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}
=head2 get_species_scientific_name
Arg[1] : Bio::EnsEMBL::DBSQL::DBAdaptor $dba
Example : my $species = $support->get_species_scientific_name($dba);
Description : Retrieves the species scientific name (Genus species) from the
meta table
Return type : String - species scientific name
Exceptions : thrown if species name can not be determined from db
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Caller : general
=cut
sub get_species_scientific_name {
my ($self, $dba) = @_;
my $sql_tmp = "SELECT meta_value FROM meta WHERE meta_key = \'species.classification\' ORDER BY meta_id";
my $sql = $dba->dbc->add_limit_clause($sql_tmp,2);
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my $sth = $dba->dbc->db_handle->prepare($sql);
$sth->execute;
my @sp;
while (my @row = $sth->fetchrow_array) {
push @sp, $row[0];
}
$sth->finish;
my $species = join(" ", reverse @sp);
$self->throw("Could not determine species scientific name from database.")
unless $species;
return $species;
}
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=head2 species
Arg[1] : (optional) String $species - species name to set
Example : my $species = $support->species;
my $url = "http://vega.sanger.ac.uk/$species/";
Description : Getter/setter for species name (Genus_species). If not set, it's
determined from database's meta table
Return type : String - species name
Exceptions : none
Caller : general
=cut
sub species {
my $self = shift;
$self->{'_species'} = shift if (@_);
# get species name from database if not set
unless ($self->{'_species'}) {
$self->{'_species'} = join('_',
split(/ /, $self->get_species_scientific_name));
}
return $self->{'_species'};
}
=head2 sort_chromosomes
Arg[1] : (optional) Hashref $chr_hashref - Hashref with chr_name as keys
Example : my $chr = { '6-COX' => 1, '1' => 1, 'X' => 1 };
my @sorted = $support->sort_chromosomes($chr);
Description : Sorts chromosomes in an intuitive way (numerically, then
alphabetically). If no chromosome hashref is passed, it's
retrieve by calling $self->get_chrlength()
Return type : List - sorted chromosome names
Exceptions : thrown if no hashref is provided
Caller : general
=cut
sub sort_chromosomes {
my ($self, $chr_hashref) = @_;
$chr_hashref = $self->get_chrlength unless ($chr_hashref);
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throw("You have to pass a hashref of your chromosomes")
unless ($chr_hashref and ref($chr_hashref) eq 'HASH');
return (sort _by_chr_num keys %$chr_hashref);
}
=head2 _by_chr_num
Example : my @sorted = sort _by_chr_num qw(X, 6-COX, 14, 7);
Description : Subroutine to use in sort for sorting chromosomes. Sorts
numerically, then alphabetically
Return type : values to be used by sort
Exceptions : none
Caller : internal ($self->sort_chromosomes)
=cut
sub _by_chr_num {
my @awords = split /-/, $a;
my @bwords = split /-/, $b;
my $anum = $awords[0];
my $bnum = $bwords[0];
if ($anum !~ /^[0-9]*$/) {
if ($bnum !~ /^[0-9]*$/) {
return $anum cmp $bnum;
} else {
return 1;
}
}
if ($bnum !~ /^[0-9]*$/) {
return -1;
}
if ($anum <=> $bnum) {
return $anum <=> $bnum;
} else {
if ($#awords == 0) {
return -1;
} elsif ($#bwords == 0) {
return 1;
} else {
return $awords[1] cmp $bwords[1];
}
}
}
=head2 split_chromosomes_by_size
Arg[1] : (optional) Int $cutoff - the cutoff in bp between small and
large chromosomes
Arg[2] : (optional) Boolean to include duplicate regions, ie PAR or not
(default is no)
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Example : my $chr_slices = $support->split_chromosomes_by_size;
foreach my $block_size (keys %{ $chr_slices }) {
print "Chromosomes with blocksize $block_size: ";
print join(", ", map { $_->seq_region_name }
@{ $chr_slices->{$block_size} });
}
Description : Determines block sizes for storing DensityFeatures on
chromosomes, and return slices for each chromosome. The block
size is determined so that you have 150 bins for the smallest
chromosome over 5 Mb in length. For chromosomes smaller than 5
Mb, an additional smaller block size is used to yield 150 bins
for the overall smallest chromosome. This will result in
reasonable resolution for small chromosomes and high
performance for big ones.
