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Commit e84ebcea authored by Maurice Hendrix's avatar Maurice Hendrix
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slight change to allow automated loutre QC. 

sub check_for_stops can be given a 3rd parameter, an object with the sub _save_log, to save the logs to. This is optional, ommitting it renders the sub functionally equivalent to the previous version
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misc-scripts/alternative_splicing/AltSplicingToolkit/release_notes.txt
+ 113
− 113
View file @ e84ebcea
altSpliceFinder Release Notes - User visible changes.
Release 0.5.2 (03 February 2010)
--------------------------------
* First distribution as part of Ensembl
Release 0.5.1 (15 January 2010)
-------------------------------
* Corrected options to compute statistics (--statistiscs|-s)
Released by Gautier Koscielny, based on stable version 0.5.
Release 0.5 (08 December 2009)
------------------------------
* Major changes in altSpliceFinder 0.5 [stable]
Released by Gautier Koscielny, based on stable version 0.4.
** relax mode: option ``-relax|-r'' to compute cassette exons and mutually
exclusive events with relaxed constraints on flanking exons.
In this mode, flanking exons are required to overlap but the constraints
on the same 3p or 5p end is removed.
** bug on A5SS computation fixed.
** remove spurious STDERR logs on intron retention event
computations.
Release 0.4 (13 May 2009)
-------------------------
* Major changes in altSpliceFinder 0.4 (2009-05-13) [stable]
Released by Gautier Koscielny, based on stable version 0.3.
** GFF output: each feature in featuresA or featuresB is followed by a list
of corresponding transcripts in brackets.
** constitutive mode: option ``-constitutives|-c'' to compute constitutive exons
events only.
** bug on constitutive exon computation fixed.
Release 0.3 (27 January 2009)
-----------------------------
* Major changes in altSpliceFinder 0.3 (2009-01-27) [stable]
Released by Gautier Koscielny, based on stable version 0.2.
** Unique ID for each event based on gene ID and event type:
<gene_id>-<event_type>-number
** Splice sites coordinates added for each feature of each type of events.
** Extension of Intron Retention computations to catch more cases (not only
exact exon 5p/3p but also overlapping cases).
** Log4C++ Verbose mode added.
** GNU Configure program with Log4CPP libraries dependencies.
Release 0.2 (13 January 2009)
-----------------------------
* Major changes in altSpliceFinder 0.2 (2009-01-13) [stable]
Released by Gautier Koscielny, based on stable version 0.1.
** Alternative First Exon (AFE) event computation.
** Alternative Last Exon (ALE) event computation.
** Constitutive Exon (CNE) event computation module added.
** Source code directory structure reflects C++ namespace structure.
** Replace the traditional Makefile with a GNU Configure program
including Boost libraries dependencies.
** GPL license added.
release 0.1 (December 2008)
---------------------------
* First release by Gautier Koscielny
** Cassette Exon (CE) event computation.
** Intron Isoform (II) event computation.
** Exon Isoform (EI) event computation.
** Intron Retention (IR) event computation.
** Mutually Exclusive (MXE) event computation.
** Alternative Initiation (AI) event computation.
** Alternative Termination (AT) event computation.
** Simple makefile.
-----
Copyright (c) 1999-2010 The European Bioinformatics Institute and
Genome Research Limited, and others. All rights reserved.
This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
Ensembl License for more details.
You should have received a copy of the Ensembl License
along with this program. If not, see <http://www.ensembl.org/info/about/code_licence.html>.
altSpliceFinder Release Notes - User visible changes.
Release 0.5.2 (03 February 2010)
--------------------------------
* First distribution as part of Ensembl
Release 0.5.1 (15 January 2010)
-------------------------------
* Corrected options to compute statistics (--statistiscs|-s)
Released by Gautier Koscielny, based on stable version 0.5.
Release 0.5 (08 December 2009)
------------------------------
* Major changes in altSpliceFinder 0.5 [stable]
Released by Gautier Koscielny, based on stable version 0.4.
** relax mode: option ``-relax|-r'' to compute cassette exons and mutually
exclusive events with relaxed constraints on flanking exons.
