Skip to content
Snippets Groups Projects
ConversionSupport.pm 55 KiB
Newer Older
1001 1002 1003 1004 1005 1006 1007 1008 1009 1010 1011 1012 1013 1014 1015 1016 1017 1018 1019 1020 1021 1022 1023 1024 1025 1026 1027 1028 1029 1030 1031 1032 1033 1034 1035 1036 1037 1038 1039 1040 1041 1042 1043 1044 1045 1046 1047 1048 1049 1050 1051 1052 1053 1054 1055 1056 1057 1058 1059 1060 1061 1062 1063 1064 1065 1066 1067 1068 1069 1070 1071 1072 1073 1074 1075 1076 1077 1078 1079 1080 1081 1082 1083 1084 1085 1086 1087 1088 1089 1090 1091 1092 1093 1094 1095 1096 1097 1098 1099 1100 1101 1102 1103 1104 1105 1106 1107 1108 1109 1110 1111 1112 1113 1114 1115 1116 1117 1118 1119 1120 1121 1122 1123 1124 1125 1126 1127 1128 1129 1130 1131 1132 1133 1134 1135 1136 1137 1138 1139 1140 1141 1142 1143 1144 1145 1146 1147 1148 1149 1150 1151 1152 1153 1154 1155 1156 1157 1158 1159 1160 1161 1162 1163 1164 1165 1166 1167 1168 1169 1170 1171 1172 1173 1174 1175 1176 1177 1178 1179 1180 1181 1182 1183 1184 1185 1186 1187 1188 1189 1190 1191 1192 1193 1194 1195 1196 1197 1198 1199 1200 1201 1202 1203 1204 1205 1206 1207 1208 1209 1210 1211 1212 1213 1214 1215 1216 1217 1218 1219 1220 1221 1222 1223 1224 1225 1226 1227 1228 1229 1230 1231 1232 1233 1234 1235 1236 1237 1238 1239 1240 1241 1242 1243 1244 1245 1246 1247 1248 1249 1250 1251 1252 1253 1254 1255 1256 1257 1258 1259 1260 1261 1262 1263 1264 1265 1266 1267 1268 1269 1270 1271 1272 1273 1274 1275 1276 1277 1278 1279 1280 1281 1282 1283 1284 1285 1286 1287 1288 1289 1290 1291 1292 1293 1294 1295 1296 1297 1298 1299 1300 1301 1302 1303 1304 1305 1306 1307 1308 1309 1310 1311 1312 1313 1314 1315 1316 1317 1318 1319 1320 1321 1322 1323 1324 1325 1326 1327 1328 1329 1330 1331 1332 1333 1334 1335 1336 1337 1338 1339 1340 1341 1342 1343 1344 1345 1346 1347 1348 1349 1350 1351 1352 1353 1354 1355 1356 1357 1358 1359 1360 1361 1362 1363 1364 1365 1366 1367 1368 1369 1370 1371 1372 1373 1374 1375 1376 1377 1378 1379 1380 1381 1382 1383 1384 1385 1386 1387 1388 1389 1390 1391 1392 1393 1394 1395 1396 1397 1398 1399 1400 1401 1402 1403 1404 1405 1406 1407 1408 1409 1410 1411 1412 1413 1414 1415 1416 1417 1418 1419 1420 1421 1422 1423 1424 1425 1426 1427 1428 1429 1430 1431 1432 1433 1434 1435 1436 1437 1438 1439 1440 1441 1442 1443 1444 1445 1446 1447 1448 1449 1450 1451 1452 1453 1454 1455 1456 1457 1458 1459 1460 1461 1462 1463 1464 1465 1466 1467 1468 1469 1470 1471 1472 1473 1474 1475 1476 1477 1478 1479 1480 1481 1482 1483 1484 1485 1486 1487 1488 1489 1490 1491 1492 1493 1494 1495 1496 1497 1498 1499 1500 1501 1502 1503 1504 1505 1506 1507 1508 1509 1510 1511 1512 1513 1514 1515 1516 1517 1518 1519 1520 1521 1522 1523 1524 1525 1526 1527 1528 1529 1530 1531 1532 1533 1534 1535 1536 1537 1538 1539 1540 1541 1542 1543 1544 1545 1546 1547 1548 1549 1550 1551 1552 1553 1554 1555 1556 1557 1558 1559 1560 1561 1562 1563 1564 1565 1566 1567 1568 1569 1570 1571 1572 1573 1574 1575 1576 1577 1578 1579 1580 1581 1582 1583 1584 1585 1586 1587 1588 1589 1590 1591 1592 1593 1594 1595 1596 1597 1598 1599 1600 1601 1602 1603 1604 1605 1606 1607 1608 1609 1610 1611 1612 1613 1614 1615 1616 1617 1618 1619 1620 1621 1622 1623 1624 1625 1626 1627 1628 1629 1630 1631 1632 1633 1634 1635 1636 1637 1638 1639 1640 1641 1642 1643 1644 1645 1646 1647 1648 1649 1650 1651 1652 1653 1654 1655 1656 1657 1658 1659 1660 1661 1662 1663 1664 1665 1666 1667 1668 1669 1670 1671 1672 1673 1674 1675 1676 1677 1678 1679 1680 1681 1682 1683 1684 1685 1686 1687 1688 1689 1690 1691 1692 1693 1694 1695 1696 1697 1698 1699 1700 1701 1702 1703 1704 1705 1706 1707 1708 1709 1710 1711 1712 1713 1714 1715 1716 1717 1718 1719 1720 1721 1722 1723 1724 1725 1726 1727 1728 1729 1730 1731 1732 1733 1734 1735 1736 1737 1738 1739 1740 1741 1742 1743 1744 1745 1746 1747 1748 1749 1750 1751 1752 1753 1754 1755 1756 1757 1758 1759 1760 1761 1762 1763 1764 1765 1766 1767 1768 1769 1770 1771 1772 1773 1774 1775 1776 1777 1778 1779 1780 1781 1782 1783 1784 1785 1786 1787 1788 1789 1790 1791 1792 1793 1794 1795 1796 1797 1798 1799 1800 1801 1802 1803 1804 1805 1806 1807 1808 1809 1810 1811 1812 1813 1814 1815 1816 1817 1818 1819 1820 1821 1822 1823 1824 1825 1826 1827 1828 1829 1830 1831 1832 1833 1834 1835 1836 1837 1838 1839 1840 1841 1842 1843 1844 1845 1846 1847 1848 1849 1850 1851 1852 1853 1854 1855 1856 1857
  $self->throw("Could not determine species scientific name from database.")
    unless $species;
  return $species;
}