Return type : Hashref (key: block size; value: Arrayref of chromosome
Bio::EnsEMBL::Slices)
Exceptions : none
Caller : density scripts
=cut
sub split_chromosomes_by_size {
my $self = shift;
my $cutoff = shift || 5000000;
my $dup = shift || 0;
my $slice_adaptor = $self->dba->get_SliceAdaptor;
my $top_slices;
if ($self->param('chromosomes')) {
foreach my $chr ($self->param('chromosomes')) {
push @{ $top_slices }, $slice_adaptor->fetch_by_region('chromosome', $chr);
} else {
$top_slices = $slice_adaptor->fetch_all('chromosome',undef,0,$dup);
}
my ($big_chr, $small_chr, $min_big_chr, $min_small_chr);
foreach my $slice (@{ $top_slices }) {
next if ($slice->length eq 10000); #hack for chrY pseudoslice
if ($slice->length < $cutoff) {
if (! $min_small_chr or ($min_small_chr > $slice->length)) {
$min_small_chr = $slice->length;
}
# push small chromosomes onto $small_chr
push @{ $small_chr }, $slice;
if (! $min_big_chr or ($min_big_chr > $slice->length) && $slice->length > $cutoff) {
$min_big_chr = $slice->length;
}
# push _all_ chromosomes onto $big_chr
push @{ $big_chr }, $slice;
}
my $chr_slices;
$chr_slices->{int($min_big_chr/150)} = $big_chr if $min_big_chr;
$chr_slices->{int($min_small_chr/150)} = $small_chr if $min_small_chr;
=head2 log
Arg[1] : String $txt - the text to log
Arg[2] : Int $indent - indentation level for log message
Example : my $log = $support->log_filehandle;
$support->log('Log foo.\n', 1);
Description : Logs a message to the filehandle initialised by calling
$self->log_filehandle(). You can supply an indentation level
to get nice hierarchical log messages.
Return type : true on success
Exceptions : thrown when no filehandle can be obtained
Caller : general
=cut
sub log {
my ($self, $txt, $indent) = @_;
$indent ||= 0;
# strip off leading linebreaks so that indenting doesn't break
$txt =~ s/^(\n*)//;
$txt = $1." "x$indent . $txt;
my $fh = $self->{'_log_filehandle'};
throw("Unable to obtain log filehandle") unless $fh;
print $fh "$txt";
return(1);
}
=head2 log_warning
Arg[1] : String $txt - the warning text to log
Arg[2] : Int $indent - indentation level for log message
Arg[3] : Bool - add a line break before warning if true
Example : my $log = $support->log_filehandle;
$support->log_warning('Log foo.\n', 1);
Description : Logs a message via $self->log and increases the warning counter.
Return type : true on success
Exceptions : none
Caller : general
=cut
sub log_warning {
my ($self, $txt, $indent, $break) = @_;
$txt = "WARNING: " . $txt;
$txt = "\n$txt" if ($break);
$self->log($txt, $indent);
$self->{'_warnings'}++;
return(1);
}
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=head2 log_error
Arg[1] : String $txt - the error text to log
Arg[2] : Int $indent - indentation level for log message
Example : my $log = $support->log_filehandle;
$support->log_error('Log foo.\n', 1);
Description : Logs a message via $self->log and exits the script.
Return type : none
Exceptions : none
Caller : general
=cut
sub log_error {
my ($self, $txt, $indent) = @_;
$txt = "ERROR: ".$txt;
$self->log($txt, $indent);
$self->log("Exiting.\n");
exit;
}
=head2 log_verbose
Arg[1] : String $txt - the warning text to log
Arg[2] : Int $indent - indentation level for log message
Example : my $log = $support->log_filehandle;
$support->log_verbose('Log this verbose message.\n', 1);
Description : Logs a message via $self->log if --verbose option was used
Return type : TRUE on success, FALSE if not verbose
Exceptions : none
Caller : general
=cut
sub log_verbose {
my ($self, $txt, $indent) = @_;
return(0) unless $self->param('verbose');
$self->log($txt, $indent);
return(1);
}
=head2 log_stamped
Arg[1] : String $txt - the warning text to log
Arg[2] : Int $indent - indentation level for log message
Example : my $log = $support->log_filehandle;
$support->log_stamped('Log this stamped message.\n', 1);
Description : Appends timestamp and memory usage to a message and logs it via
$self->log
Return type : TRUE on success
Exceptions : none
Caller : general
=cut
sub log_stamped {
my ($self, $txt, $indent) = @_;
# append timestamp and memory usage to log text
$txt =~ s/(\n*)$//;
$txt .= " ".$self->date_and_mem.$1;
$self->log($txt, $indent);
return(1);
}
=head2 log_filehandle
Arg[1] : (optional) String $mode - file access mode
Example : my $log = $support->log_filehandle;
# print to the filehandle
print $log 'Lets start logging...\n';
# log via the wrapper $self->log()
$support->log('Another log message.\n');
Description : Returns a filehandle for logging (STDERR by default, logfile if
set from config or commandline). You can use the filehandle
directly to print to, or use the smart wrapper $self->log().
