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ensembl-gh-mirror
ensembl
Commits
d2679435
Commit
d2679435
authored
13 years ago
by
Kieron Taylor
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Minor corrections to pass the time while waiting for the farm to do its thing.
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misc-scripts/xref_mapping/docs/running_the_xref_pipeline.txt
+16
-17
16 additions, 17 deletions
misc-scripts/xref_mapping/docs/running_the_xref_pipeline.txt
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16 additions
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17 deletions
misc-scripts/xref_mapping/docs/running_the_xref_pipeline.txt
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d2679435
...
...
@@ -91,6 +91,9 @@ Good docs can be found at
https://www.ebi.ac.uk/seqdb/confluence/display/ENS/Importing+LRGs+into+Ensembl
which comes down to doing the following :-
Check that the LRG modules are added to perl5lib
so for my instance I set
setenv PERL5LIB ${PERL5LIB}:/nfs/users/nfs_i/ianl/LRG/code/modules
perl scripts/import.lrg.pl -verbose -do_all -host ens-staging -port
3306 -user rw -pass password -core homo_sapiens_core_65_37
...
...
@@ -116,13 +119,10 @@ perl scripts/import.lrg.pl -verbose -do_all -host ens-staging -port
homo_sapiens_cdna_65_37 -vega homo_sapiens_vega_65_37 -rnaseq
homo_sapiens_rnaseq_65_37 -verify >& verify.OUT
need to add modules to perl5lib to know where to find the modules
so for my instance i set
setenv PERL5LIB ${PERL5LIB}:/nfs/users/nfs_i/ianl/LRG/code/modules
If the cdna databses
is
not yet ready then remove the "-cdna
If the cdna datab
a
ses
are
not yet ready then remove the "-cdna
homo_sapiens_cdna_65_37" bit and continue but let who ever is building
this database that you are doing the LRGs so that they get the same
this database
know
that you are doing the LRGs so that they get the same
data.
...
...
@@ -130,8 +130,7 @@ data.
Run the parsing
---------------
More detailed instructions can be found in the FAQ.txt and
More detailed instructions can be found in the FAQ.txt,
but basically you should cd to where you want the files to be downloaded to
and run the following;-
...
...
@@ -163,7 +162,7 @@ Explanation of the output:-
> -dbname ianl_human_xref_65 -species human -stats -
> create -force
Tells us what options were used when the parser script was r
a
n.
Tells us what options were used when the parser script was r
u
n.
> ----{ XXXX }-----------------------------------------------------------------
...
...
@@ -325,7 +324,7 @@ to do next
>No alt_alleles found for this species.
only for human do we i
n
port the alt_alleles
only for human do we i
m
port the alt_alleles
>Dumping xref & Ensembl sequences
...
...
@@ -347,7 +346,7 @@ exist they will not be re dumped.
>already processed = 0, processed = 734, errors = 0, empty = 0
This is information on the mapping of the fasta files using exonerate. Check that
the errors are 0 else one of the mapping went wrong.
the errors are 0 else one of the mapping
s
went wrong.
>Could not find stable id ENSDART00000126968 in table to get the internal id hence
...
...
@@ -367,7 +366,7 @@ this is not a problem.
> ZFIN_ID
Priority xrefs are those xrefs where we get the data from more than one place.
These will have priorit
y
s which tell us which is better so the best ones are
These will have priorit
ie
s which tell us which is better so the best ones are
chosen at this point.
...
...
@@ -403,7 +402,7 @@ highest and Translation the lowest.
>DBASS3 moved to Gene level.
>DBASS5 moved to Gene level.
Some sources are considered to belong to genes but maybe mapped to transcripts or
Some sources are considered to belong to genes but may
be mapped to transcripts or
translations so we move these now to the gene.
...
...
@@ -416,8 +415,8 @@ translations so we move these now to the gene.
> wu:fj89a05 (left as ZFIN_ID reference but not gene symbol)
For some sources (HGNC in human, MGI in mouse and ZFIN_ID in zebrafish) we only
want to have one reference per gene so using things like their priorit
y
s, %id
mapping values etc we try to find the best one and remove the others. If we cannot
want to have one reference per gene so using things like their priorit
ie
s, %id
mapping values etc
.
we try to find the best one and remove the others. If we cannot
find a best one then all are kept.
...
...
@@ -491,7 +490,7 @@ So we report the number and type of xrefs that are loaded.
>Setting Transcript and Gene display_xrefs from xref database into core and
> setting the desc
In the official naming routine which mouse, human and zebrafish run we set
In the official naming routine which mouse, human and zebrafish run
,
we set
the display_xrefs and descriptions.
...
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@@ -513,8 +512,8 @@ Used for checking/debuging mainly.
> RFAM 9
>6437 gene descriptions added
For those that the official naming routine could not set we now add display_xrefs
and decriptions. NOTE: the higher the number the
r
greater the priority for naming.
For those that the official naming routine could not set
,
we now add display_xrefs
and de
s
criptions. NOTE: the higher the number the greater the priority for naming.
>xref_mapper.pl FINISHED NORMALLY
...
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