name_str $start..$end reannotated via EnsEMBL");
print $FH "XX\n";
#key words
@@ -363,7 +401,6 @@ sub dump_embl {
print $FH "XX\n";
#Species
- my $species = $slice->adaptor->db->get_MetaContainer->get_Species();
my $species_name = $species->binomial();
if(my $cn = $species->common_name()) {
$species_name .= " ($cn)";
@@ -457,42 +494,67 @@ sub dump_genbank {
' ^<<<<<<<<<<<<<< ^<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<<~~
';
- my $id = $slice->name;
- my $length = $slice->length;
+ my $version;
+ my $id;
- my ($name_str, $start, $end);
- if($slice->isa('Bio::EnsEMBL::Slice')) {
- $name_str = "chromosome " . $slice->chr_name;
- $start = $slice->chr_start;
- $end = $slice->chr_end;
+ my $cs = $slice->coord_system();
+
+ my $name_str = $cs->name() . ' ' . $slice->seq_region_name();
+
+ $name_str .= ' ' . $cs->version if($cs->version);
+
+ #determine if this slice is the entire seq region
+ #if it is then we just use the name as the id
+ my $slice_adaptor = $slice->adaptor();
+ my $full_slice =
+ $slice->adaptor->fetch_by_region($cs->name,
+ $slice->seq_region_name,
+ undef,undef,undef,
+ $cs->version);
+
+
+ if($full_slice->name eq $slice->name) {
+ $name_str .= ' full sequence';
+ $id = $slice->seq_region_name();
+ my @acc_ver = split(/\./, $id);
+ if(@acc_ver == 2) {
+ $id = $acc_ver[0];
+ $version = $acc_ver[0] . $acc_ver[1];
+ } elsif(@acc_ver == 1 && $cs->version()) {
+ $version = $id . $cs->version();
+ } else {
+ $version = $id;
+ }
} else {
- $name_str = $slice->name;
- $start = 1;
- $end = $slice->length();
+ $name_str .= ' partial sequence';
+ $id = $slice->name();
+ $version = $id;
}
+ my $length = $slice->length;
+ my $start = $slice->start();
+ my $end = $slice->end();
+
my $date = $self->_date_string;
my $species = $slice->adaptor->db->get_MetaContainer->get_Species;
#LOCUS
my $tag = 'LOCUS';
- my $value = "ENS:$id $length bp DNA HTG $date";
+ my $value = "$id $length bp DNA HTG $date";
$self->write($FH, $GENBANK_HEADER, $tag, $value);
-
#DEFINITION
$tag = "DEFINITION";
- $value = $species->binomial . ' ' . $slice->adaptor->db->assembly_type .
- " assembly reannotated via EnsEMBL DNA, $name_str $start..$end";
+ $value = $species->binomial .
+ " $name_str $start..$end reannotated via EnsEMBL";
$self->write($FH, $GENBANK_HEADER, $tag, $value);
#ACCESSION
$self->write($FH, $GENBANK_HEADER, 'ACCESSION', $id);
#VERSION
- $self->write($FH, $GENBANK_HEADER, 'VERSION', "$id.ENSEMBL_DB:".
- $slice->adaptor->db->dbname);
+ $self->write($FH, $GENBANK_HEADER, 'VERSION', $version);
# KEYWORDS
$self->write($FH, $GENBANK_HEADER, 'KEYWORDS', '.');
@@ -512,15 +574,13 @@ sub dump_genbank {
foreach my $comment (@COMMENTS) {
$self->write($FH, $GENBANK_HEADER, 'COMMENT', $comment);
}
-
-
+
####################
# DUMP FEATURE TABLE
####################
print $FH "FEATURES Location/Qualifiers\n";
$self->_dump_feature_table($slice, $FH, $GENBANK_FT);
-
####################
# DUMP SEQUENCE
####################
@@ -594,33 +654,32 @@ sub _dump_feature_table {
if($self->is_enabled('gene')) {
push @gene_slices, $slice;
}
-
+
# Retrieve slices of other database where we need to pull genes from
-
+
my $gene_dbs = {'vegagene' => 'vega',
- 'estgene' => 'estgene'};
-
+ 'estgene' => 'estgene'};
+
foreach my $gene_type (keys %$gene_dbs) {
if($self->is_enabled($gene_type)) {
- my $db = $self->get_database($gene_dbs->{$gene_type});
- if($db) {
- warn "adding gene slice for db $gene_dbs->{$gene_type}";
- push @gene_slices, $db->get_SliceAdaptor->fetch_by_chr_start_end
- ($slice->chr_name, $slice->chr_start, $slice->chr_end);
- } else {
- $self->warn("A [". $gene_dbs->{$gene_type} ."] database must be " .
- "attached to this SeqDumper\n(via a call to " .
- "attach_database) to retrieve genes of type [$gene_type]");
- }
+ my $db = $self->get_database($gene_dbs->{$gene_type});
+ if($db) {
+ push @gene_slices, $db->get_SliceAdaptor->fetch_by_chr_start_end
+ ($slice->chr_name, $slice->chr_start, $slice->chr_end);
+ } else {
+ warning("A [". $gene_dbs->{$gene_type} ."] database must be " .
+ "attached to this SeqDumper\n(via a call to " .
+ "attach_database) to retrieve genes of type [$gene_type]");
+ }
}
}
}
-
+
foreach my $gene_slice (@gene_slices) {
foreach my $gene (@{$gene_slice->get_all_Genes}) {
foreach my $transcript (@{$gene->get_all_Transcripts}) {
my $translation = $transcript->translation;
-
+
# normal transcripts get dumped differently than pseudogenes
if($translation) {
#normal transcript
@@ -638,7 +697,7 @@ sub _dump_feature_table {
$self->write(@ff,'', '/protein_id="'.$translation->stable_id().'"');
$self->write(@ff,''
,'/note="transcript_id='.$transcript->stable_id().'"');
-
+
foreach my $dbl (@{$transcript->get_all_DBLinks}) {
$value = '/db_xref="'.$dbl->dbname().':'.$dbl->display_id().'"';
$self->write(@ff, '', $value);
@@ -661,13 +720,12 @@ sub _dump_feature_table {
}
}
}
-
+
# exons
foreach my $gene (@{$gene_slice->get_all_Genes}) {
foreach my $exon (@{$gene->get_all_Exons}) {
- $self->write(@ff,'exon', $self->features2location([$exon]));
- $self->write(@ff,'' , '/note="exon_id='.$exon->stable_id().'"');
-
+ $self->write(@ff,'exon', $self->features2location([$exon]));
+ $self->write(@ff,'' , '/note="exon_id='.$exon->stable_id().'"');
}
}
}
@@ -682,8 +740,9 @@ sub _dump_feature_table {
push @genscan_exons, @$exons;
$self->write(@ff, 'mRNA', $self->features2location($exons));
$self->write(@ff, '', '/product="'.$transcript->translate()->seq().'"');
+ $self->write(@ff, '', '/note="identifier='.$transcript->stable_id.'"');
$self->write(@ff, '', '/note="Derived by automated computational' .
- ' analysis using gene prediction method:' .
+ ' analysis using gene prediction method:' .
$transcript->analysis->logic_name . '"');
}
}
@@ -704,9 +763,9 @@ sub _dump_feature_table {
$self->write(@ff, '' , '/replace="'.$snp->alleles.'"');
#$self->write(@ff, '' , '/evidence="'.$snp->status.'"');
foreach my $link ($snp->each_DBLink) {
- my $id = $link->primary_id;
- my $db = $link->database;
- $self->write(@ff, '', "/db_xref=\"$db:$id\"");
+ my $id = $link->primary_id;
+ my $db = $link->database;
+ $self->write(@ff, '', "/db_xref=\"$db:$id\"");
}
}
@@ -715,7 +774,7 @@ sub _dump_feature_table {
#
# similarity features
- #
+ #
if($self->is_enabled('similarity')) {
foreach my $sim (@{$slice->get_all_SimilarityFeatures}) {
$self->write(@ff, 'misc_feature', $self->features2location([$sim]));
@@ -742,16 +801,18 @@ sub _dump_feature_table {
if($self->is_enabled('marker') && $slice->can('get_all_MarkerFeatures')) {
foreach my $mf (@{$slice->get_all_MarkerFeatures}) {
$self->write(@ff, 'STS', $self->features2location([$mf]));
- $self->write(@ff, '' , '/standard_name="' .
- $mf->marker->display_MarkerSynonym->name . '"');
+ if($mf->marker->display_MarkerSynonym) {
+ $self->write(@ff, '' , '/standard_name="' .
+ $mf->marker->display_MarkerSynonym->name . '"');
+ }
#grep out synonyms without a source
my @synonyms = @{$mf->marker->get_all_MarkerSynonyms};
@synonyms = grep {$_->source } @synonyms;
foreach my $synonym (@synonyms) {
- $self->write(@ff, '', '/db_xref="'.$synonym->source.
- ':'.$synonym->name.'"');
+ $self->write(@ff, '', '/db_xref="'.$synonym->source.
+ ':'.$synonym->name.'"');
}
$self->write(@ff, '', '/note="map_weight='.$mf->map_weight.'"');
}
@@ -760,13 +821,14 @@ sub _dump_feature_table {
#
# contigs
#
- if($self->is_enabled('contig') && $slice->can('get_tiling_path')) {
- foreach my $tile (@{$slice->get_tiling_path}) {
- $self->write(@ff, 'misc_feature',
- $tile->assembled_start .'..'. $tile->assembled_end);
- $self->write(@ff, '', '/note="contig '.$tile->component_Seq->name .
- ' ' . $tile->component_start . '..' . $tile->component_end.
- '(' . $tile->component_ori . ')"');
+ if($self->is_enabled('contig')) {
+ foreach my $segment (@{$slice->project('seqlevel')}) {
+ my ($start, $end, $slice) = @$segment;
+ $self->write(@ff, 'misc_feature',
+ $start .'..'. $end);
+ $self->write(@ff, '', '/note="contig '.$slice->seq_region_name .
+ ' ' . $slice->start . '..' . $slice->end .
+ '(' . $slice->strand . ')"');
}
}
@@ -793,9 +855,13 @@ sub dump_fasta {
my $slice = shift;
my $FH = shift;
-
- my $species =
- $slice->adaptor->db->get_MetaContainer->get_Species->binomial();
+ my $species;
+ my $spec = $slice->adaptor->db->get_MetaContainer->get_Species();
+ if($spec) {
+ $species = $spec->binomial();
+ } else {
+ $species = '';
+ }
my $name = $slice->name;
my $start = 1;
@@ -835,75 +901,43 @@ sub dump_fasta {
sub features2location {
my $self = shift;
my $features = shift;
-
+
my @join = ();
foreach my $f (@$features) {
- my $ctg = $f->contig;
+ my $slice = $f->slice;
+ my $start = $f->start();
+ my $end = $f->end();
+ my $strand = $f->strand();
- if($ctg->isa('Bio::EnsEMBL::Slice')) {
- if($f->start >= 1 && $f->end <= $ctg->length) {
- #this feature in on a slice and doesn't lie outside the boundary
+ if($start >= 1 && $end <= $slice->length) {
+ #this feature in on a slice and doesn't lie outside the boundary
- if($f->strand() == 1) {
- push @join, $f->start()."..".$f->end();
- } else {
- push @join, "complement(".$f->start()."..".$f->end().")";
- }
+ if($strand == 1) {
+ push @join, "$start..$end";
} else {
- my @fs = ();
- #this feature is outside the boundary of the dump,
- #convert to clone coords
- if($ctg->isa('Bio::EnsEMBL::Slice')) {
- #copy the feature, and transform to contig coords
- my $new_f;
- %$new_f = %$f;
- bless $new_f, ref $f;
- push @fs, $new_f->transform;
- }
-
- if($fs[0]->isa('Bio::EnsEMBL::StickyExon')) {
- @fs = @{$fs[0]->get_all_component_Exons};
- }
-
- #use the accession:x-y format
- foreach my $feat (@fs) {
- my $contig = $feat->contig;
- my $clone = $contig->clone;
- my $acc;
- if($clone->embl_id) {
- $acc = $clone->embl_id .'.'. $clone->embl_version;
- } else {
- $acc = $clone->id;
- }
- my $start = $feat->start + $contig->embl_offset - 1;
- my $end = $feat->end + $contig->embl_offset - 1;
- my $strand = $feat->strand;
- if($strand == 1) {
- push @join, "$acc:$start..$end";
- } else {
- push @join, "complement($acc:$start..$end)";
- }
- }
+ push @join, "complement($start..$end)";
}
} else {
- #handle features in contig coordinates
-
- my $start = $f->start;
- my $end = $f->end;
-
- if($start < 1 || $end > $ctg->length) {
- $self->throw("Feature off end of contig boundary");
- }
-
- if($f->strand == -1) {
- push @join, "complement($start..$end)";
- } else {
- push @join, "($start..$end)";
+ my @fs = ();
+ #this feature is outside the boundary of the dump,
+ #XXX TBD This should probably be CLONE coords but 2 step mapping is not
+ # yet implemented and 'seqlevel' is guaranteed to be 1step
+ my $projection = $f->project('seqlevel');
+ foreach my $segment (@$projection) {
+ my $slice = $segment->[2];
+ my $slc_start = $slice->start();
+ my $slc_end = $slice->end();
+ my $seq_reg = $slice->seq_region_name();
+ if($slice->strand == 1) {
+ push @join, "$seq_reg:$slc_start..$slc_end";
+ } else {
+ push @join, "complement($seq_reg:$slc_start..$slc_end)";
+ }
}
}
}
-
+
my $out = join ',', @join;
if(scalar @join > 1) {
diff --git a/modules/t/MultiTestDB.conf b/modules/t/MultiTestDB.conf
index df7cb3341f..8b38b0b0c1 100644
--- a/modules/t/MultiTestDB.conf
+++ b/modules/t/MultiTestDB.conf
@@ -1,13 +1,15 @@
{
- 'port' => 3307,
+ 'port' => 3306,
'driver' => 'mysql',
'user' => 'ensadmin',
'pass' => 'ensembl',
- 'host' => 'ecs3d',
+ 'host' => 'ecs1c',
'zip' => 'test_genome.zip',
#add a line with the dbname and module
- 'databases' => { 'core' => 'Bio::EnsEMBL::DBSQL::DBAdaptor'}
-
+ 'databases' => { 'core' => 'Bio::EnsEMBL::DBSQL::DBAdaptor'},
+#uncomment to use preloaded databases (useful when doing lots of testing)
+# 'preloaded' => { 'core' => 'arne_new_test' }
+
}
diff --git a/modules/t/MultiTestDB.pm b/modules/t/MultiTestDB.pm
index e8ddd6c484..695dfec00f 100644
--- a/modules/t/MultiTestDB.pm
+++ b/modules/t/MultiTestDB.pm
@@ -1,4 +1,4 @@
-
+
=pod
@@ -15,11 +15,9 @@
package MultiTestDB;
-use vars qw(@ISA %ENV);
-
-use Bio::EnsEMBL::Root;
+use vars qw(%ENV);
-@ISA = ('Bio::EnsEMBL::Root');
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
use strict;
@@ -150,7 +148,7 @@ sub create_adaptors {
};
if ($@) {
- $self->warn("WARNING: Could not instantiate $dbtype DBAdaptor:\n$@");
+ warning("WARNING: Could not instantiate $dbtype DBAdaptor:\n$@");
} else {
$self->{'db_adaptors'}->{$dbtype} = $adaptor;
}
@@ -158,6 +156,9 @@ sub create_adaptors {
}
+
+
+
sub load_databases {
my ($self) = shift;
@@ -176,105 +177,129 @@ sub load_databases {
#create a config hash which will be frozen to a file
$self->{'conf'} = {};
- #unzip database files
- $self->unzip_test_dbs($self->curr_dir . $zip);
+ #only unzip if there are non-preloaded datbases
+ UNZIP: foreach my $dbtype (keys %{$db_conf->{'databases'}}) {
+ if( !$db_conf->{'preloaded'}->{$dbtype} ) {
+ #unzip database files
+ $self->unzip_test_dbs($self->curr_dir . $zip);
+ last UNZIP;
+ }
+ }
#connect to the database
my $locator = "DBI:".$driver.":host=".$host.";port=".$port;
my $db = DBI->connect($locator, $user, $pass, {RaiseError => 1});
unless($db) {
- $self->warn("Can't connect to database $locator");
+ warning("Can't connect to database $locator");
return;
}
#create a database for each database specified
foreach my $dbtype (keys %{$db_conf->{'databases'}}) {
- #create a unique random dbname
- my $dbname = $self->_create_db_name($dbtype);
+ #don't create a database if there is a preloaded one specified
+ if( $db_conf->{'preloaded'}->{$dbtype} ) {
+ #copy the general config into a dbtype specific config
+ $self->{'conf'}->{$dbtype} = {};
+ %{$self->{'conf'}->{$dbtype}} = %$db_conf;
+ $self->{'conf'}->{$dbtype}->{'module'} = $db_conf->{'databases'}->{$dbtype};
- print STDERR "\nCreating db $dbname";
+ # it's not necessary to store the databases and zip bits of info
+ delete $self->{'conf'}->{$dbtype}->{'databases'};
+ delete $self->{'conf'}->{$dbtype}->{'zip'};
- unless($db->do("CREATE DATABASE $dbname")) {
- $self->warn("Could not create database [$dbname]");
- return;
- }
- #copy the general config into a dbtype specific config
- $self->{'conf'}->{$dbtype} = {};
- %{$self->{'conf'}->{$dbtype}} = %$db_conf;
- $self->{'conf'}->{$dbtype}->{'module'} = $db_conf->{'databases'}->{$dbtype};
+ #store the temporary database name in the dbtype specific config
+ $self->{'conf'}->{$dbtype}->{'dbname'} = $db_conf->{'preloaded'}->{$dbtype};
+ $self->{'conf'}->{$dbtype}->{'preloaded'} = 1;
+ } else {
- # it's not necessary to store the databases and zip bits of info
- delete $self->{'conf'}->{$dbtype}->{'databases'};
- delete $self->{'conf'}->{$dbtype}->{'zip'};
+ #create a unique random dbname
+ my $dbname = $self->_create_db_name($dbtype);
+
+ print STDERR "\nCreating db $dbname";
+
+ unless($db->do("CREATE DATABASE $dbname")) {
+ warning("Could not create database [$dbname]");
+ return;
+ }
+ #copy the general config into a dbtype specific config
+ $self->{'conf'}->{$dbtype} = {};
+ %{$self->{'conf'}->{$dbtype}} = %$db_conf;
+ $self->{'conf'}->{$dbtype}->{'module'} = $db_conf->{'databases'}->{$dbtype};
- #store the temporary database name in the dbtype specific config
- $self->{'conf'}->{$dbtype}->{'dbname'} = $dbname;
+ # it's not necessary to store the databases and zip bits of info
+ delete $self->{'conf'}->{$dbtype}->{'databases'};
+ delete $self->{'conf'}->{$dbtype}->{'preloaded'};
+ delete $self->{'conf'}->{$dbtype}->{'zip'};
- $db->do("use $dbname");
-
- #load the database with data
- my $dir = $self->curr_dir . "$DUMP_DIR/".$self->species."/$dbtype";
- local *DIR;
- unless(opendir(DIR, $dir)) {
- $self->warn("could not open dump directory '$dir'");
- return;
- }
+ #store the temporary database name in the dbtype specific config
+ $self->{'conf'}->{$dbtype}->{'dbname'} = $dbname;
- my @files = readdir DIR;
+ $db->do("use $dbname");
+
+ #load the database with data
+ my $dir = $self->curr_dir . "$DUMP_DIR/".$self->species."/$dbtype";
+ local *DIR;
- local *FILE;
+ unless(opendir(DIR, $dir)) {
+ warning("could not open dump directory '$dir'");
+ return;
+ }
- #read in table creat statements from *.sql files and process them with DBI
+ my @files = readdir DIR;
- foreach my $sql_file (grep /\.sql$/, @files) {
+ local *FILE;
- $sql_file = "$dir/$sql_file";
+ #read in table creat statements from *.sql files and process them with DBI
- unless(-f $sql_file && -r $sql_file) {
- $self->warn("could not read SQL file '$sql_file'\n");
- next;
- }
+ foreach my $sql_file (grep /\.sql$/, @files) {
+
+ $sql_file = "$dir/$sql_file";
+
+ unless(-f $sql_file && -r $sql_file) {
+ warning("could not read SQL file '$sql_file'\n");
+ next;
+ }
- open(FILE, $sql_file);
+ open(FILE, $sql_file);
+
+ my $sql_com ='';
+
+ while (<FILE>) {
+ next if ( /^#/ ); # ignore comments
+ next unless ( /\S/ ); # ignore lines of white spaces
- my $sql_com ='';
+ $sql_com .= $_;
+ }
+ $sql_com =~ s/;$//; # chop off the last ;
- while (<FILE>) {
- next if ( /^#/ ); # ignore comments
- next unless ( /\S/ ); # ignore lines of white spaces
+ $db->do($sql_com);
- $sql_com .= $_;
- }
- $sql_com =~ s/;$//; # chop off the last ;
+ close FILE;
- $db->do($sql_com);
+ #import data from the txt files of the same name
+ $sql_file =~ /.*\/(.*)\.sql/;
+ my $tablename = $1;
- close FILE;
+ (my $txt_file = $sql_file) =~ s/\.sql$/\.txt/;
- #import data from the txt files of the same name
- $sql_file =~ /.*\/(.*)\.sql/;
- my $tablename = $1;
+ unless(-f $txt_file && -r $txt_file) {
+ warning("could not read data file '$txt_file'\n");
+ next;
+ }
- (my $txt_file = $sql_file) =~ s/\.sql$/\.txt/;
+ $db->do( "load data local infile '$txt_file' into table $tablename" );
- unless(-f $txt_file && -r $txt_file) {
- $self->warn("could not read data file '$txt_file'\n");
- next;
}
-
- $db->do( "load data local infile '$txt_file' into table $tablename" );
-
}
- }
print STDERR "\n";
- closedir DIR;
-
- $db->disconnect;
+ closedir DIR;
+ $db->disconnect;
+ }
}
@@ -282,16 +307,16 @@ sub unzip_test_dbs {
my ($self, $zipfile) = @_;
if (-e $self->curr_dir . $DUMP_DIR) {
- $self->warn("Test genome dbs already unpacked\n");
+ warning("Test genome dbs already unpacked\n");
return;
}
unless($zipfile) {
- $self->throw("zipfile argument is required\n");
+ throw("zipfile argument is required\n");
}
unless(-f $zipfile) {
- $self->warn("zipfile could not be found\n");
+ warning("zipfile could not be found\n");
return;
}
@@ -311,7 +336,7 @@ sub get_DBAdaptor {
}
unless($self->{'db_adaptors'}->{$type}) {
- $self->warn("dbadaptor of type $type is not available\n");
+ warning("dbadaptor of type $type is not available\n");
return undef;
}
@@ -342,7 +367,7 @@ sub get_DBAdaptor {
sub hide {
my ($self, $dbtype, @tables) = @_;
-
+
unless($dbtype && @tables) {
die("dbtype and table args must be defined\n");
}
@@ -354,44 +379,27 @@ sub hide {
}
foreach my $table (@tables) {
+
if($self->{'conf'}->{$dbtype}->{'hidden'}->{$table}) {
- $self->warn("table '$table' is already hidden and cannot be hidden again\n");
+ warning("table '$table' is already hidden and cannot be hidden again\n");
next;
}
my $hidden_name = "_hidden_$table";
- #do some table renaming sql
- my $sth = $adaptor->prepare("alter table $table rename $hidden_name");
-
- $sth->execute;
-
- #reload the old table from its schema file
- my $schema_file = $self->curr_dir . "$DUMP_DIR/" . $self->species . "/$dbtype/$table.sql";
-
- local *SCHEMA_FILE;
-
- unless(-f $schema_file && -e $schema_file &&
- open (SCHEMA_FILE,$schema_file) ) {
- #rename the table back
- $sth = $adaptor->prepare("alter table $hidden_name rename $table");
- $sth->execute;
- $self->warn("could not read schema file '$schema_file' for $dbtype $table" .
- ". table could not be hidden");
- next;
- }
-
- #read all the lines from the schema definition
- my @lines = <SCHEMA_FILE>;
- my $sql = join ' ', @lines;
+ #copy contents of table into a temp table
- $sql =~ s/;\s*$//;
+ my $sth =
+ $adaptor->prepare("CREATE TABLE $hidden_name " .
+ "SELECT * FROM $table");
- close SCHEMA_FILE;
+ $sth->execute();
+ $sth->finish();
- #presumably create the **empty** table
- $sth = $adaptor->prepare($sql);
- $sth->execute;
+ #delete the contents of the original table
+ $sth = $adaptor->prepare("DELETE FROM $table");
+ $sth->execute();
+ $sth->finish();
#update the hidden table config
$self->{'conf'}->{$dbtype}->{'hidden'}->{$table} = $hidden_name;
@@ -419,8 +427,8 @@ sub hide {
sub restore {
my ($self, $dbtype, @tables) = @_;
-
- unless($dbtype) {
+
+ if(!$dbtype) {
#restore all of the tables in every dbtype
foreach my $dbtype (keys %{$self->{'conf'}}) {
@@ -428,7 +436,7 @@ sub restore {
}
#lose the hidden table details
-# delete $self->{'conf'}->{'hidden'};
+ delete $self->{'conf'}->{'hidden'};
return;
}
@@ -437,8 +445,8 @@ sub restore {
unless($adaptor) {
die "Adaptor for $dbtype is not available";
}
-
- unless(@tables) {
+
+ if(!@tables) {
#restore all of the tables for this db
@tables = keys %{$self->{'conf'}->{$dbtype}->{'hidden'}};
}
@@ -446,18 +454,25 @@ sub restore {
foreach my $table (@tables) {
my $hidden_name = $self->{'conf'}->{$dbtype}->{'hidden'}->{$table};
- #drop existing table
- my $sth = $adaptor->prepare("drop table $table");
- $sth->execute;
-
- #rename hidden table
- $sth = $adaptor->prepare("alter table $hidden_name rename $table");
- $sth->execute;
+ #delete current contents of table
+ my $sth = $adaptor->prepare("delete from $table");
+ $sth->execute();
+ $sth->finish();
+
+ #copy contents of tmp table back into main table
+ $sth = $adaptor->prepare("insert into $table " .
+ "select * from $hidden_name");
+ $sth->execute();
+ $sth->finish();
+
+ #drop temp table
+ $sth = $adaptor->prepare("drop table $hidden_name");
+ $sth->execute();
+ $sth->finish();
#delete value from hidden table config
delete $self->{'conf'}->{$dbtype}->{'hidden'}->{$table};
}
-
}
=head2 save
@@ -502,11 +517,12 @@ sub save {
$hidden_name = "_hidden_$table";
#copy the data from the hidden table into the new table
- my $sth = $adaptor->prepare("insert into $table select * from $hidden_name");
+ my $sth = $adaptor->prepare("insert into $table " .
+ "select * from $hidden_name");
$sth->execute;
}
else {
- $self->warn("hidden table '$hidden_name' does not exist so saving is not possible\n");
+ warning("hidden table '$hidden_name' does not exist so saving is not possible\n");
}
}
}
@@ -514,7 +530,7 @@ sub save {
sub compare {
my ($self, $dbtype, $table) = @_;
- $self->warn("save method not yet implemented\n");
+ warning("save method not yet implemented\n");
}
@@ -585,6 +601,9 @@ sub cleanup {
my $driver = $db_conf->{'driver'};
my $dbname = $db_conf->{'dbname'};
+ if( $db_conf->{'preloaded'} ) {
+ next;
+ }
#connect to the database
my $locator = "DBI:".$driver.":host=".$host.";port=".$port;
@@ -646,6 +665,9 @@ sub DESTROY {
} else {
#we are runnning a stand-alone test, cleanup created databases
print STDERR "\nCleaning up....\n";
+ #restore database state since we may not actually delete it in the
+ #cleanup - it may be defined as a preloaded db
+ $self->restore;
$self->cleanup;
}
}
diff --git a/modules/t/argument.t b/modules/t/argument.t
new file mode 100644
index 0000000000..0be78206a5
--- /dev/null
+++ b/modules/t/argument.t
@@ -0,0 +1,25 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 3;
+}
+
+use TestUtils qw( debug );
+
+use Bio::EnsEMBL::Utils::Argument qw(rearrange);
+
+our $verbose= 0;
+
+my @args = ('-TWO' => 2,
+ '-ONE' => 1,
+ '-THREE' => 3);
+
+my ($one, $two, $three) = rearrange(['ONE','TWO','THREE'],@args);
+
+ok($one == 1);
+ok($two == 2);
+ok($three == 3);
diff --git a/modules/t/assemblyException.t b/modules/t/assemblyException.t
new file mode 100644
index 0000000000..8a0ae822fc
--- /dev/null
+++ b/modules/t/assemblyException.t
@@ -0,0 +1,136 @@
+use lib 't';
+use strict;
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 7;
+}
+
+
+use MultiTestDB;
+
+use Bio::EnsEMBL::DnaDnaAlignFeature;
+use TestUtils qw(test_getter_setter debug);
+
+our $verbose = 0;
+
+my $multi = MultiTestDB->new();
+ok(1);
+
+my $db = $multi->get_DBAdaptor('core');
+my $sfa = $db->get_SimpleFeatureAdaptor();
+my $slice_adaptor = $db->get_SliceAdaptor();
+
+##chromosome Y is a fake 'PAR' linked to chromosome 20
+my $slice = $slice_adaptor->fetch_by_region('chromosome', 'Y',8e6,13e6);
+my $feats = $sfa->fetch_all_by_Slice($slice);
+debug("Got " . scalar(@$feats));
+ok( @$feats ==58 );
+
+print_features($feats);
+
+
+#HAP_1 is a fake haplotype on chromosome 20
+$slice = $slice_adaptor->fetch_by_region('chromosome', '20_HAP1',
+ 30_399_998,30_600_002);
+my $org_slice = $slice_adaptor->fetch_by_region('chromosome', '20',
+ 30_430_000,30_500_000 );
+
+
+$feats = $sfa->fetch_all_by_Slice($slice);
+
+debug("Got " . scalar(@$feats));
+ok( @$feats == 9 );
+
+print_features($feats);
+
+$multi->hide( "core", "simple_feature" );
+$multi->save( "core", "meta_coord" );
+
+for my $f ( @$feats ) {
+ $f->dbID( undef );
+ $f->adaptor( undef );
+ $sfa->store( $f );
+
+ $f->dbID( undef );
+ $f->adaptor( undef );
+ $f->slice( $org_slice );
+ $sfa->store( $f );
+
+}
+
+
+$slice = $slice_adaptor->fetch_by_region('chromosome', '20_HAP1',
+ 30_400_000,30_600_000);
+$feats = $sfa->fetch_all_by_Slice( $slice );
+
+debug( "After storing retrieval" );
+print_features($feats);
+ok(@$feats == 14);
+
+
+
+#
+# sequence getting tests
+#
+
+my $hap_slice = $slice_adaptor->fetch_by_region('chromosome', '20_HAP1',
+ 30_400_000,30_700_000 );
+
+$org_slice = $slice_adaptor->fetch_by_region('chromosome', '20',
+ 30_400_000,30_800_000 );
+
+my ( $fhs, $bhs, $fos, $bos );
+
+debug( "Front hap seq: ".($fhs = $hap_slice->subseq( 99_991, 100_000 )));
+debug( "Back hap seq: ".($bhs = $hap_slice->subseq( 200_001, 200_010 )));
+debug( "Front org seq: ".( $fos = $org_slice->subseq( 99_991, 100_000 )));
+debug( "Back org seq: ".( $bos = $org_slice->subseq( 300_001, 300_010 )));
+
+ok( $fhs eq $fos );
+ok( $bhs eq $bos );
+
+
+$slice = $slice_adaptor->fetch_by_region('chromosome', '20_HAP1',
+ 30_499_998,30_500_002);
+
+debug($slice->seq);
+ok( $slice->seq() eq "GTNNN" );
+
+$multi->restore();
+
+
+#try projecting a hap slice to the contig coordinate system
+debug("Org slice projection");
+my $projection = $org_slice->project('contig');
+print_projection($projection);
+
+debug("Hap slice projection");
+$projection = $hap_slice->project('contig');
+print_projection($projection);
+
+
+sub print_features {
+ return if(!$verbose);
+ my $features = shift;
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ my $seqname = $f->slice->seq_region_name();
+ my $analysis = $f->analysis->logic_name();
+ debug($seqname . ' ' . $f->start().'-'.$f->end().'('.$f->strand().
+ ') ['. $f->dbID.'] '.$f->score() ." ($analysis) ");
+ } else {
+ debug('UNDEF');
+ }
+ }
+}
+
+
+
+sub print_projection {
+ my $proj = shift;
+ foreach my $seg (@$proj) {
+ my ($start, $end, $seq_reg) = ($seg->[0],$seg->[1],$seg->[2]->seq_region_name());
+ debug("[$start-$end] $seq_reg");
+ }
+}
diff --git a/modules/t/assemblyExceptionFeature.t b/modules/t/assemblyExceptionFeature.t
new file mode 100644
index 0000000000..0428bff67f
--- /dev/null
+++ b/modules/t/assemblyExceptionFeature.t
@@ -0,0 +1,63 @@
+use strict;
+
+use lib 't';
+use TestUtils qw(test_getter_setter debug);
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 8;
+}
+
+use MultiTestDB;
+use Bio::EnsEMBL::AssemblyExceptionFeature;
+
+
+our $verbose = 0;
+
+my $multi = MultiTestDB->new;
+
+# get a core DBAdaptor
+#
+my $dba = $multi->get_DBAdaptor("core");
+my $aefa = $dba->get_AssemblyExceptionFeatureAdaptor();
+ok($aefa);
+
+#
+# 1 create a new AssemblyExceptionFeature
+#
+my $aef = new Bio::EnsEMBL::AssemblyExceptionFeature;
+ok($aef);
+
+#
+# test the basic getter and setters
+#
+
+# start
+ok(test_getter_setter($aef,'start',10));
+
+# end
+ok(test_getter_setter($aef,'end',14));
+
+# type
+ok(test_getter_setter($aef,'type', 'HAP'));
+
+# check adaptor attaching
+$aef->adaptor($aefa);
+ok($aef->adaptor->isa('Bio::EnsEMBL::DBSQL::AssemblyExceptionFeatureAdaptor'));
+
+# fetch all
+my $chr_slice = $dba->get_SliceAdaptor->fetch_by_region('chromosome',
+ '20_HAP1');
+my @features = @{$aefa->fetch_all_by_Slice($chr_slice)};
+
+ok(@features);
+foreach my $f (@features) {
+ debug( "Feature: " . $f->slice->seq_region_name . " " .
+ $f->start . " " . $f->end . " " . $f->type);
+ my $as = $f->alternate_slice();
+ debug(" Alternate slice: " . $as->seq_region_name . " " .
+ $as->start . " " . $as->end);
+}
+
+my ($f) = @features;
+ok($f->display_id eq $f->alternate_slice->seq_region_name);
diff --git a/modules/t/assemblyMapper.t b/modules/t/assemblyMapper.t
new file mode 100644
index 0000000000..244f4d47ec
--- /dev/null
+++ b/modules/t/assemblyMapper.t
@@ -0,0 +1,113 @@
+use lib 't';
+use strict;
+
+BEGIN { $| = 1;
+ use Test ;
+ plan tests => 9;
+}
+
+use MultiTestDB;
+use TestUtils qw(debug test_getter_setter);
+
+our $verbose = 0; #set to 1 to turn on debug printouts
+
+my $multi = MultiTestDB->new();
+my $db = $multi->get_DBAdaptor( 'core' );
+
+#
+# 1 Test AssemblyMapperAdaptor constructor
+#
+my $asma = $db->get_AssemblyMapperAdaptor();
+
+
+ok($asma && $asma->isa('Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor'));
+
+
+#
+# 2 Test fetch_by_CoordSystems
+#
+
+my $csa = $db->get_CoordSystemAdaptor();
+my $ctg_cs = $csa->fetch_by_name('contig');
+my $chr_cs = $csa->fetch_by_name('chromosome');
+my $cln_cs = $csa->fetch_by_name('clone');
+
+my $asm_mapper = $asma->fetch_by_CoordSystems($ctg_cs, $chr_cs);
+
+ok($asm_mapper && $asm_mapper->isa('Bio::EnsEMBL::AssemblyMapper'));
+
+
+#
+# test db has chr 20 (50KB -> 62MB)
+#
+
+#
+# 3 Test map
+#
+
+my @coords = $asm_mapper->map('20', 500_001, 60_000_000, 1, $chr_cs);
+ok(@coords);
+print_coords(@coords);
+
+
+@coords = $asm_mapper->map('AL359765.6.1.13780', 1, 13780, 1, $ctg_cs);
+ok(@coords);
+print_coords(@coords);
+
+my $cln_mapper = $asma->fetch_by_CoordSystems($cln_cs, $ctg_cs);
+@coords = $cln_mapper->map('AL359765.6', 1, 20_000, 1, $cln_cs);
+ok(@coords);
+print_coords(@coords);
+
+
+#
+# Test list_seq_regions
+#
+
+my @seq_regions =
+ $asm_mapper->list_seq_regions('20', 500_001, 60_000_000, $chr_cs);
+ok(@seq_regions);
+my $str = join("\n", "----------", @seq_regions);
+debug("$str\n");
+
+@seq_regions =
+ $asm_mapper->list_seq_regions('AL359765.6.1.13780', 1, 13780, $ctg_cs);
+ok(@seq_regions);
+$str = join("\n", "----------", @seq_regions);
+debug("$str\n");
+
+
+#
+# Test list_seq_ids
+#
+
+
+my @seq_ids =
+ $asm_mapper->list_ids('20', 500_001, 60_000_000, $chr_cs);
+ok(@seq_ids);
+$str = join("\n", "----------", @seq_ids);
+debug("$str\n");
+
+@seq_ids =
+ $asm_mapper->list_ids('AL359765.6.1.13780', 1, 13780, $ctg_cs);
+ok(@seq_ids);
+$str = join("\n", "----------", @seq_ids);
+debug("$str\n");
+
+
+
+sub print_coords {
+ my @coord_list = @_;
+
+ return if(!$verbose);
+
+ foreach my $coord (@coord_list) {
+ if($coord->isa('Bio::EnsEMBL::Mapper::Gap')) {
+ debug("GAP");
+ next;
+ }
+ debug($coord->id()."\t". $coord->start()."-".$coord->end().