Logging mode (truncate or append) can be set by passing the
mode as an argument to log_filehandle(), or with the
--logappend commandline option (default: truncate)
Return type : Filehandle - the filehandle to log to
Exceptions : thrown if logfile can't be opened
Caller : general
=cut
sub log_filehandle {
my ($self, $mode) = @_;
$mode ||= '>';
$mode = '>>' if ($self->param('logappend'));
my $fh = \*STDERR;
if (my $logfile = $self->param('logfile')) {
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if (my $logpath = $self->param('logpath')) {
unless (-e $logpath) {
system("mkdir $logpath") == 0 or
$self->log_error("Can't create log dir $logpath: $!\n");
}
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$logfile = "$logpath/$logfile";
}
open($fh, "$mode", $logfile) or throw(
"Unable to open $logfile for writing: $!");
}
$self->{'_log_filehandle'} = $fh;
return $self->{'_log_filehandle'};
}
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=head2 filehandle
Arg[1] : String $mode - file access mode
Arg[2] : String $file - input or output file
Example : my $fh = $support->filehandle('>>', '/path/to/file');
# print to the filehandle
print $fh 'Your text goes here...\n';
Description : Returns a filehandle (*STDOUT for writing, *STDIN for reading
by default) to print to or read from.
Return type : Filehandle - the filehandle
Exceptions : thrown if file can't be opened
Caller : general
=cut
sub filehandle {
my ($self, $mode, $file) = @_;
$mode ||= ">";
my $fh;
if ($file) {
open($fh, "$mode", $file) or throw(
"Unable to open $file for writing: $!");
} elsif ($mode =~ />/) {
$fh = \*STDOUT;
} elsif ($mode =~ /</) {
$fh = \*STDIN;
}
return $fh;
}
=head2 init_log
Example : $support->init_log;
Description : Opens a filehandle to the logfile and prints some header
information to this file. This includes script name, date, user
running the script and parameters the script will be running
with.
Return type : Filehandle - the log filehandle
Exceptions : none
Caller : general
=cut
sub init_log {
my $self = shift;
# get a log filehandle
my $log = $self->log_filehandle;
# print script name, date, user who is running it
my $hostname = `hostname`;
chomp $hostname;
my $script = "$hostname:$Bin/$Script";
my $user = `whoami`;
chomp $user;
$self->log("Script: $script\nDate: ".$self->date."\nUser: $user\n");
# print parameters the script is running with
$self->log("Parameters:\n\n");
$self->log($self->list_all_params);
# remember start time
$self->{'_start_time'} = time;
return $log;
}
=head2 finish_log
Example : $support->finish_log;
Description : Writes footer information to a logfile. This includes the
number of logged warnings, timestamp and memory footprint.
Return type : TRUE on success
Exceptions : none
Caller : general
=cut
sub finish_log {
my $self = shift;
$self->log("\nAll done. ".$self->warnings." warnings. ");
if ($self->{'_start_time'}) {
$self->log("Runtime ");
my $diff = time - $self->{'_start_time'};
my $sec = $diff % 60;
$diff = ($diff - $sec) / 60;
my $min = $diff % 60;
my $hours = ($diff - $min) / 60;
$self->log("${hours}h ${min}min ${sec}sec ");
}
$self->log($self->date_and_mem."\n\n");
return(1);
}
=head2 date_and_mem
Example : print LOG "Time, memory usage: ".$support->date_and_mem."\n";
Description : Prints a timestamp and the memory usage of your script.
