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ensembl-gh-mirror
ensembl
Commits
bc3074db
Commit
bc3074db
authored
22 years ago
by
Arne Stabenau
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added supporting feature test
parent
3841d1bd
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Changes
1
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1 changed file
modules/t/gene.t
+134
-22
134 additions, 22 deletions
modules/t/gene.t
with
134 additions
and
22 deletions
modules/t/gene.t
+
134
−
22
View file @
bc3074db
...
...
@@ -4,7 +4,7 @@ use warnings;
BEGIN
{
$|
=
1
;
use
Test
;
plan
tests
=>
2
2
;
plan
tests
=>
2
6
;
}
use
MultiTestDB
;
...
...
@@ -13,7 +13,8 @@ use TestUtils qw ( debug );
use
Bio::EnsEMBL::
Gene
;
# switch on the debug prints
my
$verbose
=
0
;
our
$verbose
=
0
;
debug
(
"
Startup test
"
);
ok
(
1
);
...
...
@@ -58,11 +59,16 @@ ok( scalar @$links == 6 );
# now create a new gene ...
my
$slice
=
$db
->
get_SliceAdaptor
()
->
fetch_by_chr_start_end
(
"
20
",
30264615
,
30265615
);
my
$sa
=
$db
->
get_SliceAdaptor
();
my
$slice
=
$sa
->
fetch_by_chr_start_end
(
"
20
",
30_249_935
,
31_254_640
);
debug
(
"
Slice from SliceAdaptor
"
);
ok
(
$slice
);
my
$analysis
=
$db
->
get_AnalysisAdaptor
->
fetch_by_logic_name
("
ensembl
");
my
$f_analysis
=
$db
->
get_AnalysisAdaptor
->
fetch_by_logic_name
("
Vertrna
");
debug
(
"
Analysis from AnalysisAdaptor
"
);
ok
(
$analysis
);
...
...
@@ -82,26 +88,106 @@ my $translation2 = Bio::EnsEMBL::Translation->new();
ok
(
$gene
);
$ex1
->
start
(
5
);
$ex1
->
end
(
10
);
$ex1
->
start
(
13586
);
$ex1
->
end
(
13735
);
$ex1
->
phase
(
0
);
$ex1
->
end_phase
(
0
);
$ex1
->
contig
(
$slice
);
$ex1
->
strand
(
1
);
$ex1
->
analysis
(
$analysis
);
$ex2
->
start
(
15
);
$ex2
->
end
(
23
);
my
@feats
;
my
$fp
=
new
Bio::EnsEMBL::
FeaturePair
;
$fp
->
start
(
13586
);
$fp
->
end
(
13705
);
$fp
->
strand
(
1
);
$fp
->
score
(
10
);
$fp
->
contig
(
$slice
);
$fp
->
hstart
(
100
);
$fp
->
hend
(
219
);
$fp
->
hstrand
(
1
);
$fp
->
hseqname
('
dummy-hid
');
push
(
@feats
,
$fp
);
$fp
=
new
Bio::EnsEMBL::
FeaturePair
;
$fp
->
start
(
13707
);
$fp
->
end
(
13735
);
$fp
->
strand
(
1
);
$fp
->
score
(
10
);
$fp
->
contig
(
$slice
);
$fp
->
seqname
(
1
);
$fp
->
hstart
(
220
);
$fp
->
hend
(
248
);
$fp
->
hstrand
(
1
);
$fp
->
hseqname
('
dummy-hid
');
push
(
@feats
,
$fp
);
#
#
# 2 Test DnaDnaAlignFeature::new(-features)
#
my
$dnaf
=
Bio::EnsEMBL::
DnaDnaAlignFeature
->
new
(
-
features
=>
\
@feats
);
$dnaf
->
analysis
(
$f_analysis
);
$ex1
->
add_supporting_features
(
$dnaf
);
$ex2
->
start
(
201372
);
$ex2
->
end
(
201571
);
$ex2
->
phase
(
0
);
$ex2
->
end_phase
(
0
);
$ex2
->
end_phase
(
-
1
);
$ex2
->
contig
(
$slice
);
$ex2
->
strand
(
1
);
$ex2
->
analysis
(
$analysis
);
$ex3
->
start
(
28
);
$ex3
->
end
(
33
);
$ex3
->
phase
(
0
);
$ex3
->
end_phase
(
0
);
@feats
=
();
$fp
=
new
Bio::EnsEMBL::
FeaturePair
;
$fp
->
start
(
201372
);
$fp
->
end
(
201471
);
$fp
->
strand
(
1
);
$fp
->
score
(
10
);
$fp
->
contig
(
$slice
);
$fp
->
hstart
(
100
);
$fp
->
hend
(
199
);
$fp
->
hstrand
(
1
);
$fp
->
hseqname
('
dummy-hid
');
push
(
@feats
,
$fp
);
$fp
=
new
Bio::EnsEMBL::
FeaturePair
;
$fp
->
start
(
201472
);
$fp
->
end
(
201571
);
$fp
->
strand
(
1
);
$fp
->
score
(
10
);
$fp
->
contig
(
$slice
);
$fp
->
seqname
(
1
);
$fp
->
hstart
(
201
);
$fp
->
hend
(
300
);
$fp
->
hstrand
(
1
);
$fp
->
hseqname
('
dummy-hid
');
push
(
@feats
,
$fp
);
#
#
# 2 Test DnaDnaAlignFeature::new(-features)
#
$dnaf
=
Bio::EnsEMBL::
DnaDnaAlignFeature
->
new
(
-
features
=>
\
@feats
);
$dnaf
->
analysis
(
$f_analysis
);
$ex2
->
add_supporting_features
(
$dnaf
);
$ex3
->
start
(
210600
);
$ex3
->
end
(
210800
);
$ex3
->
phase
(
-
1
);
$ex3
->
end_phase
(
-
1
);
$ex3
->
contig
(
$slice
);
$ex3
->
strand
(
1
);
$ex3
->
analysis
(
$analysis
);
...