+ " (".$coord->strand.")");
+ }
+}
+
diff --git a/modules/t/canonicalDBAdaptor.t b/modules/t/canonicalDBAdaptor.t
index f67e67bd3f..02414adac4 100644
--- a/modules/t/canonicalDBAdaptor.t
+++ b/modules/t/canonicalDBAdaptor.t
@@ -5,7 +5,7 @@ use lib 't';
BEGIN { $| = 1;
use Test;
- plan tests => 41;
+ plan tests => 38;
}
@@ -35,12 +35,12 @@ $test_adaptor = $db->get_QtlAdaptor();
ok($test_adaptor->isa("Bio::EnsEMBL::Map::DBSQL::QtlAdaptor"));
$test_adaptor = $db->get_ProteinFeatureAdaptor();
ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor"));
-$test_adaptor = $db->get_ProteinAdaptor();
-ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::ProteinAdaptor"));
+#$test_adaptor = $db->get_ProteinAdaptor();
+#ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::ProteinAdaptor"));
$test_adaptor = $db->get_MapFragAdaptor();
ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::MapFragAdaptor"));
-$test_adaptor = $db->get_CloneAdaptor();
-ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::CloneAdaptor"));
+#$test_adaptor = $db->get_CloneAdaptor();
+#ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::CloneAdaptor"));
$test_adaptor = $db->get_PredictionTranscriptAdaptor();
ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::PredictionTranscriptAdaptor"));
$test_adaptor = $db->get_SequenceAdaptor();
@@ -53,8 +53,8 @@ $test_adaptor = $db->get_TranscriptAdaptor();
ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::TranscriptAdaptor"));
$test_adaptor = $db->get_TranslationAdaptor();
ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::TranslationAdaptor"));
-$test_adaptor = $db->get_RawContigAdaptor();
-ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::RawContigAdaptor"));
+#$test_adaptor = $db->get_RawContigAdaptor();
+#ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::RawContigAdaptor"));
$test_adaptor = $db->get_SliceAdaptor();
ok($test_adaptor->isa("Bio::EnsEMBL::DBSQL::SliceAdaptor"));
$test_adaptor = $db->get_AnalysisAdaptor();
@@ -132,7 +132,7 @@ eval { my %generic_adpators = $db->get_GenericFeatureAdaptors("Mickey") };
ok($@);
# Slice tests - should these go in slice.t?
-my $slice = $db->get_SliceAdaptor()->fetch_by_chr_start_end('X', 1, 10000);
+my $slice = $db->get_SliceAdaptor()->fetch_by_region('chromosome','X',1,10000);
ok($slice);
my %features = %{$slice->get_generic_features()};
diff --git a/modules/t/chainedAssemblyMapper.t b/modules/t/chainedAssemblyMapper.t
new file mode 100644
index 0000000000..46c421fe13
--- /dev/null
+++ b/modules/t/chainedAssemblyMapper.t
@@ -0,0 +1,146 @@
+use lib 't';
+use strict;
+
+BEGIN { $| = 1;
+ use Test ;
+ plan tests => 14;
+}
+
+use MultiTestDB;
+use TestUtils qw(debug test_getter_setter);
+
+our $verbose = 0; #set to 1 to turn on debug printouts
+
+my $multi = MultiTestDB->new();
+my $db = $multi->get_DBAdaptor( 'core' );
+
+#
+# 1 Test AssemblyMapperAdaptor constructor
+#
+my $asma = $db->get_AssemblyMapperAdaptor();
+
+
+ok($asma && $asma->isa('Bio::EnsEMBL::DBSQL::AssemblyMapperAdaptor'));
+
+
+#
+# 2 Test fetch_by_CoordSystems
+#
+
+my $csa = $db->get_CoordSystemAdaptor();
+my $chr_cs = $csa->fetch_by_name('chromosome');
+my $cln_cs = $csa->fetch_by_name('clone');
+my $sctg_cs = $csa->fetch_by_name('supercontig');
+
+my $asm_mapper = $asma->fetch_by_CoordSystems($cln_cs, $chr_cs);
+
+ok($asm_mapper && $asm_mapper->isa('Bio::EnsEMBL::ChainedAssemblyMapper'));
+
+my $chr_sctg_mapper = $asma->fetch_by_CoordSystems($chr_cs, $sctg_cs);
+ok($chr_sctg_mapper &&
+ $chr_sctg_mapper->isa('Bio::EnsEMBL::ChainedAssemblyMapper'));
+
+
+
+
+#
+# test db has chr 20 (50KB -> 62MB)
+#
+
+#
+# 3 Test map
+#
+
+my @coords = $asm_mapper->map('20', 500_001, 60_000_000, 1, $chr_cs);
+ok(@coords);
+debug("MAP 20->clone\n");
+print_coords(@coords);
+
+debug("MAP 'AL359765.6'->chromosome\n");
+@coords = $asm_mapper->map('AL359765.6', 1, 13780, 1, $cln_cs);
+ok(@coords);
+print_coords(@coords);
+
+debug("MAP 20->supercontig\n");
+@coords = $chr_sctg_mapper->map('20', 500_001, 60_000_000, 1, $chr_cs);
+ok(@coords);
+print_coords(@coords);
+
+
+#
+# Test list_seq_regions
+#
+
+my @seq_regions =
+ $asm_mapper->list_seq_regions('20', 500_001, 60_000_000, $chr_cs);
+ok(@seq_regions);
+my $str = join("\n", "----------", @seq_regions);
+debug("$str\n");
+
+@seq_regions =
+ $asm_mapper->list_seq_regions('AL359765.6', 1, 13780, $cln_cs);
+ok(@seq_regions);
+$str = join("\n", "----------", @seq_regions);
+debug("$str\n");
+
+@seq_regions =
+ $chr_sctg_mapper->list_seq_regions('NT_028392',600_000, 1_000_000, $sctg_cs);
+ok(@seq_regions);
+$str = join("\n", "----------", @seq_regions);
+debug("$str\n");
+
+
+@seq_regions =
+ $chr_sctg_mapper->list_seq_regions('20', 30_000_000, 31_000_000, $chr_cs);
+ok(@seq_regions);
+$str = join("\n", "----------", @seq_regions);
+debug("$str\n");
+
+
+#
+# Test list_seq_ids
+#
+
+
+my @seq_ids =
+ $asm_mapper->list_ids('20', 500_001, 60_000_000, $chr_cs);
+ok(@seq_ids);
+$str = join("\n", "----------", @seq_ids);
+debug("$str\n");
+
+@seq_ids =
+ $asm_mapper->list_ids('AL359765.6', 1, 13780, $cln_cs);
+ok(@seq_ids);
+$str = join("\n", "----------", @seq_ids);
+debug("$str\n");
+
+@seq_ids =
+ $asm_mapper->list_ids('AL359765.6', 1, 13780, $cln_cs);
+ok(@seq_ids);
+$str = join("\n", "----------", @seq_ids);
+debug("$str\n");
+
+@seq_ids =
+ $chr_sctg_mapper->list_ids('20', 30_000_000, 31_000_000, $chr_cs);
+ok(@seq_ids);
+$str = join("\n", "----------", @seq_ids);
+debug("$str\n");
+
+
+
+
+sub print_coords {
+ my @coord_list = @_;
+
+ return if(!$verbose);
+
+ foreach my $coord (@coord_list) {
+ if($coord->isa('Bio::EnsEMBL::Mapper::Gap')) {
+ debug("GAP");
+ next;
+ }
+ debug($coord->id()."\t". $coord->start()."-".$coord->end().
+ " (".$coord->strand.")");
+ }
+}
+
diff --git a/modules/t/chromosome.t b/modules/t/chromosome.t
index 1962120fd1..f8f2687914 100644
--- a/modules/t/chromosome.t
+++ b/modules/t/chromosome.t
@@ -5,15 +5,27 @@ use lib 't';
BEGIN { $| = 1;
use Test;
- plan tests => 8;
+ plan tests => 5;
}
use MultiTestDB;
use TestUtils qw(debug test_getter_setter);
+
+use Bio::EnsEMBL::Utils::Exception qw(verbose);
use Bio::EnsEMBL::Chromosome;
+######################################################################
+#
+# Chromosome is a deprecated class but needed for backwards
+# compatibility. These tests ensure that it actually works,
+# but verbosity is turned off to avoid all of the deprecated warnings
+#
+#######################################################################
+
+verbose(-1);
+
#
#1 TEST - Chromosome Compiles
#
@@ -30,7 +42,7 @@ my $db = $multi_db->get_DBAdaptor('core');
my $ca = $db->get_ChromosomeAdaptor;
#
-#2-8 Chromosome constructor
+# Chromosome constructor
#
my $chromosome = Bio::EnsEMBL::Chromosome->new
(-chr_name => $CHR,
@@ -39,15 +51,9 @@ my $chromosome = Bio::EnsEMBL::Chromosome->new
-length => $LENGTH);
ok($chromosome->isa('Bio::EnsEMBL::Chromosome'));
-ok($chromosome->length == $LENGTH);
-ok($chromosome->dbID == $DBID);
ok($chromosome->adaptor == $ca);
-
-#
-# 9-14 getter /setters
-#
-ok(test_getter_setter($chromosome, 'chr_name', 'Y'));
-ok(test_getter_setter($chromosome, 'dbID', 321));
-ok(test_getter_setter($chromosome, 'length', 123_000_000));
+ok($chromosome->length());
+ok($chromosome->name());
+verbose(0);
\ No newline at end of file
diff --git a/modules/t/clone.t b/modules/t/clone.t
index 0a90ff9995..a3b175ddfc 100644
--- a/modules/t/clone.t
+++ b/modules/t/clone.t
@@ -2,13 +2,25 @@ use lib 't';
use Test;
use strict;
-BEGIN { $| = 1; plan tests => 24;}
+BEGIN { $| = 1; plan tests => 6;}
use MultiTestDB;
use Bio::Seq;
use Bio::EnsEMBL::RawContig;
use Bio::EnsEMBL::Clone;
+use Bio::EnsEMBL::Utils::Exception qw(verbose);
+
+######################################################################
+#
+# Clone is a deprecated class but needed for backwards
+# compatibility. These tests ensure that it actually works,
+# but verbosity is turned off to avoid all of the deprecated warnings
+#
+#######################################################################
+
+verbose(-1);
+
ok(1);
# Database will be dropped when this
@@ -18,80 +30,82 @@ my $multi = MultiTestDB->new;
ok($multi);
#
-# get a core DBAdaptor
-#
+## get a core DBAdaptor
+##
my $dba = $multi->get_DBAdaptor("core");
-ok($dba);
-my $clone = Bio::EnsEMBL::Clone->new();
-ok($clone);
-
+#ok($dba);
-#
-# load the clone with some data
-#
-$clone->id('dummy_clone');
-$clone->dbID(24);
-$clone->embl_id('dummy_clone');
-$clone->embl_version(1);
-$clone->embl_id(42);
-$clone->version(1);
-$clone->htg_phase(3);
-$clone->created('2002-11-08 12:00:00');
-$clone->modified('2002-11-08 13:00:00');
+#my $clone = Bio::EnsEMBL::Clone->new();
+#ok($clone);
+
+
+##
+## load the clone with some data
+##
+
+#$clone->id('dummy_clone');
+#$clone->dbID(24);
+#$clone->embl_id('dummy_clone');
+#$clone->embl_version(1);
+#$clone->embl_id(42);
+#$clone->version(1);
+#$clone->htg_phase(3);
+#$clone->created('2002-11-08 12:00:00');
+#$clone->modified('2002-11-08 13:00:00');
-ok($clone);
+#ok($clone);
-#
-# create a dummy seq
-#
-my $seq = Bio::Seq->new(-seq => 'ATGCAGCTAGCATCGATGACATCG',
- -id => 'dummy_contig',
- -accession => 'dummy_contig');
-ok($seq);
+##
+## create a dummy seq
+##
+#my $seq = Bio::Seq->new(-seq => 'ATGCAGCTAGCATCGATGACATCG',
+# -id => 'dummy_contig',
+# -accession => 'dummy_contig');
+#ok($seq);
-my $contig1 = Bio::EnsEMBL::RawContig->new();
+#my $contig1 = Bio::EnsEMBL::RawContig->new();
-my $name = 'dummy_contig';
-$contig1->id($name);
-$contig1->embl_offset(0);
-$contig1->seq($seq);
-ok($contig1);
+#my $name = 'dummy_contig';
+#$contig1->id($name);
+#$contig1->embl_offset(0);
+#$contig1->seq($seq);
+#ok($contig1);
-#
-# add the contig to the clone
-#
-$clone->add_Contig($contig1);
-ok($clone);
+##
+## add the contig to the clone
+##
+#$clone->add_Contig($contig1);
+#ok($clone);
-#
-# create and add a 2nd contig
-#
-my $contig2 = Bio::EnsEMBL::RawContig->new();
-my $name2 = 'dummy_contig2';
-$contig2->id($name2);
-$contig2->embl_offset(0);
-$contig2->seq($seq);
+##
+## create and add a 2nd contig
+##
+#my $contig2 = Bio::EnsEMBL::RawContig->new();
+#my $name2 = 'dummy_contig2';
+#$contig2->id($name2);
+#$contig2->embl_offset(0);
+#$contig2->seq($seq);
-$clone->add_Contig($contig2);
-ok($clone);
+#$clone->add_Contig($contig2);
+#ok($clone);
-#
-# now retrieve the contigs
-#
-my $contigs = $clone->get_all_Contigs;
-ok(scalar(@$contigs) == 2);
+##
+## now retrieve the contigs
+##
+#my $contigs = $clone->get_all_Contigs;
+#ok(scalar(@$contigs) == 2);
-#
-# now let's get a real clone from the test db
+##
+## now let's get a real clone from the test db
#
my $clone_adaptor = $dba->get_CloneAdaptor;
ok($clone_adaptor);
@@ -104,117 +118,113 @@ ok($real_clone);
#
# get all the contigs on this clone
#
-$contigs = $real_clone->get_all_Contigs;
+my $contigs = $real_clone->get_all_Contigs;
ok(scalar(@$contigs) == 1);
#
# and get all the genes
#
-my $genes = $real_clone->get_all_Genes;
-ok(scalar(@$genes) == 5);
+#my $genes = $real_clone->get_all_Genes;
+#ok(scalar(@$genes) == 5);
+
+
+##
+## to test the delete method we need to save the tables that we delete from
+##
+
+#$multi->save("core","contig","clone","dna","repeat_feature","simple_feature",
+# "prediction_transcript","protein_align_feature","dna_align_feature");
+
+##
+## do the deletion
+##
+#$clone_adaptor->remove($real_clone);
+
+##
+## check the clones
+##
+#my $sth = $dba->prepare("select * from clone");
+#$sth->execute;
+##print STDERR "Num clones " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 11);
+
+##
+## check the contigs
+##$sth = $dba->prepare("select * from contig");
+#$sth->execute;
+##print STDERR "Num contigs " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 11);
+
+##
+## check the simple features
+##
+#$sth = $dba->prepare("select * from simple_feature");
+#$sth->execute;
+##print STDERR "Num simple_features " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 116);
+
+##
+## check the repeat features
+##
+#$sth = $dba->prepare("select * from repeat_feature");
+#$sth->execute;
+##print STDERR "Num repeat_features " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 1937);
+
+##
+## check the protein_align_features
+##
+#$sth = $dba->prepare("select * from protein_align_feature");
+#$sth->execute;
+##print STDERR "Num protein_align_features " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 4727);
+
+##
+## check the protein_align_features
+##
+#$sth = $dba->prepare("select * from dna_align_feature");
+#$sth->execute;
+##print STDERR "Num dna_align_features " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 15525);
+
+##
+## check the prediction_transcripts
+##
+#$sth = $dba->prepare("select * from prediction_transcript");
+#$sth->execute;
+##print STDERR "Num dna_align_features " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 161);
+
+
+##
+## check the dna
+##
+#$sth = $dba->prepare("select * from dna");
+#$sth->execute;
+##print STDERR "Num dna records " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 11);
+
+
+## restore the tables for the next test
+#$multi->restore("core","contig","clone","dna","repeat_feature","simple_feature",
+# "prediction_transcript","protein_align_feature","dna_align_feature");
+
+
+##
+## just a quick check to see whether the restore has worked
+##
+#$sth = $dba->prepare("select * from contig");
+#$sth->execute;
+#ok(scalar($sth->rows) == 12);
-#
-# to test the delete method we need to save the tables that we delete from
-#
-
-$multi->save("core","contig","clone","dna","repeat_feature","simple_feature",
- "prediction_transcript","protein_align_feature","dna_align_feature");
-
-#
-# do the deletion
-#
-$clone_adaptor->remove($real_clone);
-
-#
-# check the clones
-#
-my $sth = $dba->prepare("select * from clone");
-$sth->execute;
-#print STDERR "Num clones " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 11);
-$sth->finish();
-
-
-#
-# check the contigs
-$sth = $dba->prepare("select * from contig");
-$sth->execute;
-#print STDERR "Num contigs " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 11);
-$sth->finish();
-
-#
-# check the simple features
-#
-$sth = $dba->prepare("select * from simple_feature");
-$sth->execute;
-#print STDERR "Num simple_features " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 116);
-$sth->finish();
-
-#
-# check the repeat features
-#
-$sth = $dba->prepare("select * from repeat_feature");
-$sth->execute;
-#print STDERR "Num repeat_features " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 1937);
-$sth->finish();
-
-#
-# check the protein_align_features
-#
-$sth = $dba->prepare("select * from protein_align_feature");
-$sth->execute;
-#print STDERR "Num protein_align_features " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 4727);
-$sth->finish();
-
-#
-# check the protein_align_features
-#
-$sth = $dba->prepare("select * from dna_align_feature");
-$sth->execute;
-#print STDERR "Num dna_align_features " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 15525);
-$sth->finish();
-
-#
-# check the prediction_transcripts
-#
-$sth = $dba->prepare("select * from prediction_transcript");
-$sth->execute;
-#print STDERR "Num dna_align_features " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 161);
-
-
-#
-# check the dna
-#
-$sth = $dba->prepare("select * from dna");
-$sth->execute;
-#print STDERR "Num dna records " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 11);
-$sth->finish();
-
-# restore the tables for the next test
-$multi->restore("core","contig","clone","dna","repeat_feature","simple_feature",
- "prediction_transcript","protein_align_feature","dna_align_feature");
-
-
-#
-# just a quick check to see whether the restore has worked
-#
-$sth = $dba->prepare("select * from contig");
-$sth->execute;
-ok(scalar($sth->rows) == 12);
-$sth->finish();
-
#
# This is a snpview method
#
my $raw_contig = undef;
$raw_contig = $real_clone->get_RawContig_by_position(42);
ok($raw_contig);
+
+
+verbose(0);
\ No newline at end of file
diff --git a/modules/t/compressedSequenceAdaptor.t b/modules/t/compressedSequenceAdaptor.t
new file mode 100644
index 0000000000..0cc46276d7
--- /dev/null
+++ b/modules/t/compressedSequenceAdaptor.t
@@ -0,0 +1,73 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 3;
+}
+
+use TestUtils qw( debug );
+
+use MultiTestDB;
+use Bio::EnsEMBL::Slice;
+
+our $verbose= 0;
+
+my $multi_db = MultiTestDB->new;
+my $db = $multi_db->get_DBAdaptor('core');
+
+#
+# Test fetch_by_Slice_start_end_strand
+#
+my $slice_adaptor = $db->get_SliceAdaptor;
+my $seq_adaptor = $db->get_CompressedSequenceAdaptor();
+
+
+my $seq = 'ACTGAAANTTANNNATYTTTAAATTACCC';
+my $len = length($seq);
+
+my $contig_cs = $db->get_CoordSystemAdaptor->fetch_by_name('contig');
+
+$multi_db->save('core', 'dnac', 'seq_region');
+
+#we need to create a fake seq region because the length of the seq region
+#needs to match the length of the sequence we are inserting. Otherwise
+#we can get weird padding at the end due to the last byte being not
+#fully packed but still labelled as non-gap
+my $sth =
+ $db->prepare('INSERT INTO seq_region (name, length, coord_system_id) ' .
+ 'VALUES (?,?,?)');
+$sth->execute('testfrag', $len,$contig_cs->dbID);
+
+
+my $slice = $slice_adaptor->fetch_by_region('contig', 'testfrag');
+
+my $seq_region_id = $slice_adaptor->get_seq_region_id($slice);
+
+
+debug("Storing sequence: $seq");
+$seq_adaptor->store($seq_region_id,$seq);
+
+ok(1);
+
+my $new_seq = ${$seq_adaptor->fetch_by_Slice_start_end_strand($slice, 1,
+ $len, 1)};
+
+ok($seq eq $new_seq);
+
+
+debug("Retrieved sequence: $seq");
+
+my $flanking = 5;
+$new_seq = ${$seq_adaptor->fetch_by_Slice_start_end_strand($slice,
+ 1-$flanking,
+ $len+$flanking, 1)};
+
+
+ok(('N' x $flanking) . $seq . ('N' x $flanking) eq $new_seq);
+
+debug("Retrieved sequence (with $flanking flanking): $new_seq");
+
+$multi_db->restore('core', 'dnac', 'seq_region');
diff --git a/modules/t/coordSystem.t b/modules/t/coordSystem.t
new file mode 100644
index 0000000000..a5c3eece97
--- /dev/null
+++ b/modules/t/coordSystem.t
@@ -0,0 +1,87 @@
+use strict;
+use warnings;
+
+use lib 't';
+use Bio::EnsEMBL::CoordSystem;
+
+use TestUtils qw(debug test_getter_setter);
+
+our $verbose = 0;
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 8;
+}
+
+
+my $name = 'chromosome';
+my $version = 'NCBI33';
+my $dbID = 1;
+my $top_level = 1;
+my $sequence_level = 0;
+my $default = 1;
+
+#
+# Test constructor
+#
+my $coord_system = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => $name,
+ -VERSION => $version,
+ -DBID => $dbID,
+ -TOP_LEVEL => $top_level,
+ -SEQUENCE_LEVEL => $sequence_level,
+ -DEFAULT => 1);
+
+
+ok($coord_system && $coord_system->isa('Bio::EnsEMBL::CoordSystem'));
+
+
+#
+# Test name()
+#
+ok($coord_system->name() eq $name);
+
+#
+# Test version()
+#
+ok($coord_system->version() eq $version);
+
+#
+# Test is_top_level()
+#
+ok($coord_system->is_top_level());
+
+#
+# Test is_sequence_level()
+#
+ok(!$coord_system->is_sequence_level());
+
+#
+# Test is_default()
+#
+ok($coord_system->is_default());
+
+
+#
+# Test equals()
+#
+
+my $coord_system2 = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => $name,
+ -VERSION => $version,
+ -DBID => 123,
+ -TOP_LEVEL => $top_level);
+
+ok($coord_system->equals($coord_system2));
+
+$coord_system2 = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => 'chromosome',
+ -VERSION => 'NCBI34',
+ -DBID => 123,
+ -TOP_LEVEL => $top_level);
+
+ok(!$coord_system->equals($coord_system2));
+
+
+
+
diff --git a/modules/t/coordSystemAdaptor.t b/modules/t/coordSystemAdaptor.t
new file mode 100644
index 0000000000..f4722f7713
--- /dev/null
+++ b/modules/t/coordSystemAdaptor.t
@@ -0,0 +1,155 @@
+use lib 't';
+use strict;
+
+BEGIN { $| = 1;
+ use Test ;
+ plan tests => 25;
+}
+
+use MultiTestDB;
+use TestUtils qw(debug test_getter_setter);
+
+our $verbose = 0; #set to 1 to turn on debug printouts
+
+my $multi = MultiTestDB->new();
+my $db = $multi->get_DBAdaptor( 'core' );
+
+
+#
+# Test constructor
+#
+
+my $csa = $db->get_CoordSystemAdaptor();
+
+ok($csa && $csa->isa('Bio::EnsEMBL::DBSQL::CoordSystemAdaptor'));
+
+
+#
+# Test fetch_by_name()
+#
+
+my $cs = $csa->fetch_by_name('chromosome');
+
+ok($cs->name eq 'chromosome');
+ok($cs->dbID());
+ok($cs->version eq 'NCBI33');
+ok($cs->is_top_level());
+ok(!$cs->is_sequence_level());
+ok($cs->is_default());
+
+
+#
+# Test fetch_all_by_name
+#
+my @cs_list = @{$csa->fetch_all_by_name('chromosome')};
+
+ok(@cs_list == 1);
+
+ok($cs_list[0]->equals($cs));
+
+
+#
+# Test fetch_by_dbID()
+#
+$cs = $csa->fetch_by_dbID(3);
+
+ok($cs->name() eq 'clone');
+ok($cs->version() eq '');
+
+
+
+#
+# 11 Test fetch_top_level
+#
+$cs = $csa->fetch_top_level();
+
+ok($cs->name eq 'chromosome');
+
+#
+# 12 Test fetch_all_top_level
+#
+($cs) = @{$csa->fetch_all_top_level()};
+
+ok($cs->name eq 'chromosome');
+
+
+#
+# 13-14 Test fetch_sequence_level
+#
+$cs = $csa->fetch_sequence_level();
+
+ok($cs->name eq 'contig');
+ok($cs->is_sequence_level());
+
+
+#
+# 15-16 Test get_mapping_path
+#
+
+my $ctg_cs = $csa->fetch_by_name('contig');
+my $chr_cs = $csa->fetch_by_name('chromosome');
+my $cln_cs = $csa->fetch_by_name('clone');
+
+my $path = $csa->get_mapping_path($ctg_cs, $chr_cs);
+
+ok(@$path == 2 &&
+ $path->[0]->name() eq 'chromosome' &&
+ $path->[1]->name() eq 'contig');
+
+$path = $csa->get_mapping_path($chr_cs, $cln_cs);
+
+
+ok(@$path == 3 &&
+ (($path->[0]->name eq 'chromosome' && #there are 2 equally valid paths
+ $path->[1]->name eq 'contig' &&
+ $path->[2]->name eq 'clone')
+ ||
+ ($path->[0]->name eq 'clone' &&
+ $path->[1]->name eq 'contig' &&
+ $path->[2]->name eq 'chromosome'))
+ );
+
+#
+# Test store
+#
+
+$multi->save('core', 'coord_system');
+
+$cs = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => 'chromosome',
+ -VERSION => 'NCBI35',
+ -DEFAULT => 0,
+ -SEQUENCE_LEVEL => 0,
+ -TOP_LEVEL => 1);
+
+$csa->store($cs);
+
+ok($cs->adaptor == $csa);
+ok($cs->dbID());
+
+#now make sure we can retrieve this
+$cs = $csa->fetch_by_name('chromosome', 'NCBI35');
+ok($cs->name eq 'chromosome');
+ok($cs->version eq 'NCBI35');
+ok(!$cs->is_default);
+ok(!$cs->is_sequence_level);
+ok($cs->is_top_level);
+
+my $sth = $db->prepare('SELECT attrib FROM coord_system ' .
+ 'WHERE name = ? and version = ?');
+$sth->execute('chromosome', 'NCBI35');
+
+my ($attrib) = $sth->fetchrow_array();
+ok($attrib eq 'top_level');
+$sth->finish();
+
+$multi->restore('core', 'coord_system');
+
+
+
+
+
+
+
+
+
diff --git a/modules/t/dbConnection.t b/modules/t/dbConnection.t
index 9001c08c14..453684b6fc 100644
--- a/modules/t/dbConnection.t
+++ b/modules/t/dbConnection.t
@@ -91,8 +91,6 @@ ok(test_getter_setter($dbc, 'db_handle', $db->db_handle));
my $sth = $dbc->prepare('SELECT * from gene limit 1');
$sth->execute;
ok($sth->rows);
-$sth->finish();
-
#
# 12 add_db_adaptor
diff --git a/modules/t/dbEntries.t b/modules/t/dbEntries.t
index b5be1473e2..60eb5617d7 100644
--- a/modules/t/dbEntries.t
+++ b/modules/t/dbEntries.t
@@ -4,14 +4,13 @@ use warnings;
BEGIN { $| = 1;
use Test;
- plan tests => 14;
+ plan tests => 12;
}
use MultiTestDB;
use TestUtils qw ( debug test_getter_setter );
use Bio::EnsEMBL::DBEntry;
-use Bio::EnsEMBL::ProteinFeature;
# switch on the debug prints
@@ -102,33 +101,19 @@ my $goref = Bio::EnsEMBL::GoXref->new
);
$goref->add_linkage_type( "IC" );
-my $analysis = Bio::EnsEMBL::Analysis->new
- ( -logic_name => 'protmap',
- -program => 'pmatch',
- -database => 'pmatch' );
-
-
my $ident_xref = Bio::EnsEMBL::IdentityXref->new
(
-primary_id => "1",
-dbname => "SPTREMBL",
-release => "1",
- -display_id => "Ens related Ident",
- -cigar_line => "10MDI2M3D2M3I5M",
- -translation_start => 1,
- -translation_end => 23,
- -hit_start => 1,
- -hit_end => 23,
- -score => 20.0,
- -evalue => 123,
- -analysis => $analysis
+ -display_id => "Ens related Ident"
);
$ident_xref->query_identity( 100 );
$ident_xref->target_identity( 95 );
-$multi->hide( "core", "object_xref", "xref", "identity_xref", "go_xref", "analysis" );
+$multi->hide( "core", "object_xref", "xref", "identity_xref", "go_xref" );
my $gene = $ga->fetch_by_dbID( $all_gene_ids->[0] );
@@ -137,8 +122,6 @@ my $tl = $tr->translation();
-
-
$dbEntryAdaptor->store( $xref, $gene, "Gene" );
$dbEntryAdaptor->store( $xref, $tr, "Transcript" );
$dbEntryAdaptor->store( $goref, $tl, "Translation" );
@@ -200,44 +183,11 @@ debug( "Number of identity_xrefs = $ident_count" );
ok( $ident_count == 2 );
-my $protein_feature = Bio::EnsEMBL::ProteinFeature->new
- (
- -start => 1,
- -end => 23,
- -seqname => 'SP0001'
- );
-
-my $mapper = $ident_xref->get_mapper();
-
-debug( "Mapper ".ref( $mapper ) );
-
-my $copy_features = $ident_xref->transform_feature( $protein_feature );
-
-for my $feat ( @$copy_features ) {
- debug(join("\n", map({$_ ."->" . $feat->{$_} } keys %$feat) ));
- debug( "------------");
-}
-
-# 4 M-sections in the xref make 4 mapped sections
-
-#
-# 10 mapping features via alignment
-#
-ok( scalar( @$copy_features ) == 4 );
-
-# 10M is the first matching section
-
-#
-# 11 mapping first section to 1-10
-#
-ok( $copy_features->[0]->start() == 1 &&
- $copy_features->[0]->end() == 10 );
-
$multi->restore();
#
-# 12-14 Test that external synonyms and go evidence tags are retrieved
+# 10-12 Test that external synonyms and go evidence tags are retrieved
#
my $ta = $db->get_TranscriptAdaptor();
my $translation = $ta->fetch_by_dbID(21737)->translation;
diff --git a/modules/t/densityFeature.t b/modules/t/densityFeature.t
new file mode 100644
index 0000000000..680001c88f
--- /dev/null
+++ b/modules/t/densityFeature.t
@@ -0,0 +1,54 @@
+
+use lib 't';
+
+use strict;
+use warnings;
+
+use Bio::EnsEMBL::DensityFeature;
+use Bio::EnsEMBL::Analysis;
+
+use TestUtils qw(debug test_getter_setter);
+
+
+our $verbose = 0; #set to 1 to turn on debug printouts
+
+
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 6;
+}
+
+use TestUtils qw( debug );
+
+my $analysis = Bio::EnsEMBL::Analysis->new(-DBID => 1,
+ -LOGIC_NAME => 'test');
+
+
+my $start = 10;
+my $end = 102;
+my $density_value = 123;
+
+my $df = Bio::EnsEMBL::DensityFeature->new
+ (-start => $start,
+ -end => $end,
+ -analysis => $analysis,
+ -density_value => $density_value);
+
+ok($df->start == $start && $df->analysis == $analysis && $df->end == $end);
+ok($df->strand == 0);
+
+ok($df->density_value == $density_value);
+
+$df = Bio::EnsEMBL::DensityFeature->new_fast
+ ({'start' => $start,
+ 'end' => $end,
+ 'analysis' => $analysis,
+ 'density_value' => $density_value});
+
+ok($df->start == $start && $df->analysis == $analysis && $df->end == $end);
+ok($df->strand == 0);
+ok($df->density_value == $density_value);
+
+
+
diff --git a/modules/t/densityFeatureAdaptor.t b/modules/t/densityFeatureAdaptor.t
new file mode 100644
index 0000000000..52dac028b4
--- /dev/null
+++ b/modules/t/densityFeatureAdaptor.t
@@ -0,0 +1,105 @@
+
+use strict;
+use warnings;
+
+use Bio::EnsEMBL::Utils::Exception qw(verbose);
+use TestUtils qw( debug );
+use MultiTestDB;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 11;
+}
+
+
+
+our $verbose = 0;
+verbose('WARNING');
+
+my $multi_db = MultiTestDB->new;
+my $db = $multi_db->get_DBAdaptor('core');
+
+
+my $dfa = $db->get_DensityFeatureAdaptor();
+
+ok(ref($dfa) && $dfa->isa('Bio::EnsEMBL::DBSQL::DensityFeatureAdaptor'));
+
+my $slice_adaptor = $db->get_SliceAdaptor();
+my $slice = $slice_adaptor->fetch_by_region('chromosome', '20', 1, 600);
+
+my $dfs = $dfa->fetch_all_by_Slice($slice, 'SNPDensity');
+ok(@$dfs);
+print_features($dfs);
+$dfs = $dfa->fetch_all_by_Slice($slice, 'RepeatCoverage');
+ok(@$dfs);
+print_features($dfs);
+
+
+$dfs = $dfa->fetch_all_by_Slice($slice, 'SNPDensity', 10, 1);
+ok(@$dfs);
+print_features($dfs);
+$dfs = $dfa->fetch_all_by_Slice($slice, 'RepeatCoverage', 10, 1);
+ok(@$dfs);
+print_features($dfs);
+
+$dfs = $dfa->fetch_all_by_Slice($slice, 'SNPDensity', 50, 1);
+ok(@$dfs);
+print_features($dfs);
+$dfs = $dfa->fetch_all_by_Slice($slice, 'RepeatCoverage', 50, 1);
+ok(@$dfs);
+print_features($dfs);
+
+$dfs = $dfa->fetch_all_by_Slice($slice, 'SNPDensity', 2, 1);
+ok(@$dfs);
+print_features($dfs);
+$dfs = $dfa->fetch_all_by_Slice($slice, 'RepeatCoverage', 2, 1);
+ok(@$dfs);
+print_features($dfs);
+
+
+
+$slice_adaptor->fetch_by_region('chromosome', '20', 50, 600);
+
+$dfs = $dfa->fetch_all_by_Slice($slice, 'SNPDensity', 50, 1);
+ok(@$dfs);
+print_features($dfs);
+$dfs = $dfa->fetch_all_by_Slice($slice, 'RepeatCoverage', 50, 1);
+ok(@$dfs);
+print_features($dfs);
+
+
+
+#
+# helper to draw an ascii representation of the density features
+#
+sub print_features {
+ my $features = shift;
+
+ return if(!@$features);
+
+ my $sum = 0;
+ my $length = 0;
+ my $type = $features->[0]->density_value_type();
+
+ debug("\n");
+ foreach my $f (@$features) {
+ my $draw_width = 1;
+ my $density_value = $f->density_value();
+ my $draw_height = int(0.75 * $density_value);
+ $sum += $density_value;
+ $length += $f->length();
+ for(my $i = 0; $i < $draw_width; $i++) {
+ debug(('*'x$draw_height) . "($density_value)");
+ }
+ }
+ my $avg = undef;
+ $avg = $sum/$length if($length < 0);
+ debug("Type=$type, Sum=$sum, Length=$length, Avg/Base=$sum");
+}
+
+
+
+
+
diff --git a/modules/t/dnaAlignFeatureAdaptor.t b/modules/t/dnaAlignFeatureAdaptor.t
index f4100e0a52..293e054c24 100644
--- a/modules/t/dnaAlignFeatureAdaptor.t
+++ b/modules/t/dnaAlignFeatureAdaptor.t
@@ -1,12 +1,15 @@
use lib 't';
+use strict;
BEGIN { $| = 1;
use Test;
- plan tests => 2;
+ plan tests => 23;
}
use MultiTestDB;
+
+use Bio::EnsEMBL::DnaDnaAlignFeature;
use TestUtils qw(test_getter_setter debug);
our $verbose = 0;
@@ -22,3 +25,138 @@ debug("DnaAlignFeatureAdaptor->list_dbIDs");
my $ids = $dafa->list_dbIDs();
ok (@{$ids});
+
+#
+# Test retrieval via Slice
+#
+
+my $feats;
+
+my $chr_slice = $db->get_SliceAdaptor->fetch_by_region('chromosome','20',
+ 30_500_000, 30_700_000);
+
+$feats = $dafa->fetch_all_by_Slice($chr_slice);
+debug('---fetching by chromosomal slice---');
+debug("Got " . scalar(@$feats) . " features back");
+ok(@$feats == 6188);
+print_features($feats);
+
+
+my $ctg_slice;
+$ctg_slice = $db->get_SliceAdaptor->fetch_by_region('contig',
+ 'AL031658.11.1.162976',
+ 1,
+ 50_000);
+$feats = $dafa->fetch_all_by_Slice($ctg_slice);
+debug('--- contig AL031658.11.1.162976 (1-50000) features ---');
+debug("Got " . scalar(@$feats));
+ok(@$feats == 709);
+print_features($feats);
+
+
+#
+# Test fetch_by_dbID
+#
+my $feat = $dafa->fetch_by_dbID(22171863);
+debug('--- fetching by dbID ---');
+ok($feat);
+print_features([$feat]);
+
+
+$feat = $feat->transform('supercontig');
+debug('--- transform to supercontig coords ---');
+ok($feat);
+print_features([$feat]);
+
+
+#
+# Test fetch_by_Slice_and_pid
+#
+$feats = $dafa->fetch_all_by_Slice_and_pid($chr_slice, '90');
+debug('--- fetching by chr Slice and pid (90) ---');
+debug("Got " . scalar(@$feats));
+ok(@$feats == 452);
+#print_features($feats);
+
+#
+# Test store
+#
+
+$multi->save('core', 'dna_align_feature');
+
+my $analysis = $feat->analysis;
+my $slice =
+ $db->get_SliceAdaptor->fetch_by_region('contig','AL031658.11.1.162976');
+my $start = 100;
+my $end = 200;
+my $strand = 1;
+my $hstart = 10;
+my $hend = 110;
+my $hstrand = -1;
+my $hseqname = 'test';
+my $score = 80;
+my $percent_id = 90;
+my $evalue = 23.2;
+my $cigar_string = '100M';
+
+$feat = Bio::EnsEMBL::DnaDnaAlignFeature->new
+ (-START => $start,
+ -END => $end,
+ -STRAND => $strand,
+ -SLICE => $slice,
+ -HSTART => $hstart,
+ -HEND => $hend,
+ -HSTRAND => $hstrand,
+ -HSEQNAME => $hseqname,
+ -CIGAR_STRING => '100M',
+ -PERCENT_ID => $percent_id,
+ -SCORE => $score,
+ -P_VALUE => $evalue,
+ -ANALYSIS => $analysis);
+
+ok(!$feat->is_stored($db));
+
+$dafa->store($feat);
+
+ok($feat->is_stored($db));
+
+my $dbID = $feat->dbID();
+
+$feat = $dafa->fetch_by_dbID($dbID, 'contig');
+
+ok($feat->dbID == $dbID);
+ok($feat->start == $start);
+ok($feat->end == $end);
+ok($feat->strand == $strand);
+ok($feat->slice->name eq $slice->name);
+ok($feat->hstart == $hstart);
+ok($feat->hend == $hend);
+ok($feat->hstrand == $hstrand);
+ok($feat->hseqname eq $hseqname);
+ok($feat->cigar_string eq $cigar_string);
+ok($feat->percent_id == $percent_id);
+ok($feat->score == $score);
+ok($feat->p_value == $evalue);
+ok($feat->analysis->logic_name eq $analysis->logic_name);
+
+$multi->restore('core', 'dna_align_feature');
+
+
+
+
+sub print_features {
+ return if(!$verbose);
+ my $features = shift;
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ my $seqname = $f->slice->seq_region_name();
+ my $analysis = $f->analysis->logic_name();
+ debug($seqname . ' ' . $f->start().'-'.$f->end().'('.$f->strand().
+ ') ['. $f->dbID.'] '. $f->cigar_string . ' ' .
+ $f->hstart .'-'.$f->hend.' ('.$f->hstrand.')'.$f->score() .
+ " ($analysis) " . $f->percent_id);
+ } else {
+ debug('UNDEF');
+ }
+ }
+}
diff --git a/modules/t/dnaDnaAlignFeature.t b/modules/t/dnaDnaAlignFeature.t
index cec4c3be04..64ea7709d8 100644
--- a/modules/t/dnaDnaAlignFeature.t
+++ b/modules/t/dnaDnaAlignFeature.t
@@ -3,13 +3,14 @@ use lib 't';
BEGIN { $| = 1;
use Test;
- plan tests => 63;
+ plan tests => 14;
}
+use strict;
+
use MultiTestDB;
use Bio::EnsEMBL::DnaDnaAlignFeature;
-use Bio::EnsEMBL::SeqFeature;
-use Bio::EnsEMBL::RawContig;
+use Bio::EnsEMBL::FeaturePair;
my($CHR, $START, $END) = ('20', 30_363_615, 30_475_000);
@@ -25,63 +26,52 @@ my $db = $multi_db->get_DBAdaptor('core');
-my $slice = $db->get_SliceAdaptor->fetch_by_chr_start_end($CHR,$START,$END);
+my $chr_slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ $CHR,$START,$END);
+
+my $ctg_slice = $db->get_SliceAdaptor->fetch_by_region('contig',
+ 'AL359765.6.1.13780');
-my $contig = new Bio::EnsEMBL::RawContig;
-$contig->seq('ACTGACTG');
-$contig->name('bogus contig');
my @feats;
-my $fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start(5);
-$fp->end (7);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($contig);
-$fp->hstart(105);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-
-push(@feats,$fp);
-
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start(10);
-$fp->end (14);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($contig);
-$fp->seqname(1);
-
-$fp->hstart (108);
-$fp->hend (112);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
+push @feats, Bio::EnsEMBL::FeaturePair->new
+ (-START => 5,
+ -END => 7,
+ -STRAND => 1,
+ -SCORE => 10,
+ -SLICE => $ctg_slice,
+ -HSTART => 105,
+ -HEND => 107,
+ -HSTRAND => 1,
+ -HSEQNAME => 'dummy-hid');
-#
-#
-# 2 Test DnaDnaAlignFeature::new(-features)
-#
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf && $dnaf->validate);
+push @feats, Bio::EnsEMBL::FeaturePair->new
+ (-start => 10,
+ -end => 14,
+ -strand => 1,
+ -score => 10,
+ -slice => $ctg_slice,
+ -hstart => 108,
+ -hend => 112,
+ -hstrand => 1,
+ -hseqname => 'dummy-hid');
#
-# 3 Test DnaDnaAlignFeature::seqname
#
-ok($dnaf->seqname eq 'bogus contig');
+# Test DnaDnaAlignFeature::new(-features)
+#
+my $dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+ok(ref($dnaf) && $dnaf->isa('Bio::EnsEMBL::DnaDnaAlignFeature'));
#
-# 4 Test DnaDnaAlignFeature::hseqname
+# Test DnaDnaAlignFeature::hseqname
#
ok($dnaf->hseqname eq 'dummy-hid');
-
#
# 5 Test DnaDnaAlignFeature::cigar_string
#
-ok($dnaf->cigar_string =~ '3M2I5M');
+ok($dnaf->cigar_string eq '3M2I5M');
#
# 6-8 Test DnaDnaAlignFeature::reverse_complement
@@ -89,9 +79,9 @@ ok($dnaf->cigar_string =~ '3M2I5M');
my $strand = $dnaf->strand;
my $hstrand = $dnaf->hstrand;
$dnaf->reverse_complement;
-ok($dnaf->cigar_string =~ '5M2I3M');
+ok($dnaf->cigar_string eq '5M2I3M');
ok(($strand*-1) == $dnaf->strand);
-ok(($hstrand*-1) == $dnaf->hstrand);
+ok(($hstrand*-1) == $dnaf->hstrand);
@@ -114,7 +104,7 @@ ok( scalar($dnaf->ungapped_features) == 2);
#
# 12 Test retrieval from database
#
-my $features = $slice->get_all_DnaAlignFeatures;
+my $features = $chr_slice->get_all_DnaAlignFeatures;
ok(scalar @$features);
@@ -122,469 +112,469 @@ ok(scalar @$features);
# 13 Test transformation to raw contig
#
my $f = $features->[0];
-my @fs = $f->transform;
+my @fs = $f->transform('contig');
ok( scalar @fs );
#
# 14 Test transformation back to slice
#
($f) = @fs;
-$f = $f->transform($slice);
+$f = $f->transfer($chr_slice);
ok($f);
#
# 15 Test transformation onto negative strand slice
#
-$f = $f->transform($slice->invert);
+$f = $f->transfer($chr_slice->invert);
ok($f);
-#
-# 16-21 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (+ve strand, +ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 2);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(105);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 3);
-$fp->end ($CTG_BOUNDARY + 7);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (108);
-$fp->hend (112);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 12);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (115);
-$fp->hend (119);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-#
-# 22-27 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (+ve strand, -ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 2);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (108);
-$fp->hend (110);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 3);
-$fp->end ($CTG_BOUNDARY + 7);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart(103);
-$fp->hend (107);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 12);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (96);
-$fp->hend (100);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-my @dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-#
-# 28-33 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (-ve strand, +ve hitstrand)
-#
-@feats = ();
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 10);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (100);
-$fp->hend (102);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 1);
-$fp->end ($CTG_BOUNDARY + 5);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(103);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 4);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart (110);
-$fp->hend (114);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-
-#
-# 34-39 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (-ve strand, -ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 3);
-$fp->end ($CTG_BOUNDARY + 5);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (108);
-$fp->hend (110);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 4);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart(103);
-$fp->hend (107);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 9);
-$fp->end ($CTG_BOUNDARY - 5);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart(96);
-$fp->hend(100);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-#
-#
-# Do the same tests again on the negative strand slice
-#
-#
-$CTG_BOUNDARY = $slice->length - $CTG_BOUNDARY + 1;
-$slice = $slice->invert;
-
-#
-# 40-45 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (+ve strand, +ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 2);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(105);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 3);
-$fp->end ($CTG_BOUNDARY + 7);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (108);
-$fp->hend (112);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 12);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (115);
-$fp->hend (119);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-#
-# 46-51 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (+ve strand, -ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 2);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (108);
-$fp->hend (110);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 3);
-$fp->end ($CTG_BOUNDARY + 7);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart(103);
-$fp->hend (107);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 12);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (96);
-$fp->hend (100);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-#
-# 52-57 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (-ve strand, +ve hitstrand)
-#
-@feats = ();
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 10);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (100);
-$fp->hend (102);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 1);
-$fp->end ($CTG_BOUNDARY + 5);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(103);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 4);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart (110);
-$fp->hend (114);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-
-#
-# 58-63 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (-ve strand, -ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 3);
-$fp->end ($CTG_BOUNDARY + 5);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (108);
-$fp->hend (110);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 4);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart(103);
-$fp->hend (107);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 9);
-$fp->end ($CTG_BOUNDARY - 5);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart(96);
-$fp->hend(100);
-$fp->hstrand (-1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M2I5M2D5M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
+##
+## 16-21 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (+ve strand, +ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 2);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(105);
+#$fp->hend (107);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 3);
+#$fp->end ($CTG_BOUNDARY + 7);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (108);
+#$fp->hend (112);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 12);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (115);
+#$fp->hend (119);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+##
+## 22-27 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (+ve strand, -ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 2);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (108);
+#$fp->hend (110);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 3);
+#$fp->end ($CTG_BOUNDARY + 7);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart(103);
+#$fp->hend (107);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 12);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (96);
+#$fp->hend (100);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#my @dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+##
+## 28-33 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (-ve strand, +ve hitstrand)
+##
+#@feats = ();
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 10);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (100);
+#$fp->hend (102);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 1);
+#$fp->end ($CTG_BOUNDARY + 5);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(103);
+#$fp->hend (107);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 4);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart (110);
+#$fp->hend (114);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+
+##
+## 34-39 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (-ve strand, -ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 3);
+#$fp->end ($CTG_BOUNDARY + 5);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (108);
+#$fp->hend (110);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 4);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart(103);
+#$fp->hend (107);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 9);
+#$fp->end ($CTG_BOUNDARY - 5);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart(96);
+#$fp->hend(100);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+##
+##
+## Do the same tests again on the negative strand slice
+##
+##
+#$CTG_BOUNDARY = $slice->length - $CTG_BOUNDARY + 1;
+#$slice = $slice->invert;
+
+##
+## 40-45 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (+ve strand, +ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 2);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(105);
+#$fp->hend (107);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 3);
+#$fp->end ($CTG_BOUNDARY + 7);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (108);
+#$fp->hend (112);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 12);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (115);
+#$fp->hend (119);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+##
+## 46-51 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (+ve strand, -ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 2);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (108);
+#$fp->hend (110);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 3);
+#$fp->end ($CTG_BOUNDARY + 7);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart(103);
+#$fp->hend (107);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 12);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (96);
+#$fp->hend (100);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+##
+## 52-57 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (-ve strand, +ve hitstrand)
+##
+#@feats = ();
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 10);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (100);
+#$fp->hend (102);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 1);
+#$fp->end ($CTG_BOUNDARY + 5);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(103);
+#$fp->hend (107);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 4);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart (110);
+#$fp->hend (114);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+
+##
+## 58-63 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (-ve strand, -ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 3);
+#$fp->end ($CTG_BOUNDARY + 5);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (108);
+#$fp->hend (110);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 4);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart(103);
+#$fp->hend (107);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 9);
+#$fp->end ($CTG_BOUNDARY - 5);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart(96);
+#$fp->hend(100);
+#$fp->hstrand (-1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaDnaAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M2I5M2D5M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
diff --git a/modules/t/dnaPepAlignFeature.t b/modules/t/dnaPepAlignFeature.t
index b1d1ceb73b..7ff1a35370 100644
--- a/modules/t/dnaPepAlignFeature.t
+++ b/modules/t/dnaPepAlignFeature.t
@@ -1,15 +1,15 @@
use lib 't';
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test;
- plan tests => 39;
+ plan tests => 14;
}
+use strict;
+
use MultiTestDB;
use Bio::EnsEMBL::DnaPepAlignFeature;
-use Bio::EnsEMBL::SeqFeature;
-use Bio::EnsEMBL::RawContig;
use TestUtils qw ( debug test_getter_setter );
@@ -30,63 +30,57 @@ my $db = $multi_db->get_DBAdaptor('core');
-my $slice = $db->get_SliceAdaptor->fetch_by_chr_start_end($CHR,$START,$END);
+my $chr_slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ $CHR,$START,$END);
-my $contig = new Bio::EnsEMBL::RawContig;
-$contig->seq('ACTGACTG');
-$contig->name('bogus contig');
+my $ctg_slice = $db->get_SliceAdaptor->fetch_by_region('contig',
+ 'AL359765.6.1.13780');
my @feats;
-my $fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start(5);
-$fp->end (7);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($contig);
-$fp->hstart(105);
-$fp->hend (105);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start(11);
-$fp->end (16);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($contig);
-$fp->hstart (106);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-#
-#
-# 2 Test DnaPepAlignFeature::new(-features)
-#
-$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
-ok($dnaf && $dnaf->validate);
+push @feats, Bio::EnsEMBL::FeaturePair->new
+ (-start => 5,
+ -end => 7,
+ -score => 10,
+ -strand => 1,
+ -slice => $ctg_slice,
+ -hstart => 105,
+ -hend => 105,
+ -hstrand => 1,
+ -hseqname => 'dummy-hid');
+
+push @feats, Bio::EnsEMBL::FeaturePair->new
+ (-start => 11,
+ -end => 16,
+ -score => 10,
+ -strand => 1,
+ -slice => $ctg_slice,
+ -hstart => 106,
+ -hend => 107,
+ -hstrand => 1,
+ -hseqname => 'dummy-hid');
+
+#
#
-# 3 Test DnaPepAlignFeature::seqname
+# Test DnaPepAlignFeature::new(-features)
#
-ok($dnaf->seqname eq 'bogus contig');
+my $dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
+ok(ref($dnaf) && $dnaf->isa('Bio::EnsEMBL::DnaPepAlignFeature'));
#
-# 4 Test DnaPepAlignFeature::hseqname
+# Test DnaPepAlignFeature::hseqname
#
ok($dnaf->hseqname eq 'dummy-hid');
#
-# 5 Test DnaPepAlignFeature::cigar_string
+# Test DnaPepAlignFeature::cigar_string
#
ok($dnaf->cigar_string =~ '3M3I6M');
#
-# 6-8 Test DnaPepAlignFeature::reverse_complement
+# Test DnaPepAlignFeature::reverse_complement
#
my $strand = $dnaf->strand;
my $hstrand = $dnaf->hstrand;
@@ -98,17 +92,17 @@ ok(($hstrand*-1) == $dnaf->hstrand);
#
-# 9 Test DnaPepAlignFeature::start
+# Test DnaPepAlignFeature::start
#
ok($dnaf->start == 5);
#
-# 10 Test DnaPepAlignFeature::end
+# Test DnaPepAlignFeature::end
#
ok($dnaf->end == 16);
#
-# 11 Test DnaPepAlignFeature::ungapped_features
+# Test DnaPepAlignFeature::ungapped_features
#
ok( scalar($dnaf->ungapped_features) == 2);
@@ -116,273 +110,273 @@ ok( scalar($dnaf->ungapped_features) == 2);
#
# 12 Test retrieval from database
#
-my $features = $slice->get_all_ProteinAlignFeatures;
+my $features = $chr_slice->get_all_ProteinAlignFeatures;
ok(scalar @$features);
#
-# 13 Test transformation to raw contig
+# Test transformation to raw contig
#
my $f = $features->[0];
-my @fs = $f->transform;
+my @fs = $f->transform('contig');
ok( scalar @fs );
#
-# 14 Test transformation back to slice
+# Test transformation back to slice
#
($f) = @fs;
-$f = $f->transform($slice);
+$f = $f->transfer($chr_slice);
ok($f);
#
-# 15 Test transformation onto negative strand slice
+# Test transformation onto negative strand slice
#
-$f = $f->transform($slice->invert);
+$f = $f->transfer($chr_slice->invert);
ok($f);
-#
-# 16-21 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (+ve strand, +ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 5);
-$fp->end ($CTG_BOUNDARY );
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(104);
-$fp->hend (105);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 4);
-$fp->end ($CTG_BOUNDARY + 9);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (106);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 10);
-$fp->end ($CTG_BOUNDARY + 12);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (110);
-$fp->hend (110);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '6M3I6M6D3M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-
-
-#
-# 22-27 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (-ve strand, +ve hitstrand)
-#
-@feats = ();
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 10);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (100);
-$fp->hend (100);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 1);
-$fp->end ($CTG_BOUNDARY + 4);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(101);
-$fp->hend (102);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 4);
-$fp->end ($CTG_BOUNDARY - 2);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart (105);
-$fp->hend (105);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M3I6M6D3M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-
-debug( "Feature 0 dump" );
-while( my ($k, $v) = each %{$dnafs[0]} ) {
- debug( " ->".$k." = ".$v );
-}
-
-ok($dnafs[0]->validate || 1);
-
-debug( "Feature 1 dump" );
-while( my ($k, $v) = each %{$dnafs[1]} ) {
- debug( " ->".$k." = ".$v );
-}
-ok($dnafs[1]->validate || 1);
-
-
-
-
-#
-#
-# Do the same tests again on the negative strand slice
-#
-#
-$CTG_BOUNDARY = $slice->length - $CTG_BOUNDARY + 1;
-$slice = $slice->invert;
-
-
-
-#
-# 28-33 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (+ve strand, +ve hitstrand)
-#
-@feats = ();
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 2);
-$fp->end ($CTG_BOUNDARY);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(105);
-$fp->hend (105);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 4);
-$fp->end ($CTG_BOUNDARY + 9);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (106);
-$fp->hend (107);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 10);
-$fp->end ($CTG_BOUNDARY + 12);
-$fp->strand(1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (110);
-$fp->hend (110);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M3I6M6D3M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
-
-
-
-#
-# 34-39 create a dnaalign feature on a slice across a contig boundary
-# and convert to raw contig coordinates
-# (-ve strand, +ve hitstrand)
-#
-@feats = ();
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY + 8);
-$fp->end ($CTG_BOUNDARY + 10);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart (100);
-$fp->hend (100);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 1);
-$fp->end ($CTG_BOUNDARY + 4);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->hstart(101);
-$fp->hend (102);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-$fp = new Bio::EnsEMBL::FeaturePair;
-$fp->start($CTG_BOUNDARY - 4);
-$fp->end ($CTG_BOUNDARY - 2);
-$fp->strand(-1);
-$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
-$fp->hstart (105);
-$fp->hend (105);
-$fp->hstrand (1);
-$fp->hseqname('dummy-hid');
-push(@feats,$fp);
-
-
-$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
-ok($dnaf);
-ok($dnaf->cigar_string eq '3M3I6M6D3M');
-ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
-
-@dnafs = $dnaf->transform;
-ok(scalar(@dnafs) == 2);
-ok($dnafs[0]->validate || 1);
-ok($dnafs[1]->validate || 1);
+##
+## 16-21 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (+ve strand, +ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 5);
+#$fp->end ($CTG_BOUNDARY );
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(104);
+#$fp->hend (105);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 4);
+#$fp->end ($CTG_BOUNDARY + 9);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (106);
+#$fp->hend (107);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 10);
+#$fp->end ($CTG_BOUNDARY + 12);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (110);
+#$fp->hend (110);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '6M3I6M6D3M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+
+
+##
+## 22-27 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (-ve strand, +ve hitstrand)
+##
+#@feats = ();
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 10);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (100);
+#$fp->hend (100);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 1);
+#$fp->end ($CTG_BOUNDARY + 4);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(101);
+#$fp->hend (102);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 4);
+#$fp->end ($CTG_BOUNDARY - 2);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart (105);
+#$fp->hend (105);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M3I6M6D3M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+
+#debug( "Feature 0 dump" );
+#while( my ($k, $v) = each %{$dnafs[0]} ) {
+# debug( " ->".$k." = ".$v );
+#}
+
+#ok($dnafs[0]->validate || 1);
+
+#debug( "Feature 1 dump" );
+#while( my ($k, $v) = each %{$dnafs[1]} ) {
+# debug( " ->".$k." = ".$v );
+#}
+#ok($dnafs[1]->validate || 1);
+
+
+
+
+##
+##
+## Do the same tests again on the negative strand slice
+##
+##
+#$CTG_BOUNDARY = $slice->length - $CTG_BOUNDARY + 1;
+#$slice = $slice->invert;
+
+
+
+##
+## 28-33 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (+ve strand, +ve hitstrand)
+##
+#@feats = ();
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 2);
+#$fp->end ($CTG_BOUNDARY);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(105);
+#$fp->hend (105);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 4);
+#$fp->end ($CTG_BOUNDARY + 9);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (106);
+#$fp->hend (107);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 10);
+#$fp->end ($CTG_BOUNDARY + 12);
+#$fp->strand(1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (110);
+#$fp->hend (110);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M3I6M6D3M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
+
+
+
+##
+## 34-39 create a dnaalign feature on a slice across a contig boundary
+## and convert to raw contig coordinates
+## (-ve strand, +ve hitstrand)
+##
+#@feats = ();
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY + 8);
+#$fp->end ($CTG_BOUNDARY + 10);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart (100);
+#$fp->hend (100);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 1);
+#$fp->end ($CTG_BOUNDARY + 4);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->hstart(101);
+#$fp->hend (102);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+#$fp = new Bio::EnsEMBL::FeaturePair;
+#$fp->start($CTG_BOUNDARY - 4);
+#$fp->end ($CTG_BOUNDARY - 2);
+#$fp->strand(-1);
+#$fp->score(10);
+#$fp->contig($slice);
+#$fp->seqname(1);
+#$fp->hstart (105);
+#$fp->hend (105);
+#$fp->hstrand (1);
+#$fp->hseqname('dummy-hid');
+#push(@feats,$fp);
+
+
+#$dnaf = Bio::EnsEMBL::DnaPepAlignFeature->new( -features => \@feats );
+#ok($dnaf);
+#ok($dnaf->cigar_string eq '3M3I6M6D3M');
+#ok($dnaf->validate || 1); #validate doesn't return true but throws on fail
+
+#@dnafs = $dnaf->transform;
+#ok(scalar(@dnafs) == 2);
+#ok($dnafs[0]->validate || 1);
+#ok($dnafs[1]->validate || 1);
diff --git a/modules/t/exception.t b/modules/t/exception.t
new file mode 100644
index 0000000000..373b558fd2
--- /dev/null
+++ b/modules/t/exception.t
@@ -0,0 +1,86 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 9;
+}
+
+use TestUtils qw( debug );
+
+use Bio::EnsEMBL::Utils::Exception qw(warning verbose throw info
+ deprecate stack_trace_dump stack_trace);
+
+our $verbose= 0;
+
+
+if(!$verbose) {
+ verbose('NONE');
+}
+
+#
+#1 - test throw
+#
+
+eval {
+ throw('test exception');
+};
+
+ok($@);
+
+debug($@);
+
+#
+#2-4 - Test verbosity, warnings
+#
+
+$verbose && verbose('EXCEPTION');
+
+ok(verbose() == 1000 || (!$verbose && verbose() == 0));
+
+warning('This warn should not appear');
+
+ok(1);
+
+warning('This warn should appear', 1000);
+
+ok(1);
+
+info("This info should not appear");
+
+ok(1);
+
+$verbose && verbose('ALL');
+
+info("This info should appear");
+
+ok(1);
+
+#
+# 5-6 Test stack trace
+#
+
+my $std = stack_trace_dump();
+ok($std =~ /[A-Z]/);
+
+debug(stack_trace_dump);
+
+ok(stack_trace());
+
+
+#
+# 7 Test deprecate
+#
+
+test_deprecate();
+
+ok(7);
+
+sub test_deprecate {
+ deprecate('This deprecate warning should appear');
+}
+
+verbose('DEPRECATE');
+
diff --git a/modules/t/exon.t b/modules/t/exon.t
index ad5717c71a..b358c2119e 100644
--- a/modules/t/exon.t
+++ b/modules/t/exon.t
@@ -1,9 +1,9 @@
use lib 't';
use strict;
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test ;
- plan tests => 20;
+ plan tests => 22;
}
my $loaded = 0;
@@ -27,18 +27,20 @@ ok($db);
# Exon specific tests
my $exonad = $db->get_ExonAdaptor();
-my $rca = $db->get_RawContigAdaptor();
+my $slice_adaptor = $db->get_SliceAdaptor();
-my $contig = $rca->fetch_by_dbID( 469270 );
+my $slice = $slice_adaptor->fetch_by_region('chromosome', '20',
+ 30_811_000,
+ 32_000_000);
ok($exonad);
my $exon = Bio::EnsEMBL::Exon->new();
-$exon->start(31_200);
+$exon->start(1000);
ok(&test_getter_setter($exon, 'start', 200));
-$exon->end(31_400);
+$exon->end(1400);
ok(&test_getter_setter($exon, 'end', 400));
$exon->strand(1);
@@ -47,26 +49,28 @@ ok(&test_getter_setter($exon, 'strand', -1));
$exon->phase(0);
ok(&test_getter_setter($exon, 'phase', -1));
-$exon->contig( $contig );
-ok(&test_getter_setter($exon, 'contig', $contig));
+$exon->slice( $slice );
+ok(&test_getter_setter($exon, 'slice', $slice));
# should try to store (!)
$exon->end_phase( -1 );
ok(&test_getter_setter($exon, 'end_phase', 1));
-
#
# find supporting evidence for the exon
#
my @evidence = ();
my @fs = ();
-push @fs, @{$db->get_DnaAlignFeatureAdaptor->fetch_all_by_RawContig($contig)};
-push @fs, @{$db->get_ProteinAlignFeatureAdaptor->fetch_all_by_RawContig($contig)};
+push @fs, @{$db->get_DnaAlignFeatureAdaptor->fetch_all_by_Slice($slice)};
+push @fs, @{$db->get_ProteinAlignFeatureAdaptor->fetch_all_by_Slice($slice)};
while(my $f = shift @fs) {
#debug("feature at: " . $f->start . "-" . $f->end);
next if $f->start > $exon->end || $f->end < $exon->start;
push(@evidence, $f);
+ # cheat it into storing it again
+ $f->dbID( undef );
+ $f->adaptor( undef );
}
my $count = scalar(@evidence);
@@ -78,7 +82,7 @@ $multi->hide( "core", "exon", "supporting_feature",
$exonad->store($exon);
-ok($exon->dbID() == 1 && $exon->adaptor == $exonad);
+ok($exon->dbID() && $exon->adaptor == $exonad);
# now test fetch_by_dbID
@@ -87,43 +91,62 @@ my $newexon = $exonad->fetch_by_dbID($exon->dbID);
ok($newexon);
-#
-# Test transform to empty Slice
-#
-my $slice = new Bio::EnsEMBL::Slice(-empty => 1,
- -adaptor => $db->get_SliceAdaptor);
-$exon = $newexon->transform($slice);
debug("exon chr start = " . $exon->start);
debug("exon chr end = " . $exon->end);
-debug("exon chr strand = " . $exon->strand);
-ok($exon->start == 30_961_059 && $exon->end == 30_961_259 && $exon->strand==1);
+debug("exon chr strand = " . $exon->strand);
+
+debug("newexon start = " . $newexon->start());
+debug("newexon end = " . $newexon->end());
+debug("newexon strand = " . $newexon->strand());
+
+ok($newexon->start == 30811999 &&
+ $newexon->end == 30812399 &&
+ $newexon->strand==1);
#
# Test transform to another slice
#
-$slice = $db->get_SliceAdaptor->fetch_by_chr_start_end($slice->chr_name,
- $exon->start - 10,
- $exon->end + 10);
-$exon = $exon->transform($slice);
-debug("exon chr start = " . $exon->start);
-debug("exon chr end = " . $exon->end);
-debug("exon chr strand = " . $exon->strand);
-ok($exon->start == 11 && $exon->end == 211 && $exon->strand==1);
+$slice = $exon->slice();
+$slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ $slice->seq_region_name,
+ $slice->start + $exon->start - 11,
+ $slice->start + $exon->end + 9);
+$exon = $exon->transfer($slice);
+debug("exon start = " . $exon->start);
+debug("exon end = " . $exon->end);
+debug("exon strand = " . $exon->strand);
+ok($exon->start == 11 && $exon->end == 411 && $exon->strand==1);
#
-# Test Transform back to Raw Contig
+# Test Transform to contig coord system
#
-$exon = $exon->transform;
-ok($exon->start == 31_200);
-ok($exon->end == 31_400);
+$exon = $exon->transform('contig');
+
+debug("exon start = " . $exon->start);
+debug("exon end = " . $exon->end);
+debug("exon strand = " . $exon->strand);
+debug("exon seq_region = " . $exon->slice->seq_region_name);
+
+ok($exon->start == 913);
+ok($exon->end == 1313);
ok($exon->strand == 1);
-ok($exon->contig->name eq $exon->contig->name);
+ok($exon->slice->seq_region_name eq 'AL034550.31.1.118873');
+
#regression test, supporting evidence was lost post transform before...
-ok(scalar(@{$exon->get_all_supporting_features} == $count));
+my $se_count = scalar(@{$exon->get_all_supporting_features});
+
+debug("Got $se_count supporting feature after transform");
+ok($se_count == $count);
+
+#make sure that supporting evidencewas stored correctly
+$se_count = scalar(@{$newexon->get_all_supporting_features});
+debug("Got $se_count from newly stored exon");
+ok($se_count == $count);
+
# list_ functions
debug ("Exon->list_dbIDs");
@@ -134,5 +157,13 @@ debug ("Exon->list_stable_ids");
my $stable_ids = $exonad->list_stable_ids();
ok (@{$stable_ids});
+#hashkey
+my $hashkey = $exon->hashkey();
+debug($hashkey);
+
+ok($hashkey eq $exon->slice->name . '-' . $exon->start . '-' .
+ $exon->end . '-' . $exon->strand . '-' . $exon->phase .
+ '-' . $exon->end_phase);
+
$multi->restore();
diff --git a/modules/t/externalFeatureAdaptor.t b/modules/t/externalFeatureAdaptor.t
new file mode 100644
index 0000000000..8df98199c5
--- /dev/null
+++ b/modules/t/externalFeatureAdaptor.t
@@ -0,0 +1,146 @@
+use strict;
+
+our $verbose = 0;
+
+my $multi = MultiTestDB->new;
+
+package ExternalFF1;
+ use Bio::EnsEMBL::External::ExternalFeatureAdaptor;
+ use MultiTestDB;
+ use Bio::EnsEMBL::Feature;
+
+ use vars qw(@ISA);
+ @ISA = qw(Bio::EnsEMBL::External::ExternalFeatureAdaptor);
+
+ sub coordinate_systems {
+ return ('CONTIG');
+ }
+
+ sub ensembl_db {
+ my $self = shift;
+ return $multi->get_DBAdaptor("core");
+ }
+
+ sub fetch_all_by_contig_name {
+ my $self = shift;
+ my $name = shift;
+ my $start = shift;
+ my $end = shift;
+
+ my @out;
+
+ push @out, Bio::EnsEMBL::Feature->new(-START => 10_000,
+ -END => 11_000,
+ -STRAND => 1);
+
+ return \@out;
+ }
+
+
+
+
+package ExternalFF2;
+ use Bio::EnsEMBL::External::ExternalFeatureAdaptor;
+ use MultiTestDB;
+ use Bio::EnsEMBL::Feature;
+
+ use vars qw(@ISA);
+ @ISA = qw(Bio::EnsEMBL::External::ExternalFeatureAdaptor);
+
+ sub coordinate_systems {
+ return ('CLONE');
+ }
+
+ sub ensembl_db {
+ my $self = shift;
+ return $multi->get_DBAdaptor("core");
+ }
+
+ sub fetch_all_by_clone_accession {
+ my $self = shift;
+ my $name = shift;
+ my $version = shift;
+ my $start = shift;
+ my $end = shift;
+
+ my @out;
+
+ push @out, Bio::EnsEMBL::Feature->new(-START => 10_000,
+ -END => 11_000,
+ -STRAND => 1);
+
+ return \@out;
+ }
+
+
+
+
+package Test;
+
+use lib 't';
+use TestUtils qw(test_getter_setter debug);
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 5;
+}
+
+
+my $xff = ExternalFF1->new();
+
+my $db = $multi->get_DBAdaptor('core');
+
+my $slice_adaptor= $db->get_SliceAdaptor();
+my $slice = $slice_adaptor->fetch_by_region('chromosome', '20', 30e6,35e6);
+
+my $feats = $xff->fetch_all_by_Slice($slice);
+
+foreach my $f (@$feats) {
+ my ($start, $end, $strand) = ($f->start, $f->end, $f->strand);
+ debug("F: $start-$end ($strand)\n");
+}
+
+ok(@$feats == 12);
+
+my $cln_slice = $slice_adaptor->fetch_by_region('clone', 'AL359765.6');
+
+$feats = $xff->fetch_all_by_Slice($cln_slice);
+
+foreach my $f (@$feats) {
+ my ($start, $end, $strand) = ($f->start, $f->end, $f->strand);
+ debug("F: $start-$end ($strand)\n");
+}
+
+ok(@$feats == 1);
+
+$xff = ExternalFF2->new();
+
+$feats = $xff->fetch_all_by_Slice($slice);
+
+
+foreach my $f (@$feats) {
+ my ($start, $end, $strand) = ($f->start, $f->end, $f->strand);
+ debug("F: $start-$end ($strand)\n");
+}
+
+ok(@$feats == 12);
+
+$feats = $xff->fetch_all_by_Slice($cln_slice);
+
+foreach my $f (@$feats) {
+ my ($start, $end, $strand) = ($f->start, $f->end, $f->strand);
+ debug("F: $start-$end ($strand)\n");
+}
+
+ok(@$feats == 1);
+
+$feats = $xff->fetch_all_by_supercontig_name('NT_028392');
+
+foreach my $f (@$feats) {
+ my ($start, $end, $strand) = ($f->start, $f->end, $f->strand);
+ debug("F: $start-$end ($strand)\n");
+}
+
+ok(@$feats == 12);
+
+$multi=undef;
diff --git a/modules/t/feature.t b/modules/t/feature.t
new file mode 100644
index 0000000000..852b823a98
--- /dev/null
+++ b/modules/t/feature.t
@@ -0,0 +1,364 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 75;
+}
+
+use TestUtils qw( debug test_getter_setter );
+
+use Bio::EnsEMBL::Feature;
+use Bio::EnsEMBL::Slice;
+use Bio::EnsEMBL::Analysis;
+use Bio::EnsEMBL::CoordSystem;
+use MultiTestDB;
+
+our $verbose= 0; #turn on or off debug statements
+
+
+my $multi_db = MultiTestDB->new;
+my $db = $multi_db->get_DBAdaptor('core');
+
+my $coord_system = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => 'chromosome',
+ -VERSION => 'NCBI34',
+ -DBID => 123,
+ -TOP_LEVEL => 1);
+
+my $analysis = Bio::EnsEMBL::Analysis->new(-LOGIC_NAME => 'test');
+
+my $slice = Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $coord_system,
+ -SEQ_REGION_NAME => 'X',
+ -START => 1_000_000,
+ -END => 2_000_000);
+
+#
+# Test new and getters
+#
+my $start = 10;
+my $end = 100;
+my $strand = -1;
+my $feature = Bio::EnsEMBL::Feature->new(-START => 10,
+ -END => 100,
+ -STRAND => -1,
+ -ANALYSIS => $analysis,
+ -SLICE => $slice);
+
+
+ok($feature && $feature->isa('Bio::EnsEMBL::Feature'));
+
+ok($feature->start == $start);
+ok($feature->end == $end);
+ok($feature->strand == $strand);
+ok($feature->analysis == $analysis);
+ok($feature->slice == $slice);
+
+#
+# Test setters
+#
+$analysis = Bio::EnsEMBL::Analysis->new(-LOGIC_NAME => 'new analysis');
+$slice = Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $coord_system,
+ -SEQ_REGION_NAME => 'Y',
+ -START => 1_000_000,
+ -END => 2_000_000);
+ok(&test_getter_setter($feature, 'start', 1000));
+ok(&test_getter_setter($feature, 'end', 2000));
+ok(&test_getter_setter($feature, 'strand', 1));
+ok(&test_getter_setter($feature, 'analysis', $analysis));
+ok(&test_getter_setter($feature, 'slice', $slice));
+
+
+#
+# Test length
+#
+
+ok($feature->length == ($end-$start+1));
+
+
+#
+# Test move
+#
+
+$feature->move(300, 500, 1);
+
+ok($feature->start == 300);
+ok($feature->end == 500);
+ok($feature->strand == 1);
+
+
+#################
+# Test transform
+#################
+
+$slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ '20',
+ 30_249_935,
+ 31_000_000);
+
+$feature->slice($slice);
+
+#
+# Test Transform chromosome -> contig
+#
+
+$feature = $feature->transform('contig');
+
+debug("\nchromosome -> contig");
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+ok($feature->start() == 400);
+ok($feature->end() == 600);
+ok($feature->strand() == 1);
+ok($feature->slice->seq_region_name() eq 'AL359765.6.1.13780');
+ok($feature->slice->coord_system->name() eq 'contig');
+
+
+#
+# Test Transform contig -> supercontig
+#
+
+$feature = $feature->transform('supercontig');
+
+debug("\ncontig -> supercontig");
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+ok($feature->start() == 658269);
+ok($feature->end() == 658469);
+ok($feature->strand() == 1);
+ok($feature->slice->seq_region_name() eq 'NT_028392');
+ok($feature->slice->coord_system->name() eq 'supercontig');
+
+
+#
+# Test Transform supercontig -> contig
+#
+
+$feature = $feature->transform('contig');
+debug("\nsupercontig -> contig");
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+ok($feature->start() == 400);
+ok($feature->end() == 600);
+ok($feature->strand() == 1);
+ok($feature->slice->seq_region_name() eq 'AL359765.6.1.13780');
+ok($feature->slice->coord_system->name() eq 'contig');
+
+
+#
+# Test Transform contig -> clone
+#
+
+$feature = $feature->transform('clone');
+debug("\ncontig -> clone");
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+ok($feature->start() == 400);
+ok($feature->end() == 600);
+ok($feature->strand() == 1);
+ok($feature->slice->seq_region_name() eq 'AL359765.6');
+ok($feature->slice->coord_system->name() eq 'clone');
+
+
+#
+# Test transform to into gap
+#
+$slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ '20',
+ 1_000_000,
+ 2_000_000);
+$feature->slice($slice);
+
+ok(!defined($feature->transform('contig')));
+
+
+
+###############
+# Test transfer
+###############
+$slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ '20',
+ 30_249_935,
+ 31_000_000);
+
+$feature->slice($slice);
+
+
+#
+# Transfer to expanded inverted chr slice
+#
+
+#get larger slice on other strand
+$slice = $slice->invert()->expand(100,100);
+
+$feature = $feature->transfer($slice);
+debug("\ntransfer to inverted chromosomal slice");
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+ok($feature->start() == 749567);
+ok($feature->end() == 749767);
+ok($feature->strand() == -1);
+
+
+#
+# Transfer to contig slice
+#
+
+$slice = $db->get_SliceAdaptor->fetch_by_region('contig',
+ 'AL359765.6.1.13780',
+ '30', '3000');
+
+$feature = $feature->transfer($slice);
+debug("\ntransfer to contig slice");
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+
+ok($feature->start() == 471);
+ok($feature->end() == 671);
+ok($feature->strand() == 1);
+
+
+#
+# Transfer to supercontig slice
+#
+$slice = $db->get_SliceAdaptor->fetch_by_region('supercontig',
+ 'NT_028392');
+debug( "----------------------------" );
+
+$feature = $feature->transfer($slice);
+debug("\ntransfer to supercontig slice");
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+ok($feature->start() == 658369);
+ok($feature->end() == 658569);
+ok($feature->strand() == 1);
+
+
+#
+# Project over a contig boundary
+#
+
+$feature->move(671600,671800);
+
+debug( "Before projection to contig" );
+debug("start = " . $feature->start());
+debug("end = " . $feature->end());
+debug("strand = " . $feature->strand());
+debug("seq_region = " . $feature->slice->seq_region_name());
+
+my @projection = @{$feature->project('contig')};
+
+debug( "After project to contig" );
+foreach my $segment (@projection) {
+ ($start, $end, $slice) = @$segment;
+ debug("[$start-$end] -> " . $slice->seq_region_name
+ . ' ' . $slice->start . '-' . $slice->end . '('.$slice->strand().')');
+}
+debug( "-----------------------" );
+
+ok(@projection == 2);
+
+my ($seg1, $seg2) = @projection;
+
+ok($seg1 && $seg1->[0] == 1);
+ok($seg1 && $seg1->[1] == 50);
+ok($seg1 && $seg1->[2]->start == 13731);
+ok($seg1 && $seg1->[2]->end == 13780);
+ok($seg1 && $seg1->[2]->seq_region_name eq 'AL359765.6.1.13780');
+
+ok($seg2 && $seg2->[0] == 51);
+ok($seg2 && $seg2->[1] == 201);
+ok($seg2 && $seg2->[2]->start == 101);
+ok($seg2 && $seg2->[2]->end == 251);
+ok($seg2 && $seg2->[2]->seq_region_name eq 'AL031658.11.1.162976');
+
+debug('forward strand slice feature projection');
+foreach my $segment (@projection) {
+ ($start, $end, $slice) = @$segment;
+ debug("[$start-$end] -> " . $slice->seq_region_name
+ . ' ' . $slice->start . '-' . $slice->end . '('.$slice->strand().')');
+}
+
+#
+# Try a projection using an inverted slice
+#
+
+$slice = $feature->slice()->invert();
+$feature->slice($slice);
+$feature->move(4_391_950, 4_392_150);
+
+@projection = @{$feature->project('contig')};
+
+ok(@projection == 2);
+
+($seg1, $seg2) = @projection;
+
+ok($seg1 && $seg1->[0] == 1 );
+ok($seg1 && $seg1->[1] == 8 );
+ok($seg1 && $seg1->[2]->start == 101);
+ok($seg1 && $seg1->[2]->end == 108);
+ok($seg1 && $seg1->[2]->seq_region_name eq 'AL031658.11.1.162976');
+
+ok($seg2 && $seg2->[0] == 9);
+ok($seg2 && $seg2->[1] == 201);
+ok($seg2 && $seg2->[2]->start == 13588);
+ok($seg2 && $seg2->[2]->end == 13780);
+ok($seg2 && $seg2->[2]->seq_region_name eq 'AL359765.6.1.13780');
+
+debug('negative strand slice feature projection');
+foreach my $segment (@projection) {
+ ($start, $end, $slice) = @$segment;
+ debug("[$start-$end] -> " . $slice->seq_region_name
+ . ' ' . $slice->start . '-' . $slice->end . '('.$slice->strand().')');
+}
+
+#
+# Try project to same coord system
+#
+
+
+@projection = @{$feature->project('supercontig')};
+
+ok(@projection == 1);
+
+($seg1) = @projection;
+$slice = $feature->slice();
+ok($seg1 && $seg1->[0] == 1 );
+ok($seg1 && $seg1->[1] == $feature->length() );
+ok($seg1 && $seg1->[2]->start == $slice->end - $feature->end() + 1);
+ok($seg1 && $seg1->[2]->end == $slice->end - $feature->start() + 1);
+ok($seg1 && $seg1->[2]->strand == $slice->strand * $feature->strand());
+ok($seg1 && $seg1->[2]->seq_region_name eq 'NT_028392');
+
+
+debug('projection to same coord system');
+foreach my $segment (@projection) {
+ ($start, $end, $slice) = @$segment;
+ debug("[$start-$end] -> " . $slice->seq_region_name
+ . ' ' . $slice->start . '-' . $slice->end . '('.$slice->strand().')');
+}
+
+ok($feature->display_id eq '');
\ No newline at end of file
diff --git a/modules/t/featurePair.t b/modules/t/featurePair.t
new file mode 100644
index 0000000000..b692331013
--- /dev/null
+++ b/modules/t/featurePair.t
@@ -0,0 +1,97 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 23;
+}
+
+use TestUtils qw( debug test_getter_setter );
+
+use Bio::EnsEMBL::FeaturePair;
+use Bio::EnsEMBL::Slice;
+use Bio::EnsEMBL::Analysis;
+use Bio::EnsEMBL::CoordSystem;
+use MultiTestDB;
+
+our $verbose= 0; #turn on or off debug statements
+
+my $coord_system = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => 'chromosome',
+ -VERSION => 'NCBI34',
+ -DBID => 123,
+ -TOP_LEVEL => 1);
+
+my $analysis = Bio::EnsEMBL::Analysis->new(-LOGIC_NAME => 'test');
+
+my $slice = Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $coord_system,
+ -SEQ_REGION_NAME => 'X',
+ -START => 1_000_000,
+ -END => 2_000_000);
+
+#
+# Test new and getters
+#
+my $start = 10;
+my $end = 100;
+my $strand = -1;
+my $hstart = 1;
+my $hend = 90;
+my $hstrand = 1;
+my $hseqname = 'RF1231';
+my $percent_id = 90.8;
+my $p_value = '1.52';
+my $score = 50;
+my $species = 'Homo_sapiens';
+my $hspecies = 'Mus_musculus';
+
+my $fp = Bio::EnsEMBL::FeaturePair->new(-START => $start,
+ -END => $end,
+ -STRAND => $strand,
+ -ANALYSIS => $analysis,
+ -SLICE => $slice,
+ -HSTART => $hstart,
+ -HSTRAND => $hstrand,
+ -HEND => $hend,
+ -HSEQNAME => $hseqname,
+ -PERCENT_ID => $percent_id,
+ -P_VALUE => $p_value,
+ -SCORE => $score,
+ -SPECIES => $species,
+ -HSPECIES => $hspecies);
+
+
+ok($fp && $fp->isa('Bio::EnsEMBL::FeaturePair'));
+
+ok($fp->start == $start);
+ok($fp->end == $end);
+ok($fp->strand == $strand);
+ok($fp->analysis == $analysis);
+ok($fp->slice == $slice);
+
+ok($fp->hstart == $hstart);
+ok($fp->hend == $hend);
+ok($fp->hseqname eq $hseqname);
+ok($fp->percent_id == $percent_id);
+ok($fp->p_value == $p_value);
+ok($fp->score == $score);
+ok($fp->species eq $species);
+ok($fp->hspecies eq $hspecies);
+
+
+#
+# Test setters
+#
+ok(test_getter_setter($fp, 'hstart', 1002));
+ok(test_getter_setter($fp, 'hend', 2002));
+ok(test_getter_setter($fp, 'hseqname', 'test'));
+ok(test_getter_setter($fp, 'percent_id', 123.2));
+ok(test_getter_setter($fp, 'p_value', 23.0));
+ok(test_getter_setter($fp, 'score', 123));
+ok(test_getter_setter($fp, 'species', 'Rattus_norvegicus'));
+ok(test_getter_setter($fp, 'hspecies', 'Danio_rerio'));
+
+
+ok($fp->display_id eq $fp->hseqname());
diff --git a/modules/t/gene.t b/modules/t/gene.t
index ac46bbefd2..65386b798f 100644
--- a/modules/t/gene.t
+++ b/modules/t/gene.t
@@ -2,18 +2,21 @@ use lib 't';
use strict;
use warnings;
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test;
- plan tests => 39;
+ plan tests => 49;
}
use MultiTestDB;
use TestUtils qw ( debug test_getter_setter );
-
+use Bio::EnsEMBL::Exon;
+use Bio::EnsEMBL::FeaturePair;
+use Bio::EnsEMBL::Transcript;
+use Bio::EnsEMBL::Translation;
use Bio::EnsEMBL::Gene;
+use Bio::EnsEMBL::DnaDnaAlignFeature;
# switch on the debug prints
-
our $verbose = 0;
debug( "Startup test" );
@@ -42,12 +45,7 @@ $gene = $ga->fetch_by_stable_id( "ENSG00000171456" );
debug( "Gene->fetch_by_stable_id()" );
ok( $gene );
-my $e_slice = Bio::EnsEMBL::Slice->new
- ( -empty => 1,
- -adaptor => $db->get_SliceAdaptor()
- );
-$gene->transform( $e_slice );
debug( "Gene dbID: ". $gene->dbID());
ok( $gene->dbID() == 18267 );
@@ -84,7 +82,7 @@ ok( scalar @$links == 6 );
my $sa = $db->get_SliceAdaptor();
-my $slice = $sa->fetch_by_chr_start_end( "20", 30_249_935, 31_254_640 );
+my $slice = $sa->fetch_by_region( "chromosome", "20", 30_249_935, 31_254_640 );
debug( "Slice from SliceAdaptor" );
ok($slice);
@@ -114,7 +112,7 @@ $ex1->start( 13586 );
$ex1->end( 13735 );
$ex1->phase(0);
$ex1->end_phase( 0 );
-$ex1->contig( $slice );
+$ex1->slice( $slice );
$ex1->strand(1);
$ex1->analysis($analysis);
@@ -125,7 +123,7 @@ $fp->start(13586);
$fp->end (13705);
$fp->strand(1);
$fp->score(10);
-$fp->contig($slice);
+$fp->slice($slice);
$fp->hstart(100);
$fp->hend (219);
$fp->hstrand (1);
@@ -139,8 +137,7 @@ $fp->start(13707);
$fp->end (13735);
$fp->strand(1);
$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
+$fp->slice($slice);
$fp->hstart (220);
$fp->hend (248);
@@ -163,7 +160,7 @@ $ex2->start(201372);
$ex2->end(201571);
$ex2->phase(0);
$ex2->end_phase( -1 );
-$ex2->contig( $slice );
+$ex2->slice( $slice );
$ex2->strand(1);
$ex2->analysis($analysis);
@@ -174,7 +171,7 @@ $fp->start(201372);
$fp->end (201471);
$fp->strand(1);
$fp->score(10);
-$fp->contig($slice);
+$fp->slice( $slice );
$fp->hstart(100);
$fp->hend (199);
$fp->hstrand (1);
@@ -188,8 +185,8 @@ $fp->start(201472);
$fp->end (201571);
$fp->strand(1);
$fp->score(10);
-$fp->contig($slice);
-$fp->seqname(1);
+$fp->slice( $slice );
+
$fp->hstart (201);
$fp->hend (300);
@@ -210,7 +207,7 @@ $ex3->start(210600);
$ex3->end(210800);
$ex3->phase(-1);
$ex3->end_phase( -1 );
-$ex3->contig( $slice );
+$ex3->slice( $slice );
$ex3->strand(1);
$ex3->analysis($analysis);
@@ -249,9 +246,9 @@ my $translates = 1;
foreach my $tr( @{$gene->get_all_Transcripts()} ) {
if( $tr->translate()->seq() =~ /\*./ ) {
$translates = 0;
- debug( "Translate failed.".$tr->translate()->seq() );
+ debug( "Translate failed." );
}
-
+ debug( "Translation: ".$tr->translate()->seq() );
foreach my $exon ( @{$tr->get_all_Exons()} ) {
debug( " Exon start: ". $exon->start());
debug( " Exon end: ". $exon->end() );
@@ -265,14 +262,17 @@ ok( $translates );
ok( scalar(@{$gene->get_all_Exons()} ) == 3);
-$gene->transform();
-$multi->hide( "core", "gene", "transcript", "exon", "exon_transcript", "gene_description", "translation", "gene_stable_id", "transcript_stable_id", "exon_stable_id", "translation_stable_id", "supporting_feature", "dna_align_feature" );
+$gene = $gene->transform( "chromosome" );
-my $gene_ad = $db->get_GeneAdaptor();
-my $desc = 'test description';
+my $desc = 'test description for a gene';
+my $stable_id = 'ENSG00000171456';
$gene->description($desc);
-debug( "Storing the gene" );
+$gene->stable_id($stable_id);
+
+$multi->hide( "core", "meta_coord", "gene", "transcript", "exon", "exon_transcript", "gene_description", "translation", "gene_stable_id", "transcript_stable_id", "exon_stable_id", "translation_stable_id", "supporting_feature", "dna_align_feature" );
+my $gene_ad = $db->get_GeneAdaptor();
+debug( "Storing the gene" );
$gene_ad->store($gene);
ok(1);
@@ -280,11 +280,16 @@ ok(1);
my $genes = $slice->get_all_Genes();
-
ok(scalar( @$genes) == 1 );
my $gene_out = $genes->[0];
+#make sure the stable_id was stored
+ok($gene_out->stable_id eq $stable_id);
+
+#make sure the description was stored
+ok($gene_out->description eq $desc);
+
ok(scalar(@{$gene_out->get_all_Exons()}) == 3);
@@ -303,7 +308,6 @@ ok( $exons->[0]->start == 13586 );
ok( $exons->[1]->strand == 1 );
ok( $exons->[1]->phase == 0 );
-ok($gene_out->description eq $desc);
my $pep;
@@ -317,7 +321,7 @@ foreach my $trans( @{$gene_out->get_all_Transcripts()} ){
$translate = 1;
} else {
$translate = 0;
- }
+ }
}
ok($translate == 1);
@@ -328,13 +332,12 @@ my $e = $t->get_all_Exons()->[0];
my $se = $e->get_all_supporting_features();
debug( "Got ".scalar( @$se )." supporting features." );
-ok( scalar( @$se ) == 2 );
+ok( scalar( @$se ) == 1 );
-my $se_start = $se->[0]->start() < $se->[1]->start() ?
