add build_gb script

emeraldbgc/.ipynb_checkpoints/pkg_info-checkpoint.json

+{
+    "name": "emeraldbgc",
+    "version": "0.2.3",
+    "description": "SMBGC detection tool",
+    "author": "Santiago Sanchez Fragoso",
+    "author_email": "fragoso@ebi.ac.uk",
+    "url": "https://gitlab.ebi.ac.uk/fragoso/emerald",
+    "license": "Apache License, Version 2.0"
+}

emeraldbgc/_cli.py

@@ -49,7 +49,7 @@ def main(args=None):
         dest="ip_file",
         default=None,
         type=str,
-        help="Optional, preprocessed InterProScan GFF3 output file. Requires a GBK file as SEQUENCE_FILE. The GBK must have CDS as features, and \"protein_id\" matching the ids in the InterProScan file",
+        help="Optional, preprocessed InterProScan GFF3 output file. Requires a GBK file as SEQUENCE_FILE. The GBK must have CDS as features, and \"protein_id\" matching the ids in the InterProScan file. The GBK file can be build with emerald_build_gb tool",
         metavar="FILE",
     )
     parser.add_argument(

emeraldbgc/build_gb.py

+#!/usr/bin/env python3
+
+# Copyright 2021 EMBL - European Bioinformatics Institute
+#
+# Licensed under the Apache License, Version 2.0 (the "License");
+# you may not use this file except in compliance with the License.
+# You may obtain a copy of the License at
+# http://www.apache.org/licenses/LICENSE-2.0
+#
+# Unless required by applicable law or agreed to in writing, software
+# distributed under the License is distributed on an "AS IS" BASIS,
+# WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
+# See the License for the specific language governing permissions and
+# limitations under the License.
+
+import argparse
+import sys
+import glob
+import os
+from Bio import SeqIO
+
+def main(args=None):
+
+    parser = argparse.ArgumentParser(description="build_gb. Tool to build genbank format files ")
+    parser.add_argument(
+        "-n",
+        dest="nuc_f",
+        default=None,
+        type=str,
+        help="FASTA file with nucleotide sequence(s)",
+        metavar="FILE",
+        required=True,
+    )
+    parser.add_argument(
+        "-a",
+        dest="pro_f",
+        default=None,
+        type=str,
+        help="prodigal output FASTA file with aminoacids sequences",
+        metavar="FILE",
+        required=True,
+    )
+    parser.add_argument(
+        "-o",
+        dest="out",
+        default=None,
+        type=str,
+        help="output genebank format file",
+        metavar="FILE",
+        required=True,
+    )
+    
+    args = parser.parse_args(args)
+
+    fna = {rec.id:rec.seq for rec in SeqIO.parse(open(args.nuc_f),'fasta')}
+    faa = list(SeqIO.parse(open(args.pro_f),'fasta'))
+
+    feats = {}
+
+    for ff in faa:
+        spl = ff.description.split()
+        s,e,st = int(spl[2])-1,int(spl[4]),int(spl[6])
+        
+        from Bio import SeqFeature
+        start_pos = SeqFeature.ExactPosition(s)
+        end_pos = SeqFeature.ExactPosition(e)
+
+        from Bio.SeqFeature import FeatureLocation
+        feature_location = FeatureLocation(start_pos,end_pos)
+
+        feature_type = "CDS"
+
+        from Bio.SeqFeature import SeqFeature
+        qual = {}
+        qual['translation'] = str(ff.seq).replace("*","")
+        qual['protein_id'] = str(ff.id)
+        feature = SeqFeature(feature_location,type=feature_type,qualifiers=qual)
+
+        cont = "_".join(ff.id.split('_')[:-1])
+        feats.setdefault(cont,[]).append(feature)
+
+    recs = []
+    for cont,v in feats.items():
+
+        sequence = fna.get(cont)
+
+        from Bio.SeqRecord import SeqRecord
+        sequence_record = SeqRecord(sequence)    
+        sequence_record.id = cont    
+        sequence_record.name = cont    
+        sequence_record.description = cont    
+        sequence_record.annotations={"molecule_type": "DNA"}
+
+        sequence_record.features = v
+
+        recs.append(sequence_record)
+
+
+
+    with open(args.out, 'w') as h:
+        SeqIO.write(recs, h, 'genbank')
+    print(f"Done!.. outfile :{args.out}")
+
+if __name__ == "__main__":
+    main(sys.argv[1:])
\ No newline at end of file