=head2 species

  Arg[1]      : (optional) String $species - species name to set
  Example     : my $species = $support->species;
                my $url = "http://vega.sanger.ac.uk/$species/";
  Description : Getter/setter for species name (Genus_species). If not set, it's
                determined from database's meta table
  Return type : String - species name
  Exceptions  : none
  Caller      : general

=cut

sub species {
  my $self = shift;
  $self->{'_species'} = shift if (@_);
  # get species name from database if not set
  unless ($self->{'_species'}) {
    $self->{'_species'} = join('_',
			       split(/ /, $self->get_species_scientific_name));
  }
  return $self->{'_species'};
}

=head2 sort_chromosomes

  Arg[1]      : (optional) Hashref $chr_hashref - Hashref with chr_name as keys
  Example     : my $chr = { '6-COX' => 1, '1' => 1, 'X' => 1 };
                my @sorted = $support->sort_chromosomes($chr);
  Description : Sorts chromosomes in an intuitive way (numerically, then
                alphabetically). If no chromosome hashref is passed, it's
                retrieve by calling $self->get_chrlength()
  Return type : List - sorted chromosome names
  Exceptions  : thrown if no hashref is provided
  Caller      : general

=cut

sub sort_chromosomes {
  my ($self, $chr_hashref) = @_;
  $chr_hashref = $self->get_chrlength unless ($chr_hashref);
  throw("You have to pass a hashref of your chromosomes")
    unless ($chr_hashref and ref($chr_hashref) eq 'HASH');
  return (sort _by_chr_num keys %$chr_hashref);
}

=head2 _by_chr_num

  Example     : my @sorted = sort _by_chr_num qw(X, 6-COX, 14, 7);
  Description : Subroutine to use in sort for sorting chromosomes. Sorts
                numerically, then alphabetically
  Return type : values to be used by sort
  Exceptions  : none
  Caller      : internal ($self->sort_chromosomes)