- $se->[0]->start() : $se->[1]->start();
+my $se_start = $se->[0]->start();
-my $se_end = $se->[0]->end() > $se->[1]->end() ?
- $se->[0]->end() : $se->[1]->end();
+
+my $se_end = $se->[0]->end();
debug( "Supporting start $se_start, end $se_end" );
@@ -357,7 +360,8 @@ debug( "checking external references" );
$multi->restore();
-$slice = $db->get_SliceAdaptor()->fetch_by_chr_start_end("20", 30_252_000, 31_252_001 );
+$slice = $db->get_SliceAdaptor()->fetch_by_region
+ ( "toplevel", "20", 30_252_000, 31_252_001 );
my $known = 0;
my $unknown = 0;
@@ -385,6 +389,9 @@ ok( $known==17 );
#}
+#save contents of gene table
+$multi->save('core', 'gene');
+
# tests for update method
# go get a fresh gene again
$gene = $ga->fetch_by_stable_id( "ENSG00000171456" );
@@ -403,6 +410,87 @@ $gene->display_xref( $dbEntryAdaptor->fetch_by_dbID( 614 ));
$gene->type('dummy');
$ga->update($gene);
-$newgene = $ga->fetch_by_stable_id( "ENSG00000171456" );
+$newgene = $ga->fetch_by_stable_id( "ENSG00000171456" );
ok ( $newgene->display_xref->dbID() == 614 );
ok ( $newgene->type eq 'dummy' );
+
+$multi->restore('core', 'gene');
+
+
+#
+# test GeneAdaptor::fetch_all_by_domain
+#
+my @genes = @{$ga->fetch_all_by_domain('IPR000010')};
+
+debug("Fetch by domain 'IPR000010'");
+
+ok(@genes == 2);
+debug("Got " . scalar(@genes) . " genes");
+ok(($genes[0]->stable_id() eq 'ENSG00000131044') ||
+ ($genes[1]->stable_id() eq 'ENSG00000131044'));
+ok(($genes[0]->stable_id() eq 'ENSG00000174873') ||
+ ($genes[1]->stable_id() eq 'ENSG00000174873'));
+
+
+#
+# test GeneAdaptor::fetch_all_by_external_name
+#
+
+#Q15691
+($gene) = @{$ga->fetch_all_by_external_name('MAE1_HUMAN')};
+debug($gene->stable_id);
+ok($gene->stable_id() eq 'ENSG00000101367');
+
+#
+# test GeneAdaptor::get_Interpro_by_geneid
+#
+debug("Test get_Interpro_by_geneid");
+my @interpro = @{$ga->get_Interpro_by_geneid('ENSG00000174873')};
+ok(@interpro == 1);
+debug($interpro[0]);
+
+
+ok($gene->display_id eq $gene->stable_id);
+
+#
+# test Gene: get_all_alt_alleles
+#
+
+$gene = $ga->fetch_by_dbID( 18256 );
+my $alt_genes = $gene->get_all_alt_alleles();
+
+ok( scalar( @$alt_genes ) == 3 );
+
+# expect the following alleles
+my %gene_ids = ( 18257 => 1, 18258 => 1, 18259 => 1);
+my $ok = 1;
+for my $gene ( @$alt_genes ) {
+ $ok = $ok && $gene_ids{$gene->dbID()};
+}
+ok( $ok );
+
+#
+# test storing a new allele group
+#
+
+$multi->hide( 'core', 'alt_allele' );
+
+my @alt_genes;
+push( @alt_genes, $ga->fetch_by_dbID(18270) );
+push( @alt_genes, $ga->fetch_by_dbID(18271) );
+push( @alt_genes, $ga->fetch_by_dbID(18272) );
+$ga->store_alt_alleles( \@alt_genes );
+
+$gene = $ga->fetch_by_dbID( 18270 );
+$alt_genes = $gene->get_all_alt_alleles();
+%gene_ids = ( 18271=>1, 18272=>1 );
+
+$ok = 1;
+for my $gene ( @$alt_genes ) {
+ $ok = $ok && $gene_ids{$gene->dbID()};
+}
+ok( $ok );
+
+
+$multi->restore('core');
+
diff --git a/modules/t/geneview.t b/modules/t/geneview.t
index 2b2f914dcb..e55dc9d0ab 100644
--- a/modules/t/geneview.t
+++ b/modules/t/geneview.t
@@ -29,7 +29,7 @@ my $gene = $db->get_GeneAdaptor->fetch_by_transcript_stable_id( "ENST00000217347
my $geneid = $gene->stable_id;
ok( $geneid );
-$gene = $db->get_GeneAdaptor->fetch_by_Peptide_id( "ENSP00000278995" );
+$gene = $db->get_GeneAdaptor->fetch_by_translation_stable_id( "ENSP00000278995" );
$geneid = $gene->stable_id;
$gene = $db->get_GeneAdaptor()->fetch_by_stable_id( "ENSG00000101321" );
diff --git a/modules/t/karyotypeBand.t b/modules/t/karyotypeBand.t
index 697e426d30..8c786c1b85 100644
--- a/modules/t/karyotypeBand.t
+++ b/modules/t/karyotypeBand.t
@@ -3,34 +3,56 @@ use warnings;
use lib 't';
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test;
- plan tests => 7;
+ plan tests => 10;
}
use TestUtils qw(debug test_getter_setter);
use Bio::EnsEMBL::KaryotypeBand;
-
+use Bio::EnsEMBL::Slice;
+use Bio::EnsEMBL::CoordSystem;
#
-#1 TEST - KaryotypeBand compiles
+# Test constructor
#
-ok(1);
-#
-# 2 test constructor
-#
-my $kb = Bio::EnsEMBL::KaryotypeBand->new;
-ok($kb->isa('Bio::EnsEMBL::KaryotypeBand'));
+my $coord_system = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => 'chromosome',
+ -VERSION => 'NCBI34',
+ -DBID => 123,
+ -TOP_LEVEL => 1);
+
+
+my $slice = Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $coord_system,
+ -SEQ_REGION_NAME => 'X',
+ -START => 1,
+ -END => 2e6);
+
+my $start = 1;
+my $end = 1e6;
+my $name = 'q.11';
+my $stain = 'gpos50';
+my $kb = Bio::EnsEMBL::KaryotypeBand->new(-START => $start,
+ -END => $end,
+ -STAIN => $stain,
+ -NAME => $name,
+ -SLICE => $slice);
+
+ok($kb->start() == $start);
+ok($kb->end() == $end);
+ok($kb->stain() eq $stain);
+ok($kb->name() eq $name);
+ok($kb->slice == $slice);
+ok($kb->display_id eq $name);
#
-# 3-7 test getter/setters
+# test getter/setters
#
ok(test_getter_setter($kb, 'name', 'p.31'));
-ok(test_getter_setter($kb, 'chr_name', 'X'));
ok(test_getter_setter($kb, 'start', 12_200_000));
ok(test_getter_setter($kb, 'end', 13_000_000));
-ok(test_getter_setter($kb, 'stain', 'gpos50'));
+ok(test_getter_setter($kb, 'stain', 'gpos60'));
diff --git a/modules/t/karyotypeBandAdaptor.t b/modules/t/karyotypeBandAdaptor.t
new file mode 100644
index 0000000000..8f6abda114
--- /dev/null
+++ b/modules/t/karyotypeBandAdaptor.t
@@ -0,0 +1,87 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 18;
+}
+
+
+use MultiTestDB;
+use TestUtils qw(debug test_getter_setter);
+use Bio::EnsEMBL::KaryotypeBand;
+
+our $verbose= 0;
+
+my $multi_db = MultiTestDB->new;
+my $db = $multi_db->get_DBAdaptor('core');
+
+
+my $kba = $db->get_KaryotypeBandAdaptor();
+
+ok(ref($kba) && $kba->isa('Bio::EnsEMBL::DBSQL::KaryotypeBandAdaptor'));
+
+
+my $slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ '20',
+ 30_000_000,
+ 32_000_000);
+
+
+
+
+my $bands = $kba->fetch_all_by_Slice($slice);
+
+print_features($bands);
+
+ok(@$bands == 1);
+
+my ($band) = @$bands;
+
+ok($band->name() eq 'q11.21');
+ok($band->start() == 200001);
+ok($band->end() == 2600001);
+ok($band->strand() == 0);
+
+
+
+($band) = @{$kba->fetch_all_by_chr_name('20')};
+
+print_features([$band]);
+
+ok($band->name() eq 'q11.21');
+ok($band->slice->seq_region_name eq '20');
+ok($band->start() == 30_200_000);
+ok($band->end() == 32_600_000);
+
+
+($band) = @{$kba->fetch_all_by_chr_band('20', 'q11')};
+
+print_features([$band]);
+
+ok($band->name() eq 'q11.21');
+ok($band->slice->seq_region_name eq '20');
+ok($band->start() == 30_200_000);
+ok($band->end() == 32_600_000);
+
+$band = $kba->fetch_by_dbID(1);
+print_features([$band]);
+ok($band->name() eq 'q11.21');
+ok($band->slice->seq_region_name eq '20');
+ok($band->start() == 30_200_000);
+ok($band->end() == 32_600_000);
+
+sub print_features {
+ my $features = shift;
+
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ debug('['.$f->dbID.']'.$f->start.'-'.$f->end.'('.$f->strand.')'.
+ ' '.$f->name(). ' '.$f->stain());
+ } else {
+ debug('UNDEF');
+ }
+ }
+}
diff --git a/modules/t/mapLocation.t b/modules/t/mapLocation.t
index 910ecd154e..275c780416 100644
--- a/modules/t/mapLocation.t
+++ b/modules/t/mapLocation.t
@@ -7,7 +7,6 @@ BEGIN { $| = 1;
}
use Bio::EnsEMBL::Map::MapLocation;
-use Bio::EnsEMBL::Chromosome;
use MultiTestDB;
use TestUtils qw(debug test_getter_setter);
@@ -25,12 +24,12 @@ my $db = $multi->get_DBAdaptor( 'core' );
#test constructor
my $mapname = 'genethon';
my $name = 'DS1234';
-my $chr = $db->get_ChromosomeAdaptor->fetch_by_chr_name('X');
+my $chr_name = 'X';
my $pos = '12.5';
my $lod = 0.23;
my $mloc =
- Bio::EnsEMBL::Map::MapLocation->new($name, $mapname, $chr, $pos, $lod);
+ Bio::EnsEMBL::Map::MapLocation->new($name, $mapname, $chr_name, $pos, $lod);
ok($mloc && ref $mloc && $mloc->isa('Bio::EnsEMBL::Map::MapLocation'));
@@ -49,9 +48,9 @@ ok(&test_getter_setter($mloc, 'map_name', 'marshfield'));
# 4-5 #
#######
-#test chromosome
-ok($chr == $mloc->chromosome);
-ok(&test_getter_setter($mloc, 'chromosome', undef));
+#test chromosome_name
+ok($chr_name eq $mloc->chromosome_name);
+ok(&test_getter_setter($mloc, 'chromosome_name', undef));
#######
# 6-7 #
@@ -59,7 +58,7 @@ ok(&test_getter_setter($mloc, 'chromosome', undef));
#test name
ok($name eq $mloc->name);
-ok(&test_getter_setter($mloc, 'name', 'Z123213'));
+ok(&test_getter_setter($mloc, 'name', 'Z123213'));
#######
diff --git a/modules/t/mapper.t b/modules/t/mapper.t
index 45ef489b3c..63ee548c90 100644
--- a/modules/t/mapper.t
+++ b/modules/t/mapper.t
@@ -25,7 +25,7 @@
## We start with some black magic to print on failure.
BEGIN { $| = 1;
use Test;
- plan tests => 5; # 5 tests total
+ plan tests => 10; # 5 tests total
use vars qw($loaded); }
END { print "not ok 1\n" unless $loaded; }
@@ -64,6 +64,93 @@ test_transform ($mapper,
+#
+# check if the mapper can do merging
+#
+
+$mapper = Bio::EnsEMBL::Mapper->new( "asm1", "asm2" );
+
+$mapper->add_map_coordinates( "1", 1, 10, 1, "1", 101, 110 );
+$mapper->add_map_coordinates( "1", 21, 30, 1, "1", 121, 130 );
+$mapper->add_map_coordinates( "1", 11, 20, 1, "1", 111, 120 );
+
+test_transform( $mapper,
+ [ "1", 5, 25, 1, "asm1" ],
+ [ "1", 105, 125, 1 ] );
+
+
+
+#
+# Slightly differnt merge case
+#
+$mapper = Bio::EnsEMBL::Mapper->new( "asm1", "asm2" );
+
+$mapper->add_map_coordinates( "1", 1, 10, 1, "1", 101, 110 );
+$mapper->add_map_coordinates( "1", 21, 30, 1, "1", 121, 130 );
+$mapper->add_map_coordinates( "1", 12, 20, 1, "1", 112, 120 );
+
+test_transform( $mapper,
+ [ "1", 5, 25, 1, "asm1" ],
+ [ "1", 105, 110, 1 ],
+ [ "1", 11, 11, 0 ],
+ [ "1", 112, 125, 1 ] );
+
+
+
+#
+# dont merge on wrong orientation
+#
+
+$mapper = Bio::EnsEMBL::Mapper->new( "asm1", "asm2" );
+
+$mapper->add_map_coordinates( "1", 1, 10, 1, "1", 101, 110 );
+$mapper->add_map_coordinates( "1", 21, 30, 1, "1", 121, 130 );
+$mapper->add_map_coordinates( "1", 11, 20, -1, "1", 111, 120 );
+
+test_transform( $mapper,
+ [ "1", 5, 25, 1, "asm1" ],
+ [ "1", 105, 110, 1 ],
+ [ "1", 111, 120, -1 ],
+ [ "1", 121, 125, 1 ] );
+
+#
+# can reverse strands merge?
+#
+
+$mapper = Bio::EnsEMBL::Mapper->new( "asm1", "asm2" );
+
+$mapper->add_map_coordinates( "1", 1, 10, -1, "1", 121, 130 );
+$mapper->add_map_coordinates( "1", 21, 30, -1, "1", 101, 110 );
+$mapper->add_map_coordinates( "1", 11, 20, -1, "1", 111, 120 );
+
+test_transform( $mapper,
+ [ "1", 5, 25, 1, "asm1" ],
+ [ "1", 106, 126, -1 ] );
+
+
+#
+# normal merge, not three
+#
+
+$mapper = Bio::EnsEMBL::Mapper->new( "asm1", "asm2" );
+
+$mapper = Bio::EnsEMBL::Mapper->new( "asm1", "asm2" );
+
+$mapper->add_map_coordinates( "1", 1, 10, 1, "1", 101, 110 );
+$mapper->add_map_coordinates( "1", 11, 20, 1, "1", 111, 120 );
+$mapper->add_map_coordinates( "1", 22, 30, 1, "1", 132, 140 );
+$mapper->add_map_coordinates( "1", 51, 70, 1, "1", 161, 180 );
+$mapper->add_map_coordinates( "1", 31, 35, 1, "1", 141, 145 );
+
+test_transform( $mapper,
+ [ "1", 5, 45, 1, "asm1" ],
+ [ "1", 105, 120, 1 ],
+ [ "1", 21, 21, 0 ],
+ [ "1", 132, 145, 1 ],
+ [ "1", 36, 45, 0 ]
+ );
+
+
# the following subroutine tests that a given source co-ordinate range
# transforms into a given set of destination co-ordinates
#
@@ -74,6 +161,7 @@ test_transform ($mapper,
# for @dest array, $strand=0 indicates gap.
# for @dest array, $id=$srcid for gaps.
+
sub test_transform {
my ($mapper, $src, @dest) = @_;
if (@$src != 5) { warn "Bad source coords: (@$src)\n"; printnotok(); return }
diff --git a/modules/t/marker.t b/modules/t/marker.t
index 0525b3c099..56a7df40f2 100644
--- a/modules/t/marker.t
+++ b/modules/t/marker.t
@@ -33,7 +33,7 @@ my $priority = 80;
#test the constructor
my $marker = Bio::EnsEMBL::Map::Marker->new
- ($dbID, $marker_adaptor, $left_primer, $right_primer, $min_primer_dist,
+ ($dbID, $marker_adaptor, $left_primer, $right_primer, $min_primer_dist,
$max_primer_dist, $priority);
ok($marker && ref $marker && $marker->isa('Bio::EnsEMBL::Map::Marker'));
@@ -154,7 +154,7 @@ ok(scalar @{$marker->get_all_MapLocations} == 2 );
######
#test get_MapLocation
-ok($marker->get_MapLocation('genethon') &&
+ok($marker->get_MapLocation('genethon') &&
$marker->get_MapLocation('marshfield'));
#########
diff --git a/modules/t/markerFeature.t b/modules/t/markerFeature.t
index cb49be4193..a3c69cffba 100644
--- a/modules/t/markerFeature.t
+++ b/modules/t/markerFeature.t
@@ -3,12 +3,11 @@ use strict;
BEGIN { $| = 1;
use Test ;
- plan tests => 11
+ plan tests => 12
}
use MultiTestDB;
use Bio::EnsEMBL::Map::MarkerFeature;
-use Bio::EnsEMBL::RawContig;
use Bio::EnsEMBL::Analysis;
use TestUtils qw(debug test_getter_setter);
@@ -27,7 +26,9 @@ my $adaptor = $db->get_MarkerFeatureAdaptor;
my $dbID = 111;
my $start = 100;
my $end = 10;
-my $contig = Bio::EnsEMBL::RawContig->new;
+my $slice =
+ $db->get_SliceAdaptor->fetch_by_region('contig','AL359765.6.1.13780');
+
my $analysis = Bio::EnsEMBL::Analysis->new;
my $marker_id = 1;
my $mapweight = 1;
@@ -39,7 +40,7 @@ my $mapweight = 1;
#test construction
my $mf = Bio::EnsEMBL::Map::MarkerFeature->new
- ($dbID, $adaptor, $start, $end, $contig, $analysis, $marker_id,
+ ($dbID, $adaptor, $start, $end, $slice, $analysis, $marker_id,
$mapweight);
ok($mf && ref $mf && $mf->isa('Bio::EnsEMBL::Map::MarkerFeature'));
@@ -76,7 +77,7 @@ ok($marker->dbID == $marker_id);
#test contig, start, end, strand (inherited)
-ok($contig == $mf->contig);
+ok($slice == $mf->slice);
ok($start == $mf->start);
ok($end == $mf->end);
ok($mf->strand == 0);
@@ -86,3 +87,6 @@ ok($mf->strand == 0);
#######
ok($mapweight == $mf->map_weight);
+
+
+ok($mf->display_id eq $mf->marker->display_MarkerSynonym());
\ No newline at end of file
diff --git a/modules/t/markerFeatureAdaptor.t b/modules/t/markerFeatureAdaptor.t
index fcc3138132..73a7334f20 100644
--- a/modules/t/markerFeatureAdaptor.t
+++ b/modules/t/markerFeatureAdaptor.t
@@ -34,7 +34,7 @@ my $mf = $mfa->fetch_by_dbID(1);
ok($mf && ref $mf && $mf->isa('Bio::EnsEMBL::Map::MarkerFeature'));
-ok($mf->contig->dbID == 339816 &&
+ok($mf->slice->seq_region_name eq 'AL353092.6.1.25010' &&
$mf->analysis->dbID == 10 &&
$mf->start == 5769 &&
$mf->end == 5959 &&
@@ -49,9 +49,10 @@ ok($mf->contig->dbID == 339816 &&
# test fetch all by slice
-my $slice = $db->get_SliceAdaptor->fetch_by_chr_start_end('20',
- '30249935',
- '31254640');
+my $slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ '20',
+ '30249935',
+ '31254640');
my $feats = $mfa->fetch_all_by_Slice($slice);
@@ -92,12 +93,12 @@ ok(scalar(@$feats) == 1 && $feats->[0]->start==12671 &&
$multi->hide('core', 'marker_feature');
$marker = $db->get_MarkerAdaptor->fetch_by_dbID(80);
-my $contig = $db->get_RawContigAdaptor->fetch_by_dbID(317101);
+$slice = $db->get_SliceAdaptor->fetch_by_seq_region_id(317101);
my $analysis = $db->get_AnalysisAdaptor->fetch_by_dbID(10);
my $marker_feature = Bio::EnsEMBL::Map::MarkerFeature->new;
-$marker_feature->contig($contig);
+$marker_feature->slice($slice);
$marker_feature->start(123);
$marker_feature->end(200);
$marker_feature->marker($marker);
@@ -105,7 +106,7 @@ $marker_feature->analysis($analysis);
$mfa->store($marker_feature);
-ok($marker_feature->dbID &&
+ok($marker_feature->dbID &&
$marker_feature->adaptor == $mfa);
diff --git a/modules/t/metaContainer.t b/modules/t/metaContainer.t
index 52379ff5e7..71b7326279 100644
--- a/modules/t/metaContainer.t
+++ b/modules/t/metaContainer.t
@@ -3,9 +3,9 @@ use warnings;
use lib 't';
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test;
- plan tests => 9;
+ plan tests => 8;
}
use TestUtils qw( debug );
@@ -28,7 +28,7 @@ ok($mc);
#
-# 2 - list_value_by_key
+# list_value_by_key
#
my ($asm_default) = @{$mc->list_value_by_key('assembly.default')};
@@ -36,7 +36,7 @@ ok($asm_default eq 'NCBI_30');
#
-# 3-4 store key value
+# store key value
#
$mc->store_key_value('testkey', 'testvalue1');
@@ -46,9 +46,14 @@ my $listref = $mc->list_value_by_key('testkey');
ok($listref->[0] eq 'testvalue1');
ok($listref->[1] eq 'testvalue2');
+$mc->delete_key('testkey');
+
+$listref = $mc->list_value_by_key('testkey');
+ok(@$listref == 0);
+
#
-# 5-6 - get_Species
+# get_Species
#
my $species = $mc->get_Species();
@@ -62,18 +67,5 @@ ok($bin eq 'Homo sapiens');
my $taxid = $mc->get_taxonomy_id();
ok($taxid == 9606);
-
-#
-# 8 - get_default_assembly
-#
-$asm_default = $mc->get_default_assembly();
-ok($asm_default eq 'NCBI_30');
-
-#
-# 9 - get_max_assembly_contig
-#
-my $maxac = $mc->get_max_assembly_contig();
-ok($maxac == 1_000_000);
-
$mdb->restore('core', 'meta');
diff --git a/modules/t/miscFeature.t b/modules/t/miscFeature.t
new file mode 100644
index 0000000000..5d29c0a4d9
--- /dev/null
+++ b/modules/t/miscFeature.t
@@ -0,0 +1,87 @@
+use lib 't';
+use strict;
+
+BEGIN { $| = 1;
+ use Test ;
+ plan tests => 11
+}
+
+use MultiTestDB;
+use Bio::EnsEMBL::MiscFeature;
+use Bio::EnsEMBL::MiscSet;
+use TestUtils qw(debug test_getter_setter);
+
+our $verbose = 0; #set to 1 to turn on debug printouts
+
+
+#test constructor
+my $mf = Bio::EnsEMBL::MiscFeature->new(-START => 10,
+ -END => 100);
+
+ok($mf->start() == 10 && $mf->end() == 100);
+
+
+
+#
+# Test add_set, get_set, get_set_codes
+#
+my $ms1 = Bio::EnsEMBL::MiscSet->new(3, undef,
+ '1mbcloneset',
+ '1mb Clone Set',
+ 'This is a 1MB cloneset',
+ 1e7);
+
+my $ms2 = Bio::EnsEMBL::MiscSet->new(4, undef,
+ 'tilepath',
+ 'Tiling Path',
+ 'NCBI33 Tiling Path',
+ 1e6);
+
+
+
+$mf->add_set($ms1);
+$mf->add_set($ms2);
+
+
+ok($mf->get_set($ms1->code) == $ms1);
+ok($mf->get_set($ms2->code) == $ms2);
+
+my @codes = $mf->get_set_codes;
+
+ok(@codes == 2);
+
+@codes = grep {($_ eq $ms1->code()) || ($_ eq $ms2->code())} @codes;
+
+ok(@codes == 2);
+
+#
+# Test add_attribute, get_attribute_types, get_attribute
+#
+
+my $name1 = 'test name';
+my $name2 = 'AL4231124.1';
+
+$mf->add_attribute('name', $name1);
+
+ok($mf->display_id eq $name1);
+
+$mf->add_attribute('name', $name2);
+$mf->add_attribute('version', '4');
+
+my @types = $mf->get_attribute_types();
+ok(@types == 2);
+
+@types = grep {$_ eq 'name' || $_ eq 'version'} @types;
+
+ok(@types == 2);
+
+my @attribs = $mf->get_attribute('name');
+
+ok(@attribs == 2);
+
+@attribs = grep {$_ eq $name1 || $_ eq $name2} @attribs;
+
+ok(@attribs == 2);
+
+@attribs = $mf->get_attribute('version');
+ok(@attribs == 1 && $attribs[0] eq '4');
diff --git a/modules/t/miscFeatureAdaptor.t b/modules/t/miscFeatureAdaptor.t
new file mode 100644
index 0000000000..9868abaa0a
--- /dev/null
+++ b/modules/t/miscFeatureAdaptor.t
@@ -0,0 +1,106 @@
+use strict;
+
+use lib 't';
+use TestUtils qw(test_getter_setter debug);
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 11;
+}
+
+use MultiTestDB;
+
+our $verbose = 0;
+
+my $multi = MultiTestDB->new;
+
+# get a core DBAdaptor
+my $dba = $multi->get_DBAdaptor("core");
+
+
+#
+# Test get_MiscFeatureAdaptor works
+#
+my $mfa = $dba->get_MiscFeatureAdaptor();
+
+ok($mfa && ref($mfa) && $mfa->isa('Bio::EnsEMBL::DBSQL::MiscFeatureAdaptor'));
+
+
+#
+# Test fetching by slice
+#
+
+
+my $chr_slice = $dba->get_SliceAdaptor->fetch_by_region('chromosome', '20');
+my $features = $mfa->fetch_all_by_Slice($chr_slice);
+debug('--- chr 20 misc_features ---');
+debug("Got " . scalar(@$features));
+ok(@$features == 7);
+print_features($features);
+
+
+$features = $mfa->fetch_all_by_Slice_and_set_code($chr_slice,'ntctgs');
+debug('--- chr 20 ntcontigs set---');
+debug("Got " . scalar(@$features));
+ok(@$features == 3);
+print_features($features);
+
+$features = $mfa->fetch_all_by_Slice_and_set_code($chr_slice,'cloneset');
+debug('--- chr 20 cloneset set---');
+debug("Got " . scalar(@$features));
+ok(@$features == 0);
+print_features($features);
+
+my $feature = $mfa->fetch_by_dbID(1);
+debug('--- fetch_by_dbID ---');
+ok($feature->dbID() == 1);
+ok($feature->start() == 61140848);
+ok($feature->end() == 62842997);
+ok($feature->strand() == 1);
+print_features([$feature]);
+
+
+#
+# Test fetching by attribute
+#
+
+debug("--- fetch by attribute (superctg) ---");
+$features = $mfa->fetch_all_by_attribute_type_value('superctg');
+
+ok(@$features == 7);
+print_features($features);
+
+debug("--- fetch by attribute (superctg, NT_035608) ---");
+$features = $mfa->fetch_all_by_attribute_type_value('superctg','NT_035608');
+
+ok(@$features == 1);
+print_features($features);
+
+debug("--- fetch by attribute (embl_acc) ---");
+$features = $mfa->fetch_all_by_attribute_type_value('embl_acc');
+ok(@$features == 1);
+print_features($features);
+
+
+
+sub print_features {
+ my $features = shift;
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ my @attrib_types = $f->get_attribute_types;
+ my @attrib_vals = map {"$_ => " . join(',',$f->get_attribute($_))} @attrib_types;
+
+ my @set_codes = $f->get_set_codes;
+ my @set_names = map {"$_ => " . $f->get_set($_)->name()} @set_codes;
+
+ my $attrib_string = join(":", @attrib_vals);
+ my $set_string = join(":", @set_names);
+
+ my $seqname = $f->slice->seq_region_name();
+ debug($seqname . ' ' . $f->start().'-'.$f->end().'('.$f->strand().