Return type : String - timestamp and memory usage
Exceptions : none
Caller : general
=cut
sub date_and_mem {
my $date = strftime "%Y-%m-%d %T", localtime;
chomp $mem;
return "[$date, mem $mem]";
}
=head2 date
Example : print "Date: " . $support->date . "\n";
Description : Prints a nicely formatted timestamp (YYYY-DD-MM hh:mm:ss)
Return type : String - the timestamp
Exceptions : none
Caller : general
=cut
sub date {
return strftime "%Y-%m-%d %T", localtime;
}
=head2 format_time
Example : print $support->format_time($gene->modifed_date) . "\n";
Description : Prints timestamps from the database
Return type : String - nicely formatted time stamp
Exceptions : none
Caller : general
=cut
sub date_format {
my( $self, $time, $format ) = @_;
my( $d,$m,$y) = (localtime($time))[3,4,5];
my %S = ('d'=>sprintf('%02d',$d),'m'=>sprintf('%02d',$m+1),'y'=>$y+1900);
(my $res = $format ) =~s/%(\w)/$S{$1}/ge;
return $res;
}
=head2 mem
Example : print "Memory usage: " . $support->mem . "\n";
Description : Prints the memory used by your script. Not sure about platform
dependence of this call ...
Return type : String - memory usage
Exceptions : none
Caller : general
=cut
sub mem {
chomp $mem;
return $mem;
}
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=head2 commify
Arg[1] : Int $num - a number to commify
Example : print "An easy to read number: ".$self->commify(100000000);
# will print 100,000,000
Description : put commas into a number to make it easier to read
Return type : a string representing the commified number
Exceptions : none
Caller : general
Status : stable
=cut
sub commify {
my $self = shift;
my $num = shift;
$num = reverse($num);
$num =~ s/(\d\d\d)(?=\d)(?!\d*\.)/$1,/g;
return scalar reverse $num;
}
=head2 fetch_non_hidden_slices
Arg[1] : B::E::SliceAdaptor
Arg[2] : B::E::AttributeAdaptor
Arg[3] : string $coord_system_name (optional) - 'chromosome' by default
Arg[4] : string $coord_system_version (optional) - 'otter' by default
Example : $chroms = $support->fetch_non_hidden_slice($sa,$aa);
Description : retrieve all slices from a loutre database that don't have a hidden attribute
Return type : arrayref
Caller : general
Status : stable
=cut
sub fetch_non_hidden_slices {
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my $self = shift;
my $aa = shift or throw("You must supply an attribute adaptor");
my $sa = shift or throw("You must supply a slice adaptor");
my $cs = shift || 'chromosome';
my $cv = shift || 'Otter';
my $visible_chroms;
foreach my $chrom ( @{$sa->fetch_all($cs,$cv)} ) {
my $chrom_name = $chrom->name;
my $attribs = $aa->fetch_all_by_Slice($chrom,'hidden');
if ( scalar(@$attribs) > 1 ) {
$self->log_warning("More than one hidden attribute for chromosome $chrom_name\n");
}
elsif ($attribs->[0]->value == 0) {
push @$visible_chroms, $chrom;
}
elsif ($attribs->[0]->value == 1) {
$self->log_verbose("chromosome $chrom_name is hidden\n");
}
else {
$self->log_warning("No hidden attribute for chromosome $chrom_name\n");
}
}
return $visible_chroms;
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=head2 get_non_hidden_slice_names
Arg[1] : B::E::SliceAdaptor
Arg[2] : B::E::AttributeAdaptor
Arg[3] : string $coord_system_name (optional) - 'chromosome' by default
Arg[4] : string $coord_system_version (optional) - 'otter' by default
Example : $chrom_names = $support->get_non_hidden_slice_names($sa,$aa);
Description : retrieve names of all slices from a loutre database that don't have a hidden attribute
Return type : arrayref of names of all non-hidden slices
Caller : general
Status : stable
=cut
sub get_non_hidden_slice_names {
my $self = shift;
my $aa = shift or throw("You must supply an attribute adaptor");
my $sa = shift or throw("You must supply a slice adaptor");
my $cs = shift || 'chromosome';
my $cv = shift || 'Otter';
my $visible_chrom_names;
foreach my $chrom ( @{$sa->fetch_all($cs,$cv)} ) {
my $chrom_name = $chrom->seq_region_name;
my $attribs = $aa->fetch_all_by_Slice($chrom,'hidden');
if ( scalar(@$attribs) > 1 ) {
$self->log_warning("More than one hidden attribute for chromosome $chrom_name\n");
}
elsif ($attribs->[0]->value == 0) {
push @$visible_chrom_names, $chrom_name;
}
elsif ($attribs->[0]->value == 1) {
$self->log_verbose("chromosome $chrom_name is hidden\n");
}
else {
$self->log_warning("No hidden attribute for chromosome $chrom_name\n");
}
}
return $visible_chrom_names;
}
=head2 get_wanted_chromosomes
Arg[1] : B::E::U::ConversionSupport
Arg[2] : B::E::SliceAdaptor
Arg[3] : B::E::AttributeAdaptor
Arg[4] : string $coord_system_name (optional) - 'chromosome' by default
Arg[5] : string $coord_system_version (optional) - 'otter' by default
Example : $chr_names = $support->get_wanted_chromosomes($laa,$lsa);
Description : retrieve names of slices from a lutra database that are ready for dumping to Vega.