In this mode, flanking exons are required to overlap but the constraints
on the same 3p or 5p end is removed.
** bug on A5SS computation fixed.
** remove spurious STDERR logs on intron retention event
computations.
Release 0.4 (13 May 2009)
-------------------------
* Major changes in altSpliceFinder 0.4 (2009-05-13) [stable]
Released by Gautier Koscielny, based on stable version 0.3.
** GFF output: each feature in featuresA or featuresB is followed by a list
of corresponding transcripts in brackets.
** constitutive mode: option ``-constitutives|-c'' to compute constitutive exons
events only.
** bug on constitutive exon computation fixed.
Release 0.3 (27 January 2009)
-----------------------------
* Major changes in altSpliceFinder 0.3 (2009-01-27) [stable]
Released by Gautier Koscielny, based on stable version 0.2.
** Unique ID for each event based on gene ID and event type:
<gene_id>-<event_type>-number
** Splice sites coordinates added for each feature of each type of events.
** Extension of Intron Retention computations to catch more cases (not only
exact exon 5p/3p but also overlapping cases).
** Log4C++ Verbose mode added.
** GNU Configure program with Log4CPP libraries dependencies.
Release 0.2 (13 January 2009)
-----------------------------
* Major changes in altSpliceFinder 0.2 (2009-01-13) [stable]
Released by Gautier Koscielny, based on stable version 0.1.
** Alternative First Exon (AFE) event computation.
** Alternative Last Exon (ALE) event computation.
** Constitutive Exon (CNE) event computation module added.
** Source code directory structure reflects C++ namespace structure.
** Replace the traditional Makefile with a GNU Configure program
including Boost libraries dependencies.
** GPL license added.
release 0.1 (December 2008)
---------------------------
* First release by Gautier Koscielny
** Cassette Exon (CE) event computation.
** Intron Isoform (II) event computation.
** Exon Isoform (EI) event computation.
** Intron Retention (IR) event computation.
** Mutually Exclusive (MXE) event computation.
** Alternative Initiation (AI) event computation.
** Alternative Termination (AT) event computation.
** Simple makefile.
-----
Copyright (c) 1999-2010 The European Bioinformatics Institute and
Genome Research Limited, and others. All rights reserved.
This program is distributed in the hope that it will be useful,
but WITHOUT ANY WARRANTY; without even the implied warranty of
MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. See the
Ensembl License for more details.
You should have received a copy of the Ensembl License
along with this program. If not, see <http://www.ensembl.org/info/about/code_licence.html>.
misc-scripts/alternative_splicing/AltSplicingToolkit/src/Main.cpp
+ 212
− 212
View file @ e84ebcea
......@@ -43,216 +43,216 @@
* (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE
* OF THIS SOFTWARE, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.
*/
#include <string>
#include <fstream>
#include <iostream>
#include <sstream>
#include <iterator>
#include <vector>
#include <map>
#include <utility> // useful for std::pair
#include <algorithm>
#include <boost/regex.hpp>
#include <boost/algorithm/string/regex.hpp>
#include <boost/program_options.hpp>
#include "log4cpp/Appender.hh"
#include "log4cpp/OstreamAppender.hh"
#include "log4cpp/Layout.hh"
#include "log4cpp/BasicLayout.hh"
#include <log4cpp/PatternLayout.hh>
#include "log4cpp/Priority.hh"
#include "log4cpp/Category.hh"
//#include <boost/filesystem/path.hpp>
//#include <boost/filesystem/fstream.hpp>
//#include <boost/tokenizer.hpp>
// for std::for_each
#include "Constants.h"
#include "gff/GffParser.h"
#include "gff/GffSimpleHandler.h"
#include "gff/BioMartGffHandler.h"
#include "gff/SplicingEventGffGenerator.h"