+ ') ['. $f->dbID.'] '." ($attrib_string) ($set_string)");
+ } else {
+ debug('UNDEF');
+ }
+ }
+}
diff --git a/modules/t/miscSet.t b/modules/t/miscSet.t
new file mode 100644
index 0000000000..0ad0d0b107
--- /dev/null
+++ b/modules/t/miscSet.t
@@ -0,0 +1,45 @@
+use lib 't';
+use strict;
+
+BEGIN { $| = 1;
+ use Test ;
+ plan tests => 10
+}
+
+use Bio::EnsEMBL::MiscSet;
+use TestUtils qw(debug test_getter_setter);
+
+our $verbose = 0; #set to 1 to turn on debug printouts
+
+
+
+my $misc_set_id = 3;
+my $code = '1mbcloneset';
+my $name = '1mb Clone Set';
+my $desc = 'This is a 1MB cloneset';
+my $max_len = 1e7;
+
+
+#
+# Test constructor
+#
+my $ms = Bio::EnsEMBL::MiscSet->new($misc_set_id,
+ undef,
+ $code,
+ $name,
+ $desc,
+ $max_len);
+
+ok($ms->dbID == $misc_set_id);
+ok($ms->code eq $code);
+ok($ms->description eq $desc);
+ok($ms->longest_feature eq $max_len);
+ok($ms->name eq $name);
+
+ok(test_getter_setter($ms, 'dbID', 12));
+ok(test_getter_setter($ms, 'code', 'testcode'));
+ok(test_getter_setter($ms, 'description', 'new description'));
+ok(test_getter_setter($ms, 'longest_feature', 1e8));
+ok(test_getter_setter($ms, 'name', $name));
+
+
diff --git a/modules/t/miscSetAdaptor.t b/modules/t/miscSetAdaptor.t
new file mode 100644
index 0000000000..1511990d20
--- /dev/null
+++ b/modules/t/miscSetAdaptor.t
@@ -0,0 +1,57 @@
+use lib 't';
+use strict;
+
+BEGIN { $| = 1;
+ use Test ;
+ plan tests => 12
+}
+
+use MultiTestDB;
+use Bio::EnsEMBL::MiscSet;
+use TestUtils qw(debug test_getter_setter);
+
+our $verbose = 0; #set to 1 to turn on debug printouts
+
+
+my $multi_db = MultiTestDB->new;
+my $db = $multi_db->get_DBAdaptor('core');
+
+
+#
+# Test constructor
+#
+
+my $msa = $db->get_MiscSetAdaptor();
+
+
+ok($msa && ref($msa) && $msa->isa('Bio::EnsEMBL::DBSQL::MiscSetAdaptor'));
+
+
+#
+# Test fetch_all
+#
+
+ok(@{$msa->fetch_all()} == 4);
+
+#
+# Test fetch_by_dbID
+#
+
+my $ms = $msa->fetch_by_dbID(1);
+
+ok($ms->dbID(1));
+ok($ms->code() eq 'ntctgs');
+ok($ms->name() eq 'NT contigs');
+ok($ms->description eq '');
+ok($ms->longest_feature == 7e7);
+
+#
+# Test fetch_by_code
+#
+
+$ms = $msa->fetch_by_code('cloneset');
+ok($ms->dbID() == 4);
+ok($ms->code() eq 'cloneset');
+ok($ms->name() eq '1Mb cloneset');
+ok($ms->description eq '');
+ok($ms->longest_feature == 6e6);
diff --git a/modules/t/predictionTranscript.t b/modules/t/predictionTranscript.t
index 9bf3f9a3bf..a779edc352 100644
--- a/modules/t/predictionTranscript.t
+++ b/modules/t/predictionTranscript.t
@@ -5,13 +5,12 @@ use lib 't';
BEGIN { $| = 1;
use Test;
- plan tests => 34;
+ plan tests => 39;
}
use MultiTestDB;
use TestUtils qw(debug test_getter_setter);
use Bio::EnsEMBL::PredictionTranscript;
-use Bio::EnsEMBL::Exon;
our $verbose = 0;
@@ -26,208 +25,283 @@ my $db = $multi->get_DBAdaptor( "core" );
my $sa = $db->get_SliceAdaptor();
-my $slice = $sa->fetch_by_chr_start_end("20", 30_252_000, 31_252_001 );
+my $slice = $sa->fetch_by_region("chromosome", "20", 30_252_000, 31_252_001 );
my $p_transs = $slice->get_all_PredictionTranscripts();
#
# 2 Verify prediciton transcripts can be obtained
#
+debug( "Retrieved ".scalar( @$p_transs )." PredictionTranscripts." );
ok( scalar( @$p_transs ) );
-if($verbose) {
- foreach my $pt (@$p_transs) {
- print $pt->stable_id , "\n";
- }
+foreach my $pt (@$p_transs) {
+ debug( "Stable_id: ".$pt->stable_id()." Start: ".$pt->start." End: ".$pt->end() , "\n" );
}
-my ($pt) = @$p_transs;
+my $pt = $p_transs->[0];
my $exons = $pt->get_all_Exons;
#
# 3 test get all exons
#
+debug( "First PT had ".scalar( @$exons ). " exons." );
ok(scalar @$exons);
#
# 4 test new
#
-my $new_pt = new Bio::EnsEMBL::PredictionTranscript(@$exons);
+my $new_pt = new Bio::EnsEMBL::PredictionTranscript( -exons => $exons);
ok(scalar @{$new_pt->get_all_Exons});
#
# 5 test stable_id
#
-ok($pt->stable_id =~ /(\w+\.\d+\.\d+\.\d+)\.(\d+)\.(\d+)/);
-#my ($ctg, $ctg_start, $ctg_end) = ($1, $2, $3);
+debug( "stable_id: ".$pt->stable_id() );
+ok($pt->stable_id =~ /.*/ );
#
-# 6 test coding start
-#
-ok(&TestUtils::test_getter_setter($pt, 'coding_region_start', 6));
-
-#
-# 7 test coding end
-#
-ok(&TestUtils::test_getter_setter($pt, 'coding_region_end', 7));
-
-
-#
-# 8 test start
+# 6 test start
#
ok(&TestUtils::test_getter_setter($pt, 'start', 8));
#
-# 9 test end
+# 7 test end
#
ok(&TestUtils::test_getter_setter($pt, 'end', 9));
#
-# 10 test analysis
+# 8 test analysis
#
my $analysis = $db->get_AnalysisAdaptor->fetch_by_logic_name('Vertrna');
ok(&TestUtils::test_getter_setter($pt, 'analysis', $analysis));
#
-# 11 test dbID
+# 9 test dbID
#
ok(&TestUtils::test_getter_setter($pt, 'dbID', 11));
#
-# 12 test adaptor
+# 10 test adaptor
#
my $pta = $db->get_PredictionTranscriptAdaptor;
ok(&TestUtils::test_getter_setter($pt, 'adaptor', $pta));
#
-# 13-17 test add Exon
+# 11-14 test add Exon
#
+$pt = new Bio::EnsEMBL::PredictionTranscript();
+
my $exon = new Bio::EnsEMBL::Exon;
-$exon->start($pt->start - 20);
-$exon->end($pt->end + 20);
+$exon->start(40);
+$exon->end(50);
+$exon->slice( $slice );
+$exon->strand( 1 );
+$pt->add_Exon($exon);
-my @old_exons = @{$pt->get_all_Exons};
-my $count = scalar(@old_exons);
+$exon = new Bio::EnsEMBL::Exon;
+$exon->start(20);
+$exon->end(30);
+$exon->slice( $slice );
+$exon->strand( 1 );
+$pt->add_Exon($exon);
+$exon = new Bio::EnsEMBL::Exon;
+$exon->start( 1 );
+$exon->end(10);
+$exon->slice( $slice );
+$exon->strand( 1 );
$pt->add_Exon($exon);
+
+
#check that transcript start + end updated
-ok($pt->start == $exon->start);
-ok($pt->end == $exon->end);
-$exons = $pt->get_all_Exons( 1 );
-#check that there is one more exon
-ok($count + 1 == scalar(@$exons));
-#check that the last exon is the exon added
-ok($exons->[$#$exons] eq $exon);
+ok( $pt->end() == 50 );
+ok( $pt->start() == 1 );
+
+my $all_exons = $pt->get_all_Exons();
+ok( $all_exons->[0]->start() == 1 );
+ok( $all_exons->[2]->end() == 50 );
+
+#
+# 15-18 -1 strand checks for add_Exon
+#
+
+$pt = new Bio::EnsEMBL::PredictionTranscript();
+
+$exon = new Bio::EnsEMBL::Exon;
+$exon->start(40);
+$exon->end(50);
+$exon->slice( $slice );
+$exon->strand( -1 );
+$pt->add_Exon($exon);
+
+$exon = new Bio::EnsEMBL::Exon;
+$exon->start( 1 );
+$exon->end(10);
+$exon->slice( $slice );
+$exon->strand( -1 );
+$pt->add_Exon($exon);
+
+$exon = new Bio::EnsEMBL::Exon;
+$exon->start(20);
+$exon->end(30);
+$exon->slice( $slice );
+$exon->strand( -1 );
+$pt->add_Exon($exon);
+
+ok( $pt->end() == 50 );
+ok( $pt->start() == 1 );
+
+$all_exons = $pt->get_all_Exons();
+ok( $all_exons->[0]->start() == 40 );
+ok( $all_exons->[2]->end() == 10 );
+
+
+
-$pt->add_Exon($exon, 3);
-#check that third exon is exon added
-ok($exons->[2] eq $exon);
#
-# 18-22 test flush exons
+# 19-23 test flush exons
#
+debug( "Flush exons effect test" );
$pt->flush_Exons;
ok(scalar @{$pt->get_all_Exons} == 0);
+debug( "pt->start ".($pt->start()||"undef") );
ok(!defined $pt->start);
+debug( "pt->end ".($pt->end()||"undef") );
ok(!defined $pt->end);
-ok(!defined $pt->coding_start);
-ok(!defined $pt->coding_end);
-
-#restore old exons
-my $pos = 0;
-while(@old_exons) {
- $pos++;
- my $e = shift @old_exons;
- $pt->add_Exon($e, $pos);
-}
+debug( "pt->coding_start ".($pt->coding_region_start()||"undef") );
+ok(!defined $pt->coding_region_start);
+debug( "pt->coding_end ".($pt->coding_region_end()||"undef") );
+ok(!defined $pt->coding_region_end);
-#
-# 23 test get_all_translateable_Exons
-#
-ok(scalar @{$pt->get_all_translateable_Exons} == scalar @{$pt->get_all_Exons});
#
-# 24 test sort executes
+# 24 test get_all_translateable_Exons
#
-ok($pt->sort || 1);
+$pt = $p_transs->[0];
-#
-# 25 test get_exon_count
-#
-ok($pt->get_exon_count == scalar @{$pt->get_all_Exons});
+ok(scalar @{$pt->get_all_translateable_Exons} == scalar @{$pt->get_all_Exons});
-#
-# 26 test set_exon_count
-#
-$count = $pt->get_exon_count;
-$pt->set_exon_count(26);
-ok(scalar @{$pt->get_all_Exons( 1 )} == 26); # test internal exon array expansion
-$pt->set_exon_count($count);
#
-# 27 test length
+# 25 test length
#
my $len = 0;
foreach my $ex (@{$pt->get_all_Exons}) {
- if( defined $ex ) { $len += $ex->length };
+ $len += $ex->length();
}
ok($len == $pt->length);
#
-# 28 test translate
+# 26 test translate
#
-ok(length $pt->translate->seq);
+my $translated = $pt->translate->seq();
+debug( "Translated sequence: $translated" );
+ok( $translated );
#
-# 29 test get cdna
+# 27 test spliced_seq()
#
-my $cstart = 1;
-my $cend = $pt->length;
-ok(length $pt->get_cdna($cstart, $cend));
+my $spliced_seq = $pt->spliced_seq();
+debug( "Spliced seq: ".$spliced_seq );
+ok( $spliced_seq );
#
-# 30 test pep2genomic
+# 28 test pep2genomic
#
my $pend = $pt->length / 3;
my $pstart = 2;
my $defined_exons_count = 0;
foreach my $e (@{$pt->get_all_Exons}) {
- if(defined $e) {
$defined_exons_count++;
- }
}
# should return genomic coords for each exon since covers entire peptide
ok($defined_exons_count == $pt->pep2genomic($pstart, $pend));
#
-# 31 test cdna2genomic
+# 29 test cdna2genomic
#
-ok($defined_exons_count == $pt->cdna2genomic($cstart, $cend));
+
+ok($defined_exons_count == $pt->cdna2genomic( 1, $pt->length()));
#
-# 32 test type
+# 30 test type
#
ok(&TestUtils::test_getter_setter($pt, 'type', 'test'));
#
-# 33 test fetch_by_stable_id
+# 31 test fetch_by_stable_id
#
-my $stable_id = 'AL031658.11.1.162976.122801.143660';
+my $stable_id = 'GENSCAN00000011401';
$pt = $pta->fetch_by_stable_id($stable_id);
ok($pt->stable_id eq $stable_id);
-# 34 list_dbIDs
+# 32 list_dbIDs
my $ids = $pta->list_dbIDs();
ok (@{$ids});
+ok($pt->display_id eq $pt->stable_id);
+
+
+#
+# test PredictionTranscriptAdaptor::store
+#
+
+my $analysis_adaptor = $db->get_AnalysisAdaptor();
+
+$analysis = $analysis_adaptor->fetch_by_logic_name('Genscan');
+
+$multi->hide('core', 'prediction_transcript', 'prediction_exon');
+
+my @exons;
+push @exons, Bio::EnsEMBL::PredictionExon->new
+ (-START => 100,
+ -END => 200,
+ -STRAND => 1,
+ -P_VALUE => 0.98,
+ -SCORE => 50,
+ -SLICE => $slice,
+ -PHASE => 0);
+
+push @exons, Bio::EnsEMBL::PredictionExon->new
+ (-START => 300,
+ -END => 400,
+ -STRAND => 1,
+ -P_VALUE => 0.99,
+ -SCORE => 75,
+ -SLICE => $slice,
+ -PHASE => $exons[0]->length % 3);
+
+$pt = Bio::EnsEMBL::PredictionTranscript->new
+ (-EXONS => \@exons,
+ -SLICE => $slice,
+ -ANALYSIS => $analysis);
+
+$pta->store($pt);
+
+$pt = $pta->fetch_by_dbID($pt->dbID);
+
+ok($pt && $pt->dbID);
+
+@exons = @{$pt->get_all_Exons};
+ok(@exons == 2);
+
+ok($exons[0]->start == $slice->start + 100 - 1);
+ok($exons[1]->start == $slice->start + 300 - 1);
+
+ok($exons[0]->score == 50);
+ok($exons[1]->score == 75);
+
+$multi->restore();
diff --git a/modules/t/proteinAlignFeatureAdaptor.t b/modules/t/proteinAlignFeatureAdaptor.t
index aad36fbea4..558c256e92 100644
--- a/modules/t/proteinAlignFeatureAdaptor.t
+++ b/modules/t/proteinAlignFeatureAdaptor.t
@@ -2,13 +2,15 @@ use lib 't';
BEGIN { $| = 1;
use Test;
- plan tests => 2;
+ plan tests => 22;
}
use MultiTestDB;
use TestUtils qw(test_getter_setter debug);
+use Bio::EnsEMBL::DnaPepAlignFeature;
+
our $verbose = 0;
my $multi = MultiTestDB->new();
@@ -22,3 +24,139 @@ debug("ProteinAlignFeatureAdaptor->list_dbIDs");
my $ids = $pafa->list_dbIDs();
ok (@{$ids});
+
+
+
+#
+# Test retrieval via Slice
+#
+
+my $feats;
+
+my $chr_slice = $db->get_SliceAdaptor->fetch_by_region('chromosome','20',
+ 30_500_000, 30_700_000);
+
+$feats = $pafa->fetch_all_by_Slice($chr_slice);
+debug('---fetching by chromosomal slice---');
+debug("Got " . scalar(@$feats) . " features back");
+ok(@$feats == 2429);
+print_features($feats);
+
+
+my $ctg_slice;
+$ctg_slice = $db->get_SliceAdaptor->fetch_by_region('contig',
+ 'AL031658.11.1.162976',
+ 1,
+ 50_000);
+$feats = $pafa->fetch_all_by_Slice($ctg_slice);
+debug('--- contig AL031658.11.1.162976 (1-50000) features ---');
+debug("Got " . scalar(@$feats));
+ok(@$feats == 357);
+ print_features($feats);
+
+
+#
+# Test fetch_by_dbID
+#
+my $feat = $pafa->fetch_by_dbID(5339568);
+debug('--- fetching by dbID ---');
+ok($feat);
+print_features([$feat]);
+
+
+$feat = $feat->transform('supercontig');
+debug('--- transforming to supercontig coords ---');
+ok($feat);
+print_features([$feat]);
+
+
+#
+# Test fetch_by_Slice_and_pid
+#
+$feats = $pafa->fetch_all_by_Slice_and_pid($chr_slice, '90');
+debug('--- fetching by chr Slice and pid (90) ---');
+debug("Got " . scalar(@$feats));
+ok(@$feats == 64);
+print_features($feats);
+
+#
+# Test store
+#
+
+$multi->save('core', 'protein_align_feature');
+
+my $analysis = $feat->analysis;
+my $slice =
+ $db->get_SliceAdaptor->fetch_by_region('contig','AL031658.11.1.162976');
+my $start = 100;
+my $end = 200;
+my $strand = 1;
+my $hstart = 10;
+my $hend = 110;
+my $hstrand = -1;
+my $hseqname = 'test';
+my $score = 80;
+my $percent_id = 90;
+my $evalue = 23.2;
+my $cigar_string = '100M';
+
+$feat = Bio::EnsEMBL::DnaPepAlignFeature->new
+ (-START => $start,
+ -END => $end,
+ -STRAND => $strand,
+ -SLICE => $slice,
+ -HSTART => $hstart,
+ -HEND => $hend,
+ -HSTRAND => 1,
+ -HSEQNAME => $hseqname,
+ -CIGAR_STRING => '100M',
+ -PERCENT_ID => $percent_id,
+ -SCORE => $score,
+ -P_VALUE => $evalue,
+ -ANALYSIS => $analysis);
+
+ok(!$feat->is_stored($db));
+
+$pafa->store($feat);
+
+ok($feat->is_stored($db));
+
+my $dbID = $feat->dbID();
+
+$feat = $pafa->fetch_by_dbID($dbID);
+
+ok($feat->dbID == $dbID);
+ok($feat->start == $start);
+ok($feat->end == $end);
+ok($feat->strand == $strand);
+ok($feat->slice->name eq $slice->name);
+ok($feat->hstart == $hstart);
+ok($feat->hend == $hend);
+ok($feat->hseqname eq $hseqname);
+ok($feat->cigar_string eq $cigar_string);
+ok($feat->percent_id == $percent_id);
+ok($feat->score == $score);
+ok($feat->p_value == $evalue);
+ok($feat->analysis->logic_name eq $analysis->logic_name);
+
+$multi->restore('core', 'protein_align_feature');
+
+
+
+
+sub print_features {
+ return if(!$verbose);
+ my $features = shift;
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ my $seqname = $f->slice->seq_region_name();
+ my $analysis = $f->analysis->logic_name();
+ debug($seqname . ' ' . $f->start().'-'.$f->end().'('.$f->strand().
+ ') ['. $f->dbID.'] '. $f->cigar_string . ' ' .
+ $f->hstart .'-'.$f->hend.' ('.$f->hstrand.')'.$f->score() .
+ " ($analysis) " . $f->percent_id);
+ } else {
+ debug('UNDEF');
+ }
+ }
+}
diff --git a/modules/t/proteinFeature.t b/modules/t/proteinFeature.t
new file mode 100644
index 0000000000..dd333ddcb9
--- /dev/null
+++ b/modules/t/proteinFeature.t
@@ -0,0 +1,86 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 17;
+}
+
+use TestUtils qw( debug test_getter_setter );
+
+use Bio::EnsEMBL::ProteinFeature;
+use Bio::EnsEMBL::Analysis;
+
+our $verbose = 0; #turn on or off debug statements
+
+
+#
+# Test new and getters
+#
+my $start = 10;
+my $end = 100;
+my $hstart = 1;
+my $hend = 90;
+my $hstrand = 1;
+my $hseqname = 'RF1231';
+my $percent_id = 90.8;
+my $p_value = '1.52';
+my $score = 50;
+my $species = 'Homo_sapiens';
+my $hspecies = 'Mus_musculus';
+
+my $idesc = 'interpro description';
+my $interpro_ac = 'interpro accession';
+
+my $analysis = Bio::EnsEMBL::Analysis->new(-LOGIC_NAME => 'test');
+
+
+my $f = Bio::EnsEMBL::ProteinFeature->new
+ (-START => $start,
+ -END => $end,
+ -ANALYSIS => $analysis,
+ -HSTART => $hstart,
+ -HEND => $hend,
+ -HSEQNAME => $hseqname,
+ -PERCENT_ID => $percent_id,
+ -P_VALUE => $p_value,
+ -SCORE => $score,
+ -SPECIES => $species,
+ -HSPECIES => $hspecies,
+ -IDESC => $idesc,
+ -INTERPRO_AC => $interpro_ac);
+
+
+
+ok($f && $f->isa('Bio::EnsEMBL::ProteinFeature'));
+
+ok($f->start == $start);
+ok($f->end == $end);
+ok($f->analysis == $analysis);
+
+ok($f->hstart == $hstart);
+ok($f->hend == $hend);
+ok($f->hseqname eq $hseqname);
+ok($f->percent_id == $percent_id);
+ok($f->p_value == $p_value);
+ok($f->score == $score);
+ok($f->species eq $species);
+ok($f->hspecies eq $hspecies);
+
+ok($f->idesc eq $idesc);
+ok($f->interpro_ac eq $interpro_ac);
+
+# check that the strand is 0
+ok($f->strand == 0);
+
+#
+# Test setters
+#
+ok(test_getter_setter($f, 'idesc', 'interpro desc1'));
+ok(test_getter_setter($f, 'interpro_ac', 'interpro ac1'));
+
+
+
+
diff --git a/modules/t/proteinFeatureAdaptor.t b/modules/t/proteinFeatureAdaptor.t
new file mode 100644
index 0000000000..795170d48c
--- /dev/null
+++ b/modules/t/proteinFeatureAdaptor.t
@@ -0,0 +1,43 @@
+use strict;
+
+use lib 't';
+use TestUtils qw(test_getter_setter debug);
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 2;
+}
+
+use MultiTestDB;
+
+our $verbose = 0;
+
+my $multi = MultiTestDB->new;
+
+# get a core DBAdaptor
+my $dba = $multi->get_DBAdaptor("core");
+
+
+#
+# Test get_ProteinFeatureAdaptor works
+#
+my $pfa = $dba->get_ProteinFeatureAdaptor();
+
+ok($pfa && ref($pfa) && $pfa->isa('Bio::EnsEMBL::DBSQL::ProteinFeatureAdaptor'));
+
+
+my $pfs = $pfa->fetch_all_by_translation_id(21724);
+
+print_features($pfs);
+
+ok(@$pfs == 15);
+
+sub print_features {
+ my $features = shift;
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ debug($f->start.'-'.$f->end. ' -> '.$f->hseqname. ':'.
+ $f->hstart. '-'.$f->hend);
+ }
+ }
+}
diff --git a/modules/t/qtl.t b/modules/t/qtl.t
index b6765e4514..ac9b1b555a 100644
--- a/modules/t/qtl.t
+++ b/modules/t/qtl.t
@@ -4,7 +4,7 @@ use warnings;
BEGIN { $| = 1;
use Test ;
- plan tests => 7;
+ plan tests => 6;
}
use MultiTestDB;
@@ -25,7 +25,7 @@ my $sa = $db->get_SliceAdaptor();
-my $slice = $sa->fetch_by_chr_name( "20" );
+my $slice = $sa->fetch_by_region( 'chromosome', "20" );
my $qtl_features = $slice->get_all_QtlFeatures();
@@ -56,8 +56,4 @@ my $synonyms = $qtl->get_synonyms;
ok($synonyms->{'rat genome database'} eq 'rqtl2');
-my @result = $qf->transform();
-
-debug( join("\n", @result ));
-ok( scalar( @result ) == 9 );
diff --git a/modules/t/rangeRegistry.t b/modules/t/rangeRegistry.t
new file mode 100644
index 0000000000..657560cbb7
--- /dev/null
+++ b/modules/t/rangeRegistry.t
@@ -0,0 +1,115 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 21;
+}
+
+use TestUtils qw( debug );
+
+use Bio::EnsEMBL::Mapper::RangeRegistry;
+
+our $verbose= 0;
+
+my $rr = Bio::EnsEMBL::Mapper::RangeRegistry->new();
+
+my $id = 'ID1';
+
+#expect [100,400] back
+my $range = $rr->check_and_register($id, 200,300, 100,400);
+ok(@$range==1 && $range->[0]->[0] == 100 && $range->[0]->[1] == 400);
+print_ranges($range);
+
+#expect undef back
+$range = $rr->check_and_register($id, 150,190, 100,200);
+ok(!defined($range));
+print_ranges($range);
+
+#expect [401,500] back
+$range = $rr->check_and_register($id, 200, 500);
+ok(@$range==1 && $range->[0]->[0] == 401 && $range->[0]->[1] == 500);
+print_ranges($range);
+
+#expect undef back
+$range = $rr->check_and_register($id, 300, 500);
+ok(!defined($range));
+print_ranges($range);
+
+#expect 700-900 back
+$range = $rr->check_and_register($id, 700, 900);
+ok(@$range==1 && $range->[0]->[0] == 700 && $range->[0]->[1] == 900);
+print_ranges($range);
+
+# expect 1000-1200 back
+$range = $rr->check_and_register($id, 1050, 1150, 1000, 1200);
+ok(@$range==1 && $range->[0]->[0] == 1000 && $range->[0]->[1] == 1200);
+print_ranges($range);
+
+#expect 50-99, 501-699, 901-950 back
+$range = $rr->check_and_register($id, 50, 200, 50, 950);
+ok(@$range==3 && $range->[0]->[0] == 50 && $range->[0]->[1] == 99);
+ok(@$range==3 && $range->[1]->[0] == 501 && $range->[1]->[1] == 699);
+ok(@$range==3 && $range->[2]->[0] == 901 && $range->[2]->[1] == 950);
+print_ranges($range);
+
+#make sure that the interal list has been merged into 2 ranges
+#we have to do this to make sure that it is efficient
+my $internal_list = $rr->{'registry'}->{$id};
+ok(@$internal_list == 2);
+
+#check that creating adjacent regions merges the list correctly
+$range = $rr->check_and_register($id, 40,45,30,49);
+ok(@$internal_list == 2);
+ok(@$range==1 && $range->[0]->[0] == 30 && $range->[0]->[1] == 49);
+print_ranges($range);
+
+$range = $rr->check_and_register($id, 951, 999);
+ok(@$internal_list == 1);
+ok($range && $range->[0]->[0] == 951 && $range->[0]->[1] == 999);
+print_ranges($range);
+
+
+# Check that a single range can be added to the beginning
+$range = $rr->check_and_register($id, 1, 10, 1,20);
+ok(@$internal_list == 2);
+ok(@$range==1 && $range->[0]->[0] == 1 && $range->[0]->[1] == 20);
+print_ranges($range);
+
+#check range that spans entire existing ranges
+$range = $rr->check_and_register($id, 1, 1200);
+ok(@$internal_list == 1);
+ok(@$range==1 && $range->[0]->[0] == 21 && $range->[0]->[1] == 29);
+print_ranges($range);
+
+#check adding identical range to existing internal range
+$range = $rr->check_and_register($id, 1, 1200);
+ok(!defined($range));
+print_ranges($range);
+
+
+#check requesting small area of size 1
+$range = $rr->check_and_register($id,10,10, 1, 1e6);
+ok(!defined($range));
+print_ranges($range);
+
+#check that adding a range to a different id works
+$range = $rr->check_and_register("ID2", 100,500, 1, 600);
+ok($range && @$range==1 && $range->[0]->[0] == 1 && $range->[0]->[1] == 600);
+print_ranges($range);
+
+
+
+sub print_ranges {
+ my $rangelist = shift;
+ if(!defined($rangelist)) {
+ debug("UNDEF");
+ return;
+ }
+
+ foreach my $range (@$rangelist) {
+ debug('['.$range->[0].'-'.$range->[1].']');
+ }
+}
diff --git a/modules/t/rawContig.t b/modules/t/rawContig.t
index 85fc774f9f..65f37c9d28 100755
--- a/modules/t/rawContig.t
+++ b/modules/t/rawContig.t
@@ -8,6 +8,17 @@ BEGIN { $| = 1;
use MultiTestDB;
use TestUtils qw(debug);
+use Bio::EnsEMBL::Utils::Exception qw(verbose);
+
+######################################################################
+#
+# Clone is a deprecated class but needed for backwards
+# compatibility. These tests ensure that it actually works,
+# but verbosity is turned off to avoid all of the deprecated warnings
+#
+#######################################################################
+
+verbose(-1);
our $verbose = 0; #set to 1 for debug printing
@@ -61,7 +72,7 @@ my $repseq = $contig->get_repeatmasked_seq( );
debug( "Repeatmasked: ".substr( $repseq->seq(), 0, 50 ) );
debug( " isa ".ref( $repseq ));
-ok( $repseq->isa( "Bio::PrimarySeq" ));
+ok( $repseq->isa( "Bio::EnsEMBL::RepeatMaskedSlice" ));
my $sFeatures = $contig->get_all_SimilarityFeatures( "swall" );
@@ -103,3 +114,7 @@ ok( $a == 6395 &&
$n == 0 &&
$gc_content == 48.28 &&
$a + $t + $c + $g + $n == $contig->length());
+
+
+
+verbose(0);
diff --git a/modules/t/rawContigAdaptor.t b/modules/t/rawContigAdaptor.t
index 93abebdc7a..ab84ac0369 100644
--- a/modules/t/rawContigAdaptor.t
+++ b/modules/t/rawContigAdaptor.t
@@ -5,7 +5,7 @@ use warnings;
BEGIN { $| = 1;
use Test;
- plan tests => 41;
+ plan tests => 26;
}
use MultiTestDB;
@@ -14,6 +14,19 @@ use Bio::EnsEMBL::RawContig;
use TestUtils qw(test_getter_setter);
use Bio::Seq;
+use Bio::EnsEMBL::Utils::Exception qw(verbose);
+
+######################################################################
+#
+# RawContigAdaptor is a deprecated class but needed for backwards
+# compatibility. These tests ensure that it actually works,
+# but verbosity is turned off to avoid all of the deprecated warnings
+#
+#######################################################################
+
+verbose(-1);
+
+
#
#1 slice adaptor compiles
#
@@ -69,7 +82,7 @@ my $contig3 = $contigs_from_clone->[0];
ok ($contig3->dbID == 368744);
ok ($contig3->name eq "AL359765.6.1.13780");
ok ($contig3->length == 13780);
-ok ($contig3->clone->dbID == 26799);
+ok ($contig3->clone->name eq "AL359765.6");
#
@@ -83,7 +96,7 @@ ok($unpop_contig->name eq 'AL133343.23.1.63609');
ok($unpop_contig->length == 63609);
ok($unpop_contig->dbID == 469270);
ok($unpop_contig->embl_offset == 1);
-ok($unpop_contig->clone->dbID == 34957);
+ok($unpop_contig->clone->name eq "AL133343.23");
#
@@ -114,109 +127,113 @@ ok($first_contig->name eq 'AL359765.6.1.13780');
ok($first_contig->length == 13780);
ok($first_contig->dbID == 368744);
ok($first_contig->embl_offset == 1);
-ok($first_contig->clone->dbID == 26799);
+ok($first_contig->clone->name eq "AL359765.6");
-#
-# 26-32 check out the store
-#
+##
+## 26-32 check out the store
+##
-# save the original state of the contig table
-$multi->save("core","contig","dna");
+## save the original state of the contig table
+#$multi->save("core","contig","dna");
-my $dname = 'dummy_contig';
-my $dummy_contig = Bio::EnsEMBL::RawContig->new;
-$dummy_contig->name($dname);
-$dummy_contig->embl_offset(7);
-$dummy_contig->length(24);
+#my $dname = 'dummy_contig';
+#my $dummy_contig = Bio::EnsEMBL::RawContig->new;
+#$dummy_contig->name($dname);
+#$dummy_contig->embl_offset(7);
+#$dummy_contig->length(24);
-my $seq = Bio::Seq->new(-seq => 'ATGCAGCTAGCATCGATGACATCG',
- -id => 'dummy_contig',
- -accession => 'dummy_contig');
-ok($seq);
+#my $seq = Bio::Seq->new(-seq => 'ATGCAGCTAGCATCGATGACATCG',
+# -id => 'dummy_contig',
+# -accession => 'dummy_contig');
+#ok($seq);
-$dummy_contig->seq($seq);
+#$dummy_contig->seq($seq);
-$raw_adaptor->store($dummy_contig, $clone);
-ok($raw_adaptor);
+#$raw_adaptor->store($dummy_contig, $clone);
+#ok($raw_adaptor);
-#
-# manual check to see whether the contig has gone in
-#
-my $sth = $db->prepare("select * from contig");
-$sth->execute;
-#print STDERR "Num contigs " . scalar($sth->rows) . "\n";
-ok(scalar($sth->rows) == 13);
+##
+## manual check to see whether the contig has gone in
+##
+#my $sth = $db->prepare("select * from contig");
+#$sth->execute;
+##print STDERR "Num contigs " . scalar($sth->rows) . "\n";
+#ok(scalar($sth->rows) == 13);
-#
-# and just to check, retrieve the stored items
-#
+##
+## and just to check, retrieve the stored items
+##
-my $stored_contig = $raw_adaptor->fetch_by_name($dname);
-ok($stored_contig->name eq $dname);
-ok($stored_contig->embl_offset == 7);
-ok($stored_contig->length == 24);
-ok($stored_contig->clone->isa('Bio::EnsEMBL::Clone'));
+#my $stored_contig = $raw_adaptor->fetch_by_name($dname);
+#ok($stored_contig->name eq $dname);
+#ok($stored_contig->embl_offset == 7);
+#ok($stored_contig->length == 24);
+#ok($stored_contig->clone->isa('Bio::EnsEMBL::Clone'));
-# restore the contig table
-$multi->restore("core","contig","dna");
+## restore the contig table
+#$multi->restore("core","contig","dna");
-#
-# 33-40 remove
-#
+##
+## 33-40 remove
+##
-# save the original state of the contig table
-$multi->save("core","contig","dna","repeat_feature","simple_feature",
- "dna_align_feature","protein_align_feature","prediction_transcript");
+## save the original state of the contig table
+#$multi->save("core","contig","dna","repeat_feature","simple_feature",
+# "dna_align_feature","protein_align_feature","prediction_transcript");
-# remove the contig AL031658.11.1.162976 from the db
-$raw_adaptor->remove($contig);
-ok($raw_adaptor);
+## remove the contig AL031658.11.1.162976 from the db
+#$raw_adaptor->remove($contig);
+#ok($raw_adaptor);
-# manual check to see whether the contig and associated
-#features have gone in
-#
+## manual check to see whether the contig and associated
+##features have gone in
+##
+
+#$sth = $db->prepare("select * from contig");
+#$sth->execute;
+#ok(scalar($sth->rows) == 11);
+
+#$sth = $db->prepare("select * from dna");
+#$sth->execute;
+#ok(scalar($sth->rows) == 11);
+
+## contig should have 419 records
+#$sth = $db->prepare("select * from repeat_feature");
+#$sth->execute;
+#ok(scalar($sth->rows) == 1937);
-$sth = $db->prepare("select * from contig");
-$sth->execute;
-ok(scalar($sth->rows) == 11);
+## contig should have 20 records
+#$sth = $db->prepare("select * from simple_feature");
+#$sth->execute;
+#ok(scalar($sth->rows) == 116);
-$sth = $db->prepare("select * from dna");
-$sth->execute;
-ok(scalar($sth->rows) == 11);
+## contig should have 11641 records
+#$sth = $db->prepare("select * from dna_align_feature");
+#$sth->execute;
+#ok(scalar($sth->rows) == 15525);
-# contig should have 419 records
-$sth = $db->prepare("select * from repeat_feature");
-$sth->execute;
-ok(scalar($sth->rows) == 1937);
+## contig should have 2507 records
+#$sth = $db->prepare("select * from protein_align_feature");
+#$sth->execute;
+#ok(scalar($sth->rows) == 4727);
-# contig should have 20 records
-$sth = $db->prepare("select * from simple_feature");
-$sth->execute;
-ok(scalar($sth->rows) == 116);
+## contig should have 30 records
+#$sth = $db->prepare("select * from prediction_transcript");
+#$sth->execute;
+#ok(scalar($sth->rows) == 161);
-# contig should have 11641 records
-$sth = $db->prepare("select * from dna_align_feature");
-$sth->execute;
-ok(scalar($sth->rows) == 15525);
+## restore the original state of the contig table
+#$multi->restore("core","contig","dna","repeat_feature","simple_feature",
+# "dna_align_feature","protein_align_feature","prediction_transcript");
-# contig should have 2507 records
-$sth = $db->prepare("select * from protein_align_feature");
-$sth->execute;
-ok(scalar($sth->rows) == 4727);
-# contig should have 30 records
-$sth = $db->prepare("select * from prediction_transcript");
-$sth->execute;
-ok(scalar($sth->rows) == 161);
-# restore the original state of the contig table
-$multi->restore("core","contig","dna","repeat_feature","simple_feature",
- "dna_align_feature","protein_align_feature","prediction_transcript");
+verbose(0);
\ No newline at end of file
diff --git a/modules/t/repeatConsensus.t b/modules/t/repeatConsensus.t
index 5480904a32..9b843aca7f 100644
--- a/modules/t/repeatConsensus.t
+++ b/modules/t/repeatConsensus.t
@@ -1,45 +1,45 @@
+use strict;
use lib 't';
use TestUtils qw(test_getter_setter);
-BEGIN { $| = 1;
+use Bio::EnsEMBL::RepeatConsensus;
+
+
+BEGIN { $| = 1;
use Test;
- plan tests => 13;
+ plan tests => 12;
}
-my $loaded = 0;
-END {print "not ok 1\n" unless $loaded;}
-
my $verbose = 0;
-use MultiTestDB;
-
-my $multi = MultiTestDB->new();
-
-$loaded = 1;
-
-ok(1);
-
-my $db = $multi->get_DBAdaptor( 'core' );
-
-$repeat_c_ad = $db->get_RepeatConsensusAdaptor();
-
-ok ($repeat_c_ad);
-
-my $repeat_consensus = Bio::EnsEMBL::RepeatConsensus->new();
-
-ok ($repeat_consensus);
-
-ok (test_getter_setter($repeat_consensus,'length',10));
-ok (test_getter_setter($repeat_consensus,'repeat_class','dummy'));
-ok (test_getter_setter($repeat_consensus,'name','dummy'));
-ok (test_getter_setter($repeat_consensus,'repeat_consensus','ATGCATGCAT'));
-ok (test_getter_setter($repeat_consensus,'dbID',42));
-
-ok ($repeat_consensus->desc eq 'class=dummy');
-ok ($repeat_consensus->moltype eq 'dna');
-ok ($repeat_consensus->alphabet eq 'dna');
-ok ($repeat_consensus->seq eq 'ATGCATGCAT');
-
-$repeat_c_ad->store($repeat_consensus);
-
-ok(1);
+my $consensus = 'actg';
+my $name = 'actg(n)';
+my $length = 4;
+my $class = 'Simple_repeat';
+my $dbID = 123;
+
+#
+# Test constructor
+#
+my $repeat_consensus = Bio::EnsEMBL::RepeatConsensus->new
+ (-REPEAT_CONSENSUS => $consensus,
+ -NAME => $name,
+ -LENGTH => $length,
+ -REPEAT_CLASS => $class,
+ -DBID => 123);
+
+ok ($repeat_consensus && ref($repeat_consensus) &&
+ $repeat_consensus->isa('Bio::EnsEMBL::RepeatConsensus'));
+
+ok($repeat_consensus->length() == $length);
+ok($repeat_consensus->repeat_consensus() eq $consensus);
+ok($repeat_consensus->seq() eq $consensus);
+ok($repeat_consensus->name() eq $name);
+ok($repeat_consensus->dbID() == $dbID);
+ok($repeat_consensus->repeat_class() eq $class);
+
+ok(test_getter_setter($repeat_consensus,'length',10));
+ok(test_getter_setter($repeat_consensus,'repeat_class','dummy'));
+ok(test_getter_setter($repeat_consensus,'name','dummy'));
+ok(test_getter_setter($repeat_consensus,'repeat_consensus','ATGCATGCAT'));
+ok(test_getter_setter($repeat_consensus,'dbID',42));
diff --git a/modules/t/repeatConsensusAdaptor.t b/modules/t/repeatConsensusAdaptor.t
new file mode 100644
index 0000000000..5ebbc5760b
--- /dev/null
+++ b/modules/t/repeatConsensusAdaptor.t
@@ -0,0 +1,87 @@
+use strict;
+use lib 't';
+use TestUtils qw(test_getter_setter);
+
+use MultiTestDB;
+
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 22;
+}
+
+my $multi_db = MultiTestDB->new;
+my $db = $multi_db->get_DBAdaptor('core');
+
+my $verbose = 0;
+
+# Test Creation
+
+my $rca = $db->get_RepeatConsensusAdaptor();
+
+ok(ref($rca) && $rca->isa('Bio::EnsEMBL::DBSQL::RepeatConsensusAdaptor'));
+
+#
+# Test fetch_by_dbID
+#
+
+my $rc = $rca->fetch_by_dbID(9);
+ok($rc->name() eq 'MIR3');
+ok($rc->dbID == 9);
+ok($rc->repeat_consensus eq '');
+ok($rc->length() == 0);
+ok($rc->repeat_class eq 'Type I Transposons/SINE');
+
+#
+# Test fetch_by_name
+#
+$rc = $rca->fetch_by_name('MIR');
+ok($rc->name() eq 'MIR');
+ok($rc->dbID() == 1);
+ok($rc->repeat_consensus eq '');
+ok($rc->length() == 0);
+ok($rc->repeat_class eq 'Type I Transposons/SINE');
+
+#
+# Test fetch_by_name_class
+#
+
+$rc = $rca->fetch_by_name_class('MER65A', 'LTRs');
+ok($rc->name() eq 'MER65A');
+ok($rc->dbID() == 283);
+ok($rc->repeat_class eq 'LTRs');
+ok($rc->repeat_consensus eq '');
+ok($rc->length() == 0);
+
+#
+# Test fetch_all_by_class_seq
+#
+ok(@{$rca->fetch_all_by_class_seq('LTRs', '')} == 38);
+
+#
+# Test store
+#
+
+$multi_db->save('core', 'repeat_consensus');
+
+$rc = Bio::EnsEMBL::RepeatConsensus->new
+ (-REPEAT_CONSENSUS => 'ACTG',
+ -NAME => 'ACTG(n)',
+ -LENGTH => 4,
+ -REPEAT_CLASS => 'Simple_repeat');
+
+
+$rca->store($rc);
+
+ok($rc->dbID && $rc->adaptor());
+
+$rc = $rca->fetch_by_dbID($rc->dbID);
+
+ok($rc->repeat_consensus eq 'ACTG');
+ok($rc->repeat_class eq 'Simple_repeat');
+ok($rc->length() == 4);
+ok($rc->name eq 'ACTG(n)');
+
+
+$multi_db->restore('core', 'repeat_consensus');
+
diff --git a/modules/t/repeatFeature.t b/modules/t/repeatFeature.t
index 8b5ddcb3dd..fdb31736da 100644
--- a/modules/t/repeatFeature.t
+++ b/modules/t/repeatFeature.t
@@ -1,93 +1,98 @@
+use strict;
+
+use Bio::EnsEMBL::RepeatFeature;
+use Bio::EnsEMBL::Slice;
+use Bio::EnsEMBL::Analysis;
+use Bio::EnsEMBL::RepeatConsensus;
+use Bio::EnsEMBL::CoordSystem;
+use Bio::EnsEMBL::RepeatFeature;
+
use lib 't';
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test;
- plan tests => 12;
+ plan tests => 16;
}
-use MultiTestDB;
use TestUtils qw(test_getter_setter debug);
our $verbose = 0;
-my $multi = MultiTestDB->new();
-
-ok(1);
-
-my $db = $multi->get_DBAdaptor( 'core' );
-
-$cadp = $db->get_RawContigAdaptor();
-
-$contig = $cadp->fetch_by_dbID(319456);
-
-my $analysis = $db->get_AnalysisAdaptor->fetch_by_logic_name("RepeatMask");
-
-debug( "ANALYSIS ".$analysis );
-
-ok($analysis);
-ok($contig);
-
-$repeat_f_ad = $db->get_RepeatFeatureAdaptor();
-$repeat_c_ad = $db->get_RepeatConsensusAdaptor();
-
-
-debug( "Analysis dbID ".$analysis->dbID );
-
-my $repeat_consensus = Bio::EnsEMBL::RepeatConsensus->new();
-
-$repeat_consensus->length(10);
-$repeat_consensus->repeat_class('dummy');
-$repeat_consensus->name('dummy');
-$repeat_consensus->repeat_consensus('ATGCATGCAT');
-
-ok($repeat_consensus);
-
-$repeat_c_ad->store($repeat_consensus);
-
-ok(1);
-
-my $repeat_feature = Bio::EnsEMBL::RepeatFeature->new();
+my $coord_system = Bio::EnsEMBL::CoordSystem->new
+ (-NAME => 'chromosome',
+ -VERSION => 'NCBI34',
+ -DBID => 123,
+ -TOP_LEVEL => 1);
-$repeat_feature->start(26);
-$repeat_feature->end(65);
-$repeat_feature->strand(1);
-$repeat_feature->hstart(6);
-$repeat_feature->hend(45);
-$repeat_feature->score(100);
-$repeat_feature->analysis($analysis);
-$repeat_feature->repeat_consensus($repeat_consensus);
-$repeat_feature->contig( $contig );
+my $analysis = Bio::EnsEMBL::Analysis->new(-LOGIC_NAME => 'test');
-ok($repeat_feature);
-$multi->hide( "core", "repeat_feature" );
+my $slice = Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $coord_system,
+ -SEQ_REGION_NAME => 'X',
+ -START => 1_000_000,
+ -END => 2_000_000);
-$repeat_f_ad->store( $repeat_feature );
+my $repeat_consensus = Bio::EnsEMBL::RepeatConsensus->new
+ (-REPEAT_CONSENSUS => 'ACTG',
+ -NAME => 'ACTG(n)',
+ -LENGTH => 4,
+ -REPEAT_CLASS => 'Simple_repeat');
-ok(1);
+#
+# Test new and getters
+#
-my $repeats = $repeat_f_ad->fetch_all_by_RawContig($contig);
+my $start = 10;
+my $end = 100;
+my $strand = -1;
+my $hstart = 1;
+my $hend = 90;
+my $dbID = 123;
+my $score = 12.5;
-my $repeat = $repeats->[0];
+my $rf = Bio::EnsEMBL::RepeatFeature->new
+ (-START => $start,
+ -END => $end,
+ -STRAND => $strand,
+ -ANALYSIS => $analysis,
+ -SLICE => $slice,
+ -HSTART => $hstart,
+ -HEND => $hend,
+ -SCORE => $score,
+ -REPEAT_CONSENSUS => $repeat_consensus);
-ok($repeat);
-ok($repeat->start == 26);
-ok($repeat->hend == 45);
+ok($rf && $rf->isa('Bio::EnsEMBL::RepeatFeature'));
-my $dbID = $repeat->dbID;
+ok($rf->start == $start);
+ok($rf->end == $end);
+ok($rf->strand == $strand);
+ok($rf->analysis == $analysis);
+ok($rf->slice == $slice);
+ok($rf->hstart == $hstart);
+ok($rf->hend == $hend);
+ok($rf->score == $score);
+ok($rf->repeat_consensus == $repeat_consensus);
-my $r = $repeat_f_ad->fetch_by_dbID($dbID);
-ok($r->dbID == $dbID && $r->start == 26 && $r->hend == 45);
+#
+# Test Getter/Setters
+#
+$repeat_consensus = Bio::EnsEMBL::RepeatConsensus->new
+ (-REPEAT_CONSENSUS => 'ACTG',
+ -NAME => 'ACTG(n)',
+ -LENGTH => 4,
+ -REPEAT_CLASS => 'Simple_repeat');
-# list_dbIDs
-my $ids = $repeat_f_ad->list_dbIDs();
-ok (@{$ids});
-$multi->restore('core', 'repeat_feature');
+ok(test_getter_setter($rf, 'hstart', 120));
+ok(test_getter_setter($rf, 'hend', 200));
+ok(test_getter_setter($rf, 'repeat_consensus', $repeat_consensus));
+ok(test_getter_setter($rf, 'score', '45.5'));
+ok($rf->display_id eq 'ACTG(n)');
+ok($rf->hstrand == 1);
diff --git a/modules/t/repeatFeatureAdaptor.t b/modules/t/repeatFeatureAdaptor.t
new file mode 100644
index 0000000000..d8700430e7
--- /dev/null
+++ b/modules/t/repeatFeatureAdaptor.t
@@ -0,0 +1,126 @@
+use strict;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 9;
+}
+
+
+use MultiTestDB;
+use TestUtils qw(test_getter_setter debug);
+
+our $verbose = 0;
+
+
+my $multi = MultiTestDB->new();
+
+my $db = $multi->get_DBAdaptor( 'core' );
+
+my $slice_adaptor = $db->get_SliceAdaptor();
+my $analysis = $db->get_AnalysisAdaptor->fetch_by_logic_name('RepeatMask');
+my $slice = $slice_adaptor->fetch_by_seq_region_id(319456);
+
+my $repeat_f_ad = $db->get_RepeatFeatureAdaptor();
+my $repeat_c_ad = $db->get_RepeatConsensusAdaptor();
+
+
+#
+# Test storing and simple retrieval
+#
+
+my $repeat_consensus = Bio::EnsEMBL::RepeatConsensus->new();
+
+$repeat_consensus->length(10);
+$repeat_consensus->repeat_class('dummy');
+$repeat_consensus->name('dummy');
+$repeat_consensus->repeat_consensus('ATGCATGCAT');
+
+ok($repeat_consensus);
+
+$repeat_c_ad->store($repeat_consensus);
+
+ok(1);
+
+my $repeat_feature = Bio::EnsEMBL::RepeatFeature->new();
+
+$repeat_feature->start(26);
+$repeat_feature->end(65);
+$repeat_feature->strand(1);
+$repeat_feature->hstart(6);
+$repeat_feature->hend(45);
+$repeat_feature->score(100);
+$repeat_feature->analysis($analysis);
+$repeat_feature->repeat_consensus($repeat_consensus);
+$repeat_feature->slice( $slice );
+
+ok($repeat_feature);
+$multi->hide( "core", "repeat_feature" );
+
+$repeat_f_ad->store( $repeat_feature );
+
+
+ok(1);
+
+my $repeats = $repeat_f_ad->fetch_all_by_Slice($slice);
+
+my $repeat = $repeats->[0];
+
+ok($repeat);
+
+ok($repeat->start == 26);
+ok($repeat->hend == 45);
+
+my $dbID = $repeat->dbID;
+
+my $r = $repeat_f_ad->fetch_by_dbID($dbID);
+
+ok($r->dbID == $dbID && $r->start == 26 && $r->hend == 45);
+
+
+#
+# Test list_dbIDs
+#
+my $ids = $repeat_f_ad->list_dbIDs();
+ok (@{$ids});
+
+$multi->restore('core', 'repeat_feature');
+
+
+
+#
+# Test retrieval via Slice
+#
+$slice = $db->get_SliceAdaptor->fetch_by_region('chromosome',
+ '20', 30_000_000,
+ 40_000_000);
+
+my $feats = $repeat_f_ad->fetch_all_by_Slice($slice);
+debug('fetching by chromosomal slice---');
+debug("Got " . scalar(@$feats) . " features back");
+print_features($feats);
+
+
+$r = $repeat_f_ad->fetch_by_dbID(518841);
+$r = $r->transform('supercontig');
+debug('---fetching by dbID and transform to supercontig coords---');
+print_features([$r]);
+
+
+
+sub print_features {
+ my $features = shift;
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ my $seqname = $f->slice->seq_region_name();
+ my $analysis = $f->analysis->logic_name();
+ debug($seqname . ' ' . $f->start().'-'.$f->end().'('.$f->strand().