Deals with list of names to ignore (ignore_chr = LIST)
Caller : general
Status : stable
=cut
sub get_wanted_chromosomes {
my $self = shift;
my $aa = shift or throw("You must supply an attribute adaptor");
my $sa = shift or throw("You must supply a slice adaptor");
my $cs = shift || 'chromosome';
my $cv = shift || 'Otter';
my $export_mode = $self->param('release_type');
my $release = $self->param('vega_release');
my $names;
my $chroms = $self->fetch_non_hidden_slices($aa,$sa,$cs,$cv);
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foreach my $chrom (@$chroms) {
my $attribs = $aa->fetch_all_by_Slice($chrom);
my $vals = $self->get_attrib_values($attribs,'vega_export_mod');
if (scalar(@$vals > 1)) {
$self->log_warning ("Multiple attribs for \'vega_export_mod\', please fix before continuing");
exit;
}
next CHROM if (! grep { $_ eq $export_mode} @$vals);
$vals = $self->get_attrib_values($attribs,'vega_release',$release);
if (scalar(@$vals > 1)) {
$self->log_warning ("Multiple attribs for \'vega_release\' value = $release , please fix before continuing");
exit;
}
next CHROM if (! grep { $_ eq $release} @$vals);
my $name = $chrom->seq_region_name;
if (my @ignored = $self->param('ignore_chr')) {
next CHROM if (grep {$_ eq $name} @ignored);
}
push @{$names}, $name;
}
return $names;
=head2 get_attrib_values
Arg[1] : Arrayref of B::E::Attributes
Arg[2] : 'code' to search for
Arg[3] : 'value' to search for (optional)
Example : my $c = $self->get_attrib_values($attribs,'name'));
Description : (i) In the absence of an attribute value argument, examines an arrayref
of B::E::Attributes for a particular attribute type, returning the values
for each attribute of that type. Can therefore be used to test for the
number of attributes of that type.
(ii) In the presence of the optional value argument it returns all
attributes with that value ie can be used to test for the presence of an
attribute with that particular value.
Return type : arrayref of values for that attribute
Caller : general
Status : stable
=cut
sub get_attrib_values {
my $self = shift;
my $attribs = shift;
my $code = shift;
my $value = shift;
if (my @atts = grep {$_->code eq $code } @$attribs) {
my $r = [];
if ($value) {
if (my @values = grep {$_->value eq $value} @atts) {
foreach (@values) {
push @$r, $_->value;
return $r;
}
else {
return [];
}
}
else {
foreach (@atts) {
push @$r, $_->value;
}
return $r;
}
}
else {
return [];
}
Arg[1] : Arrayref of existing B::E::Attributes
Arg[2] : dbID of object
Arg[3] : name of object (just for reporting)
Arg[4] : attrib_type.code
Arg[5] : attrib_type.value
Arg[6] : interactive ? (0 by default)
Arg[7] : table
Example : $support->fix_attrib_value($attribs,$chr_id,$chr_name,'vega_export_mod','N',1);
Description : adds a new attribute to an object, or updates an existing attribute with a new value
Can be run in interactive or non-interactive mode (default)
Caller : general
Status : only ever tested with seq_region_attributes to date
=cut
sub fix_attrib_value {
my $self = shift;
my $attribs = shift;
my $id = shift;
my $name = shift;
my $code = shift;
my $value = shift;
my $interact = shift || 0;
my $table = shift || 'seq_region_attrib';
#transiently set interactive parameter to zero
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my $int_before;
if (! $interact) {
$int_before = $self->param('interactive');
$self->param('interactive',0);
}
#get any existing value(s) for this attribute
my $existings = $self->get_attrib_values($attribs,$code);
#add a new attribute if there is none...