using namespace std;
using namespace boost;
using namespace as;
using namespace gff;
namespace po = boost::program_options;
void outputUsage(const po::options_description& desc)
{
std::cout << "altSpliceFinder 0.5.2\n" << endl
//<< as::version::Version::getApplicationVersion()
<< "Copyright (c) 2008, 2009, 2010 Gautier Koscielny <koscieln@ebi.ac.uk>" << endl
<< "The European Bioinformatics Institute and" << endl
#include <string>
#include <fstream>
#include <iostream>
#include <sstream>
#include <iterator>
#include <vector>
#include <map>
#include <utility> // useful for std::pair
#include <algorithm>
#include <boost/regex.hpp>
#include <boost/algorithm/string/regex.hpp>
#include <boost/program_options.hpp>
#include "log4cpp/Appender.hh"
#include "log4cpp/OstreamAppender.hh"
#include "log4cpp/Layout.hh"
#include "log4cpp/BasicLayout.hh"
#include <log4cpp/PatternLayout.hh>
#include "log4cpp/Priority.hh"
#include "log4cpp/Category.hh"
//#include <boost/filesystem/path.hpp>
//#include <boost/filesystem/fstream.hpp>
//#include <boost/tokenizer.hpp>
// for std::for_each
#include "Constants.h"
#include "gff/GffParser.h"
#include "gff/GffSimpleHandler.h"
#include "gff/BioMartGffHandler.h"
#include "gff/SplicingEventGffGenerator.h"
using namespace std;
using namespace boost;
using namespace as;
using namespace gff;
namespace po = boost::program_options;
void outputUsage(const po::options_description& desc)
{
std::cout << "altSpliceFinder 0.5.2\n" << endl
//<< as::version::Version::getApplicationVersion()
<< "Copyright (c) 2008, 2009, 2010 Gautier Koscielny <koscieln@ebi.ac.uk>" << endl
<< "The European Bioinformatics Institute and" << endl
<< "Genome Research Limited, and others." << endl
<< "All rights reserved.\n\n";
// notice
// usage
std::cout << "Usage:" << endl << endl;
std::cout << " altSpliceFinder [-i gfffile] [-o resultfile]" << endl << endl;
std::cout << desc << endl;
std::cout << "Notes:"<< endl << " Reads from the standard input stream if no input file is given." << endl
<< " Writes to the standard output stream if no output file is given." << endl;
}
int main(int argc, char **argv, char **ppenv)
{
/**
* Read program options
*/
string inputFile;
string outputFile;
po::options_description desc("Allowed options");
desc.add_options()
("version,V", "print version string")
("help,h", "Produce help message")
("verbose,v", "Log computational details on stderr")
("inputFile,i", po::value<std::string>(&inputFile), "Provide a path to a Ensembl BioMart gff file that contains transcript structure")
("outputFile,o", po::value<std::string>(&outputFile), "Provide a path to a gff file that will contains the splicing events")
("limit,l", po::value<int>(), "set a limit on the number of genes (with splicing events) to parse")
("constitutives,c", "Compute constitutive exon events only")
("relax,r", "Compute splicing events with relaxed constraints on flanking features")
("statistics,s", "Compute and show statistics on splicing events");
//("verbose", value<string>()->zero_tokens(), "verbosity level");
//("listRejectedGenes,lrg", value<string>()->zero_tokens(), "store genes with variants but no events in a file");
po::variables_map vm;
po::store(po::parse_command_line(argc, argv, desc), vm);
po::notify(vm);
// argc < 2
if (vm.count("help") || vm.count("version") || (argc > 1 && (!strcmp(argv[1], "-?") || !strcmp(argv[1], "--?") || !strcmp(argv[1], "/?") || !strcmp(argv[1], "/h") || !strcmp(argv[1], "-h") || !strcmp(argv[1], "--h") || !strcmp(argv[1], "--help") || !strcmp(argv[1], "/help") || !strcmp(argv[1], "-help") || !strcmp(argv[1], "help")) ))
{
outputUsage(desc);
return 1;
}
/*
* Log for C++ Appender declaration
*/
log4cpp::Appender* appender; // = Logger.getAppender();