+ ') ['. $f->dbID.'] '. $f->repeat_consensus->name() . ' ' .
+ $f->hstart .'-'.$f->hend.' '.$f->score() .
+ " ($analysis)");
+ } else {
+ debug('UNDEF');
+ }
+ }
+}
diff --git a/modules/t/seqDumper.t b/modules/t/seqDumper.t
new file mode 100644
index 0000000000..1023f63013
--- /dev/null
+++ b/modules/t/seqDumper.t
@@ -0,0 +1,67 @@
+use lib 't';
+use strict;
+use warnings;
+
+use Bio::EnsEMBL::Utils::SeqDumper;
+use TestUtils qw ( debug test_getter_setter );
+use MultiTestDB;
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 7;
+}
+
+
+our $verbose = 0;
+
+my $multi = MultiTestDB->new();
+
+my $db = $multi->get_DBAdaptor( "core" );
+
+
+my $seq_dumper = Bio::EnsEMBL::Utils::SeqDumper->new();
+
+ok(ref($seq_dumper) && $seq_dumper->isa('Bio::EnsEMBL::Utils::SeqDumper'));
+
+my $file;
+
+if($verbose) {
+ $file = undef;
+} else {
+ $file = '/dev/null';
+}
+
+
+#do not dump snps they are not in core db
+$seq_dumper->disable_feature_type('variation');
+
+my $slice_adaptor = $db->get_SliceAdaptor();
+
+
+my $slice =
+ $slice_adaptor->fetch_by_region('contig', 'AL031658.11.1.162976');
+
+$seq_dumper->dump($slice, 'EMBL', $file);
+ok(1);
+
+$seq_dumper->dump($slice, 'GENBANK', $file);
+ok(1);
+
+$seq_dumper->dump($slice, 'FASTA', $file);
+ok(1);
+
+
+
+
+$slice =
+ $slice_adaptor->fetch_by_region('chromosome', '20', 30_500_000, 30_600_000);
+
+$seq_dumper->dump($slice, 'EMBL', $file);
+ok(1);
+
+$seq_dumper->dump($slice, 'GENBANK', $file);
+ok(1);
+
+$seq_dumper->dump($slice, 'FASTA', $file);
+ok(1);
+
diff --git a/modules/t/sequenceAdaptor.t b/modules/t/sequenceAdaptor.t
new file mode 100644
index 0000000000..4c776e3c7a
--- /dev/null
+++ b/modules/t/sequenceAdaptor.t
@@ -0,0 +1,70 @@
+use strict;
+use warnings;
+
+use lib 't';
+
+BEGIN { $| = 1;
+ use Test;
+ plan tests => 8;
+}
+
+use TestUtils qw( debug );
+
+use MultiTestDB;
+use Bio::EnsEMBL::Slice;
+
+our $verbose= 0;
+
+
+my $multi_db = MultiTestDB->new;
+my $db = $multi_db->get_DBAdaptor('core');
+
+my $CHR = '20';
+my $START = 30_220_000;
+my $END = 31_200_000;
+my $STRAND = 1;
+
+#
+# Test fetch_by_Slice_start_end_strand
+#
+
+my $slice_adaptor = $db->get_SliceAdaptor;
+my $seq_adaptor = $db->get_SequenceAdaptor();
+
+
+my $slice = $slice_adaptor->fetch_by_region('chromosome', $CHR, $START, $END);
+compare_compliments($slice, $seq_adaptor);
+
+$slice = $slice_adaptor->fetch_by_region('clone','AL031658.11');
+compare_compliments($slice, $seq_adaptor);
+
+$slice = $slice_adaptor->fetch_by_region('supercontig','NT_028392');
+compare_compliments($slice, $seq_adaptor);
+
+$slice = $slice_adaptor->fetch_by_region('contig', 'AL031658.11.1.162976');
+compare_compliments($slice, $seq_adaptor);
+
+
+sub compare_compliments {
+ my $slice = shift;
+ my $seq_adaptor = shift;
+
+ my $seq = ${$seq_adaptor->fetch_by_Slice_start_end_strand($slice,1,undef,1)};
+
+ debug('FORWARD STRAND SLICE SEQ for ' . $slice->name());
+ debug($seq);
+
+ my $invert_seq =
+ ${$seq_adaptor->fetch_by_Slice_start_end_strand($slice->invert,1,undef,1)};
+
+ debug('REVERSE STRAND SLICE SEQ for ' . $slice->name());
+ debug($invert_seq);
+
+ ok(length($seq) == $slice->length); #sequence is correct length
+
+ $seq = reverse $seq; #reverse complement seq
+ $seq =~ tr/ACTG/TGAC/;
+
+ ok($seq eq $invert_seq); #revcom same as seq on inverted slice
+}
+
diff --git a/modules/t/simpleFeature.t b/modules/t/simpleFeature.t
index ed10ca4566..918c783a99 100644
--- a/modules/t/simpleFeature.t
+++ b/modules/t/simpleFeature.t
@@ -1,17 +1,19 @@
+use strict;
+
use lib 't';
-use TestUtils qw(test_getter_setter);
+use TestUtils qw(test_getter_setter debug);
BEGIN { $| = 1;
use Test;
- plan tests => 10;
+ plan tests => 34;
}
use MultiTestDB;
use Bio::EnsEMBL::SimpleFeature;
-use Bio::EnsEMBL::RawContig;
-use Bio::Seq;
+our $verbose = 0;
+
my $multi = MultiTestDB->new;
# get a core DBAdaptor
@@ -22,7 +24,7 @@ my $sfa = $dba->get_SimpleFeatureAdaptor;
#
# 1 create a new Simplefeature
#
-$sf = new Bio::EnsEMBL::SimpleFeature;
+my $sf = new Bio::EnsEMBL::SimpleFeature;
ok($sf);
@@ -49,27 +51,6 @@ ok(test_getter_setter($sf,'display_label','dummy_label'));
ok(test_getter_setter($sf,'dbID',42));
-#
-# 8 attach a contig
-#
-# create a dummy seq and contig
-#
-my $seq = Bio::Seq->new(-seq => 'ATGCAGCTAGCATCGATGACATCG',
- -id => 'dummy_contig',
- -accession => 'dummy_contig');
-
-my $contig = Bio::EnsEMBL::RawContig->new();
-
-my $name = 'dummy_contig';
-$contig->id($name);
-$contig->embl_offset(0);
-$contig->seq($seq);
-
-# now attach the contig
-
-$sf->contig($contig);
-ok($sf);
-
#
# 9 check adaptor attaching
@@ -77,6 +58,102 @@ ok($sf);
$sf->adaptor($sfa);
ok($sf->adaptor->isa('Bio::EnsEMBL::DBSQL::SimpleFeatureAdaptor'));
-# list_dbIDs
+
+my $chr_slice = $dba->get_SliceAdaptor->fetch_by_region('chromosome', '20');
+my $features = $sfa->fetch_all_by_Slice($chr_slice);
+debug('--- chr 20 simple features ---');
+debug("Got " . scalar(@$features));
+ok(@$features == 136);
+print_features($features);
+
+
+my $cln_slice = $dba->get_SliceAdaptor->fetch_by_region('clone','AL031658.11');
+$features = $sfa->fetch_all_by_Slice($cln_slice);
+debug('-- cln AL031658.11 simple features ---');
+debug("Got " . scalar(@$features));
+ok(@$features == 20);
+print_features($features);
+
+my $sprctg_slice = $dba->get_SliceAdaptor->fetch_by_region('supercontig',
+ 'NT_028392');
+$features = $sfa->fetch_all_by_Slice($sprctg_slice);
+debug('-- sprctg NT_028392 simple features ---');
+debug("Got " . scalar(@$features));
+ok(@$features == 136);
+print_features($features);
+
+my $ctg_slice = $dba->get_SliceAdaptor->fetch_by_region('contig',
+ 'AL031658.11.1.162976');
+$features = $sfa->fetch_all_by_Slice($ctg_slice);
+debug('--- contig AL031658.11.1.162976 simple features ---');
+debug("Got " . scalar(@$features));
+ok(@$features == 20);
+print_features($features);
+
+
+
+#retrieve a feature via dbID
+
+debug('---- fetch_by_dbID (default coords) ----');
+my $feat = $sfa->fetch_by_dbID(14564);
+ok($feat->dbID == 14564);
+ok($feat->slice->seq_region_name() eq 'AL031658.11.1.162976');
+ok($feat->start == 64109);
+ok($feat->end == 64112);
+ok($feat->strand == 1);
+
+print_features([$feat]);
+
+#transform to chromosome coords
+debug('---- tranform to chromosomal ----');
+$feat = $feat->transform('chromosome');
+ok($feat->dbID == 14564);
+ok($feat->slice->seq_region_name() eq '20');
+ok($feat->start == 30327623);
+ok($feat->end == 30327626);
+ok($feat->strand == 1);
+print_features([$feat]);
+
+# transform to supercontig
+debug('---- tranform to supercontig ----');
+$feat = $feat->transform('contig')->transform('supercontig');
+ok($feat->dbID == 14564);
+ok($feat->slice->seq_region_name() eq 'NT_028392');
+ok($feat->start == 735658);
+ok($feat->end == 735661);
+ok($feat->strand == 1);
+print_features([$feat]);
+
+debug('---- transform to clone ----');
+$feat = $feat->transform('contig')->transform('clone');
+ok($feat->dbID == 14564);
+ok($feat->slice->seq_region_name() eq 'AL031658.11');
+ok($feat->start == 64109);
+ok($feat->end == 64112);
+ok($feat->strand == 1);
+print_features([$feat]);
+
+
+# List_dbidx
my $ids = $sfa->list_dbIDs();
ok (@{$ids});
+
+
+ok($feat->display_id eq $feat->display_label);
+
+
+
+sub print_features {
+ my $features = shift;
+ foreach my $f (@$features) {
+ if(defined($f)) {
+ my $seqname = $f->slice->seq_region_name();
+ my $analysis = $f->analysis->logic_name();
+ debug($seqname . ' ' . $f->start().'-'.$f->end().'('.$f->strand().
+ ') ['. $f->dbID.'] '. $f->display_label.' '.$f->score() .
+ " ($analysis)");
+ } else {
+ debug('UNDEF');
+ }
+ }
+}
diff --git a/modules/t/slice.t b/modules/t/slice.t
index 9624dcc6a8..0a8c4e0095 100644
--- a/modules/t/slice.t
+++ b/modules/t/slice.t
@@ -3,9 +3,9 @@ use warnings;
use lib 't';
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test;
- plan tests => 49;
+ plan tests => 43;
}
use TestUtils qw( debug );
@@ -16,95 +16,104 @@ use Bio::EnsEMBL::Slice;
our $verbose= 0;
#
-#1 TEST - Slice Compiles
+# TEST - Slice Compiles
#
-ok(1);
+ok(1);
my $CHR = '20';
my $START = 30_270_000;
my $END = 31_200_000;
my $STRAND = 1;
-my $ASSEMBLY_TYPE = 'NCBI_30';
-my $DBID = 123;
my $multi_db = MultiTestDB->new;
my $db = $multi_db->get_DBAdaptor('core');
-
#
-#2-5 TEST - Slice creation from adaptor
+# TEST - Slice creation from adaptor
#
my $slice_adaptor = $db->get_SliceAdaptor;
-my $slice = $slice_adaptor->fetch_by_chr_start_end($CHR, $START, $END);
-ok($slice->chr_name eq $CHR);
-ok($slice->chr_start == $START);
-ok($slice->chr_end == $END);
-ok($slice->adaptor);
-
+my $csa = $db->get_CoordSystemAdaptor();
-#
-#6 TEST - Slice::new (empty)
-#
-$slice = new Bio::EnsEMBL::Slice(-empty => 1);
-ok($slice);
+my $slice = $slice_adaptor->fetch_by_region('chromosome', $CHR, $START, $END);
+ok($slice->seq_region_name eq $CHR);
+ok($slice->start == $START);
+ok($slice->end == $END);
+ok($slice->adaptor == $slice_adaptor);
#
-#7-12 TEST - Slice::new
+#TEST - Slice::new
#
-$slice = new Bio::EnsEMBL::Slice(-chr_name => $CHR,
- -chr_start => $START,
- -chr_end => $END,
- -strand => $STRAND,
- -assembly_type => $ASSEMBLY_TYPE,
- -dbid => $DBID);
+my $coord_system = $csa->fetch_by_name('toplevel');
+$slice = new Bio::EnsEMBL::Slice
+ (-seq_region_name => $CHR,
+ -start => $START,
+ -end => $END,
+ -strand => $STRAND,
+ -coord_system => $coord_system);
-ok($slice->chr_name eq $CHR);
-ok($slice->chr_start == $START);
-ok($slice->chr_end == $END);
+ok($slice->seq_region_name eq $CHR);
+ok($slice->start == $START);
+ok($slice->end == $END);
ok($slice->strand == $STRAND);
-ok($slice->assembly_type eq $ASSEMBLY_TYPE);
-ok($slice->dbID == $DBID);
#
-#13 Test - Slice::adaptor
+#Test - Slice::adaptor
#
$slice->adaptor($slice_adaptor);
ok($slice->adaptor == $slice_adaptor);
-#
-#14 Test - Slice::dbID
-#
-$slice->dbID(10);
-ok($slice->dbID==10);
#
-#15-17 Test Slice::name
+#1 Test Slice::name
#
#verify that chr_name start and end are contained in the name
my $name = $slice->name;
-ok($name =~/$CHR/);
-ok($name =~/$START/);
-ok($name =~/$END/);
+ok($name eq "chromosome:NCBI33:$CHR:$START:$END:$STRAND");
+
+#
+# Test Slice::length
+#
+ok($slice->length == ($END-$START + 1));
#
-#18 Test Slice::id
+# Test get_attributes
#
-ok($slice->id eq $slice->name);
+my $clone = $slice_adaptor->fetch_by_region('clone','AL121583.25');
+
+my @types = $clone->get_attribute_types();
+
+ok(@types == 1 && $types[0] eq 'htg_phase');
+
+my @attrib = $clone->get_attribute('htg_phase');
+
+ok(@attrib == 1 && $attrib[0] == 4);
#
-#19 Test Slice::length
+# Test expand
#
-ok($slice->length == ($END-$START + 1));
+my $len = $clone->length();
+
+$clone = $clone->expand(100,100);
+ok(($clone->start == -99) && ($clone->end() == $len+100));
+
+$clone = $clone->expand(-100,-100);
+ok(($clone->start == 1) && ($clone->end() == $len));
+
+$clone = $clone->expand(0,1000);
+ok(($clone->start == 1) && ($clone->end() == $len + 1000));
+
+$clone = $clone->expand(-1000, 0);
+ok(($clone->start == 1001) && ($clone->end() == $len + 1000));
#
-#20-22 Test Slice::invert
+# Test Slice::invert
#
my $inverted_slice = $slice->invert;
ok($slice != $inverted_slice); #slice is not same object as inverted slice
@@ -115,7 +124,7 @@ ok($slice->strand == $STRAND);
#
-# 23-24 Test Slice::seq
+# Test Slice::seq
#
my $seq = uc $slice->seq;
my $invert_seq = uc $slice->invert->seq;
@@ -128,7 +137,7 @@ $seq =~ tr/ACTG/TGAC/;
ok($seq eq $invert_seq); #revcom same as seq on inverted slice
#
-# 25-26 Test Slice::subseq
+# Test Slice::subseq
#
my $SPAN = 10;
my $sub_seq = uc $slice->subseq(-$SPAN,$SPAN);
@@ -142,113 +151,66 @@ $sub_seq =~ tr/ACTG/TGAC/;
ok($sub_seq eq $invert_sub_seq);
#
-# 27 Test Slice::get_all_PredictionTranscripts
+# Test Slice::get_all_PredictionTranscripts
#
my $pts = $slice->get_all_PredictionTranscripts;
-ok(scalar @$pts);
+ok(@$pts == 24);
+#
+# Test Slice::get_seq_region_id
+#
+ok($slice->get_seq_region_id());
#
-# 28 Test Slice::get_all_DnaAlignFeatures
+# Test Slice::get_all_DnaAlignFeatures
#
my $count = 0;
my $dafs = $slice->get_all_DnaAlignFeatures;
-ok(scalar @$dafs);
+ok(@$dafs == 27081);
$count += scalar @$dafs;
#
-# 29 Test Slice::get_all_ProteinAlignFeatures
+# Test Slice::get_all_ProteinAlignFeatures
#
my $pafs = $slice->get_all_ProteinAlignFeatures;
-ok(scalar @$pafs);
+ok(@$pafs == 7205);
$count += scalar @$pafs;
#
-# 30 Test Slice::get_all_SimilarityFeatures
+# Test Slice::get_all_SimilarityFeatures
#
ok($count == scalar @{$slice->get_all_SimilarityFeatures});
#
-# 31 Test Slice::get_all_SimpleFeatures
+# Test Slice::get_all_SimpleFeatures
#
ok(scalar @{$slice->get_all_SimpleFeatures});
#
-# 32 Test Slice::get_all_RepeatFeatures
+# Test Slice::get_all_RepeatFeatures
#
ok(scalar @{$slice->get_all_RepeatFeatures});
#
-# 33 Test Slice::get_all_Genes
+# Test Slice::get_all_Genes
#
ok(scalar @{$slice->get_all_Genes});
#
-# 34 Test Slice::get_all_Genes_by_type
+# Test Slice::get_all_Genes_by_type
#
ok(scalar @{$slice->get_all_Genes_by_type('ensembl')});
-#
-# 35 Test Slice::chr_name
-#
-my $old_val = $slice->chr_name;
-my $new_val = 'Y';
-$slice->chr_name($new_val);
-ok($slice->chr_name eq $new_val);
-$slice->chr_name($old_val);
-
-#
-# 36 Test Slice::chr_start
-#
-$old_val = $slice->chr_start;
-$new_val = 123;
-$slice->chr_start($new_val);
-ok($slice->chr_start == $new_val);
-$slice->chr_start($old_val);
-
-#
-# 37 Test Slice::chr_end
-#
-$old_val = $slice->chr_end;
-$new_val = 1234567;
-$slice->chr_end($new_val);
-ok($slice->chr_end == $new_val);
-$slice->chr_end($old_val);
-#
-# 38 Test Slice::strand
-#
-$old_val = $slice->strand;
-$new_val = $old_val * -1;
-$slice->strand($new_val);
-ok($slice->strand == $new_val);
-$slice->strand($old_val);
#
-# 39 Test Slice::assembly_type
-#
-$old_val = $slice->assembly_type;
-$new_val = 'TEST';
-$slice->assembly_type($new_val);
-ok($slice->assembly_type eq $new_val);
-$slice->assembly_type($old_val);
-
-
-#
-# 40 Test Slice::get_all_KaryotypeBands
+# Test Slice::get_all_KaryotypeBands
#
ok(scalar @{$slice->get_all_KaryotypeBands});
#
-# 41-42 Test Slice::get_Chromosome
-#
-my $chromo;
-ok($chromo = $slice->get_Chromosome);
-ok($chromo->chr_name eq $slice->chr_name);
-
-#
-# 43-44 Test Slice::get_RepeatMaskedSeq
+# Test Slice::get_RepeatMaskedSeq
#
$seq = $slice->seq;
ok(length($slice->get_repeatmasked_seq->seq) == length($seq));
@@ -258,36 +220,35 @@ my $softmasked_seq = $slice->get_repeatmasked_seq(['RepeatMask'], 1)->seq;
ok($softmasked_seq ne $seq);
ok(uc($softmasked_seq) eq $seq);
-$softmasked_seq = $seq = undef;
+$softmasked_seq = $seq = undef;
#
-# 45 Test Slice::get_all_MapFrags
+# Test Slice::get_all_MiscFeatures
#
-# ok(scalar @{$slice->get_all_MapFrags('cloneset')});
+ok(scalar @{$slice->get_all_MiscFeatures()});
#
-# 46 Test Slice::get_tiling_path
+# Test Slice::project
#
-
-ok(scalar @{$slice->get_tiling_path});
+ok(scalar @{$slice->project('seqlevel')});
-my $super_slices = $slice->get_all_supercontig_Slices();
+#my $super_slices = $slice->get_all_supercontig_Slices();
-#
-# 47-48 get_all_supercontig_Slices()
-#
-debug( "Supercontig starts at ".$super_slices->[0]->chr_start() );
+##
+## get_all_supercontig_Slices()
+##
+#debug( "Supercontig starts at ".$super_slices->[0]->chr_start() );
-ok( $super_slices->[0]->chr_start() == 29591966 );
+#ok( $super_slices->[0]->chr_start() == 29591966 );
-debug( "Supercontig name ".$super_slices->[0]->name() );
+#debug( "Supercontig name ".$super_slices->[0]->name() );
-ok( $super_slices->[0]->name() eq "NT_028392" );
+#ok( $super_slices->[0]->name() eq "NT_028392" );
#
-# 49 get_base_count
+# get_base_count
#
my $hash = $slice->get_base_count;
my $a = $hash->{'a'};
@@ -306,3 +267,37 @@ ok($a == 234371
&& $gc_content == 48.59
&& $a+$c+$t+$g+$n == $slice->length);
+my $test_seq = 'ATGCATGCATGCATGCATGCATGC';
+my $test_slice = new Bio::EnsEMBL::Slice
+ (-seq_region_name => 'misc',
+ -start => 1,
+ -end => 24,
+ -strand => 1,
+ -coord_system => $coord_system,
+ -seq => $test_seq,
+ );
+
+
+
+
+
+
+ok($test_slice->length == 24);
+
+$hash = $test_slice->get_base_count;
+$a = $hash->{'a'};
+$c = $hash->{'c'};
+$t = $hash->{'t'};
+$g = $hash->{'g'};
+$n = $hash->{'n'};
+$gc_content = $hash->{'%gc'};
+
+
+
+ok($a == 6
+ && $c == 6
+ && $t == 6
+ && $g == 6
+ && $n == 0
+ && $gc_content == 50
+ && $a+$c+$t+$g+$n == $test_slice->length);
diff --git a/modules/t/sliceAdaptor.t b/modules/t/sliceAdaptor.t
index 4dc7b60fcc..da4c33ffec 100644
--- a/modules/t/sliceAdaptor.t
+++ b/modules/t/sliceAdaptor.t
@@ -5,11 +5,12 @@ use warnings;
BEGIN { $| = 1;
use Test;
- plan tests => 27;
+ plan tests => 50;
}
use MultiTestDB;
use Bio::EnsEMBL::DBSQL::SliceAdaptor;
+use Bio::EnsEMBL::Slice;
use TestUtils qw(test_getter_setter debug);
our $verbose = 0;
@@ -17,7 +18,7 @@ our $verbose = 0;
my ($CHR, $START, $END, $FLANKING) = ("20", 30_252_000, 31_252_001, 1000);
#
-#1 slice adaptor compiles
+# slice adaptor compiles
#
ok(1);
@@ -26,64 +27,58 @@ my $db = $multi->get_DBAdaptor('core');
#
-# 2-3 SliceAdaptor::new
+# SliceAdaptor::new
#
my $slice_adaptor = Bio::EnsEMBL::DBSQL::SliceAdaptor->new($db->_obj);
ok($slice_adaptor->isa('Bio::EnsEMBL::DBSQL::SliceAdaptor'));
ok($slice_adaptor->db);
#
-# 4-6 fetch_by_chr_start_end
+# fetch_by_region
#
-my $slice = $slice_adaptor->fetch_by_chr_start_end($CHR, $START, $END);
-ok($slice->chr_name eq $CHR);
-ok($slice->chr_start == $START);
-ok($slice->chr_end == $END);
+my $slice = $slice_adaptor->fetch_by_region('toplevel',$CHR, $START, $END);
+ok($slice->seq_region_name eq $CHR);
+ok($slice->start == $START);
+ok($slice->end == $END);
#
-# 7-11 fetch_by_contig_name
+# fetch_by_contig_name
#
-my $tiling_path = $slice->get_tiling_path;
+my $projection = $slice->project('seqlevel');
#it is important to get a contig not cut off by slice start or end
-unless(@$tiling_path > 2) {
+unless(@$projection > 2) {
warn("There aren't enough tiles in this path for this test to work");
}
-my $tile = $tiling_path->[1];
-my $contig = $tile->component_Seq;
-my $ctg_start = $tile->component_start;
-my $ctg_end = $tile->component_end;
-$slice = $slice_adaptor->fetch_by_contig_name($contig->name);
+my ($chr_start,$chr_end,$contig) = @{$projection->[1]};
-ok($slice->length == ($ctg_end - $ctg_start + 1));
-ok($slice->seq() eq $contig->subseq($ctg_start, $ctg_end));
-ok($slice->chr_name eq $CHR);
+ok($contig->length == ($chr_end - $chr_start + 1));
-#verify flanking specifier works
-my $new_slice = $slice_adaptor->fetch_by_contig_name($contig->name, $FLANKING);
-ok($new_slice->chr_start == $slice->chr_start - $FLANKING);
-ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
+my $seq1 = $slice->subseq($chr_start, $chr_end);
+my $seq2 = $contig->seq();
+
+ok($seq1 eq $seq2);
#
# 12-13 fetch_by_fpc_name
#
-my $fpc_name = 'NT_011387';
-$slice = $slice_adaptor->fetch_by_supercontig_name($fpc_name);
-ok($new_slice->chr_start);
-ok($new_slice->chr_end);
+#my $fpc_name = 'NT_011387';
+#$slice = $slice_adaptor->fetch_by_supercontig_name($fpc_name);
+#ok($new_slice->chr_start);
+#ok($new_slice->chr_end);
#
# 14 - 15 fetch_by_clone_accession
#
-my $clone_acc = 'AL031658';
-$slice = $slice_adaptor->fetch_by_clone_accession($clone_acc);
-$new_slice = $slice_adaptor->fetch_by_clone_accession($clone_acc, $FLANKING);
-ok($new_slice->chr_start == $slice->chr_start - $FLANKING);
-ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
+#my $clone_acc = 'AL031658';
+#$slice = $slice_adaptor->fetch_by_clone_accession($clone_acc);
+#$new_slice = $slice_adaptor->fetch_by_clone_accession($clone_acc, $FLANKING);
+#ok($new_slice->chr_start == $slice->chr_start - $FLANKING);
+#ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
#
@@ -91,11 +86,11 @@ ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
#
my $t_stable_id = 'ENST00000217315';
$slice = $slice_adaptor->fetch_by_transcript_stable_id($t_stable_id);
-$new_slice = $slice_adaptor->fetch_by_transcript_stable_id($t_stable_id,
- $FLANKING);
+my $new_slice = $slice_adaptor->fetch_by_transcript_stable_id($t_stable_id,
+ $FLANKING);
-ok($new_slice->chr_start == $slice->chr_start - $FLANKING);
-ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
+ok($new_slice->start == $slice->start - $FLANKING);
+ok($new_slice->end == $slice->end + $FLANKING);
#
@@ -105,8 +100,8 @@ my $transcript = $db->get_TranscriptAdaptor->fetch_by_stable_id($t_stable_id);
my $tid = $transcript->dbID;
$slice = $slice_adaptor->fetch_by_transcript_id($tid);
$new_slice = $slice_adaptor->fetch_by_transcript_id($tid, $FLANKING);
-ok($new_slice->chr_start == $slice->chr_start - $FLANKING);
-ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
+ok($new_slice->start == $slice->start - $FLANKING);
+ok($new_slice->end == $slice->end + $FLANKING);
#
@@ -115,8 +110,8 @@ ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
my $g_stable_id = 'ENSG00000125964';
$slice = $slice_adaptor->fetch_by_gene_stable_id($g_stable_id);
$new_slice = $slice_adaptor->fetch_by_gene_stable_id($g_stable_id, $FLANKING);
-ok($new_slice->chr_start == $slice->chr_start - $FLANKING);
-ok($new_slice->chr_end == $slice->chr_end + $FLANKING);
+ok($new_slice->start == $slice->start - $FLANKING);
+ok($new_slice->end == $slice->end + $FLANKING);
#verify we can retrieve the gene from this slice
my $gene_found = 0;
@@ -139,22 +134,231 @@ foreach my $g (@{$new_slice->get_all_Genes}) {
ok($gene_found);
+
+#
+# fetch_by_region (entire region)
+#
+$slice = $slice_adaptor->fetch_by_region('chromosome',$CHR);
+ok($slice->seq_region_name eq $CHR);
+ok($slice->start == 1);
+
+
+#
+# fetch_by_misc_feature_attribute
+#
+my $flanking= 1000;
+$slice = $slice_adaptor->fetch_by_misc_feature_attribute('superctg',
+ 'NT_030871',
+ $flanking);
+
+ok($slice->seq_region_name eq '20');
+ok($slice->start == 59707812 - $flanking);
+ok($slice->end == 60855021 + $flanking);
+
+
+#
+# normalized projected slice
+#
+
+#
+# a slice with a PAR region
+# 24,25
+#
+$slice = $slice_adaptor->fetch_by_region( "chromosome", "Y", 9_000_000, 11_000_000, 1 );
+
+my $results = $slice_adaptor->fetch_normalized_slice_projection( $slice );
+
+debug( "Pseudo autosomal region results" );
+for my $projection ( @$results ) {
+ debug( "Start: ".$projection->[0] );
+ debug( "End: ".$projection->[1] );
+ debug( "Slice ".$projection->[2] );
+ debug( "-----------" );
+}
+
+ok( @$results == 3 );
+ok( $results->[1]->[2]->seq_region_name() eq "20" );
+
#
-# 24-26 fetch_by_chr_name
+# a slice with a haplotype
+# 26,27
#
-$slice = $slice_adaptor->fetch_by_chr_name($CHR);
-ok($slice->chr_name eq $CHR);
-ok($slice->chr_start == 1);
-my $chromo = $db->get_ChromosomeAdaptor->fetch_by_chr_name($CHR);
-ok($chromo->length eq $slice->chr_end);
+$slice = $slice_adaptor->fetch_by_region( "chromosome", "20_HAP1", 30_000_000, 31_000_000, 1 );
+$results = $slice_adaptor->fetch_normalized_slice_projection( $slice );
+
+debug( "Haplotype projection results" );
+for my $projection ( @$results ) {
+ debug( "Start: ".$projection->[0] );
+ debug( "End: ".$projection->[1] );
+ debug( "Slice ".$projection->[2] );
+ debug( "-----------" );
+}
+
+ok( @$results == 3 );
+ok( $results->[0]->[2]->seq_region_name() eq "20" );
+ok( $results->[1]->[2]->seq_region_name() eq "20_HAP1" );
+ok( $results->[2]->[2]->seq_region_name() eq "20" );
-$slice = $slice_adaptor->fetch_by_chr_start_end("20", 29_252_000, 31_252_001 );
-my $name_list = $slice_adaptor->list_overlapping_supercontigs( $slice );
-for my $name ( @$name_list ) {
- debug( "Overlapping supercontig ".$name );
+#try a projection from chromosome 20 to supercontigs
+$slice = $slice_adaptor->fetch_by_region('chromosome', "20", 29_252_000,
+ 31_252_001 );
+
+debug("Projection from chromosome 20 to supercontig");
+my @projection = @{$slice->project('supercontig')};
+ok(@projection == 1);
+ok($projection[0]->[2]->seq_region_name eq 'NT_028392');
+foreach my $seg (@projection) {
+ my ($start, $end, $slice) = @$seg;
+ debug("$start-$end " . $slice->seq_region_name);
}
-ok( grep { $_ eq "NT_028392" } @$name_list);
+#try a projection from clone to supercontig
+$slice = $slice_adaptor->fetch_by_region('clone', 'AL121583.25');
+
+debug("Projection from clone AL121583.25 to supercontig");
+
+@projection = @{$slice->project('supercontig')};
+ok(@projection == 1);
+ok($projection[0]->[2]->seq_region_name eq 'NT_028392');
+foreach my $seg (@projection) {
+ my ($start, $end, $slice) = @$seg;
+ debug("$start-$end -> " . $slice->start . '-'. $slice->end . ' ' . $slice->seq_region_name);
+}
+
+#
+# test storing a couple of different slices
+#
+my $csa = $db->get_CoordSystemAdaptor();
+my $cs = $csa->fetch_by_name('contig');
+
+$multi->save('core', 'seq_region', 'dna', 'dnac');
+
+my $len = 50;
+my $name = 'testregion';
+
+#
+# Store a slice with sequence
+#
+
+$slice = Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $cs,
+ -SEQ_REGION_NAME => $name,
+ -START => 1,
+ -END => $len,
+ -STRAND => 1);
+
+
+my $seq = 'A' x $len;
+
+
+
+$slice_adaptor->store($slice, \$seq);
+$slice = $slice_adaptor->fetch_by_region('contig', $name);
+
+ok($slice->length == $len);
+ok($slice->seq eq $seq);
+ok($slice->seq_region_name eq $name);
+
+#
+# Store a slice without sequence
+#
+
+$cs = $csa->fetch_by_name('chromosome');
+
+$len = 50e6;
+$name = 'testregion2';
+$slice = Bio::EnsEMBL::Slice->new(-COORD_SYSTEM => $cs,
+ -SEQ_REGION_NAME => $name,
+ -START => 1,
+ -END => $len,
+ -STRAND => 1);
+
+$slice_adaptor->store($slice);
+
+$slice = $slice_adaptor->fetch_by_region('chromosome', $name);
+ok($slice->length eq $len);
+ok($slice->seq_region_name eq $name);
+
+$multi->restore('core', 'seq_region', 'dna', 'dnac');
+
+
+#
+# There was a bug such that features were not being retrieved
+# from slices that had a start < 1. This is a test for that case.