emeraldbgc/get_ips_slim.sh

+#!/usr/bin/env bash
+
+if [[ "$OSTYPE" == "linux-gnu"* ]]; then
+  wget ftp://ftp.ebi.ac.uk/pub/software/unix/iprscan/5/5.52-86.0/interproscan-5.52-86.0-64-bit.tar.gz
+  wget ftp://ftp.ebi.ac.uk/pub/software/unix/iprscan/5/5.52-86.0/interproscan-5.52-86.0-64-bit.tar.gz.md5
+  md5sum -c interproscan-5.52-86.0-64-bit.tar.gz.md5
+  PY=$CONDA_PREFIX/bin/python
+  PKG_FILE=$($PY -c "import ${PKG_NAME};print(${PKG_NAME}.__file__)")
+  PKG_DIR=$(dirname $PKG_FILE)
+  echo $PKG_DIR >> ~/env.test.txt
+  tar --exclude-from=${PKG_DIR}/exclude.txt -pxvzf interproscan-5.52-86.0-*-bit.tar.gz -C ${PKG_DIR}
+  rm interproscan-5.52-86.0-*-bit.tar.gz
+  ln -s ${PKG_DIR}/interproscan-5.52-86.0/interproscan.sh ${CONDA_PREFIX}/bin
+
+  cd ${PKG_DIR}/interproscan-5.52-86.0/
+  $PY initial_setup.py 
+
+else
+  echo "Non Linux OS; not installing InterProScan"
+fi

setup.py

@@ -36,9 +36,8 @@ setup(
     entry_points = {
             'console_scripts': [
                                 'emeraldbgc = emeraldbgc._cli:main',
-#                                'emerald_download_data = emeraldbgc.download_data:main',
+                                'emerald_build_gb = emeraldbgc.build_gb:main',
                                 ]
     },
     packages = find_packages(exclude=('tests', 'docs')),
-#    exclude_package_data={"emeraldbgc": ["interproscan"]},
 )

test/files/.ipynb_checkpoints/BGC0001472-checkpoint.fna

+>BGC0001472
+GCCCCGGGGGCCGTCGCTCCGGGGGTCGGTCCTGCCCGGTGGCGCAGGACCACGGGGGCCGGGGCCCGGGGGTGGACGGCATTTGTTTTGACCCAGCTCCGTGAGGTAGGTACGCTCAAGCCTTGTGCCTGGGGTGTGCCTGGGCTCGGGTGCGTGTCCTCAACCGCATGGCGAGTCCGTAAGTGGCCACCGCAATCTGTGTTCCGTCTGCCTTCCAGCAGGGGCGTGCAGTATTCGACACACCCGACCGCGTGGGTCGGTGACTGTTCCAGGTTAGTTTCACCGAACGGCACACAGAAACCGGAGAAGTAGTGCCTACGATCCAGCAGCTGGTCCGGAAGGGCCGGCAGGACAAGGTCGAGAAGAACAAGACGCCCGCGCTCGAGGGTTCGCCCCAGCGTCGTGGTGTCTGCACGCGTGTGTTCACGACCACCCCGAAGAAGCCGAACTCGGCGCTCCGTAAGGTCGCGCGTGTGCGTCTGACCTCCGGTATCGAGGTCACGGCCTACATCCCGGGTGAGGGGCACAACCTGCAGGAGCACTCCATCGTGCTCGTGCGTGGTGGCCGTGTGAAGGACCTGCCGGGTGTTCGTTACAAGATCATCCGCGGTTCGCTCGACACCCAGGGTGTCAAGAACCGCAAGCAGGCCCGCAGCCGCTACGGCGCCAAGAAGGAGAAGTAAGAATGCCTCGTAAGGGCCCCGCCCCGAAGCGCCCGGTCATCATCGACCCGGTCTACAGCTCTCCTCTTGTCACCTCGCTGATCAACAAGATCCTGCTCGACGGCAAGCGTTCCACCGCCGAGCGGATCGTGTACGGCGCCATGGAAGGCCTCCGCGAGAAGACCGGCGCTGACCCGGTCATCACGCTGAAGCGCGCGCTTGAGAACGTCAAGCCCTCGCTCGAGGTCAAGTCCCGCCGTGTCGGTGGCGCCACCTACCAGGTGCCGATCGAGGTCAAGCCCGGTCGCGCCGCCACCCTCGCTCTGCGCTGGGTCGTGGGTTACTCCCGCGCCCGTCGCGAGAAGACCATGACCGAGCGCCTCATGAACGAGCTGCTCGACGCCTCCAACGGTCTTGGCGCTGCCGTCAAGAAGCGCGAGGACACCCACAAGATGGCCGAGTCGAACAAGGCCTTCGCGCACTACCGCTGGTAGTCGCTCACCCCATCGAGACCGAGAGAAGATTGAGCCTTATGGCCACCACTTCGCTTGACCTGGCCAAGGTCCGCAACATCGGGATCATGGCCCACATCGACGCGGGCAAGACGACCACCACCGAGCGGATCCTCTTCTACACCGGCGTTTCGTACAAGATCGGTGAAGTCCACGACGGCGCAGCCACGATGGACTGGATGGAGCAGGAGCAGGAGCGCGGCATCACGATCACGTCCGCCGCGACGACCTGTCACTGGCCGCTCAATGATGTTGACCACACCATCAACATCATCGACACCCCGGGTCACGTCGACTTCACCGTCGAGGTGGAGCGTTCGCTCCGCGTCCTCGACGGTGCCGTCACCGTGTTCGACGGTGTGGCCGGCGTCGAGCCCCAGTCCGAGACCGTCTGGCGTCAGGCGGACCGCTACGGCGTGCCGCGTATCTGCTTCGTCAACAAGCTCGACCGCACGGGCGCCGACTTCCTCCGTTGCGTCGACATGATCGTCCAGCGCCTCGGCGCTGTCCCGATCGTCATGCAGCTCCCCATCGGTGCGGAGGCTGACTTCCGCGGCGTCGTCGACCTCGTGTCGATGAAGGCCTTCGTTTACCCCGAAGAGGCCGTCAAGGGCGAGATGTACGACACCGTCGAGATCCCGGACAACCTCAAGGAGGCCGCCGAGGAATGGCGCGGCAAGCTCCTCGAGGCCGTCTCGGAGAACGACGACCAGATGATGGAGCTGTACCTCGAGGGCGAAGAGCCCACCGAGGAGCAGCTGCACGAGGCGATCCGTCGGATCACCCTCGCGTCGAAGGGCTCGGCCGACTCCGTCACCGTGACCCCCGTCTTCTGTGGCACGGCGTTCAAGAACAAGGGCGTCCAGCCCCTGCTCGACGCCGTCGTCCGCTACCTGCCTTCCCCCCTGGACGTCGAGGCCATCGAGGGCCACGACGTCAAGGACCCGGAGAAGGTCGTCCAGCGGAAGCCCTCGGACGACGAGCCGTTCTCCGGCCTGGCGTTCAAGATCGCGAGCGACCCGCACCTCGGCAAGCTCACCTTCGTCCGGATCTACTCCGGTCGCCTCGAGGCCGGCACCGCGGTGCTGAACTCGGTCAAGGGCAAGAAGGAGCGCATCGGCAAGATCTACCGCATGCACGCGAACAAGCGTGAGGAGATCCCGTCGGTGGGCGCCGGTGACATCGTCGCCGTCATGGGCCTGAAGCAGACCACCACCGGTGAGACGCTGTGTGACGACAAGAACCCGGTGATCCTGGAGTCCATGGACTTCCCGGCGCCGGTCATCCAGGTCGCCATCGAGCCCAAGTCCAAGGGTGACCAGGAGAAGCTGGGTGTCGCCATCCAGCGCCTCTCGGAGGAGGACCCCTCCTTCCAGGTGCACTCCGACGAGGAGACCGGCCAGACCATCATCGGTGGTATGGGCGAGCTTCACCTCGAGGTGCTCGTCGACCGCATGAAGCGCGAGTTCCGCGTCGAGGCGAACGTCGGCAAGCCGCAGGTCGCGTACCGTGAGACGATCCGCAAGGCCGTCGAGCGTATCGACTACACGCACAAGAAGCAGACTGGTGGTACCGGCCAGTTCGCGAAGGTGCAGATCGCCATCGAGCCCATCGAGGGTGGCGACGCGTCCTACGAGTTCGTCAACAAGGTCACCGGTGGCCGCATCCCCCGTGAGTACATTCCCTCGGTGGACGCGGGTGCCCAGGAAGCCATGCAGTTCGGCATCCTGGCCGGCTACGAGATGGTGGGCGTCCGCGTCACCCTTCTCGACGGTGGTTACCACGAGGTCGACTCCTCGGAGCTCGCCTTCAAGATCGCTGGTTCGCAGGCGTTCAAGGAGGGTGCCCGCAAGGCGTCCCCCGTGCTCCTCGAGCCGATGATGGCCGTCGAGGTCACCACACCCGAGGACTACATGGGTGAAGTGGTCGGCGACATCAACTCCCGCCGTGGCCAGATCCAGGCCATGGAGGAGCGCCACGGCGCTCGCGTCGTGAAGGGCCTCGTGCCCCTCTCGGAGATGTTCGGCTACGTCGGAGACCTCCGCAGCAAGACCTCGGGTCGCGCAAGCTACTCGATGCAGTTCGACTCCTACGCCGAGGTTCCGCGGAACGTCGCCGAGGAGATCATCGCGAAGGCCAAGGGCGAGTAACTCTTCCGAGCTCACGCTTTAGGCTTGTCACCGGAGCCCGGTCGGGCATGCGTCGCAGTGCGGCGGATGCCCCCGGCACCGGCATTCCAGCAAAGATCACCTGGCGCCGATGAAGCAAGGCGTACAGAACCACTCAGGAGGACCCCAGTGGCGAAGGCAAAGTTCGAGCGGACTAAGCCGCACGTCAACATCGGCACCATCGGTCACATCGACCACGGTAAGACGACCCTCACGGCCGCCATTACCAAGGTGCTGCACGACGCGTACCCGGACCTGAACGAGGCCTCGGCCTTCGACCAGATCGACAAGGCTCCTGAGGAGCGTCAGCGCGGTATCACGATCTCGATCGCGCACGTCGAGTACCAGACGGAGTCGCGTCACTACGCGCACGTCGACTGCCCGGGTCACGCTGACTACATCAAGAACATGATCACGGGTGCGGCGCAGATGGACGGCGCCATCCTCGTGGTCGCGGCCACCGACGGCCCGATGCCGCAGACCAAGGAGCACGTGCTCCTGGCCCGCCAGGTAGGCGTGCCGTACATCGTCGTCGCGCTGAACAAGGCCGACATGGTGGACGACGAGGAGATCCTGGAGCTCGTCGAGCTCGAGGTCCGTGAGCTCCTCTCCGAGTACGAGTTCCCGGGCGACGACCTTCCGGTCGTCAAGGTCTCGGCGCTCAAGGCCCTCGAGGGCGACGCCGAGTGGGGCCAGACCGTTCTCGACCTGATGAAGGCCGTCGACGAGTCCATCCCGCAGCCCGAGCGTGACGTCGAGAAGCCGTTCCTCATGCCCATCGAGGACGTCTTCACGATCACCGGTCGCGGTACGGTCGTCACCGGCCGCATCGAGCGTGGTGTCCTGAAGGTCAACGAGACCGTCGACATCGTCGGTATCAAGACCGAGAAGACCACCACCACGGTCACCGGCATCGAGATGTTCCGCAAGCTGCTCGACGAGGGCCAGGCCGGTGAGAACGTCGGTCTGCTGCTTCGTGGCATCAAGCGCGAGGACGTCGAGCGCGGCCAGGTCATCATCAAGCCGGGTTCGGTCACGCCGCACACCGAGTTCCAGGCCCAGGCCTACATCCTGTCGAAGGACGAGGGTGGCCGTCACACCCCCTTCTTCAACAACTACCGCCCGCAGTTCTACTTCCGTACCACGGACGTGACGGGCGTTGTGACCCTTCCCGAGGGCACCGAGATGGTCATGCCGGGTGACAACACCCTCATGGACGTCGCGCTGATCCAGCCGGTCGCCATGGAAGAGGGCCTGAAGTTCGCCATCCGTGAGGGTGGTCGTACGGTGGGCGCCGGCCAGGTCACCAAGATCACCAAGTAATTCCGATTACTTGTGGGTCGGGGTAACCCGGTTGCTCTGAACTGAGCGCACAGCACCAAGCAGGGCCCGCACGGCATCACGCCGTGCGGGCCCTGTGCTGTCTCCGCGGGGCTCGCGGCGGACAGGACGACGGGCGCGGCCCGCACACCCTGAGGGTGTGCGGGCCGTCGTGACGCGGGCGCCGTTCGCGTCGGGAGGTGCGGTCAGCTCTCCGGTGGCCGGGCGTATGTGGTGGCCAGCAGGGAATGGCGGGTCGCCCCGTCGCTGTGGACCAGATGGTGCACGGACGACGGGACGAAGCCGCCGAACAGTGTGCCGACGAGCCCCAGCGCCTGGCACCTGAGATTGAGGTCGTAGGTGGCCATCGAGGCCCGCAGCGCACTGATCCGGTAGCCGTGGGAACCCGCCCAGGTGTCCGCCCGGTCCAGACTCGCGTAGAGCGCGACGATCCCCGCTCCGGTGGAGAACTCGCGCTGGACGCCGAGGTTCTCGGCCGGTCCGATCTCGTCGAGCCCGGCCAGGTAGCACGTCTCCTCGGCGGTCACCCGGTACAGCCCTGGTTCGGCGCCCTCGCTGCGGAACGCGAAGACGAAGCCCTCCAGCGCGCCCGCCGAGGCGTCGAGCCCCCAGTCGTCACGGTCCCGGCGCAGCACGTCACGCAGCAGCGACAGGATCACGTCCGTGCGTACCGGCAGTGGCGCGTAGTGCAGCGAGGAGCGGCGGCGTTCGAGCGTGCCCCGCAGGTCGTGGACCGGCCGCAGGTCCTCGGCAGACGGTTGCGGTTCGACGGGCACGGAGTCCGCGAACGGCACCGGCCGGCGTTCGGCCGCCAGGCCGTCCGACGTGGGCGTCCGGGAGGTGAAGCCCTCCAGCACGGCCGTCATGGATGTCACGTCGTTCCTCATGCGGTACCTCCCAGGGACGCGAAGGCCGCCACCGGCTCGTCGGCAGGCGATGTTCCGAGCAGCCGGGCCAACGCGTCGTCGTCCCAGTCCGACCTGGGTGTGAAGCCCAGGCCCAGGTGCTGTGCGAGCTCCCGCAGACTGGCCAGGTTGCAGCCCGCGTCCAGAAAGACCAGCCTGAAGCCGAACGTGCCGTACTTGGTCATGACCTTCTTGAGATCGCCGGTGATGATGATGTCGCACGGCGAGTCACCCGGAGGTACCTCGCCGGAGACGGTCACGAGTGTGTGGCTGCCCTGGGCGTACGCGTAGACCCCAGGAGGCATGATCCGGTCGTCGCGCACCACCGCGTAGGCCGTCGTGCTGCCGATGTTTCCCGACGCCGCGGTCCATCGTTTGACGCGCTCCGGAGTGGTCTCGTCCTCCTTCACCCCGAAGGCGACCTTCAGCAGCAGTCCCAGGGAGCTCAGGGTGAGCGGGGTGTCGCCGTGGGACGGGTCATGCCGTTCCGACCCGGCGAGGACGGAGATGTCCAGGGCGGGGAGCGGTGTGTGCGGGCGGCTCGGCCAGTCCTTGAACTGCGACTGCAGGCGCAGGTTGGACGCGTAGTAGTGCGCCTGGTGGTCCTTCGGAGCGAGGAACGCGCGTGGAGGCATCGCGACCGAGGCCTCGTAGACGGCACCGGCGGGTGCCTGGGGTGCGACCGGCCCCCGCGCGTAGGAGCAGCGAGGGCACCCCGGGCGGACGGCAACCGGCCGGTAGACCGTGCTCAGGGTCGCGGTGTCGATGACGGTGAAGTCGCCGGGGAGGTGCGAGATCGTGGCCCGTGCGAGCAACGCGGTGACGTGGTGGGAGGCCAGGCCGACGACCAGGTCGTGCAGGTATTCCGGCGGCTCGCCGGAGAGGTCCGCCTCACCGTGACGGCCGCAGTCCAGGCACGGTGTGATCGAGAGGTCGGCGTACGGGCCGATCGTGATCGTCCGCGCGTCGGCGCGCACCCGCAGCAGCGGACGCCCGTCCTGCCGGCACCGTTCCTCGGTGGCGGCGAGCAGGGGTGCCGACGCGGGCGTCTCGAAGAACACGGTGAGCTCGTCACCGGGGCCCGGCGGCCCGGCCGGTTCCGTGACCACCGGGCACACCTCCCGCAGCGACCGGCGGGCCCCGGCGACCAGGGCGGCGTCCCCCTCGAGGCGCACCGAGCGCGACACCAGCCGCGCCGCGGCATCCGCCCAGGACGGGTTCGATCCGGTGGAGTTGCCCAGCCGGGAGAGGAAGACGGCCCATTCGGGCGTGACGTCCGGTTCCTCGCCGGACATGGCCTCCTCGACCGCCCCGGCCGTCGACAGCAAGGCCAGGCACTTGTAGACCGTGGCCTCGTCGAATCCGGTCTTGAGCGCCAGTTCAGTGTGGTCGCGGGTTCCGTCGCACGCCTCGGTCAGGGGCACCAGCCCTTCTCGTGCGAACGCTCCGGAGAACACCTGTGCCCGGTCGGCCCCGTCGAGCACGACGGATTCCCCTGCCCTGCGCAGCCTCACCCCGCGGCGCAGGACGGGCCGGGCAGGGACGGTCAACTGCATGTCCGTACGTGCGGCCCGCCCGATCTGCTCGGCCTTCATCTCGGCAACCATGATCGATCTCCCTCTTACCGCAGCAGTTCCGGAACAGGCATGGCGCTGTCCGCGCGCGCCTGCGAGAACCGGCCCCCGCACCGGGGGCACCGGTTCACCGAGACCGTCACCGCGGACGAGACGGCTCCGGAGACGAGATTGAACGTACGTACGGTGCCCCCGATCCCCGTCACACCCGTGGCGATCTCGGTCAGGGCGAGGTCCAGGAGGCCGGAGGCGACGGACAGGTGCTGCCGGCCGAACCCCTCGGGCAGGCCGGACAGGGCGGCGTCCATGTCGTACGGGCGGGCGGTGCCCGCGTGTTGGGCGGCGCGCTTCCTGTAGCACGCGTAGCAGGCCGTCCGGCCCGGAACCACGACGGGTCCGCAGAGGACCTTGGTCGGCAGCAGCTGGAGCCCGACGGACGGTGTGCTCCGTTCCGCGCAGATCCGGTCCGTCTCGTCGCGGAGCCCGGGGTCGCCGCCGGCATGCACGGGGACGACGACATCCGCGTGCGGTGCCGTGTCGTTCAGGAAGTCCTCGAAGGACACCTGTAGCTCCGGGGGGACACCACGGTGCTCCGTGAGCCGCCGGCTGAAGGTGTCACCCACGAGGTACAGGCACTGCGGACGCGGATCGTGCGACATGCTGCTCTGCTCCTTCTTCTCGCTCATGCGAACGGCTGCTGCACGGGGTTGACCGCGTCCTCGGCATGGCTCGTGTGCCCCATCGCGCGCGGCGCGTCATAGAGGCGCGGGGTGCCCAGGTACCGCTCGCCGTGTACGAACGACACGGGCATGGCCTCGGGCACGAGCACCTTGACCGCCCGCATGCCCACCTGGCGCGCCTCGTCCGTGGTGATGTCCGTGACGAGCACCTCGGCACCCCGTGCCGCGAGCCGGGCGACGACGGTGTCCAGCGGGTCCGCTCCCGCCGGAAGCCCCGGCATGCCTTCGAGGCCGTACGCCGGCCGTTCACCGTCCAGCAGGAAGCCGAACACGTCCCGCCGGTCGCGCGTCGCGTTGTGGACCGCGCCGCCGACGACGCTCACCTTGGCCGGATCGGGCTCGCGCCCGGCGTACGCCGAGAGGTATCCGCGCAACGCCACCCGCAGGGAGGCCAGTTCGCGGTAGATCTTGCCCAGCGCCTGTTCCGGATGGACGTCGCAGGTGGCGGCGACGATCTGGGCGAGCGCGGGGTCGGCGTCGGAGAGCTGCACGGCGTAGATCACCGGGACGCCGAAGTCCGTCGTCGCGTCGAACAGCCTCACCCGCAGGTCCGTGGAGGTGCCGACGCGGTGCAGTTCCCGGACGCCGGCGTCGAGCCGCGCCGGATCCACGACCAGCTCGGGCAGCCGCAGCTGCTGGAGCCAGACCAGCGCGATGGCGTCCCGCTCGACGACTTCCAGCAACCCGCCGAGCACGGCGCTGCGTACGTCGGAGTGGACGGCGGCACCCGTGGTGATCCCGCGGATGAACTCTTCGGACTTCGACTGGTAGGGCATGTGCAGATACACGGAGATCGCCGGCACGAGCACCGGTATCCGCCGGGTGAGCGACCATGCGCGCACCCATCGGATCGGGACCGAGGGGTCGTACGCGGACAGGCTGCAGTCGTCGCGGGCCAGCTCGGTGGGCGAGCAGCTGGGCCACCGGGACGGCGACACGAACTCCTCTGTGAGGTCGTTCTCGGCGGCGACCACCATCTCGTCGTCGTCCCAGGCACACGTGGAGTACCGTTCGAGCGCTTCCGCGATGGAGACCAGCTTGGCGCGCTCGGGCGTGAGCCCGGTGCCCGCGCCGTCGGAATTTCCGGTGTCCTCGTCGTGGGCCCACGTCCGCAGGTTGGGAAGAGCACGGGACGGGACACCGAGATAGGCCAGCTGGACGGCGAAGGGCGGTTCGCCCTCGCGCACCGGCAGCGGCGCGGTGCGTGACACCAGTCCGTAGGGCGAGACGAGCTCCTCGAGACCGCGCAGCTCGGCCGAGATGTGCTCCTGAGGCCTGTTCAGATGCATGTGGTTCTTCTTCCCGCGTCCGGATGTTCCCCGTGTCCTTGCCGCGCTACTCGGCGGTCTTCGTGGATCCCGACGCCATGCTCCTGGCCAGGCCGTCGAGCTCGTCCTGGAGCTCCTTCATGGACTTTCCGCAGACGTCTTCGTTCGCACGTGCGACGACGTAGCAGAAGACGTACCTCTCCGCCAGGCTGAAACCGAGGGTGTACAGGCAGCTGTACAGCAGGCTGGTCTGCAGCCGGAAGGCCAGGAAGTCGGCGTCGCGGTGCATCAGGCGGTCCAGCTCGGGGCTCGGTGGCGTATGGAAGCGCGTCGGCTCGACCGGGGCGCCTCGCTTGCGAACGAGGTCTTCGAGCGTGTGTCCGGCGTTGACGACGGATCCGTCCGAGAAATTTTCGGCCAGGTGGCTTGATTCAGACGTGATGGATTTCGTCCAAAGCCGAACCATCTCGTCGGCCGGATCGTCGTCCGGATCGCCGCACGCCGTGATGAATTCCCGGACGCCGGCCCCTACCTGTTCGTAGAACCGGGCGCAGGCGGCGTCGAAGGATTGCGGATCCTTGGTGCGCAGATAAATCGCCTCGAAATGCGAACGGTAACTCAGCAGGCGTAGGGAAAGCAGCTCACGGAATTCGTATCCGTCGATTTCTCTCTGCGGTGATCTCAGAAGAGTGGCTCTGGTGTGCGCGGCCATGAGGCGGATGGCACCGAGTGCCGGCGGAGGCGATTGAGGTGTCTCTGCCGCACGATTCAAGAATGAGACGAGAGTCGGTGCGGCCTCCTGGAAGAGCCGCTTGGAGGAAGCGGAGAATCCGGTTCCGGTAAACCCCGCGTTCCACAACGGTGAGGGAACCGAATCAAGGGGCGTGGCGTCCACCTGTGCGGCGCACCCATATCGTTCGGCCGTTCTATGCAGGTCAGCGCGGGTGGAGTCCTCGTCGGACACCCCTTCGATGCTGATCTGCAGGGTGTCCGTGCCGAGCTGCGCGTCCTGGGTGCGGACGAAGTAGTAAGGGCCGTGACCGCCTGCTCCACCTACCCGCTCCGGCGCGAACAATTCGCGAATCAGGGGCGCGAACGTGTCGTTGTATTTTGAACCAACTGGCTGCGGGATGGTGGCAAACAAGTGCACGAATGAAACCCCCGAGATTTCGGCCGGCGCCAGACGGGCCGTCACGTGATCTTGTAATCGACCGTAACTCAGAGAGATGAGAAGAAGGAACCCCGGCTCCAGTGTGATCTGCGTCACTCCGGAAACTGCTTGCGTTGTGATCGTCCGAATGCCTAGATTCGAATCACATTGACGAAAGGGGGTGTAATCAATGGAGCAGCAGATCGAACTCGATGTGCTCGAGATTTCGGACCTCATTGCAGGTGCCGGGGAGAACGATGACCTGGCGCAGGTGATGGCCGCCTCGTGCACGACCACCAGTGTTTCGACGAGTTCTTCGTCGTCCTCGTCCTGAATCTAGGGACCGGGAAACAGTTGAGCCACCGTCGGGGTGTTCCTCGGCGGTGGCCTTCTGCAGTCCTGGCGTGCCTTTTTCGATTCGAGGAACGCGCCGTCCCTGGAGGTCGACCAGAGGTGCGGGCGTCGTGCCCCGGACGACGACGGGCTCGTGCCAAGCCGGCCGGCCGAGAGCCGGAACACATGTTGTCAAGCGCGAACTGACCGGTGCGGTGGAGTGACAGGCGGGCTCCGTGCGGCGGGGCGCCTCTCCGCGGCCCGGCCCCGACGTCCGCACGCCGTGGTGAACCGGGCCGGCGTGACGAAGTTGGGGGATTCCTATGGGTGTGAACATCAGTCCGTACGTCGTCTATCGGCGCAGCAGACTCCCACTGGGCGAGCTCGGAGGGATGTCCTTCACCACCGCCTGGTCGCGCATCGATGAACTGCACGCCCTGCGGGACGAGATCGGCAAGAACGCCGTCGGCCTGGCCGACCGCCTCGGCGAGCTCGTGCCTACGCTGGGGGACGACGTCCGGGCCGACCTGATCAGGCTGCGGCGCGACGTGCACAATCTGCGGCACGACCGGGCGGTGGCGCGACTGGAGCCACTGCGTCCGCATCTCGGCCGCGAGGTGGTCGACGAGGTCGAGACCTGGTGCGCGCTCGGCGTGCGGGCCGAACAGTGCGAGCGAGCAGGGCGCGAGGAGCTCGAGAGTGAGAAGGCCCGGGCCGCCGACGGCTTCGGCGCCCTCTTCGAGCACGATGCGATGGCGCGCAGCATCCAACTCTCCGGCGACCGGCTGTACCGGGGCCTGCGCGACCTCGTCGCGGGCGACGAGGCGAGCGCCCTCAAGCCGAGCAAGGCCCGGCTGCGGGAGTCTTCCCTCGTCAACTTCGCCTACCGGGCGAGCTTGAAGCCGTCCCCCTTCGGACGGTTCACCGAGATCGGCGCGTTCCCTCCGGACGACCCGCGCCCCGCGGATCCCGGTGGCCGGCACGGCGGGACGCAGGAGTCGGTCACGACGCTGAACCGTCTCCTCGTGAACTGGGGGCCCCCCGGCCTGCCGCTCGTACCGGGCGGGATGGAGCCGGGGCACCTCGTGCTGAACTCCACGCTGCGGGCCGGCACCGAGTACGTCGAGTACGTCGGTGTCGCTCCCGGCTCCCGTGAGGACGGCCGGATGGCCACCGAGAGGGTGCTGCGCGTACGCCGGGAGGGACTCTTCGACGCACTGCTCGCGGCGATGCCCGAAGGATCGGCTCCGGCGGCCACGGTGCTGCGCGACCTCACCGCCGTCACCGGGAAGGCGGAGACGAGCCGGAAGGTCGTGCAGGGGCTGATCCGGGCCGGCATCCTCTTCTTCCGGCCGGAGATCGACGATCACGACCCCGACTACTCCATGAAGCTCGACCGCGTACTCGCGGCCGGCGGGACGCCGGAGACGGCCGCGCTACGCGGACACTTCTCCGAACTCAGGCGGTTGGAGACGGACTTCTCCGAGGCGGCGGCCGACGAGAGGCAGAAGCTGCTCGACTCGGCGTACGCGGCGATCGGCGGCATCGCCGAGCTGTGCAAGGTGTCCCCGCCCCCCGAGGAGGTCCTGAAGTCACCGGTCTTCGAGGACACTCCGGCATCCACGGCGCCCCAGGCCTGGAACCTGCCGACGGTGGAGGGGAGCATCCCCGCCCTGACGGGCCTCTGGCGTCTGGCCTCGATGATGGACAACGGCCAGGTGAAGCGACTGGGTCTCTACTCCTTCGCCACCCGCGTGCTCGGCGACCGCAGCACGATGCCCTTCCTCGAGTTCTTCCAGGCCTTCTCGTCGCTGACGGACCAGGAACAGGTCGACGTGTTCATGGGGCGCGACGTGGAGGAGGCCGAGAGGTACACGAGGCAGCGGGCGGAGGCTCTGCGCACGATCCGGCAGCGGCTGGTGCCCGGGGACGGCACCGTGCACCTGGACCCCTCGGTCATCGAGAAGGCCTGCGAGGGCGTGGAGGACCTCCTCGACACGGAATCGGTGACGTTCCGCGCGCAGTTCGCCCAGGGAGTGCTGCCCGACCGGGACCGGACGTTGGTCGTGAACGGCCTGCTCACCGGCTACGGCGTCTACTTCTCACGGTTCGGCTCGTTCGTCGAGGGCACCGACGAATGGTCCCTGCCGGCCGCCCAGCGGGAGCACCTCGCACGCAGGTTCCCCGGCCAGGTCGACCTCAACTCCGTGCTCGGATTCAACTTCAACCTGCACCCCTCGGTGACCCGGCGGGTCGTCAACTACCCCGGCGCGGTGTCGCTCGGCGCCGAGCGGACGGTCTACGGACTGGCGCGTCTGGAGGTCCGCGCGGATCAGGCCACCAGGTCGCTGCGCCTCTGGGACCCTGAGGCGCAGGAAACCCTCGACCTCGTGCCCATGAACTTCATGACCCCGATCGGGGTCCCGCTGCTCTACCGTCTGCTCGAGGCGCTGTCCCCGTCCAACCGCTACCTGTGGAAGCCCCTGGACGACATCAGGGACGCGGGAGGGCCCACGGTGTACGGCGAGACGGCACCCCGGCTGGTCGTGGGTGACGTCGTGGCCGACCGCAGGTCCTGGAACGTGGCCGCGGCCGAGATCCCCATGCTCCAGGATCTGAGCCGGGACGTGCCCGAAGCGCTCGTGGCCTTCGACGCGTGGCGCCTGACGCGGGGCCTTCCCCGCCACGCCTTCGTGCTGTGCCAGACGCCCGAGGAGCGAGACGTCATGGCCGGGCGCAGCCGGAAGGTGACCCGCCAGTGGGCGGACTACGCGCACCTGCGGCGCGCCAGCGTGCACAAGCCGATGTACGTCGACTTCCGGAACCCCTTCCTGGTCCGGAGCTTCGCGAAGTCGGCCCTGTCACGCGGCGATGTCGTCGCGTCGATCCGCGAGTGCCTTCCTTCGGTGGACGACTACGGCCCGGACACGGGCTGGACCGCAGCAGAGGAGTTCTTCGTTGAACTGTGTACCGACAACTAGTGGGCAGACCGGAACGCGTGAGTGGAGGACGGTCCACATCCACGTTCCGCACTCGCTGCACACCCCCTTCCTGTGCGACGTGGTCGAGCCGCTGCTCCGGTCCGAGGGACTCCAGGACCACTTCTTCTTCCTCCGGTACTGGCAGGGCGGCCCCCATCTGCGGCTGCGGATGCTCTGCGGCCCCGGGGCCGGTTCGGCCGAGGCGGCCGAACGGGTCGTCGCGGGTCTGGCACGTGCGATGCCGGAGTTCGGTGCGCAGGCGCGGGAGGAATACGCGCTCGGGCTGACCTTGCAGGACGAGCTCGCCCGCCTGGAGAAGGAGACCTCGGAGGAGGGCCGGCCCATCGGGGCCCTCGACCGGGTGGCGTACGAGCCGGAGTACCGCAAGTACGGGGGAACGGAGGGGCTGCAGATCGCCGAGACCGTATTCCGCAAGTCGTCGGTGGCGGTCCTCGGCCTGCTGGGCGGGCAACCGCGGGCGTGGGTGGACGAGCGCCGGGCACCGATCGGGGAAGCCGCGAGGATCATGGCGATGTTCCTCCACGGCGCAGGCCTCGACCCGCGGGCCGCAGGGCTGTTCCTGCGGGAGTACGAGGACTGGTGGCGTACGTACGCGCCGGATGACATGCAGCGTGCCTGGCCGAAACTGTTCGGCGGCGTCTCGGCACAGATGACGAATCTGTGCGCGGCGGTCTGGCGTGACGGCGCCACGGACGTGTTCCACGACATCAGCGCGGAGGCCGCCGCCCGCGCCCGTTCCGTGTGCGGGGCGGAGCCCGGCGGCGATGTCCGCGACCTCCGGCTCGACGGCACGCCTTACCCGGGCTGTCTCTCGAACTACGTGCACACCACCAACAACCGTCTCGGCCTGGTCCCCGCCGCCGAGGGGCTCGTCGCGTACCTCGTGCGCCGGGGCCTGGAAGCGATGGACGGGTAGGGCCTGTCGTTGTCGGGATCATGCGGGGCCCCGATGCCCCGGTGCCGGAAGCCCGGAGCCTCCGTCCCGCGCAGGCCCTTCCCTGTGGGGCCTGCGCCGGACGGAGAGCTCACCGGTGCGTCACCAGCTCACCGGAAGCTTGTGCAGGCCGTACACCCCCATGCTGTCCTTGAACGGCAGTTCCGCGACCGGGGTGGCCAGCTTCAGTCCGGGAATCCTCGCGAGGAGGGCGTTGAAGACGACCTCCAGCTCCAGTTTCGCGAGGTTCTGGCCGATGCACTGATGGATGCCGTGGCCGAACGCCAGGTGGTGCCTGCCGCCGCGCTCGATGTCCAGCCTGTCCGGGTCGGGGAACACCGCCTCGTCGTGGTTGCCGGAGGCGCTGAGCCCGATGACGCCCTCACCCGCGCGGATCAGCACCCCGCCGATCTCGAGGTCGGCCGTCGCCACCCGGGAGGTCACCTGGTCGGCGATGCTGAAGTAGCGCAGCAGCTCGTCGACGGCCTGCGGGGCCAGACCCGGGTCGGCCCTGAGCTTGGCCAGCTGGTCGGGGTTCTCCAGCAGGCCCACGACGCCGAGGGAGATCATGTTCGCGGTGGTCTCGTGACCGCCGACCAGGAGGACGTTCGCCATCCCGACCAGTTCCCCGTGGTCGAAGGTGCCGGTCTCCCGGTTCTTCACGACGAGCCTGCCGAGCAGATCGTCCCCCGGGTCCGCTTCCTGAGCGGTGACCAGCTCGGAGAAGTACGCGTGGAGCTCTTGATGCGCGCTGTTGCGCTCCTGTGGGTCGGCGTCGACCGACACCAGCTTGTTGGTGCGGTCCTGGAAGAACGCGCGGTCGCTGCGGGGCACCCCGAGCAGCTCGCAGATCACCAGGGACGGCACCGGGAGGGCGAGCGCCTCGACCAGATCGGCCGGCCCCTCGCCGGCGAGCATGGAGGAGATGCACTCGTCCACGATCTCCTCGACGCGGGGACGCAGTTGGAGCACCCGCTTGACGGTGAACTCCGGGATCACCATCTTGCGCTGCGCCGTGTGTACGGGCGGGTCCATGGCGAGCAGCACGGGCCGCATCTGCTCCATGACCTCCGGCGGCGCATCGAAGTGGAGTGGATAGCCCGGGTGGGCCAGGTTCGAGCTCACATGAGGGTCGGCGAGCAACTGTCGTATGTGCTCGTGCCGGGTGAGCAGCCACGCGGTCCGCCCGGAGGCCAGAGTGACCTTGGTGATCGGCTGCTCGGCACGCAGGGACGCGTACTCCTTCGGCGGGTGGAGCGGGCAGGTCCTGGGGTAGGGATACGCGCTGTCCTGTCGGTCCGTCACGGTCTTCTCCGCGGGTAGTCCAGGGAAATCTCCTTACGGACTCCATTCAAGCGGAAGATGATCCACATCGTGGCGTTATCGGTGAATGAGGCCGAACTCACGTGGACCGCAGGGAGGAAAGTCGGCCGCCTTCCCGGCATGGCCCGTCCCGTGCTTTCGGTGGGAGGGGTCGGTGCAGTGCGACATTGCAGTGATCGCGTAATCCGGAATGACCCCTTCCGTGCGGGGATGCGTCGATAGTACGTTGGATTTCATGTGCTCCACATCGTGGAGAGTTCGCTTGCGCCGTCGAAGTCACAGTGTGTGCCGAGGGGGAGTTGGGGCTATGTACCTTTCGATCGTCATGTGGGACCTGAAGAAGTCGGAAGCCACGGTGGAGAGCCTCAGGGAATACCTGCGGGACTATGCCGTGGACGCCTACTCCGCGCTGGACGGAATGCGGCTCAAGGCGTGGTTCTCCGATTCCGCACGTCAGCTGTGGGGTGCGGTCTATCTGTGGGACAGCCCCGAGCAGATGCCCGGCCTGTACAAAGTCAGCCGCGTGATCGATCTCATCGGGTATCCGCCGACTTCGGTCGGTGGTTTCACGCTCGAGGCGACCGCCGAAGGGAAGAGCGTTCACGAGACACTGGCCGGCCTGGGGATCGCCCTGGAGGGCGGAACGCAGTAAGGGCAGGCGTGCGACCGGATGGGCGGGAGGTTTGCCGGGCCGGTCGGTTCGAGGGGCGGTCGAGGGCCGGAGCCGCGCGGGTGGCTTCGCCGCTCCGGCTGGCGGCCGGGTGTGGAGTGCCGCGCGGCGCCGGTGTCGCCCGCGCCGGGCATCACCGTGCTCGCGGAGGGTTCAGGTGTGTCGGACCGGCGCTTTCGCGTGTGTTCGGCACCGTCCGGTGGGCCGGGTGCTGTGCGCGGGCCTCGAGCTCCCGGGCGGGCGCGGTGCGCAGGCCCTCCCCGCCCGGACACGCGTATTCCGCACCGCTTCACGAAGATCATTCGGTGAAGGAGGCGGGGGCGCTCGTGCTAACGTCGTGATCGTGGCCAGCCTTGACATTATTACCGAACGCTCTGATTCTGCCGTACAACGCATCATCGATGTGACAAAGCATTCGAGGTCCGTTGTCCGCACGGTGCTGATCGAGGACATCGAGCCTCTTCTGCAGAGCATCCGTGCCGGAGTGGAATTCATCGAGATCTACGGACTCGACACCGTGCCTGTTCCGGACAGTCTGCTCGCCGAATGTGAACGGCGCAGAATTCCGGTCCGGCTGCTCGCCGCTTCGGTCGCCAATCAGGTCTTCAAGACCGAGAAGAAGCCCAAGGTATTCGGTATCGCCAAGGTCCCGCGGCCTCGTCGCCTGTCGGACCTGTCCGACATGACCGGTGACCTCATCCTGCTCGACGGAGTGAAGATCGTCGGCAATATCGGAGCCATCGTGCGGACCTCGTTCGCGCTCGGGGCCTCGGGAATCGTGCTCGTGGACAGCGATCTCGGCAGTATTGCGGACCGCCGTCTGATCAGGGCGAGCCGGGGCTATGTGTTCTCCCTTCCCATCGTTCTCGCGTCCCGGGCCGAGGCGCTCCAGTACTTCCAGGACAATGCGATGCGCCCGGTGGTGTTCGAGGCCGACGGGGATCTCGGCGTCGCTGATCTCGACGGTATGGACGAGCGACTTGTGCTCATGTTCGGCAGCGAGAGGATCGGCCCGTCGGGCGAGTTCTCCGACATCGCCGCCAAGTCGGTCTCCATTCCGATGAATCCCGCGGCCGAGTCCCTCAACGTATCGGTGTCGGCCGGAATCGCGCTGCACGCGAGGGCCCGCCGTAACCTCTCCCGGTAGTCCCGGCCGCAGAGCCCCGTCAAGGGCCCCGTCCCTCCCCTCCGGGAGGGGCGGGGCCCTTGACGTGCGCCGCCGGGAGCCCTCGCTACCGCGGCCGCCACATCCAGGGCGCCGCGTCCGGACCCAGGCCGGCGACCGAAGGCCGGCCGTCCGGGCCCAGGCGGAGCGAGGCCCCGTCCAGGGCGGCCGGCCCGTCGGTCCGCACGTGGAGCCCGCCGCCCATGAGCAGCTGGACGAGGCCCTCGGCGGTGCGGCCCAGCAGCACGGGCCCCCGCGGCGACGGGGCGGCGTCCACCGNNCCGTACCCGTCGAACCGTGCTCCGGGCACGGCCGTCCCCGCCCGAGCCGTGGTCAGCCCGCTCCCGGCCGCTGCCCGGTAGTAGAGGGACACGGACCCGTCGGGAGCCGGGAGGGCGGCCGGCGCGTGGGCCGGGACGGGCGCCGCCGTGAGCTGTGTGCGAGCCGTGAGCCCGGCGGACGGGGTGTCCTGGGTCCAGTGGTGTACGGCGTGGTGGCCGGCGCCGAAGACGTGGACGCGTCCACCCTCGTCCACGGCGGTGTGCAGGCCGTCCTGCACCTCTCCGCCGCCCATGTCCCGCCACGCGCTCCACCGCCCCGCCCCGTCCCGCACCCGGGTGCTGACGCCCTTCTCGGCGTCGCGTACGAAGAGATGGATCTGCCCGTCCGGAGCGGCGACGGCCACCGGTACGCCCGTGCGACGTACTTCGTCACGTCCGGGAGAAGGAGAGCCCAGGCCGCGCCAGGGGCGGAAGCCCCGGCCCGGGGCGCTCTGCTCCAGCACCACGATCTCGCGCTCGTTGTCGGCGCCGTGCCCGCCGAGTGCGGCGAAGCGGAGCCCGAACAGCAGCAGGCGCCCGTCCCGTGTGGTGGCCGAGCCCAGTGCGGGGGCGAGCGGGCCGCCGCCTAGGTCGTGCGGAGGCCCCCAGGCACCGCTGCCCGGCCCGGTCTCCTGCCACCGCACCACCCGCAGCCCCAGCACGGCATAGGCCGCGAGCCTGCCGTCCGGCTCGGCGGTGAGGACCGTGCGCGTGCCCGGGTAGCGGTGGTGGGTGGAGCGGACCCAGCCCTTGCGGTTGGTGAGCGGGCGGTCGCCGCCGACGTTGTAGTCACCGCAGCCGGACGGATTGCCGCAGTCCCAGTCGGGCGAGCCCCCGTAGGGGACGAGGTGGGCGGCCTTCCTCGCCAGTACCCCCTGCGGCAGGTTCTTCGGCCAGTGCCGGTTGTAGTAACCGCGGTAGGCGACCGTGACGAAGCCCGGTATCCGGCCACCGTCCGCGGTCGCCCGGGCCACCCAGCGGATCATCGCGGCCCACGCGAAGCAGGCCGCGGCCGTGTGGTCGGCGTGGTCGGAGTAGCCGGGCTGTTCGCTGTCCCTGCGGCGGACGGCCTCCGTGCTGTGCTGGATGTCCGGGTCGGGGTCCAGGGTGTGGACGACGGTGGGCCGGTAGCTCTCCATCAGGCCGGTGAGGACCCCGACCAGCCCGTCGTACGTGTACGAGCCGGCGCGCCGCAGCGGCGATCCGTCGGCCACGACCGTACGCAGCACGAGCCGGCGATCCTGCCAGAGACTGGGCAGCCCGAGCCGGTGCCGGCCGGTGTGCATGGCCGTGTTGAGGAAGATCAACTCCACTCTGCGGCCGTCGGCCGCCAGCACGTTCACCTCGGCCCTGTGGTCCGGGCCGAGCTCGGCGACGGAGACCTCCCAGGGGGTGAAGGGCCGCAGCCCGAGCAGGGCG