appender = new log4cpp::OstreamAppender("default", &std::cerr);
log4cpp::PatternLayout* patternLayout = new log4cpp::PatternLayout();
patternLayout->setConversionPattern("%d{%H:%M:%S:%l} %c: %m\n"); //"%R %p %c %x: %m\n");
appender->setLayout(patternLayout);
//appender->setLayout(new log4cpp::BasicLayout());
log4cpp::Category& root = log4cpp::Category::getRoot();
root.addAppender(appender);
if (vm.count("verbose")) {
root.setPriority(log4cpp::Priority::INFO);
root.info("Switch to verbose mode.");
} else {
root.setPriority(log4cpp::Priority::ERROR);
}
ifstream inStream;
ofstream outStream;
bool inFile = false;
bool outFile = false;
int limit = (vm.count("limit")) ? vm["limit"].as<int>() : 0;
if (vm.count("inputFile"))
{
inFile = true;
root.infoStream() << "reading filename " << inputFile << log4cpp::eol;
// ok, just try to open it and then close it
inStream.open(inputFile.c_str(), ifstream::in);
if(inStream.fail())
{
inStream.clear(ios::failbit);
inStream.close();
root.errorStream() << "Error:\tinput file """ << inputFile.c_str() << """ does not exists. Exiting program." << log4cpp::eol;
exit(1);
}
} else {
// check cin
if(std::cin.fail()) {
root.errorStream() << "Error:\tno data on input stream. Exiting program." << log4cpp::eol;
exit(1);
}
}
if (vm.count("outputFile"))
{
outFile = true;
outStream.open(outputFile.c_str(), ios::out); //, ios_base::out );
if(outStream.fail()) {
outStream.clear(ios::failbit);
outStream.close();
cerr << "Error:\t enable to create output file """ << outputFile.c_str() << """." << endl;
exit(1);
}
}
SplicingEventGffGenerator gffGenerator((outFile) ? outStream : std::cout, "Ensembl", vm.count("constitutives"), vm.count("relax"));
BioMartGffHandler martHandler(limit);
martHandler.registerNewGeneEventListener(&gffGenerator);
GffParser parser((inFile) ? inStream : std::cin, &martHandler);
parser.parse();
if (inFile)
inStream.close();
if (outFile)
outStream.close();
root.info("Parsing done.");
if (vm.count("statistics")) {
cerr << "Genes parsed:\t\t\t" << gffGenerator.getGeneCount() << endl;
cerr << "Genes with multiple transcripts:\t" << gffGenerator.getGenesWithSeveralTranscriptsCount() << endl;
cerr << "Genes with events:\t\t\t" << gffGenerator.getGenesWithEventsCount() << endl;
cerr << "Splicing Events:\t\t\t" << gffGenerator.getEventCount() << endl;
if (gffGenerator.getEventCount() > 0) {
cerr << "Alternative Initiation Events:\t" << gffGenerator.getCountAI() << " (" << ((int) ((100*gffGenerator.getCountAI())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Alternative Termination Events:\t" << gffGenerator.getCountAT() << " (" << ((int) ((100*gffGenerator.getCountAT())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Alternative First Exon Events:\t" << gffGenerator.getCountAFE() << " (" << ((int) ((100*gffGenerator.getCountAFE())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Alternative Last Exon Events:\t" << gffGenerator.getCountALE() << " (" << ((int) ((100*gffGenerator.getCountALE())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Exon Isoform Events:\t" << gffGenerator.getCountEI() << " (" << ((int) ((100*gffGenerator.getCountEI())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Intron Isoform Events:\t" << gffGenerator.getCountII() << " (" << ((int) ((100*gffGenerator.getCountII())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Intron Retention Events:\t" << gffGenerator.getCountIR() << " (" << ((int) ((100*gffGenerator.getCountIR())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Cassette Exon Events:\t" << gffGenerator.getCountCE() << " (" << ((int) ((100*gffGenerator.getCountCE())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Mutually Exclusive Events:\t" << gffGenerator.getCountMXE() << " (" << ((int) ((100*gffGenerator.getCountMXE())/gffGenerator.getEventCount())) << "%)" << endl ;