+#
+$slice = $slice_adaptor->fetch_by_region('chromosome', '20', 1,35_000_000);
+debug("slice start = " . $slice->start);
+debug("slice end = " . $slice->end);
+
+my $sfs1 = $slice->get_all_SimpleFeatures();
+print_features($sfs1);
+
+$slice = $slice_adaptor->fetch_by_region('chromosome', '20', -10, 35_000_000);
+
+debug("slice start = " . $slice->start);
+debug("slice end = " . $slice->end);
+
+my $sfs2 = $slice->get_all_SimpleFeatures();
+print_features($sfs2);
+
+ok(@$sfs1 == @$sfs2);
+
+
+#
+# test fetch_by_name
+#
+$slice = $slice_adaptor->fetch_by_name($slice->name());
+
+ok($slice->coord_system->name eq 'chromosome');
+ok($slice->seq_region_name eq '20');
+ok($slice->start == -10);
+ok($slice->strand == 1);
+ok($slice->end == 35e6);
+
+$slice = $slice_adaptor->fetch_by_name('clone::AL121583.25:1:10000:-1');
+
+ok($slice->coord_system->name eq 'clone');
+ok($slice->seq_region_name eq 'AL121583.25');
+ok($slice->start == 1);
+ok($slice->end == 10000);
+ok($slice->strand == -1);
+
+
+#
+# test fetch_all
+#
+my $slices = $slice_adaptor->fetch_all('chromosome');
+ok(@$slices == 62);
+
+$slices = $slice_adaptor->fetch_all('chromosome', undef,1e6, 1e4);
+
+print_slices($slices);
+ok(@$slices == 3185);
+
+
+$slices = $slice_adaptor->fetch_all('contig', undef, 50000);
+
+ok(@$slices == 26);
+
+print_slices($slices);
+
+
+sub print_slices {
+ my $slices = shift;
+ foreach my $slice (@$slices) {
+ debug($slice->name());
+ }
+ debug( "Got ". scalar(@$slices));
+}
+
+sub print_features {
+ my $fs = shift;
+ foreach my $f (@$fs) {
+ my $start = $f->start();
+ my $end = $f->end();
+ my $strand = $f->strand();
+ debug(" $start-$end($strand)");
+ }
+}
diff --git a/modules/t/test_genome.zip b/modules/t/test_genome.zip
index b41ff6a8ef91b61663e878241dcab507c8dda0ef..e41f7098604573e28a0df4297bb829144d5ea43a 100644
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zjwJC#oJ`$|9x9-q@c&o%oK3IDtQF`s9+|feSk2Y+a-My<FW!brzohlHz=*1}@u>Yn
zAj^P&L&2k={|V-S&u}W)*ua;;(kxdaU}9k-YI8vho~0MW!v0YONz%cg2>ux$K`a}D
I3r>gs0cT1%bpQYW
diff --git a/modules/t/transcript.t b/modules/t/transcript.t
index 82af890406..1c2dfd3548 100644
--- a/modules/t/transcript.t
+++ b/modules/t/transcript.t
@@ -3,9 +3,9 @@ use strict;
use warnings;
use vars qw( $verbose );
-BEGIN { $| = 1;
+BEGIN { $| = 1;
use Test;
- plan tests => 31;
+ plan tests => 38;
}
use MultiTestDB;
@@ -24,7 +24,7 @@ my $db = $multi->get_DBAdaptor('core' );
my $sa = $db->get_SliceAdaptor();
-my $slice = $sa->fetch_by_chr_start_end("20", 30_249_935, 31_254_640 );
+my $slice = $sa->fetch_by_region('chromosome', "20", 30_249_935, 31_254_640 );
my $genes = $slice->get_all_Genes();
@@ -46,8 +46,8 @@ for my $gene ( @$genes ) {
}
}
- $gene->transform();
-
+ $gene = $gene->transform( "contig" );
+ next if( ! $gene );
for my $trans ( @{$gene->get_all_Transcripts()} ) {
if( $trans->translate()->seq() =~ /\*./ ) {
$translates = 0;
@@ -61,6 +61,30 @@ debug( "utr Transcript is $utr_trans" );
ok( $translates );
+#
+# Try pulling off genes from an NTContig and making sure they still translate.
+# This is a fairly good test of the chained coordinate mapping since the
+# transcripts are stored in chromosomal coordinates and there is no direct
+# mapping path to the supercontig coordinate system.
+#
+my $supercontig = $sa->fetch_by_region('supercontig', "NT_028392");
+
+my $transcripts = $supercontig->get_all_Transcripts();
+
+debug( "Checking if all transcripts on NTContig NT_028392 translate and transform" );
+
+$translates = 1;
+for my $trans ( @$transcripts ) {
+ if( $trans->translate()->seq() =~ /\*./ ) {
+ $translates = 0;
+ debug( $trans->stable_id()." does not translate." );
+ last;
+ }
+ debug($trans->stable_id() . ":" . $trans->translate()->seq() . "\n");
+}
+
+ok($translates);
+
my $ta = $db->get_TranscriptAdaptor();
@@ -74,10 +98,12 @@ ok (@{$stable_ids});
my $tr = $ta->fetch_by_stable_id( "ENST00000217347" );
+$tr = $tr->transform('contig');
+
ok( $tr );
debug ( "External transcript name: " . $tr->external_name );
-ok ( $tr->external_name eq "MAPRE1");
+ok ( $tr->external_name eq "MAPRE1");
debug ( "External transcript dbname: " . $tr->external_db );
ok ( $tr->external_db eq 'HUGO' );
@@ -173,6 +199,8 @@ ok( scalar( @{$tr->get_all_Exons()} ) == 0 );
# get a fresh tr to check the update method
$tr = $ta->fetch_by_stable_id( "ENST00000217347" );
+$multi->save('core', 'transcript');
+
# the first update should have no effect
$ta->update($tr);
@@ -187,4 +215,45 @@ $ta->update($tr);
$up_tr = $ta->fetch_by_stable_id( "ENST00000217347" );
ok ( $up_tr->display_xref->dbID() == 614 );
+$multi->restore('core', 'transcript');
+
+
+
+my $interpro = $ta->get_Interpro_by_transid("ENST00000252021");
+foreach my $i (@$interpro) {
+ debug($i);
+}
+###currently no interpro info in the test db
+ok(@$interpro == 1);
+
+#
+# test fetch_all_by_external_name
+#
+
+($tr) = @{$ta->fetch_all_by_external_name('BAB15482')};
+ok($tr && $tr->stable_id eq 'ENST00000262651');
+
+#
+# test fetch_by_translation_id
+#
+
+$tr = $ta->fetch_by_translation_id(21734);
+
+ok($tr && $tr->stable_id eq 'ENST00000201961');
+
+ok($tr->display_id() eq $tr->stable_id());
+
+#
+# regression test: five_prime_utr and three_prime_utr were failing
+# for transcripts that had no UTR. undef should have been returned instead
+#
+$tr = $ta->fetch_by_stable_id('ENST00000246203');
+
+my $three_prime = $tr->three_prime_utr();
+
+ok(!defined($three_prime));
+
+my $five_prime = $tr->five_prime_utr();
+
+ok(!defined($five_prime));
diff --git a/modules/t/translation.t b/modules/t/translation.t
index d92088dd32..1702b4f58c 100644
--- a/modules/t/translation.t
+++ b/modules/t/translation.t
@@ -1,17 +1,18 @@
use lib 't';
-use TestUtils qw(test_getter_setter);
+use TestUtils qw(debug test_getter_setter);
use Bio::EnsEMBL::Translation;
use Bio::EnsEMBL::Exon;
BEGIN { $| = 1;
use Test;
- plan tests => 11;
+ plan tests => 19;
}
my $loaded = 0;
END {print "not ok 1\n" unless $loaded;}
-my $verbose = 0;
+#turn on/off debug prints:
+our $verbose = 0;
use MultiTestDB;
@@ -28,7 +29,7 @@ my $t = Bio::EnsEMBL::Translation->new();
ok($t);
ok(test_getter_setter($t,'stable_id',1));
-ok(test_getter_setter($t,'temporary_id',2));
+
ok(test_getter_setter($t,'dbID',3));
ok(test_getter_setter($t,'start',42));
@@ -39,7 +40,6 @@ $exon->start(10);
$exon->end(20);
$exon->strand(1);
$exon->phase(0);
-#$exon->contig( $contig );
$exon->end_phase( -1 );
$t->start_Exon($exon);
@@ -48,6 +48,11 @@ ok($t);
$t->end_Exon($exon);
ok($t);
+
+#
+# Tests for the translation adaptor
+##################################
+
my $ta = $db->get_TranslationAdaptor();
my $ids = $ta->list_dbIDs();
ok (@{$ids});
@@ -55,3 +60,54 @@ ok (@{$ids});
my $stable_ids = $ta->list_stable_ids();
ok (@{$stable_ids});
+
+my $tra = $db->get_TranscriptAdaptor();
+
+my $transcript = $tra->fetch_by_stable_id('ENST00000201961');
+
+#
+# test fetch_by_Transcript
+#
+my $translation = $ta->fetch_by_Transcript($transcript);
+
+ok($translation && $translation->stable_id eq 'ENSP00000201961');
+ok($translation && $translation->start_Exon->stable_id eq 'ENSE00000661216');
+ok($translation && $translation->end_Exon->stable_id eq 'ENSE00000661212');
+
+
+#
+# test fetch_by_dbID
+#
+$translation = $ta->fetch_by_dbID(21734);
+ok($translation && $translation->stable_id() eq 'ENSP00000201961');
+
+
+#
+# test fetch_by_stable_id
+#
+$translation = $ta->fetch_by_stable_id('ENSP00000201961');
+ok($translation && $translation->dbID() == 21734);
+
+#
+# test fetch_by_external_name
+#
+($translation) = @{$ta->fetch_all_by_external_name('CAC33959')};
+ok($translation && $translation->dbID() == 21716);
+
+#
+# test get_all_ProteinFeatures
+#
+
+my @protein_features = @{$translation->get_all_ProteinFeatures()};
+debug("Got " . scalar(@protein_features) ." protein features.");
+ok(@protein_features == 3);
+
+
+#
+# test get_all_DomainFeatures
+#
+my @domain_features = @{$translation->get_all_DomainFeatures()};
+debug("Got " . scalar(@domain_features) . " domain features.");
+ok(@domain_features == 3);
+
+ok($translation->display_id eq $translation->stable_id);
\ No newline at end of file
diff --git a/scripts/dna_compress.pl b/scripts/dna_compress.pl
index a545c0033c..3dcd5104f0 100755
--- a/scripts/dna_compress.pl
+++ b/scripts/dna_compress.pl
@@ -15,26 +15,22 @@
use strict;
use Getopt::Std;
-use vars qw($opt_h $opt_T $opt_i
- $opt_U $opt_D $opt_P $opt_H $opt_p
- $opt_s $opt_c $opt_C $opt_l $opt_d $opt_r $opt_n);
-
-use Time::HiRes qw(gettimeofday);
+use vars qw($opt_H $opt_T
+ $opt_u $opt_d $opt_P $opt_h $opt_p $opt_C $opt_U);
use Bio::EnsEMBL::DBSQL::DBAdaptor;
+use Bio::EnsEMBL::Utils::Exception qw(throw warning);
-getopts("hTi:U:D:P:H:p:s:cCl:dr:n:");
+getopts("u:HTd:p:P:h:CU");
$|=1;
# specify defaults
-my $def_U='ensadmin';
-my $def_D='testdna';
-my $def_P='3307';
-my $def_H='127.0.0.1';
-my $def_r=5;
+my $def_u='ensadmin';
+my $def_P='3306';
+my $def_h='127.0.0.1';
-if($opt_h){
+if($opt_H){
&help;
}elsif($opt_T){
&help2;
@@ -42,23 +38,20 @@ if($opt_h){
sub help {
print <<ENDHELP;
-dna_compress.pl
- -h for help
- -s char string of DNA (for writing)
- -l char label of clone, contig (for writing)
- -c convert
- -C compare
- -r num number of randomisation subsequence selections [$def_r]
- -i id clone_dbid (for comparing, converting, extracting DNA)
- -d delete clone
- -n num process N items
-
- -U user ensembl db user [$def_U]
- -D db ensembl db [$def_D]
+usage:
+dna_compress.pl [options]
+
+ -H for help
+ -T tutorial
+ -d database
+ -u user ensembl db user [$def_u]
+ -d db ensembl db
-P port port of mysql [$def_P]
- -H host host of mysql [$def_H]
+ -h host host of mysql [$def_h]
-p pass passwd for mysqluser
+ -C compress
+ -U uncompress
ENDHELP
exit 0;
@@ -69,268 +62,176 @@ sub help2 {
}
# defaults or options
-$opt_U=$def_U unless $opt_U;
-$opt_D=$def_D unless $opt_D;
-$opt_P=$def_P unless $opt_P;
-$opt_H=$def_H unless $opt_H;
-$opt_r=$def_r unless $opt_r;
+$opt_u ||= $def_u;
+$opt_h ||= $def_h;
+$opt_P ||= $def_P unless $opt_P;
-# db connection
-my $db = new Bio::EnsEMBL::DBSQL::DBAdaptor(-host => $opt_H,
- -user => $opt_U,
- -port => $opt_P,
- -dbname => $opt_D,
- -pass => $opt_p);
-my $seq_apt=$db->get_SequenceAdaptor;
-my $contig_apt=$db->get_RawContigAdaptor;
-my $clone_apt=$db->get_CloneAdaptor;
+if(!$opt_d) {
+ print STDERR "ensembl db (-d) argument is required\n";
+ help();
+}
-if($opt_C || $opt_c){
-
- # convert dna of sequences (loop over clone->contig->dna table,
- # inserting into dnac table when no entry is already present)
- # OR
- # compare raw and compressed sequences
-
- my $clones=$clone_apt->fetch_all();
- my $nd=0;
- my $ns=0;
- my $nc=0;
- my $tc=0;
- my $tu=0;
- foreach my $clone (@$clones){
- my $clone_dbid=$clone->dbID;
- next if($opt_i && $opt_i!=$clone_dbid);
- my $clone_id=$clone->id;
- my $created=$clone->created();
- print "Processing Clone $clone_id ($clone_dbid) $created\n";
- my $contigs=$clone->get_all_Contigs();
- foreach my $contig (@$contigs){
- my $contig_dbid=$contig->dbID;
- my $contig_id=$clone->id;
- my $seq=$seq_apt->fetch_by_RawContig_start_end_strand($contig,1,-1,1);
- my $len=length($seq);
-
- my $dna_id;
- # custom sql is required to get dna_id and check if there is a compressed id
- my $query="SELECT c.dna_id FROM contig c WHERE c.contig_id = ?";
- my $sth=$contig_apt->prepare($query);
- $sth->execute($contig_dbid);
- if(my $aref=$sth->fetchrow_arrayref()){
- ($dna_id)=@$aref;
- }else{
- $contig_apt->thrown("No dna_id for Contig $contig_id");
- }
-
- print " Processing Contig $contig_id ($contig_dbid) [$len] $dna_id\n";
-
- my $flag_compressed_exists=0;
- $query="SELECT d.dna_id FROM dnac d WHERE d.dna_id = ?";
- $sth=$seq_apt->prepare($query);
- $sth->execute($dna_id);
- if(my $aref=$sth->fetchrow_arrayref()){
- $flag_compressed_exists=1;
- }
- # convert
- if($opt_c){
- if($flag_compressed_exists){
- print " Compressed DNA entry already exists for dna_id $dna_id\n";
- next;
- }
- # write compressed DNA record
- my $id=$seq_apt->store_compressed($seq,$created,$dna_id);
- print " Created compressed DNA entry\n";
- }elsif($opt_C){
- if(!$flag_compressed_exists){
- print " Compressed DNA entry missing for dna_id $dna_id\n";
- next;
- }
- my $seq2=$seq_apt->fetch_by_RawContig_start_end_strand($contig,1,-1,1,1);
- if($seq ne $seq2){
- print "DIFFERENT\n";
- $nd++;
- }else{
- print "Same\n";
- $ns++;
- }
- my $len2=length($seq2);
- my $lenx=30;
- if($len<$lenx){
- $lenx=$len;
- print substr($seq,0,$lenx)."\n".substr($seq2,0,$lenx)."\n\n";
- }else{
- print substr($seq,0,$lenx)." .. ".substr($seq,$len-$lenx,$len)."\n";
- print substr($seq2,0,$lenx)." .. ".substr($seq2,$len-$lenx,$len2)."\n\n";
- }
-
- # now test a subsequence
- my $lenr=$len-1;
- for(my $i=0;$i<$opt_r;$i++){
- my $r=rand;
- my $st=int($r*$lenr)+1;
- $r=rand;
- my $ed=int($r*($lenr-$st))+$st+1;
- print "$lenr: $st-$ed\n";
- my $t1=gettimeofday;
- $seq=$seq_apt->fetch_by_RawContig_start_end_strand($contig,$st,$ed,1);
- $tu+=gettimeofday-$t1;
- $len=length($seq);
- $t1=gettimeofday;
- $seq2=$seq_apt->fetch_by_RawContig_start_end_strand($contig,$st,$ed,1,1);
- $tc+=gettimeofday-$t1;
- if($seq ne $seq2){
- print "DIFFERENT\n";
- $nd++;
- }else{
- print "Same\n";
- $ns++;
- }
- my $len2=length($seq2);
- my $lenx=30;
- if($len<$lenx){
- $lenx=$len;
- print substr($seq,0,$lenx)."\n".substr($seq2,0,$lenx)."\n\n";
- }else{
- print substr($seq,0,$lenx)." .. ".substr($seq,$len-$lenx,$len)."\n";
- print substr($seq2,0,$lenx)." .. ".substr($seq2,$len-$lenx,$len2)."\n\n";
- }
- }
- }
-
- }
- $nc++;
- last if($opt_n && $nc>$opt_n);
- }
- print "Same: $ns; Different: $nd\n" if $opt_C;
- my $ratio=0;
- $ratio=$tc/$tu unless $tu==0;
- printf "Uncompressed time: %5.1f; Compressed time: %5.1f (%5.1f)\n",$tu,$tc,$ratio;
- exit 0;
-
-}elsif($opt_s && $opt_l){
-
- # sequence and label defined, so write into db;
-
- my $acc=$opt_l;
- my $seq=$opt_s;
- my $ver=1;
- # clone object
- my $clone=new Bio::EnsEMBL::Clone;
- $clone->id($acc);
- $clone->htg_phase(4);
- $clone->embl_id($acc);
- $clone->version($ver);
- $clone->embl_version($ver);
- my $now = time;
- $clone->created($now);
- $clone->modified($now);
-
- # contig object
- my $contig = new Bio::EnsEMBL::RawContig;
- my $length = length($seq);
- $contig->name("$acc.$ver.1.$length");
- $contig->embl_offset(1);
- $contig->length($length);
- $contig->seq($seq);
- $clone->add_Contig($contig);
-
- my $dbclone;
- eval {
- $dbclone = $clone_apt->fetch_by_accession_version($acc,$ver);
- };
- if($dbclone){
- #$dbclone->delete_by_dbID;
- $clone_apt->remove($dbclone);
- print "remove old version of clone\n";
- }
- my $id=$clone_apt->store($clone);
+if(!$opt_C && !$opt_U) {
+ print STDERR "either compress (-C) or uncompress (-U) is required\n";
+ help();
+}
+
+
+# db connection
+my $db = new Bio::EnsEMBL::DBSQL::DBAdaptor(-host => $opt_h,
+ -user => $opt_u,
+ -port => $opt_P,
+ -dbname => $opt_d,
+ -pass => $opt_p);
- print "Clone '$acc' stored under dbid $id\n";
-
- exit 0;
-}elsif($opt_d && $opt_i){
- # remove clone
+my $meta_container = $db->get_MetaContainer();
+my $comp_seq_adaptor = $db->get_CompressedSequenceAdaptor();
- my $clone=$clone_apt->fetch_by_dbID($opt_i);
- if($clone ne ''){
- $clone_apt->remove($clone);
- print "Clone $opt_i removed\n";
- }else{
- print "Clone not found\n";
+my ($compressed) =
+ @{$meta_container->list_value_by_key('sequence.compression')};
+
+if($opt_C) {
+ #compress the dna in the database
+ if($compressed) {
+ throw("The database meta table indicates this database already contains"
+ ." sequence compression.\nIf this is not the case do the following:\n"
+ ." mysql> delete from meta where meta_key = 'sequence.compression'\n");
}
-}elsif($opt_i){
+ #one more sanity check - make sure that dna table is populated and dnac table
+ #is not
+ if(get_dna_count($db) == 0) {
+ print STDERR "There is no uncompressed dna in this database to compress.";
+ exit(0);
+ }
+ if(get_dnac_count($db) != 0) {
+ throw("Unexpected: the dnac table already contains some rows.\n");
+ }
+
+ print STDERR "Compressing sequence and writing to dnac table.\n";
+
+ #get every slice that contains sequence
+ my $slice_adaptor = $db->get_SliceAdaptor();
+ foreach my $slice (@{$slice_adaptor->fetch_all('seqlevel')}) {
+ my $seq_region_id = $slice_adaptor->get_seq_region_id($slice);
+ $comp_seq_adaptor->store($seq_region_id, $slice->seq);
+ print STDERR ".";
+ }
- foreach my $id (split(/,/,$opt_i)){
- my $clone=$clone_apt->fetch_by_dbID($id);
- my $contigs=$clone->get_all_Contigs;
- foreach my $contig (@$contigs){
- my $cid=$contig->dbID;
- print "$cid: ".$contig->seq."\n";
- }
+ print STDERR "\nSetting sequence.compression meta table entry.\n";
+ $meta_container->delete_key('sequence.compression');
+ $meta_container->store_key_value('sequence.compression', 1);
+
+ print STDERR "Deleting uncompressed sequence.\n";
+ my $sth = $db->prepare("delete from dna");
+ $sth->execute();
+
+ print STDERR "All done.\n";
+
+} else {
+ #uncompress the dna in the database
+ if(!$compressed) {
+ throw("The database meta table indicates that the sequence in this "
+ ." database is already uncompressed.\nIf this is not the case do "
+ ." the following:\n"
+ ." mysql> delete from meta where meta_key = 'sequence.compression';\n"
+ ." mysql> insert into meta (meta_key,meta_value) "
+ . "values('sequence.compression', 1);\n");
}
- exit 0;
-}else{
+ if(get_dnac_count($db) == 0) {
+ print STDERR "There is no compressed dna in this database to uncompress.";
+ exit(0);
+ }
+ if(get_dna_count($db) != 0) {
+ throw("Unexpected: the dna table already contains some rows.\n");
+ }
- print "WARN - provide a valid contig_id (reading) or sequence to write\n";
+ print STDERR "Setting sequence.compression meta table entry.\n";
+ $meta_container->delete_key('sequence.compression');
+ $meta_container->store_key_value('sequence.compression', 0);
-}
+ print STDERR "Uncompressing sequence and writing to dna table.\n";
-__END__
+ #get every slice that contains sequence
+ my $slice_adaptor = $db->get_SliceAdaptor();
+ my $seq_adaptor = $db->get_SequenceAdaptor();
+
+ if($seq_adaptor->isa('CompressedSequenceAdaptor')) {
+ throw("Tried to get SequenceAdaptor but got CompressedSequenceAdaptor.");
+ }
-=pod
+ foreach my $slice (@{$slice_adaptor->fetch_all('seqlevel')}) {
+ my $seq_region_id = $slice_adaptor->get_seq_region_id($slice);
+ my $seq = $comp_seq_adaptor->fetch_by_Slice_start_end_strand($slice);
+ $seq_adaptor->store($seq_region_id, $$seq);
+ print STDERR ".";
+ }
-=head1 NAME - name
+ print STDERR "\nDeleting compressed sequence.\n";
+ my $sth = $db->prepare("delete from dnac");
+ $sth->execute();
-dna_compress.pl
+ print STDERR "All done.\n";
+}
-=head1 DESCRIPTION
-Populates and tests the experimental dnac (compressed dna) table of ensembl.
+sub get_dna_count {
+ my $db = shift;
-=head1 SYNOPSIS
+ my $sth = $db->prepare("SELECT count(*) from dna");
+ $sth->execute();
+ my ($count) = $sth->fetchrow_array();
+ $sth->finish();
-
+ return $count;
+}
-=head1 EXAMPLES
-Create a clone/clontig/dna entry
+sub get_dnac_count {
+ my $db = shift;
- dna_compress.pl -s 'ACGTTTGGAANANGCCGTTNNNNACCGNGTGCTAAGCC' -l test
+ my $sth = $db->prepare("SELECT count(*) from dnac");
+ $sth->execute();
+ my ($count) = $sth->fetchrow_array();
+ $sth->finish();
+
+ return $count;
+}
-Clone 'test' stored under dbid 42
-Create a compressed version of this entry
+__END__
- dna_compress.pl -c -i 42
+=pod
+=head1 NAME - name
-Compare compressed and uncompressed versions of this entry
+dna_compress.pl
- dna_compress.pl -C -i 42
+=head1 DESCRIPTION
-(carries out a fetch of the entire contig and then 5 different random parts from it)
+Converts compressed sequence in an ensembl database to uncompressed sequence
+or converts uncompressed sequence in an ensembl database to compressed
+sequence.
+=head1 SYNOPSIS
-Extract sequence from DB, autosensing when only compressed is present.
+
- dna_compress.pl -i 42
+=head1 EXAMPLES
-28: ACGTTTGGAANANGCCGTTNNNNACCGNGTGCTAAGCC
+Compress the contents of the dna table in an ensembl database:
- mysql> delete from dna where dna_id=42;
+ dna_compress.pl -C -u ensadmin -p password -h host -d homo_sapiens_core_18_34
- dna_compress.pl -i 42
+Uncompress the contents of the dnac table in an ensembl database
-Switched to compressed DNA reading mode
-28: ACGTTTGGAANANGCCGTTNNNNACCGNGTGCTAAGCC
+ dna_compress.pl -U -u ensadmin -p password -h host -d homo_sapiens_core_18_34
-
=head1 FLAGS
@@ -344,25 +245,29 @@ Displays short help
Displays this help message
-=item -s <string>
+=item -C
-String of DNA to write into database
+Compress the dna in the database
-=item -l <string>
+=item -U
-Label for clonename, contigname to write into database
+Uncompress the dna in the database
-=item -c
+=item -h
-Make compressed DNA entry for all clones in DB, or just those listed by dbid (-i)
+The database host
-=item -C
+=item -p
+
+The database password
-Compare uncompressed and compressed DNA entries where present, and/or listed by dbid (-i)
+=item -P
-=item -i <num[,num]>
+The database port
-List of clone dbid
+=item -u
+
+The database user
=back
@@ -374,6 +279,10 @@ List of clone dbid
B<th> initial release
+=item 08-Oct-2003
+
+B<mcvicker> rewrote for new schema and newly implemented sequence compression
+
=back
=head1 BUGS
diff --git a/sql/table.sql b/sql/table.sql
index 888e6d3150..ef8c509fff 100755
--- a/sql/table.sql
+++ b/sql/table.sql
@@ -1,40 +1,34 @@
# revisited schema naming issues
# Author: Arne Stabenau
# Date: 12.11.2001
-
-# conventions
-# use lower case and underscores
-# internal ids are integers named tablename_id
-# same name is given in foreign key relations
-
-
-#
-# Table structure for table 'analysis'
#
+# Glenn Proctor July 2003 - adapted for new schema structure
#
+# Note that more information about each table can be found in
+# ensembl/docs/schema_description/
-CREATE TABLE analysis (
- analysis_id int(10) unsigned DEFAULT '0' NOT NULL auto_increment,
- created datetime DEFAULT '0000-00-00 00:00:00' NOT NULL,
- logic_name varchar(40) not null,
- db varchar(120),
- db_version varchar(40),
- db_file varchar(120),
- program varchar(80),
- program_version varchar(40),
- program_file varchar(80),
- parameters varchar(255),
- module varchar(80),
- module_version varchar(40),
- gff_source varchar(40),
- gff_feature varchar(40),
+# Conventions:
+# - use lower case and underscores
+# - internal ids are integers named tablename_id
+# - same name is given in foreign key relations
- PRIMARY KEY (analysis_id),
- KEY logic_name_idx( logic_name )
+
+CREATE TABLE alt_allele (
+ alt_allele_id INT NOT NULL auto_increment,
+ gene_id INT NOT NULL,
+
+ UNIQUE gene_idx( gene_id ),
+ UNIQUE allele_idx( alt_allele_id, gene_id )
);
+
+
-# semantics
+################################################################################
+#
+# Table structure for table 'analysis'
+#
+# semantics:
# analysis_id - internal id
# created - date to distinguish newer and older versions off the
# same analysis. Not well maintained so far.
@@ -52,491 +46,511 @@ CREATE TABLE analysis (
# - how to make a gff dump from features with this analysis
-#
-# Table structure for table 'chromosome'
-#
-CREATE TABLE chromosome (
- chromosome_id int unsigned NOT NULL auto_increment,
- name varchar(40) NOT NULL,
- length int(11) NULL,
-
- PRIMARY KEY (chromosome_id),
- unique name (name)
-);
-
+CREATE TABLE analysis (
-#
-# Table structure for table 'clone'
-#
-CREATE TABLE clone (
- clone_id int(10) unsigned NOT NULL auto_increment,
- name varchar(40) NOT NULL,
- embl_acc varchar(40) NOT NULL,
- version int(10) NOT NULL,
- embl_version int(10) NOT NULL,
- htg_phase int(10) DEFAULT '-1' NOT NULL,
- created datetime NOT NULL,
- modified datetime NOT NULL,
-
- PRIMARY KEY (clone_id),
- KEY embl (embl_acc,embl_version),
- KEY id (name, version)
-);
+ analysis_id int(10) unsigned DEFAULT '0' NOT NULL auto_increment,
+ created datetime DEFAULT '0000-00-00 00:00:00' NOT NULL,
+ logic_name varchar(40) not null,
+ db varchar(120),
+ db_version varchar(40),
+ db_file varchar(120),
+ program varchar(80),
+ program_version varchar(40),
+ program_file varchar(80),
+ parameters varchar(255),
+ module varchar(80),
+ module_version varchar(40),
+ gff_source varchar(40),
+ gff_feature varchar(40),
-# semantics
-# id - string we give to this clone in ensembl
-# should be same as embl_id unless clone is not in embl
-# embl_id - hows the clone submitted to embl
-# htg_phase - finished/unfinished: draft is 123, finished is 4
+ PRIMARY KEY (analysis_id),
+ KEY logic_name_idx( logic_name )
+);
+################################################################################
#
# Table structure for table 'map_density'
#
CREATE TABLE map_density (
- chromosome_id int unsigned NOT NULL,
- chr_start int(10) NOT NULL,
- chr_end int(10) NOT NULL,
- type varchar(20) NOT NULL,
- value int(10) NOT NULL,
+
+ seq_region_id int unsigned NOT NULL,
+ seq_region_start int(10) NOT NULL,
+ seq_region_end int(10) NOT NULL,
+ type varchar(20) NOT NULL,
+ value int(10) NOT NULL,
- PRIMARY KEY(type,chromosome_id,chr_start)
-);
+ PRIMARY KEY(type,seq_region_id,seq_region_start)
-#
-# Table structure for table 'contig'
-#
-CREATE TABLE contig (
- contig_id int(10) unsigned NOT NULL auto_increment,
- name varchar(40) NOT NULL,
- clone_id int(10) NOT NULL,
- length int(10) unsigned NOT NULL, # foreign key clone:internal_id
- embl_offset int(10) unsigned,
- dna_id int(10) NOT NULL, # foreign key dna:id
-
- PRIMARY KEY (contig_id),
- UNIQUE name (name),
- KEY clone (clone_id),
- KEY dna (dna_id)
);
-
-
+################################################################################
#
# Table structure for table 'dna'
#
-
-# This table holds the sequence of the contigs from the contig table.
-# The sequence is that of the contig, not that of the golden
-# path. I.e. to construct the golden path from the dna entries,
-# the sequence of contigs with an orientation of -1 must be
-# reversed and bases complemented. The assembly
-# table has the contig orientation (raw_ori).
-# Note the length of the dna.sequence field is always equal
-# to the appropriate length field in the contig table
-# (probably a violation of some form of normal form since contig.length
-# is an attibute of the dna.sequence field)
+# This table stores DNA sequence.
CREATE TABLE dna (
- dna_id int(10) unsigned NOT NULL auto_increment,
- sequence mediumtext NOT NULL,
- created datetime NOT NULL,
-
- PRIMARY KEY (dna_id)
+ seq_region_id int unsigned NOT NULL,
+ sequence mediumtext NOT NULL,
+
+ PRIMARY KEY (seq_region_id)
+
) MAX_ROWS = 750000 AVG_ROW_LENGTH = 19000;
+################################################################################
#
# Table structure for table 'dnac'
#
-
# Contains equivalent data to dna table, but 4 letters of DNA code are represented
# by a single binary character, based on 2 bit encoding
-# don't need to worry about ambiguity of length, since this is stored in contig.length
+# do not need to worry about ambiguity of length, since this is stored in contig.length
# n_line column contains start-end pairs of coordinates in the string that are really Ns
CREATE TABLE dnac (
- dna_id int(10) unsigned NOT NULL auto_increment,
+ seq_region_id int unsigned NOT NULL,
sequence mediumblob NOT NULL,
- created datetime NOT NULL,
n_line text,
- PRIMARY KEY (dna_id)
+ PRIMARY KEY (seq_region_id)
) MAX_ROWS = 750000 AVG_ROW_LENGTH = 19000;
+################################################################################
#
# Table structure for table 'exon'
#
-
-# Note seq_start always less that seq_end, i.e.
-# when the exon is on the other strand the seq_start
-# is specifying the 3' end of the exon.
-
-# The Sticky Rank differentiates between fragments of
-# the same exon. I.e for exons that
-# span multiple contigs, all the fragments
-# are in this table with the same id,
-# but different sticky_rank values
+# Note seq_region_start always less that seq_region_end, i.e.
+# when the exon is on the other strand the seq_region_start
+# is specifying the 3prime end of the exon.
CREATE TABLE exon (
- exon_id int unsigned NOT NULL auto_increment,
- contig_id int(10) unsigned NOT NULL, # foreign key, contig:internal_id
- contig_start int(10) unsigned NOT NULL, # start of exon within contig
- contig_end int(10) unsigned NOT NULL, # end of exon within specified contig
- contig_strand tinyint(2) NOT NULL, # 1 or -1 depending on the strand of the exon
-
- phase tinyint(2) NOT NULL,
- end_phase tinyint(2) NOT NULL,
- sticky_rank tinyint DEFAULT '1' NOT NULL, # see note above
+
+ exon_id int unsigned NOT NULL auto_increment,
+ seq_region_id int(10) unsigned NOT NULL, # foreign key, seq_region:seq_region_id
+ seq_region_start int(10) unsigned NOT NULL, # start of exon within seq_region
+ seq_region_end int(10) unsigned NOT NULL, # end of exon within specified seq_region
+ seq_region_strand tinyint(2) NOT NULL, # 1 or -1 depending on the strand of the exon
+
+ phase tinyint(2) NOT NULL,
+ end_phase tinyint(2) NOT NULL,
- PRIMARY KEY ( exon_id, sticky_rank),
- KEY contig_idx (contig_id, contig_start )
-);
+ PRIMARY KEY (exon_id),
+ KEY seq_region_idx (seq_region_id, seq_region_start )
-CREATE TABLE exon_stable_id (
- exon_id int unsigned not null, # foreign key exon:exon_id
- stable_id VARCHAR(40) not null,
- version int(10),
- created datetime NOT NULL,
- modified datetime NOT NULL,
-
- PRIMARY KEY( exon_id ),
- UNIQUE( stable_id, version )
);
+################################################################################
+#
+# Table structure for table 'exon_stable_id'
+#
+CREATE TABLE exon_stable_id (
+
+ exon_id int unsigned not null, # foreign key exon:exon_id
+ stable_id VARCHAR(128) not null,
+ version int(10),
+
+ PRIMARY KEY( exon_id ),
+ UNIQUE( stable_id, version )
+);
+
+################################################################################
#
# Table structure for table 'exon_transcript'
#
+# Note that the rank column indicates the 5prime to 3prime position of the
+# exon within the transcript ie rank of 1 means the exon is the 5prime most
+# within this transcript
+
CREATE TABLE exon_transcript (
- exon_id INT unsigned NOT NULL, # foreign key exon:exon_id
- transcript_id INT unsigned NOT NULL, # foregin key transcript:transcript_id
- rank int(10) NOT NULL, # Indicates the 5' to 3' position of the exon
- # within the transcript ie rank of 1 means
- # the exon is the 5' most within this transcript
-
+
+ exon_id INT unsigned NOT NULL, # foreign key exon:exon_id
+ transcript_id INT unsigned NOT NULL, # foregin key transcript:transcript_id
+ rank int(10) NOT NULL,
+
PRIMARY KEY (exon_id,transcript_id,rank),
KEY transcript (transcript_id),
KEY exon ( exon_id )
-);
+);
+################################################################################
+#
+# Table structure for table 'simple_feature'
+#
CREATE TABLE simple_feature (
- simple_feature_id int unsigned not null auto_increment,
- contig_id int(10) unsigned NOT NULL,
- contig_start int(10) unsigned NOT NULL,
- contig_end int(10) unsigned NOT NULL,
- contig_strand tinyint(1) NOT NULL,
- display_label varchar(40) NOT NULL, # what to show, may link to other things, depends on analysis
- analysis_id int(10) unsigned NOT NULL,
-# What scoring do we need ?
-
- score double,
+ simple_feature_id int unsigned not null auto_increment,
+ seq_region_id int(10) unsigned NOT NULL,
+ seq_region_start int(10) unsigned NOT NULL,
+ seq_region_end int(10) unsigned NOT NULL,
+ seq_region_strand tinyint(1) NOT NULL,
+ display_label varchar(40) NOT NULL, # what to show, may link to other things, depends on analysis
+ analysis_id int(10) unsigned NOT NULL,
+ score double,
PRIMARY KEY ( simple_feature_id ),
- KEY contig_idx( contig_id ),
+ KEY seq_region_idx (seq_region_id, seq_region_start ),
KEY analysis_idx( analysis_id ),
KEY hit_idx( display_label )
+
) MAX_ROWS=100000000 AVG_ROW_LENGTH=80;
+################################################################################
+#
+# Table structure for table 'protein_align_feature'
+#
CREATE TABLE protein_align_feature (
- protein_align_feature_id int unsigned not null auto_increment,
- contig_id int(10) unsigned NOT NULL,
- contig_start int(10) unsigned NOT NULL,
- contig_end int(10) unsigned NOT NULL,
- contig_strand tinyint(1) DEFAULT '1' NOT NULL,
- hit_start int(10) NOT NULL,
- hit_end int(10) NOT NULL,
- hit_name varchar(40) NOT NULL,
- analysis_id int(10) unsigned NOT NULL,
-
- # What scoring do we need ?