test/files/.ipynb_checkpoints/BGC0001472.fna.prodigal-checkpoint.faa

+>BGC0001472_1 # 312 # 683 # 1 # ID=1_1;partial=00;start_type=GTG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.642
+MPTIQQLVRKGRQDKVEKNKTPALEGSPQRRGVCTRVFTTTPKKPNSALRKVARVRLTSG
+IEVTAYIPGEGHNLQEHSIVLVRGGRVKDLPGVRYKIIRGSLDTQGVKNRKQARSRYGAK
+KEK
+>BGC0001472_2 # 686 # 1156 # 1 # ID=1_2;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.660
+MPRKGPAPKRPVIIDPVYSSPLVTSLINKILLDGKRSTAERIVYGAMEGLREKTGADPVI
+TLKRALENVKPSLEVKSRRVGGATYQVPIEVKPGRAATLALRWVVGYSRARREKTMTERL
+MNELLDASNGLGAAVKKREDTHKMAESNKAFAHYRW
+>BGC0001472_3 # 1195 # 3324 # 1 # ID=1_3;partial=00;start_type=ATG;rbs_motif=AGxAG;rbs_spacer=5-10bp;gc_cont=0.652
+MATTSLDLAKVRNIGIMAHIDAGKTTTTERILFYTGVSYKIGEVHDGAATMDWMEQEQER
+GITITSAATTCHWPLNDVDHTINIIDTPGHVDFTVEVERSLRVLDGAVTVFDGVAGVEPQ
+SETVWRQADRYGVPRICFVNKLDRTGADFLRCVDMIVQRLGAVPIVMQLPIGAEADFRGV
+VDLVSMKAFVYPEEAVKGEMYDTVEIPDNLKEAAEEWRGKLLEAVSENDDQMMELYLEGE
+EPTEEQLHEAIRRITLASKGSADSVTVTPVFCGTAFKNKGVQPLLDAVVRYLPSPLDVEA
+IEGHDVKDPEKVVQRKPSDDEPFSGLAFKIASDPHLGKLTFVRIYSGRLEAGTAVLNSVK
+GKKERIGKIYRMHANKREEIPSVGAGDIVAVMGLKQTTTGETLCDDKNPVILESMDFPAP
+VIQVAIEPKSKGDQEKLGVAIQRLSEEDPSFQVHSDEETGQTIIGGMGELHLEVLVDRMK
+REFRVEANVGKPQVAYRETIRKAVERIDYTHKKQTGGTGQFAKVQIAIEPIEGGDASYEF
+VNKVTGGRIPREYIPSVDAGAQEAMQFGILAGYEMVGVRVTLLDGGYHEVDSSELAFKIA
+GSQAFKEGARKASPVLLEPMMAVEVTTPEDYMGEVVGDINSRRGQIQAMEERHGARVVKG
+LVPLSEMFGYVGDLRSKTSGRASYSMQFDSYAEVPRNVAEEIIAKAKGE
+>BGC0001472_4 # 3472 # 4665 # 1 # ID=1_4;partial=00;start_type=GTG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.642
+MAKAKFERTKPHVNIGTIGHIDHGKTTLTAAITKVLHDAYPDLNEASAFDQIDKAPEERQ
+RGITISIAHVEYQTESRHYAHVDCPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTKE
+HVLLARQVGVPYIVVALNKADMVDDEEILELVELEVRELLSEYEFPGDDLPVVKVSALKA
+LEGDAEWGQTVLDLMKAVDESIPQPERDVEKPFLMPIEDVFTITGRGTVVTGRIERGVLK
+VNETVDIVGIKTEKTTTTVTGIEMFRKLLDEGQAGENVGLLLRGIKREDVERGQVIIKPG
+SVTPHTEFQAQAYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNT
+LMDVALIQPVAMEEGLKFAIREGGRTVGAGQVTKITK
+>BGC0001472_5 # 4869 # 5570 # -1 # ID=1_5;partial=00;start_type=ATG;rbs_motif=GGAGG;rbs_spacer=5-10bp;gc_cont=0.712
+MRNDVTSMTAVLEGFTSRTPTSDGLAAERRPVPFADSVPVEPQPSAEDLRPVHDLRGTLE
+RRRSSLHYAPLPVRTDVILSLLRDVLRRDRDDWGLDASAGALEGFVFAFRSEGAEPGLYR
+VTAEETCYLAGLDEIGPAENLGVQREFSTGAGIVALYASLDRADTWAGSHGYRISALRAS
+MATYDLNLRCQALGLVGTLFGGFVPSSVHHLVHSDGATRHSLLATTYARPPES
+>BGC0001472_6 # 5567 # 7195 # -1 # ID=1_6;partial=00;start_type=ATG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.709
+MVAEMKAEQIGRAARTDMQLTVPARPVLRRGVRLRRAGESVVLDGADRAQVFSGAFAREG
+LVPLTEACDGTRDHTELALKTGFDEATVYKCLALLSTAGAVEEAMSGEEPDVTPEWAVFL
+SRLGNSTGSNPSWADAAARLVSRSVRLEGDAALVAGARRSLREVCPVVTEPAGPPGPGDE
+LTVFFETPASAPLLAATEERCRQDGRPLLRVRADARTITIGPYADLSITPCLDCGRHGEA
+DLSGEPPEYLHDLVVGLASHHVTALLARATISHLPGDFTVIDTATLSTVYRPVAVRPGCP
+RCSYARGPVAPQAPAGAVYEASVAMPPRAFLAPKDHQAHYYASNLRLQSQFKDWPSRPHT
+PLPALDISVLAGSERHDPSHGDTPLTLSSLGLLLKVAFGVKEDETTPERVKRWTAASGNI
+GSTTAYAVVRDDRIMPPGVYAYAQGSHTLVTVSGEVPPGDSPCDIIITGDLKKVMTKYGT
+FGFRLVFLDAGCNLASLRELAQHLGLGFTPRSDWDDDALARLLGTSPADEPVAAFASLGG
+TA
+>BGC0001472_7 # 7210 # 7821 # -1 # ID=1_7;partial=00;start_type=ATG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.712
+MSHDPRPQCLYLVGDTFSRRLTEHRGVPPELQVSFEDFLNDTAPHADVVVPVHAGGDPGL
+RDETDRICAERSTPSVGLQLLPTKVLCGPVVVPGRTACYACYRKRAAQHAGTARPYDMDA
+ALSGLPEGFGRQHLSVASGLLDLALTEIATGVTGIGGTVRTFNLVSGAVSSAVTVSVNRC
+PRCGGRFSQARADSAMPVPELLR
+>BGC0001472_8 # 7845 # 9191 # -1 # ID=1_8;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=11-12bp;gc_cont=0.709
+MHLNRPQEHISAELRGLEELVSPYGLVSRTAPLPVREGEPPFAVQLAYLGVPSRALPNLR
+TWAHDEDTGNSDGAGTGLTPERAKLVSIAEALERYSTCAWDDDEMVVAAENDLTEEFVSP
+SRWPSCSPTELARDDCSLSAYDPSVPIRWVRAWSLTRRIPVLVPAISVYLHMPYQSKSEE
+FIRGITTGAAVHSDVRSAVLGGLLEVVERDAIALVWLQQLRLPELVVDPARLDAGVRELH
+RVGTSTDLRVRLFDATTDFGVPVIYAVQLSDADPALAQIVAATCDVHPEQALGKIYRELA
+SLRVALRGYLSAYAGREPDPAKVSVVGGAVHNATRDRRDVFGFLLDGERPAYGLEGMPGL