}
}
log4cpp::Category::shutdown();
return (EXIT_SUCCESS);
}
<< "All rights reserved.\n\n";
// notice
// usage
std::cout << "Usage:" << endl << endl;
std::cout << " altSpliceFinder [-i gfffile] [-o resultfile]" << endl << endl;
std::cout << desc << endl;
std::cout << "Notes:"<< endl << " Reads from the standard input stream if no input file is given." << endl
<< " Writes to the standard output stream if no output file is given." << endl;
}
int main(int argc, char **argv, char **ppenv)
{
/**
* Read program options
*/
string inputFile;
string outputFile;
po::options_description desc("Allowed options");
desc.add_options()
("version,V", "print version string")
("help,h", "Produce help message")
("verbose,v", "Log computational details on stderr")
("inputFile,i", po::value<std::string>(&inputFile), "Provide a path to a Ensembl BioMart gff file that contains transcript structure")
("outputFile,o", po::value<std::string>(&outputFile), "Provide a path to a gff file that will contains the splicing events")
("limit,l", po::value<int>(), "set a limit on the number of genes (with splicing events) to parse")
("constitutives,c", "Compute constitutive exon events only")
("relax,r", "Compute splicing events with relaxed constraints on flanking features")
("statistics,s", "Compute and show statistics on splicing events");
//("verbose", value<string>()->zero_tokens(), "verbosity level");
//("listRejectedGenes,lrg", value<string>()->zero_tokens(), "store genes with variants but no events in a file");
po::variables_map vm;
po::store(po::parse_command_line(argc, argv, desc), vm);
po::notify(vm);
// argc < 2
if (vm.count("help") || vm.count("version") || (argc > 1 && (!strcmp(argv[1], "-?") || !strcmp(argv[1], "--?") || !strcmp(argv[1], "/?") || !strcmp(argv[1], "/h") || !strcmp(argv[1], "-h") || !strcmp(argv[1], "--h") || !strcmp(argv[1], "--help") || !strcmp(argv[1], "/help") || !strcmp(argv[1], "-help") || !strcmp(argv[1], "help")) ))
{
outputUsage(desc);
return 1;
}
/*
* Log for C++ Appender declaration
*/
log4cpp::Appender* appender; // = Logger.getAppender();
appender = new log4cpp::OstreamAppender("default", &std::cerr);
log4cpp::PatternLayout* patternLayout = new log4cpp::PatternLayout();
patternLayout->setConversionPattern("%d{%H:%M:%S:%l} %c: %m\n"); //"%R %p %c %x: %m\n");
appender->setLayout(patternLayout);
//appender->setLayout(new log4cpp::BasicLayout());
log4cpp::Category& root = log4cpp::Category::getRoot();
root.addAppender(appender);
if (vm.count("verbose")) {
root.setPriority(log4cpp::Priority::INFO);
root.info("Switch to verbose mode.");
} else {
root.setPriority(log4cpp::Priority::ERROR);
}
ifstream inStream;
ofstream outStream;
bool inFile = false;
bool outFile = false;
int limit = (vm.count("limit")) ? vm["limit"].as<int>() : 0;
if (vm.count("inputFile"))
{
inFile = true;
root.infoStream() << "reading filename " << inputFile << log4cpp::eol;
// ok, just try to open it and then close it
inStream.open(inputFile.c_str(), ifstream::in);
if(inStream.fail())
{
inStream.clear(ios::failbit);
inStream.close();
root.errorStream() << "Error:\tinput file """ << inputFile.c_str() << """ does not exists. Exiting program." << log4cpp::eol;
exit(1);
}
} else {
// check cin
if(std::cin.fail()) {
root.errorStream() << "Error:\tno data on input stream. Exiting program." << log4cpp::eol;
exit(1);
}
}
if (vm.count("outputFile"))
{
outFile = true;
outStream.open(outputFile.c_str(), ios::out); //, ios_base::out );
if(outStream.fail()) {
outStream.clear(ios::failbit);
outStream.close();
cerr << "Error:\t enable to create output file """ << outputFile.c_str() << """." << endl;
exit(1);
}
}