=cut

sub _by_chr_num {
  my @awords = split /-/, $a;
  my @bwords = split /-/, $b;

  my $anum = $awords[0];
  my $bnum = $bwords[0];

  if ($anum !~ /^[0-9]*$/) {
    if ($bnum !~ /^[0-9]*$/) {
      return $anum cmp $bnum;
    } else {
      return 1;
    }
  }
  if ($bnum !~ /^[0-9]*$/) {
    return -1;
  }

  if ($anum <=> $bnum) {
    return $anum <=> $bnum;
  } else {
    if ($#awords == 0) {
      return -1;
    } elsif ($#bwords == 0) {
      return 1;
    } else {
      return $awords[1] cmp $bwords[1];
    }
  }
}

=head2 split_chromosomes_by_size

  Arg[1]      : (optional) Int $cutoff - the cutoff in bp between small and
                large chromosomes
  Arg[2]      : (optional) Boolean to include duplicate regions, ie PAR or not
                (default is no)
  Arg[3]      : (optional) Coordsystem version to retrieve

  Example     : my $chr_slices = $support->split_chromosomes_by_size;
                foreach my $block_size (keys %{ $chr_slices }) {
                    print "Chromosomes with blocksize $block_size: ";
                    print join(", ", map { $_->seq_region_name }
                                        @{ $chr_slices->{$block_size} });
                }
  Description : Determines block sizes for storing DensityFeatures on
                chromosomes, and return slices for each chromosome. The block
                size is determined so that you have 150 bins for the smallest
                chromosome over 5 Mb in length. For chromosomes smaller than 5
                Mb, an additional smaller block size is used to yield 150 bins
                for the overall smallest chromosome. This will result in
                reasonable resolution for small chromosomes and high
                performance for big ones.
  Return type : Hashref (key: block size; value: Arrayref of chromosome
                Bio::EnsEMBL::Slices)
  Exceptions  : none
  Caller      : density scripts

=cut

sub split_chromosomes_by_size {
  my $self   = shift;
  my $cutoff = shift || 5000000;
  my $dup    = shift || 0;
  my $cs_version = shift;
  my $slice_adaptor = $self->dba->get_SliceAdaptor;
  my $top_slices;
  if ($self->param('chromosomes')) {
    foreach my $chr ($self->param('chromosomes')) {
      push @{ $top_slices }, $slice_adaptor->fetch_by_region('chromosome', $chr);
    }
  } else {
    $top_slices = $slice_adaptor->fetch_all('chromosome',$cs_version,0,$dup);
  }

  my ($big_chr, $small_chr, $min_big_chr, $min_small_chr);
  foreach my $slice (@{ $top_slices }) {
    next if ($slice->length eq 10000); #hack for chrY pseudoslice
    if ($slice->length < $cutoff) {
      if (! $min_small_chr or ($min_small_chr > $slice->length)) {
	$min_small_chr = $slice->length;
      }
      # push small chromosomes onto $small_chr
      push @{ $small_chr }, $slice;
    }
    elsif (! $min_big_chr or ($min_big_chr > $slice->length) ){
      $min_big_chr = $slice->length;
    }
    # push _all_ chromosomes onto $big_chr
    push @{ $big_chr }, $slice;
  }
  my $chr_slices;
  $chr_slices->{int($min_big_chr/150)}   = $big_chr if $min_big_chr;
  $chr_slices->{int($min_small_chr/150)} = $small_chr if $min_small_chr;
  return $chr_slices;
}

=head2 log

  Arg[1]      : String $txt - the text to log
  Arg[2]      : Int $indent - indentation level for log message
  Example     : my $log = $support->log_filehandle;
                $support->log('Log foo.\n', 1);
  Description : Logs a message to the filehandle initialised by calling
                $self->log_filehandle(). You can supply an indentation level
                to get nice hierarchical log messages.
  Return type : true on success
  Exceptions  : thrown when no filehandle can be obtained
  Caller      : general

=cut

sub log {
  my ($self, $txt, $indent) = @_;
  $indent ||= 0;