+PAGADPLDTVVARLAARGAEVLVTDITTDEARQVGMRAVKVLVPEAMPVSFVHGERYLGT
+PRLYDAPRAMGHTSHAEDAVNPVQQPFA
+>BGC0001472_9 # 9238 # 10437 # -1 # ID=1_9;partial=00;start_type=GTG;rbs_motif=AGxAG;rbs_spacer=5-10bp;gc_cont=0.621
+MTQITLEPGFLLLISLSYGRLQDHVTARLAPAEISGVSFVHLFATIPQPVGSKYNDTFAP
+LIRELFAPERVGGAGGHGPYYFVRTQDAQLGTDTLQISIEGVSDEDSTRADLHRTAERYG
+CAAQVDATPLDSVPSPLWNAGFTGTGFSASSKRLFQEAAPTLVSFLNRAAETPQSPPPAL
+GAIRLMAAHTRATLLRSPQREIDGYEFRELLSLRLLSYRSHFEAIYLRTKDPQSFDAACA
+RFYEQVGAGVREFITACGDPDDDPADEMVRLWTKSITSESSHLAENFSDGSVVNAGHTLE
+DLVRKRGAPVEPTRFHTPPSPELDRLMHRDADFLAFRLQTSLLYSCLYTLGFSLAERYVF
+CYVVARANEDVCGKSMKELQDELDGLARSMASGSTKTAE
+>BGC0001472_10 # 10511 # 10654 # 1 # ID=1_10;partial=00;start_type=ATG;rbs_motif=AGxAGG/AGGxGG;rbs_spacer=5-10bp;gc_cont=0.590
+MEQQIELDVLEISDLIAGAGENDDLAQVMAASCTTTSVSTSSSSSSS
+>BGC0001472_11 # 10977 # 13634 # 1 # ID=1_11;partial=00;start_type=ATG;rbs_motif=GGA/GAG/AGG;rbs_spacer=5-10bp;gc_cont=0.701
+MGVNISPYVVYRRSRLPLGELGGMSFTTAWSRIDELHALRDEIGKNAVGLADRLGELVPT
+LGDDVRADLIRLRRDVHNLRHDRAVARLEPLRPHLGREVVDEVETWCALGVRAEQCERAG
+REELESEKARAADGFGALFEHDAMARSIQLSGDRLYRGLRDLVAGDEASALKPSKARLRE
+SSLVNFAYRASLKPSPFGRFTEIGAFPPDDPRPADPGGRHGGTQESVTTLNRLLVNWGPP
+GLPLVPGGMEPGHLVLNSTLRAGTEYVEYVGVAPGSREDGRMATERVLRVRREGLFDALL
+AAMPEGSAPAATVLRDLTAVTGKAETSRKVVQGLIRAGILFFRPEIDDHDPDYSMKLDRV
+LAAGGTPETAALRGHFSELRRLETDFSEAAADERQKLLDSAYAAIGGIAELCKVSPPPEE
+VLKSPVFEDTPASTAPQAWNLPTVEGSIPALTGLWRLASMMDNGQVKRLGLYSFATRVLG
+DRSTMPFLEFFQAFSSLTDQEQVDVFMGRDVEEAERYTRQRAEALRTIRQRLVPGDGTVH
+LDPSVIEKACEGVEDLLDTESVTFRAQFAQGVLPDRDRTLVVNGLLTGYGVYFSRFGSFV
+EGTDEWSLPAAQREHLARRFPGQVDLNSVLGFNFNLHPSVTRRVVNYPGAVSLGAERTVY
+GLARLEVRADQATRSLRLWDPEAQETLDLVPMNFMTPIGVPLLYRLLEALSPSNRYLWKP
+LDDIRDAGGPTVYGETAPRLVVGDVVADRRSWNVAAAEIPMLQDLSRDVPEALVAFDAWR
+LTRGLPRHAFVLCQTPEERDVMAGRSRKVTRQWADYAHLRRASVHKPMYVDFRNPFLVRS
+FAKSALSRGDVVASIRECLPSVDDYGPDTGWTAAEEFFVELCTDN
+>BGC0001472_12 # 13612 # 14571 # 1 # ID=1_12;partial=00;start_type=TTG;rbs_motif=AGGAG;rbs_spacer=5-10bp;gc_cont=0.706
+MNCVPTTSGQTGTREWRTVHIHVPHSLHTPFLCDVVEPLLRSEGLQDHFFFLRYWQGGPH
+LRLRMLCGPGAGSAEAAERVVAGLARAMPEFGAQAREEYALGLTLQDELARLEKETSEEG
+RPIGALDRVAYEPEYRKYGGTEGLQIAETVFRKSSVAVLGLLGGQPRAWVDERRAPIGEA
+ARIMAMFLHGAGLDPRAAGLFLREYEDWWRTYAPDDMQRAWPKLFGGVSAQMTNLCAAVW
+RDGATDVFHDISAEAAARARSVCGAEPGGDVRDLRLDGTPYPGCLSNYVHTTNNRLGLVP
+AAEGLVAYLVRRGLEAMDG
+>BGC0001472_13 # 14692 # 15894 # -1 # ID=1_13;partial=00;start_type=GTG;rbs_motif=GGAG/GAGG;rbs_spacer=5-10bp;gc_cont=0.685
+MTDRQDSAYPYPRTCPLHPPKEYASLRAEQPITKVTLASGRTAWLLTRHEHIRQLLADPH
+VSSNLAHPGYPLHFDAPPEVMEQMRPVLLAMDPPVHTAQRKMVIPEFTVKRVLQLRPRVE
+EIVDECISSMLAGEGPADLVEALALPVPSLVICELLGVPRSDRAFFQDRTNKLVSVDADP
+QERNSAHQELHAYFSELVTAQEADPGDDLLGRLVVKNRETGTFDHGELVGMANVLLVGGH
+ETTANMISLGVVGLLENPDQLAKLRADPGLAPQAVDELLRYFSIADQVTSRVATADLEIG
+GVLIRAGEGVIGLSASGNHDEAVFPDPDRLDIERGGRHHLAFGHGIHQCIGQNLAKLELE
+VVFNALLARIPGLKLATPVAELPFKDSMGVYGLHKLPVSW
+>BGC0001472_14 # 16220 # 16564 # 1 # ID=1_14;partial=00;start_type=ATG;rbs_motif=AGxAGG/AGGxGG;rbs_spacer=5-10bp;gc_cont=0.626
+MYLSIVMWDLKKSEATVESLREYLRDYAVDAYSALDGMRLKAWFSDSARQLWGAVYLWDS
+PEQMPGLYKVSRVIDLIGYPPTSVGGFTLEATAEGKSVHETLAGLGIALEGGTQ
+>BGC0001472_15 # 17019 # 17729 # 1 # ID=1_15;partial=00;start_type=GTG;rbs_motif=3Base/5BMM;rbs_spacer=13-15bp;gc_cont=0.636
+MLIEDIEPLLQSIRAGVEFIEIYGLDTVPVPDSLLAECERRRIPVRLLAASVANQVFKTE
+KKPKVFGIAKVPRPRRLSDLSDMTGDLILLDGVKIVGNIGAIVRTSFALGASGIVLVDSD
+LGSIADRRLIRASRGYVFSLPIVLASRAEALQYFQDNAMRPVVFEADGDLGVADLDGMDE
+RLVLMFGSERIGPSGEFSDIAAKSVSIPMNPAAESLNVSVSAGIALHARARRNLSR
+>BGC0001472_16 # 17815 # 19485 # -1 # ID=1_16;partial=01;start_type=Edge;rbs_motif=None;rbs_spacer=None;gc_cont=0.756
+ALLGLRPFTPWEVSVAELGPDHRAEVNVLAADGRRVELIFLNTAMHTGRHRLGLPSLWQD
+RRLVLRTVVADGSPLRRAGSYTYDGLVGVLTGLMESYRPTVVHTLDPDPDIQHSTEAVRR
+RDSEQPGYSDHADHTAAACFAWAAMIRWVARATADGGRIPGFVTVAYRGYYNRHWPKNLP
+QGVLARKAAHLVPYGGSPDWDCGNPSGCGDYNVGGDRPLTNRKGWVRSTHHRYPGTRTVL
+TAEPDGRLAAYAVLGLRVVRWQETGPGSGAWGPPHDLGGGPLAPALGSATTRDGRLLLFG
+LRFAALGGHGADNEREIVVLEQSAPGRGFRPWRGLGSPSPGRDEVRRTGVPVAVAAPDGQ
+IHLFVRDAEKGVSTRVRDGAGRWSAWRDMGGGEVQDGLHTAVDEGGRVHVFGAGHHAVHH
+WTQDTPSAGLTARTQLTAAPVPAHAPAALPAPDGSVSLYYRAAAGSGLTTARAGTAVPGA
+RFDGYXXVDAAPSPRGPVLLGRTAEGLVQLLMGGGLHVRTDGPAALDGASLRLGPDGRPS
+VAGLGPDAAPWMWRPR