...
@@ -111,7 +197,7 @@ $transcript1->add_Exon($ex2);
$translation1
->
start_Exon
(
$ex1
);
$translation1
->
end_Exon
(
$ex2
);
$translation1
->
start
(
1
);
$translation1
->
end
(
9
);
$translation1
->
end
(
150
);
$transcript1
->
translation
(
$translation1
);
...
...
@@ -119,9 +205,9 @@ $transcript2->add_Exon($ex1);
$transcript2
->
add_Exon
(
$ex2
);
$transcript2
->
add_Exon
(
$ex3
);
$translation2
->
start_Exon
(
$ex1
);
$translation2
->
end_Exon
(
$ex
3
);
$translation2
->
end_Exon
(
$ex
2
);
$translation2
->
start
(
1
);
$translation2
->
end
(
6
);
$translation2
->
end
(
180
);
$transcript2
->
translation
(
$translation2
);
debug
(
"
Transcripts created
"
);
...
...
@@ -136,8 +222,14 @@ $gene->analysis($analysis);
debug
(
"
Getting all the Transcripts/Exons from new Gene
"
);
my
$count
=
0
;
my
$translates
=
1
;
foreach
my
$tr
(
@
{
$gene
->
get_all_Transcripts
()}
)
{
if
(
$tr
->
translate
()
->
seq
()
=~
/\*./
)
{
$translates
=
0
;
debug
(
"
Translate failed.
"
.
$tr
->
translate
()
->
seq
()
);
}
foreach
my
$exon
(
@
{
$tr
->
get_all_Exons
()}
)
{
debug
(
"
Exon start:
"
.
$exon
->
start
());
debug
(
"
Exon end:
"
.
$exon
->
end
()
);
...
...
@@ -147,11 +239,12 @@ foreach my $tr( @{$gene->get_all_Transcripts()} ) {
}
ok
(
$count
==
5
);
ok
(
$translates
);
ok
(
scalar
(
@
{
$gene
->
get_all_Exons
()}
)
==
3
);
$gene
->
transform
();
$multi
->
hide
(
"
core
",
"
gene
",
"
transcript
",
"
exon
",
"
exon_transcript
",
"
gene_description
",
"
translation
",
"
gene_stable_id
",
"
transcript_stable_id
",
"
exon_stable_id
",
"
translation_stable_id
"
);
$multi
->
hide
(
"
core
",
"
gene
",
"
transcript
",
"
exon
",
"
exon_transcript
",
"
gene_description
",
"
translation
",
"
gene_stable_id
",
"
transcript_stable_id
",
"
exon_stable_id
",
"
translation_stable_id
"
,
"
supporting_feature
",
"
dna_align_feature
"
);
my
$gene_ad
=
$db
->
get_GeneAdaptor
();
debug
(
"
Storing the gene
"
);
...
...
@@ -159,9 +252,7 @@ $gene_ad->store($gene);
ok
(
1
);
my
$new_slice
=
$db
->
get_SliceAdaptor
()
->
fetch_by_chr_start_end
(
"
20
",
30263615
,
30265615
);
my
$genes
=
$new_slice
->
get_all_Genes
();
my
$genes
=
$slice
->
get_all_Genes
();
...
...
@@ -181,9 +272,11 @@ foreach my $tr( @{$gene_out->get_all_Transcripts()} ) {
my
$exons
=
$gene_out
->
get_all_Transcripts
()
->
[
0
]
->
get_all_Exons
();
ok
(
$exons
->
[
0
]
->
start
==
1005
);
ok
(
$exons
->
[
1
]
->
strand
==
1
);
ok
(
$exons
->
[
1
]
->
phase
==
0
);
ok
(
$exons
->
[
0
]
->
start
==
13586
);
ok
(
$exons
->
[
1
]
->
strand
==
1
);
ok
(
$exons
->
[
1
]
->
phase
==
0
);
...
...
@@ -204,7 +297,26 @@ foreach my $trans( @{$gene_out->get_all_Transcripts()} ){
ok
(
$translate
==
1
);
my
$t
=
$gene_out
->
get_all_Transcripts
()
->
[
1
];
my
$e
=
$t
->
get_all_Exons
()
->
[
0
];
my
$se
=
$e
->
get_all_supporting_features
();
debug
(
"
Got
"
.
scalar
(
@$se
)
.
"
supporting features.
"
);
ok
(
scalar
(
@$se
)
==
2
);
my
$se_start
=
$se
->
[
0
]
->
start
()
<
$se
->
[
1
]
->
start
()
?
$se
->
[
0
]
->
start
()
:
$se
->
[
1
]
->
start
();
my
$se_end
=
$se
->
[
0
]
->
end
()
>
$se
->
[
1
]
->
end
()
?
$se
->
[
0
]
->
end
()
:
$se
->
[
1
]
->
end
();
debug
(
"
Supporting start
$se_start
, end
$se_end
"
);
debug
(
"
Exon start
"
.
$e
->
start
()
.
"
end
"
.
$e
->
end
()
);
ok
(
$se_start
==
$e
->
start
()
);
ok
(
$se_end
==
$e
->
end
()
);
my
$pep1
=
$t
->
translate
()
->
seq
();
...
...
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