  # strip off leading linebreaks so that indenting doesn't break
  $txt =~ s/^(\n*)//;

  $txt = $1."    "x$indent . $txt;
  my $fh = $self->{'_log_filehandle'};
  throw("Unable to obtain log filehandle") unless $fh;
  print $fh "$txt";
  return(1);
}

=head2 log_warning

  Arg[1]      : String $txt - the warning text to log
  Arg[2]      : Int $indent - indentation level for log message
  Arg[3]      : Bool - add a line break before warning if true
  Example     : my $log = $support->log_filehandle;
                $support->log_warning('Log foo.\n', 1);
  Description : Logs a message via $self->log and increases the warning counter.
  Return type : true on success
  Exceptions  : none
  Caller      : general

=cut

sub log_warning {
  my ($self, $txt, $indent, $break) = @_;
  $txt = "WARNING: " . $txt;
  $txt = "\n$txt" if ($break);
  $self->log($txt, $indent);
  $self->{'_warnings'}++;
  return(1);
}

=head2 log_error

  Arg[1]      : String $txt - the error text to log
  Arg[2]      : Int $indent - indentation level for log message
  Example     : my $log = $support->log_filehandle;
                $support->log_error('Log foo.\n', 1);
  Description : Logs a message via $self->log and exits the script.
  Return type : none
  Exceptions  : none
  Caller      : general

=cut

sub log_error {
  my ($self, $txt, $indent) = @_;
  $txt = "ERROR: ".$txt;
  $self->log($txt, $indent);
  $self->log("Exiting.\n");
  exit;
}

=head2 log_verbose

  Arg[1]      : String $txt - the warning text to log
  Arg[2]      : Int $indent - indentation level for log message
  Example     : my $log = $support->log_filehandle;
                $support->log_verbose('Log this verbose message.\n', 1);
  Description : Logs a message via $self->log if --verbose option was used
  Return type : TRUE on success, FALSE if not verbose
  Exceptions  : none
  Caller      : general

=cut

sub log_verbose {
  my ($self, $txt, $indent) = @_;
  return(0) unless $self->param('verbose');
  $self->log($txt, $indent);
  return(1);
}

=head2 log_stamped

  Arg[1]      : String $txt - the warning text to log
  Arg[2]      : Int $indent - indentation level for log message
  Example     : my $log = $support->log_filehandle;
                $support->log_stamped('Log this stamped message.\n', 1);
  Description : Appends timestamp and memory usage to a message and logs it via
                $self->log
  Return type : TRUE on success
  Exceptions  : none
  Caller      : general

=cut

sub log_stamped {
  my ($self, $txt, $indent) = @_;
  # append timestamp and memory usage to log text
  $txt =~ s/(\n*)$//;
  $txt .= " ".$self->date_and_mem.$1;
  $self->log($txt, $indent);
  return(1);
}

=head2 log_filehandle

  Arg[1]      : (optional) String $mode - file access mode
  Example     : my $log = $support->log_filehandle;
                # print to the filehandle
                print $log 'Lets start logging...\n';
                # log via the wrapper $self->log()
                $support->log('Another log message.\n');
  Description : Returns a filehandle for logging (STDERR by default, logfile if
                set from config or commandline). You can use the filehandle
                directly to print to, or use the smart wrapper $self->log().
                Logging mode (truncate or append) can be set by passing the
                mode as an argument to log_filehandle(), or with the
                --logappend commandline option (default: truncate)
  Return type : Filehandle - the filehandle to log to
  Exceptions  : thrown if logfile can't be opened
  Caller      : general

=cut

sub log_filehandle {
  my ($self, $mode) = @_;
  $mode ||= '>';
  $mode = '>>' if ($self->param('logappend'));
  my $fh = \*STDERR;
  if (my $logfile = $self->param('logfile')) {
    if (my $logpath = $self->param('logpath')) {
      unless (-e $logpath) {
	system("mkdir $logpath") == 0 or
	  $self->log_error("Can't create log dir $logpath: $!\n");
      }
      $logfile = "$logpath/$logfile";
    }
    open($fh, "$mode", $logfile) or throw(
      "Unable to open $logfile for writing: $!");
  }
  $self->{'_log_filehandle'} = $fh;
  return $self->{'_log_filehandle'};
}