-
- score double,
- evalue double,
- perc_ident float,
- cigar_line text,
-
- PRIMARY KEY ( protein_align_feature_id ),
- KEY hit_idx( hit_name ),
- KEY ctg_idx( contig_id ),
- KEY ana_idx( analysis_id )
+
+ protein_align_feature_id int unsigned not null auto_increment,
+ seq_region_id int(10) unsigned NOT NULL,
+ seq_region_start int(10) unsigned NOT NULL,
+ seq_region_end int(10) unsigned NOT NULL,
+ seq_region_strand tinyint(1) DEFAULT '1' NOT NULL,
+ hit_start int(10) NOT NULL,
+ hit_end int(10) NOT NULL,
+ hit_name varchar(40) NOT NULL,
+ analysis_id int(10) unsigned NOT NULL,
+ score double,
+ evalue double,
+ perc_ident float,
+ cigar_line text,
+
+ PRIMARY KEY ( protein_align_feature_id ),
+ KEY seq_region_idx( seq_region_id, analysis_id, score, seq_region_start ),
+ KEY hit_idx( hit_name )
+
) MAX_ROWS=100000000 AVG_ROW_LENGTH=80;
+################################################################################
+#
+# Table structure for table 'dna_align_feature'
+#
CREATE TABLE dna_align_feature (
- dna_align_feature_id int unsigned not null auto_increment,
- contig_id int(10) unsigned NOT NULL,
- contig_start int(10) unsigned NOT NULL,
- contig_end int(10) unsigned NOT NULL,
- contig_strand tinyint(1) NOT NULL,
- hit_start int NOT NULL,
- hit_end int NOT NULL,
- hit_strand tinyint(1) NOT NULL,
- hit_name varchar(40) NOT NULL,
- analysis_id int(10) unsigned NOT NULL,
-
-# What scoring do we need ?
-
- score double,
- evalue double,
- perc_ident float,
- cigar_line text,
+
+ dna_align_feature_id int unsigned not null auto_increment,
+ seq_region_id int(10) unsigned NOT NULL,
+ seq_region_start int(10) unsigned NOT NULL,
+ seq_region_end int(10) unsigned NOT NULL,
+ seq_region_strand tinyint(1) NOT NULL,
+ hit_start int NOT NULL,
+ hit_end int NOT NULL,
+ hit_strand tinyint(1) NOT NULL,
+ hit_name varchar(40) NOT NULL,
+ analysis_id int(10) unsigned NOT NULL,
+ score double,
+ evalue double,
+ perc_ident float,
+ cigar_line text,
PRIMARY KEY ( dna_align_feature_id ),
- KEY hit_idx( hit_name ),
- KEY ctg_idx( contig_id ),
- KEY ana_idx( analysis_id )
+ KEY seq_region_idx( seq_region_id, analysis_id, score, seq_region_start ),
+ KEY hit_idx( hit_name )
+
) MAX_ROWS=100000000 AVG_ROW_LENGTH=80;
+################################################################################
+#
+# Table structure for table 'repeat_consensus'
+#
+CREATE TABLE repeat_consensus (
+ repeat_consensus_id int unsigned NOT NULL auto_increment,
+ repeat_name varchar(255) NOT NULL,
+ repeat_class varchar(40) NOT NULL, # eg: SINE, LINE, DNA Transposon,
+ # Retroviral LTR, Satellite,Tandem
+ repeat_consensus text,
+
+ PRIMARY KEY( repeat_consensus_id ),
+ KEY name (repeat_name),
+ KEY class (repeat_class),
+ KEY consensus(repeat_consensus(10))
-CREATE TABLE repeat_consensus (
- repeat_consensus_id int unsigned NOT NULL auto_increment,
- repeat_name varchar(255) NOT NULL,
- repeat_class varchar(40) NOT NULL, # eg: SINE, LINE, DNA Transposon,
- # Retroviral LTR, Satellite,Tandem
- repeat_consensus text, # Or dna_id with entry in DNA table?
-
- PRIMARY KEY( repeat_consensus_id ),
- KEY name (repeat_name),
- KEY class (repeat_class),
- KEY consensus(repeat_consensus(10))
);
+################################################################################
+#
+# Table structure for table 'repeat_feature'
+#
CREATE TABLE repeat_feature (
- repeat_feature_id int unsigned NOT NULL auto_increment,
- contig_id int(10) unsigned NOT NULL,
- contig_start int(10) unsigned NOT NULL,
- contig_end int(10) unsigned NOT NULL,
- contig_strand tinyint(1) DEFAULT '1' NOT NULL,
- repeat_start int(10) NOT NULL,
- repeat_end int(10) NOT NULL,
- repeat_consensus_id int(10) unsigned NOT NULL,
- analysis_id int(10) unsigned NOT NULL,
-
-# What scoring do we need ?
-
- score double,
+
+ repeat_feature_id int unsigned NOT NULL auto_increment,
+ seq_region_id int(10) unsigned NOT NULL,
+ seq_region_start int(10) unsigned NOT NULL,
+ seq_region_end int(10) unsigned NOT NULL,
+ seq_region_strand tinyint(1) DEFAULT '1' NOT NULL,
+ repeat_start int(10) NOT NULL,
+ repeat_end int(10) NOT NULL,
+ repeat_consensus_id int(10) unsigned NOT NULL,
+ analysis_id int(10) unsigned NOT NULL,
+ score double,
PRIMARY KEY ( repeat_feature_id ),
- KEY contig_idx( contig_id ),
+ KEY seq_region_idx( seq_region_id, seq_region_start ),
KEY repeat_idx( repeat_consensus_id ),
KEY analysis_idx( analysis_id )
+
) MAX_ROWS=100000000 AVG_ROW_LENGTH=80;
+################################################################################
#
# Table structure for table 'gene'
#
+
CREATE TABLE gene (
- gene_id int unsigned NOT NULL auto_increment,
- type VARCHAR(40) NOT NULL,
- analysis_id int,
- transcript_count int NOT NULL,
- display_xref_id int unsigned NOT NULL,
+
+ gene_id int unsigned NOT NULL auto_increment,
+ type VARCHAR(40) NOT NULL,
+ analysis_id int,
+ seq_region_id int(10) unsigned NOT NULL,
+ seq_region_start int(10) unsigned NOT NULL,
+ seq_region_end int(10) unsigned NOT NULL,
+ seq_region_strand tinyint(2) NOT NULL,
+ display_xref_id int unsigned,
PRIMARY KEY (gene_id),
+ KEY seq_region_idx( seq_region_id, seq_region_start ),
KEY xref_id_index ( display_xref_id )
-);
+);
+################################################################################
+#
+# Table structure for table 'gene_stable_id'
+#
CREATE TABLE gene_stable_id (
- gene_id int unsigned not null, # foreign key gene:gene_id
- stable_id VARCHAR(40) not null,
- version int(10),
- created datetime NOT NULL,
- modified datetime NOT NULL,
-
- PRIMARY KEY( gene_id ),
- UNIQUE( stable_id, version )
+
+ gene_id int unsigned not null, # foreign key gene:gene_id
+ stable_id VARCHAR(128) not null,
+ version int(10),
+
+ PRIMARY KEY( gene_id ),
+ UNIQUE( stable_id, version )
+
);
+################################################################################
#
# Table structure for table 'supporting_feature'
#
CREATE TABLE supporting_feature (
- exon_id int(11) DEFAULT '0' NOT NULL,
- feature_type enum('dna_align_feature','protein_align_feature'),
- feature_id int(11) DEFAULT '0' NOT NULL,
+
+ exon_id int(11) DEFAULT '0' NOT NULL,
+ feature_type enum('dna_align_feature','protein_align_feature'),
+ feature_id int(11) DEFAULT '0' NOT NULL,
+
UNIQUE all_idx (exon_id,feature_type,feature_id),
KEY feature_idx (feature_type,feature_id)
-) MAX_ROWS=100000000 AVG_ROW_LENGTH=80;
-
-
+) MAX_ROWS=100000000 AVG_ROW_LENGTH=80;
+################################################################################
#
# Table structure for table 'transcript'
#
+
CREATE TABLE transcript (
- transcript_id INT UNSIGNED NOT NULL auto_increment,
- gene_id INT UNSIGNED NOT NULL, # foreign key gene:gene_id
- translation_id INT UNSIGNED NOT NULL, # foreign key translation:translation_id
- exon_count int NOT NULL,
- display_xref_id int unsigned NOT NULL,
+
+ transcript_id INT UNSIGNED NOT NULL auto_increment,
+ gene_id INT UNSIGNED NOT NULL, # foreign key gene:gene_id
+ seq_region_id int(10) unsigned NOT NULL,
+ seq_region_start int(10) unsigned NOT NULL,
+ seq_region_end int(10) unsigned NOT NULL,
+ seq_region_strand tinyint(2) NOT NULL,
+ display_xref_id int unsigned,
PRIMARY KEY (transcript_id),
+ KEY seq_region_idx( seq_region_id, seq_region_start ),
KEY gene_index (gene_id),
- KEY translation_index ( translation_id ),
KEY xref_id_index ( display_xref_id )
);
+################################################################################
+#
+# Table structure for table 'transcript_stable_id'
+#
+
CREATE TABLE transcript_stable_id (
- transcript_id int unsigned not null, # foreign key transcript:transcript_id
- stable_id VARCHAR(40) not null,
- version int(10),
-
- PRIMARY KEY( transcript_id ),
- UNIQUE( stable_id, version )
-);
+ transcript_id int unsigned not null, # foreign key transcript:transcript_id
+ stable_id VARCHAR(128) not null,
+ version int(10),
+
+ PRIMARY KEY( transcript_id ),
+ UNIQUE( stable_id, version )
+);
+
+################################################################################
#
# Table structure for table 'translation'
#
-
-# The seq_start and seq_end are 1-based offsets into the
-# *relative* coordinate system of start_exon_id and end_exon_id.
-# ie, if the translation starts at the first base of the exon, seq_start
-# would be 1
+# The seq_start and seq_end are 1-based offsets into the *relative* coordinate
+# system of start_exon_id and end_exon_id. i.e. if the translation starts at the
+# first base of the exon, seq_start would be 1
CREATE TABLE translation (
- translation_id INT UNSIGNED NOT NULL auto_increment,
- seq_start INT(10) NOT NULL, # relative to exon start
- start_exon_id INT UNSIGNED NOT NULL, # foreign key exon:exon_id
- seq_end INT(10) NOT NULL, # relative to exon start
- end_exon_id INT UNSIGNED NOT NULL, # foreign key exon:exon_id
+
+ translation_id INT UNSIGNED NOT NULL auto_increment,
+ transcript_id INT UNSIGNED NOT NULL,
+ seq_start INT(10) NOT NULL, # relative to exon start
+ start_exon_id INT UNSIGNED NOT NULL, # foreign key exon:exon_id
+ seq_end INT(10) NOT NULL, # relative to exon start
+ end_exon_id INT UNSIGNED NOT NULL, # foreign key exon:exon_id
- PRIMARY KEY (translation_id)
+ PRIMARY KEY (translation_id),
+ KEY (transcript_id)
);
+
+################################################################################
+#
+# Table structure for table 'translation_stable_id'
+#
CREATE TABLE translation_stable_id (
- translation_id INT unsigned NOT NULL, # foreign key translation:translation_id
- stable_id VARCHAR(40) NOT NULL,
- version INT(10),
-
- PRIMARY KEY( translation_id ),
- UNIQUE( stable_id, version )
+
+ translation_id INT unsigned NOT NULL, # foreign key translation:translation_id
+ stable_id VARCHAR(128) NOT NULL,
+ version INT(10),
+
+ PRIMARY KEY( translation_id ),
+ UNIQUE( stable_id, version )
+
);
-# this is a denormalised golden path
+################################################################################
+#
+# Table structure for table 'assembly'
#
+# This is a denormalised golden path.
# The data in this table defines the "static golden path", i.e. the
# best effort draft full genome sequence as determined by the UCSC or NCBI
# (depending which assembly you are using)
#
-# Each row represents a contig (raw_id, FK from contig table) at least part of
-# which is present in the golden path. The part of the contig that is
-# in the path is delimited by fields raw_start and raw_end (start < end), and
-# the absolute position within the golden path chromosome (chromosome_id) is given
-# by chr_start and chr_end. Each contig is in some "supercontig" such as a
-# "fingerprint clone contig" or NT contig and the super contig is identified
-# by field superctg_name and the position of the specified bit of the contig
-# within its super contig is given by fields superctg_start and superctg_end.
-# With the data set at time of this writing, field type is always "NCBI_xx".
-#
-# NB, chr_start <= chr_end, raw_start <= raw_end, and superctg_start <= superctg_end.
+# Each row represents a component, e.g. a contig, (comp_seq_region_id,
+# FK from seq_region table) at least part of which is present in the golden path.
+# The part of the component that is in the path is delimited by fields cmp_start
+# and cmp_end (start < end), and the absolute position within the golden path
+# chromosome (or other appropriate assembled structure) (asm_seq_region_id) is
+# given by asm_start and asm_end.
#
-
CREATE TABLE assembly (
- chromosome_id int unsigned NOT NULL,
- chr_start int(10) NOT NULL,
- chr_end int(10) NOT NULL,
- superctg_name varchar(20) NOT NULL,
- superctg_start int(10) NOT NULL,
- superctg_end int(10) NOT NULL,
- superctg_ori tinyint(2) NOT NULL,
- contig_id int(10) unsigned NOT NULL, # foreign key contig:internal_id
- contig_start int(10) NOT NULL,
- contig_end int(10) NOT NULL,
- contig_ori tinyint NOT NULL,
- type varchar(20) NOT NULL,
-
- PRIMARY KEY(contig_id,type),
- KEY(superctg_name, superctg_start),
- KEY(chromosome_id,chr_start)
-);
+ asm_seq_region_id int unsigned NOT NULL,
+ cmp_seq_region_id int(10) unsigned NOT NULL,
+ asm_start int(10) NOT NULL,
+ asm_end int(10) NOT NULL,
+ cmp_start int(10) NOT NULL,
+ cmp_end int(10) NOT NULL,
+ ori tinyint NOT NULL,
+
+ KEY(cmp_seq_region_id),
+ KEY(asm_seq_region_id, asm_start)
+);
+
+################################################################################
#
# Table structure for table 'protein_feature'
#
CREATE TABLE protein_feature (
- protein_feature_id int(10) unsigned NOT NULL auto_increment,
- translation_id int NOT NULL,
- seq_start int(10) NOT NULL,
- seq_end int(10) NOT NULL,
- hit_start int(10) NOT NULL,
- hit_end int(10) NOT NULL,
- hit_id varchar(40) NOT NULL,
- analysis_id int(10) unsigned NOT NULL,
- score double NOT NULL,
- evalue double,
- perc_ident float,
+
+ protein_feature_id int(10) unsigned NOT NULL auto_increment,
+ translation_id int NOT NULL,
+ seq_start int(10) NOT NULL,
+ seq_end int(10) NOT NULL,
+ hit_start int(10) NOT NULL,
+ hit_end int(10) NOT NULL,
+ hit_id varchar(40) NOT NULL,
+ analysis_id int(10) unsigned NOT NULL,
+ score double NOT NULL,
+ evalue double,
+ perc_ident float,
PRIMARY KEY (protein_feature_id),
KEY (translation_id),
KEY hid_index ( hit_id )
+
);
+################################################################################
#
-#Table structure for table 'interpro'
+# Table structure for table 'interpro'
#
CREATE TABLE interpro (
- interpro_ac varchar(40) NOT NULL,
- id varchar(40) NOT NULL,
+
+ interpro_ac varchar(40) NOT NULL,
+ id varchar(40) NOT NULL,
+
KEY (interpro_ac),
KEY (id)
-);
+);
+################################################################################
#
-#Table structure for table gene_description
+# Table structure for table 'gene_description'
#
CREATE TABLE gene_description (
- gene_id int unsigned NOT NULL,
- description text,
+
+ gene_id int unsigned NOT NULL,
+ description text,
PRIMARY KEY (gene_id)
+
);
+################################################################################
+#
+# Table structure for table 'karyotype'
+#
+
CREATE TABLE karyotype (
- chromosome_id int unsigned NOT NULL,
- chr_start int(10) NOT NULL,
- chr_end int(10) NOT NULL,
- band varchar(40) NOT NULL,
- stain varchar(40) NOT NULL,
- PRIMARY KEY (chromosome_id,band)
-);
+ karyotype_id int unsigned NOT NULL auto_increment,
+ seq_region_id int unsigned NOT NULL,
+ seq_region_start int(10) NOT NULL,
+ seq_region_end int(10) NOT NULL,
+ band varchar(40) NOT NULL,
+ stain varchar(40) NOT NULL,
+ PRIMARY KEY (karyotype_id),
+ KEY region_band_idx (seq_region_id,band)
+);
+
+################################################################################
#
-#Table structure for table objectXref
+# Table structure for table 'object_xref'
#
-CREATE TABLE object_xref(
- object_xref_id INT not null auto_increment,
- ensembl_id int unsigned not null,
- ensembl_object_type ENUM( 'RawContig', 'Transcript', 'Gene', 'Translation' ) not null,
- xref_id INT unsigned not null,
+CREATE TABLE object_xref (
+
+ object_xref_id INT not null auto_increment,
+ ensembl_id int unsigned not null,
+ ensembl_object_type ENUM( 'RawContig', 'Transcript', 'Gene', 'Translation' ) not null,
+ xref_id INT unsigned not null,
+
+ UNIQUE ( ensembl_object_type, ensembl_id, xref_id ),
+ KEY oxref_idx( object_xref_id, xref_id, ensembl_object_type, ensembl_id ),
+ KEY xref_idx(xref_id, ensembl_object_type)
- UNIQUE ( ensembl_object_type, ensembl_id, xref_id ),
- KEY xref_index( object_xref_id, xref_id, ensembl_object_type, ensembl_id )
);
+################################################################################
#
-#Table structure for identity_xref
+# Table structure for table 'identity_xref'
#
CREATE TABLE identity_xref(
@@ -544,8 +558,8 @@ CREATE TABLE identity_xref(
query_identity int(5),
target_identity int(5),
- query_start int,
- query_end int,
+ hit_start int,
+ hit_end int,
translation_start int,
translation_end int,
cigar_line text,
@@ -557,310 +571,547 @@ CREATE TABLE identity_xref(
PRIMARY KEY (object_xref_id)
);
+################################################################################
+#
+# Table structure for table 'go_xref'
+#
CREATE TABLE go_xref (
+
object_xref_id int(10) unsigned DEFAULT '0' NOT NULL,
linkage_type enum('IC', 'IDA', 'IEA', 'IEP', 'IGI', 'IMP',
'IPI', 'ISS', 'NAS', 'ND', 'TAS', 'NR') NOT NULL,
KEY (object_xref_id),
UNIQUE(object_xref_id, linkage_type)
-);
+);
+################################################################################
#
-#Table structure for table xref
+# Table structure for table 'xref'
#
CREATE TABLE xref (
- xref_id INT unsigned not null auto_increment,
- external_db_id int not null,
- dbprimary_acc VARCHAR(40) not null,
- display_label VARCHAR(40) not null,
- version VARCHAR(10) DEFAULT '' NOT NULL,
- description VARCHAR(255),
- PRIMARY KEY( xref_id ),
- UNIQUE KEY id_index( dbprimary_acc, external_db_id ),
- KEY display_index ( display_label )
-);
+ xref_id INT unsigned not null auto_increment,
+ external_db_id int not null,
+ dbprimary_acc VARCHAR(40) not null,
+ display_label VARCHAR(40) not null,
+ version VARCHAR(10) DEFAULT '' NOT NULL,
+ description VARCHAR(255),
+ PRIMARY KEY( xref_id ),
+ UNIQUE KEY id_index( dbprimary_acc, external_db_id ),
+ KEY display_index ( display_label )
+);
+
+################################################################################
#
-#Table structure for table externalSynonym
+# Table structure for table 'external_synonym'
#
-CREATE TABLE external_synonym(
- xref_id INT unsigned not null,
- synonym VARCHAR(40) not null,
- PRIMARY KEY( xref_id, synonym ),
- KEY name_index( synonym )
- );
+CREATE TABLE external_synonym (
-#
-#Table structure for table externalDB
-#
+ xref_id INT unsigned not null,
+ synonym VARCHAR(40) not null,
+ PRIMARY KEY( xref_id, synonym ),
+ KEY name_index( synonym )
-CREATE TABLE external_db(
- external_db_id INT not null,
- db_name VARCHAR(100) NOT NULL,
- release VARCHAR(40) NOT NULL,
- status ENUM ('KNOWNXREF','KNOWN','XREF','PRED','ORTH', 'PSEUDO') not null,
- PRIMARY KEY( external_db_id )
);
+################################################################################
+#
+# Table structure for table 'external_db'
+#
+CREATE TABLE external_db (
-CREATE TABLE meta (
- meta_id INT not null auto_increment,
- meta_key varchar( 40 ) not null,
- meta_value varchar( 255 ) not null,
+ external_db_id INT not null,
+ db_name VARCHAR(100) NOT NULL,
+ release VARCHAR(40) NOT NULL,
+ status ENUM ('KNOWNXREF','KNOWN','XREF','PRED','ORTH', 'PSEUDO') not null,
- PRIMARY KEY( meta_id ),
- KEY meta_key_index ( meta_key ),
- KEY meta_value_index ( meta_value )
-);
+ PRIMARY KEY( external_db_id )
+);
-# Auto add schema version to database
-insert into meta (meta_key, meta_value) values ("schema_version", "$Revision$");
-CREATE TABLE prediction_transcript (
- prediction_transcript_id int unsigned not null auto_increment,
+CREATE TABLE prediction_exon (
+ prediction_exon_id int unsigned not null auto_increment,
+ prediction_transcript_id int unsigned not null,
exon_rank smallint unsigned not null,
- exon_count smallint,
- contig_id int unsigned not null,
- contig_start int unsigned not null,
- contig_end int unsigned not null,
- contig_strand tinyint not null,
+ seq_region_id int unsigned not null,
+ seq_region_start int unsigned not null,
+ seq_region_end int unsigned not null,
+ seq_region_strand tinyint not null,
start_phase tinyint not null,
score double,
p_value double,
+
+ PRIMARY KEY( prediction_exon_id ),
+ KEY (prediction_transcript_id),
+ KEY ( seq_region_id, seq_region_start )
+);
+
+
+CREATE TABLE prediction_transcript (
+ prediction_transcript_id int unsigned not null auto_increment,
+ seq_region_id int unsigned not null,
+ seq_region_start int unsigned not null,
+ seq_region_end int unsigned not null,
+ seq_region_strand tinyint not null,
analysis_id int,
+
+ PRIMARY KEY( prediction_transcript_id ),
+ KEY ( seq_region_id, seq_region_start )
+);
+
+
+
+################################################################################
+#
+# Table structure for table 'meta'
+#
+
+CREATE TABLE meta (
+
+ meta_id INT not null auto_increment,
+ meta_key varchar( 40 ) not null,
+ meta_value varchar( 255 ) not null,
+
+ PRIMARY KEY( meta_id ),
+ KEY meta_key_index ( meta_key ),
+ KEY meta_value_index ( meta_value )
- PRIMARY KEY( prediction_transcript_id, exon_rank ),
- KEY (contig_id)
);
+# Auto add schema version to database
+
+INSERT INTO meta (meta_key, meta_value) VALUES ("schema_version", "$Revision$");
+
+################################################################################
+#
+# Table structure for table 'marker_synonym'
CREATE TABLE marker_synonym (
- marker_synonym_id int unsigned not null auto_increment,
- marker_id int unsigned not null, #foreign key marker:marker_id
- source varchar(20),
- name varchar(30),
- PRIMARY KEY (marker_synonym_id),
- KEY marker_synonym_idx (marker_synonym_id, name),
- KEY marker_idx (marker_id)
+ marker_synonym_id int unsigned not null auto_increment,
+ marker_id int unsigned not null, # foreign key marker:marker_id
+ source varchar(20),
+ name varchar(30),
+
+ PRIMARY KEY (marker_synonym_id),
+ KEY marker_synonym_idx (marker_synonym_id, name),
+ KEY marker_idx (marker_id)
);
+
+################################################################################
+#
+# Table structure for table 'marker'
+
CREATE TABLE marker (
- marker_id int unsigned not null auto_increment,
- display_marker_synonym_id int unsigned, #foreign key marker_synonym:marker_synonym_id
- left_primer varchar(100) not null,
- right_primer varchar(100) not null,
- min_primer_dist int(10) unsigned not null,
- max_primer_dist int(10) unsigned not null,
- priority int,
- type enum('est', 'microsatellite'),
-
- PRIMARY KEY (marker_id),
- KEY marker_idx (marker_id, priority)
+
+ marker_id int unsigned not null auto_increment,
+ display_marker_synonym_id int unsigned, #foreign key marker_synonym:marker_synonym_id
+ left_primer varchar(100) not null,
+ right_primer varchar(100) not null,
+ min_primer_dist int(10) unsigned not null,
+ max_primer_dist int(10) unsigned not null,
+ priority int,
+ type enum('est', 'microsatellite'),
+
+ PRIMARY KEY (marker_id),
+ KEY marker_idx (marker_id, priority)
+
);
+################################################################################
+#
+# Table structure for table 'marker_feature'
CREATE TABLE marker_feature (
- marker_feature_id int unsigned not null auto_increment,
- marker_id int unsigned not null, #foreign key marker:marker_id
- contig_id int(10) unsigned NOT NULL, #foreign key contig:contig_id
- contig_start int(10) unsigned NOT NULL,
- contig_end int(10) unsigned NOT NULL,
- analysis_id int(10) unsigned NOT NULL, #foreign key analysis:analysis_id
- map_weight int(10) unsigned,
- PRIMARY KEY (marker_feature_id),
- KEY contig_idx (contig_id )
+ marker_feature_id int unsigned not null auto_increment,
+ marker_id int unsigned not null, #foreign key marker:marker_id
+ seq_region_id int(10) unsigned NOT NULL, #foreign key contig:seq_region_id
+ seq_region_start int(10) unsigned NOT NULL,
+ seq_region_end int(10) unsigned NOT NULL,
+ analysis_id int(10) unsigned NOT NULL, #foreign key analysis:analysis_id
+ map_weight int(10) unsigned,
+
+ PRIMARY KEY (marker_feature_id),
+ KEY seq_region_idx (seq_region_id, seq_region_start )
+
);
-
+
+################################################################################
+#
+# Table structure for table 'marker_map_location'
+
CREATE TABLE marker_map_location (
- marker_id int unsigned not null, #foreign key marker:marker_id
- map_id int unsigned not null, #foreign key map:map_id
- chromosome_id int unsigned not null, #foreign key chromosome:chromosome_id
- marker_synonym_id int unsigned not null, #foreign key marker_synonym:marker_synonym_id
- position varchar(15) not null,
- lod_score double,
-
- PRIMARY KEY (marker_id, map_id),
- KEY map_idx( map_id, chromosome_id, position)
+
+ marker_id int unsigned not null, #foreign key marker:marker_id
+ map_id int unsigned not null, #foreign key map:map_id
+ chromosome_name varchar(15) not null,
+ marker_synonym_id int unsigned not null, #foreign key marker_synonym:marker_synonym_id
+ position varchar(15) not null,
+ lod_score double,
+
+ PRIMARY KEY (marker_id, map_id),
+ KEY map_idx( map_id, chromosome_name, position)
+
);
+################################################################################
+#
+# Table structure for table 'map'
+
CREATE TABLE map (
- map_id int unsigned not null auto_increment,
- map_name varchar(30) not null,
- PRIMARY KEY (map_id)
+ map_id int unsigned not null auto_increment,
+ map_name varchar(30) not null,
+
+ PRIMARY KEY (map_id)
);
-
+################################################################################
#
-# Table structure for table 'mapfrag'
+# Table structure for table 'misc_feature'
#
-CREATE TABLE mapfrag (
- mapfrag_id int(10) unsigned NOT NULL auto_increment,
- type enum('clone','superctg','assembly_contig','band','chr','matepair', 'haploblock') NOT NULL default 'clone',
- dnafrag_id int(10) unsigned NOT NULL default '0',
- seq_start int(10) unsigned NOT NULL default '0',
- seq_end int(10) unsigned NOT NULL default '0',
- orientation tinyint(4) NOT NULL default '0',
- name varchar(40) NOT NULL default '',
- PRIMARY KEY (mapfrag_id),
- KEY name_idx( name ),
- KEY m(dnafrag_id,seq_start)
+CREATE TABLE misc_feature (
+
+ misc_feature_id int(10) unsigned NOT NULL auto_increment,
+ seq_region_id int(10) unsigned NOT NULL default '0',
+ seq_region_start int(10) unsigned NOT NULL default '0',
+ seq_region_end int(10) unsigned NOT NULL default '0',
+ seq_region_strand tinyint(4) NOT NULL default '0',
+
+ PRIMARY KEY (misc_feature_id),
+ KEY seq_region_idx( seq_region_id, seq_region_start )
+
) TYPE=MyISAM;
+
+################################################################################
#
-# Table structure for table 'dnafrag'
+# Table structure for table 'misc_attrib'
#
-CREATE TABLE dnafrag (
- dnafrag_id int(10) unsigned NOT NULL auto_increment,
- name varchar(40) NOT NULL default '',
- dnafrag_type enum('RawContig','Chromosome') default NULL,
- PRIMARY KEY (dnafrag_id),
- UNIQUE KEY name(name)
+CREATE TABLE misc_attrib (
+ misc_feature_id int(10) unsigned NOT NULL default '0',
+ attrib_type_id smallint(5) unsigned NOT NULL default '0',
+ value varchar(255) NOT NULL default '',
+
+ KEY type_val_idx( attrib_type_id, value ),
+ KEY misc_feature_idx( misc_feature_id )
) TYPE=MyISAM;
+
+################################################################################
#
-# Table structure for table 'mapannotation'
+# Table structure for table 'seq_region_attrib'
#
-CREATE TABLE mapannotation (
- mapannotation_id int(10) unsigned NOT NULL auto_increment,
- mapfrag_id int(10) unsigned NOT NULL default '0',
- mapannotationtype_id smallint(5) unsigned NOT NULL default '0',
- value varchar(240) NOT NULL default '',
- PRIMARY KEY (mapannotation_id),
- KEY mapfrag_id(mapfrag_id,mapannotationtype_id),
- KEY mapannotationtype_id(mapannotationtype_id,mapfrag_id),
- KEY value(value,mapannotationtype_id),
- KEY mapannotationtype_id_2(mapannotationtype_id,value)
+CREATE TABLE seq_region_attrib (
+ seq_region_id int(10) unsigned NOT NULL default '0',
+ attrib_type_id smallint(5) unsigned NOT NULL default '0',
+ value varchar(255) NOT NULL default '',
+
+ KEY type_val_idx( attrib_type_id, value),
+ KEY seq_region_idx (seq_region_id)
+
) TYPE=MyISAM;
+
+################################################################################
#
-# Table structure for table 'mapannotationtype'
+# Table structure for table 'attrib_type'
#
-CREATE TABLE mapannotationtype (
- mapannotationtype_id smallint(5) unsigned NOT NULL auto_increment,
- code varchar(15) NOT NULL default '',
- name varchar(255) NOT NULL default '',
- description text NOT NULL,
- PRIMARY KEY (mapannotationtype_id),
+CREATE TABLE attrib_type (
+
+ attrib_type_id smallint(5) unsigned NOT NULL auto_increment,
+ code varchar(15) NOT NULL default '',
+ name varchar(255) NOT NULL default '',
+ description text NOT NULL,
+
+ PRIMARY KEY ( attrib_type_id),
UNIQUE KEY c(code)
+
) TYPE=MyISAM;
+################################################################################
#
-# Table structure for table 'mapset'
+# Table structure for table 'misc_set'
#
-CREATE TABLE mapset (
- mapset_id smallint(5) unsigned NOT NULL auto_increment,
- code varchar(15) NOT NULL default '',
- name varchar(255) NOT NULL default '',
- description text NOT NULL,
- max_length int unsigned not null,
- PRIMARY KEY (mapset_id),
+CREATE TABLE misc_set (
+
+ misc_set_id smallint(5) unsigned NOT NULL auto_increment,
+ code varchar(15) NOT NULL default '',
+ name varchar(255) NOT NULL default '',
+ description text NOT NULL,
+ max_length int unsigned not null,
+
+ PRIMARY KEY (misc_set_id),
UNIQUE KEY c(code)
+
) TYPE=MyISAM;
+################################################################################
#
-# Table structure for table 'mapfrag_mapset'
+# Table structure for table 'misc_feature_misc_set'
#
-CREATE TABLE mapfrag_mapset (
- mapfrag_id int(10) unsigned NOT NULL default '0',
- mapset_id smallint(5) unsigned NOT NULL default '0',
- PRIMARY KEY (mapset_id,mapfrag_id)
+CREATE TABLE misc_feature_misc_set (
+
+ misc_feature_id int(10) unsigned NOT NULL default '0',
+ misc_set_id smallint(5) unsigned NOT NULL default '0',
+
+ PRIMARY KEY ( misc_feature_id, misc_set_id ),
+ KEY reverse_idx( misc_set_id, misc_feature_id )
+
) TYPE=MyISAM;
+################################################################################
+#
+# Tables for QTLs
+#
+################################################################################
+################################################################################
#
-# Tables for qtls
+# Table structure for table 'qtl'
#
CREATE TABLE qtl (
- qtl_id int unsigned auto_increment not null,
- trait varchar(255) not null,
- lod_score float,
- flank_marker_id_1 int,
- flank_marker_id_2 int,
- peak_marker_id int,
- primary key ( qtl_id ),
- key trait_idx( trait )
+
+ qtl_id int unsigned auto_increment not null,
+ trait varchar(255) not null,
+ lod_score float,
+ flank_marker_id_1 int,
+ flank_marker_id_2 int,
+ peak_marker_id int,
+
+ PRIMARY KEY ( qtl_id ),
+ KEY trait_idx( trait )
+
);
+################################################################################
+#
+# Table structure for table 'qtl_synonym'
+#
+
CREATE TABLE qtl_synonym (
- qtl_synonym_id int unsigned auto_increment not null,
- qtl_id int unsigned not null,
- source_database enum("rat genome database", "ratmap") not null,
- source_primary_id varchar(255) not null,
- primary key (qtl_synonym_id),
- key qtl_idx(qtl_id)
+
+ qtl_synonym_id int unsigned auto_increment not null,
+ qtl_id int unsigned not null,
+ source_database enum("rat genome database", "ratmap") not null,
+ source_primary_id varchar(255) not null,
+
+ PRIMARY KEY (qtl_synonym_id),
+ KEY qtl_idx(qtl_id)
+
);
+################################################################################
+#
+# Table structure for table 'qtl_feature'
+#
+
CREATE TABLE qtl_feature (
- chromosome_id int not null,
- start int not null,
- end int not null,
- qtl_id int not null,
- analysis_id int not null,
- key( qtl_id ),
- key loc_idx( chromosome_id, start )
+
+ seq_region_id int not null,
+ seq_region_start int not null,
+ seq_region_end int not null,
+ qtl_id int not null,
+ analysis_id int not null,
+
+ KEY( qtl_id ),
+ KEY loc_idx( seq_region_id, seq_region_start )
);
+################################################################################
#
-# tables for stable id mapping tracking
+# Tables for stable ID mapping tracking
+#
+################################################################################
+
+################################################################################
+#
+# Table structure for table 'mapping_session'
#
CREATE TABLE mapping_session (
- mapping_session_id int(11) NOT NULL auto_increment,
- old_db_name varchar(80) NOT NULL default '',
- new_db_name varchar(80) NOT NULL default '',
- created timestamp(14) NOT NULL,
+
+ mapping_session_id int(11) NOT NULL auto_increment,
+ old_db_name varchar(80) NOT NULL default '',
+ new_db_name varchar(80) NOT NULL default '',
+ created timestamp(14) NOT NULL,
+
PRIMARY KEY (mapping_session_id)
+
) TYPE=MyISAM;
+################################################################################
+#
+# Table structure for table 'stable_id_event'
+#
+
CREATE TABLE stable_id_event (
- old_stable_id varchar(40),
- old_version smallint,
- new_stable_id varchar(40),
- new_version smallint,
- mapping_session_id int(11) NOT NULL default '0',
- type ENUM('gene', 'transcript', 'translation') NOT NULL,
+
+ old_stable_id varchar(128),
+ old_version smallint,
+ new_stable_id varchar(128),
+ new_version smallint,
+ mapping_session_id int(11) NOT NULL default '0',
+ type ENUM('gene', 'transcript', 'translation') NOT NULL,
+
UNIQUE KEY tpl_idx (old_stable_id,new_stable_id,mapping_session_id),
KEY new_idx (new_stable_id)
+
) TYPE=MyISAM;
+################################################################################
+#
+# Table structure for table 'gene_archive'
+#
+
CREATE TABLE gene_archive (
- gene_stable_id VARCHAR(40) NOT NULL,
- gene_version smallint NOT NULL,
- transcript_stable_id VARCHAR(40) NOT NULL,
- transcript_version smallint NOT NULL,
- translation_stable_id VARCHAR(40) NOT NULL,
- translation_version smallint NOT NULL,
- mapping_session_id int NOT NULL,
+
+ gene_stable_id VARCHAR(128) NOT NULL,
+ gene_version smallint NOT NULL,
+ transcript_stable_id VARCHAR(128) NOT NULL,
+ transcript_version smallint NOT NULL,
+ translation_stable_id VARCHAR(128) NOT NULL,
+ translation_version smallint NOT NULL,
+ mapping_session_id int NOT NULL,
+
KEY gene_idx( gene_stable_id, gene_version ),
KEY transcript_idx( transcript_stable_id, transcript_version )
+
) TYPE=MyISAM;
+################################################################################
+#
+# Table structure for table 'peptide_archive'
+#
+
CREATE TABLE peptide_archive (
- translation_stable_id VARCHAR(40) NOT NULL,
- translation_version smallint NOT NULL,
- peptide_seq mediumtext NOT NULL,
+
+ translation_stable_id VARCHAR(128) NOT NULL,
+ translation_version smallint NOT NULL,
+ peptide_seq mediumtext NOT NULL,
PRIMARY KEY( translation_stable_id, translation_version )
+
+) TYPE=MyISAM;
+
+################################################################################
+#
+# Table structure for table 'seq_region'
+#
+
+CREATE TABLE seq_region (
+
+ seq_region_id INT(10) UNSIGNED NOT NULL AUTO_INCREMENT,
+ name VARCHAR(40) NOT NULL,
+ coord_system_id INT(10) NOT NULL,
+ length INT(10) NOT NULL,
+
+ UNIQUE(coord_system_id, name),
+ PRIMARY KEY (seq_region_id)
+
+) TYPE=MyISAM;
+
+
+################################################################################
+#
+# Table structure for table 'assembly_exception'
+#
+
+CREATE TABLE assembly_exception (
+
+ assembly_exception_id INT(10) UNSIGNED NOT NULL AUTO_INCREMENT,
+ seq_region_id INT NOT NULL,
+ seq_region_start INT NOT NULL,
+ seq_region_end INT NOT NULL,
+ exc_type ENUM('HAP', 'PAR') NOT NULL,
+ exc_seq_region_id INT NOT NULL,
+ exc_seq_region_start INT NOT NULL,
+ exc_seq_region_end INT NOT NULL,
+ ori INT NOT NULL,
+
+ PRIMARY KEY (assembly_exception_id),
+
+ KEY sr_idx (seq_region_id, seq_region_start),
+ KEY ex_idx (exc_seq_region_id, exc_seq_region_start)
+
+) TYPE=MyISAM;
+
+################################################################################
+#
+# Table structure for table 'coord_system'
+#
+
+CREATE TABLE coord_system (
+
+ coord_system_id INT NOT NULL auto_increment,
+ name VARCHAR(40) NOT NULL,
+ version VARCHAR(40),
+ attrib SET ('top_level', 'default_version', 'sequence_level'),
+
+ UNIQUE(name, version),
+ PRIMARY KEY (coord_system_id)
+
+) TYPE=MyISAM;
+
+################################################################################
+#
+# Table structure for table 'meta_coord'
+#
+
+CREATE TABLE meta_coord (
+
+ table_name VARCHAR(40) NOT NULL,
+ coord_system_id INT NOT NULL,
+
+ UNIQUE(table_name, coord_system_id)
+
+) TYPE=MyISAM;
+
+
+
+CREATE TABLE density_feature (
+ density_feature_id INT NOT NULL auto_increment,
+ density_type_id INT NOT NULL, #FK refs density_type
+ seq_region_id INT NOT NULL, #FK refs seq_region
+ seq_region_start INT NOT NULL,
+ seq_region_end INT NOT NULL,
+ density_value FLOAT NOT NULL,
+
+ PRIMARY KEY(density_feature_id),
+ KEY seq_region_idx (density_type_id, seq_region_id, seq_region_start)
+
+) TYPE=MyISAM;
+
+
+
+CREATE TABLE density_type (
+ density_type_id INT NOT NULL auto_increment,
+ analysis_id INT NOT NULL, #FK refs analysis
+ block_size INT NOT NULL,
+ value_type ENUM('sum','ratio') NOT NULL,
+
+ PRIMARY KEY(density_type_id),
+ KEY analysis_idx (analysis_id, block_size)
+
) TYPE=MyISAM;
--
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