SplicingEventGffGenerator gffGenerator((outFile) ? outStream : std::cout, "Ensembl", vm.count("constitutives"), vm.count("relax"));
BioMartGffHandler martHandler(limit);
martHandler.registerNewGeneEventListener(&gffGenerator);
GffParser parser((inFile) ? inStream : std::cin, &martHandler);
parser.parse();
if (inFile)
inStream.close();
if (outFile)
outStream.close();
root.info("Parsing done.");
if (vm.count("statistics")) {
cerr << "Genes parsed:\t\t\t" << gffGenerator.getGeneCount() << endl;
cerr << "Genes with multiple transcripts:\t" << gffGenerator.getGenesWithSeveralTranscriptsCount() << endl;
cerr << "Genes with events:\t\t\t" << gffGenerator.getGenesWithEventsCount() << endl;
cerr << "Splicing Events:\t\t\t" << gffGenerator.getEventCount() << endl;
if (gffGenerator.getEventCount() > 0) {
cerr << "Alternative Initiation Events:\t" << gffGenerator.getCountAI() << " (" << ((int) ((100*gffGenerator.getCountAI())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Alternative Termination Events:\t" << gffGenerator.getCountAT() << " (" << ((int) ((100*gffGenerator.getCountAT())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Alternative First Exon Events:\t" << gffGenerator.getCountAFE() << " (" << ((int) ((100*gffGenerator.getCountAFE())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Alternative Last Exon Events:\t" << gffGenerator.getCountALE() << " (" << ((int) ((100*gffGenerator.getCountALE())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Exon Isoform Events:\t" << gffGenerator.getCountEI() << " (" << ((int) ((100*gffGenerator.getCountEI())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Intron Isoform Events:\t" << gffGenerator.getCountII() << " (" << ((int) ((100*gffGenerator.getCountII())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Intron Retention Events:\t" << gffGenerator.getCountIR() << " (" << ((int) ((100*gffGenerator.getCountIR())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Cassette Exon Events:\t" << gffGenerator.getCountCE() << " (" << ((int) ((100*gffGenerator.getCountCE())/gffGenerator.getEventCount())) << "%)" << endl ;
cerr << "Mutually Exclusive Events:\t" << gffGenerator.getCountMXE() << " (" << ((int) ((100*gffGenerator.getCountMXE())/gffGenerator.getEventCount())) << "%)" << endl ;
}
}
log4cpp::Category::shutdown();
return (EXIT_SUCCESS);
}
modules/Bio/EnsEMBL/Utils/VegaCuration/Translation.pm
+ 9
− 9
View file @ e84ebcea
......@@ -284,11 +284,11 @@ sub check_for_stops {
if ($num_tga < $num_stops) {
if ($source eq 'havana') {
#$support->log_warning("INTERNAL STOPS HAVANA: Transcript $tsi ($tname) from gene $gname has non \'$scodon\' stop codons [$mod_date]:\nSequence = $orig_seq\nStops at $positions)\n\n");
$log_object->_save_log('log_warning', '', $gsi, '', $tsi, 'VQCT_internal_stop', "INTERNAL STOPS HAVANA: Transcript $tsi ($tname) from gene $gname has non \'$scodon\' stop codons [$mod_date]:\nSequence = $orig_seq\nStops at $positions)\n\n");
$log_object->_save_log('log_warning', '', $gsi, 'TRANSCRIPT', $tsi, 'VQCT_internal_stop', "INTERNAL STOPS HAVANA: Transcript $tsi ($tname) from gene $gname has non \'$scodon\' stop codons [$mod_date]: Sequence = $orig_seq Stops at $positions)");
}
else {
#$support->log_warning("INTERNAL STOPS EXTERNAL: Transcript $tsi ($tname) from gene $gname has non \'$scodon\' stop codons[$mod_date]:\nSequence = $orig_seq\nStops at $positions)\n\n");
$log_object->_save_log('log_warning', '', $gsi, '', $tsi, 'VQCT_internal_stop', "INTERNAL STOPS EXTERNAL: Transcript $tsi ($tname) from gene $gname has non \'$scodon\' stop codons[$mod_date]:\nSequence = $orig_seq\nStops at $positions)\n\n");
$log_object->_save_log('log_warning', '', $gsi, 'TRANSCRIPT', $tsi, 'VQCT_internal_stop', "INTERNAL STOPS EXTERNAL: Transcript $tsi ($tname) from gene $gname has non \'$scodon\' stop codons[$mod_date]: Sequence = $orig_seq Stops at $positions)");