=head2 filehandle

  Arg[1]      : String $mode - file access mode
  Arg[2]      : String $file - input or output file
  Example     : my $fh = $support->filehandle('>>', '/path/to/file');
                # print to the filehandle
                print $fh 'Your text goes here...\n';
  Description : Returns a filehandle (*STDOUT for writing, *STDIN for reading
                by default) to print to or read from.
  Return type : Filehandle - the filehandle
  Exceptions  : thrown if file can't be opened
  Caller      : general

=cut

sub filehandle {
  my ($self, $mode, $file) = @_;
  $mode ||= ">";
  my $fh;
  if ($file) {
    open($fh, "$mode", $file) or throw(
      "Unable to open $file for writing: $!");
  } elsif ($mode =~ />/) {
    $fh = \*STDOUT;
  } elsif ($mode =~ /</) {
    $fh = \*STDIN;
  }
  return $fh;
}

=head2 init_log

  Example     : $support->init_log;
  Description : Opens a filehandle to the logfile and prints some header
                information to this file. This includes script name, date, user
                running the script and parameters the script will be running
                with.
  Return type : Filehandle - the log filehandle
  Exceptions  : none
  Caller      : general

=cut

sub init_log {
  my $self = shift;

  # get a log filehandle
  my $log = $self->log_filehandle;

  # print script name, date, user who is running it
  my $hostname = `hostname`;
  chomp $hostname;
  my $script = "$hostname:$Bin/$Script";
  my $user = `whoami`;
  chomp $user;
  $self->log("Script: $script\nDate: ".$self->date."\nUser: $user\n");

  # print parameters the script is running with
  $self->log("Parameters:\n\n");
  $self->log($self->list_all_params);

  # remember start time
  $self->{'_start_time'} = time;

  return $log;
}

=head2 finish_log

  Example     : $support->finish_log;
  Description : Writes footer information to a logfile. This includes the
                number of logged warnings, timestamp and memory footprint.
  Return type : TRUE on success
  Exceptions  : none
  Caller      : general

=cut

sub finish_log {
  my $self = shift;
  $self->log("\nAll done. ".$self->warnings." warnings. ");
  if ($self->{'_start_time'}) {
    $self->log("Runtime ");
    my $diff = time - $self->{'_start_time'};
    my $sec = $diff % 60;
    $diff = ($diff - $sec) / 60;
    my $min = $diff % 60;
    my $hours = ($diff - $min) / 60;
    $self->log("${hours}h ${min}min ${sec}sec ");
  }
  $self->log($self->date_and_mem."\n\n");
  return(1);
}

=head2 date_and_mem

  Example     : print LOG "Time, memory usage: ".$support->date_and_mem."\n";
  Description : Prints a timestamp and the memory usage of your script.
  Return type : String - timestamp and memory usage
  Exceptions  : none
  Caller      : general

=cut

sub date_and_mem {
  my $date = strftime "%Y-%m-%d %T", localtime;
  my $mem = `ps -p $$ -o vsz |tail -1`;
  chomp $mem;
  return "[$date, mem $mem]";
}

=head2 date

  Example     : print "Date: " . $support->date . "\n";
  Description : Prints a nicely formatted timestamp (YYYY-DD-MM hh:mm:ss)
  Return type : String - the timestamp
  Exceptions  : none
  Caller      : general

=cut

sub date {
  return strftime "%Y-%m-%d %T", localtime;
}

=head2 format_time

  Example     : print $support->format_time($gene->modifed_date) . "\n";
  Description : Prints timestamps from the database
  Return type : String - nicely formatted time stamp
  Exceptions  : none
  Caller      : general

=cut


sub date_format {
  my( $self, $time, $format ) = @_;
  my( $d,$m,$y) = (localtime($time))[3,4,5];
  my %S = ('d'=>sprintf('%02d',$d),'m'=>sprintf('%02d',$m+1),'y'=>$y+1900);
  (my $res = $format ) =~s/%(\w)/$S{$1}/ge;
  return $res;
}


=head2 mem

  Example     : print "Memory usage: " . $support->mem . "\n";
  Description : Prints the memory used by your script. Not sure about platform
                dependence of this call ...
  Return type : String - memory usage
  Exceptions  : none
  Caller      : general