if (! @$existings ) {
if ($self->user_proceed("Do you want to set $name attrib (code = $code) to value $value ?")) {
my $r = $self->store_new_attribute($id,$code,$value);
#reset interactive parameter
$self->param('interactive',$int_before) if (! $interact);
return $r;
}
}
#...warn and exit if you're trying to update more than one value for the same attribute...
elsif (scalar @$existings > 1) {
$self->log_warning("You shouldn't be trying to update multiple attributes with the same code at once ($name:$code,$value), looks like you have duplicate entries in the (seq_region_)attrib table\n");
exit;
}
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else {
my $existing = $existings->[0];
if ($existing ne $value) {
if ($self->user_proceed("Do you want to reset $name attrib (code = $code) from $existing to $value ?")) {
my $r = $self->update_attribute($id,$code,$value);
$self->param('interactive',$int_before) if (! $interact);
push @$r, $existing;
return $r;
}
}
#...or make no change
else {
$self->param('interactive',$int_before) if (! $interact);
return [];
}
}
}
=head2 _get_attrib_id
Arg[1] : attrib_type.code
Arg[2] : database handle
Example : $self->_get_attrib_id('name',$dbh)
Description : get attrib_type.attrib_type_id from a attrib_type.code
Return type : attrib_type.attrib_type_id
Caller : internal
Status : stable
=cut
sub _get_attrib_id {
my $self = shift;
my $attrib_code = shift;
my $dbh = shift;
my ($attrib_id) = $dbh->selectrow_array(
qq(select attrib_type_id
from attrib_type
where code = ?),
{},
($attrib_code)
);
if (! $attrib_id) {
$self->log_warning("There is no attrib_type_id for code $attrib_code, please patch the attrib_table\n");
exit;
}
else {
return $attrib_id;
}
}
=head2 store_new_attribute
Arg[1] : seq_region.seq_region_id
Arg[2] : attrib_type.code
Arg[3] : attrib_type.value
ARG[4] : table to update (seq_region_attribute by default)
Example : $support->store_new_attribute(23,name,5);
Description : uses MySQL to store an entry (code and value) in an attribute table
(seq_region_attrib by default)
Return type : array_ref
Caller : general
Status : stable
=cut
sub store_new_attribute {
my $self = shift;
my $sr_id = shift;
my $attrib_code = shift;
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my $table = shift || 'seq_region_attrib';
#get database handle
my $dbh = $self->get_dbconnection('loutre');
#get attrib_type_id for this particular attribute
my $attrib_id = $self->_get_attrib_id($attrib_code,$dbh);
#store
my $r = $dbh->do(
qq(insert into $table
values (?,?,?)),
{},
($sr_id,$attrib_id,$attrib_value)
);
return ['Stored',$r];
}
=head2 update_attribute
Arg[1] : seq_region.seq_region_id
Arg[2] : attrib_type.code
Arg[3] : attrib_type.value
ARG[4] : table to update (seq_region_attribute by default)
Example : $support->update_attribute(23,name,5);
Description : uses MySQL to update an attribute table (seq_region_attrib by default)
Return type : array_ref
Caller : general
Status : stable
=cut
sub update_attribute {
my $self = shift;
my $sr_id = shift;
my $attrib_code = shift;
my $attrib_value = shift;
my $table = shift || 'seq_region_attrib';
my $dbh = $self->get_dbconnection('loutre');
my $attrib_id = $self->_get_attrib_id($attrib_code,$dbh);
#update
my $r = $dbh->do(
qq(update $table
set value = ?
where seq_region_id = $sr_id
and attrib_type_id = $attrib_id),
{},
($attrib_value)
);
return ['Updated',$r];
}