}
}
......@@ -313,7 +313,7 @@ sub check_for_stops {
foreach my $text (@{$remarks}) {
if ( ($attrib eq 'remark') && ($text=~/^$alabel(.*)/) ){
#$support->log_warning("seleno remark for $tsi stored as Annotation_remark not hidden remark) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, '', $tsi, 'VQCT_wrong_selC_coord', "seleno remark for $tsi stored as Annotation_remark not hidden remark) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, '', $tsi, 'VQCT_wrong_selC_coord', "seleno remark for $tsi stored as Annotation_remark not hidden remark) [$mod_date]");
$annot_stops=$1;
}
elsif ($text =~ /^$alabel2(.*)/) {
......@@ -337,16 +337,16 @@ sub check_for_stops {
# catch old annotations where number was in DNA not peptide coordinates
elsif (($found_stops[$i]->[1] * 3) == $offset) {
#$support->log_warning("DNA: Annotated stop for transcript tsi ($tname) is in DNA not peptide coordinates) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, '', $tsi, '', "DNA: Annotated stop for transcript tsi ($tname) is in DNA not peptide coordinates) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, 'DNA', $tsi, 'VQCT_wrong_selC_coord', "DNA: Annotated stop for transcript tsi ($tname) is in DNA not peptide coordinates) [$mod_date]");
}
# catch old annotations where number off by one
elsif (($found_stops[$i]->[1]) == $offset+1) {
#$support->log_warning("PEPTIDE: Annotated stop for transcript $tsi ($tname) is out by one) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, '', $tsi, '', "PEPTIDE: Annotated stop for transcript $tsi ($tname) is out by one) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, 'PEPTIDE', $tsi, 'VQCT_wrong_selC_coord', "PEPTIDE: Annotated stop for transcript $tsi ($tname) is out by one) [$mod_date]");
}
else {
#$support->log_warning("Annotated stop for transcript $tsi ($tname) does not match a TGA codon) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, '', $tsi, '', "Annotated stop for transcript $tsi ($tname) does not match a TGA codon) [$mod_date]\n");
$log_object->_save_log('log_warning', '', $gsi, 'TRANSCRIPT', $tsi, 'VQCT_wrong_selC_coord', "Annotated stop for transcript $tsi ($tname) does not match a TGA codon) [$mod_date]");
push @annotated_stops, $offset;
}
$i++;
......@@ -359,12 +359,12 @@ sub check_for_stops {
my $seq = $stop->[0];
unless ( grep { $pos == $_} @annotated_stops) {
if ($seen_transcripts->{$tsi}) {
#$support->log_verbose("Transcript $tsi ($tname) has potential selenocysteines but has been discounted by annotators:\n\t".$seen_transcripts->{$tsi}.") [$mod_date]\n");
$log_object->_save_log('log_verbose', '', $gsi, '', $tsi, 'VQCT_pot_selC', "Transcript $tsi ($tname) has potential selenocysteines but has been discounted by annotators:\n\t".$seen_transcripts->{$tsi}.") [$mod_date]\n");
#$support->log_verbose("Transcript $tsi ($tname) has potential selenocysteines but has been discounted by annotators:\n".$seen_transcripts->{$tsi}.") [$mod_date]\n");
$log_object->_save_log('log_verbose', '', $gsi, '', $tsi, 'VQCT_pot_selC', "Transcript $tsi ($tname) has potential selenocysteines but has been discounted by annotators:\n".$seen_transcripts->{$tsi}.") [$mod_date]");
}
else {
#$support->log("POTENTIAL SELENO ($seq) in $tsi ($tname, gene $gname) found at $pos [$mod_date]\n");
$log_object->_save_log('log', '', $gsi, '', $tsi, 'VQCT_pot_selC', "POTENTIAL SELENO ($seq) in $tsi ($tname, gene $gname) found at $pos [$mod_date]\n");
$log_object->_save_log('log', '', $gsi, '', $tsi, 'VQCT_pot_selC', "POTENTIAL SELENO ($seq) in $tsi ($tname, gene $gname) found at $pos [$mod_date]");
}
}
}
......
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