=cut

sub mem {
  my $mem = `ps -p $$ -o vsz |tail -1`;
  chomp $mem;
  return $mem;
}

=head2 commify

  Arg[1]      : Int $num - a number to commify
  Example     : print "An easy to read number: ".$self->commify(100000000);
                # will print 100,000,000
  Description : put commas into a number to make it easier to read
  Return type : a string representing the commified number
  Exceptions  : none
  Caller      : general
  Status      : stable

=cut

sub commify {
  my $self = shift;
  my $num = shift;

  $num = reverse($num);
  $num =~ s/(\d\d\d)(?=\d)(?!\d*\.)/$1,/g;

  return scalar reverse $num;
}

=head2 fetch_non_hidden_slices

  Arg[1]      : B::E::SliceAdaptor
  Arg[2]      : B::E::AttributeAdaptor
  Arg[3]      : string $coord_system_name (optional) - 'chromosome' by default
  Arg[4]      : string $coord_system_version (optional) - 'otter' by default
  Example     : $chroms = $support->fetch_non_hidden_slice($sa,$aa);
  Description : retrieve all slices from a loutre database that don't have a hidden attribute
  Return type : arrayref
  Caller      : general
  Status      : stable

=cut

sub fetch_non_hidden_slices {
  my $self = shift;
  my $aa   = shift or throw("You must supply an attribute adaptor");
  my $sa   = shift or throw("You must supply a slice adaptor");
  my $cs   = shift || 'chromosome';
  my $cv   = shift || 'Otter';
  my $visible_chroms;
  foreach my $chrom ( @{$sa->fetch_all($cs,$cv)} ) {
    my $chrom_name = $chrom->name;
    my $attribs = $aa->fetch_all_by_Slice($chrom,'hidden');
    if ( scalar(@$attribs) > 1 ) {
      $self->log_warning("More than one hidden attribute for chromosome $chrom_name\n");
    }
    elsif ($attribs->[0]->value == 0) {				
      push @$visible_chroms, $chrom;
    }
    elsif ($attribs->[0]->value == 1) {	
      $self->log_verbose("chromosome $chrom_name is hidden\n");	
    }
    else {
      $self->log_warning("No hidden attribute for chromosome $chrom_name\n");
    }
  }
  return $visible_chroms;
}

=head2 get_non_hidden_slice_names

  Arg[1]      : B::E::SliceAdaptor
  Arg[2]      : B::E::AttributeAdaptor
  Arg[3]      : string $coord_system_name (optional) - 'chromosome' by default
  Arg[4]      : string $coord_system_version (optional) - 'otter' by default
  Example     : $chrom_names = $support->get_non_hidden_slice_names($sa,$aa);
  Description : retrieve names of all slices from a loutre database that don't have a hidden attribute
  Return type : arrayref of names of all non-hidden slices
  Caller      : general
  Status      : stable

=cut

sub get_non_hidden_slice_names {
  my $self = shift;
  my $aa   = shift or throw("You must supply an attribute adaptor");
  my $sa   = shift or throw("You must supply a slice adaptor");
  my $cs   = shift || 'chromosome';
  my $cv   = shift || 'Otter';
  my $visible_chrom_names;
  foreach my $chrom ( @{$sa->fetch_all($cs,$cv)} ) {
    my $chrom_name = $chrom->seq_region_name;
    my $attribs = $aa->fetch_all_by_Slice($chrom,'hidden');
    if ( scalar(@$attribs) > 1 ) {
      $self->log_warning("More than one hidden attribute for chromosome $chrom_name\n");
    }
    elsif ($attribs->[0]->value == 0) {				
      push @$visible_chrom_names, $chrom_name;
    }
    elsif ($attribs->[0]->value == 1) {	
      $self->log_verbose("chromosome $chrom_name is hidden\n");	
    }
    else {
      $self->log_warning("No hidden attribute for chromosome $chrom_name\n");
    }
  }
  return $visible_chrom_names;
}


=head2 get_wanted_chromosomes

  Arg[1]      : B::E::U::ConversionSupport
  Arg[2]      : B::E::SliceAdaptor
  Arg[3]      : B::E::AttributeAdaptor
  Arg[4]      : string $coord_system_name (optional) - 'chromosome' by default
  Arg[5]      : string $coord_system_version (optional) - 'otter' by default
  Example     : $chr_names = $support->get_wanted_chromosomes($laa,$lsa);
  Description : retrieve names of slices from a lutra database that are ready for dumping to Vega.
                Deals with list of names to ignore (ignore_chr = LIST)
  Return type : arrayref of slices
  Caller      : general
  Status      : stable

=cut

sub get_wanted_chromosomes {
  my $self = shift;
  my $aa   = shift or throw("You must supply an attribute adaptor");
  my $sa   = shift or throw("You must supply a slice adaptor");
  my $cs   = shift || 'chromosome';
  my $cv   = shift || 'Otter';
  my $export_mode = $self->param('release_type');
  my $release = $self->param('vega_release');
  my $names;
  my $chroms  = $self->fetch_non_hidden_slices($aa,$sa,$cs,$cv);
 CHROM:
  foreach my $chrom (@$chroms) {
    my $attribs = $aa->fetch_all_by_Slice($chrom);
    my $vals = $self->get_attrib_values($attribs,'vega_export_mod');
    if (scalar(@$vals > 1)) {
      $self->log_warning ("Multiple attribs for \'vega_export_mod\', please fix before continuing");
      exit;
    }
    next CHROM if (! grep { $_ eq $export_mode} @$vals);
    $vals =  $self->get_attrib_values($attribs,'vega_release',$release);	
    if (scalar(@$vals > 1)) {
      $self->log_warning ("Multiple attribs for \'vega_release\' value = $release , please fix before continuing");
      exit;
    }
    next CHROM if (! grep { $_ eq $release} @$vals);
    my $name = $chrom->seq_region_name;
    if (my @ignored = $self->param('ignore_chr')) {
      next CHROM if (grep {$_ eq $name} @ignored);
    }
    push @{$names}, $name;
  }
  return $names;
}


=head2 get_attrib_values

  Arg[1]      : Arrayref of B::E::Attributes
  Arg[2]      : 'code' to search for
  Arg[3]      : 'value' to search for (optional)
  Example     : my $c = $self->get_attrib_values($attribs,'name'));
  Description : (i) In the absence of an attribute value argument, examines an arrayref
                of B::E::Attributes for a particular attribute type, returning the values
                for each attribute of that type. Can therefore be used to test for the
                number of attributes of that type.
                (ii) In the presence of the optional value argument it returns all
                attributes with that value ie can be used to test for the presence of an
                attribute with that particular value.
  Return type : arrayref of values for that attribute
  Caller      : general
  Status      : stable

=cut

sub get_attrib_values {
  my $self    = shift;
  my $attribs = shift;
  my $code    = shift;
  my $value   = shift;
  if (my @atts = grep {$_->code eq $code } @$attribs) {
    my $r = [];
    if ($value) {
      if (my @values = grep {$_->value eq $value} @atts) {
	foreach (@values) {
	  push @$r, $_->value;
	}
	return $r;
      }
      else {
	return [];
      }
    }
    else {
      foreach (@atts) {
	push @$r, $_->value;
      }
      return $r;
    }
  }
  else {
    return [];
  }
}

=head2 fix_attrib_value

  Arg[1]      : Arrayref of existing B::E::Attributes
  Arg[2]      : dbID of object
  Arg[3]      : name of object (just for reporting)
  Arg[4]      : attrib_type.code
  Arg[5]      : attrib_type.value
  Arg[6]      : interactive ? (0 by default)
  Arg[7]      : table
  Example     : $support->fix_attrib_value($attribs,$chr_id,$chr_name,'vega_export_mod','N',1);
  Description : adds a new attribute to an object, or updates an existing attribute with a new value
                Can be run in interactive or non-interactive mode (default)
  Return type : arrayref of results
  Caller      : general
  Status      : only ever tested with seq_region_attributes to date

=cut

sub fix_attrib_value {
  my $self        = shift;
  my $attribs     = shift;
  my $id          = shift;
  my $name        = shift;
  my $code        = shift;
  my $value       = shift;
  my $interact    = shift || 0;
  my $table       = shift || 'seq_region_attrib';

  #transiently set interactive parameter to zero
  my $int_before;
  if (! $interact) {
    $int_before = $self->param('interactive');
    $self->param('interactive',0);
  }

  #get any existing value(s) for this attribute
  my $existings = $self->get_attrib_values($attribs,$code);
	
  #add a new attribute if there is none...
  if (! @$existings ) {
    if ($self->user_proceed("Do you want to set $name attrib (code = $code) to value $value ?")) {
      my $r = $self->store_new_attribute($id,$code,$value);

      #reset interactive parameter
      $self->param('interactive',$int_before) if (! $interact);
      return $r;
		}
  }
  #...warn and exit if you're trying to update more than one value for the same attribute...
  elsif (scalar @$existings > 1) {
    $self->log_warning("You shouldn't be trying to update multiple attributes with the same code at once ($name:$code,$value), looks like you have duplicate entries in the (seq_region_)attrib table\n");
    exit;
  }

  #...or update an attribute with new values...
  else {
    my $existing = $existings->[0];
    if ($existing ne $value) {
      if ($self->user_proceed("Do you want to reset $name attrib (code = $code) from $existing to $value ?")) {
	my $r = $self->update_attribute($id,$code,$value);
	$self->param('interactive',$int_before) if (! $interact);
	push @$r, $existing;
	return $r;
      }
    }
    #...or make no change
    else {
      $self->param('interactive',$int_before) if (! $interact);
      return [];
    }
  }
}

=head2 _get_attrib_id

  Arg[1]      : attrib_type.code 
  Arg[2]      : database handle 
  Example     : $self->_get_attrib_id('name',$dbh)
  Description : get attrib_type.attrib_type_id from a attrib_type.code
  Return type : attrib_type.attrib_type_id 
  Caller      : internal
  Status      : stable

=cut

sub _get_attrib_id {
  my $self        = shift;
  my $attrib_code = shift;
  my $dbh         = shift;
  my ($attrib_id) = $dbh->selectrow_array(
    qq(select attrib_type_id
           from attrib_type
           where code = ?),
    {},
    ($attrib_code)
  );
  if (! $attrib_id) {
    $self->log_warning("There is no attrib_type_id for code $attrib_code, please patch the attrib_table\n");
    exit;
  }
  else {
    return $attrib_id;
  }
}

=head2 store_new_attribute

  Arg[1]      : seq_region.seq_region_id
  Arg[2]      : attrib_type.code
  Arg[3]      : attrib_type.value
  ARG[4]      : table to update (seq_region_attribute by default)
  Example     : $support->store_new_attribute(23,name,5);
  Description : uses MySQL to store an entry (code and value) in an attribute table 
                (seq_region_attrib by default)
  Return type : array_ref
  Caller      : general
  Status      : stable

=cut

sub store_new_attribute {
  my $self         = shift;
  my $sr_id        = shift;
  my $attrib_code  = shift;
  my $attrib_value = shift || '';
  my $table        = shift || 'seq_region_attrib';

  #get database handle
  my $dbh = $self->get_dbconnection('loutre');
  #get attrib_type_id for this particular attribute
  my $attrib_id = $self->_get_attrib_id($attrib_code,$dbh);
  #store
  my $r = $dbh->do(
    qq(insert into $table
           values (?,?,?)),
    {},
    ($sr_id,$attrib_id,$attrib_value)
  );
  return ['Stored',$r];
}

=head2 update_attribute

  Arg[1]      : seq_region.seq_region_id
  Arg[2]      : attrib_type.code
  Arg[3]      : attrib_type.value
  ARG[4]      : table to update (seq_region_attribute by default)
  Example     : $support->update_attribute(23,name,5);
  Description : uses MySQL to update an attribute table (seq_region_attrib by default)
  Return type : array_ref
  Caller      : general
  Status      : stable

=cut

sub update_attribute {
  my $self = shift;
  my $sr_id = shift;
  my $attrib_code  = shift;
  my $attrib_value = shift;
  my $table        = shift || 'seq_region_attrib';
  my $dbh = $self->get_dbconnection('loutre');
  my $attrib_id = $self->_get_attrib_id($attrib_code,$dbh);
  #update
  my $r = $dbh->do(
    qq(update $table
           set value = ?
           where seq_region_id = $sr_id
           and attrib_type_id = $attrib_id),
    {},
    ($attrib_value)
  );
  return ['Updated',$r];
}

1;